AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0829800

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0829800 MtrunA17_Chr2g0280141 89.359 827 55 4 1 803 1 818 0.0 1400
Msa0829800 MtrunA17_Chr2g0325811 45.204 834 363 16 19 801 40 830 1.32e-180 539
Msa0829800 MtrunA17_Chr2g0325711 42.962 817 393 15 10 783 31 817 2.40e-178 533
Msa0829800 MtrunA17_Chr2g0325661 42.938 885 354 19 16 802 45 876 2.67e-165 501
Msa0829800 MtrunA17_Chr2g0325861 42.131 826 324 14 6 727 71 846 2.78e-161 490
Msa0829800 MtrunA17_Chr2g0325361 48.505 602 262 7 248 802 477 1077 7.90e-137 432
Msa0829800 MtrunA17_Chr2g0325361 35.636 550 295 8 25 529 13 548 4.82e-59 218
Msa0829800 MtrunA17_Chr6g0471251 39.083 829 348 19 19 802 51 767 2.69e-134 417
Msa0829800 MtrunA17_Chr2g0325321 41.365 747 351 12 42 704 18 761 1.02e-126 397
Msa0829800 MtrunA17_Chr2g0325611 51.599 469 187 9 359 802 137 590 1.66e-124 386
Msa0829800 MtrunA17_Chr2g0325611 38.023 263 106 7 188 427 93 321 1.56e-26 115
Msa0829800 MtrunA17_Chr2g0325231 36.637 898 357 22 19 721 16 896 2.49e-124 395
Msa0829800 MtrunA17_Chr2g0325351 45.804 572 267 11 247 802 144 688 2.57e-116 367
Msa0829800 MtrunA17_Chr2g0325351 36.937 333 140 6 93 414 142 415 9.18e-33 135
Msa0829800 MtrunA17_Chr2g0325351 34.899 298 156 7 179 456 136 415 1.14e-23 107
Msa0829800 MtrunA17_Chr2g0325621 42.652 558 275 8 272 802 121 660 5.56e-109 347
Msa0829800 MtrunA17_Chr2g0325621 34.247 438 201 8 12 415 3 387 1.05e-37 150
Msa0829800 MtrunA17_Chr5g0434761 61.132 265 99 4 541 802 2 265 5.72e-92 289
Msa0829800 MtrunA17_Chr2g0325701 38.403 526 237 11 183 701 144 589 4.06e-78 263
Msa0829800 MtrunA17_Chr2g0325631 59.245 265 104 4 541 802 2 265 1.08e-76 249
Msa0829800 MtrunA17_Chr3g0140591 74.405 168 42 1 537 704 6 172 2.13e-75 242
Msa0829800 MtrunA17_Chr2g0325281 36.393 610 291 11 15 539 3 600 1.41e-73 251
Msa0829800 MtrunA17_Chr2g0325221 35.852 569 292 9 19 530 45 597 5.73e-72 249
Msa0829800 MtrunA17_Chr2g0325221 61.386 101 38 1 702 801 599 699 3.71e-28 121
Msa0829800 MtrunA17_Chr5g0416271 38.446 489 245 10 216 701 47 482 6.53e-71 241
Msa0829800 MtrunA17_Chr5g0416271 33.832 334 178 8 43 364 14 316 2.45e-26 113
Msa0829800 MtrunA17_Chr2g0325291 64.878 205 69 3 556 758 3 206 4.51e-70 230
Msa0829800 MtrunA17_Chr2g0333341 45.808 334 136 7 389 721 332 621 2.50e-64 227
Msa0829800 MtrunA17_Chr2g0333341 35.096 416 189 12 25 396 72 450 1.14e-37 150
Msa0829800 MtrunA17_Chr4g0044221 57.672 189 79 1 82 269 1 189 2.87e-55 193
Msa0829800 MtrunA17_Chr4g0044221 63.226 155 28 2 486 639 168 294 2.16e-54 191
Msa0829800 MtrunA17_Chr2g0325241 41.846 325 161 6 312 627 120 425 3.64e-52 187
Msa0829800 MtrunA17_Chr2g0325241 37.073 205 124 2 248 449 126 328 2.76e-20 94.4
Msa0829800 MtrunA17_Chr2g0332961 42.857 322 136 11 368 674 65 353 5.88e-48 174
Msa0829800 MtrunA17_Chr2g0325721 35.094 530 241 12 22 505 29 501 4.94e-47 175
Msa0829800 MtrunA17_Chr2g0325721 31.635 373 208 9 173 512 108 466 6.82e-19 90.9
Msa0829800 MtrunA17_Chr2g0332951 47.807 228 94 8 483 704 2 210 2.47e-44 159
Msa0829800 MtrunA17_Chr2g0325761 31.029 651 329 13 21 606 53 648 3.63e-44 170
Msa0829800 MtrunA17_Chr2g0325761 62.245 98 36 1 701 797 591 688 2.58e-28 121
Msa0829800 MtrunA17_Chr2g0325771 58.046 174 61 3 534 704 11 175 6.89e-42 150
Msa0829800 MtrunA17_Chr8g0361651 34.673 398 231 11 417 800 23 405 9.95e-41 156
Msa0829800 MtrunA17_Chr2g0325331 58.170 153 62 2 542 693 3 154 1.83e-38 140
Msa0829800 MtrunA17_Chr4g0024061 30.395 658 345 26 191 800 59 651 2.04e-38 153
Msa0829800 MtrunA17_Chr2g0325691 34.120 466 218 13 122 527 283 719 8.98e-38 151
Msa0829800 MtrunA17_Chr2g0325691 37.048 332 192 4 185 512 166 484 1.55e-31 132
Msa0829800 MtrunA17_Chr2g0325691 37.500 272 148 4 296 552 223 487 1.45e-25 113
Msa0829800 MtrunA17_Chr2g0325651 34.359 390 158 8 56 409 59 386 1.23e-37 145
Msa0829800 MtrunA17_Chr2g0325651 45.517 145 69 2 389 526 236 377 2.96e-22 99.8
Msa0829800 MtrunA17_Chr2g0325651 45.033 151 72 2 375 514 236 386 1.56e-21 97.8
Msa0829800 MtrunA17_Chr7g0235901 36.034 358 170 5 61 415 10 311 7.72e-36 138
Msa0829800 MtrunA17_Chr7g0235901 36.406 217 100 2 296 512 132 310 1.67e-20 93.2
Msa0829800 MtrunA17_Chr7g0235901 33.673 196 125 2 339 530 92 286 1.05e-12 70.1
Msa0829800 MtrunA17_Chr2g0325311 63.107 103 36 2 702 802 17 119 1.47e-31 119
Msa0829800 MtrunA17_Chr2g0325641 37.091 275 166 1 249 523 1 268 3.62e-30 122
Msa0829800 MtrunA17_Chr2g0325641 36.336 333 189 5 212 538 3 318 1.12e-29 120
Msa0829800 MtrunA17_Chr2g0325641 37.795 254 133 5 296 542 6 241 5.32e-23 100
Msa0829800 MtrunA17_Chr2g0325641 35.385 260 130 5 190 418 58 310 1.34e-22 99.8
Msa0829800 MtrunA17_Chr3g0140581 55.147 136 59 1 92 227 21 154 1.48e-29 115
Msa0829800 MtrunA17_Chr2g0325271 54.808 104 45 2 701 802 40 143 5.25e-27 107
Msa0829800 MtrunA17_Chr2g0325201 35.865 237 137 2 118 354 2 223 3.57e-26 107
Msa0829800 MtrunA17_Chr2g0325261 32.588 313 125 6 56 333 45 306 1.38e-23 102
Msa0829800 MtrunA17_Chr2g0325261 51.220 82 40 0 375 456 222 303 2.28e-12 68.9
Msa0829800 MtrunA17_Chr2g0325261 31.556 225 142 4 292 512 87 303 6.60e-11 64.3
Msa0829800 MtrunA17_Chr2g0325341 32.588 313 125 6 56 333 45 306 1.38e-23 102
