AlfalfaGEDB | Msa0209970

M. sativa cultivar zhongmu-4 / Msa0209970

KEGG: K02108|K02126|K02132|K02262|K02967|K18757
GO: GO:0000276 GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0005753 GO:0006091 GO:0006119 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006839 GO:0006950 GO:0006996 GO:0007005 GO:0007006 GO:0007007 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0008324 GO:0008340 GO:0008553 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009628 GO:0009987 GO:0010259 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0015672 GO:0015985 GO:0015986 GO:0016020 GO:0016043 GO:0016310 GO:0016462 GO:0016469 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019725 GO:0019829 GO:0019866 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0031090 GO:0031966 GO:0031967 GO:0031975 GO:0032501 GO:0032502 GO:0032991 GO:0033177 GO:0034220 GO:0034641 GO:0034654 GO:0036296 GO:0036442 GO:0040011 GO:0042407 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0042776 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0044769 GO:0045259 GO:0045263 GO:0046034 GO:0046390 GO:0046483 GO:0046716 GO:0046907 GO:0046933 GO:0048856 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055085 GO:0055086 GO:0055093 GO:0060249 GO:0061024 GO:0065007 GO:0065008 GO:0070050 GO:0070482 GO:0071704 GO:0071840 GO:0072521 GO:0072522 GO:0090407 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0098796 GO:0098798 GO:0098800 GO:0099131 GO:0099132 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600 GO:1990542
PFAM: PF10250.12

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0209970	XP_003595703.2	98.662	299	4	0	1	299	222	520	0.0	617

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0209970	sp\|Q4V398\|RRT1_ARATH	75.261	287	71	0	1	287	213	499	1.67e-165	472

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0209970	G7IT24	98.662	299	4	0	1	299	222	520	0.0	617

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0039250	Msa0209970	0.800206	1.606743e-48	-8.615850e-47
Msa0142950	Msa0209970	0.807443	5.043087e-50	-8.615850e-47
Msa0209970	Msa0584550	0.805587	1.242452e-49	-8.615850e-47
Msa0209970	Msa1028290	0.803372	3.596942e-49	-8.615850e-47
Msa0209970	Msa1112390	0.807263	5.505963e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0209970	MtrunA17_Chr2g0307971	98.662	299	4	0	1	299	222	520	0.0	617
Msa0209970	MtrunA17_Chr4g0061781	88.591	298	32	1	1	298	223	518	0.0	549
Msa0209970	MtrunA17_Chr6g0455571	79.381	291	60	0	1	291	222	512	5.57e-177	498
Msa0209970	MtrunA17_Chr5g0417871	64.505	293	104	0	1	293	191	483	8.45e-142	409
Msa0209970	MtrunA17_Chr4g0042711	63.300	297	108	1	1	296	203	499	3.84e-140	405
Msa0209970	MtrunA17_Chr5g0436321	48.763	283	142	3	1	282	264	544	5.35e-90	278
Msa0209970	MtrunA17_Chr4g0008451	49.117	283	141	3	1	282	335	615	6.83e-89	278
Msa0209970	MtrunA17_Chr5g0426591	46.980	298	150	3	1	290	217	514	8.03e-88	271
Msa0209970	MtrunA17_Chr4g0070551	48.944	284	140	4	1	282	331	611	1.66e-87	273
Msa0209970	MtrunA17_Chr3g0129981	46.441	295	149	5	1	286	216	510	1.70e-86	268
Msa0209970	MtrunA17_Chr3g0132371	47.651	298	148	4	1	290	244	541	1.96e-86	268
Msa0209970	MtrunA17_Chr3g0143821	41.463	287	156	6	1	286	216	491	9.49e-62	205
Msa0209970	MtrunA17_Chr5g0399311	41.176	289	153	8	1	286	230	504	5.32e-59	196
Msa0209970	MtrunA17_Chr5g0448581	39.465	299	161	6	1	294	223	506	1.61e-58	196
Msa0209970	MtrunA17_Chr4g0056061	41.762	261	145	5	1	260	214	468	1.91e-57	193
Msa0209970	MtrunA17_Chr5g0412251	34.177	316	168	7	1	288	199	502	4.65e-53	181
Msa0209970	MtrunA17_Chr1g0204121	37.457	291	168	6	2	289	197	476	1.42e-52	179
Msa0209970	MtrunA17_Chr3g0123521	36.900	271	154	5	2	260	261	526	5.15e-49	171
Msa0209970	MtrunA17_Chr3g0132951	33.121	314	170	7	1	286	213	514	7.93e-49	169
Msa0209970	MtrunA17_Chr3g0087291	36.719	256	156	3	7	260	282	533	1.04e-48	170
Msa0209970	MtrunA17_Chr3g0077321	35.644	303	168	8	6	293	261	551	5.09e-48	168
Msa0209970	MtrunA17_Chr4g0043911	35.275	309	173	8	6	299	264	560	9.50e-48	167
Msa0209970	MtrunA17_Chr1g0160781	37.729	273	144	7	26	283	282	543	1.80e-47	166
Msa0209970	MtrunA17_Chr7g0240241	59.167	120	45	1	54	169	1	120	3.91e-46	152
Msa0209970	MtrunA17_Chr4g0010511	36.134	238	147	2	25	260	359	593	2.53e-45	162
Msa0209970	MtrunA17_Chr5g0424241	35.410	305	174	9	1	289	266	563	6.48e-45	160
Msa0209970	MtrunA17_Chr1g0204141	34.354	294	176	8	2	289	197	479	7.87e-44	156
Msa0209970	MtrunA17_Chr2g0284071	35.517	290	148	7	1	253	325	612	2.24e-43	157
Msa0209970	MtrunA17_Chr4g0075311	34.251	327	164	10	1	286	274	590	6.84e-43	155
Msa0209970	MtrunA17_Chr4g0003561	35.065	231	132	6	31	257	207	423	8.26e-31	120
Msa0209970	MtrunA17_Chr3g0127811	37.234	188	96	7	2	174	162	342	1.23e-23	100
Msa0209970	MtrunA17_Chr1g0146501	37.234	188	96	7	2	174	202	382	2.20e-23	100
Msa0209970	MtrunA17_Chr1g0172911	84.000	50	8	0	7	56	80	129	1.32e-19	82.8
Msa0209970	MtrunA17_Chr7g0220381	30.729	192	105	6	1	174	209	390	1.39e-19	89.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0209970	AT5G15740.1	75.261	287	71	0	1	287	213	499	1.69e-166	472
Msa0209970	AT3G02250.1	73.379	293	78	0	1	293	213	505	3.90e-163	464
Msa0209970	AT1G14020.1	65.035	286	100	0	1	286	197	482	7.78e-142	409
Msa0209970	AT2G03280.1	64.437	284	100	1	4	286	197	480	5.64e-138	399
Msa0209970	AT2G03280.2	64.437	284	100	1	4	286	224	507	1.21e-137	399
Msa0209970	AT2G37980.1	47.000	300	154	5	1	297	329	626	1.78e-87	274
Msa0209970	AT5G64600.1	46.667	300	151	4	1	291	219	518	9.09e-87	269
Msa0209970	AT3G54100.1	47.917	288	146	4	1	286	325	610	1.55e-86	271
Msa0209970	AT5G35570.1	45.847	301	158	5	1	298	354	652	5.43e-84	265
Msa0209970	AT5G01100.1	46.341	287	146	4	1	282	319	602	3.26e-83	263
Msa0209970	AT1G22460.1	43.729	295	158	3	4	290	266	560	1.20e-80	254
Msa0209970	AT1G20550.1	40.878	296	155	6	1	291	213	493	3.93e-65	214
Msa0209970	AT1G76270.1	42.268	291	148	6	1	286	210	485	4.64e-64	211
Msa0209970	AT4G38390.1	42.014	288	153	6	1	286	229	504	2.25e-63	209
Msa0209970	AT4G16650.2	39.655	290	160	7	1	288	228	504	2.63e-56	190
Msa0209970	AT4G16650.1	39.655	290	160	7	1	288	228	504	2.63e-56	190
Msa0209970	AT4G24530.1	35.350	314	163	7	1	286	214	515	5.27e-53	181
Msa0209970	AT5G65470.1	33.544	316	168	7	1	286	198	501	5.05e-52	178
Msa0209970	AT1G04910.1	37.931	290	166	6	2	288	197	475	1.67e-50	174
Msa0209970	AT5G63390.1	41.228	228	131	2	10	235	277	503	1.85e-50	175
Msa0209970	AT3G26370.1	36.162	271	156	5	2	260	269	534	1.47e-49	172
Msa0209970	AT1G38065.2	35.762	302	171	9	1	286	135	429	1.63e-48	168
Msa0209970	AT1G38065.1	35.762	302	171	9	1	286	145	439	2.33e-48	168
Msa0209970	AT2G44500.1	35.581	267	166	3	2	266	282	544	1.13e-47	168
Msa0209970	AT1G38131.1	35.762	302	171	9	1	286	264	558	1.37e-47	168
Msa0209970	AT3G07900.1	34.836	244	154	2	25	266	315	555	8.01e-47	166
Msa0209970	AT1G35510.1	35.915	284	155	8	25	293	291	562	1.86e-45	162
Msa0209970	AT1G11990.1	39.015	264	149	6	1	254	300	561	2.66e-45	162
Msa0209970	AT1G29200.3	34.602	289	151	7	1	253	105	391	3.83e-45	158
Msa0209970	AT1G29200.4	34.602	289	151	7	1	253	179	465	1.35e-44	158
Msa0209970	AT1G29200.1	34.602	289	151	7	1	253	179	465	1.35e-44	158
Msa0209970	AT1G29200.2	34.602	289	151	7	1	253	295	581	6.37e-44	158
Msa0209970	AT2G01480.1	33.557	298	170	9	6	288	268	552	1.33e-43	157
Msa0209970	AT1G14970.3	34.471	293	165	8	6	283	123	403	4.09e-43	152
Msa0209970	AT1G14970.2	34.471	293	165	8	6	283	149	429	8.15e-43	152
Msa0209970	AT1G62330.1	32.722	327	170	10	1	286	331	648	1.24e-42	155
Msa0209970	AT1G14970.1	34.471	293	165	8	6	283	269	549	3.59e-42	153
Msa0209970	AT2G01480.2	35.081	248	145	5	6	240	268	512	2.66e-40	147
Msa0209970	AT1G11990.2	34.219	301	149	6	1	254	285	583	4.32e-40	147
Msa0209970	AT1G52630.2	34.906	212	128	5	31	241	118	320	1.61e-30	118
Msa0209970	AT1G52630.1	34.906	212	128	5	31	241	206	408	9.13e-30	117
Msa0209970	AT3G03810.2	36.709	158	89	4	25	174	227	381	3.23e-25	105
Msa0209970	AT3G03810.1	36.709	158	89	4	25	174	227	381	3.23e-25	105
Msa0209970	AT3G30300.2	35.032	157	93	4	25	174	238	392	4.36e-19	87.8
Msa0209970	AT3G30300.1	35.032	157	93	4	25	174	238	392	4.36e-19	87.8
Msa0209970	AT2G44500.2	37.600	125	75	2	2	124	282	405	1.05e-15	77.4

