AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0204300

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0204300 MtrunA17_Chr2g0302421 96.000 125 5 0 1 125 378 502 3.58e-83 251
Msa0204300 MtrunA17_Chr4g0069631 70.400 125 34 1 1 125 376 497 1.82e-64 203
Msa0204300 MtrunA17_Chr7g0271351 60.000 125 47 1 1 125 383 504 7.57e-53 173
Msa0204300 MtrunA17_Chr2g0288851 54.400 125 54 1 1 125 389 510 2.65e-50 166
Msa0204300 MtrunA17_Chr1g0193211 53.600 125 55 1 1 125 387 508 3.40e-49 163
Msa0204300 MtrunA17_Chr4g0075181 46.400 125 63 1 1 125 399 519 1.39e-42 146
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0204300 AT1G11910.1 51.200 125 58 1 1 125 385 506 1.87e-45 154
Msa0204300 AT1G11910.2 51.200 125 58 1 1 125 396 517 2.59e-45 154
Msa0204300 AT1G62290.1 48.387 124 61 1 1 124 392 512 1.31e-44 152
Msa0204300 AT1G62290.2 48.387 124 61 1 1 124 392 512 1.31e-44 152
Msa0204300 AT4G04460.1 42.400 125 69 1 1 125 387 508 4.81e-37 131
Msa0204300 AT4G04460.2 42.400 125 69 1 1 125 383 504 4.96e-37 131
Msa0204300 AT1G62290.5 46.939 98 49 1 1 98 392 486 3.53e-31 115
Msa0204300 AT1G62290.6 44.615 65 36 0 1 65 392 456 1.73e-19 83.2
Msa0204300 AT1G62290.4 44.615 65 36 0 1 65 392 456 1.73e-19 83.2
Msa0204300 AT4G22050.1 37.079 89 42 3 40 125 276 353 3.92e-11 59.3

Find 107 sgRNAs with CRISPR-Local

Find 241 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTAAAACGAACTGAAAATT+TGG 0.095495 2_1:-29249362 None:intergenic
AGCGTATTTCATCTAAAATT+TGG 0.195496 2_1:-29249576 None:intergenic
AACATCACTGCAAGTAAAAT+TGG 0.238677 2_1:-29250307 None:intergenic
ATGTCAGCACATAAGATTTC+AGG 0.241847 2_1:-29250175 None:intergenic
AACGATTGTCCAACCGGATT+TGG 0.243320 2_1:-29250892 None:intergenic
CAAAAGAGACTCTCTCTTAA+TGG 0.252205 2_1:-29250369 None:intergenic
ATATGGGAATCATTGATTTC+AGG 0.264136 2_1:+29249904 Msa0204300:five_prime_UTR
ATTAGACTTTGTTCAGATAA+TGG 0.288447 2_1:+29250196 Msa0204300:five_prime_UTR
CTTTAAATACAAACCTGTTC+TGG 0.292662 2_1:-29250988 None:intergenic
CCAAACATTACATTCACATT+TGG 0.310989 2_1:+29250943 Msa0204300:CDS
CTAACTAAATTATACAAAAC+TGG 0.330981 2_1:-29249547 None:intergenic
ATAACTACGGAAATTCGATA+TGG 0.362943 2_1:+29249887 Msa0204300:five_prime_UTR
ATATCGAATTTCCGTAGTTA+TGG 0.381442 2_1:-29249885 None:intergenic
TTATACAAAACTGGTTTGTA+AGG 0.391173 2_1:-29249538 None:intergenic
GTTTCCTCTCTCTCCAGAAC+AGG 0.392495 2_1:+29250975 Msa0204300:CDS
TGATTTCGTAGGTTTGAGTT+AGG 0.402916 2_1:+29249333 Msa0204300:five_prime_UTR
CAGATTCTGCAAATCCTATA+CGG 0.404994 2_1:-29251513 None:intergenic
TCTTGATGTGCCTTCCCCAC+AGG 0.408161 2_1:+29251298 Msa0204300:CDS
TTTATAAACGATTGTCCAAC+CGG 0.409626 2_1:-29250898 None:intergenic
CTGTGCTGCAATAGTGGACT+CGG 0.415057 2_1:+29249687 Msa0204300:five_prime_UTR
TATGGGAATCATTGATTTCA+GGG 0.417684 2_1:+29249905 Msa0204300:five_prime_UTR
CTAAAACGAACTGAAAATTT+GGG 0.423041 2_1:-29249361 None:intergenic
CCAAATGTGAATGTAATGTT+TGG 0.425255 2_1:-29250943 None:intergenic
TTTGCAATATGGTTGTCCTT+TGG 0.425536 2_1:+29250397 Msa0204300:exon
GTGTGAGAGGCTTCCAAATC+CGG 0.429661 2_1:+29250879 Msa0204300:CDS
TCGGGGACTACTTTAATTGC+CGG 0.429816 2_1:+29249706 Msa0204300:five_prime_UTR
AGCTAAGGAATGTGTCATAA+AGG 0.430691 2_1:-29250264 None:intergenic
TGCAAATCCTATACGGAGAT+TGG 0.430860 2_1:-29251506 None:intergenic
CTCATATATAAGATGAGATA+TGG 0.431700 2_1:+29249480 Msa0204300:five_prime_UTR
GCACGGACACTCCTCGAATT+AGG 0.437199 2_1:-29250088 None:intergenic
CGAAATCAGCTCGTAAGGTT+GGG 0.458034 2_1:-29249318 None:intergenic
ATTTCATCTAAAATTTGGAT+AGG 0.459956 2_1:-29249571 None:intergenic
TGCAAATCCTATGCGGAGAT+TGG 0.460464 2_1:-29253126 None:intergenic
CTTCCCCACAGGGTCCCCTC+TGG 0.461021 2_1:+29251309 Msa0204300:CDS