Msa0829800 MtrunA17_Chr2g0325341 51.220 82 40 0 375 456 222 303 2.28e-12 68.9
Msa0829800 MtrunA17_Chr2g0325341 31.556 225 142 4 292 512 87 303 6.60e-11 64.3
Msa0829800 MtrunA17_Chr5g0434751 37.500 280 147 2 249 514 1 266 1.07e-22 99.4
Msa0829800 MtrunA17_Chr5g0434751 35.565 239 136 3 188 419 35 262 7.00e-16 79.3
Msa0829800 MtrunA17_Chr5g0434751 38.073 218 121 2 248 458 56 266 2.38e-15 77.8
Msa0829800 MtrunA17_Chr2g0325781 31.921 354 125 9 16 341 40 305 2.80e-22 98.6
Msa0829800 MtrunA17_Chr2g0325781 42.241 116 43 1 296 411 214 305 4.17e-12 68.2
Msa0829800 MtrunA17_Chr5g0434741 31.373 306 123 7 56 326 46 299 3.06e-22 98.2
Msa0829800 MtrunA17_Chr7g0235921 59.794 97 38 1 618 714 1 96 2.10e-20 87.0
Msa0829800 MtrunA17_Chr4g0053711 32.143 252 145 8 576 803 194 443 4.43e-20 94.7
Msa0829800 MtrunA17_Chr2g0325751 42.038 157 42 3 249 405 7 114 7.51e-17 78.6
Msa0829800 MtrunA17_Chr2g0325751 36.242 149 94 1 403 550 7 155 5.64e-16 76.3
Msa0829800 MtrunA17_Chr2g0325751 46.087 115 55 2 305 419 7 114 3.67e-14 70.9
Msa0829800 MtrunA17_Chr2g0325791 46.825 126 57 1 310 435 41 156 1.75e-16 81.6
Msa0829800 MtrunA17_Chr2g0325791 39.683 126 66 1 352 477 41 156 3.05e-13 71.6
Msa0829800 MtrunA17_Chr2g0325741 29.395 347 117 12 16 318 42 304 4.03e-16 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0829800 AT5G27220.1 28.079 203 134 7 581 778 872 1067 4.13e-15 80.5
Msa0829800 AT5G27220.1 25.542 646 406 20 76 697 208 802 9.87e-14 75.9
Msa0829800 AT5G27220.3 28.079 203 134 7 581 778 872 1067 4.13e-15 80.5
Msa0829800 AT5G27220.3 25.542 646 406 20 76 697 208 802 9.87e-14 75.9
Msa0829800 AT5G27220.4 25.542 646 406 20 76 697 208 802 6.22e-14 76.6
Msa0829800 AT5G27220.4 27.815 151 101 4 630 778 868 1012 5.77e-12 70.1
Msa0829800 AT5G27220.2 25.542 646 406 20 76 697 208 802 6.22e-14 76.6
Msa0829800 AT5G27220.2 27.815 151 101 4 630 778 868 1012 5.77e-12 70.1
Msa0829800 AT1G31814.1 26.786 224 150 6 569 783 117 335 1.85e-13 73.9
Msa0829800 AT5G48385.1 27.333 150 108 1 655 803 308 457 2.58e-13 73.9
Msa0829800 AT5G27230.1 27.056 377 212 16 423 755 38 395 3.92e-13 73.9

Find 162 sgRNAs with CRISPR-Local

Find 249 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCATTGTCAACTTTGAATT+TGG 0.089700 5_4:+2810921 Msa0829800:CDS
TTGCTCCTTCTGTCAATTTA+TGG 0.102499 5_4:+2812885 Msa0829800:CDS
GATAGATAGTATTATAATTT+TGG 0.129006 5_4:+2814133 Msa0829800:three_prime_UTR
CCAAAGTGAAACACTGTATT+TGG 0.182293 5_4:-2810190 None:intergenic
GGTTTGGCACATAAAGTTTC+TGG 0.195386 5_4:+2812999 Msa0829800:CDS
GCTCTTCTTCATGAGCTTAA+TGG 0.209076 5_4:-2811073 None:intergenic
CAGGATTCTGCGTTAAGATT+TGG 0.231497 5_4:+2814087 Msa0829800:three_prime_UTR
TGTAGAGCTGTTTGGGACTA+TGG 0.236978 5_4:+2812977 Msa0829800:CDS
TTTCTTATTTAGAAGCTCTT+TGG 0.268782 5_4:-2811163 None:intergenic
TCAAATGAGAAGCTCTATAA+AGG 0.280877 5_4:+2811675 Msa0829800:CDS
GTAGTTCAATAATGGTTGTT+AGG 0.295009 5_4:+2810821 Msa0829800:exon
AAGATTTGGGATTCAGTTCT+TGG 0.304088 5_4:+2814101 Msa0829800:three_prime_UTR
AGTTCATCAATTTCCTCAAT+TGG 0.304511 5_4:-2812585 None:intergenic
AGGATTCTGCGTTAAGATTT+GGG 0.318411 5_4:+2814088 Msa0829800:three_prime_UTR
TGCAAATCCAGTACCAGTTC+TGG 0.327369 5_4:+2812857 Msa0829800:CDS
CAAATACAGTGTTTCACTTT+GGG 0.336751 5_4:+2810191 Msa0829800:five_prime_UTR
ATGCAGAACAAAATCGAAAA+AGG 0.349065 5_4:+2811231 Msa0829800:CDS
CCTTCTGTCAATTTATGGAT+TGG 0.349385 5_4:+2812890 Msa0829800:CDS
TCATAATCAAAACATCAAAA+TGG 0.358355 5_4:-2814305 None:intergenic
CTTCCATCAATGGCAGGACT+TGG 0.360593 5_4:-2812546 None:intergenic
TCGGTCACACTGCTTCATTT+TGG 0.361776 5_4:-2811634 None:intergenic
TCATTGTCAACTTTGAATTT+GGG 0.364609 5_4:+2810922 Msa0829800:CDS
TCATCAAAAGGCCTCTTTGC+TGG 0.367743 5_4:-2811000 None:intergenic
AGATATCTGTAGAGCTGTTT+GGG 0.367948 5_4:+2812970 Msa0829800:CDS
AAGATATCTGTAGAGCTGTT+TGG 0.368664 5_4:+2812969 Msa0829800:CDS
GCTGTTTGGGACTATGGGTT+TGG 0.369030 5_4:+2812983 Msa0829800:CDS
CCCCATCATTCCACAGTGTA+AGG 0.370588 5_4:+2812692 Msa0829800:CDS
CAACCAAGTCCTGCCATTGA+TGG 0.374788 5_4:+2812543 Msa0829800:CDS
AGAAGAGCATACTCGGATTA+AGG 0.374809 5_4:+2811088 Msa0829800:CDS
TTTGAAGAGGAAATTGAATC+AGG 0.376038 5_4:+2812404 Msa0829800:CDS
TCTGCATTTCCTCGTAATAT+TGG 0.377971 5_4:-2811215 None:intergenic
TTCTCAGACTAGCAATTTCC+TGG 0.380055 5_4:-2813915 None:intergenic
TGGGATTCAGTTCTTGGATG+TGG 0.380091 5_4:+2814107 Msa0829800:three_prime_UTR
GAGAGAAAGGCGAGTAGCTT+TGG 0.383992 5_4:+2814022 Msa0829800:exon
CCCTTACACTGTGGAATGAT+GGG 0.386689 5_4:-2812693 None:intergenic
GTTCATCAATTTCCTCAATT+GGG 0.386900 5_4:-2812584 None:intergenic
TGATTTGTGTAGTTCAATAA+TGG 0.390713 5_4:+2810813 Msa0829800:exon
TCCCTTACACTGTGGAATGA+TGG 0.397453 5_4:-2812694 None:intergenic
AGGACTTTGAAAGCCGAGTT+AGG 0.398394 5_4:+2812231 Msa0829800:CDS
AGGACTTTGAAAGCCGAGTT+AGG 0.398394 5_4:+2812273 Msa0829800:CDS
GACTTTGAAAGCCGAGTAAA+GGG 0.402680 5_4:+2812107 Msa0829800:CDS
CAGTGTGACCGACGACTAGA+AGG 0.410719 5_4:+2811645 Msa0829800:CDS
CCAAATACAGTGTTTCACTT+TGG 0.420774 5_4:+2810190 Msa0829800:five_prime_UTR
TAGTTCAATAATGGTTGTTA+GGG 0.427649 5_4:+2810822 Msa0829800:exon
GTAGAGCTGTTTGGGACTAT+GGG 0.430125 5_4:+2812978 Msa0829800:CDS
