AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0232690

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0232690 MtrunA17_Chr2g0280141 89.359 827 55 4 1 803 1 818 0.0 1400
Msa0232690 MtrunA17_Chr2g0325811 45.204 834 363 16 19 801 40 830 1.32e-180 539
Msa0232690 MtrunA17_Chr2g0325711 42.962 817 393 15 10 783 31 817 2.40e-178 533
Msa0232690 MtrunA17_Chr2g0325661 42.938 885 354 19 16 802 45 876 2.67e-165 501
Msa0232690 MtrunA17_Chr2g0325861 42.131 826 324 14 6 727 71 846 2.78e-161 490
Msa0232690 MtrunA17_Chr2g0325361 48.505 602 262 7 248 802 477 1077 7.90e-137 432
Msa0232690 MtrunA17_Chr2g0325361 35.636 550 295 8 25 529 13 548 4.82e-59 218
Msa0232690 MtrunA17_Chr6g0471251 39.083 829 348 19 19 802 51 767 2.69e-134 417
Msa0232690 MtrunA17_Chr2g0325321 41.365 747 351 12 42 704 18 761 1.02e-126 397
Msa0232690 MtrunA17_Chr2g0325611 51.599 469 187 9 359 802 137 590 1.66e-124 386
Msa0232690 MtrunA17_Chr2g0325611 38.023 263 106 7 188 427 93 321 1.56e-26 115
Msa0232690 MtrunA17_Chr2g0325231 36.637 898 357 22 19 721 16 896 2.49e-124 395
Msa0232690 MtrunA17_Chr2g0325351 45.804 572 267 11 247 802 144 688 2.57e-116 367
Msa0232690 MtrunA17_Chr2g0325351 36.937 333 140 6 93 414 142 415 9.18e-33 135
Msa0232690 MtrunA17_Chr2g0325351 34.899 298 156 7 179 456 136 415 1.14e-23 107
Msa0232690 MtrunA17_Chr2g0325621 42.652 558 275 8 272 802 121 660 5.56e-109 347
Msa0232690 MtrunA17_Chr2g0325621 34.247 438 201 8 12 415 3 387 1.05e-37 150
Msa0232690 MtrunA17_Chr5g0434761 61.132 265 99 4 541 802 2 265 5.72e-92 289
Msa0232690 MtrunA17_Chr2g0325701 38.403 526 237 11 183 701 144 589 4.06e-78 263
Msa0232690 MtrunA17_Chr2g0325631 59.245 265 104 4 541 802 2 265 1.08e-76 249
Msa0232690 MtrunA17_Chr3g0140591 74.405 168 42 1 537 704 6 172 2.13e-75 242
Msa0232690 MtrunA17_Chr2g0325281 36.393 610 291 11 15 539 3 600 1.41e-73 251
Msa0232690 MtrunA17_Chr2g0325221 35.852 569 292 9 19 530 45 597 5.73e-72 249
Msa0232690 MtrunA17_Chr2g0325221 61.386 101 38 1 702 801 599 699 3.71e-28 121
Msa0232690 MtrunA17_Chr5g0416271 38.446 489 245 10 216 701 47 482 6.53e-71 241
Msa0232690 MtrunA17_Chr5g0416271 33.832 334 178 8 43 364 14 316 2.45e-26 113
Msa0232690 MtrunA17_Chr2g0325291 64.878 205 69 3 556 758 3 206 4.51e-70 230
Msa0232690 MtrunA17_Chr2g0333341 45.808 334 136 7 389 721 332 621 2.50e-64 227
Msa0232690 MtrunA17_Chr2g0333341 35.096 416 189 12 25 396 72 450 1.14e-37 150
Msa0232690 MtrunA17_Chr4g0044221 57.672 189 79 1 82 269 1 189 2.87e-55 193
Msa0232690 MtrunA17_Chr4g0044221 63.226 155 28 2 486 639 168 294 2.16e-54 191
Msa0232690 MtrunA17_Chr2g0325241 41.846 325 161 6 312 627 120 425 3.64e-52 187
Msa0232690 MtrunA17_Chr2g0325241 37.073 205 124 2 248 449 126 328 2.76e-20 94.4
Msa0232690 MtrunA17_Chr2g0332961 42.857 322 136 11 368 674 65 353 5.88e-48 174
Msa0232690 MtrunA17_Chr2g0325721 35.094 530 241 12 22 505 29 501 4.94e-47 175
Msa0232690 MtrunA17_Chr2g0325721 31.635 373 208 9 173 512 108 466 6.82e-19 90.9
Msa0232690 MtrunA17_Chr2g0332951 47.807 228 94 8 483 704 2 210 2.47e-44 159
Msa0232690 MtrunA17_Chr2g0325761 31.029 651 329 13 21 606 53 648 3.63e-44 170
Msa0232690 MtrunA17_Chr2g0325761 62.245 98 36 1 701 797 591 688 2.58e-28 121
Msa0232690 MtrunA17_Chr2g0325771 58.046 174 61 3 534 704 11 175 6.89e-42 150
Msa0232690 MtrunA17_Chr8g0361651 34.673 398 231 11 417 800 23 405 9.95e-41 156
Msa0232690 MtrunA17_Chr2g0325331 58.170 153 62 2 542 693 3 154 1.83e-38 140
Msa0232690 MtrunA17_Chr4g0024061 30.395 658 345 26 191 800 59 651 2.04e-38 153
Msa0232690 MtrunA17_Chr2g0325691 34.120 466 218 13 122 527 283 719 8.98e-38 151
Msa0232690 MtrunA17_Chr2g0325691 37.048 332 192 4 185 512 166 484 1.55e-31 132
Msa0232690 MtrunA17_Chr2g0325691 37.500 272 148 4 296 552 223 487 1.45e-25 113
Msa0232690 MtrunA17_Chr2g0325651 34.359 390 158 8 56 409 59 386 1.23e-37 145
Msa0232690 MtrunA17_Chr2g0325651 45.517 145 69 2 389 526 236 377 2.96e-22 99.8
Msa0232690 MtrunA17_Chr2g0325651 45.033 151 72 2 375 514 236 386 1.56e-21 97.8
Msa0232690 MtrunA17_Chr7g0235901 36.034 358 170 5 61 415 10 311 7.72e-36 138
Msa0232690 MtrunA17_Chr7g0235901 36.406 217 100 2 296 512 132 310 1.67e-20 93.2
Msa0232690 MtrunA17_Chr7g0235901 33.673 196 125 2 339 530 92 286 1.05e-12 70.1
Msa0232690 MtrunA17_Chr2g0325311 63.107 103 36 2 702 802 17 119 1.47e-31 119
Msa0232690 MtrunA17_Chr2g0325641 37.091 275 166 1 249 523 1 268 3.62e-30 122
Msa0232690 MtrunA17_Chr2g0325641 36.336 333 189 5 212 538 3 318 1.12e-29 120
Msa0232690 MtrunA17_Chr2g0325641 37.795 254 133 5 296 542 6 241 5.32e-23 100
Msa0232690 MtrunA17_Chr2g0325641 35.385 260 130 5 190 418 58 310 1.34e-22 99.8
Msa0232690 MtrunA17_Chr3g0140581 55.147 136 59 1 92 227 21 154 1.48e-29 115
Msa0232690 MtrunA17_Chr2g0325271 54.808 104 45 2 701 802 40 143 5.25e-27 107
Msa0232690 MtrunA17_Chr2g0325201 35.865 237 137 2 118 354 2 223 3.57e-26 107
Msa0232690 MtrunA17_Chr2g0325261 32.588 313 125 6 56 333 45 306 1.38e-23 102
Msa0232690 MtrunA17_Chr2g0325261 51.220 82 40 0 375 456 222 303 2.28e-12 68.9
Msa0232690 MtrunA17_Chr2g0325261 31.556 225 142 4 292 512 87 303 6.60e-11 64.3
Msa0232690 MtrunA17_Chr2g0325341 32.588 313 125 6 56 333 45 306 1.38e-23 102
