AlfalfaGEDB | Msa0311280

M. sativa cultivar zhongmu-4 / Msa0311280

KEGG: K10352
GO: -
PFAM: PF07899.14

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0311280	RHN71744.1	89.238	827	54	5	1	801	1	818	0.0	1396

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0311280	sp\|Q5XV31\|FRL5_ARATH	27.003	337	195	14	439	753	88	395	4.28e-11	70.5

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000190	Msa0311280	0.801216	9.997372e-49	-8.615850e-47
Msa0017510	Msa0311280	0.841317	5.112296e-58	-8.615850e-47
Msa0028640	Msa0311280	0.815677	8.176823e-52	-8.615850e-47
Msa0089630	Msa0311280	0.807673	4.506285e-50	-8.615850e-47
Msa0095900	Msa0311280	0.815556	8.702716e-52	-8.615850e-47
Msa0110970	Msa0311280	0.837883	3.996337e-57	-8.615850e-47
Msa0118570	Msa0311280	0.805925	1.054991e-49	-8.615850e-47
Msa0136650	Msa0311280	0.800642	1.309321e-48	-8.615850e-47
Msa0157290	Msa0311280	0.841848	3.705377e-58	-8.615850e-47
Msa0163980	Msa0311280	0.808375	3.194427e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0311280	MtrunA17_Chr2g0280141	89.238	827	54	5	1	801	1	818	0.0	1396
Msa0311280	MtrunA17_Chr2g0325811	44.258	836	366	15	19	799	40	830	0.0	541
Msa0311280	MtrunA17_Chr2g0325711	42.892	816	393	15	10	781	31	817	6.26e-177	529
Msa0311280	MtrunA17_Chr2g0325661	42.873	884	354	19	16	800	45	876	2.38e-164	498
Msa0311280	MtrunA17_Chr2g0325861	42.484	805	303	14	61	725	62	846	1.42e-157	480
Msa0311280	MtrunA17_Chr2g0325361	48.480	592	262	8	247	800	491	1077	4.26e-133	422
Msa0311280	MtrunA17_Chr2g0325361	35.390	551	293	10	25	527	13	548	8.52e-56	208
Msa0311280	MtrunA17_Chr6g0471251	38.601	829	350	21	19	800	51	767	5.75e-128	400
Msa0311280	MtrunA17_Chr2g0325611	51.812	469	185	10	358	800	137	590	1.85e-123	383
Msa0311280	MtrunA17_Chr2g0325611	38.735	253	118	7	187	426	93	321	1.21e-25	112

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0311280	AT5G27220.1	28.079	203	134	7	579	776	872	1067	3.55e-15	80.5
Msa0311280	AT5G27220.1	25.736	645	405	20	75	695	208	802	1.20e-14	79.0
Msa0311280	AT5G27220.3	28.079	203	134	7	579	776	872	1067	3.55e-15	80.5
Msa0311280	AT5G27220.3	25.736	645	405	20	75	695	208	802	1.20e-14	79.0
Msa0311280	AT5G27220.4	25.736	645	405	20	75	695	208	802	7.02e-15	79.7
Msa0311280	AT5G27220.4	27.815	151	101	4	628	776	868	1012	5.10e-12	70.5
Msa0311280	AT5G27220.2	25.736	645	405	20	75	695	208	802	7.02e-15	79.7
Msa0311280	AT5G27220.2	27.815	151	101	4	628	776	868	1012	5.10e-12	70.5
Msa0311280	AT1G31814.1	26.786	224	150	6	567	781	117	335	1.29e-13	74.7
Msa0311280	AT5G48385.1	27.333	150	108	1	653	801	308	457	2.23e-13	74.3

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Find 164 sgRNAs with CRISPR-Local