Find 103 sgRNAs with CRISPR-Local

Find 320 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCAAGTTAGTTACCAATTT+TGG	0.113273	2_1:-49646039	None:intergenic
CTCTAAAGATTGCCCATTAT+TGG	0.145525	2_1:-49645509	None:intergenic
ATATTTATATATCATATATT+AGG	0.173153	2_1:-49647338	None:intergenic
CCATTTGAGAGGAATGATTT+TGG	0.220189	2_1:-49646188	None:intergenic
CATTCATATACACGATTTCT+TGG	0.233826	2_1:+49647258	Msa0209970:three_prime_UTR
GAGCATCAGTTCGATTTAAA+TGG	0.241996	2_1:-49645480	None:intergenic
TAGAAATAATCCTCTTCTTT+TGG	0.250716	2_1:-49646860	None:intergenic
CAGCTTTCTGTTCAATAAAA+TGG	0.263568	2_1:-49646711	None:intergenic
GATGCTCGGCTGGCCAATAA+TGG	0.279707	2_1:+49645496	Msa0209970:CDS
CCAATTCCCTGCAGATATCT+TGG	0.305050	2_1:+49646677	Msa0209970:intron
GTGGAGTGTTTGGAAAATAA+TGG	0.314808	2_1:+49646947	Msa0209970:three_prime_UTR
ATGATCCAGCAAAATTAAAA+TGG	0.315935	2_1:-49647417	None:intergenic
AGAAGTTACCCTGCCATTAC+AGG	0.328579	2_1:+49647195	Msa0209970:three_prime_UTR
AGGAGACTGCCCTTGCGCTT+AGG	0.333762	2_1:+49645923	Msa0209970:CDS
TTCGTGGAAGCTTTATTCTT+TGG	0.333912	2_1:+49647290	Msa0209970:three_prime_UTR
AGCAAAGAACTATCCAAAAT+TGG	0.339773	2_1:+49646026	Msa0209970:CDS
TGATATATAAATATATTATA+AGG	0.340309	2_1:+49647347	Msa0209970:three_prime_UTR
GATTGTTCCAAACATTCATC+TGG	0.342222	2_1:-49646890	None:intergenic
TATTTATATATCATATATTA+GGG	0.345676	2_1:-49647337	None:intergenic
TCCTACATACGATGGAAATA+TGG	0.351400	2_1:+49646254	Msa0209970:CDS
GGAGACTGCCCTTGCGCTTA+GGG	0.383114	2_1:+49645924	Msa0209970:CDS
TACAGCTTAAGTGGAGTGTT+TGG	0.385116	2_1:+49646937	Msa0209970:exon
ACACAAGTGGAGCATTGAAC+TGG	0.387579	2_1:+49646756	Msa0209970:CDS
CAATTCCCTGCAGATATCTT+GGG	0.394274	2_1:+49646678	Msa0209970:intron
TTCGTCACTGTTGCAACCTT+GGG	0.405250	2_1:-49645692	None:intergenic
AAAGCTGACAGCATTTAGTA+TGG	0.405419	2_1:+49647031	Msa0209970:three_prime_UTR
CTTCGTCACTGTTGCAACCT+TGG	0.411586	2_1:-49645693	None:intergenic
TGCCAATCCAGATGAATGTT+TGG	0.411926	2_1:+49646883	Msa0209970:CDS
ATTGCAGCTGGTGAAATATA+TGG	0.413590	2_1:+49645979	Msa0209970:CDS
CCAAAATCATTCCTCTCAAA+TGG	0.415466	2_1:+49646188	Msa0209970:CDS
TACTCCTCAGATTGAGGAGT+TGG	0.419090	2_1:+49645576	Msa0209970:CDS
TTCCTCTCAAATGGCAGCAT+TGG	0.420556	2_1:+49646197	Msa0209970:CDS
TTCCAAACATTCATCTGGAT+TGG	0.428075	2_1:-49646885	None:intergenic
TGGATAGTTCTTTGCTAGAC+TGG	0.428159	2_1:-49646019	None:intergenic
AGCGCAAGGGCAGTCTCCTC+AGG	0.443865	2_1:-49645919	None:intergenic
TGCTCTCACCCTGTAATGGC+AGG	0.447295	2_1:-49647204	None:intergenic
TCTGATTTGAAAAGAAAAGA+TGG	0.451300	2_1:+49645880	Msa0209970:CDS
ACTGTGTAACTTAGTATTTG+TGG	0.453969	2_1:-49647002	None:intergenic
GATCTGCTCTCACCCTGTAA+TGG	0.454000	2_1:-49647208	None:intergenic
GCAGTCTCCTCAGGTGTTAA+CGG	0.454965	2_1:-49645910	None:intergenic
GCGATGACCTTCGACTACTT+TGG	0.458899	2_1:-49646277	None:intergenic
GTTGAGTAGTACAGCTTAAG+TGG	0.460193	2_1:+49646928	Msa0209970:exon
ATGCTCGGCTGGCCAATAAT+GGG	0.462952	2_1:+49645497	Msa0209970:CDS
AAATCGAACTGATGCTCGGC+TGG	0.463745	2_1:+49645486	Msa0209970:CDS
TAGATTTACTCCTCAGATTG+AGG	0.463836	2_1:+49645570	Msa0209970:CDS
GCAGATATGCTTATCCTTGG+TGG	0.469607	2_1:+49645839	Msa0209970:intron
AAACGCTAAGAAAATTAGCC+AGG	0.480150	2_1:+49647125	Msa0209970:three_prime_UTR
AAAGATTGGTAATCCCTGAT+AGG	0.482081	2_1:+49646837	Msa0209970:CDS
GATTGAGGAGTTGGGCAGAA+AGG	0.487892	2_1:+49645585	Msa0209970:CDS
GCCATATTTCCATCGTATGT+AGG	0.498188	2_1:-49646255	None:intergenic
GACTGCTAGAAGAAGGGTTG+GGG	0.499332	2_1:+49647072	Msa0209970:three_prime_UTR
TTGGCAGATATGCTTATCCT+TGG	0.502259	2_1:+49645836	Msa0209970:intron
TGACTGCTAGAAGAAGGGTT+GGG	0.506228	2_1:+49647071	Msa0209970:three_prime_UTR
GCAATCTCATGCTGACCGCA+TGG	0.512538	2_1:+49646802	Msa0209970:CDS
ACTCCTCAGATTGAGGAGTT+GGG	0.517069	2_1:+49645577	Msa0209970:CDS
TCATAGTGACTGCTAGAAGA+AGG	0.519117	2_1:+49647065	Msa0209970:three_prime_UTR
TTCTTGGAACATGCAGTTCG+TGG	0.521690	2_1:+49647274	Msa0209970:three_prime_UTR
ATCAATCTTCTGAGGCAGAA+TGG	0.531091	2_1:+49645610	Msa0209970:CDS
TCTCATGCTGACCGCATGGG+CGG	0.532585	2_1:+49646806	Msa0209970:CDS
ATTCAAATCTACATTGCAGC+TGG	0.537105	2_1:+49645967	Msa0209970:CDS
GAACTGGGAAGAATTCTCTT+CGG	0.540776	2_1:+49646772	Msa0209970:CDS
GGGCGGCGCAACGAAAAGAT+TGG	0.541350	2_1:+49646823	Msa0209970:CDS
GTGACTGCTAGAAGAAGGGT+TGG	0.545662	2_1:+49647070	Msa0209970:three_prime_UTR
TGTCAAAGGCCCTAAGCGCA+AGG	0.545999	2_1:-49645933	None:intergenic
ATTAAAATGGTAAGTCGTGA+AGG	0.546885	2_1:-49647404	None:intergenic
AAATCAAACTGCATTGTACT+CGG	0.550665	2_1:+49647232	Msa0209970:three_prime_UTR
TCGTACTCGTGTCCTCTAGT+TGG	0.555163	2_1:+49646972	Msa0209970:three_prime_UTR
GGTCAGAAAGGAAACACTGT+TGG	0.556548	2_1:+49646143	Msa0209970:intron
ATTTAAATCGAACTGATGCT+CGG	0.560307	2_1:+49645482	Msa0209970:CDS
TTGAAGAATTGACAAGGATG+AGG	0.561254	2_1:+49645716	Msa0209970:CDS
ATATGGTGGGAGTAGAAGAA+TGG	0.561862	2_1:+49645996	Msa0209970:CDS
GTCAGCATGAGATTGCTTCA+CGG	0.563051	2_1:-49646795	None:intergenic
CACAAGTGGAGCATTGAACT+GGG	0.566560	2_1:+49646757	Msa0209970:CDS
CCCTGATAGGCCAAAAGAAG+AGG	0.568413	2_1:+49646850	Msa0209970:CDS
ATCTTTGTTCCTACATACGA+TGG	0.573891	2_1:+49646246	Msa0209970:CDS
TATCATATATTAGGGAGAGA+AGG	0.574895	2_1:-49647329	None:intergenic
ACTTCATCTGAGGTATGAGA+TGG	0.580009	2_1:+49645645	Msa0209970:CDS
TGGAAGAACTGAAGTTCAGA+CGG	0.583636	2_1:-49646168	None:intergenic
CAATCTCATGCTGACCGCAT+GGG	0.586925	2_1:+49646803	Msa0209970:CDS
GAGGTATGAGATGGACATGT+TGG	0.593003	2_1:+49645654	Msa0209970:CDS
CTGCCCAACTCCTCAATCTG+AGG	0.593111	2_1:-49645580	None:intergenic
CATAGTGACTGCTAGAAGAA+GGG	0.594828	2_1:+49647066	Msa0209970:three_prime_UTR
TTGATAGATCAATACACAAG+TGG	0.597315	2_1:+49646743	Msa0209970:CDS
ACTTCTATACATGAATGTGA+TGG	0.597315	2_1:-49647178	None:intergenic
TGAATGTTTGGAACAATCAG+AGG	0.605546	2_1:+49646895	Msa0209970:CDS
GTCAAAGGCCCTAAGCGCAA+GGG	0.609740	2_1:-49645932	None:intergenic
ATCCAATGCTGCCATTTGAG+AGG	0.611241	2_1:-49646199	None:intergenic
GAAGTTACCCTGCCATTACA+GGG	0.613050	2_1:+49647196	Msa0209970:three_prime_UTR
AACGCTAAGAAAATTAGCCA+GGG	0.613156	2_1:+49647126	Msa0209970:three_prime_UTR
AAGTAGTCGAAGGTCATCGC+AGG	0.614371	2_1:+49646280	Msa0209970:CDS
CAGCATTTAGTATGGAAGAG+TGG	0.614574	2_1:+49647039	Msa0209970:three_prime_UTR
ACTGCTAGAAGAAGGGTTGG+GGG	0.620757	2_1:+49647073	Msa0209970:three_prime_UTR
TAAGAGCACTAAAATTGACT+CGG	0.629487	2_1:-49645549	None:intergenic
CTGCTAGAAGAAGGGTTGGG+GGG	0.635056	2_1:+49647074	Msa0209970:three_prime_UTR
GCTCTCACCCTGTAATGGCA+GGG	0.638471	2_1:-49647203	None:intergenic
GAAAGGTTATCAATCTTCTG+AGG	0.645126	2_1:+49645602	Msa0209970:CDS
AATATGGCCAAAGTAGTCGA+AGG	0.658747	2_1:+49646270	Msa0209970:CDS
TGTGGTAGAAAGCCAACTAG+AGG	0.659531	2_1:-49646984	None:intergenic
ACGAAGTTGAAGAATTGACA+AGG	0.668646	2_1:+49645710	Msa0209970:CDS
TTTATGTCCGTTAACACCTG+AGG	0.671972	2_1:+49645903	Msa0209970:CDS
CAGCTGGTGAAATATATGGT+GGG	0.691273	2_1:+49645983	Msa0209970:CDS
GCAGCTGGTGAAATATATGG+TGG	0.713694	2_1:+49645982	Msa0209970:CDS
TCAAACTGCATTGTACTCGG+AGG	0.736871	2_1:+49647235	Msa0209970:three_prime_UTR