CAGTGGTGCATAATGAAAGT+TGG 0.466959 2_1:+29250337 Msa0204300:five_prime_UTR
GGGTGGCTGTGCTGCAATAG+TGG 0.468506 2_1:+29249681 Msa0204300:five_prime_UTR
ACAAGAACTAGTAAGAAGAA+AGG 0.471602 2_1:-29249785 None:intergenic
CAAGAACTAGTAAGAAGAAA+GGG 0.476891 2_1:-29249784 None:intergenic
ATATGTATGTGCAGGGTTGT+TGG 0.477358 2_1:+29251442 Msa0204300:intron
CTTGATGTGCCTTCCCCACA+GGG 0.478328 2_1:+29251299 Msa0204300:CDS
TTCTTTCAAAGCTGTGTGAG+AGG 0.492077 2_1:+29250866 Msa0204300:intron
GTCTAAAATGACTTTATAAG+TGG 0.492674 2_1:+29249502 Msa0204300:five_prime_UTR
ACGAAATCAGCTCGTAAGGT+TGG 0.492686 2_1:-29249319 None:intergenic
CTTGAAAATGAGTTCATAAG+TGG 0.492744 2_1:+29249288 Msa0204300:five_prime_UTR
TTTCATCTAAAATTTGGATA+GGG 0.494290 2_1:-29249570 None:intergenic
CAGAGGGGACCCTGTGGGGA+AGG 0.496212 2_1:-29251308 None:intergenic
TCTCAAATTATTAGCGGTGT+CGG 0.499451 2_1:-29250014 None:intergenic
ATACGTATGTGCAGGGTTGT+TGG 0.500999 2_1:+29253062 Msa0204300:intron
GTGTGTATCCAAAATACGTT+AGG 0.501923 2_1:+29249383 Msa0204300:five_prime_UTR
AACCATGCCATTGGAACAGA+AGG 0.503270 2_1:+29249832 Msa0204300:five_prime_UTR
AGTGGTGCATAATGAAAGTT+GGG 0.513363 2_1:+29250338 Msa0204300:five_prime_UTR
TAACTACGGAAATTCGATAT+GGG 0.515650 2_1:+29249888 Msa0204300:five_prime_UTR
AGGAATCAAGAATAAGAAGA+TGG 0.516613 2_1:-29250244 None:intergenic
AAATACGCTGGTGCCGTTGT+TGG 0.516875 2_1:+29249590 Msa0204300:five_prime_UTR
TCCTCGAATTAGGCGTGTCC+CGG 0.517240 2_1:-29250078 None:intergenic
ACTCAAATTAACCATGCCAT+TGG 0.518999 2_1:+29249823 Msa0204300:five_prime_UTR
TGTTAAGTTAGATGTAGCTA+AGG 0.527869 2_1:-29250279 None:intergenic
AACAAATCCTTCTGTTCCAA+TGG 0.528562 2_1:-29249839 None:intergenic
ACTCGAGGAAACATGATAGC+GGG 0.530739 2_1:-29249409 None:intergenic
ACCTACGAAATCAGCTCGTA+AGG 0.531541 2_1:-29249323 None:intergenic
GAGATGTTATACAGTTGGAC+CGG 0.532060 2_1:-29249725 None:intergenic
ATCCTTCTGTTCCAATGGCA+TGG 0.535909 2_1:-29249834 None:intergenic
GATTAAGCAAAGCAATGTAA+AGG 0.536096 2_1:+29250431 Msa0204300:CDS
TCGTATCCGTGCTTCGTAGG+TGG 0.545277 2_1:-29249974 None:intergenic
TGTTCTCCTGTGTGCTATAG+CGG 0.548629 2_1:+29251266 Msa0204300:CDS
AATTAGGCGTGTCCCGGTGT+CGG 0.549270 2_1:-29250072 None:intergenic
GACTACGCCAATCTCCGTAT+AGG 0.550968 2_1:+29251499 Msa0204300:CDS
AAATTTGAATTAATGAAGCA+CGG 0.551198 2_1:-29250105 None:intergenic
GACTATCCACCTACGAAGCA+CGG 0.558281 2_1:+29249968 Msa0204300:five_prime_UTR
TGTGCTGCAATAGTGGACTC+GGG 0.559334 2_1:+29249688 Msa0204300:five_prime_UTR
TGTCAAGTCTAGAGGGTGAT+GGG 0.566768 2_1:+29249443 Msa0204300:five_prime_UTR
AAATGTTAAGAGTATCGCAT+TGG 0.568221 2_1:+29249254 Msa0204300:five_prime_UTR
ATGTCAAGTCTAGAGGGTGA+TGG 0.572248 2_1:+29249442 Msa0204300:five_prime_UTR
ACCGGGACACGCCTAATTCG+AGG 0.573058 2_1:+29250077 Msa0204300:five_prime_UTR
ATGTGTCAGTGTCCGACACC+GGG 0.574461 2_1:+29250060 Msa0204300:five_prime_UTR
CAAACCTGTTCTGGAGAGAG+AGG 0.576097 2_1:-29250979 None:intergenic
ACCTTACGAGCTGATTTCGT+AGG 0.580385 2_1:+29249322 Msa0204300:five_prime_UTR
GCTTCAGATGTCAAGTCTAG+AGG 0.592660 2_1:+29249435 Msa0204300:five_prime_UTR
CTTCAGATGTCAAGTCTAGA+GGG 0.597080 2_1:+29249436 Msa0204300:five_prime_UTR
TGTTCGTATCCGTGCTTCGT+AGG 0.599140 2_1:-29249977 None:intergenic
TCTAAAATGACTTTATAAGT+GGG 0.600335 2_1:+29249503 Msa0204300:five_prime_UTR
CAAAACTGGTTTGTAAGGTG+AGG 0.601839 2_1:-29249533 None:intergenic
TATGTGTCAGTGTCCGACAC+CGG 0.607871 2_1:+29250059 Msa0204300:five_prime_UTR
TTGAAAATGAGTTCATAAGT+GGG 0.613853 2_1:+29249289 Msa0204300:five_prime_UTR
GTGCATAATGAAAGTTGGGA+TGG 0.614926 2_1:+29250342 Msa0204300:five_prime_UTR
ATGGGAATCATTGATTTCAG+GGG 0.615119 2_1:+29249906 Msa0204300:five_prime_UTR
CTCGAGGAAACATGATAGCG+GGG 0.622694 2_1:-29249408 None:intergenic
GAGAGGCTTCCAAATCCGGT+TGG 0.622716 2_1:+29250883 Msa0204300:CDS