Msa0232690 MtrunA17_Chr2g0325341 51.220 82 40 0 375 456 222 303 2.28e-12 68.9
Msa0232690 MtrunA17_Chr2g0325341 31.556 225 142 4 292 512 87 303 6.60e-11 64.3
Msa0232690 MtrunA17_Chr5g0434751 37.500 280 147 2 249 514 1 266 1.07e-22 99.4
Msa0232690 MtrunA17_Chr5g0434751 35.565 239 136 3 188 419 35 262 7.00e-16 79.3
Msa0232690 MtrunA17_Chr5g0434751 38.073 218 121 2 248 458 56 266 2.38e-15 77.8
Msa0232690 MtrunA17_Chr2g0325781 31.921 354 125 9 16 341 40 305 2.80e-22 98.6
Msa0232690 MtrunA17_Chr2g0325781 42.241 116 43 1 296 411 214 305 4.17e-12 68.2
Msa0232690 MtrunA17_Chr5g0434741 31.373 306 123 7 56 326 46 299 3.06e-22 98.2
Msa0232690 MtrunA17_Chr7g0235921 59.794 97 38 1 618 714 1 96 2.10e-20 87.0
Msa0232690 MtrunA17_Chr4g0053711 32.143 252 145 8 576 803 194 443 4.43e-20 94.7
Msa0232690 MtrunA17_Chr2g0325751 42.038 157 42 3 249 405 7 114 7.51e-17 78.6
Msa0232690 MtrunA17_Chr2g0325751 36.242 149 94 1 403 550 7 155 5.64e-16 76.3
Msa0232690 MtrunA17_Chr2g0325751 46.087 115 55 2 305 419 7 114 3.67e-14 70.9
Msa0232690 MtrunA17_Chr2g0325791 46.825 126 57 1 310 435 41 156 1.75e-16 81.6
Msa0232690 MtrunA17_Chr2g0325791 39.683 126 66 1 352 477 41 156 3.05e-13 71.6
Msa0232690 MtrunA17_Chr2g0325741 29.395 347 117 12 16 318 42 304 4.03e-16 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0232690 AT5G27220.1 28.079 203 134 7 581 778 872 1067 4.13e-15 80.5
Msa0232690 AT5G27220.1 25.542 646 406 20 76 697 208 802 9.87e-14 75.9
Msa0232690 AT5G27220.3 28.079 203 134 7 581 778 872 1067 4.13e-15 80.5
Msa0232690 AT5G27220.3 25.542 646 406 20 76 697 208 802 9.87e-14 75.9
Msa0232690 AT5G27220.4 25.542 646 406 20 76 697 208 802 6.22e-14 76.6
Msa0232690 AT5G27220.4 27.815 151 101 4 630 778 868 1012 5.77e-12 70.1
Msa0232690 AT5G27220.2 25.542 646 406 20 76 697 208 802 6.22e-14 76.6
Msa0232690 AT5G27220.2 27.815 151 101 4 630 778 868 1012 5.77e-12 70.1
Msa0232690 AT1G31814.1 26.786 224 150 6 569 783 117 335 1.85e-13 73.9
Msa0232690 AT5G48385.1 27.333 150 108 1 655 803 308 457 2.58e-13 73.9
Msa0232690 AT5G27230.1 27.056 377 212 16 423 755 38 395 3.92e-13 73.9

Find 162 sgRNAs with CRISPR-Local

Find 249 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCATTGTCAACTTTGAATT+TGG 0.089700 2_2:+4953236 Msa0232690:CDS
TTGCTCCTTCTGTCAATTTA+TGG 0.102499 2_2:+4955200 Msa0232690:CDS
GATAGATAGTATTATAATTT+TGG 0.129006 2_2:+4956448 Msa0232690:three_prime_UTR
CCAAAGTGAAACACTGTATT+TGG 0.182293 2_2:-4952505 None:intergenic
GGTTTGGCACATAAAGTTTC+TGG 0.195386 2_2:+4955314 Msa0232690:CDS
GCTCTTCTTCATGAGCTTAA+TGG 0.209076 2_2:-4953388 None:intergenic
CAGGATTCTGCGTTAAGATT+TGG 0.231497 2_2:+4956402 Msa0232690:three_prime_UTR
TGTAGAGCTGTTTGGGACTA+TGG 0.236978 2_2:+4955292 Msa0232690:CDS
TTTCTTATTTAGAAGCTCTT+TGG 0.268782 2_2:-4953478 None:intergenic
TCAAATGAGAAGCTCTATAA+AGG 0.280877 2_2:+4953990 Msa0232690:CDS
GTAGTTCAATAATGGTTGTT+AGG 0.295009 2_2:+4953136 Msa0232690:exon
AAGATTTGGGATTCAGTTCT+TGG 0.304088 2_2:+4956416 Msa0232690:three_prime_UTR
AGTTCATCAATTTCCTCAAT+TGG 0.304511 2_2:-4954900 None:intergenic
AGGATTCTGCGTTAAGATTT+GGG 0.318411 2_2:+4956403 Msa0232690:three_prime_UTR
TGCAAATCCAGTACCAGTTC+TGG 0.327369 2_2:+4955172 Msa0232690:CDS
CAAATACAGTGTTTCACTTT+GGG 0.336751 2_2:+4952506 Msa0232690:five_prime_UTR
ATGCAGAACAAAATCGAAAA+AGG 0.349065 2_2:+4953546 Msa0232690:CDS
CCTTCTGTCAATTTATGGAT+TGG 0.349385 2_2:+4955205 Msa0232690:CDS
TCATAATCAAAACATCAAAA+TGG 0.358355 2_2:-4956620 None:intergenic
CTTCCATCAATGGCAGGACT+TGG 0.360593 2_2:-4954861 None:intergenic
TCGGTCACACTGCTTCATTT+TGG 0.361776 2_2:-4953949 None:intergenic
TCATTGTCAACTTTGAATTT+GGG 0.364609 2_2:+4953237 Msa0232690:CDS
TCATCAAAAGGCCTCTTTGC+TGG 0.367743 2_2:-4953315 None:intergenic
AGATATCTGTAGAGCTGTTT+GGG 0.367948 2_2:+4955285 Msa0232690:CDS
AAGATATCTGTAGAGCTGTT+TGG 0.368664 2_2:+4955284 Msa0232690:CDS
GCTGTTTGGGACTATGGGTT+TGG 0.369030 2_2:+4955298 Msa0232690:CDS
CCCCATCATTCCACAGTGTA+AGG 0.370588 2_2:+4955007 Msa0232690:CDS
CAACCAAGTCCTGCCATTGA+TGG 0.374788 2_2:+4954858 Msa0232690:CDS
AGAAGAGCATACTCGGATTA+AGG 0.374809 2_2:+4953403 Msa0232690:CDS
TTTGAAGAGGAAATTGAATC+AGG 0.376038 2_2:+4954719 Msa0232690:CDS
TCTGCATTTCCTCGTAATAT+TGG 0.377971 2_2:-4953530 None:intergenic
TTCTCAGACTAGCAATTTCC+TGG 0.380055 2_2:-4956230 None:intergenic
TGGGATTCAGTTCTTGGATG+TGG 0.380091 2_2:+4956422 Msa0232690:three_prime_UTR
GAGAGAAAGGCGAGTAGCTT+TGG 0.383992 2_2:+4956337 Msa0232690:exon
CCCTTACACTGTGGAATGAT+GGG 0.386689 2_2:-4955008 None:intergenic
GTTCATCAATTTCCTCAATT+GGG 0.386900 2_2:-4954899 None:intergenic
TGATTTGTGTAGTTCAATAA+TGG 0.390713 2_2:+4953128 Msa0232690:exon
TCCCTTACACTGTGGAATGA+TGG 0.397453 2_2:-4955009 None:intergenic
AGGACTTTGAAAGCCGAGTT+AGG 0.398394 2_2:+4954546 Msa0232690:CDS
AGGACTTTGAAAGCCGAGTT+AGG 0.398394 2_2:+4954588 Msa0232690:CDS
GACTTTGAAAGCCGAGTAAA+GGG 0.402680 2_2:+4954422 Msa0232690:CDS
CAGTGTGACCGACGACTAGA+AGG 0.410719 2_2:+4953960 Msa0232690:CDS
CCAAATACAGTGTTTCACTT+TGG 0.420774 2_2:+4952505 Msa0232690:five_prime_UTR
TAGTTCAATAATGGTTGTTA+GGG 0.427649 2_2:+4953137 Msa0232690:exon
GTAGAGCTGTTTGGGACTAT+GGG 0.430125 2_2:+4955293 Msa0232690:CDS