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CAAATTAAAATTTGAATAAT+TGG	-	chr2_1:49646385-49646404	None:intergenic	10.0%
!!	CAAATTAAAATTTGAATAAT+TGG	-	chr2_1:49646385-49646404	None:intergenic	10.0%
!!!	TTGTTTCTTTATATTTCTTT+AGG	+	chr2_1:49646432-49646451	Msa0209970:intron	15.0%
!!	ACTACAAATTATAACAAATA+AGG	-	chr2_1:49646625-49646644	None:intergenic	15.0%
!!!	CTAAGAAATTTTTTTATAGA+GGG	-	chr2_1:49647444-49647463	None:intergenic	15.0%
!!	TAAAAAAATTTCTTAGCATT+TGG	+	chr2_1:49647448-49647467	Msa0209970:three_prime_UTR	15.0%
!!!	TTGTTTCTTTATATTTCTTT+AGG	+	chr2_1:49646432-49646451	Msa0209970:intron	15.0%
!!	ACTACAAATTATAACAAATA+AGG	-	chr2_1:49646625-49646644	None:intergenic	15.0%
!!!	CTAAGAAATTTTTTTATAGA+GGG	-	chr2_1:49647444-49647463	None:intergenic	15.0%
!!	TAAAAAAATTTCTTAGCATT+TGG	+	chr2_1:49647448-49647467	Msa0209970:three_prime_UTR	15.0%
!!!	GATACAAACAATGATTTTAA+GGG	-	chr2_1:49646496-49646515	None:intergenic	20.0%
!!	ACAAATTATAACAAATAAGG+AGG	-	chr2_1:49646622-49646641	None:intergenic	20.0%
!!!	TTTTATATATAAACTAACCC+TGG	-	chr2_1:49647146-49647165	None:intergenic	20.0%
!!!	GCTAAGAAATTTTTTTATAG+AGG	-	chr2_1:49647445-49647464	None:intergenic	20.0%
!!!	GATACAAACAATGATTTTAA+GGG	-	chr2_1:49646496-49646515	None:intergenic	20.0%
!!	ACAAATTATAACAAATAAGG+AGG	-	chr2_1:49646622-49646641	None:intergenic	20.0%
!!!	TTTTATATATAAACTAACCC+TGG	-	chr2_1:49647146-49647165	None:intergenic	20.0%
!!!	GCTAAGAAATTTTTTTATAG+AGG	-	chr2_1:49647445-49647464	None:intergenic	20.0%
!	TGTACAGGAAATTTGAATTT+AGG	-	chr2_1:49645816-49645835	None:intergenic	25.0%
!	AATTCAAATTTCCTGTACAT+TGG	+	chr2_1:49645817-49645836	Msa0209970:intron	25.0%
!!!	ATTATTTTTTCTTTCCACCA+AGG	-	chr2_1:49645856-49645875	None:intergenic	25.0%
!!	TCTGATTTGAAAAGAAAAGA+TGG	+	chr2_1:49645880-49645899	Msa0209970:CDS	25.0%
!!!	AATGTTTTGATCAATGTCAA+AGG	-	chr2_1:49645950-49645969	None:intergenic	25.0%
!!!	TAATTTGTTTCTTCTACCAA+TGG	+	chr2_1:49646398-49646417	Msa0209970:intron	25.0%
!	AACAAGTTTACAATTACCAT+TGG	-	chr2_1:49646417-49646436	None:intergenic	25.0%
!!	GGATACAAACAATGATTTTA+AGG	-	chr2_1:49646497-49646516	None:intergenic	25.0%
!!	TAGAAATAATCCTCTTCTTT+TGG	-	chr2_1:49646863-49646882	None:intergenic	25.0%
!!	AAAAAAAAGTGTGGTAAAAG+AGG	-	chr2_1:49647382-49647401	None:intergenic	25.0%
!!!	ACTTACCATTTTAATTTTGC+TGG	+	chr2_1:49647412-49647431	Msa0209970:three_prime_UTR	25.0%
!	ATGATCCAGCAAAATTAAAA+TGG	-	chr2_1:49647420-49647439	None:intergenic	25.0%
!	TGTACAGGAAATTTGAATTT+AGG	-	chr2_1:49645816-49645835	None:intergenic	25.0%
!	AATTCAAATTTCCTGTACAT+TGG	+	chr2_1:49645817-49645836	Msa0209970:intron	25.0%
!!!	ATTATTTTTTCTTTCCACCA+AGG	-	chr2_1:49645856-49645875	None:intergenic	25.0%
!!	TCTGATTTGAAAAGAAAAGA+TGG	+	chr2_1:49645880-49645899	Msa0209970:CDS	25.0%
!!!	AATGTTTTGATCAATGTCAA+AGG	-	chr2_1:49645950-49645969	None:intergenic	25.0%
!!!	TAATTTGTTTCTTCTACCAA+TGG	+	chr2_1:49646398-49646417	Msa0209970:intron	25.0%
!	AACAAGTTTACAATTACCAT+TGG	-	chr2_1:49646417-49646436	None:intergenic	25.0%
!!	GGATACAAACAATGATTTTA+AGG	-	chr2_1:49646497-49646516	None:intergenic	25.0%
!!	TAGAAATAATCCTCTTCTTT+TGG	-	chr2_1:49646863-49646882	None:intergenic	25.0%
!!	AAAAAAAAGTGTGGTAAAAG+AGG	-	chr2_1:49647382-49647401	None:intergenic	25.0%
!!!	ACTTACCATTTTAATTTTGC+TGG	+	chr2_1:49647412-49647431	Msa0209970:three_prime_UTR	25.0%
!	ATGATCCAGCAAAATTAAAA+TGG	-	chr2_1:49647420-49647439	None:intergenic	25.0%
	ATTTAAATCGAACTGATGCT+CGG	+	chr2_1:49645482-49645501	Msa0209970:CDS	30.0%
!!	TAAGAGCACTAAAATTGACT+CGG	-	chr2_1:49645552-49645571	None:intergenic	30.0%
!	TCAGATGAAGTACCAAAAAT+GGG	-	chr2_1:49645636-49645655	None:intergenic	30.0%
	TGTTCCATAACTTTCATTCT+TGG	+	chr2_1:49645761-49645780	Msa0209970:intron	30.0%
	AACTCCAAGAATGAAAGTTA+TGG	-	chr2_1:49645768-49645787	None:intergenic	30.0%
	AGCAAAGAACTATCCAAAAT+TGG	+	chr2_1:49646026-49646045	Msa0209970:CDS	30.0%
!	CTCAAGTTAGTTACCAATTT+TGG	-	chr2_1:49646042-49646061	None:intergenic	30.0%
!	CAAAATTGGTAACTAACTTG+AGG	+	chr2_1:49646040-49646059	Msa0209970:intron	30.0%
!!!	CATTTGTGATTTTCTGAAAC+AGG	+	chr2_1:49646122-49646141	Msa0209970:intron	30.0%
	TATTCAGCAAAAAAACAGTC+AGG	-	chr2_1:49646535-49646554	None:intergenic	30.0%
	CAGCTTTCTGTTCAATAAAA+TGG	-	chr2_1:49646714-49646733	None:intergenic	30.0%
	TTGATAGATCAATACACAAG+TGG	+	chr2_1:49646743-49646762	Msa0209970:CDS	30.0%
	ACTGTGTAACTTAGTATTTG+TGG	-	chr2_1:49647005-49647024	None:intergenic	30.0%
	ACTTCTATACATGAATGTGA+TGG	-	chr2_1:49647181-49647200	None:intergenic	30.0%
	AAATCAAACTGCATTGTACT+CGG	+	chr2_1:49647232-49647251	Msa0209970:three_prime_UTR	30.0%
	CATTCATATACACGATTTCT+TGG	+	chr2_1:49647258-49647277	Msa0209970:three_prime_UTR	30.0%
	TATCATATATTAGGGAGAGA+AGG	-	chr2_1:49647332-49647351	None:intergenic	30.0%
	GTGAAGGATAAAAAAAAGTG+TGG	-	chr2_1:49647391-49647410	None:intergenic	30.0%
	ATTAAAATGGTAAGTCGTGA+AGG	-	chr2_1:49647407-49647426	None:intergenic	30.0%
	ATTTAAATCGAACTGATGCT+CGG	+	chr2_1:49645482-49645501	Msa0209970:CDS	30.0%
!!	TAAGAGCACTAAAATTGACT+CGG	-	chr2_1:49645552-49645571	None:intergenic	30.0%
!	TCAGATGAAGTACCAAAAAT+GGG	-	chr2_1:49645636-49645655	None:intergenic	30.0%
	TGTTCCATAACTTTCATTCT+TGG	+	chr2_1:49645761-49645780	Msa0209970:intron	30.0%
	AACTCCAAGAATGAAAGTTA+TGG	-	chr2_1:49645768-49645787	None:intergenic	30.0%