GACTACGCCAATCTCCGCAT+AGG 0.629607 2_1:+29253119 Msa0204300:three_prime_UTR
ATTTACACAAGTTTATGTTG+TGG 0.630103 2_1:-29249639 None:intergenic
AGTATTAAAATATGTCGATG+AGG 0.637042 2_1:+29250458 Msa0204300:CDS
AAAATGACTTTATAAGTGGG+AGG 0.638657 2_1:+29249506 Msa0204300:five_prime_UTR
ATTACCAGAGGGGACCCTGT+GGG 0.641259 2_1:-29251313 None:intergenic
GTGCTGCAATAGTGGACTCG+GGG 0.644938 2_1:+29249689 Msa0204300:five_prime_UTR
TCGAGGAAACATGATAGCGG+GGG 0.646532 2_1:-29249407 None:intergenic
GACATCTGAAGCATGACTCG+AGG 0.649006 2_1:-29249424 None:intergenic
TTTACAGGTTTATGTCAGGG+TGG 0.650609 2_1:+29249664 Msa0204300:five_prime_UTR
TTACCAGAGGGGACCCTGTG+GGG 0.651193 2_1:-29251312 None:intergenic
ACGAAGCACGGATACGAACA+CGG 0.658382 2_1:+29249980 Msa0204300:five_prime_UTR
CGAGGAAACATGATAGCGGG+GGG 0.677326 2_1:-29249406 None:intergenic
CAGTGATGTTATTGAAACAG+TGG 0.692257 2_1:+29250320 Msa0204300:five_prime_UTR
GACTCGAGGAAACATGATAG+CGG 0.692571 2_1:-29249410 None:intergenic
CAGATTCTGCAAATCCTATG+CGG 0.703090 2_1:-29253133 None:intergenic
TATTACCAGAGGGGACCCTG+TGG 0.703309 2_1:-29251314 None:intergenic
ATGAACATGAGTGCCAACAA+CGG 0.712828 2_1:-29249603 None:intergenic
ACAAAACCGCTATAGCACAC+AGG 0.715098 2_1:-29251272 None:intergenic
ATAAGTATGTATTACCAGAG+GGG 0.771682 2_1:-29251323 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTGTTTTATTTAAATTACAA+AGG + chr2_1:29250517-29250536 Msa0204300:intron 15.0%
!!! ATAACTTTATTTATCCAATT+AGG + chr2_1:29250811-29250830 Msa0204300:intron 15.0%
!!! TCTAAAATGACTTTATAAGT+GGG + chr2_1:29249503-29249522 Msa0204300:five_prime_UTR 20.0%
!! CTAACTAAATTATACAAAAC+TGG - chr2_1:29249550-29249569 None:intergenic 20.0%
!!! TTTCATCTAAAATTTGGATA+GGG - chr2_1:29249573-29249592 None:intergenic 20.0%
!!! ATTTCATCTAAAATTTGGAT+AGG - chr2_1:29249574-29249593 None:intergenic 20.0%
!!! AACTTGTGTAAATATTTTAC+AGG + chr2_1:29249649-29249668 Msa0204300:five_prime_UTR 20.0%
!!! TGATTTTCTCAAATTATTAG+CGG - chr2_1:29250023-29250042 None:intergenic 20.0%
!! AAATTTGAATTAATGAAGCA+CGG - chr2_1:29250108-29250127 None:intergenic 20.0%
!!! TTTAGAGTAAACATAGATAT+AGG + chr2_1:29250549-29250568 Msa0204300:intron 20.0%
!! ATTTAGAGACTATATATTCA+AGG - chr2_1:29250576-29250595 None:intergenic 20.0%
!!! TAACTATTAGAAGACATTTT+CGG - chr2_1:29250622-29250641 None:intergenic 20.0%
!! TATTCTTATATTCGTTAGTT+TGG + chr2_1:29250666-29250685 Msa0204300:intron 20.0%
!! TCCTTATTAAAAAACATAGA+TGG + chr2_1:29251717-29251736 Msa0204300:intron 20.0%
!!! TCCATCTATGTTTTTTAATA+AGG - chr2_1:29251721-29251740 None:intergenic 20.0%
!!! TTTTATTCCTACTGTTATTA+TGG - chr2_1:29252016-29252035 None:intergenic 20.0%
!!! TAGTTTTTTTTTTTGGCTTT+TGG - chr2_1:29252207-29252226 None:intergenic 20.0%
!!! GTCTTAGTAGTTTTTTTTTT+TGG - chr2_1:29252214-29252233 None:intergenic 20.0%
!! GAAAATTAGAACTAAAAGAA+CGG - chr2_1:29252261-29252280 None:intergenic 20.0%
!!! TTACATTAACTTGCTAATTT+AGG - chr2_1:29252790-29252809 None:intergenic 20.0%
! TTGAAAATGAGTTCATAAGT+GGG + chr2_1:29249289-29249308 Msa0204300:five_prime_UTR 25.0%
! CTAAAACGAACTGAAAATTT+GGG - chr2_1:29249364-29249383 None:intergenic 25.0%
! ACTAAAACGAACTGAAAATT+TGG - chr2_1:29249365-29249384 None:intergenic 25.0%
! CTCATATATAAGATGAGATA+TGG + chr2_1:29249480-29249499 Msa0204300:five_prime_UTR 25.0%
!! GTCTAAAATGACTTTATAAG+TGG + chr2_1:29249502-29249521 Msa0204300:five_prime_UTR 25.0%
!!! TTATACAAAACTGGTTTGTA+AGG - chr2_1:29249541-29249560 None:intergenic 25.0%
!! AGCGTATTTCATCTAAAATT+TGG - chr2_1:29249579-29249598 None:intergenic 25.0%
!! AAATTTTAGATGAAATACGC+TGG + chr2_1:29249578-29249597 Msa0204300:five_prime_UTR 25.0%
! ATTTACACAAGTTTATGTTG+TGG - chr2_1:29249642-29249661 None:intergenic 25.0%