AAGGCGAGTAGCTTTGGTAT+TGG 0.430765 5_4:+2814028 Msa0829800:exon
TCAATCAGTTGATCTATTGC+GGG 0.431042 5_4:+2813719 Msa0829800:CDS
CTCTTTGCTGGCAGTCTAGT+AGG 0.431702 5_4:-2810988 None:intergenic
AACCTTGATTTCAATGGAGT+TGG 0.432488 5_4:-2813790 None:intergenic
TTGATTTGAGTCCCTTCAGT+TGG 0.434408 5_4:-2811866 None:intergenic
GATATTTGAAAGACGGATGA+AGG 0.437782 5_4:+2811896 Msa0829800:CDS
CTGTTTCAAACTTCGTCACT+CGG 0.440261 5_4:-2812160 None:intergenic
AACCAACTCCATTGAAATCA+AGG 0.448934 5_4:+2813788 Msa0829800:CDS
TCAAAGACAAGTAGATGATC+TGG 0.452367 5_4:+2811818 Msa0829800:CDS
GAAATATTGAGATAACATTA+CGG 0.455477 5_4:+2814387 Msa0829800:three_prime_UTR
TTTGCAGTTTCTCCCAATTG+AGG 0.456677 5_4:+2812572 Msa0829800:CDS
CTGATTTGAACTTCCTCACT+CGG 0.460825 5_4:-2812202 None:intergenic
GCCTTTACTATTGCTATCCA+TGG 0.462841 5_4:-2811358 None:intergenic
GTAGCTTTGGTATTGGAAGT+AGG 0.465926 5_4:+2814035 Msa0829800:exon
TAATCAAACCCTCGATGAAA+CGG 0.467409 5_4:-2811287 None:intergenic
TACTTACCTTTGTGTGGCTG+AGG 0.468990 5_4:-2810298 None:intergenic
ACTGTGGAATGATGGGGTTC+TGG 0.470582 5_4:-2812686 None:intergenic
GTAATGTCAGAGAGGAGAAC+TGG 0.471534 5_4:+2811540 Msa0829800:CDS
GTTGTCTCATTTACAAACTT+CGG 0.474404 5_4:-2814065 None:intergenic
AGGATGACAGTAATGAGAAC+TGG 0.474459 5_4:+2814232 Msa0829800:three_prime_UTR
GTAGTAAATCTATTGAGAAT+AGG 0.474756 5_4:+2810965 Msa0829800:CDS
GCAAATCCAGTACCAGTTCT+GGG 0.480024 5_4:+2812858 Msa0829800:CDS
GCAGGAGGAATTCGAAAGTC+AGG 0.484190 5_4:+2811722 Msa0829800:CDS
GCAATTTGAAAGCCAGGAAA+AGG 0.485355 5_4:+2811770 Msa0829800:CDS
CTCATGAAGAAGAGCATACT+CGG 0.485463 5_4:+2811081 Msa0829800:CDS
CAAATGAGAAGCTCTATAAA+GGG 0.486869 5_4:+2811676 Msa0829800:CDS
CTGCAAACTTCTTCCATCAA+TGG 0.487566 5_4:-2812556 None:intergenic
CTGATTCAAACTTCCTAACT+CGG 0.487972 5_4:-2812286 None:intergenic
GCTTGTTCAAATGAAGGACT+TGG 0.488868 5_4:+2811566 Msa0829800:CDS
AAGTAGGAATGAAATGAGAA+AGG 0.489576 5_4:-2810261 None:intergenic
CTGATTCAAACTTCCTAACT+CGG 0.490086 5_4:-2812244 None:intergenic
AAAGAAGCAATTTGAAAGCC+AGG 0.492303 5_4:+2811764 Msa0829800:CDS
GAAGTTTGAATCAGTAGAGA+AGG 0.504108 5_4:+2812253 Msa0829800:CDS
TTGATTCAAACCCCTTTACT+CGG 0.505676 5_4:-2812118 None:intergenic
AAGTTTGTAAATGAGACAAC+AGG 0.507654 5_4:+2814068 Msa0829800:three_prime_UTR
GGGATTCAGTTCTTGGATGT+GGG 0.508374 5_4:+2814108 Msa0829800:three_prime_UTR
TAGCTTTGGTATTGGAAGTA+GGG 0.508548 5_4:+2814036 Msa0829800:exon
GAAGTTTGAATCAGCAGAGA+AGG 0.509904 5_4:+2812295 Msa0829800:CDS
GGACTTTGAAAGCCGAGTAA+AGG 0.510913 5_4:+2812106 Msa0829800:CDS
TAAAGGCCGTGGAAGATTGA+AGG 0.511019 5_4:+2811374 Msa0829800:CDS
ATCAATCAGTTGATCTATTG+CGG 0.512272 5_4:+2813718 Msa0829800:CDS
GGAGAGTGAACTAAGAAAGT+AGG 0.514540 5_4:-2810277 None:intergenic
GAAGTTCAAATCAGTAGAGA+AGG 0.514891 5_4:+2812211 Msa0829800:CDS
GTTAATAAAATCATTTGAAG+AGG 0.515437 5_4:+2812391 Msa0829800:CDS
TTGATTCAAACTTCTTCACT+CGG 0.515510 5_4:-2812328 None:intergenic
TCAAAATTGAAGCAATATGA+AGG 0.517246 5_4:+2812011 Msa0829800:CDS
GCGCATGTCCGTTTCATCGA+GGG 0.520061 5_4:+2811279 Msa0829800:CDS
TCAATATTTCAAAGCAATTG+AGG 0.523088 5_4:-2814374 None:intergenic
GAGGAAATGCTGTCATCAAA+AGG 0.524403 5_4:-2811012 None:intergenic
CTTGAGCTAAATAGAGAGAA+AGG 0.526158 5_4:+2814009 Msa0829800:CDS
ATCCCTTGAGAGAGATATTG+AGG 0.530827 5_4:+2811134 Msa0829800:CDS
CAGCATTTCCTCGCAAACTT+CGG 0.531960 5_4:+2811023 Msa0829800:CDS
TAAAAGAACTAATGTCAGAA+AGG 0.536181 5_4:+2811502 Msa0829800:CDS
AAAGGTGTCGGAGTCAAAGC+AGG 0.537649 5_4:+2811704 Msa0829800:CDS
GAAGTTTGAATCAAAAGAAG+AGG 0.538867 5_4:+2812337 Msa0829800:CDS
TGGTCAGCTTGTTCAAATGA+AGG 0.542980 5_4:+2811560 Msa0829800:CDS
ATTCAACCCCTTCTAGTCGT+CGG 0.546192 5_4:-2811653 None:intergenic
TTGATTCGAAGTCCTTCACC+TGG 0.546907 5_4:-2811992 None:intergenic
CTTAAACTGAAAGCTTACAT+TGG 0.548401 5_4:+2812825 Msa0829800:CDS
AGAGTTTAAATCACAAGAGA+AGG 0.548434 5_4:+2812085 Msa0829800:CDS
TCAAAGAGAGAGGAATTTGA+AGG 0.551665 5_4:+2812053 Msa0829800:CDS
GACAATGACTTTGCTCTCAA+AGG 0.551969 5_4:-2810907 None:intergenic
ACTCCTCAATATCTCTCTCA+AGG 0.552126 5_4:-2811137 None:intergenic
TCAGGACAAGGCAAGAGACC+AGG 0.554657 5_4:+2813897 Msa0829800:intron
AAACTTCTTCCATCAATGGC+AGG 0.555447 5_4:-2812552 None:intergenic
ACAATTTACTTACCTTTGTG+TGG 0.555870 5_4:-2810304 None:intergenic
AGATAACAATATTGAATCTG+CGG 0.559408 5_4:+2813957 Msa0829800:CDS
AAGAGAAGATATTTGAAAGA+CGG 0.560688 5_4:+2811889 Msa0829800:CDS
TCAACGGAAAAGCAATTTGA+AGG 0.560873 5_4:+2811591 Msa0829800:CDS
ATTGCTTCTTCTCGCACATG+AGG 0.561232 5_4:-2812792 None:intergenic
GCAATATGAAGGACAAGTGA+AGG 0.566613 5_4:+2812022 Msa0829800:CDS
GAAAGCCAGGAAAAGGTGCT+CGG 0.567009 5_4:+2811777 Msa0829800:CDS
AAGTGAAACACTGTATTTGG+TGG 0.570485 5_4:-2810187 None:intergenic
TCAGGAAATTAAGACGAGAA+AGG 0.571467 5_4:+2811440 Msa0829800:CDS
GAAGTTTGAAACAGTAGAGA+AGG 0.571865 5_4:+2812169 Msa0829800:CDS
GGAATTCGAAAGTCAGGTGA+AGG 0.576331 5_4:+2811728 Msa0829800:CDS
GTGATTTAAACTCTTCCACT+CGG 0.576446 5_4:-2812076 None:intergenic
GATAGCAATAGTAAAGGCCG+TGG 0.579238 5_4:+2811363 Msa0829800:CDS
CGGATTAAGGAGCGTTGAGG+AGG 0.584542 5_4:+2811101 Msa0829800:CDS
TTTCTGCCCAGGATTTGAAG+TGG 0.585302 5_4:+2810790 Msa0829800:intron
GAAGGAGTTGAAGTCAAAAG+AGG 0.587532 5_4:+2811914 Msa0829800:CDS
