Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0220770 | XP_024632453.1 | 95.726 | 585 | 25 | 0 | 1 | 585 | 108 | 692 | 0.0 | 1125 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0220770 | sp|O49445|LRK72_ARATH | 52.962 | 557 | 249 | 8 | 4 | 556 | 114 | 661 | 0.0 | 558 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0220770 | A0A396JHB5 | 95.726 | 585 | 25 | 0 | 1 | 585 | 108 | 692 | 0.0 | 1125 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0020410 | Msa0220770 | 0.814478 | 1.509643e-51 | -8.615850e-47 |
| Msa0150230 | Msa0220770 | 0.801530 | 8.621613e-49 | -8.615850e-47 |
| Msa0220770 | Msa0241140 | 0.811336 | 7.368362e-51 | -8.615850e-47 |
| Msa0220770 | Msa0299930 | 0.849650 | 2.822547e-60 | -8.615850e-47 |
| Msa0220770 | Msa0305410 | 0.811036 | 8.559687e-51 | -8.615850e-47 |
| Msa0220770 | Msa0822500 | 0.801817 | 7.526770e-49 | -8.615850e-47 |
| Msa0220770 | Msa0894710 | 0.804455 | 2.142366e-49 | -8.615850e-47 |
| Msa0220770 | Msa1291450 | 0.805250 | 1.461281e-49 | -8.615850e-47 |
| Msa0220770 | Msa1401990 | 0.806568 | 7.723514e-50 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0220770 | MtrunA17_Chr2g0324481 | 95.726 | 585 | 25 | 0 | 1 | 585 | 108 | 692 | 0.0 | 1125 |
| Msa0220770 | MtrunA17_Chr2g0324171 | 54.252 | 588 | 253 | 9 | 3 | 585 | 113 | 689 | 0.0 | 589 |
| Msa0220770 | MtrunA17_Chr2g0324471 | 55.752 | 565 | 237 | 10 | 3 | 563 | 133 | 688 | 0.0 | 588 |
| Msa0220770 | MtrunA17_Chr2g0282271 | 54.457 | 516 | 227 | 4 | 3 | 513 | 112 | 624 | 0.0 | 548 |
| Msa0220770 | MtrunA17_Chr4g0058561 | 45.124 | 523 | 268 | 10 | 5 | 519 | 122 | 633 | 8.55e-140 | 421 |
| Msa0220770 | MtrunA17_Chr7g0239371 | 43.689 | 515 | 266 | 12 | 6 | 513 | 114 | 611 | 3.98e-137 | 414 |
| Msa0220770 | MtrunA17_Chr7g0239381 | 42.969 | 512 | 267 | 12 | 10 | 513 | 121 | 615 | 1.69e-132 | 402 |
| Msa0220770 | MtrunA17_Chr7g0239321 | 43.605 | 516 | 260 | 12 | 9 | 512 | 115 | 611 | 2.05e-130 | 396 |
| Msa0220770 | MtrunA17_Chr7g0239211 | 42.105 | 513 | 273 | 11 | 10 | 512 | 116 | 614 | 5.31e-126 | 385 |
| Msa0220770 | MtrunA17_Chr7g0239361 | 42.442 | 516 | 270 | 9 | 10 | 512 | 130 | 631 | 5.33e-124 | 380 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0220770 | AT4G28350.1 | 52.962 | 557 | 249 | 8 | 4 | 556 | 114 | 661 | 0.0 | 558 |
| Msa0220770 | AT4G04960.1 | 54.633 | 518 | 229 | 4 | 3 | 516 | 110 | 625 | 0.0 | 533 |
| Msa0220770 | AT4G04960.2 | 55.484 | 465 | 201 | 4 | 3 | 463 | 110 | 572 | 9.21e-167 | 487 |
| Msa0220770 | AT3G55550.1 | 44.444 | 522 | 261 | 12 | 3 | 512 | 114 | 618 | 4.31e-137 | 414 |
| Msa0220770 | AT3G53810.1 | 44.316 | 519 | 263 | 12 | 6 | 513 | 118 | 621 | 1.71e-134 | 407 |