!! ATATTTTACAGGTTTATGTC+AGG + chr2_1:29249660-29249679 Msa0204300:five_prime_UTR 25.0%
!! TATTTTACAGGTTTATGTCA+GGG + chr2_1:29249661-29249680 Msa0204300:five_prime_UTR 25.0%
!!! TTTTAGAGATGTTATACAGT+TGG - chr2_1:29249733-29249752 None:intergenic 25.0%
! AAAAACATTGTCCATAACTA+CGG + chr2_1:29249874-29249893 Msa0204300:five_prime_UTR 25.0%
! ATTAGACTTTGTTCAGATAA+TGG + chr2_1:29250196-29250215 Msa0204300:five_prime_UTR 25.0%
!! CTTTTGTAGTTTTTGCAATA+TGG + chr2_1:29250386-29250405 Msa0204300:exon 25.0%
! AGTATTAAAATATGTCGATG+AGG + chr2_1:29250458-29250477 Msa0204300:CDS 25.0%
!! AACTATTAGAAGACATTTTC+GGG - chr2_1:29250621-29250640 None:intergenic 25.0%
!! GTTAGTTTAATCATCAAATC+AGG + chr2_1:29250638-29250657 Msa0204300:intron 25.0%
! AATATGTAGATAATGTCTAC+TGG + chr2_1:29251609-29251628 Msa0204300:intron 25.0%
! TGGAAATAAATCAATTGAGT+TGG + chr2_1:29251737-29251756 Msa0204300:intron 25.0%
! GGAAATAAATCAATTGAGTT+GGG + chr2_1:29251738-29251757 Msa0204300:intron 25.0%
! GTGCAGAAAATGAAATATAT+AGG + chr2_1:29252059-29252078 Msa0204300:intron 25.0%
! AAAATGAAATATATAGGAGC+TGG + chr2_1:29252065-29252084 Msa0204300:intron 25.0%
!!! TTAATCTCTAAAATTTGCGA+AGG - chr2_1:29252117-29252136 None:intergenic 25.0%
! GTTACTCTAATCATAATATC+CGG + chr2_1:29252175-29252194 Msa0204300:intron 25.0%
! TCTGATTTATGTTACATCAT+TGG - chr2_1:29252898-29252917 None:intergenic 25.0%
!! AAATGTTAAGAGTATCGCAT+TGG + chr2_1:29249254-29249273 Msa0204300:five_prime_UTR 30.0%
CTTGAAAATGAGTTCATAAG+TGG + chr2_1:29249288-29249307 Msa0204300:five_prime_UTR 30.0%
AAAATGACTTTATAAGTGGG+AGG + chr2_1:29249506-29249525 Msa0204300:five_prime_UTR 30.0%
CAAGAACTAGTAAGAAGAAA+GGG - chr2_1:29249787-29249806 None:intergenic 30.0%
ACAAGAACTAGTAAGAAGAA+AGG - chr2_1:29249788-29249807 None:intergenic 30.0%
!!! TCTTACTAGTTCTTGTTTTC+AGG + chr2_1:29249792-29249811 Msa0204300:five_prime_UTR 30.0%
!!! CTTACTAGTTCTTGTTTTCA+GGG + chr2_1:29249793-29249812 Msa0204300:five_prime_UTR 30.0%
ATATCGAATTTCCGTAGTTA+TGG - chr2_1:29249888-29249907 None:intergenic 30.0%
ATAACTACGGAAATTCGATA+TGG + chr2_1:29249887-29249906 Msa0204300:five_prime_UTR 30.0%
TAACTACGGAAATTCGATAT+GGG + chr2_1:29249888-29249907 Msa0204300:five_prime_UTR 30.0%
!! ATATGGGAATCATTGATTTC+AGG + chr2_1:29249904-29249923 Msa0204300:five_prime_UTR 30.0%
!! TATGGGAATCATTGATTTCA+GGG + chr2_1:29249905-29249924 Msa0204300:five_prime_UTR 30.0%
AGGAATCAAGAATAAGAAGA+TGG - chr2_1:29250247-29250266 None:intergenic 30.0%
TGTTAAGTTAGATGTAGCTA+AGG - chr2_1:29250282-29250301 None:intergenic 30.0%
AACATCACTGCAAGTAAAAT+TGG - chr2_1:29250310-29250329 None:intergenic 30.0%
! TAATCTGATTTTGCATCCAA+AGG - chr2_1:29250416-29250435 None:intergenic 30.0%
GATTAAGCAAAGCAATGTAA+AGG + chr2_1:29250431-29250450 Msa0204300:CDS 30.0%
TTTATAAACGATTGTCCAAC+CGG - chr2_1:29250901-29250920 None:intergenic 30.0%
CCAAATGTGAATGTAATGTT+TGG - chr2_1:29250946-29250965 None:intergenic 30.0%
CCAAACATTACATTCACATT+TGG + chr2_1:29250943-29250962 Msa0204300:CDS 30.0%
CTTTAAATACAAACCTGTTC+TGG - chr2_1:29250991-29251010 None:intergenic 30.0%
ATTCACGTTCATCTAACAAA+TGG - chr2_1:29251037-29251056 None:intergenic 30.0%
AATGTGAAATCAATGAATGC+AGG - chr2_1:29251089-29251108 None:intergenic 30.0%
AACATTTACAGGAAGGAAAA+TGG - chr2_1:29251120-29251139 None:intergenic 30.0%
TAAAGAAACCAGATATGCTA+CGG - chr2_1:29251179-29251198 None:intergenic 30.0%
ATAAGTATGTATTACCAGAG+GGG - chr2_1:29251326-29251345 None:intergenic 30.0%
GATAAGTATGTATTACCAGA+GGG - chr2_1:29251327-29251346 None:intergenic 30.0%
TGATAAGTATGTATTACCAG+AGG - chr2_1:29251328-29251347 None:intergenic 30.0%
TGCACATACATATATCACTA+CGG - chr2_1:29251435-29251454 None:intergenic 30.0%
TAGTGATATATGTATGTGCA+GGG + chr2_1:29251435-29251454 Msa0204300:intron 30.0%