CGCACATGAGGTTTAACATG+TGG 0.590019 5_4:-2812780 None:intergenic
CCAATCCATAAATTGACAGA+AGG 0.591953 5_4:-2812890 None:intergenic
GGAGTTACAATCAAAGAGAG+AGG 0.595701 5_4:+2812043 Msa0829800:CDS
GCCATGGATAGCAATAGTAA+AGG 0.596670 5_4:+2811357 Msa0829800:CDS
GAGACAACTTCTTACAGCCA+TGG 0.598031 5_4:+2811341 Msa0829800:CDS
CCCATCATTCCACAGTGTAA+GGG 0.604052 5_4:+2812693 Msa0829800:CDS
AATGAAGGACTTGGAGTCAA+CGG 0.606158 5_4:+2811575 Msa0829800:CDS
CCTTACACTGTGGAATGATG+GGG 0.610080 5_4:-2812692 None:intergenic
CTGATCGAGACACAAGGGGA+AGG 0.618054 5_4:+2814212 Msa0829800:three_prime_UTR
TAAAGGGCAGACAAAGGTGT+CGG 0.621702 5_4:+2811692 Msa0829800:CDS
CTCCTCAATATCTCTCTCAA+GGG 0.622421 5_4:-2811136 None:intergenic
TAGATTCATTTGTTATAACA+CGG 0.623887 5_4:+2813686 Msa0829800:CDS
AGTGTGACCGACGACTAGAA+GGG 0.627275 5_4:+2811646 Msa0829800:CDS
TTTATCTGATCGAGACACAA+GGG 0.627670 5_4:+2814207 Msa0829800:three_prime_UTR
ATTTATCTGATCGAGACACA+AGG 0.627865 5_4:+2814206 Msa0829800:three_prime_UTR
GAGGAACTTGAGCTAAGAGA+CGG 0.629178 5_4:+2812356 Msa0829800:CDS
AGAGGAATTTGAAGGCCGAG+TGG 0.631001 5_4:+2812061 Msa0829800:CDS
GCTCTATAAAGGGCAGACAA+AGG 0.633152 5_4:+2811686 Msa0829800:CDS
AGATGATCTGGAATCATGCA+AGG 0.638630 5_4:+2811830 Msa0829800:CDS
CACTCTCCTCAGCCACACAA+AGG 0.642678 5_4:+2810292 Msa0829800:five_prime_UTR
ACGGAAAAGCAATTTGAAGG+TGG 0.647692 5_4:+2811594 Msa0829800:CDS
AGCGCATGTCCGTTTCATCG+AGG 0.649306 5_4:+2811278 Msa0829800:CDS
CAAATCCAGTACCAGTTCTG+GGG 0.656039 5_4:+2812859 Msa0829800:CDS
GTGTGACCGACGACTAGAAG+GGG 0.661302 5_4:+2811647 Msa0829800:CDS
CATCAAAATGGTACAATACG+AGG 0.667651 5_4:-2814293 None:intergenic
CTACTAGACTGCCAGCAAAG+AGG 0.670048 5_4:+2810989 Msa0829800:CDS
CTTTGGTATTGGAAGTAGGG+CGG 0.670313 5_4:+2814039 Msa0829800:three_prime_UTR
TACTAAATGTAATGTCAGAG+AGG 0.673630 5_4:+2811532 Msa0829800:CDS
AGGACTTTGAAATCCGAGTG+AGG 0.681019 5_4:+2812189 Msa0829800:CDS
ACTTTGAAAGCCGAGTAAAG+GGG 0.687533 5_4:+2812108 Msa0829800:CDS
ACTCGGATTAAGGAGCGTTG+AGG 0.688313 5_4:+2811098 Msa0829800:CDS
GGTGTCGGAGTCAAAGCAGG+AGG 0.695615 5_4:+2811707 Msa0829800:CDS
TTATCTGATCGAGACACAAG+GGG 0.696466 5_4:+2814208 Msa0829800:three_prime_UTR
ATTGATGAACTTGAGTCACA+CGG 0.701580 5_4:+2812597 Msa0829800:CDS
ACGAACTAACCAATATTACG+AGG 0.703439 5_4:+2811206 Msa0829800:CDS
GTTGTTCGAGCAGATCAATG+TGG 0.704073 5_4:-2812743 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTAAATGATAAAAAAAAAAA+AGG - chr5_4:2810356-2810375 None:intergenic 10.0%
!! AACATTAAAAATTTATTGAA+TGG - chr5_4:2810399-2810418 None:intergenic 10.0%
!! ATCAAATGTATAAATATATT+TGG + chr5_4:2813139-2813158 Msa0829800:intron 10.0%
!! TTATAAAAAAGTTAGCATAT+TGG + chr5_4:2813285-2813304 Msa0829800:intron 15.0%
!! ATAACATCTCAAAATAAATT+GGG - chr5_4:2813571-2813590 None:intergenic 15.0%
!! AATAACATCTCAAAATAAAT+TGG - chr5_4:2813572-2813591 None:intergenic 15.0%
!! ATTATTTATAAATAACATGC+AGG - chr5_4:2813865-2813884 None:intergenic 15.0%
!!! AATAATTTCAATTTGAAATC+AGG + chr5_4:2813879-2813898 Msa0829800:intron 15.0%
!!! GATAGATAGTATTATAATTT+TGG + chr5_4:2814133-2814152 Msa0829800:three_prime_UTR 15.0%
!!! ATAGTATTATAATTTTGGAA+TGG + chr5_4:2814138-2814157 Msa0829800:three_prime_UTR 15.0%
!!! TTTTATCATTTACATTTCCA+TGG + chr5_4:2810363-2810382 Msa0829800:intron 20.0%
!! ATTTGCAGCTTATATATAAA+CGG - chr5_4:2810695-2810714 None:intergenic 20.0%
!!! ATTAAGTTGTTTATCCAAAA+AGG + chr5_4:2810718-2810737 Msa0829800:intron 20.0%
!!! TTAAGTTGTTTATCCAAAAA+GGG + chr5_4:2810719-2810738 Msa0829800:intron 20.0%
!! GTTAATAAAATCATTTGAAG+AGG + chr5_4:2812391-2812410 Msa0829800:CDS 20.0%
!!! TATCTAAAACATCTTTTGAT+GGG - chr5_4:2812662-2812681 None:intergenic 20.0%
!!! ATATCTAAAACATCTTTTGA+TGG - chr5_4:2812663-2812682 None:intergenic 20.0%
!!! ATGAAGTTTTAAAGCTTTTT+GGG + chr5_4:2812931-2812950 Msa0829800:CDS 20.0%
!!! ACAAATCAGAATTAACTTTT+GGG - chr5_4:2813266-2813285 None:intergenic 20.0%
!!! AACAAATCAGAATTAACTTT+TGG - chr5_4:2813267-2813286 None:intergenic 20.0%
!! ATATATCTTTCTAATTCAAG+TGG + chr5_4:2813340-2813359 Msa0829800:intron 20.0%
!! TAACATCTCAAAATAAATTG+GGG - chr5_4:2813570-2813589 None:intergenic 20.0%
!!! TAGATTCATTTGTTATAACA+CGG + chr5_4:2813686-2813705 Msa0829800:CDS 20.0%
!! AAAAAAAACCTTGATTTCAA+TGG - chr5_4:2813799-2813818 None:intergenic 20.0%
!! TCATAATCAAAACATCAAAA+TGG - chr5_4:2814308-2814327 None:intergenic 20.0%
!! GAAATATTGAGATAACATTA+CGG + chr5_4:2814387-2814406 Msa0829800:three_prime_UTR 20.0%
!! TGATTTGTGTAGTTCAATAA+TGG + chr5_4:2810813-2810832 Msa0829800:exon 25.0%
! TAGTTCAATAATGGTTGTTA+GGG + chr5_4:2810822-2810841 Msa0829800:exon 25.0%
! TCATTGTCAACTTTGAATTT+GGG + chr5_4:2810922-2810941 Msa0829800:CDS 25.0%
! GTAGTAAATCTATTGAGAAT+AGG + chr5_4:2810965-2810984 Msa0829800:CDS 25.0%
!! TTTCTTATTTAGAAGCTCTT+TGG - chr5_4:2811166-2811185 None:intergenic 25.0%
! TAAAAGAACTAATGTCAGAA+AGG + chr5_4:2811502-2811521 Msa0829800:CDS 25.0%
! AAGAGAAGATATTTGAAAGA+CGG + chr5_4:2811889-2811908 Msa0829800:CDS 25.0%
! TCAAAATTGAAGCAATATGA+AGG + chr5_4:2812011-2812030 Msa0829800:CDS 25.0%
!!! GATGAAGTTTTAAAGCTTTT+TGG + chr5_4:2812930-2812949 Msa0829800:CDS 25.0%
! ACTAAGAAATTGAACTGATA+TGG + chr5_4:2813193-2813212 Msa0829800:intron 25.0%