AAGGCGAGTAGCTTTGGTAT+TGG 0.430765 2_2:+4956343 Msa0232690:exon
TCAATCAGTTGATCTATTGC+GGG 0.431042 2_2:+4956034 Msa0232690:CDS
CTCTTTGCTGGCAGTCTAGT+AGG 0.431702 2_2:-4953303 None:intergenic
AACCTTGATTTCAATGGAGT+TGG 0.432488 2_2:-4956105 None:intergenic
TTGATTTGAGTCCCTTCAGT+TGG 0.434408 2_2:-4954181 None:intergenic
GATATTTGAAAGACGGATGA+AGG 0.437782 2_2:+4954211 Msa0232690:CDS
CTGTTTCAAACTTCGTCACT+CGG 0.440261 2_2:-4954475 None:intergenic
AACCAACTCCATTGAAATCA+AGG 0.448934 2_2:+4956103 Msa0232690:CDS
TCAAAGACAAGTAGATGATC+TGG 0.452367 2_2:+4954133 Msa0232690:CDS
GAAATATTGAGATAACATTA+CGG 0.455477 2_2:+4956702 Msa0232690:three_prime_UTR
TTTGCAGTTTCTCCCAATTG+AGG 0.456677 2_2:+4954887 Msa0232690:CDS
CTGATTTGAACTTCCTCACT+CGG 0.460825 2_2:-4954517 None:intergenic
GCCTTTACTATTGCTATCCA+TGG 0.462841 2_2:-4953673 None:intergenic
GTAGCTTTGGTATTGGAAGT+AGG 0.465926 2_2:+4956350 Msa0232690:exon
TAATCAAACCCTCGATGAAA+CGG 0.467409 2_2:-4953602 None:intergenic
TACTTACCTTTGTGTGGCTG+AGG 0.468990 2_2:-4952613 None:intergenic
ACTGTGGAATGATGGGGTTC+TGG 0.470582 2_2:-4955001 None:intergenic
GTAATGTCAGAGAGGAGAAC+TGG 0.471534 2_2:+4953855 Msa0232690:CDS
GTTGTCTCATTTACAAACTT+CGG 0.474404 2_2:-4956380 None:intergenic
AGGATGACAGTAATGAGAAC+TGG 0.474459 2_2:+4956547 Msa0232690:three_prime_UTR
GTAGTAAATCTATTGAGAAT+AGG 0.474756 2_2:+4953280 Msa0232690:CDS
GCAAATCCAGTACCAGTTCT+GGG 0.480024 2_2:+4955173 Msa0232690:CDS
GCAGGAGGAATTCGAAAGTC+AGG 0.484190 2_2:+4954037 Msa0232690:CDS
GCAATTTGAAAGCCAGGAAA+AGG 0.485355 2_2:+4954085 Msa0232690:CDS
CTCATGAAGAAGAGCATACT+CGG 0.485463 2_2:+4953396 Msa0232690:CDS
CAAATGAGAAGCTCTATAAA+GGG 0.486869 2_2:+4953991 Msa0232690:CDS
CTGCAAACTTCTTCCATCAA+TGG 0.487566 2_2:-4954871 None:intergenic
CTGATTCAAACTTCCTAACT+CGG 0.487972 2_2:-4954601 None:intergenic
GCTTGTTCAAATGAAGGACT+TGG 0.488868 2_2:+4953881 Msa0232690:CDS
AAGTAGGAATGAAATGAGAA+AGG 0.489576 2_2:-4952576 None:intergenic
CTGATTCAAACTTCCTAACT+CGG 0.490086 2_2:-4954559 None:intergenic
AAAGAAGCAATTTGAAAGCC+AGG 0.492303 2_2:+4954079 Msa0232690:CDS
GAAGTTTGAATCAGTAGAGA+AGG 0.504108 2_2:+4954568 Msa0232690:CDS
TTGATTCAAACCCCTTTACT+CGG 0.505676 2_2:-4954433 None:intergenic
AAGTTTGTAAATGAGACAAC+AGG 0.507654 2_2:+4956383 Msa0232690:three_prime_UTR
GGGATTCAGTTCTTGGATGT+GGG 0.508374 2_2:+4956423 Msa0232690:three_prime_UTR
TAGCTTTGGTATTGGAAGTA+GGG 0.508548 2_2:+4956351 Msa0232690:exon
GAAGTTTGAATCAGCAGAGA+AGG 0.509904 2_2:+4954610 Msa0232690:CDS
GGACTTTGAAAGCCGAGTAA+AGG 0.510913 2_2:+4954421 Msa0232690:CDS
TAAAGGCCGTGGAAGATTGA+AGG 0.511019 2_2:+4953689 Msa0232690:CDS
ATCAATCAGTTGATCTATTG+CGG 0.512272 2_2:+4956033 Msa0232690:CDS
GGAGAGTGAACTAAGAAAGT+AGG 0.514540 2_2:-4952592 None:intergenic
GAAGTTCAAATCAGTAGAGA+AGG 0.514891 2_2:+4954526 Msa0232690:CDS
GTTAATAAAATCATTTGAAG+AGG 0.515437 2_2:+4954706 Msa0232690:CDS
TTGATTCAAACTTCTTCACT+CGG 0.515510 2_2:-4954643 None:intergenic
TCAAAATTGAAGCAATATGA+AGG 0.517246 2_2:+4954326 Msa0232690:CDS
GCGCATGTCCGTTTCATCGA+GGG 0.520061 2_2:+4953594 Msa0232690:CDS
TCAATATTTCAAAGCAATTG+AGG 0.523088 2_2:-4956689 None:intergenic
GAGGAAATGCTGTCATCAAA+AGG 0.524403 2_2:-4953327 None:intergenic
CTTGAGCTAAATAGAGAGAA+AGG 0.526158 2_2:+4956324 Msa0232690:CDS
ATCCCTTGAGAGAGATATTG+AGG 0.530827 2_2:+4953449 Msa0232690:CDS
CAGCATTTCCTCGCAAACTT+CGG 0.531960 2_2:+4953338 Msa0232690:CDS
TAAAAGAACTAATGTCAGAA+AGG 0.536181 2_2:+4953817 Msa0232690:CDS
AAAGGTGTCGGAGTCAAAGC+AGG 0.537649 2_2:+4954019 Msa0232690:CDS
GAAGTTTGAATCAAAAGAAG+AGG 0.538867 2_2:+4954652 Msa0232690:CDS
TGGTCAGCTTGTTCAAATGA+AGG 0.542980 2_2:+4953875 Msa0232690:CDS
ATTCAACCCCTTCTAGTCGT+CGG 0.546192 2_2:-4953968 None:intergenic
TTGATTCGAAGTCCTTCACC+TGG 0.546907 2_2:-4954307 None:intergenic
CTTAAACTGAAAGCTTACAT+TGG 0.548401 2_2:+4955140 Msa0232690:CDS
AGAGTTTAAATCACAAGAGA+AGG 0.548434 2_2:+4954400 Msa0232690:CDS
TCAAAGAGAGAGGAATTTGA+AGG 0.551665 2_2:+4954368 Msa0232690:CDS
GACAATGACTTTGCTCTCAA+AGG 0.551969 2_2:-4953222 None:intergenic
ACTCCTCAATATCTCTCTCA+AGG 0.552126 2_2:-4953452 None:intergenic
TCAGGACAAGGCAAGAGACC+AGG 0.554657 2_2:+4956212 Msa0232690:intron
AAACTTCTTCCATCAATGGC+AGG 0.555447 2_2:-4954867 None:intergenic
ACAATTTACTTACCTTTGTG+TGG 0.555870 2_2:-4952619 None:intergenic
AGATAACAATATTGAATCTG+CGG 0.559408 2_2:+4956272 Msa0232690:CDS
AAGAGAAGATATTTGAAAGA+CGG 0.560688 2_2:+4954204 Msa0232690:CDS
TCAACGGAAAAGCAATTTGA+AGG 0.560873 2_2:+4953906 Msa0232690:CDS
ATTGCTTCTTCTCGCACATG+AGG 0.561232 2_2:-4955107 None:intergenic
GCAATATGAAGGACAAGTGA+AGG 0.566613 2_2:+4954337 Msa0232690:CDS
GAAAGCCAGGAAAAGGTGCT+CGG 0.567009 2_2:+4954092 Msa0232690:CDS
AAGTGAAACACTGTATTTGG+TGG 0.570485 2_2:-4952502 None:intergenic
TCAGGAAATTAAGACGAGAA+AGG 0.571467 2_2:+4953755 Msa0232690:CDS
GAAGTTTGAAACAGTAGAGA+AGG 0.571865 2_2:+4954484 Msa0232690:CDS
GGAATTCGAAAGTCAGGTGA+AGG 0.576331 2_2:+4954043 Msa0232690:CDS
GTGATTTAAACTCTTCCACT+CGG 0.576446 2_2:-4954391 None:intergenic
GATAGCAATAGTAAAGGCCG+TGG 0.579238 2_2:+4953678 Msa0232690:CDS
CGGATTAAGGAGCGTTGAGG+AGG 0.584542 2_2:+4953416 Msa0232690:CDS
TTTCTGCCCAGGATTTGAAG+TGG 0.585302 2_2:+4953105 Msa0232690:intron
GAAGGAGTTGAAGTCAAAAG+AGG 0.587532 2_2:+4954229 Msa0232690:CDS