!! TAGATAATGTCTACTGGTTT+AGG + chr2_1:29251615-29251634 Msa0204300:intron 30.0%
AATACTCACACTCACTATTA+TGG - chr2_1:29251665-29251684 None:intergenic 30.0%
ACAAGAAGGAATAATGGTAA+TGG - chr2_1:29251782-29251801 None:intergenic 30.0%
TCTCAAACAAGAAGGAATAA+TGG - chr2_1:29251788-29251807 None:intergenic 30.0%
ATACAGATTCTCAAACAAGA+AGG - chr2_1:29251796-29251815 None:intergenic 30.0%
GAATCTGTATACAAATTGAG+AGG + chr2_1:29251806-29251825 Msa0204300:intron 30.0%
AGGGTATATGAGTACAAATT+GGG + chr2_1:29251877-29251896 Msa0204300:intron 30.0%
TAAATAGTGTAGAGACTTAG+AGG + chr2_1:29251946-29251965 Msa0204300:intron 30.0%
TAGAGACTTAGAGGTAATAA+AGG + chr2_1:29251955-29251974 Msa0204300:intron 30.0%
AACACAACCATAATAACAGT+AGG + chr2_1:29252006-29252025 Msa0204300:intron 30.0%
TTTATTCCTGGCATCTAAAT+TGG - chr2_1:29252288-29252307 None:intergenic 30.0%
! TTCACTTGGTTCAAAAGTAT+CGG - chr2_1:29252514-29252533 None:intergenic 30.0%
TTATTGGATATCAGTTCACT+TGG - chr2_1:29252528-29252547 None:intergenic 30.0%
CTGTTTGATATACCTCTTAT+TGG - chr2_1:29252544-29252563 None:intergenic 30.0%
! CAATAAGAGGTATATCAAAC+AGG + chr2_1:29252542-29252561 Msa0204300:intron 30.0%
CTCAAGAAGAAGATAATCTT+AGG - chr2_1:29252718-29252737 None:intergenic 30.0%
! CTTATTGAAACTCTAGTTCA+TGG - chr2_1:29252931-29252950 None:intergenic 30.0%
! AAAAACAAGAGCATGAAACA+TGG - chr2_1:29251386-29251405 None:intergenic 30.0%
! TGATTTCGTAGGTTTGAGTT+AGG + chr2_1:29249333-29249352 Msa0204300:five_prime_UTR 35.0%
GTGTGTATCCAAAATACGTT+AGG + chr2_1:29249383-29249402 Msa0204300:five_prime_UTR 35.0%
ACTCAAATTAACCATGCCAT+TGG + chr2_1:29249823-29249842 Msa0204300:five_prime_UTR 35.0%
AACAAATCCTTCTGTTCCAA+TGG - chr2_1:29249842-29249861 None:intergenic 35.0%
!! ATGGGAATCATTGATTTCAG+GGG + chr2_1:29249906-29249925 Msa0204300:five_prime_UTR 35.0%
TCTCAAATTATTAGCGGTGT+CGG - chr2_1:29250017-29250036 None:intergenic 35.0%
ATGTCAGCACATAAGATTTC+AGG - chr2_1:29250178-29250197 None:intergenic 35.0%
AGCTAAGGAATGTGTCATAA+AGG - chr2_1:29250267-29250286 None:intergenic 35.0%
CAGTGATGTTATTGAAACAG+TGG + chr2_1:29250320-29250339 Msa0204300:five_prime_UTR 35.0%
AGTGGTGCATAATGAAAGTT+GGG + chr2_1:29250338-29250357 Msa0204300:five_prime_UTR 35.0%
CAAAAGAGACTCTCTCTTAA+TGG - chr2_1:29250372-29250391 None:intergenic 35.0%
TTTGCAATATGGTTGTCCTT+TGG + chr2_1:29250397-29250416 Msa0204300:exon 35.0%
AAAGCTTCTGACTTCCTAAT+TGG - chr2_1:29250828-29250847 None:intergenic 35.0%
ACACTCTAACATTTACAGGA+AGG - chr2_1:29251127-29251146 None:intergenic 35.0%
CAACACACTCTAACATTTAC+AGG - chr2_1:29251131-29251150 None:intergenic 35.0%
GAACATACTGAGAAGGAAAA+AGG - chr2_1:29251220-29251239 None:intergenic 35.0%
! ACTCTAAGAACATACTGAGA+AGG - chr2_1:29251227-29251246 None:intergenic 35.0%
GTAGTGATATATGTATGTGC+AGG + chr2_1:29251434-29251453 Msa0204300:intron 35.0%
! TGTTGGAGATATTTTCTTGC+AGG + chr2_1:29251459-29251478 Msa0204300:CDS 35.0%
CAGATTCTGCAAATCCTATA+CGG - chr2_1:29251516-29251535 None:intergenic 35.0%
! GGTTTAGGTAAGAGTATGTA+AGG + chr2_1:29251630-29251649 Msa0204300:intron 35.0%
!! AGGAATAATGGTAATGGTGA+AGG - chr2_1:29251776-29251795 None:intergenic 35.0%
! GAGGGTATATGAGTACAAAT+TGG + chr2_1:29251876-29251895 Msa0204300:intron 35.0%
TCTAAACCAATTTAGATGCC+AGG + chr2_1:29252279-29252298 Msa0204300:intron 35.0%
!!! AGTCGTAGGTTTTTTATTCC+TGG - chr2_1:29252300-29252319 None:intergenic 35.0%
! GGATCAAGTTGTAAAAGAAG+TGG + chr2_1:29252373-29252392 Msa0204300:intron 35.0%
GTGAACTGATATCCAATAAG+AGG + chr2_1:29252529-29252548 Msa0204300:intron 35.0%
GCATCAAGAAACGAATTAGT+TGG - chr2_1:29252982-29253001 None:intergenic 35.0%
TAGTGATATACGTATGTGCA+GGG + chr2_1:29253055-29253074 Msa0204300:intron 35.0%
! TGTTGGAGATATTTTCTTGC+AGG + chr2_1:29251459-29251478 Msa0204300:CDS 35.0%