Find 307 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTTCTTCCATCAATGGC+AGG	0.555447	2_4:+6133753	None:intergenic
AAAGAAGCAATTTGAAAGCC+AGG	0.492303	2_4:-6134538	Msa0311280:CDS
AAAGGTGTCGGAGTCAAAGC+AGG	0.537649	2_4:-6134598	Msa0311280:CDS
AACCAACTCCATTGAAATCA+AGG	0.448934	2_4:-6132517	Msa0311280:intron
AACCTTGATTTCAATGGAGT+TGG	0.432488	2_4:+6132515	None:intergenic
AAGAGAAGATATTTGAAAGA+CGG	0.560688	2_4:-6134413	Msa0311280:CDS
AAGATATCTGTAGAGCTGTT+TGG	0.368664	2_4:-6133336	Msa0311280:CDS
AAGCGAAACACTGTATTTGG+TGG	0.570485	2_4:+6136927	None:intergenic
AAGGCGAGTAGCTTTGGTAT+TGG	0.430765	2_4:-6132277	Msa0311280:exon
AAGTAGGAATGAAATGAGAA+AGG	0.489576	2_4:+6136850	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGTAGGAATGAAATGAGAA+AGG	+	chr2_4:6131987-6132006	None:intergenic	30.0%
AGAATTTACTTACCTTTGTG+TGG	+	chr2_4:6132030-6132049	None:intergenic	30.0%
GTTACAAACGTCAAACTTAA+AGG	+	chr2_4:6132835-6132854	None:intergenic	30.0%
GTTTGACGTTTGTAACATAT+AGG	-	chr2_4:6132166-6132185	Msa0311280:three_prime_UTR	30.0%
AAAAAAACTGGGTGTAGTAA+AGG	+	chr2_4:6132375-6132394	None:intergenic	30.0%
CCTGATTCTTCAAAAAAAAC+TGG	+	chr2_4:6132387-6132406	None:intergenic	30.0%
AATAATGCTGGTATTACACT+TGG	-	chr2_4:6132596-6132615	Msa0311280:CDS	30.0%
GTATGAATAAAAATGGAGTC+AGG	-	chr2_4:6132649-6132668	Msa0311280:CDS	30.0%
GTTACAAACGTCAAACTTAA+AGG	-	chr2_4:6132835-6132854	Msa0311280:intron	30.0%
GTTTGACGTTTGTAACATAT+AGG	+	chr2_4:6132166-6132185	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	6131909	6136947	6131909	ID=Msa0311280;Name=Msa0311280
chr2_4	mRNA	6131909	6136947	6131909	ID=Msa0311280-mRNA-1;Parent=Msa0311280;Name=Msa0311280-mRNA-1;_AED=0.14;_eAED=0.14;_QI=159\|0.75\|0.8\|1\|0.75\|0.6\|5\|380\|801
chr2_4	exon	6131909	6132426	6131909	ID=Msa0311280-mRNA-1:exon:2331;Parent=Msa0311280-mRNA-1
chr2_4	exon	6132518	6132687	6132518	ID=Msa0311280-mRNA-1:exon:2330;Parent=Msa0311280-mRNA-1
chr2_4	exon	6133307	6133816	6133307	ID=Msa0311280-mRNA-1:exon:2329;Parent=Msa0311280-mRNA-1
chr2_4	exon	6133902	6135520	6133902	ID=Msa0311280-mRNA-1:exon:2328;Parent=Msa0311280-mRNA-1
chr2_4	exon	6136820	6136947	6136820	ID=Msa0311280-mRNA-1:exon:2327;Parent=Msa0311280-mRNA-1
chr2_4	five_prime_UTR	6136820	6136947	6136820	ID=Msa0311280-mRNA-1:five_prime_utr;Parent=Msa0311280-mRNA-1
chr2_4	five_prime_UTR	6135490	6135520	6135490	ID=Msa0311280-mRNA-1:five_prime_utr;Parent=Msa0311280-mRNA-1
chr2_4	CDS	6133902	6135489	6133902	ID=Msa0311280-mRNA-1:cds;Parent=Msa0311280-mRNA-1
chr2_4	CDS	6133307	6133816	6133307	ID=Msa0311280-mRNA-1:cds;Parent=Msa0311280-mRNA-1
chr2_4	CDS	6132518	6132687	6132518	ID=Msa0311280-mRNA-1:cds;Parent=Msa0311280-mRNA-1
chr2_4	CDS	6132289	6132426	6132289	ID=Msa0311280-mRNA-1:cds;Parent=Msa0311280-mRNA-1
chr2_4	three_prime_UTR	6131909	6132288	6131909	ID=Msa0311280-mRNA-1:three_prime_utr;Parent=Msa0311280-mRNA-1

