AlfalfaGEDB | Msa0277850

M. sativa cultivar zhongmu-4 / Msa0277850

KEGG: K19307
GO: -
PFAM: PF10354.12

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0277850	XP_003594344.1	92.771	249	17	1	9	256	1	249	4.63e-165	466

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0277850	sp\|P0C8L4\|Y4648_ARATH	48.529	204	101	3	46	246	4	206	6.74e-58	186

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0277850	A2Q5H3	92.771	249	17	1	9	256	1	249	2.21e-165	466

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0001450	Msa0277850	0.805173	1.517094e-49	-8.615850e-47
Msa0030170	Msa0277850	0.824347	8.477940e-54	-8.615850e-47
Msa0063890	Msa0277850	0.818776	1.642932e-52	-8.615850e-47
Msa0101440	Msa0277850	0.820430	6.887218e-53	-8.615850e-47
Msa0125660	Msa0277850	0.808792	2.601627e-50	-8.615850e-47
Msa0173890	Msa0277850	0.800083	1.702094e-48	-8.615850e-47
Msa0195630	Msa0277850	0.809975	1.450873e-50	-8.615850e-47
Msa0197690	Msa0277850	0.834886	2.312154e-56	-8.615850e-47
Msa0204960	Msa0277850	0.804043	2.611018e-49	-8.615850e-47
Msa0211150	Msa0277850	0.824800	6.633597e-54	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0277850	MtrunA17_Chr2g0290801	92.771	249	17	1	9	256	1	249	4.26e-169	466
Msa0277850	MtrunA17_Chr5g0444781	51.759	199	95	1	48	246	8	205	7.77e-70	217
Msa0277850	MtrunA17_Chr5g0444781	64.706	51	18	0	48	98	257	307	1.55e-13	69.3
Msa0277850	MtrunA17_Chr6g0483551	48.500	200	101	2	48	246	4	202	1.50e-61	192
Msa0277850	MtrunA17_Chr6g0483591	49.231	195	98	1	52	246	8	201	3.19e-61	192
Msa0277850	MtrunA17_Chr6g0483571	47.980	198	101	2	52	248	8	204	4.19e-60	189
Msa0277850	MtrunA17_Chr3g0103011	41.290	155	90	1	92	246	16	169	6.20e-34	126
Msa0277850	MtrunA17_Chr5g0444791	40.252	159	83	4	92	246	10	160	6.06e-29	109
Msa0277850	MtrunA17_Chr3g0103001	67.442	43	14	0	48	90	35	77	4.25e-15	68.9

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0277850	AT4G26485.1	48.529	204	101	3	46	246	4	206	6.85e-59	186
Msa0277850	AT5G56075.1	47.196	214	94	3	39	249	56	253	1.77e-56	181
Msa0277850	AT1G55790.1	43.662	213	119	1	36	248	7	218	1.22e-54	181
Msa0277850	AT5G25030.1	43.367	196	97	2	52	247	11	192	4.75e-48	158
Msa0277850	AT1G55803.1	49.593	123	62	0	36	158	5	127	1.50e-35	124
Msa0277850	AT5G56060.1	37.333	150	91	2	88	236	2	149	1.41e-30	112
Msa0277850	AT2G11045.1	33.121	157	69	4	92	247	14	135	1.76e-16	74.3
Msa0277850	AT1G55800.1	39.773	88	52	1	161	248	10	96	5.10e-14	70.1