CTTCAGATGTCAAGTCTAGA+GGG + chr2_1:29249436-29249455 Msa0204300:five_prime_UTR 40.0%
!! CAAAACTGGTTTGTAAGGTG+AGG - chr2_1:29249536-29249555 None:intergenic 40.0%
ATGAACATGAGTGCCAACAA+CGG - chr2_1:29249606-29249625 None:intergenic 40.0%
TTTACAGGTTTATGTCAGGG+TGG + chr2_1:29249664-29249683 Msa0204300:five_prime_UTR 40.0%
GAGATGTTATACAGTTGGAC+CGG - chr2_1:29249728-29249747 None:intergenic 40.0%
CAGTGGTGCATAATGAAAGT+TGG + chr2_1:29250337-29250356 Msa0204300:five_prime_UTR 40.0%
GTGCATAATGAAAGTTGGGA+TGG + chr2_1:29250342-29250361 Msa0204300:five_prime_UTR 40.0%
! ACAACACCCTCACACATTTT+GGG + chr2_1:29250760-29250779 Msa0204300:intron 40.0%
TTCTTTCAAAGCTGTGTGAG+AGG + chr2_1:29250866-29250885 Msa0204300:intron 40.0%
AGGACTCATGAAGATAAGTG+AGG - chr2_1:29251069-29251088 None:intergenic 40.0%
GAATGTATCCGTAGCATATC+TGG + chr2_1:29251168-29251187 Msa0204300:intron 40.0%
ATATGTATGTGCAGGGTTGT+TGG + chr2_1:29251442-29251461 Msa0204300:intron 40.0%
TGCAAATCCTATACGGAGAT+TGG - chr2_1:29251509-29251528 None:intergenic 40.0%
!! TCAATTGAGTTGGGAATCTG+TGG + chr2_1:29251747-29251766 Msa0204300:intron 40.0%
TGAGAGGCAGAAAATGTAGT+AGG + chr2_1:29251822-29251841 Msa0204300:intron 40.0%
! ATATGCAAGTTGAGCACAAG+AGG + chr2_1:29251857-29251876 Msa0204300:intron 40.0%
! TATGCAAGTTGAGCACAAGA+GGG + chr2_1:29251858-29251877 Msa0204300:intron 40.0%
!!! TTTTGGCTTTTGGTTCTAGC+CGG - chr2_1:29252197-29252216 None:intergenic 40.0%
GAGTGATCTGTCAATTGAAG+TGG + chr2_1:29252320-29252339 Msa0204300:intron 40.0%
GTCAATTGAAGTGGACACTA+CGG + chr2_1:29252329-29252348 Msa0204300:intron 40.0%
! AAGCATCGCATTCAGGAATA+AGG + chr2_1:29252429-29252448 Msa0204300:intron 40.0%
TTCAGGAATAAGGATTGCCA+AGG + chr2_1:29252439-29252458 Msa0204300:intron 40.0%
TTGCCAAGGAGATGTAACAA+AGG + chr2_1:29252453-29252472 Msa0204300:intron 40.0%
ACACCTTTGTTACATCTCCT+TGG - chr2_1:29252459-29252478 None:intergenic 40.0%
AAGGAGATGTAACAAAGGTG+TGG + chr2_1:29252458-29252477 Msa0204300:intron 40.0%
ATTTCTACAACAGACAAGCG+AGG - chr2_1:29252628-29252647 None:intergenic 40.0%
TAACGATCGTGGTGCATAAT+TGG - chr2_1:29252667-29252686 None:intergenic 40.0%
AGTAACTTCTCTAACGATCG+TGG - chr2_1:29252678-29252697 None:intergenic 40.0%
CGTTAGAGAAGTTACTGAGA+TGG + chr2_1:29252682-29252701 Msa0204300:intron 40.0%
TGGAGACTCACATCATTCTA+TGG - chr2_1:29252750-29252769 None:intergenic 40.0%
ATAGAATGATGTGAGTCTCC+AGG + chr2_1:29252749-29252768 Msa0204300:intron 40.0%
AGTCTCCAGGTATCAATTGT+AGG + chr2_1:29252762-29252781 Msa0204300:intron 40.0%
GTCTCCAGGTATCAATTGTA+GGG + chr2_1:29252763-29252782 Msa0204300:intron 40.0%
CATTTCTCCGTTGATCGAAT+TGG - chr2_1:29252865-29252884 None:intergenic 40.0%
ACGATGTCCGATTATAGACA+CGG + chr2_1:29252955-29252974 Msa0204300:intron 40.0%
AGTTGGTCCGTGTCTATAAT+CGG - chr2_1:29252965-29252984 None:intergenic 40.0%
GTAGTGATATACGTATGTGC+AGG + chr2_1:29253054-29253073 Msa0204300:intron 40.0%
CAGATTCTGCAAATCCTATG+CGG - chr2_1:29253136-29253155 None:intergenic 40.0%
CGAAATCAGCTCGTAAGGTT+GGG - chr2_1:29249321-29249340 None:intergenic 45.0%
ACGAAATCAGCTCGTAAGGT+TGG - chr2_1:29249322-29249341 None:intergenic 45.0%
! ACCTTACGAGCTGATTTCGT+AGG + chr2_1:29249322-29249341 Msa0204300:five_prime_UTR 45.0%
ACCTACGAAATCAGCTCGTA+AGG - chr2_1:29249326-29249345 None:intergenic 45.0%
ACTCGAGGAAACATGATAGC+GGG - chr2_1:29249412-29249431 None:intergenic 45.0%
GACTCGAGGAAACATGATAG+CGG - chr2_1:29249413-29249432 None:intergenic 45.0%
GCTTCAGATGTCAAGTCTAG+AGG + chr2_1:29249435-29249454 Msa0204300:five_prime_UTR 45.0%
ATGTCAAGTCTAGAGGGTGA+TGG + chr2_1:29249442-29249461 Msa0204300:five_prime_UTR 45.0%
TGTCAAGTCTAGAGGGTGAT+GGG + chr2_1:29249443-29249462 Msa0204300:five_prime_UTR 45.0%