Gene Sequence

>Msa0311280

ATGGTTGTTAGGGCTTCTAAAAGAAAAAAGTTGCTGAATTTATATGACTCTAAGTCTGATGATGATTATGATGTACCTTTGAGAGCAAAGTCATTGTCAACTTTGAATTTGGGAGCAAGTAGTAGTAGTAGTAGTAAATCAATTGAGAATAGGCCTACTAGACTGCCAGCAAAGAGGCCTTTTGATGACAACATTTCCTCGCAAACTTCGGTTAAAAAATCAAAGTTGTCATCGTTTTCCATTAAGCTCATGAAGAAGAGCATACTCGGATTAAGGAGCGTTGAGGAGGAAAAATTGCAATCCCTTGAGAGAGATATTGAGGAGTGCTCCAAAGAGCTTCTAAATAAGAAAAAGCAAGCTAGTGATGTTAGACGAACTAACCAATATTACGAGGAAATGCAGAACAAAATCGAAAAAGGTGTCAAAGATCTTGCAGCAAACGAAGCACATGTCCGTTTCATCGAGGGTTTGATTAAAAACAAAACACTTGAGCTCAAGAAAAATAAGAGACAACTTCTTACAGCCATGGATAGCAATAGTAAAGGCCGTGGAAGATTGAAGGAAAAAGAGCTAGAGACTCTTTCACAAAAAATTGCTGAATGTACTCAGGAAATTAAGACGAGAAAGGAAGAGCTTGATGCTTCGAAAATATCTGTTAATCATAAAATAAAAGAACTAATGTCAGAAAGGAGTAACATACTAAATGTAATGTCAGAGAGGAGAACTGGTCAGCTTGTTCAAATGAAGGACTTGGAGTTAACGGAAAAGCAATTTGAAGGTGGAGCTACAGAGTTTGATTCCAAAATGAAGCAGTGTGACCGACGACTAGAAGGGGTTGAATCAAATGAGAAGCTCTATAAAGGGCAGACAAAGGTGTCGGAGTCAAAGCAGGAGGAATTCGAAAGTCAGGTGAAGGAGCTTGAATCAAAAAAGAAGCAATTTGAAAGCCAGGAAAAGGTGCTCGGATTAAAAGAGAAGCTATTTCAAAGACAAGTAGATGATCTGGAATCATGCAAGGAGCATTTTGAAAGCCAACTGAAGGGACTCAAATCAAAAGAGAAGATATTTGAAAGACGGATGAAGGAGTTGAAGTCAAAAGAGGAACGTTTTCAAAGACGAGTGAAGGAGTTTGAATTAAGAGAGTGTGATTTTGAAGGCCAGGTGAAGGACTTCGAATCAAAATTGAAGCAATATGAAGGACAAGTGAAGGAGTTACAATCAAAGAGAGAGGAATTTGAAGGCCGAGTGGAAGAGTTTAAATCACAAGAGAAGGACTTTGAAAGCCGAGTAAAGGGGTTTGAATCAAAAAAGGACTTTGAAAGCCGAGTGACGAAGTTTGAAACAGTAGAGAAGGACTTTGAAATCCGAGTGAGGAAGTTCAAATCAGTAGAGAAGGACTTTGAAAGCCGAGTTAGGAAGTTTGAATCAGTAGAGAAGGACTTTGAAAGCCGAGTTAGGAAGTTTGAATCAGCAGAGAAGGACTTTGAAAGCCGAGTGAAGAAGTTTGAATCAAAAGAAGAGGAACTTGAGCTAAGAGACGGTCAATATGAAACGTTAATAAAATCATTTGAAGAGGAAATTGAATCAGCTAATTATGCAATTTCATCTTTCACAGATGATCAACCAAGTCCTGCCATTGATGGAAGAAGTTTGCAGTTTCTCCCAATTGAGGAAATTGATGAACTTGAGTCACACGGCAATGACAGTCTAGCTAATCTGCTGGCATCATCATCAGACCCATCAAAAGATGTTTTAGATATAATCCAGAACCCCATCATTCCACAGTGTAAGGGAGAAAATGTTGTAATTATTGATGATCACCACATTGATCTGCTCGAACAACTGATGAGAATCTCACCACATGTTAAACCTCATGTGCGAGAAGAAGCAATGAAGCTAGCACTTAAACTGAAAGCTTACATTGGAGAAAATACTGCAAATCCAGTACCAGTTCTGGGGTTTTTGCTCCTTCTGTCAATTTATGGATTGGTTTCTTCTTTCGATGAAGATGAAGTTTTAAAGCTTTTTGGGTTTGCTGCTCAACACAAGATATCTGTAGAGCTGTTTGGGACTATGGGTTTGGCACATAAAGTTTCTGATTTAGTTCAGAATCTTATCATGAAGCAGCAATATATTGAAGCTGTTAGATTCATTTGTTATAACACGGCCACCAAGAATCAATCAGTTGATCTATTGCGGGAACATGTGCAGAATGCAAGAACAATTTATGAGAGCAGTTGCAAGGCAACCAACTCCATTGAAATCAAGGACAAGGCAAGAGACCAGGAAATTGCTAGTCTGAGAACTGTTCTACAGTGTCTTTCAGATAACAATATTGAATCTGCGGATTTACTCAACGAGATTCACAGTAGAATTCTTGAGCTAAATAGAGAGAAAGGCGAGTAG