CGCACATGAGGTTTAACATG+TGG 0.590019 2_2:-4955095 None:intergenic
CCAATCCATAAATTGACAGA+AGG 0.591953 2_2:-4955205 None:intergenic
GGAGTTACAATCAAAGAGAG+AGG 0.595701 2_2:+4954358 Msa0232690:CDS
GCCATGGATAGCAATAGTAA+AGG 0.596670 2_2:+4953672 Msa0232690:CDS
GAGACAACTTCTTACAGCCA+TGG 0.598031 2_2:+4953656 Msa0232690:CDS
CCCATCATTCCACAGTGTAA+GGG 0.604052 2_2:+4955008 Msa0232690:CDS
AATGAAGGACTTGGAGTCAA+CGG 0.606158 2_2:+4953890 Msa0232690:CDS
CCTTACACTGTGGAATGATG+GGG 0.610080 2_2:-4955007 None:intergenic
CTGATCGAGACACAAGGGGA+AGG 0.618054 2_2:+4956527 Msa0232690:three_prime_UTR
TAAAGGGCAGACAAAGGTGT+CGG 0.621702 2_2:+4954007 Msa0232690:CDS
CTCCTCAATATCTCTCTCAA+GGG 0.622421 2_2:-4953451 None:intergenic
TAGATTCATTTGTTATAACA+CGG 0.623887 2_2:+4956001 Msa0232690:CDS
AGTGTGACCGACGACTAGAA+GGG 0.627275 2_2:+4953961 Msa0232690:CDS
TTTATCTGATCGAGACACAA+GGG 0.627670 2_2:+4956522 Msa0232690:three_prime_UTR
ATTTATCTGATCGAGACACA+AGG 0.627865 2_2:+4956521 Msa0232690:three_prime_UTR
GAGGAACTTGAGCTAAGAGA+CGG 0.629178 2_2:+4954671 Msa0232690:CDS
AGAGGAATTTGAAGGCCGAG+TGG 0.631001 2_2:+4954376 Msa0232690:CDS
GCTCTATAAAGGGCAGACAA+AGG 0.633152 2_2:+4954001 Msa0232690:CDS
AGATGATCTGGAATCATGCA+AGG 0.638630 2_2:+4954145 Msa0232690:CDS
CACTCTCCTCAGCCACACAA+AGG 0.642678 2_2:+4952607 Msa0232690:five_prime_UTR
ACGGAAAAGCAATTTGAAGG+TGG 0.647692 2_2:+4953909 Msa0232690:CDS
AGCGCATGTCCGTTTCATCG+AGG 0.649306 2_2:+4953593 Msa0232690:CDS
CAAATCCAGTACCAGTTCTG+GGG 0.656039 2_2:+4955174 Msa0232690:CDS
GTGTGACCGACGACTAGAAG+GGG 0.661302 2_2:+4953962 Msa0232690:CDS
CATCAAAATGGTACAATACG+AGG 0.667651 2_2:-4956608 None:intergenic
CTACTAGACTGCCAGCAAAG+AGG 0.670048 2_2:+4953304 Msa0232690:CDS
CTTTGGTATTGGAAGTAGGG+CGG 0.670313 2_2:+4956354 Msa0232690:three_prime_UTR
TACTAAATGTAATGTCAGAG+AGG 0.673630 2_2:+4953847 Msa0232690:CDS
AGGACTTTGAAATCCGAGTG+AGG 0.681019 2_2:+4954504 Msa0232690:CDS
ACTTTGAAAGCCGAGTAAAG+GGG 0.687533 2_2:+4954423 Msa0232690:CDS
ACTCGGATTAAGGAGCGTTG+AGG 0.688313 2_2:+4953413 Msa0232690:CDS
GGTGTCGGAGTCAAAGCAGG+AGG 0.695615 2_2:+4954022 Msa0232690:CDS
TTATCTGATCGAGACACAAG+GGG 0.696466 2_2:+4956523 Msa0232690:three_prime_UTR
ATTGATGAACTTGAGTCACA+CGG 0.701580 2_2:+4954912 Msa0232690:CDS
ACGAACTAACCAATATTACG+AGG 0.703439 2_2:+4953521 Msa0232690:CDS
GTTGTTCGAGCAGATCAATG+TGG 0.704073 2_2:-4955058 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTAAATGATAAAAAAAAAAA+AGG - chr2_2:4952671-4952690 None:intergenic 10.0%
!! AACATTAAAAATTTATTGAA+TGG - chr2_2:4952714-4952733 None:intergenic 10.0%
!! ATCAAATGTATAAATATATT+TGG + chr2_2:4955454-4955473 Msa0232690:intron 10.0%
!! TTATAAAAAAGTTAGCATAT+TGG + chr2_2:4955600-4955619 Msa0232690:intron 15.0%
!! ATAACATCTCAAAATAAATT+GGG - chr2_2:4955886-4955905 None:intergenic 15.0%
!! AATAACATCTCAAAATAAAT+TGG - chr2_2:4955887-4955906 None:intergenic 15.0%
!! ATTATTTATAAATAACATGC+AGG - chr2_2:4956180-4956199 None:intergenic 15.0%
!!! AATAATTTCAATTTGAAATC+AGG + chr2_2:4956194-4956213 Msa0232690:intron 15.0%
!!! GATAGATAGTATTATAATTT+TGG + chr2_2:4956448-4956467 Msa0232690:three_prime_UTR 15.0%
!!! ATAGTATTATAATTTTGGAA+TGG + chr2_2:4956453-4956472 Msa0232690:three_prime_UTR 15.0%
!!! TTTTATCATTTACATTTCCA+TGG + chr2_2:4952678-4952697 Msa0232690:intron 20.0%
!! ATTTGCAGCTTATATATAAA+CGG - chr2_2:4953010-4953029 None:intergenic 20.0%
!!! ATTAAGTTGTTTATCCAAAA+AGG + chr2_2:4953033-4953052 Msa0232690:intron 20.0%
!!! TTAAGTTGTTTATCCAAAAA+GGG + chr2_2:4953034-4953053 Msa0232690:intron 20.0%
!! GTTAATAAAATCATTTGAAG+AGG + chr2_2:4954706-4954725 Msa0232690:CDS 20.0%
!!! TATCTAAAACATCTTTTGAT+GGG - chr2_2:4954977-4954996 None:intergenic 20.0%
!!! ATATCTAAAACATCTTTTGA+TGG - chr2_2:4954978-4954997 None:intergenic 20.0%
!!! ATGAAGTTTTAAAGCTTTTT+GGG + chr2_2:4955246-4955265 Msa0232690:CDS 20.0%
!!! ACAAATCAGAATTAACTTTT+GGG - chr2_2:4955581-4955600 None:intergenic 20.0%
!!! AACAAATCAGAATTAACTTT+TGG - chr2_2:4955582-4955601 None:intergenic 20.0%
!! ATATATCTTTCTAATTCAAG+TGG + chr2_2:4955655-4955674 Msa0232690:intron 20.0%
!! TAACATCTCAAAATAAATTG+GGG - chr2_2:4955885-4955904 None:intergenic 20.0%
!!! TAGATTCATTTGTTATAACA+CGG + chr2_2:4956001-4956020 Msa0232690:CDS 20.0%
!! AAAAAAAACCTTGATTTCAA+TGG - chr2_2:4956114-4956133 None:intergenic 20.0%
!! TCATAATCAAAACATCAAAA+TGG - chr2_2:4956623-4956642 None:intergenic 20.0%
!! GAAATATTGAGATAACATTA+CGG + chr2_2:4956702-4956721 Msa0232690:three_prime_UTR 20.0%
!! TGATTTGTGTAGTTCAATAA+TGG + chr2_2:4953128-4953147 Msa0232690:exon 25.0%
! TAGTTCAATAATGGTTGTTA+GGG + chr2_2:4953137-4953156 Msa0232690:exon 25.0%
! TCATTGTCAACTTTGAATTT+GGG + chr2_2:4953237-4953256 Msa0232690:CDS 25.0%
! GTAGTAAATCTATTGAGAAT+AGG + chr2_2:4953280-4953299 Msa0232690:CDS 25.0%
!! TTTCTTATTTAGAAGCTCTT+TGG - chr2_2:4953481-4953500 None:intergenic 25.0%
! TAAAAGAACTAATGTCAGAA+AGG + chr2_2:4953817-4953836 Msa0232690:CDS 25.0%
! AAGAGAAGATATTTGAAAGA+CGG + chr2_2:4954204-4954223 Msa0232690:CDS 25.0%
! TCAAAATTGAAGCAATATGA+AGG + chr2_2:4954326-4954345 Msa0232690:CDS 25.0%
!!! GATGAAGTTTTAAAGCTTTT+TGG + chr2_2:4955245-4955264 Msa0232690:CDS 25.0%
! ACTAAGAAATTGAACTGATA+TGG + chr2_2:4955508-4955527 Msa0232690:intron 25.0%