! AAATTGAACTGATATGGAAT+TGG + chr5_4:2813199-2813218 Msa0829800:intron 25.0%
!! AAATCAGAATTAACTTTTGG+GGG - chr5_4:2813264-2813283 None:intergenic 25.0%
!! CAAATCAGAATTAACTTTTG+GGG - chr5_4:2813265-2813284 None:intergenic 25.0%
! TGGTTAAAGAGTTTCAATTA+AGG + chr5_4:2813360-2813379 Msa0829800:intron 25.0%
!!! CATATGTGCTGTTTTTTTAA+TGG + chr5_4:2813523-2813542 Msa0829800:intron 25.0%
!!! TAGATCAACTGATTGATTTT+TGG - chr5_4:2813715-2813734 None:intergenic 25.0%
! AGATAACAATATTGAATCTG+CGG + chr5_4:2813957-2813976 Msa0829800:CDS 25.0%
! AACAAACTGATGTAAAAATC+TGG + chr5_4:2814263-2814282 Msa0829800:three_prime_UTR 25.0%
! TCAATATTTCAAAGCAATTG+AGG - chr5_4:2814377-2814396 None:intergenic 25.0%
CAAATACAGTGTTTCACTTT+GGG + chr5_4:2810191-2810210 Msa0829800:five_prime_UTR 30.0%
AAGTAGGAATGAAATGAGAA+AGG - chr5_4:2810264-2810283 None:intergenic 30.0%
ACAATTTACTTACCTTTGTG+TGG - chr5_4:2810307-2810326 None:intergenic 30.0%
TATCAATCACAAATCAGCTA+GGG - chr5_4:2810453-2810472 None:intergenic 30.0%
! ATTAGTCTAAGAAAACATGG+GGG - chr5_4:2810482-2810501 None:intergenic 30.0%
! CATTAGTCTAAGAAAACATG+GGG - chr5_4:2810483-2810502 None:intergenic 30.0%
! GCATTAGTCTAAGAAAACAT+GGG - chr5_4:2810484-2810503 None:intergenic 30.0%
! TGCATTAGTCTAAGAAAACA+TGG - chr5_4:2810485-2810504 None:intergenic 30.0%
CTTATAACATGAGATCTGTT+TGG + chr5_4:2810519-2810538 Msa0829800:intron 30.0%
!! AATAGTATCAAAGGTGCTTA+TGG - chr5_4:2810572-2810591 None:intergenic 30.0%
! TCAACTATTAAGACCCTTTT+TGG - chr5_4:2810735-2810754 None:intergenic 30.0%
GTAGTTCAATAATGGTTGTT+AGG + chr5_4:2810821-2810840 Msa0829800:exon 30.0%
GTCATTGTCAACTTTGAATT+TGG + chr5_4:2810921-2810940 Msa0829800:CDS 30.0%
ATGCAGAACAAAATCGAAAA+AGG + chr5_4:2811231-2811250 Msa0829800:CDS 30.0%
AAAAATTGCTGAATGTACTC+AGG + chr5_4:2811422-2811441 Msa0829800:CDS 30.0%
TACTAAATGTAATGTCAGAG+AGG + chr5_4:2811532-2811551 Msa0829800:CDS 30.0%
TCAAATGAGAAGCTCTATAA+AGG + chr5_4:2811675-2811694 Msa0829800:CDS 30.0%
CAAATGAGAAGCTCTATAAA+GGG + chr5_4:2811676-2811695 Msa0829800:CDS 30.0%
!! TTAAGAGAGTGTGATTTTGA+AGG + chr5_4:2811969-2811988 Msa0829800:CDS 30.0%
AGAGTTTAAATCACAAGAGA+AGG + chr5_4:2812085-2812104 Msa0829800:CDS 30.0%
TTGATTCAAACTTCTTCACT+CGG - chr5_4:2812331-2812350 None:intergenic 30.0%
GAAGTTTGAATCAAAAGAAG+AGG + chr5_4:2812337-2812356 Msa0829800:CDS 30.0%
TTTGAAGAGGAAATTGAATC+AGG + chr5_4:2812404-2812423 Msa0829800:CDS 30.0%
GATGAAATTGCATAATTAGC+TGG - chr5_4:2812510-2812529 None:intergenic 30.0%
GTTCATCAATTTCCTCAATT+GGG - chr5_4:2812587-2812606 None:intergenic 30.0%
AGTTCATCAATTTCCTCAAT+TGG - chr5_4:2812588-2812607 None:intergenic 30.0%
CTTAAACTGAAAGCTTACAT+TGG + chr5_4:2812825-2812844 Msa0829800:CDS 30.0%
CAATGTGAAGAAAGACAAAA+AGG - chr5_4:2813065-2813084 None:intergenic 30.0%
TTCTTATCACTTCTAAGTGT+AGG + chr5_4:2813101-2813120 Msa0829800:intron 30.0%
TCAATTAAGGACGAATCATT+TGG + chr5_4:2813373-2813392 Msa0829800:intron 30.0%
CAATTAAGGACGAATCATTT+GGG + chr5_4:2813374-2813393 Msa0829800:intron 30.0%
ATATAAGAAAAGCTTGACCA+AGG - chr5_4:2813432-2813451 None:intergenic 30.0%
! GTCAAGCTTTTCTTATATCT+TGG + chr5_4:2813434-2813453 Msa0829800:intron 30.0%
!!! TTTTTTTGGTCTTAACACTC+CGG - chr5_4:2813498-2813517 None:intergenic 30.0%
!!! GCACATATGCATAGTTTTTT+TGG - chr5_4:2813512-2813531 None:intergenic 30.0%
!!! GCTGTTTTTTTAATGGTTTG+AGG + chr5_4:2813530-2813549 Msa0829800:intron 30.0%
ACTTCATCAAACTAACATAC+GGG - chr5_4:2813598-2813617 None:intergenic 30.0%
CACTTCATCAAACTAACATA+CGG - chr5_4:2813599-2813618 None:intergenic 30.0%
!! ATCAACTGATTGATTTTTGG+TGG - chr5_4:2813712-2813731 None:intergenic 30.0%
ATCAATCAGTTGATCTATTG+CGG + chr5_4:2813718-2813737 Msa0829800:CDS 30.0%
! TTCAATTTGAAATCAGGACA+AGG + chr5_4:2813885-2813904 Msa0829800:intron 30.0%
GTTGTCTCATTTACAAACTT+CGG - chr5_4:2814068-2814087 None:intergenic 30.0%
AAGTTTGTAAATGAGACAAC+AGG + chr5_4:2814068-2814087 Msa0829800:three_prime_UTR 30.0%
!!! GCTAACTCTTTTGTTTTTGT+TGG - chr5_4:2814355-2814374 None:intergenic 30.0%
CCAAAGTGAAACACTGTATT+TGG - chr5_4:2810193-2810212 None:intergenic 35.0%
CCAAATACAGTGTTTCACTT+TGG + chr5_4:2810190-2810209 Msa0829800:five_prime_UTR 35.0%
!!! TGAATGGATGCTATTTTCCA+TGG - chr5_4:2810383-2810402 None:intergenic 35.0%
CTATCAATCACAAATCAGCT+AGG - chr5_4:2810454-2810473 None:intergenic 35.0%
GTTCGCTCAAATAGTATCAA+AGG - chr5_4:2810581-2810600 None:intergenic 35.0%
TTTGAGCGAACTTATGAAGT+TGG + chr5_4:2810590-2810609 Msa0829800:intron 35.0%
!!! TCTTTTTTCTTTTTCTGCCC+AGG + chr5_4:2810779-2810798 Msa0829800:intron 35.0%
AAATCACCACTTCAAATCCT+GGG - chr5_4:2810799-2810818 None:intergenic 35.0%
!!! TTTTTTAACCGAAGTTTGCG+AGG - chr5_4:2811034-2811053 None:intergenic 35.0%
ACGAACTAACCAATATTACG+AGG + chr5_4:2811206-2811225 Msa0829800:CDS 35.0%
TCTGCATTTCCTCGTAATAT+TGG - chr5_4:2811218-2811237 None:intergenic 35.0%
TAATCAAACCCTCGATGAAA+CGG - chr5_4:2811290-2811309 None:intergenic 35.0%
! CTTTTTCCTTCAATCTTCCA+CGG - chr5_4:2811383-2811402 None:intergenic 35.0%
TCAGGAAATTAAGACGAGAA+AGG + chr5_4:2811440-2811459 Msa0829800:CDS 35.0%
TCAACGGAAAAGCAATTTGA+AGG + chr5_4:2811591-2811610 Msa0829800:CDS 35.0%