	AGCAAAGAACTATCCAAAAT+TGG	+	chr2_1:49646026-49646045	Msa0209970:CDS	30.0%
!	CTCAAGTTAGTTACCAATTT+TGG	-	chr2_1:49646042-49646061	None:intergenic	30.0%
!	CAAAATTGGTAACTAACTTG+AGG	+	chr2_1:49646040-49646059	Msa0209970:intron	30.0%
!!!	CATTTGTGATTTTCTGAAAC+AGG	+	chr2_1:49646122-49646141	Msa0209970:intron	30.0%
	TATTCAGCAAAAAAACAGTC+AGG	-	chr2_1:49646535-49646554	None:intergenic	30.0%
	CAGCTTTCTGTTCAATAAAA+TGG	-	chr2_1:49646714-49646733	None:intergenic	30.0%
	TTGATAGATCAATACACAAG+TGG	+	chr2_1:49646743-49646762	Msa0209970:CDS	30.0%
	ACTGTGTAACTTAGTATTTG+TGG	-	chr2_1:49647005-49647024	None:intergenic	30.0%
	ACTTCTATACATGAATGTGA+TGG	-	chr2_1:49647181-49647200	None:intergenic	30.0%
	AAATCAAACTGCATTGTACT+CGG	+	chr2_1:49647232-49647251	Msa0209970:three_prime_UTR	30.0%
	CATTCATATACACGATTTCT+TGG	+	chr2_1:49647258-49647277	Msa0209970:three_prime_UTR	30.0%
	TATCATATATTAGGGAGAGA+AGG	-	chr2_1:49647332-49647351	None:intergenic	30.0%
	GTGAAGGATAAAAAAAAGTG+TGG	-	chr2_1:49647391-49647410	None:intergenic	30.0%
	ATTAAAATGGTAAGTCGTGA+AGG	-	chr2_1:49647407-49647426	None:intergenic	30.0%
	GAGCATCAGTTCGATTTAAA+TGG	-	chr2_1:49645483-49645502	None:intergenic	35.0%
!	CTCTAAAGATTGCCCATTAT+TGG	-	chr2_1:49645512-49645531	None:intergenic	35.0%
	TAGATTTACTCCTCAGATTG+AGG	+	chr2_1:49645570-49645589	Msa0209970:CDS	35.0%
	GAAAGGTTATCAATCTTCTG+AGG	+	chr2_1:49645602-49645621	Msa0209970:CDS	35.0%
!	CTCAGATGAAGTACCAAAAA+TGG	-	chr2_1:49645637-49645656	None:intergenic	35.0%
!!!	CATTTTTGGTACTTCATCTG+AGG	+	chr2_1:49645635-49645654	Msa0209970:CDS	35.0%
	ACGAAGTTGAAGAATTGACA+AGG	+	chr2_1:49645710-49645729	Msa0209970:CDS	35.0%
	TTGAAGAATTGACAAGGATG+AGG	+	chr2_1:49645716-49645735	Msa0209970:CDS	35.0%
	CTTTCATTCTTGGAGTTGAA+AGG	+	chr2_1:49645771-49645790	Msa0209970:intron	35.0%
	ATTCAAATCTACATTGCAGC+TGG	+	chr2_1:49645967-49645986	Msa0209970:CDS	35.0%
	ATTGCAGCTGGTGAAATATA+TGG	+	chr2_1:49645979-49645998	Msa0209970:CDS	35.0%
	CATAAGGAAACCTAGGAATT+GGG	+	chr2_1:49646063-49646082	Msa0209970:intron	35.0%
	GAAAAAGAATCCCAATTCCT+AGG	-	chr2_1:49646076-49646095	None:intergenic	35.0%
!!!	TTTTCTGAAACAGGTCAGAA+AGG	+	chr2_1:49646131-49646150	Msa0209970:intron	35.0%
!	CCATTTGAGAGGAATGATTT+TGG	-	chr2_1:49646191-49646210	None:intergenic	35.0%
	CCAAAATCATTCCTCTCAAA+TGG	+	chr2_1:49646188-49646207	Msa0209970:CDS	35.0%
	ATCTTTGTTCCTACATACGA+TGG	+	chr2_1:49646246-49646265	Msa0209970:CDS	35.0%
	TCCTACATACGATGGAAATA+TGG	+	chr2_1:49646254-49646273	Msa0209970:CDS	35.0%
	ATTGGTTAACAAGAGCAAGA+GGG	-	chr2_1:49646367-49646386	None:intergenic	35.0%
	AATTGGTTAACAAGAGCAAG+AGG	-	chr2_1:49646368-49646387	None:intergenic	35.0%
!	AAAGATTGGTAATCCCTGAT+AGG	+	chr2_1:49646837-49646856	Msa0209970:CDS	35.0%
	TTCCAAACATTCATCTGGAT+TGG	-	chr2_1:49646888-49646907	None:intergenic	35.0%
	GATTGTTCCAAACATTCATC+TGG	-	chr2_1:49646893-49646912	None:intergenic	35.0%
!	TGAATGTTTGGAACAATCAG+AGG	+	chr2_1:49646895-49646914	Msa0209970:CDS	35.0%
!	GTGGAGTGTTTGGAAAATAA+TGG	+	chr2_1:49646947-49646966	Msa0209970:three_prime_UTR	35.0%
	AAAGCTGACAGCATTTAGTA+TGG	+	chr2_1:49647031-49647050	Msa0209970:three_prime_UTR	35.0%
	AAACGCTAAGAAAATTAGCC+AGG	+	chr2_1:49647125-49647144	Msa0209970:three_prime_UTR	35.0%
	AACGCTAAGAAAATTAGCCA+GGG	+	chr2_1:49647126-49647145	Msa0209970:three_prime_UTR	35.0%
!!	TTCGTGGAAGCTTTATTCTT+TGG	+	chr2_1:49647290-49647309	Msa0209970:three_prime_UTR	35.0%
	GAGAGAAGGCAAAAATAATG+AGG	-	chr2_1:49647318-49647337	None:intergenic	35.0%
	GAGCATCAGTTCGATTTAAA+TGG	-	chr2_1:49645483-49645502	None:intergenic	35.0%
!	CTCTAAAGATTGCCCATTAT+TGG	-	chr2_1:49645512-49645531	None:intergenic	35.0%
	TAGATTTACTCCTCAGATTG+AGG	+	chr2_1:49645570-49645589	Msa0209970:CDS	35.0%
	GAAAGGTTATCAATCTTCTG+AGG	+	chr2_1:49645602-49645621	Msa0209970:CDS	35.0%
!	CTCAGATGAAGTACCAAAAA+TGG	-	chr2_1:49645637-49645656	None:intergenic	35.0%
!!!	CATTTTTGGTACTTCATCTG+AGG	+	chr2_1:49645635-49645654	Msa0209970:CDS	35.0%
	ACGAAGTTGAAGAATTGACA+AGG	+	chr2_1:49645710-49645729	Msa0209970:CDS	35.0%
	TTGAAGAATTGACAAGGATG+AGG	+	chr2_1:49645716-49645735	Msa0209970:CDS	35.0%
	CTTTCATTCTTGGAGTTGAA+AGG	+	chr2_1:49645771-49645790	Msa0209970:intron	35.0%
	ATTCAAATCTACATTGCAGC+TGG	+	chr2_1:49645967-49645986	Msa0209970:CDS	35.0%
	ATTGCAGCTGGTGAAATATA+TGG	+	chr2_1:49645979-49645998	Msa0209970:CDS	35.0%
	CATAAGGAAACCTAGGAATT+GGG	+	chr2_1:49646063-49646082	Msa0209970:intron	35.0%
	GAAAAAGAATCCCAATTCCT+AGG	-	chr2_1:49646076-49646095	None:intergenic	35.0%
!!!	TTTTCTGAAACAGGTCAGAA+AGG	+	chr2_1:49646131-49646150	Msa0209970:intron	35.0%
!	CCATTTGAGAGGAATGATTT+TGG	-	chr2_1:49646191-49646210	None:intergenic	35.0%
	CCAAAATCATTCCTCTCAAA+TGG	+	chr2_1:49646188-49646207	Msa0209970:CDS	35.0%
	ATCTTTGTTCCTACATACGA+TGG	+	chr2_1:49646246-49646265	Msa0209970:CDS	35.0%
	TCCTACATACGATGGAAATA+TGG	+	chr2_1:49646254-49646273	Msa0209970:CDS	35.0%
	ATTGGTTAACAAGAGCAAGA+GGG	-	chr2_1:49646367-49646386	None:intergenic	35.0%
	AATTGGTTAACAAGAGCAAG+AGG	-	chr2_1:49646368-49646387	None:intergenic	35.0%
!	AAAGATTGGTAATCCCTGAT+AGG	+	chr2_1:49646837-49646856	Msa0209970:CDS	35.0%
	TTCCAAACATTCATCTGGAT+TGG	-	chr2_1:49646888-49646907	None:intergenic	35.0%
	GATTGTTCCAAACATTCATC+TGG	-	chr2_1:49646893-49646912	None:intergenic	35.0%