TCGGGGACTACTTTAATTGC+CGG + chr2_1:29249706-29249725 Msa0204300:five_prime_UTR 45.0%
AACCATGCCATTGGAACAGA+AGG + chr2_1:29249832-29249851 Msa0204300:five_prime_UTR 45.0%
ATCCTTCTGTTCCAATGGCA+TGG - chr2_1:29249837-29249856 None:intergenic 45.0%
! GACAACACCCTCACACATTT+TGG + chr2_1:29250759-29250778 Msa0204300:intron 45.0%
CTTCGTCCCAAAATGTGTGA+GGG - chr2_1:29250769-29250788 None:intergenic 45.0%
TCTTCGTCCCAAAATGTGTG+AGG - chr2_1:29250770-29250789 None:intergenic 45.0%
AACGATTGTCCAACCGGATT+TGG - chr2_1:29250895-29250914 None:intergenic 45.0%
TGTTCTCCTGTGTGCTATAG+CGG + chr2_1:29251266-29251285 Msa0204300:CDS 45.0%
ACAAAACCGCTATAGCACAC+AGG - chr2_1:29251275-29251294 None:intergenic 45.0%
ATTAGCATGTGTCGAGATGC+AGG + chr2_1:29251914-29251933 Msa0204300:intron 45.0%
TTGACAGATCACTCAGTCGT+AGG - chr2_1:29252314-29252333 None:intergenic 45.0%
AATTGAAGTGGACACTACGG+TGG + chr2_1:29252332-29252351 Msa0204300:intron 45.0%
ATTGAAGTGGACACTACGGT+GGG + chr2_1:29252333-29252352 Msa0204300:intron 45.0%
!! GACTCTAAAGCATCGCATTC+AGG + chr2_1:29252422-29252441 Msa0204300:intron 45.0%
GTCAAGACGTGCTTCTTTGT+AGG + chr2_1:29252480-29252499 Msa0204300:intron 45.0%
AAGACGTGCTTCTTTGTAGG+AGG + chr2_1:29252483-29252502 Msa0204300:intron 45.0%
TTGAAAACCTCTGATGAGCC+AGG + chr2_1:29252585-29252604 Msa0204300:intron 45.0%
AGGACCCTACAATTGATACC+TGG - chr2_1:29252770-29252789 None:intergenic 45.0%
GCTTCGACCAATTCGATCAA+CGG + chr2_1:29252855-29252874 Msa0204300:intron 45.0%
ATACGTATGTGCAGGGTTGT+TGG + chr2_1:29253062-29253081 Msa0204300:intron 45.0%
TGCAAATCCTATGCGGAGAT+TGG - chr2_1:29253129-29253148 None:intergenic 45.0%
!! TCGAGGAAACATGATAGCGG+GGG - chr2_1:29249410-29249429 None:intergenic 50.0%
! CTCGAGGAAACATGATAGCG+GGG - chr2_1:29249411-29249430 None:intergenic 50.0%
! GACATCTGAAGCATGACTCG+AGG - chr2_1:29249427-29249446 None:intergenic 50.0%
!! AAATACGCTGGTGCCGTTGT+TGG + chr2_1:29249590-29249609 Msa0204300:five_prime_UTR 50.0%
!! CTGTGCTGCAATAGTGGACT+CGG + chr2_1:29249687-29249706 Msa0204300:five_prime_UTR 50.0%
!! TGTGCTGCAATAGTGGACTC+GGG + chr2_1:29249688-29249707 Msa0204300:five_prime_UTR 50.0%
GACTATCCACCTACGAAGCA+CGG + chr2_1:29249968-29249987 Msa0204300:five_prime_UTR 50.0%
TGTTCGTATCCGTGCTTCGT+AGG - chr2_1:29249980-29249999 None:intergenic 50.0%
ACGAAGCACGGATACGAACA+CGG + chr2_1:29249980-29249999 Msa0204300:five_prime_UTR 50.0%
TATGTGTCAGTGTCCGACAC+CGG + chr2_1:29250059-29250078 Msa0204300:five_prime_UTR 50.0%
GTGTGAGAGGCTTCCAAATC+CGG + chr2_1:29250879-29250898 Msa0204300:CDS 50.0%
GTTTCCTCTCTCTCCAGAAC+AGG + chr2_1:29250975-29250994 Msa0204300:CDS 50.0%
!! CAAACCTGTTCTGGAGAGAG+AGG - chr2_1:29250982-29251001 None:intergenic 50.0%
GACTACGCCAATCTCCGTAT+AGG + chr2_1:29251499-29251518 Msa0204300:CDS 50.0%
!! CTGCACTCGCTGTTTCTATG+TGG - chr2_1:29252045-29252064 None:intergenic 50.0%
TTGAAGTGGACACTACGGTG+GGG + chr2_1:29252334-29252353 Msa0204300:intron 50.0%
!! TGCGATGCTTTAGAGTCTCG+TGG - chr2_1:29252419-29252438 None:intergenic 50.0%
AAACCTCTGATGAGCCAGGA+AGG + chr2_1:29252589-29252608 Msa0204300:intron 50.0%
AACCTCTGATGAGCCAGGAA+GGG + chr2_1:29252590-29252609 Msa0204300:intron 50.0%
TCTACAACAGACAAGCGAGG+CGG - chr2_1:29252625-29252644 None:intergenic 50.0%
!! CGAGGAAACATGATAGCGGG+GGG - chr2_1:29249409-29249428 None:intergenic 55.0%
!! GTGCTGCAATAGTGGACTCG+GGG + chr2_1:29249689-29249708 Msa0204300:five_prime_UTR 55.0%
TCGTATCCGTGCTTCGTAGG+TGG - chr2_1:29249977-29249996 None:intergenic 55.0%
ATGTGTCAGTGTCCGACACC+GGG + chr2_1:29250060-29250079 Msa0204300:five_prime_UTR 55.0%
AATTAGGCGTGTCCCGGTGT+CGG - chr2_1:29250075-29250094 None:intergenic 55.0%
TCCTCGAATTAGGCGTGTCC+CGG - chr2_1:29250081-29250100 None:intergenic 55.0%
GCACGGACACTCCTCGAATT+AGG - chr2_1:29250091-29250110 None:intergenic 55.0%