! AAATTGAACTGATATGGAAT+TGG + chr2_2:4955514-4955533 Msa0232690:intron 25.0%
!! AAATCAGAATTAACTTTTGG+GGG - chr2_2:4955579-4955598 None:intergenic 25.0%
!! CAAATCAGAATTAACTTTTG+GGG - chr2_2:4955580-4955599 None:intergenic 25.0%
! TGGTTAAAGAGTTTCAATTA+AGG + chr2_2:4955675-4955694 Msa0232690:intron 25.0%
!!! CATATGTGCTGTTTTTTTAA+TGG + chr2_2:4955838-4955857 Msa0232690:intron 25.0%
!!! TAGATCAACTGATTGATTTT+TGG - chr2_2:4956030-4956049 None:intergenic 25.0%
! AGATAACAATATTGAATCTG+CGG + chr2_2:4956272-4956291 Msa0232690:CDS 25.0%
! AACAAACTGATGTAAAAATC+TGG + chr2_2:4956578-4956597 Msa0232690:three_prime_UTR 25.0%
! TCAATATTTCAAAGCAATTG+AGG - chr2_2:4956692-4956711 None:intergenic 25.0%
CAAATACAGTGTTTCACTTT+GGG + chr2_2:4952506-4952525 Msa0232690:five_prime_UTR 30.0%
AAGTAGGAATGAAATGAGAA+AGG - chr2_2:4952579-4952598 None:intergenic 30.0%
ACAATTTACTTACCTTTGTG+TGG - chr2_2:4952622-4952641 None:intergenic 30.0%
TATCAATCACAAATCAGCTA+GGG - chr2_2:4952768-4952787 None:intergenic 30.0%
! ATTAGTCTAAGAAAACATGG+GGG - chr2_2:4952797-4952816 None:intergenic 30.0%
! CATTAGTCTAAGAAAACATG+GGG - chr2_2:4952798-4952817 None:intergenic 30.0%
! GCATTAGTCTAAGAAAACAT+GGG - chr2_2:4952799-4952818 None:intergenic 30.0%
! TGCATTAGTCTAAGAAAACA+TGG - chr2_2:4952800-4952819 None:intergenic 30.0%
CTTATAACATGAGATCTGTT+TGG + chr2_2:4952834-4952853 Msa0232690:intron 30.0%
!! AATAGTATCAAAGGTGCTTA+TGG - chr2_2:4952887-4952906 None:intergenic 30.0%
! TCAACTATTAAGACCCTTTT+TGG - chr2_2:4953050-4953069 None:intergenic 30.0%
GTAGTTCAATAATGGTTGTT+AGG + chr2_2:4953136-4953155 Msa0232690:exon 30.0%
GTCATTGTCAACTTTGAATT+TGG + chr2_2:4953236-4953255 Msa0232690:CDS 30.0%
ATGCAGAACAAAATCGAAAA+AGG + chr2_2:4953546-4953565 Msa0232690:CDS 30.0%
AAAAATTGCTGAATGTACTC+AGG + chr2_2:4953737-4953756 Msa0232690:CDS 30.0%
TACTAAATGTAATGTCAGAG+AGG + chr2_2:4953847-4953866 Msa0232690:CDS 30.0%
TCAAATGAGAAGCTCTATAA+AGG + chr2_2:4953990-4954009 Msa0232690:CDS 30.0%
CAAATGAGAAGCTCTATAAA+GGG + chr2_2:4953991-4954010 Msa0232690:CDS 30.0%
!! TTAAGAGAGTGTGATTTTGA+AGG + chr2_2:4954284-4954303 Msa0232690:CDS 30.0%
AGAGTTTAAATCACAAGAGA+AGG + chr2_2:4954400-4954419 Msa0232690:CDS 30.0%
TTGATTCAAACTTCTTCACT+CGG - chr2_2:4954646-4954665 None:intergenic 30.0%
GAAGTTTGAATCAAAAGAAG+AGG + chr2_2:4954652-4954671 Msa0232690:CDS 30.0%
TTTGAAGAGGAAATTGAATC+AGG + chr2_2:4954719-4954738 Msa0232690:CDS 30.0%
GATGAAATTGCATAATTAGC+TGG - chr2_2:4954825-4954844 None:intergenic 30.0%
GTTCATCAATTTCCTCAATT+GGG - chr2_2:4954902-4954921 None:intergenic 30.0%
AGTTCATCAATTTCCTCAAT+TGG - chr2_2:4954903-4954922 None:intergenic 30.0%
CTTAAACTGAAAGCTTACAT+TGG + chr2_2:4955140-4955159 Msa0232690:CDS 30.0%
CAATGTGAAGAAAGACAAAA+AGG - chr2_2:4955380-4955399 None:intergenic 30.0%
TTCTTATCACTTCTAAGTGT+AGG + chr2_2:4955416-4955435 Msa0232690:intron 30.0%
TCAATTAAGGACGAATCATT+TGG + chr2_2:4955688-4955707 Msa0232690:intron 30.0%
CAATTAAGGACGAATCATTT+GGG + chr2_2:4955689-4955708 Msa0232690:intron 30.0%
ATATAAGAAAAGCTTGACCA+AGG - chr2_2:4955747-4955766 None:intergenic 30.0%
! GTCAAGCTTTTCTTATATCT+TGG + chr2_2:4955749-4955768 Msa0232690:intron 30.0%
!!! TTTTTTTGGTCTTAACACTC+CGG - chr2_2:4955813-4955832 None:intergenic 30.0%
!!! GCACATATGCATAGTTTTTT+TGG - chr2_2:4955827-4955846 None:intergenic 30.0%
!!! GCTGTTTTTTTAATGGTTTG+AGG + chr2_2:4955845-4955864 Msa0232690:intron 30.0%
ACTTCATCAAACTAACATAC+GGG - chr2_2:4955913-4955932 None:intergenic 30.0%
CACTTCATCAAACTAACATA+CGG - chr2_2:4955914-4955933 None:intergenic 30.0%
!! ATCAACTGATTGATTTTTGG+TGG - chr2_2:4956027-4956046 None:intergenic 30.0%
ATCAATCAGTTGATCTATTG+CGG + chr2_2:4956033-4956052 Msa0232690:CDS 30.0%
! TTCAATTTGAAATCAGGACA+AGG + chr2_2:4956200-4956219 Msa0232690:intron 30.0%
GTTGTCTCATTTACAAACTT+CGG - chr2_2:4956383-4956402 None:intergenic 30.0%
AAGTTTGTAAATGAGACAAC+AGG + chr2_2:4956383-4956402 Msa0232690:three_prime_UTR 30.0%
!!! GCTAACTCTTTTGTTTTTGT+TGG - chr2_2:4956670-4956689 None:intergenic 30.0%
CCAAAGTGAAACACTGTATT+TGG - chr2_2:4952508-4952527 None:intergenic 35.0%
CCAAATACAGTGTTTCACTT+TGG + chr2_2:4952505-4952524 Msa0232690:five_prime_UTR 35.0%
!!! TGAATGGATGCTATTTTCCA+TGG - chr2_2:4952698-4952717 None:intergenic 35.0%
CTATCAATCACAAATCAGCT+AGG - chr2_2:4952769-4952788 None:intergenic 35.0%
GTTCGCTCAAATAGTATCAA+AGG - chr2_2:4952896-4952915 None:intergenic 35.0%
TTTGAGCGAACTTATGAAGT+TGG + chr2_2:4952905-4952924 Msa0232690:intron 35.0%
!!! TCTTTTTTCTTTTTCTGCCC+AGG + chr2_2:4953094-4953113 Msa0232690:intron 35.0%
AAATCACCACTTCAAATCCT+GGG - chr2_2:4953114-4953133 None:intergenic 35.0%
!!! TTTTTTAACCGAAGTTTGCG+AGG - chr2_2:4953349-4953368 None:intergenic 35.0%
ACGAACTAACCAATATTACG+AGG + chr2_2:4953521-4953540 Msa0232690:CDS 35.0%
TCTGCATTTCCTCGTAATAT+TGG - chr2_2:4953533-4953552 None:intergenic 35.0%
TAATCAAACCCTCGATGAAA+CGG - chr2_2:4953605-4953624 None:intergenic 35.0%
! CTTTTTCCTTCAATCTTCCA+CGG - chr2_2:4953698-4953717 None:intergenic 35.0%
TCAGGAAATTAAGACGAGAA+AGG + chr2_2:4953755-4953774 Msa0232690:CDS 35.0%
TCAACGGAAAAGCAATTTGA+AGG + chr2_2:4953906-4953925 Msa0232690:CDS 35.0%