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Find 71 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTCTCTTTGTTGGAGA+GGG	0.521483	2_3:-10678048	Msa0277850:CDS
AAACACAAAATTCTCTTTGT+TGG	0.296100	2_3:-10678054	Msa0277850:CDS
AAAGAAGTGGGACTAGAAGT+TGG	0.519198	2_3:+10675796	None:intergenic
AAAGGAAACATTACTGAATC+TGG	0.348551	2_3:+10677670	None:intergenic
AAAGGCACTAGTAAAGAAAG+AGG	0.540011	2_3:-10676065	Msa0277850:CDS
AAAGTTGTTGAAGGGGTTTA+TGG	0.239937	2_3:-10676095	Msa0277850:CDS
AAATTATTGATTGGTCATTA+GGG	0.285780	2_3:-10678275	Msa0277850:five_prime_UTR
AAGATAGGCTATAATTTGTT+AGG	0.280357	2_3:+10675821	None:intergenic
AAGGCACTAGTAAAGAAAGA+GGG	0.660972	2_3:-10676064	Msa0277850:CDS
AAGGGGTTTGTAATGAATGA+TGG	0.597722	2_3:-10675632	Msa0277850:three_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAATTCTCTTTGTTGGAGA+GGG	-	chr2_3:10675844-10675863	Msa0277850:CDS	30.0%
TAATGGAATGAGTAATGCAA+GGG	-	chr2_3:10676054-10676073	Msa0277850:CDS	30.0%
AAAGGAAACATTACTGAATC+TGG	+	chr2_3:10676225-10676244	None:intergenic	30.0%
ATTGACGTCTCAATTTAACT+TGG	+	chr2_3:10676520-10676539	None:intergenic	30.0%
AAATGTATATGACCACAACT+TGG	+	chr2_3:10676899-10676918	None:intergenic	30.0%
AGTATATTTGACCTTAATCG+TGG	-	chr2_3:10676937-10676956	Msa0277850:intron	30.0%
TCTGTCTATGTTTAACATCA+AGG	+	chr2_3:10676986-10677005	None:intergenic	30.0%
GTAGTTGAATGACTCAAAAA+TGG	+	chr2_3:10677091-10677110	None:intergenic	30.0%
GCAATAGAAATTAAGTAGGT+AGG	-	chr2_3:10677180-10677199	Msa0277850:intron	30.0%
GTGCTTCCTAATAGAATTTA+AGG	-	chr2_3:10677237-10677256	Msa0277850:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_3	gene	10675498	10678416	10675498	ID=Msa0277850;Name=Msa0277850
chr2_3	mRNA	10675498	10678416	10675498	ID=Msa0277850-mRNA-1;Parent=Msa0277850;Name=Msa0277850-mRNA-1;_AED=0.27;_eAED=0.27;_QI=175\|1\|1\|1\|1\|1\|3\|331\|256
chr2_3	exon	10675498	10676126	10675498	ID=Msa0277850-mRNA-1:exon:4722;Parent=Msa0277850-mRNA-1
chr2_3	exon	10677684	10677882	10677684	ID=Msa0277850-mRNA-1:exon:4721;Parent=Msa0277850-mRNA-1
chr2_3	exon	10677968	10678416	10677968	ID=Msa0277850-mRNA-1:exon:4720;Parent=Msa0277850-mRNA-1
chr2_3	five_prime_UTR	10678242	10678416	10678242	ID=Msa0277850-mRNA-1:five_prime_utr;Parent=Msa0277850-mRNA-1
chr2_3	CDS	10677968	10678241	10677968	ID=Msa0277850-mRNA-1:cds;Parent=Msa0277850-mRNA-1
chr2_3	CDS	10677684	10677882	10677684	ID=Msa0277850-mRNA-1:cds;Parent=Msa0277850-mRNA-1
chr2_3	CDS	10675829	10676126	10675829	ID=Msa0277850-mRNA-1:cds;Parent=Msa0277850-mRNA-1
chr2_3	three_prime_UTR	10675498	10675828	10675498	ID=Msa0277850-mRNA-1:three_prime_utr;Parent=Msa0277850-mRNA-1

Gene Sequence

>Msa0277850

ATGATTGAATATCAATTGATCAAAATGAGTCCAACACAAAAACAACATGATCATCCTCAACTTAACACTGTCACTGACACTGATGATGAAGAACAAGAATCAGAGTCAGAAAAAGAAGAAGAATCATCATCGAACACAGCAAAATGGAAAAAACACTACTCATCAAAACACAAAATTCTCTTTGTTGGAGAGGGTGATTTTTCTTTCTCTCTTTGTTTAGCTAGGGCTTTTGGTTCTGCTCATAACCTTATAGCCACTTCCCTTGATTCTCAGGAGAAAATTGAAAAGAAGTATAGTAATGGAATGAGTAATGCAAGGGAACTTGAAGAAAGAGGTTGTATTGTTCTTTATGATGTTGATGTTAAGGTGATGAGTCAGCATTTCTTTCTCAAGACTCAGAGGTTTGATCGTGTTGTTTATAATTTTCCTCATGTTGGATTCCTTTACCCTGAGAATAGTTACTGCCAGATTCAGTTGAACAAAAAGTTGTTGAAGGGGTTTATGGCAAATGCAAAGGCACTAGTAAAGAAAGAGGGAGGGGAGATTCATGTAACTCACAAAGAGGGTGATCCATACAACAAATGGGATCTTGTAAGAAAAGCAGAAAAAAGAGGACTGTTCTTACACCAAGCTGTTCCATTCTTCAAAGATGACTATCCTGGTTATGACAACAAGAGAGCTCATGGAAAGTTATCAGATTTATCTTTCCCTGTTGGGGAAGCCAGTACTTACAAGTTCAAGCTCCAAACACCTCTCCCTAACAAATTATAG

Protein sequence

>Msa0277850

MIEYQLIKMSPTQKQHDHPQLNTVTDTDDEEQESESEKEEESSSNTAKWKKHYSSKHKILFVGEGDFSFSLCLARAFGSAHNLIATSLDSQEKIEKKYSNGMSNARELEERGCIVLYDVDVKVMSQHFFLKTQRFDRVVYNFPHVGFLYPENSYCQIQLNKKLLKGFMANAKALVKKEGGEIHVTHKEGDPYNKWDLVRKAEKRGLFLHQAVPFFKDDYPGYDNKRAHGKLSDLSFPVGEASTYKFKLQTPLPNKL*