AAAGAAGCAATTTGAAAGCC+AGG + chr5_4:2811764-2811783 Msa0829800:CDS 35.0%
TCAAAGACAAGTAGATGATC+TGG + chr5_4:2811818-2811837 Msa0829800:CDS 35.0%
!! CATTTTGAAAGCCAACTGAA+GGG + chr5_4:2811855-2811874 Msa0829800:CDS 35.0%
GATATTTGAAAGACGGATGA+AGG + chr5_4:2811896-2811915 Msa0829800:CDS 35.0%
TCAAAGAGAGAGGAATTTGA+AGG + chr5_4:2812053-2812072 Msa0829800:CDS 35.0%
GTGATTTAAACTCTTCCACT+CGG - chr5_4:2812079-2812098 None:intergenic 35.0%
TTGATTCAAACCCCTTTACT+CGG - chr5_4:2812121-2812140 None:intergenic 35.0%
GGGGTTTGAATCAAAAAAGA+AGG + chr5_4:2812127-2812146 Msa0829800:CDS 35.0%
! GAAGTTTGAAACAGTAGAGA+AGG + chr5_4:2812169-2812188 Msa0829800:CDS 35.0%
GAAGTTCAAATCAGTAGAGA+AGG + chr5_4:2812211-2812230 Msa0829800:CDS 35.0%
CTGATTCAAACTTCCTAACT+CGG - chr5_4:2812247-2812266 None:intergenic 35.0%
GAAGTTTGAATCAGTAGAGA+AGG + chr5_4:2812253-2812272 Msa0829800:CDS 35.0%
CTGATTCAAACTTCCTAACT+CGG - chr5_4:2812247-2812266 None:intergenic 35.0%
ATTGATGAACTTGAGTCACA+CGG + chr5_4:2812597-2812616 Msa0829800:CDS 35.0%
TTGCTCCTTCTGTCAATTTA+TGG + chr5_4:2812885-2812904 Msa0829800:CDS 35.0%
CCAATCCATAAATTGACAGA+AGG - chr5_4:2812893-2812912 None:intergenic 35.0%
CCTTCTGTCAATTTATGGAT+TGG + chr5_4:2812890-2812909 Msa0829800:CDS 35.0%
AAGATATCTGTAGAGCTGTT+TGG + chr5_4:2812969-2812988 Msa0829800:CDS 35.0%
AGATATCTGTAGAGCTGTTT+GGG + chr5_4:2812970-2812989 Msa0829800:CDS 35.0%
TAAGTGTAGGTTACATCAAG+TGG + chr5_4:2813114-2813133 Msa0829800:intron 35.0%
! TACCAAAACCCTTTTCTCTT+AGG + chr5_4:2813470-2813489 Msa0829800:intron 35.0%
! TTCCTAAGAGAAAAGGGTTT+TGG - chr5_4:2813475-2813494 None:intergenic 35.0%
GATTCTGAACTAAATCTGCA+AGG - chr5_4:2813635-2813654 None:intergenic 35.0%
TCAATCAGTTGATCTATTGC+GGG + chr5_4:2813719-2813738 Msa0829800:CDS 35.0%
! ATTTTATGAGAGCAGTTGCA+AGG + chr5_4:2813764-2813783 Msa0829800:CDS 35.0%
AACCAACTCCATTGAAATCA+AGG + chr5_4:2813788-2813807 Msa0829800:CDS 35.0%
AACCTTGATTTCAATGGAGT+TGG - chr5_4:2813793-2813812 None:intergenic 35.0%
CTTGAGCTAAATAGAGAGAA+AGG + chr5_4:2814009-2814028 Msa0829800:CDS 35.0%
!! TAGCTTTGGTATTGGAAGTA+GGG + chr5_4:2814036-2814055 Msa0829800:exon 35.0%
AGGATTCTGCGTTAAGATTT+GGG + chr5_4:2814088-2814107 Msa0829800:three_prime_UTR 35.0%
! AAGATTTGGGATTCAGTTCT+TGG + chr5_4:2814101-2814120 Msa0829800:three_prime_UTR 35.0%
ATTTATCTGATCGAGACACA+AGG + chr5_4:2814206-2814225 Msa0829800:three_prime_UTR 35.0%
TTTATCTGATCGAGACACAA+GGG + chr5_4:2814207-2814226 Msa0829800:three_prime_UTR 35.0%
CATCAAAATGGTACAATACG+AGG - chr5_4:2814296-2814315 None:intergenic 35.0%
GGAGAGTGAACTAAGAAAGT+AGG - chr5_4:2810280-2810299 None:intergenic 40.0%
CAAATCACCACTTCAAATCC+TGG - chr5_4:2810800-2810819 None:intergenic 40.0%
GACAATGACTTTGCTCTCAA+AGG - chr5_4:2810910-2810929 None:intergenic 40.0%
GAGGAAATGCTGTCATCAAA+AGG - chr5_4:2811015-2811034 None:intergenic 40.0%
GCTCTTCTTCATGAGCTTAA+TGG - chr5_4:2811076-2811095 None:intergenic 40.0%
! CTCATGAAGAAGAGCATACT+CGG + chr5_4:2811081-2811100 Msa0829800:CDS 40.0%
! AGAAGAGCATACTCGGATTA+AGG + chr5_4:2811088-2811107 Msa0829800:CDS 40.0%
ATCCCTTGAGAGAGATATTG+AGG + chr5_4:2811134-2811153 Msa0829800:CDS 40.0%
CTCCTCAATATCTCTCTCAA+GGG - chr5_4:2811139-2811158 None:intergenic 40.0%
ACTCCTCAATATCTCTCTCA+AGG - chr5_4:2811140-2811159 None:intergenic 40.0%
GCCATGGATAGCAATAGTAA+AGG + chr5_4:2811357-2811376 Msa0829800:CDS 40.0%
GCCTTTACTATTGCTATCCA+TGG - chr5_4:2811361-2811380 None:intergenic 40.0%
! TGGTCAGCTTGTTCAAATGA+AGG + chr5_4:2811560-2811579 Msa0829800:CDS 40.0%
GCTTGTTCAAATGAAGGACT+TGG + chr5_4:2811566-2811585 Msa0829800:CDS 40.0%
AATGAAGGACTTGGAGTCAA+CGG + chr5_4:2811575-2811594 Msa0829800:CDS 40.0%
ACGGAAAAGCAATTTGAAGG+TGG + chr5_4:2811594-2811613 Msa0829800:CDS 40.0%
GCAATTTGAAAGCCAGGAAA+AGG + chr5_4:2811770-2811789 Msa0829800:CDS 40.0%
! AGATGATCTGGAATCATGCA+AGG + chr5_4:2811830-2811849 Msa0829800:CDS 40.0%
!! GCATTTTGAAAGCCAACTGA+AGG + chr5_4:2811854-2811873 Msa0829800:CDS 40.0%
! TTGATTTGAGTCCCTTCAGT+TGG - chr5_4:2811869-2811888 None:intergenic 40.0%
GAAGGAGTTGAAGTCAAAAG+AGG + chr5_4:2811914-2811933 Msa0829800:CDS 40.0%
! ACGTTTTCAAAGACGAGTGA+AGG + chr5_4:2811938-2811957 Msa0829800:CDS 40.0%
GCAATATGAAGGACAAGTGA+AGG + chr5_4:2812022-2812041 Msa0829800:CDS 40.0%
GGAGTTACAATCAAAGAGAG+AGG + chr5_4:2812043-2812062 Msa0829800:CDS 40.0%
! GACTTTGAAAGCCGAGTAAA+GGG + chr5_4:2812107-2812126 Msa0829800:CDS 40.0%
! ACTTTGAAAGCCGAGTAAAG+GGG + chr5_4:2812108-2812127 Msa0829800:CDS 40.0%
CTGTTTCAAACTTCGTCACT+CGG - chr5_4:2812163-2812182 None:intergenic 40.0%
! CTGATTTGAACTTCCTCACT+CGG - chr5_4:2812205-2812224 None:intergenic 40.0%
GAAGTTTGAATCAGCAGAGA+AGG + chr5_4:2812295-2812314 Msa0829800:CDS 40.0%
AAACTTCTTCCATCAATGGC+AGG - chr5_4:2812555-2812574 None:intergenic 40.0%
CTGCAAACTTCTTCCATCAA+TGG - chr5_4:2812559-2812578 None:intergenic 40.0%
TTTGCAGTTTCTCCCAATTG+AGG + chr5_4:2812572-2812591 Msa0829800:CDS 40.0%
! ACATTTTCTCCCTTACACTG+TGG - chr5_4:2812705-2812724 None:intergenic 40.0%
GGTTTGGCACATAAAGTTTC+TGG + chr5_4:2812999-2813018 Msa0829800:CDS 40.0%
ATCTTGGCAAGAACAATCCT+TGG + chr5_4:2813412-2813431 Msa0829800:intron 40.0%
CTTATATCTTGGTCGAACTC+TGG + chr5_4:2813445-2813464 Msa0829800:intron 40.0%