AAAGAAGCAATTTGAAAGCC+AGG + chr2_2:4954079-4954098 Msa0232690:CDS 35.0%
TCAAAGACAAGTAGATGATC+TGG + chr2_2:4954133-4954152 Msa0232690:CDS 35.0%
!! CATTTTGAAAGCCAACTGAA+GGG + chr2_2:4954170-4954189 Msa0232690:CDS 35.0%
GATATTTGAAAGACGGATGA+AGG + chr2_2:4954211-4954230 Msa0232690:CDS 35.0%
TCAAAGAGAGAGGAATTTGA+AGG + chr2_2:4954368-4954387 Msa0232690:CDS 35.0%
GTGATTTAAACTCTTCCACT+CGG - chr2_2:4954394-4954413 None:intergenic 35.0%
TTGATTCAAACCCCTTTACT+CGG - chr2_2:4954436-4954455 None:intergenic 35.0%
GGGGTTTGAATCAAAAAAGA+AGG + chr2_2:4954442-4954461 Msa0232690:CDS 35.0%
! GAAGTTTGAAACAGTAGAGA+AGG + chr2_2:4954484-4954503 Msa0232690:CDS 35.0%
GAAGTTCAAATCAGTAGAGA+AGG + chr2_2:4954526-4954545 Msa0232690:CDS 35.0%
CTGATTCAAACTTCCTAACT+CGG - chr2_2:4954562-4954581 None:intergenic 35.0%
GAAGTTTGAATCAGTAGAGA+AGG + chr2_2:4954568-4954587 Msa0232690:CDS 35.0%
CTGATTCAAACTTCCTAACT+CGG - chr2_2:4954562-4954581 None:intergenic 35.0%
ATTGATGAACTTGAGTCACA+CGG + chr2_2:4954912-4954931 Msa0232690:CDS 35.0%
TTGCTCCTTCTGTCAATTTA+TGG + chr2_2:4955200-4955219 Msa0232690:CDS 35.0%
CCAATCCATAAATTGACAGA+AGG - chr2_2:4955208-4955227 None:intergenic 35.0%
CCTTCTGTCAATTTATGGAT+TGG + chr2_2:4955205-4955224 Msa0232690:CDS 35.0%
AAGATATCTGTAGAGCTGTT+TGG + chr2_2:4955284-4955303 Msa0232690:CDS 35.0%
AGATATCTGTAGAGCTGTTT+GGG + chr2_2:4955285-4955304 Msa0232690:CDS 35.0%
TAAGTGTAGGTTACATCAAG+TGG + chr2_2:4955429-4955448 Msa0232690:intron 35.0%
! TACCAAAACCCTTTTCTCTT+AGG + chr2_2:4955785-4955804 Msa0232690:intron 35.0%
! TTCCTAAGAGAAAAGGGTTT+TGG - chr2_2:4955790-4955809 None:intergenic 35.0%
GATTCTGAACTAAATCTGCA+AGG - chr2_2:4955950-4955969 None:intergenic 35.0%
TCAATCAGTTGATCTATTGC+GGG + chr2_2:4956034-4956053 Msa0232690:CDS 35.0%
! ATTTTATGAGAGCAGTTGCA+AGG + chr2_2:4956079-4956098 Msa0232690:CDS 35.0%
AACCAACTCCATTGAAATCA+AGG + chr2_2:4956103-4956122 Msa0232690:CDS 35.0%
AACCTTGATTTCAATGGAGT+TGG - chr2_2:4956108-4956127 None:intergenic 35.0%
CTTGAGCTAAATAGAGAGAA+AGG + chr2_2:4956324-4956343 Msa0232690:CDS 35.0%
!! TAGCTTTGGTATTGGAAGTA+GGG + chr2_2:4956351-4956370 Msa0232690:exon 35.0%
AGGATTCTGCGTTAAGATTT+GGG + chr2_2:4956403-4956422 Msa0232690:three_prime_UTR 35.0%
! AAGATTTGGGATTCAGTTCT+TGG + chr2_2:4956416-4956435 Msa0232690:three_prime_UTR 35.0%
ATTTATCTGATCGAGACACA+AGG + chr2_2:4956521-4956540 Msa0232690:three_prime_UTR 35.0%
TTTATCTGATCGAGACACAA+GGG + chr2_2:4956522-4956541 Msa0232690:three_prime_UTR 35.0%
CATCAAAATGGTACAATACG+AGG - chr2_2:4956611-4956630 None:intergenic 35.0%
GGAGAGTGAACTAAGAAAGT+AGG - chr2_2:4952595-4952614 None:intergenic 40.0%
CAAATCACCACTTCAAATCC+TGG - chr2_2:4953115-4953134 None:intergenic 40.0%
GACAATGACTTTGCTCTCAA+AGG - chr2_2:4953225-4953244 None:intergenic 40.0%
GAGGAAATGCTGTCATCAAA+AGG - chr2_2:4953330-4953349 None:intergenic 40.0%
GCTCTTCTTCATGAGCTTAA+TGG - chr2_2:4953391-4953410 None:intergenic 40.0%
! CTCATGAAGAAGAGCATACT+CGG + chr2_2:4953396-4953415 Msa0232690:CDS 40.0%
! AGAAGAGCATACTCGGATTA+AGG + chr2_2:4953403-4953422 Msa0232690:CDS 40.0%
ATCCCTTGAGAGAGATATTG+AGG + chr2_2:4953449-4953468 Msa0232690:CDS 40.0%
CTCCTCAATATCTCTCTCAA+GGG - chr2_2:4953454-4953473 None:intergenic 40.0%
ACTCCTCAATATCTCTCTCA+AGG - chr2_2:4953455-4953474 None:intergenic 40.0%
GCCATGGATAGCAATAGTAA+AGG + chr2_2:4953672-4953691 Msa0232690:CDS 40.0%
GCCTTTACTATTGCTATCCA+TGG - chr2_2:4953676-4953695 None:intergenic 40.0%
! TGGTCAGCTTGTTCAAATGA+AGG + chr2_2:4953875-4953894 Msa0232690:CDS 40.0%
GCTTGTTCAAATGAAGGACT+TGG + chr2_2:4953881-4953900 Msa0232690:CDS 40.0%
AATGAAGGACTTGGAGTCAA+CGG + chr2_2:4953890-4953909 Msa0232690:CDS 40.0%
ACGGAAAAGCAATTTGAAGG+TGG + chr2_2:4953909-4953928 Msa0232690:CDS 40.0%
GCAATTTGAAAGCCAGGAAA+AGG + chr2_2:4954085-4954104 Msa0232690:CDS 40.0%
! AGATGATCTGGAATCATGCA+AGG + chr2_2:4954145-4954164 Msa0232690:CDS 40.0%
!! GCATTTTGAAAGCCAACTGA+AGG + chr2_2:4954169-4954188 Msa0232690:CDS 40.0%
! TTGATTTGAGTCCCTTCAGT+TGG - chr2_2:4954184-4954203 None:intergenic 40.0%
GAAGGAGTTGAAGTCAAAAG+AGG + chr2_2:4954229-4954248 Msa0232690:CDS 40.0%
! ACGTTTTCAAAGACGAGTGA+AGG + chr2_2:4954253-4954272 Msa0232690:CDS 40.0%
GCAATATGAAGGACAAGTGA+AGG + chr2_2:4954337-4954356 Msa0232690:CDS 40.0%
GGAGTTACAATCAAAGAGAG+AGG + chr2_2:4954358-4954377 Msa0232690:CDS 40.0%
! GACTTTGAAAGCCGAGTAAA+GGG + chr2_2:4954422-4954441 Msa0232690:CDS 40.0%
! ACTTTGAAAGCCGAGTAAAG+GGG + chr2_2:4954423-4954442 Msa0232690:CDS 40.0%
CTGTTTCAAACTTCGTCACT+CGG - chr2_2:4954478-4954497 None:intergenic 40.0%
! CTGATTTGAACTTCCTCACT+CGG - chr2_2:4954520-4954539 None:intergenic 40.0%
GAAGTTTGAATCAGCAGAGA+AGG + chr2_2:4954610-4954629 Msa0232690:CDS 40.0%
AAACTTCTTCCATCAATGGC+AGG - chr2_2:4954870-4954889 None:intergenic 40.0%
CTGCAAACTTCTTCCATCAA+TGG - chr2_2:4954874-4954893 None:intergenic 40.0%
TTTGCAGTTTCTCCCAATTG+AGG + chr2_2:4954887-4954906 Msa0232690:CDS 40.0%
! ACATTTTCTCCCTTACACTG+TGG - chr2_2:4955020-4955039 None:intergenic 40.0%
GGTTTGGCACATAAAGTTTC+TGG + chr2_2:4955314-4955333 Msa0232690:CDS 40.0%
ATCTTGGCAAGAACAATCCT+TGG + chr2_2:4955727-4955746 Msa0232690:intron 40.0%
CTTATATCTTGGTCGAACTC+TGG + chr2_2:4955760-4955779 Msa0232690:intron 40.0%