!	TGAATGTTTGGAACAATCAG+AGG	+	chr2_1:49646895-49646914	Msa0209970:CDS	35.0%
!	GTGGAGTGTTTGGAAAATAA+TGG	+	chr2_1:49646947-49646966	Msa0209970:three_prime_UTR	35.0%
	AAAGCTGACAGCATTTAGTA+TGG	+	chr2_1:49647031-49647050	Msa0209970:three_prime_UTR	35.0%
	AAACGCTAAGAAAATTAGCC+AGG	+	chr2_1:49647125-49647144	Msa0209970:three_prime_UTR	35.0%
	AACGCTAAGAAAATTAGCCA+GGG	+	chr2_1:49647126-49647145	Msa0209970:three_prime_UTR	35.0%
!!	TTCGTGGAAGCTTTATTCTT+TGG	+	chr2_1:49647290-49647309	Msa0209970:three_prime_UTR	35.0%
	GAGAGAAGGCAAAAATAATG+AGG	-	chr2_1:49647318-49647337	None:intergenic	35.0%
!!	ATCAATCTTCTGAGGCAGAA+TGG	+	chr2_1:49645610-49645629	Msa0209970:CDS	40.0%
!	ACTTCATCTGAGGTATGAGA+TGG	+	chr2_1:49645645-49645664	Msa0209970:CDS	40.0%
!	GATGGACATGTTGGCATTTT+CGG	+	chr2_1:49645663-49645682	Msa0209970:CDS	40.0%
!	ATGGACATGTTGGCATTTTC+GGG	+	chr2_1:49645664-49645683	Msa0209970:CDS	40.0%
	AAGCATATCTGCCAATGTAC+AGG	-	chr2_1:49645831-49645850	None:intergenic	40.0%
	TTGGCAGATATGCTTATCCT+TGG	+	chr2_1:49645836-49645855	Msa0209970:intron	40.0%
	TTTATGTCCGTTAACACCTG+AGG	+	chr2_1:49645903-49645922	Msa0209970:CDS	40.0%
	CAGCTGGTGAAATATATGGT+GGG	+	chr2_1:49645983-49646002	Msa0209970:CDS	40.0%
!	ATATGGTGGGAGTAGAAGAA+TGG	+	chr2_1:49645996-49646015	Msa0209970:CDS	40.0%
	TGGATAGTTCTTTGCTAGAC+TGG	-	chr2_1:49646022-49646041	None:intergenic	40.0%
	GGTAACTAACTTGAGGCATA+AGG	+	chr2_1:49646047-49646066	Msa0209970:intron	40.0%
	GCATAAGGAAACCTAGGAAT+TGG	+	chr2_1:49646062-49646081	Msa0209970:intron	40.0%
	TGGAAGAACTGAAGTTCAGA+CGG	-	chr2_1:49646171-49646190	None:intergenic	40.0%
	GCCATATTTCCATCGTATGT+AGG	-	chr2_1:49646258-49646277	None:intergenic	40.0%
	AATATGGCCAAAGTAGTCGA+AGG	+	chr2_1:49646270-49646289	Msa0209970:CDS	40.0%
	CAATTCCCTGCAGATATCTT+GGG	+	chr2_1:49646678-49646697	Msa0209970:intron	40.0%
	TTGAACCCAAGATATCTGCA+GGG	-	chr2_1:49646686-49646705	None:intergenic	40.0%
	GAACTGGGAAGAATTCTCTT+CGG	+	chr2_1:49646772-49646791	Msa0209970:CDS	40.0%
	TGCCAATCCAGATGAATGTT+TGG	+	chr2_1:49646883-49646902	Msa0209970:CDS	40.0%
	GTTGAGTAGTACAGCTTAAG+TGG	+	chr2_1:49646928-49646947	Msa0209970:exon	40.0%
!	TACAGCTTAAGTGGAGTGTT+TGG	+	chr2_1:49646937-49646956	Msa0209970:exon	40.0%
	CAGCATTTAGTATGGAAGAG+TGG	+	chr2_1:49647039-49647058	Msa0209970:three_prime_UTR	40.0%
	TCATAGTGACTGCTAGAAGA+AGG	+	chr2_1:49647065-49647084	Msa0209970:three_prime_UTR	40.0%
	CATAGTGACTGCTAGAAGAA+GGG	+	chr2_1:49647066-49647085	Msa0209970:three_prime_UTR	40.0%
!!	ATCAATCTTCTGAGGCAGAA+TGG	+	chr2_1:49645610-49645629	Msa0209970:CDS	40.0%
!	ACTTCATCTGAGGTATGAGA+TGG	+	chr2_1:49645645-49645664	Msa0209970:CDS	40.0%
!	GATGGACATGTTGGCATTTT+CGG	+	chr2_1:49645663-49645682	Msa0209970:CDS	40.0%
!	ATGGACATGTTGGCATTTTC+GGG	+	chr2_1:49645664-49645683	Msa0209970:CDS	40.0%
	AAGCATATCTGCCAATGTAC+AGG	-	chr2_1:49645831-49645850	None:intergenic	40.0%
	TTGGCAGATATGCTTATCCT+TGG	+	chr2_1:49645836-49645855	Msa0209970:intron	40.0%
	TTTATGTCCGTTAACACCTG+AGG	+	chr2_1:49645903-49645922	Msa0209970:CDS	40.0%
	CAGCTGGTGAAATATATGGT+GGG	+	chr2_1:49645983-49646002	Msa0209970:CDS	40.0%
!	ATATGGTGGGAGTAGAAGAA+TGG	+	chr2_1:49645996-49646015	Msa0209970:CDS	40.0%
	TGGATAGTTCTTTGCTAGAC+TGG	-	chr2_1:49646022-49646041	None:intergenic	40.0%
	GGTAACTAACTTGAGGCATA+AGG	+	chr2_1:49646047-49646066	Msa0209970:intron	40.0%
	GCATAAGGAAACCTAGGAAT+TGG	+	chr2_1:49646062-49646081	Msa0209970:intron	40.0%
	TGGAAGAACTGAAGTTCAGA+CGG	-	chr2_1:49646171-49646190	None:intergenic	40.0%
	GCCATATTTCCATCGTATGT+AGG	-	chr2_1:49646258-49646277	None:intergenic	40.0%
	AATATGGCCAAAGTAGTCGA+AGG	+	chr2_1:49646270-49646289	Msa0209970:CDS	40.0%
	CAATTCCCTGCAGATATCTT+GGG	+	chr2_1:49646678-49646697	Msa0209970:intron	40.0%
	TTGAACCCAAGATATCTGCA+GGG	-	chr2_1:49646686-49646705	None:intergenic	40.0%
	GAACTGGGAAGAATTCTCTT+CGG	+	chr2_1:49646772-49646791	Msa0209970:CDS	40.0%
	TGCCAATCCAGATGAATGTT+TGG	+	chr2_1:49646883-49646902	Msa0209970:CDS	40.0%
	GTTGAGTAGTACAGCTTAAG+TGG	+	chr2_1:49646928-49646947	Msa0209970:exon	40.0%
!	TACAGCTTAAGTGGAGTGTT+TGG	+	chr2_1:49646937-49646956	Msa0209970:exon	40.0%
	CAGCATTTAGTATGGAAGAG+TGG	+	chr2_1:49647039-49647058	Msa0209970:three_prime_UTR	40.0%
	TCATAGTGACTGCTAGAAGA+AGG	+	chr2_1:49647065-49647084	Msa0209970:three_prime_UTR	40.0%
	CATAGTGACTGCTAGAAGAA+GGG	+	chr2_1:49647066-49647085	Msa0209970:three_prime_UTR	40.0%
	TACTCCTCAGATTGAGGAGT+TGG	+	chr2_1:49645576-49645595	Msa0209970:CDS	45.0%
	ACTCCTCAGATTGAGGAGTT+GGG	+	chr2_1:49645577-49645596	Msa0209970:CDS	45.0%
	GAGGTATGAGATGGACATGT+TGG	+	chr2_1:49645654-49645673	Msa0209970:CDS	45.0%
	TTCGTCACTGTTGCAACCTT+GGG	-	chr2_1:49645695-49645714	None:intergenic	45.0%
	GCAGATATGCTTATCCTTGG+TGG	+	chr2_1:49645839-49645858	Msa0209970:intron	45.0%
	GCAGCTGGTGAAATATATGG+TGG	+	chr2_1:49645982-49646001	Msa0209970:CDS	45.0%
	CTTGAGGCATAAGGAAACCT+AGG	+	chr2_1:49646056-49646075	Msa0209970:intron	45.0%
	GGTCAGAAAGGAAACACTGT+TGG	+	chr2_1:49646143-49646162	Msa0209970:intron	45.0%
	TTCCTCTCAAATGGCAGCAT+TGG	+	chr2_1:49646197-49646216	Msa0209970:CDS	45.0%
!	ATCCAATGCTGCCATTTGAG+AGG	-	chr2_1:49646202-49646221	None:intergenic	45.0%
	AGGTCATCGCAGGTAAAGTT+CGG	+	chr2_1:49646290-49646309	Msa0209970:intron	45.0%
	TAACAAGAGCAAGAGGGAGT+AGG	-	chr2_1:49646361-49646380	None:intergenic	45.0%