! AACCCTTTTCTCTTAGGAAC+CGG + chr5_4:2813476-2813495 Msa0829800:intron 40.0%
TTCTCAGACTAGCAATTTCC+TGG - chr5_4:2813918-2813937 None:intergenic 40.0%
!! GTAGCTTTGGTATTGGAAGT+AGG + chr5_4:2814035-2814054 Msa0829800:exon 40.0%
CAGGATTCTGCGTTAAGATT+TGG + chr5_4:2814087-2814106 Msa0829800:three_prime_UTR 40.0%
TTATCTGATCGAGACACAAG+GGG + chr5_4:2814208-2814227 Msa0829800:three_prime_UTR 40.0%
AGGATGACAGTAATGAGAAC+TGG + chr5_4:2814232-2814251 Msa0829800:three_prime_UTR 40.0%
TACTTACCTTTGTGTGGCTG+AGG - chr5_4:2810301-2810320 None:intergenic 45.0%
TTTCTGCCCAGGATTTGAAG+TGG + chr5_4:2810790-2810809 Msa0829800:intron 45.0%
TCATCAAAAGGCCTCTTTGC+TGG - chr5_4:2811003-2811022 None:intergenic 45.0%
CAGCATTTCCTCGCAAACTT+CGG + chr5_4:2811023-2811042 Msa0829800:CDS 45.0%
GAGACAACTTCTTACAGCCA+TGG + chr5_4:2811341-2811360 Msa0829800:CDS 45.0%
GATAGCAATAGTAAAGGCCG+TGG + chr5_4:2811363-2811382 Msa0829800:CDS 45.0%
TAAAGGCCGTGGAAGATTGA+AGG + chr5_4:2811374-2811393 Msa0829800:CDS 45.0%
GTAATGTCAGAGAGGAGAAC+TGG + chr5_4:2811540-2811559 Msa0829800:CDS 45.0%
! TCGGTCACACTGCTTCATTT+TGG - chr5_4:2811637-2811656 None:intergenic 45.0%
ATTCAACCCCTTCTAGTCGT+CGG - chr5_4:2811656-2811675 None:intergenic 45.0%
GCTCTATAAAGGGCAGACAA+AGG + chr5_4:2811686-2811705 Msa0829800:CDS 45.0%
! TAAAGGGCAGACAAAGGTGT+CGG + chr5_4:2811692-2811711 Msa0829800:CDS 45.0%
GGAATTCGAAAGTCAGGTGA+AGG + chr5_4:2811728-2811747 Msa0829800:CDS 45.0%
! TTAATCCGAGCACCTTTTCC+TGG - chr5_4:2811785-2811804 None:intergenic 45.0%
!! AGAGTGTGATTTTGAAGGCC+AGG + chr5_4:2811974-2811993 Msa0829800:CDS 45.0%
!! TGATTTTGAAGGCCAGGTGA+AGG + chr5_4:2811980-2811999 Msa0829800:CDS 45.0%
! TTGATTCGAAGTCCTTCACC+TGG - chr5_4:2811995-2812014 None:intergenic 45.0%
! GGACTTTGAAAGCCGAGTAA+AGG + chr5_4:2812106-2812125 Msa0829800:CDS 45.0%
!! AGGACTTTGAAATCCGAGTG+AGG + chr5_4:2812189-2812208 Msa0829800:CDS 45.0%
! AGGACTTTGAAAGCCGAGTT+AGG + chr5_4:2812231-2812250 Msa0829800:CDS 45.0%
! AGGACTTTGAAAGCCGAGTT+AGG + chr5_4:2812231-2812250 Msa0829800:CDS 45.0%
GAGGAACTTGAGCTAAGAGA+CGG + chr5_4:2812356-2812375 Msa0829800:CDS 45.0%
CCTTACACTGTGGAATGATG+GGG - chr5_4:2812695-2812714 None:intergenic 45.0%
CCCTTACACTGTGGAATGAT+GGG - chr5_4:2812696-2812715 None:intergenic 45.0%
CCCATCATTCCACAGTGTAA+GGG + chr5_4:2812693-2812712 Msa0829800:CDS 45.0%
TCCCTTACACTGTGGAATGA+TGG - chr5_4:2812697-2812716 None:intergenic 45.0%
GTTGTTCGAGCAGATCAATG+TGG - chr5_4:2812746-2812765 None:intergenic 45.0%
CGCACATGAGGTTTAACATG+TGG - chr5_4:2812783-2812802 None:intergenic 45.0%
ATTGCTTCTTCTCGCACATG+AGG - chr5_4:2812795-2812814 None:intergenic 45.0%
TGCAAATCCAGTACCAGTTC+TGG + chr5_4:2812857-2812876 Msa0829800:CDS 45.0%
GCAAATCCAGTACCAGTTCT+GGG + chr5_4:2812858-2812877 Msa0829800:CDS 45.0%
CAAATCCAGTACCAGTTCTG+GGG + chr5_4:2812859-2812878 Msa0829800:CDS 45.0%
AAAAACCCCAGAACTGGTAC+TGG - chr5_4:2812867-2812886 None:intergenic 45.0%
! TGTAGAGCTGTTTGGGACTA+TGG + chr5_4:2812977-2812996 Msa0829800:CDS 45.0%
! GTAGAGCTGTTTGGGACTAT+GGG + chr5_4:2812978-2812997 Msa0829800:CDS 45.0%
! GAGCATTCGAGTTCGAATCT+TGG + chr5_4:2813396-2813415 Msa0829800:intron 45.0%
CTCCGGTTCCTAAGAGAAAA+GGG - chr5_4:2813481-2813500 None:intergenic 45.0%
ACTCCGGTTCCTAAGAGAAA+AGG - chr5_4:2813482-2813501 None:intergenic 45.0%
GAACTAAATCTGCAAGGTGC+AGG - chr5_4:2813629-2813648 None:intergenic 45.0%
AACATGCAGGTGCATGAAGA+TGG - chr5_4:2813852-2813871 None:intergenic 45.0%
!! AAGGCGAGTAGCTTTGGTAT+TGG + chr5_4:2814028-2814047 Msa0829800:exon 45.0%
! CTTTGGTATTGGAAGTAGGG+CGG + chr5_4:2814039-2814058 Msa0829800:three_prime_UTR 45.0%
TGGGATTCAGTTCTTGGATG+TGG + chr5_4:2814107-2814126 Msa0829800:three_prime_UTR 45.0%
GGGATTCAGTTCTTGGATGT+GGG + chr5_4:2814108-2814127 Msa0829800:three_prime_UTR 45.0%
!! TAAATGATAAAAAAAAAAAA+GGG - chr5_4:2810355-2810374 None:intergenic 5.0%
CTCTTTGCTGGCAGTCTAGT+AGG - chr5_4:2810991-2811010 None:intergenic 50.0%
CTACTAGACTGCCAGCAAAG+AGG + chr5_4:2810989-2811008 Msa0829800:CDS 50.0%
!! ACTCGGATTAAGGAGCGTTG+AGG + chr5_4:2811098-2811117 Msa0829800:CDS 50.0%
AGTGTGACCGACGACTAGAA+GGG + chr5_4:2811646-2811665 Msa0829800:CDS 50.0%
!! AAAGGTGTCGGAGTCAAAGC+AGG + chr5_4:2811704-2811723 Msa0829800:CDS 50.0%
GCAGGAGGAATTCGAAAGTC+AGG + chr5_4:2811722-2811741 Msa0829800:CDS 50.0%
!! GAAAGCCAGGAAAAGGTGCT+CGG + chr5_4:2811777-2811796 Msa0829800:CDS 50.0%
AGAGGAATTTGAAGGCCGAG+TGG + chr5_4:2812061-2812080 Msa0829800:CDS 50.0%
CAACCAAGTCCTGCCATTGA+TGG + chr5_4:2812543-2812562 Msa0829800:CDS 50.0%
CTTCCATCAATGGCAGGACT+TGG - chr5_4:2812549-2812568 None:intergenic 50.0%
! ACTGTGGAATGATGGGGTTC+TGG - chr5_4:2812689-2812708 None:intergenic 50.0%
CCCCATCATTCCACAGTGTA+AGG + chr5_4:2812692-2812711 Msa0829800:CDS 50.0%
AGGAGCAAAAACCCCAGAAC+TGG - chr5_4:2812873-2812892 None:intergenic 50.0%
! GCTGTTTGGGACTATGGGTT+TGG + chr5_4:2812983-2813002 Msa0829800:CDS 50.0%
GAGAGAAAGGCGAGTAGCTT+TGG + chr5_4:2814022-2814041 Msa0829800:exon 50.0%
CACTCTCCTCAGCCACACAA+AGG + chr5_4:2810292-2810311 Msa0829800:five_prime_UTR 55.0%
!! CGGATTAAGGAGCGTTGAGG+AGG + chr5_4:2811101-2811120 Msa0829800:CDS 55.0%
AGCGCATGTCCGTTTCATCG+AGG + chr5_4:2811278-2811297 Msa0829800:CDS 55.0%