! AACCCTTTTCTCTTAGGAAC+CGG + chr2_2:4955791-4955810 Msa0232690:intron 40.0%
TTCTCAGACTAGCAATTTCC+TGG - chr2_2:4956233-4956252 None:intergenic 40.0%
!! GTAGCTTTGGTATTGGAAGT+AGG + chr2_2:4956350-4956369 Msa0232690:exon 40.0%
CAGGATTCTGCGTTAAGATT+TGG + chr2_2:4956402-4956421 Msa0232690:three_prime_UTR 40.0%
TTATCTGATCGAGACACAAG+GGG + chr2_2:4956523-4956542 Msa0232690:three_prime_UTR 40.0%
AGGATGACAGTAATGAGAAC+TGG + chr2_2:4956547-4956566 Msa0232690:three_prime_UTR 40.0%
TACTTACCTTTGTGTGGCTG+AGG - chr2_2:4952616-4952635 None:intergenic 45.0%
TTTCTGCCCAGGATTTGAAG+TGG + chr2_2:4953105-4953124 Msa0232690:intron 45.0%
TCATCAAAAGGCCTCTTTGC+TGG - chr2_2:4953318-4953337 None:intergenic 45.0%
CAGCATTTCCTCGCAAACTT+CGG + chr2_2:4953338-4953357 Msa0232690:CDS 45.0%
GAGACAACTTCTTACAGCCA+TGG + chr2_2:4953656-4953675 Msa0232690:CDS 45.0%
GATAGCAATAGTAAAGGCCG+TGG + chr2_2:4953678-4953697 Msa0232690:CDS 45.0%
TAAAGGCCGTGGAAGATTGA+AGG + chr2_2:4953689-4953708 Msa0232690:CDS 45.0%
GTAATGTCAGAGAGGAGAAC+TGG + chr2_2:4953855-4953874 Msa0232690:CDS 45.0%
! TCGGTCACACTGCTTCATTT+TGG - chr2_2:4953952-4953971 None:intergenic 45.0%
ATTCAACCCCTTCTAGTCGT+CGG - chr2_2:4953971-4953990 None:intergenic 45.0%
GCTCTATAAAGGGCAGACAA+AGG + chr2_2:4954001-4954020 Msa0232690:CDS 45.0%
! TAAAGGGCAGACAAAGGTGT+CGG + chr2_2:4954007-4954026 Msa0232690:CDS 45.0%
GGAATTCGAAAGTCAGGTGA+AGG + chr2_2:4954043-4954062 Msa0232690:CDS 45.0%
! TTAATCCGAGCACCTTTTCC+TGG - chr2_2:4954100-4954119 None:intergenic 45.0%
!! AGAGTGTGATTTTGAAGGCC+AGG + chr2_2:4954289-4954308 Msa0232690:CDS 45.0%
!! TGATTTTGAAGGCCAGGTGA+AGG + chr2_2:4954295-4954314 Msa0232690:CDS 45.0%
! TTGATTCGAAGTCCTTCACC+TGG - chr2_2:4954310-4954329 None:intergenic 45.0%
! GGACTTTGAAAGCCGAGTAA+AGG + chr2_2:4954421-4954440 Msa0232690:CDS 45.0%
!! AGGACTTTGAAATCCGAGTG+AGG + chr2_2:4954504-4954523 Msa0232690:CDS 45.0%
! AGGACTTTGAAAGCCGAGTT+AGG + chr2_2:4954546-4954565 Msa0232690:CDS 45.0%
! AGGACTTTGAAAGCCGAGTT+AGG + chr2_2:4954546-4954565 Msa0232690:CDS 45.0%
GAGGAACTTGAGCTAAGAGA+CGG + chr2_2:4954671-4954690 Msa0232690:CDS 45.0%
CCTTACACTGTGGAATGATG+GGG - chr2_2:4955010-4955029 None:intergenic 45.0%
CCCTTACACTGTGGAATGAT+GGG - chr2_2:4955011-4955030 None:intergenic 45.0%
CCCATCATTCCACAGTGTAA+GGG + chr2_2:4955008-4955027 Msa0232690:CDS 45.0%
TCCCTTACACTGTGGAATGA+TGG - chr2_2:4955012-4955031 None:intergenic 45.0%
GTTGTTCGAGCAGATCAATG+TGG - chr2_2:4955061-4955080 None:intergenic 45.0%
CGCACATGAGGTTTAACATG+TGG - chr2_2:4955098-4955117 None:intergenic 45.0%
ATTGCTTCTTCTCGCACATG+AGG - chr2_2:4955110-4955129 None:intergenic 45.0%
TGCAAATCCAGTACCAGTTC+TGG + chr2_2:4955172-4955191 Msa0232690:CDS 45.0%
GCAAATCCAGTACCAGTTCT+GGG + chr2_2:4955173-4955192 Msa0232690:CDS 45.0%
CAAATCCAGTACCAGTTCTG+GGG + chr2_2:4955174-4955193 Msa0232690:CDS 45.0%
AAAAACCCCAGAACTGGTAC+TGG - chr2_2:4955182-4955201 None:intergenic 45.0%
! TGTAGAGCTGTTTGGGACTA+TGG + chr2_2:4955292-4955311 Msa0232690:CDS 45.0%
! GTAGAGCTGTTTGGGACTAT+GGG + chr2_2:4955293-4955312 Msa0232690:CDS 45.0%
! GAGCATTCGAGTTCGAATCT+TGG + chr2_2:4955711-4955730 Msa0232690:intron 45.0%
CTCCGGTTCCTAAGAGAAAA+GGG - chr2_2:4955796-4955815 None:intergenic 45.0%
ACTCCGGTTCCTAAGAGAAA+AGG - chr2_2:4955797-4955816 None:intergenic 45.0%
GAACTAAATCTGCAAGGTGC+AGG - chr2_2:4955944-4955963 None:intergenic 45.0%
AACATGCAGGTGCATGAAGA+TGG - chr2_2:4956167-4956186 None:intergenic 45.0%
!! AAGGCGAGTAGCTTTGGTAT+TGG + chr2_2:4956343-4956362 Msa0232690:exon 45.0%
! CTTTGGTATTGGAAGTAGGG+CGG + chr2_2:4956354-4956373 Msa0232690:three_prime_UTR 45.0%
TGGGATTCAGTTCTTGGATG+TGG + chr2_2:4956422-4956441 Msa0232690:three_prime_UTR 45.0%
GGGATTCAGTTCTTGGATGT+GGG + chr2_2:4956423-4956442 Msa0232690:three_prime_UTR 45.0%
!! TAAATGATAAAAAAAAAAAA+GGG - chr2_2:4952670-4952689 None:intergenic 5.0%
CTCTTTGCTGGCAGTCTAGT+AGG - chr2_2:4953306-4953325 None:intergenic 50.0%
CTACTAGACTGCCAGCAAAG+AGG + chr2_2:4953304-4953323 Msa0232690:CDS 50.0%
!! ACTCGGATTAAGGAGCGTTG+AGG + chr2_2:4953413-4953432 Msa0232690:CDS 50.0%
AGTGTGACCGACGACTAGAA+GGG + chr2_2:4953961-4953980 Msa0232690:CDS 50.0%
!! AAAGGTGTCGGAGTCAAAGC+AGG + chr2_2:4954019-4954038 Msa0232690:CDS 50.0%
GCAGGAGGAATTCGAAAGTC+AGG + chr2_2:4954037-4954056 Msa0232690:CDS 50.0%
!! GAAAGCCAGGAAAAGGTGCT+CGG + chr2_2:4954092-4954111 Msa0232690:CDS 50.0%
AGAGGAATTTGAAGGCCGAG+TGG + chr2_2:4954376-4954395 Msa0232690:CDS 50.0%
CAACCAAGTCCTGCCATTGA+TGG + chr2_2:4954858-4954877 Msa0232690:CDS 50.0%
CTTCCATCAATGGCAGGACT+TGG - chr2_2:4954864-4954883 None:intergenic 50.0%
! ACTGTGGAATGATGGGGTTC+TGG - chr2_2:4955004-4955023 None:intergenic 50.0%
CCCCATCATTCCACAGTGTA+AGG + chr2_2:4955007-4955026 Msa0232690:CDS 50.0%
AGGAGCAAAAACCCCAGAAC+TGG - chr2_2:4955188-4955207 None:intergenic 50.0%
! GCTGTTTGGGACTATGGGTT+TGG + chr2_2:4955298-4955317 Msa0232690:CDS 50.0%
GAGAGAAAGGCGAGTAGCTT+TGG + chr2_2:4956337-4956356 Msa0232690:exon 50.0%
CACTCTCCTCAGCCACACAA+AGG + chr2_2:4952607-4952626 Msa0232690:five_prime_UTR 55.0%
!! CGGATTAAGGAGCGTTGAGG+AGG + chr2_2:4953416-4953435 Msa0232690:CDS 55.0%
AGCGCATGTCCGTTTCATCG+AGG + chr2_2:4953593-4953612 Msa0232690:CDS 55.0%