Protein sequence

>Msa0311280

MVVRASKRKKLLNLYDSKSDDDYDVPLRAKSLSTLNLGASSSSSSKSIENRPTRLPAKRPFDDNISSQTSVKKSKLSSFSIKLMKKSILGLRSVEEEKLQSLERDIEECSKELLNKKKQASDVRRTNQYYEEMQNKIEKGVKDLAANEAHVRFIEGLIKNKTLELKKNKRQLLTAMDSNSKGRGRLKEKELETLSQKIAECTQEIKTRKEELDASKISVNHKIKELMSERSNILNVMSERRTGQLVQMKDLELTEKQFEGGATEFDSKMKQCDRRLEGVESNEKLYKGQTKVSESKQEEFESQVKELESKKKQFESQEKVLGLKEKLFQRQVDDLESCKEHFESQLKGLKSKEKIFERRMKELKSKEERFQRRVKEFELRECDFEGQVKDFESKLKQYEGQVKELQSKREEFEGRVEEFKSQEKDFESRVKGFESKKDFESRVTKFETVEKDFEIRVRKFKSVEKDFESRVRKFESVEKDFESRVRKFESAEKDFESRVKKFESKEEELELRDGQYETLIKSFEEEIESANYAISSFTDDQPSPAIDGRSLQFLPIEEIDELESHGNDSLANLLASSSDPSKDVLDIIQNPIIPQCKGENVVIIDDHHIDLLEQLMRISPHVKPHVREEAMKLALKLKAYIGENTANPVPVLGFLLLLSIYGLVSSFDEDEVLKLFGFAAQHKISVELFGTMGLAHKVSDLVQNLIMKQQYIEAVRFICYNTATKNQSVDLLREHVQNARTIYESSCKATNSIEIKDKARDQEIASLRTVLQCLSDNNIESADLLNEIHSRILELNREKGE*