GAGAGGCTTCCAAATCCGGT+TGG + chr2_1:29250883-29250902 Msa0204300:CDS 55.0%
TCTTGATGTGCCTTCCCCAC+AGG + chr2_1:29251298-29251317 Msa0204300:CDS 55.0%
CTTGATGTGCCTTCCCCACA+GGG + chr2_1:29251299-29251318 Msa0204300:CDS 55.0%
ATTACCAGAGGGGACCCTGT+GGG - chr2_1:29251316-29251335 None:intergenic 55.0%
TATTACCAGAGGGGACCCTG+TGG - chr2_1:29251317-29251336 None:intergenic 55.0%
TACGGTGGGGTTGACGCTAA+GGG + chr2_1:29252347-29252366 Msa0204300:intron 55.0%
TGGGGTTGACGCTAAGGGAT+GGG + chr2_1:29252352-29252371 Msa0204300:intron 55.0%
ACCTCTGATGAGCCAGGAAG+GGG + chr2_1:29252591-29252610 Msa0204300:intron 55.0%
GACTACGCCAATCTCCGCAT+AGG + chr2_1:29253119-29253138 Msa0204300:three_prime_UTR 55.0%
! GCGGGGGGCCTAACGTATTT+TGG - chr2_1:29249394-29249413 None:intergenic 60.0%
GGGTGGCTGTGCTGCAATAG+TGG + chr2_1:29249681-29249700 Msa0204300:five_prime_UTR 60.0%
ACCGGGACACGCCTAATTCG+AGG + chr2_1:29250077-29250096 Msa0204300:five_prime_UTR 60.0%
TTACCAGAGGGGACCCTGTG+GGG - chr2_1:29251315-29251334 None:intergenic 60.0%
CTACGGTGGGGTTGACGCTA+AGG + chr2_1:29252346-29252365 Msa0204300:intron 60.0%
GTGGGGTTGACGCTAAGGGA+TGG + chr2_1:29252351-29252370 Msa0204300:intron 60.0%
CCTCTGATGAGCCAGGAAGG+GGG + chr2_1:29252592-29252611 Msa0204300:intron 60.0%
ATGAGCCAGGAAGGGGGTTC+AGG + chr2_1:29252598-29252617 Msa0204300:intron 60.0%
TGAGCCAGGAAGGGGGTTCA+GGG + chr2_1:29252599-29252618 Msa0204300:intron 60.0%
CCCCCTTCCTGGCTCATCAG+AGG - chr2_1:29252595-29252614 None:intergenic 65.0%
CAGAGGGGACCCTGTGGGGA+AGG - chr2_1:29251311-29251330 None:intergenic 70.0%
CTTCCCCACAGGGTCCCCTC+TGG + chr2_1:29251309-29251328 Msa0204300:CDS 70.0%
GCGGCCCTGAACCCCCTTCC+TGG - chr2_1:29252606-29252625 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 29249240 29253160 29249240 ID=Msa0204300;Name=Msa0204300
chr2_1 mRNA 29249240 29253160 29249240 ID=Msa0204300-mRNA-1;Parent=Msa0204300;Name=Msa0204300-mRNA-1;_AED=0.05;_eAED=0.10;_QI=1165|0.75|0.6|1|0.5|0.4|5|84|125
chr2_1 exon 29249240 29250479 29249240 ID=Msa0204300-mRNA-1:exon:10487;Parent=Msa0204300-mRNA-1
chr2_1 exon 29250877 29250996 29250877 ID=Msa0204300-mRNA-1:exon:10488;Parent=Msa0204300-mRNA-1
chr2_1 exon 29251233 29251330 29251233 ID=Msa0204300-mRNA-1:exon:10489;Parent=Msa0204300-mRNA-1
chr2_1 exon 29251456 29251539 29251456 ID=Msa0204300-mRNA-1:exon:10490;Parent=Msa0204300-mRNA-1
chr2_1 exon 29253076 29253160 29253076 ID=Msa0204300-mRNA-1:exon:10491;Parent=Msa0204300-mRNA-1
chr2_1 five_prime_UTR 29249240 29250404 29249240 ID=Msa0204300-mRNA-1:five_prime_utr;Parent=Msa0204300-mRNA-1
chr2_1 CDS 29250405 29250479 29250405 ID=Msa0204300-mRNA-1:cds;Parent=Msa0204300-mRNA-1
chr2_1 CDS 29250877 29250996 29250877 ID=Msa0204300-mRNA-1:cds;Parent=Msa0204300-mRNA-1
chr2_1 CDS 29251233 29251330 29251233 ID=Msa0204300-mRNA-1:cds;Parent=Msa0204300-mRNA-1
chr2_1 CDS 29251456 29251539 29251456 ID=Msa0204300-mRNA-1:cds;Parent=Msa0204300-mRNA-1
chr2_1 CDS 29253076 29253076 29253076 ID=Msa0204300-mRNA-1:cds;Parent=Msa0204300-mRNA-1
chr2_1 three_prime_UTR 29253077 29253160 29253077 ID=Msa0204300-mRNA-1:three_prime_utr;Parent=Msa0204300-mRNA-1
Gene Sequence

>Msa0204300

ATGGTTGTCCTTTGGATGCAAAATCAGATTAAGCAAAGCAATGTAAAGGAAAAAGTATTAAAATATGTCGATGAGCTGTGTGAGAGGCTTCCAAATCCGGTTGGACAATCGTTTATAAACTGCAGTAGTGTCTCAGAAATGCCAAACATTACATTCACATTTGGAAACAAGTTGTTTCCTCTCTCTCCAGAACAGTATGTTCTTAGAGTTGAATCAGACGATGAAGATTGTTCTCCTGTGTGCTATAGCGGTTTTGTCGCTCTTGATGTGCCTTCCCCACAGGGTCCCCTCTGGGTTGTTGGAGATATTTTCTTGCAGGCATATCATACAGTGTTCGACTACGCCAATCTCCGTATAGGATTTGCAGAATCTGCATAG
Protein sequence

>Msa0204300

MVVLWMQNQIKQSNVKEKVLKYVDELCERLPNPVGQSFINCSSVSEMPNITFTFGNKLFPLSPEQYVLRVESDDEDCSPVCYSGFVALDVPSPQGPLWVVGDIFLQAYHTVFDYANLRIGFAESA*