	GTCAGGTCATTGAGCAAAAG+TGG	-	chr2_1:49646518-49646537	None:intergenic	45.0%
	AGGGTAAAGGTCATGTCACT+TGG	-	chr2_1:49646657-49646676	None:intergenic	45.0%
	GATATCTGCAGGGAATTGGA+GGG	-	chr2_1:49646676-49646695	None:intergenic	45.0%
	AGATATCTGCAGGGAATTGG+AGG	-	chr2_1:49646677-49646696	None:intergenic	45.0%
	CCAAGATATCTGCAGGGAAT+TGG	-	chr2_1:49646680-49646699	None:intergenic	45.0%
	CCAATTCCCTGCAGATATCT+TGG	+	chr2_1:49646677-49646696	Msa0209970:intron	45.0%
	CTTGAACCCAAGATATCTGC+AGG	-	chr2_1:49646687-49646706	None:intergenic	45.0%
!!	ACACAAGTGGAGCATTGAAC+TGG	+	chr2_1:49646756-49646775	Msa0209970:CDS	45.0%
!!	CACAAGTGGAGCATTGAACT+GGG	+	chr2_1:49646757-49646776	Msa0209970:CDS	45.0%
	GTCAGCATGAGATTGCTTCA+CGG	-	chr2_1:49646798-49646817	None:intergenic	45.0%
!	CCTCTTCTTTTGGCCTATCA+GGG	-	chr2_1:49646853-49646872	None:intergenic	45.0%
!	TCCTCTTCTTTTGGCCTATC+AGG	-	chr2_1:49646854-49646873	None:intergenic	45.0%
	TGTGGTAGAAAGCCAACTAG+AGG	-	chr2_1:49646987-49647006	None:intergenic	45.0%
	TGACTGCTAGAAGAAGGGTT+GGG	+	chr2_1:49647071-49647090	Msa0209970:three_prime_UTR	45.0%
	AGAAGTTACCCTGCCATTAC+AGG	+	chr2_1:49647195-49647214	Msa0209970:three_prime_UTR	45.0%
	GAAGTTACCCTGCCATTACA+GGG	+	chr2_1:49647196-49647215	Msa0209970:three_prime_UTR	45.0%
	TCAAACTGCATTGTACTCGG+AGG	+	chr2_1:49647235-49647254	Msa0209970:three_prime_UTR	45.0%
	TTCTTGGAACATGCAGTTCG+TGG	+	chr2_1:49647274-49647293	Msa0209970:three_prime_UTR	45.0%
	TACTCCTCAGATTGAGGAGT+TGG	+	chr2_1:49645576-49645595	Msa0209970:CDS	45.0%
	ACTCCTCAGATTGAGGAGTT+GGG	+	chr2_1:49645577-49645596	Msa0209970:CDS	45.0%
	GAGGTATGAGATGGACATGT+TGG	+	chr2_1:49645654-49645673	Msa0209970:CDS	45.0%
	TTCGTCACTGTTGCAACCTT+GGG	-	chr2_1:49645695-49645714	None:intergenic	45.0%
	GCAGATATGCTTATCCTTGG+TGG	+	chr2_1:49645839-49645858	Msa0209970:intron	45.0%
	GCAGCTGGTGAAATATATGG+TGG	+	chr2_1:49645982-49646001	Msa0209970:CDS	45.0%
	CTTGAGGCATAAGGAAACCT+AGG	+	chr2_1:49646056-49646075	Msa0209970:intron	45.0%
	GGTCAGAAAGGAAACACTGT+TGG	+	chr2_1:49646143-49646162	Msa0209970:intron	45.0%
	TTCCTCTCAAATGGCAGCAT+TGG	+	chr2_1:49646197-49646216	Msa0209970:CDS	45.0%
!	ATCCAATGCTGCCATTTGAG+AGG	-	chr2_1:49646202-49646221	None:intergenic	45.0%
	AGGTCATCGCAGGTAAAGTT+CGG	+	chr2_1:49646290-49646309	Msa0209970:intron	45.0%
	TAACAAGAGCAAGAGGGAGT+AGG	-	chr2_1:49646361-49646380	None:intergenic	45.0%
	GTCAGGTCATTGAGCAAAAG+TGG	-	chr2_1:49646518-49646537	None:intergenic	45.0%
	AGGGTAAAGGTCATGTCACT+TGG	-	chr2_1:49646657-49646676	None:intergenic	45.0%
	GATATCTGCAGGGAATTGGA+GGG	-	chr2_1:49646676-49646695	None:intergenic	45.0%
	AGATATCTGCAGGGAATTGG+AGG	-	chr2_1:49646677-49646696	None:intergenic	45.0%
	CCAAGATATCTGCAGGGAAT+TGG	-	chr2_1:49646680-49646699	None:intergenic	45.0%
	CCAATTCCCTGCAGATATCT+TGG	+	chr2_1:49646677-49646696	Msa0209970:intron	45.0%
	CTTGAACCCAAGATATCTGC+AGG	-	chr2_1:49646687-49646706	None:intergenic	45.0%
!!	ACACAAGTGGAGCATTGAAC+TGG	+	chr2_1:49646756-49646775	Msa0209970:CDS	45.0%
!!	CACAAGTGGAGCATTGAACT+GGG	+	chr2_1:49646757-49646776	Msa0209970:CDS	45.0%
	GTCAGCATGAGATTGCTTCA+CGG	-	chr2_1:49646798-49646817	None:intergenic	45.0%
!	CCTCTTCTTTTGGCCTATCA+GGG	-	chr2_1:49646853-49646872	None:intergenic	45.0%
!	TCCTCTTCTTTTGGCCTATC+AGG	-	chr2_1:49646854-49646873	None:intergenic	45.0%
	TGTGGTAGAAAGCCAACTAG+AGG	-	chr2_1:49646987-49647006	None:intergenic	45.0%
	TGACTGCTAGAAGAAGGGTT+GGG	+	chr2_1:49647071-49647090	Msa0209970:three_prime_UTR	45.0%
	AGAAGTTACCCTGCCATTAC+AGG	+	chr2_1:49647195-49647214	Msa0209970:three_prime_UTR	45.0%
	GAAGTTACCCTGCCATTACA+GGG	+	chr2_1:49647196-49647215	Msa0209970:three_prime_UTR	45.0%
	TCAAACTGCATTGTACTCGG+AGG	+	chr2_1:49647235-49647254	Msa0209970:three_prime_UTR	45.0%
	TTCTTGGAACATGCAGTTCG+TGG	+	chr2_1:49647274-49647293	Msa0209970:three_prime_UTR	45.0%
!!	TATTTATATATCATATATTA+GGG	-	chr2_1:49647340-49647359	None:intergenic	5.0%
!!	ATATTTATATATCATATATT+AGG	-	chr2_1:49647341-49647360	None:intergenic	5.0%
!!	TGATATATAAATATATTATA+AGG	+	chr2_1:49647347-49647366	Msa0209970:three_prime_UTR	5.0%
!!	TATTTATATATCATATATTA+GGG	-	chr2_1:49647340-49647359	None:intergenic	5.0%
!!	ATATTTATATATCATATATT+AGG	-	chr2_1:49647341-49647360	None:intergenic	5.0%
!!	TGATATATAAATATATTATA+AGG	+	chr2_1:49647347-49647366	Msa0209970:three_prime_UTR	5.0%
	AAATCGAACTGATGCTCGGC+TGG	+	chr2_1:49645486-49645505	Msa0209970:CDS	50.0%
!	ATGCTCGGCTGGCCAATAAT+GGG	+	chr2_1:49645497-49645516	Msa0209970:CDS	50.0%
	GATTGAGGAGTTGGGCAGAA+AGG	+	chr2_1:49645585-49645604	Msa0209970:CDS	50.0%
!	GAGGCAGAATGGCCCATTTT+TGG	+	chr2_1:49645621-49645640	Msa0209970:CDS	50.0%
	CTTCGTCACTGTTGCAACCT+TGG	-	chr2_1:49645696-49645715	None:intergenic	50.0%
	GCAGTCTCCTCAGGTGTTAA+CGG	-	chr2_1:49645913-49645932	None:intergenic	50.0%
	GCGATGACCTTCGACTACTT+TGG	-	chr2_1:49646280-49646299	None:intergenic	50.0%
	AAGTAGTCGAAGGTCATCGC+AGG	+	chr2_1:49646280-49646299	Msa0209970:CDS	50.0%
	GGACGGCAAATCATGTAGAG+CGG	-	chr2_1:49646340-49646359	None:intergenic	50.0%
	AAGAGCAAGAGGGAGTAGGA+CGG	-	chr2_1:49646357-49646376	None:intergenic	50.0%
	TGCAGGGAATTGGAGGGTAA+AGG	-	chr2_1:49646670-49646689	None:intergenic	50.0%
	CAATCTCATGCTGACCGCAT+GGG	+	chr2_1:49646803-49646822	Msa0209970:CDS	50.0%
	CCCTGATAGGCCAAAAGAAG+AGG	+	chr2_1:49646850-49646869	Msa0209970:CDS	50.0%