GCGCATGTCCGTTTCATCGA+GGG + chr2_2:4953594-4953613 Msa0232690:CDS 55.0%
CAGTGTGACCGACGACTAGA+AGG + chr2_2:4953960-4953979 Msa0232690:CDS 55.0%
GTGTGACCGACGACTAGAAG+GGG + chr2_2:4953962-4953981 Msa0232690:CDS 55.0%
TCAGGACAAGGCAAGAGACC+AGG + chr2_2:4956212-4956231 Msa0232690:intron 55.0%
CTGATCGAGACACAAGGGGA+AGG + chr2_2:4956527-4956546 Msa0232690:three_prime_UTR 55.0%
! GGTGTCGGAGTCAAAGCAGG+AGG + chr2_2:4954022-4954041 Msa0232690:CDS 60.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 4952504 4956733 4952504 ID=Msa0232690;Name=Msa0232690
chr2_2 mRNA 4952504 4956733 4952504 ID=Msa0232690-mRNA-1;Parent=Msa0232690;Name=Msa0232690-mRNA-1;_AED=0.11;_eAED=0.11;_QI=156|0.75|0.8|1|0.75|0.6|5|380|803
chr2_2 exon 4952504 4952628 4952504 ID=Msa0232690-mRNA-1:exon:2131;Parent=Msa0232690-mRNA-1
chr2_2 exon 4953116 4954740 4953116 ID=Msa0232690-mRNA-1:exon:2132;Parent=Msa0232690-mRNA-1
chr2_2 exon 4954826 4955335 4954826 ID=Msa0232690-mRNA-1:exon:2133;Parent=Msa0232690-mRNA-1
chr2_2 exon 4955955 4956124 4955955 ID=Msa0232690-mRNA-1:exon:2134;Parent=Msa0232690-mRNA-1
chr2_2 exon 4956216 4956733 4956216 ID=Msa0232690-mRNA-1:exon:2135;Parent=Msa0232690-mRNA-1
chr2_2 five_prime_UTR 4952504 4952628 4952504 ID=Msa0232690-mRNA-1:five_prime_utr;Parent=Msa0232690-mRNA-1
chr2_2 five_prime_UTR 4953116 4953146 4953116 ID=Msa0232690-mRNA-1:five_prime_utr;Parent=Msa0232690-mRNA-1
chr2_2 CDS 4953147 4954740 4953147 ID=Msa0232690-mRNA-1:cds;Parent=Msa0232690-mRNA-1
chr2_2 CDS 4954826 4955335 4954826 ID=Msa0232690-mRNA-1:cds;Parent=Msa0232690-mRNA-1
chr2_2 CDS 4955955 4956124 4955955 ID=Msa0232690-mRNA-1:cds;Parent=Msa0232690-mRNA-1
chr2_2 CDS 4956216 4956353 4956216 ID=Msa0232690-mRNA-1:cds;Parent=Msa0232690-mRNA-1
chr2_2 three_prime_UTR 4956354 4956733 4956354 ID=Msa0232690-mRNA-1:three_prime_utr;Parent=Msa0232690-mRNA-1
Gene Sequence

>Msa0232690

ATGGTTGTTAGGGCTTCTAAAAGAAAAAAGTTGCTGAATTTATATGACTCTAAGTCTGATGATGATTATGATGTACCTTTGAGAGCAAAGTCATTGTCAACTTTGAATTTGGGAGCAAGTAGTAGTAGTAGTAGTAGTAAATCTATTGAGAATAGGCCTACTAGACTGCCAGCAAAGAGGCCTTTTGATGACAGCATTTCCTCGCAAACTTCGGTTAAAAAATCAAAGTTGTCATCGTTTTCCATTAAGCTCATGAAGAAGAGCATACTCGGATTAAGGAGCGTTGAGGAGGAAAAATTGCAATCCCTTGAGAGAGATATTGAGGAGTGCTCCAAAGAGCTTCTAAATAAGAAAAAGCAAGCTAGTGATGTTAGACGAACTAACCAATATTACGAGGAAATGCAGAACAAAATCGAAAAAGGTGTCAAAGATCTTGCAGCAAACGAAGCGCATGTCCGTTTCATCGAGGGTTTGATTAAAAACAAAACACTTGAGCTCAAGAAAAATAAGAGACAACTTCTTACAGCCATGGATAGCAATAGTAAAGGCCGTGGAAGATTGAAGGAAAAAGAGCTAGAGACTCTTTCACAAAAAATTGCTGAATGTACTCAGGAAATTAAGACGAGAAAGGAAGAGCTTGATGCTTCGAAAATATCTGTTAATCATAAAATAAAAGAACTAATGTCAGAAAGGAGTAACATACTAAATGTAATGTCAGAGAGGAGAACTGGTCAGCTTGTTCAAATGAAGGACTTGGAGTCAACGGAAAAGCAATTTGAAGGTGGAGCTACAGAGTTTGATTCCAAAATGAAGCAGTGTGACCGACGACTAGAAGGGGTTGAATCAAATGAGAAGCTCTATAAAGGGCAGACAAAGGTGTCGGAGTCAAAGCAGGAGGAATTCGAAAGTCAGGTGAAGGAGCTTGAATCAAAAAAGAAGCAATTTGAAAGCCAGGAAAAGGTGCTCGGATTAAAAGAGAAGCTATTTCAAAGACAAGTAGATGATCTGGAATCATGCAAGGAGCATTTTGAAAGCCAACTGAAGGGACTCAAATCAAAAGAGAAGATATTTGAAAGACGGATGAAGGAGTTGAAGTCAAAAGAGGAACGTTTTCAAAGACGAGTGAAGGAGTTTGAATTAAGAGAGTGTGATTTTGAAGGCCAGGTGAAGGACTTCGAATCAAAATTGAAGCAATATGAAGGACAAGTGAAGGAGTTACAATCAAAGAGAGAGGAATTTGAAGGCCGAGTGGAAGAGTTTAAATCACAAGAGAAGGACTTTGAAAGCCGAGTAAAGGGGTTTGAATCAAAAAAGAAGGACTTTGAAAGCCGAGTGACGAAGTTTGAAACAGTAGAGAAGGACTTTGAAATCCGAGTGAGGAAGTTCAAATCAGTAGAGAAGGACTTTGAAAGCCGAGTTAGGAAGTTTGAATCAGTAGAGAAGGACTTTGAAAGCCGAGTTAGGAAGTTTGAATCAGCAGAGAAGGACTTTGAAAGCCGAGTGAAGAAGTTTGAATCAAAAGAAGAGGAACTTGAGCTAAGAGACGGTCAATATGAAACGTTAATAAAATCATTTGAAGAGGAAATTGAATCAGCTAATTATGCAATTTCATCTTTCACAGATGATCAACCAAGTCCTGCCATTGATGGAAGAAGTTTGCAGTTTCTCCCAATTGAGGAAATTGATGAACTTGAGTCACACGGCAATGACAGTCTAGCTAATCTGCTAGCATCATCATCAGACCCATCAAAAGATGTTTTAGATATAATCCAGAACCCCATCATTCCACAGTGTAAGGGAGAAAATGTTGTAATTATTGATGATCACCACATTGATCTGCTCGAACAACTGATGAGAATCTCACCACATGTTAAACCTCATGTGCGAGAAGAAGCAATGAAGCTAGCACTTAAACTGAAAGCTTACATTGGAGAAAATACTGCAAATCCAGTACCAGTTCTGGGGTTTTTGCTCCTTCTGTCAATTTATGGATTGGTTTCTTCTTTCGATGAAGATGAAGTTTTAAAGCTTTTTGGGTTTGCTGCTCAACACAAGATATCTGTAGAGCTGTTTGGGACTATGGGTTTGGCACATAAAGTTTCTGATTTAGTTCAGAATCTTATCATGAAGCAGCAATATATTGAAGCTGTTAGATTCATTTGTTATAACACGGCCACCAAAAATCAATCAGTTGATCTATTGCGGGAACATGTGCAGAATGCAAGAACATTTTATGAGAGCAGTTGCAAGGCAACCAACTCCATTGAAATCAAGGACAAGGCAAGAGACCAGGAAATTGCTAGTCTGAGAACTGTTCTACAGTGTCTTTCAGATAACAATATTGAATCTGCGGATTTACTCAACGAGATTCACAGTAGAATTCTTGAGCTAAATAGAGAGAAAGGCGAGTAG
Protein sequence

>Msa0232690

MVVRASKRKKLLNLYDSKSDDDYDVPLRAKSLSTLNLGASSSSSSSKSIENRPTRLPAKRPFDDSISSQTSVKKSKLSSFSIKLMKKSILGLRSVEEEKLQSLERDIEECSKELLNKKKQASDVRRTNQYYEEMQNKIEKGVKDLAANEAHVRFIEGLIKNKTLELKKNKRQLLTAMDSNSKGRGRLKEKELETLSQKIAECTQEIKTRKEELDASKISVNHKIKELMSERSNILNVMSERRTGQLVQMKDLESTEKQFEGGATEFDSKMKQCDRRLEGVESNEKLYKGQTKVSESKQEEFESQVKELESKKKQFESQEKVLGLKEKLFQRQVDDLESCKEHFESQLKGLKSKEKIFERRMKELKSKEERFQRRVKEFELRECDFEGQVKDFESKLKQYEGQVKELQSKREEFEGRVEEFKSQEKDFESRVKGFESKKKDFESRVTKFETVEKDFEIRVRKFKSVEKDFESRVRKFESVEKDFESRVRKFESAEKDFESRVKKFESKEEELELRDGQYETLIKSFEEEIESANYAISSFTDDQPSPAIDGRSLQFLPIEEIDELESHGNDSLANLLASSSDPSKDVLDIIQNPIIPQCKGENVVIIDDHHIDLLEQLMRISPHVKPHVREEAMKLALKLKAYIGENTANPVPVLGFLLLLSIYGLVSSFDEDEVLKLFGFAAQHKISVELFGTMGLAHKVSDLVQNLIMKQQYIEAVRFICYNTATKNQSVDLLREHVQNARTFYESSCKATNSIEIKDKARDQEIASLRTVLQCLSDNNIESADLLNEIHSRILELNREKGE*