GCGCATGTCCGTTTCATCGA+GGG + chr5_4:2811279-2811298 Msa0829800:CDS 55.0%
CAGTGTGACCGACGACTAGA+AGG + chr5_4:2811645-2811664 Msa0829800:CDS 55.0%
GTGTGACCGACGACTAGAAG+GGG + chr5_4:2811647-2811666 Msa0829800:CDS 55.0%
TCAGGACAAGGCAAGAGACC+AGG + chr5_4:2813897-2813916 Msa0829800:intron 55.0%
CTGATCGAGACACAAGGGGA+AGG + chr5_4:2814212-2814231 Msa0829800:three_prime_UTR 55.0%
! GGTGTCGGAGTCAAAGCAGG+AGG + chr5_4:2811707-2811726 Msa0829800:CDS 60.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 2810189 2814418 2810189 ID=Msa0829800;Name=Msa0829800
chr5_4 mRNA 2810189 2814418 2810189 ID=Msa0829800-mRNA-1;Parent=Msa0829800;Name=Msa0829800-mRNA-1;_AED=0.11;_eAED=0.11;_QI=156|0.75|0.8|1|0.75|0.6|5|380|803
chr5_4 exon 2810189 2810313 2810189 ID=Msa0829800-mRNA-1:exon:983;Parent=Msa0829800-mRNA-1
chr5_4 exon 2810801 2812425 2810801 ID=Msa0829800-mRNA-1:exon:984;Parent=Msa0829800-mRNA-1
chr5_4 exon 2812511 2813020 2812511 ID=Msa0829800-mRNA-1:exon:985;Parent=Msa0829800-mRNA-1
chr5_4 exon 2813640 2813809 2813640 ID=Msa0829800-mRNA-1:exon:986;Parent=Msa0829800-mRNA-1
chr5_4 exon 2813901 2814418 2813901 ID=Msa0829800-mRNA-1:exon:987;Parent=Msa0829800-mRNA-1
chr5_4 five_prime_UTR 2810189 2810313 2810189 ID=Msa0829800-mRNA-1:five_prime_utr;Parent=Msa0829800-mRNA-1
chr5_4 five_prime_UTR 2810801 2810831 2810801 ID=Msa0829800-mRNA-1:five_prime_utr;Parent=Msa0829800-mRNA-1
chr5_4 CDS 2810832 2812425 2810832 ID=Msa0829800-mRNA-1:cds;Parent=Msa0829800-mRNA-1
chr5_4 CDS 2812511 2813020 2812511 ID=Msa0829800-mRNA-1:cds;Parent=Msa0829800-mRNA-1
chr5_4 CDS 2813640 2813809 2813640 ID=Msa0829800-mRNA-1:cds;Parent=Msa0829800-mRNA-1
chr5_4 CDS 2813901 2814038 2813901 ID=Msa0829800-mRNA-1:cds;Parent=Msa0829800-mRNA-1
chr5_4 three_prime_UTR 2814039 2814418 2814039 ID=Msa0829800-mRNA-1:three_prime_utr;Parent=Msa0829800-mRNA-1
Gene Sequence

>Msa0829800

ATGGTTGTTAGGGCTTCTAAAAGAAAAAAGTTGCTGAATTTATATGACTCTAAGTCTGATGATGATTATGATGTACCTTTGAGAGCAAAGTCATTGTCAACTTTGAATTTGGGAGCAAGTAGTAGTAGTAGTAGTAGTAAATCTATTGAGAATAGGCCTACTAGACTGCCAGCAAAGAGGCCTTTTGATGACAGCATTTCCTCGCAAACTTCGGTTAAAAAATCAAAGTTGTCATCGTTTTCCATTAAGCTCATGAAGAAGAGCATACTCGGATTAAGGAGCGTTGAGGAGGAAAAATTGCAATCCCTTGAGAGAGATATTGAGGAGTGCTCCAAAGAGCTTCTAAATAAGAAAAAGCAAGCTAGTGATGTTAGACGAACTAACCAATATTACGAGGAAATGCAGAACAAAATCGAAAAAGGTGTCAAAGATCTTGCAGCAAACGAAGCGCATGTCCGTTTCATCGAGGGTTTGATTAAAAACAAAACACTTGAGCTCAAGAAAAATAAGAGACAACTTCTTACAGCCATGGATAGCAATAGTAAAGGCCGTGGAAGATTGAAGGAAAAAGAGCTAGAGACTCTTTCACAAAAAATTGCTGAATGTACTCAGGAAATTAAGACGAGAAAGGAAGAGCTTGATGCTTCGAAAATATCTGTTAATCATAAAATAAAAGAACTAATGTCAGAAAGGAGTAACATACTAAATGTAATGTCAGAGAGGAGAACTGGTCAGCTTGTTCAAATGAAGGACTTGGAGTCAACGGAAAAGCAATTTGAAGGTGGAGCTACAGAGTTTGATTCCAAAATGAAGCAGTGTGACCGACGACTAGAAGGGGTTGAATCAAATGAGAAGCTCTATAAAGGGCAGACAAAGGTGTCGGAGTCAAAGCAGGAGGAATTCGAAAGTCAGGTGAAGGAGCTTGAATCAAAAAAGAAGCAATTTGAAAGCCAGGAAAAGGTGCTCGGATTAAAAGAGAAGCTATTTCAAAGACAAGTAGATGATCTGGAATCATGCAAGGAGCATTTTGAAAGCCAACTGAAGGGACTCAAATCAAAAGAGAAGATATTTGAAAGACGGATGAAGGAGTTGAAGTCAAAAGAGGAACGTTTTCAAAGACGAGTGAAGGAGTTTGAATTAAGAGAGTGTGATTTTGAAGGCCAGGTGAAGGACTTCGAATCAAAATTGAAGCAATATGAAGGACAAGTGAAGGAGTTACAATCAAAGAGAGAGGAATTTGAAGGCCGAGTGGAAGAGTTTAAATCACAAGAGAAGGACTTTGAAAGCCGAGTAAAGGGGTTTGAATCAAAAAAGAAGGACTTTGAAAGCCGAGTGACGAAGTTTGAAACAGTAGAGAAGGACTTTGAAATCCGAGTGAGGAAGTTCAAATCAGTAGAGAAGGACTTTGAAAGCCGAGTTAGGAAGTTTGAATCAGTAGAGAAGGACTTTGAAAGCCGAGTTAGGAAGTTTGAATCAGCAGAGAAGGACTTTGAAAGCCGAGTGAAGAAGTTTGAATCAAAAGAAGAGGAACTTGAGCTAAGAGACGGTCAATATGAAACGTTAATAAAATCATTTGAAGAGGAAATTGAATCAGCTAATTATGCAATTTCATCTTTCACAGATGATCAACCAAGTCCTGCCATTGATGGAAGAAGTTTGCAGTTTCTCCCAATTGAGGAAATTGATGAACTTGAGTCACACGGCAATGACAGTCTAGCTAATCTGCTAGCATCATCATCAGACCCATCAAAAGATGTTTTAGATATAATCCAGAACCCCATCATTCCACAGTGTAAGGGAGAAAATGTTGTAATTATTGATGATCACCACATTGATCTGCTCGAACAACTGATGAGAATCTCACCACATGTTAAACCTCATGTGCGAGAAGAAGCAATGAAGCTAGCACTTAAACTGAAAGCTTACATTGGAGAAAATACTGCAAATCCAGTACCAGTTCTGGGGTTTTTGCTCCTTCTGTCAATTTATGGATTGGTTTCTTCTTTCGATGAAGATGAAGTTTTAAAGCTTTTTGGGTTTGCTGCTCAACACAAGATATCTGTAGAGCTGTTTGGGACTATGGGTTTGGCACATAAAGTTTCTGATTTAGTTCAGAATCTTATCATGAAGCAGCAATATATTGAAGCTGTTAGATTCATTTGTTATAACACGGCCACCAAAAATCAATCAGTTGATCTATTGCGGGAACATGTGCAGAATGCAAGAACATTTTATGAGAGCAGTTGCAAGGCAACCAACTCCATTGAAATCAAGGACAAGGCAAGAGACCAGGAAATTGCTAGTCTGAGAACTGTTCTACAGTGTCTTTCAGATAACAATATTGAATCTGCGGATTTACTCAACGAGATTCACAGTAGAATTCTTGAGCTAAATAGAGAGAAAGGCGAGTAG
Protein sequence

>Msa0829800

MVVRASKRKKLLNLYDSKSDDDYDVPLRAKSLSTLNLGASSSSSSSKSIENRPTRLPAKRPFDDSISSQTSVKKSKLSSFSIKLMKKSILGLRSVEEEKLQSLERDIEECSKELLNKKKQASDVRRTNQYYEEMQNKIEKGVKDLAANEAHVRFIEGLIKNKTLELKKNKRQLLTAMDSNSKGRGRLKEKELETLSQKIAECTQEIKTRKEELDASKISVNHKIKELMSERSNILNVMSERRTGQLVQMKDLESTEKQFEGGATEFDSKMKQCDRRLEGVESNEKLYKGQTKVSESKQEEFESQVKELESKKKQFESQEKVLGLKEKLFQRQVDDLESCKEHFESQLKGLKSKEKIFERRMKELKSKEERFQRRVKEFELRECDFEGQVKDFESKLKQYEGQVKELQSKREEFEGRVEEFKSQEKDFESRVKGFESKKKDFESRVTKFETVEKDFEIRVRKFKSVEKDFESRVRKFESVEKDFESRVRKFESAEKDFESRVKKFESKEEELELRDGQYETLIKSFEEEIESANYAISSFTDDQPSPAIDGRSLQFLPIEEIDELESHGNDSLANLLASSSDPSKDVLDIIQNPIIPQCKGENVVIIDDHHIDLLEQLMRISPHVKPHVREEAMKLALKLKAYIGENTANPVPVLGFLLLLSIYGLVSSFDEDEVLKLFGFAAQHKISVELFGTMGLAHKVSDLVQNLIMKQQYIEAVRFICYNTATKNQSVDLLREHVQNARTFYESSCKATNSIEIKDKARDQEIASLRTVLQCLSDNNIESADLLNEIHSRILELNREKGE*