	TCGTACTCGTGTCCTCTAGT+TGG	+	chr2_1:49646972-49646991	Msa0209970:three_prime_UTR	50.0%
	GTGACTGCTAGAAGAAGGGT+TGG	+	chr2_1:49647070-49647089	Msa0209970:three_prime_UTR	50.0%
	GACTGCTAGAAGAAGGGTTG+GGG	+	chr2_1:49647072-49647091	Msa0209970:three_prime_UTR	50.0%
	ACTGCTAGAAGAAGGGTTGG+GGG	+	chr2_1:49647073-49647092	Msa0209970:three_prime_UTR	50.0%
	GATCTGCTCTCACCCTGTAA+TGG	-	chr2_1:49647211-49647230	None:intergenic	50.0%
	AAATCGAACTGATGCTCGGC+TGG	+	chr2_1:49645486-49645505	Msa0209970:CDS	50.0%
!	ATGCTCGGCTGGCCAATAAT+GGG	+	chr2_1:49645497-49645516	Msa0209970:CDS	50.0%
	GATTGAGGAGTTGGGCAGAA+AGG	+	chr2_1:49645585-49645604	Msa0209970:CDS	50.0%
!	GAGGCAGAATGGCCCATTTT+TGG	+	chr2_1:49645621-49645640	Msa0209970:CDS	50.0%
	CTTCGTCACTGTTGCAACCT+TGG	-	chr2_1:49645696-49645715	None:intergenic	50.0%
	GCAGTCTCCTCAGGTGTTAA+CGG	-	chr2_1:49645913-49645932	None:intergenic	50.0%
	GCGATGACCTTCGACTACTT+TGG	-	chr2_1:49646280-49646299	None:intergenic	50.0%
	AAGTAGTCGAAGGTCATCGC+AGG	+	chr2_1:49646280-49646299	Msa0209970:CDS	50.0%
	GGACGGCAAATCATGTAGAG+CGG	-	chr2_1:49646340-49646359	None:intergenic	50.0%
	AAGAGCAAGAGGGAGTAGGA+CGG	-	chr2_1:49646357-49646376	None:intergenic	50.0%
	TGCAGGGAATTGGAGGGTAA+AGG	-	chr2_1:49646670-49646689	None:intergenic	50.0%
	CAATCTCATGCTGACCGCAT+GGG	+	chr2_1:49646803-49646822	Msa0209970:CDS	50.0%
	CCCTGATAGGCCAAAAGAAG+AGG	+	chr2_1:49646850-49646869	Msa0209970:CDS	50.0%
	TCGTACTCGTGTCCTCTAGT+TGG	+	chr2_1:49646972-49646991	Msa0209970:three_prime_UTR	50.0%
	GTGACTGCTAGAAGAAGGGT+TGG	+	chr2_1:49647070-49647089	Msa0209970:three_prime_UTR	50.0%
	GACTGCTAGAAGAAGGGTTG+GGG	+	chr2_1:49647072-49647091	Msa0209970:three_prime_UTR	50.0%
	ACTGCTAGAAGAAGGGTTGG+GGG	+	chr2_1:49647073-49647092	Msa0209970:three_prime_UTR	50.0%
	GATCTGCTCTCACCCTGTAA+TGG	-	chr2_1:49647211-49647230	None:intergenic	50.0%
!	GATGCTCGGCTGGCCAATAA+TGG	+	chr2_1:49645496-49645515	Msa0209970:CDS	55.0%
	CTGCCCAACTCCTCAATCTG+AGG	-	chr2_1:49645583-49645602	None:intergenic	55.0%
!!	GCATTTTCGGGCTGTACCCA+AGG	+	chr2_1:49645676-49645695	Msa0209970:CDS	55.0%
	GTCAAAGGCCCTAAGCGCAA+GGG	-	chr2_1:49645935-49645954	None:intergenic	55.0%
	TGTCAAAGGCCCTAAGCGCA+AGG	-	chr2_1:49645936-49645955	None:intergenic	55.0%
	GCAATCTCATGCTGACCGCA+TGG	+	chr2_1:49646802-49646821	Msa0209970:CDS	55.0%
	CTGCTAGAAGAAGGGTTGGG+GGG	+	chr2_1:49647074-49647093	Msa0209970:three_prime_UTR	55.0%
	GCTCTCACCCTGTAATGGCA+GGG	-	chr2_1:49647206-49647225	None:intergenic	55.0%
	TGCTCTCACCCTGTAATGGC+AGG	-	chr2_1:49647207-49647226	None:intergenic	55.0%
!	GATGCTCGGCTGGCCAATAA+TGG	+	chr2_1:49645496-49645515	Msa0209970:CDS	55.0%
	CTGCCCAACTCCTCAATCTG+AGG	-	chr2_1:49645583-49645602	None:intergenic	55.0%
!!	GCATTTTCGGGCTGTACCCA+AGG	+	chr2_1:49645676-49645695	Msa0209970:CDS	55.0%
	GTCAAAGGCCCTAAGCGCAA+GGG	-	chr2_1:49645935-49645954	None:intergenic	55.0%
	TGTCAAAGGCCCTAAGCGCA+AGG	-	chr2_1:49645936-49645955	None:intergenic	55.0%
	GCAATCTCATGCTGACCGCA+TGG	+	chr2_1:49646802-49646821	Msa0209970:CDS	55.0%
	CTGCTAGAAGAAGGGTTGGG+GGG	+	chr2_1:49647074-49647093	Msa0209970:three_prime_UTR	55.0%
	GCTCTCACCCTGTAATGGCA+GGG	-	chr2_1:49647206-49647225	None:intergenic	55.0%
	TGCTCTCACCCTGTAATGGC+AGG	-	chr2_1:49647207-49647226	None:intergenic	55.0%
	AGGAGACTGCCCTTGCGCTT+AGG	+	chr2_1:49645923-49645942	Msa0209970:CDS	60.0%
	GGAGACTGCCCTTGCGCTTA+GGG	+	chr2_1:49645924-49645943	Msa0209970:CDS	60.0%
	TCTCATGCTGACCGCATGGG+CGG	+	chr2_1:49646806-49646825	Msa0209970:CDS	60.0%
!	TTTTCGTTGCGCCGCCCATG+CGG	-	chr2_1:49646820-49646839	None:intergenic	60.0%
	GGGCGGCGCAACGAAAAGAT+TGG	+	chr2_1:49646823-49646842	Msa0209970:CDS	60.0%
	AGGAGACTGCCCTTGCGCTT+AGG	+	chr2_1:49645923-49645942	Msa0209970:CDS	60.0%
	GGAGACTGCCCTTGCGCTTA+GGG	+	chr2_1:49645924-49645943	Msa0209970:CDS	60.0%
	TCTCATGCTGACCGCATGGG+CGG	+	chr2_1:49646806-49646825	Msa0209970:CDS	60.0%
!	TTTTCGTTGCGCCGCCCATG+CGG	-	chr2_1:49646820-49646839	None:intergenic	60.0%
	GGGCGGCGCAACGAAAAGAT+TGG	+	chr2_1:49646823-49646842	Msa0209970:CDS	60.0%
	AGCGCAAGGGCAGTCTCCTC+AGG	-	chr2_1:49645922-49645941	None:intergenic	65.0%
	AGCGCAAGGGCAGTCTCCTC+AGG	-	chr2_1:49645922-49645941	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	49645457	49647487	49645457	ID=Msa0209970;Name=Msa0209970
chr2_1	mRNA	49645457	49647487	49645457	ID=Msa0209970-mRNA-1;Parent=Msa0209970;Name=Msa0209970-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0\|1\|0.75\|1\|1\|1\|4\|541\|299
chr2_1	exon	49645457	49645737	49645457	ID=Msa0209970-mRNA-1:exon:13728;Parent=Msa0209970-mRNA-1
chr2_1	exon	49645843	49646047	49645843	ID=Msa0209970-mRNA-1:exon:13729;Parent=Msa0209970-mRNA-1
chr2_1	exon	49646144	49646301	49646144	ID=Msa0209970-mRNA-1:exon:13730;Parent=Msa0209970-mRNA-1
chr2_1	exon	49646691	49647487	49646691	ID=Msa0209970-mRNA-1:exon:13731;Parent=Msa0209970-mRNA-1
chr2_1	CDS	49645457	49645737	49645457	ID=Msa0209970-mRNA-1:cds;Parent=Msa0209970-mRNA-1
chr2_1	CDS	49645843	49646047	49645843	ID=Msa0209970-mRNA-1:cds;Parent=Msa0209970-mRNA-1
chr2_1	CDS	49646144	49646301	49646144	ID=Msa0209970-mRNA-1:cds;Parent=Msa0209970-mRNA-1
chr2_1	CDS	49646691	49646946	49646691	ID=Msa0209970-mRNA-1:cds;Parent=Msa0209970-mRNA-1
chr2_1	three_prime_UTR	49646947	49647487	49646947	ID=Msa0209970-mRNA-1:three_prime_utr;Parent=Msa0209970-mRNA-1

Gene Sequence

>Msa0209970

TTGATACAAAAGTATAAAGTTGTCCATTTAAATCGAACTGATGCTCGGCTGGCCAATAATGGGCAATCTTTAGAGATTCAGAAGCTGCGTTGCCGAGTCAATTTTAGTGCTCTTAGATTTACTCCTCAGATTGAGGAGTTGGGCAGAAAGGTTATCAATCTTCTGAGGCAGAATGGCCCATTTTTGGTACTTCATCTGAGGTATGAGATGGACATGTTGGCATTTTCGGGCTGTACCCAAGGTTGCAACAGTGACGAAGTTGAAGAATTGACAAGGATGAGATATGCTTATCCTTGGTGGAAAGAAAAAATAATTAATTCTGATTTGAAAAGAAAAGATGGTTTATGTCCGTTAACACCTGAGGAGACTGCCCTTGCGCTTAGGGCCTTTGACATTGATCAAAACATTCAAATCTACATTGCAGCTGGTGAAATATATGGTGGGAGTAGAAGAATGGCCAGTCTAGCAAAGAACTATCCAAAATTGGTCAGAAAGGAAACACTGTTGGAACCGTCTGAACTTCAGTTCTTCCAAAATCATTCCTCTCAAATGGCAGCATTGGATTATCTTGTCTCATTAGAGAGTGATATCTTTGTTCCTACATACGATGGAAATATGGCCAAAGTAGTCGAAGGTCATCGCAGATATCTTGGGTTCAAGAAGACCATTTTATTGAACAGAAAGCTGCTAGTTGAATTGATAGATCAATACACAAGTGGAGCATTGAACTGGGAAGAATTCTCTTCGGCCGTGAAGCAATCTCATGCTGACCGCATGGGCGGCGCAACGAAAAGATTGGTAATCCCTGATAGGCCAAAAGAAGAGGATTATTTCTATGCCAATCCAGATGAATGTTTGGAACAATCAGAGGATGATGATATGTTGAGTAGTACAGCTTAA

Protein sequence

>Msa0209970

LIQKYKVVHLNRTDARLANNGQSLEIQKLRCRVNFSALRFTPQIEELGRKVINLLRQNGPFLVLHLRYEMDMLAFSGCTQGCNSDEVEELTRMRYAYPWWKEKIINSDLKRKDGLCPLTPEETALALRAFDIDQNIQIYIAAGEIYGGSRRMASLAKNYPKLVRKETLLEPSELQFFQNHSSQMAALDYLVSLESDIFVPTYDGNMAKVVEGHRRYLGFKKTILLNRKLLVELIDQYTSGALNWEEFSSAVKQSHADRMGGATKRLVIPDRPKEEDYFYANPDECLEQSEDDDMLSSTA*