| Msa0220770 | AT4G02410.1 | 42.857 | 525 | 275 | 12 | 2 | 513 | 116 | 628 | 7.69e-134 | 406 |
| Msa0220770 | AT2G37710.1 | 44.639 | 513 | 261 | 12 | 10 | 513 | 122 | 620 | 5.81e-131 | 399 |
| Msa0220770 | AT5G59260.1 | 42.264 | 530 | 266 | 12 | 4 | 513 | 117 | 626 | 3.08e-130 | 397 |
| Msa0220770 | AT4G02420.1 | 42.218 | 514 | 276 | 10 | 10 | 513 | 121 | 623 | 9.55e-129 | 392 |
| Msa0220770 | AT5G59270.2 | 41.431 | 531 | 274 | 11 | 8 | 513 | 121 | 639 | 9.06e-125 | 383 |
Find 96 sgRNAs with CRISPR-Local
Find 276 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGGAGGGAAATTGGTAC+TGG | 0.487875 | 2_1:-69208186 | Msa0220770:CDS |
| AAAGATATGAATGCAAGAAT+AGG | 0.487869 | 2_1:-69207975 | Msa0220770:CDS |
| AAAGGTTGGAGTAAAGAAAA+AGG | 0.261402 | 2_1:-69208203 | Msa0220770:CDS |
| AAATAAACTTAGCAGCATCT+TGG | 0.491818 | 2_1:-69207509 | Msa0220770:CDS |
| AAATGTGTCTTATGCCAGCA+TGG | 0.600245 | 2_1:-69207400 | Msa0220770:CDS |
| AAATTCATATACAAATCTAA+GGG | 0.601303 | 2_1:-69208581 | Msa0220770:CDS |
| AAGAGAAAGAATTTGAAGAT+TGG | 0.346630 | 2_1:-69208466 | Msa0220770:CDS |
| AAGAGACCAAGTTGCTTAAT+TGG | 0.191957 | 2_1:-69208112 | Msa0220770:CDS |
| AAGATTGGGAATTGGAGTAT+TGG | 0.479298 | 2_1:-69208451 | Msa0220770:CDS |
| AATACCAAATCAACCAATGG+TGG | 0.631455 | 2_1:+69207762 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GTGGTGAAAATTATCAAGTT+TGG | - | chr2_1:69207609-69207628 | Msa0220770:CDS | 30.0% | |
| AATTAATGTTACTATGGCTC+CGG | - | chr2_1:69207652-69207671 | Msa0220770:CDS | 30.0% | |
| TTACTAATCAAAGGCCTATT+AGG | + | chr2_1:69207692-69207711 | None:intergenic | 30.0% | |
| AGAATTTGAAGATTGGGAAT+TGG | - | chr2_1:69208006-69208025 | Msa0220770:CDS | 30.0% | |
| AAAGGTTGGAGTAAAGAAAA+AGG | - | chr2_1:69208262-69208281 | Msa0220770:CDS | 30.0% | |
| TAAAGAAAAAGGAGGGAAAT+TGG | - | chr2_1:69208273-69208292 | Msa0220770:CDS | 30.0% | |
| GAAAATACCAAATCAACCAA+TGG | + | chr2_1:69208709-69208728 | None:intergenic | 30.0% | |
| AAATAAACTTAGCAGCATCT+TGG | - | chr2_1:69208956-69208975 | Msa0220770:CDS | 30.0% | |
| GTGGTGAAAATTATCAAGTT+TGG | - | chr2_1:69207609-69207628 | Msa0220770:CDS | 30.0% | |
| AATTAATGTTACTATGGCTC+CGG | - | chr2_1:69207652-69207671 | Msa0220770:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_1 | gene | 69207365 | 69209122 | 69207365 | ID=Msa0220770;Name=Msa0220770 |
| chr2_1 | mRNA | 69207365 | 69209122 | 69207365 | ID=Msa0220770-mRNA-1;Parent=Msa0220770;Name=Msa0220770-mRNA-1;_AED=0.29;_eAED=0.29;_QI=0|-1|0|1|-1|1|1|0|585 |
| chr2_1 | exon | 69207365 | 69209122 | 69207365 | ID=Msa0220770-mRNA-1:exon:19625;Parent=Msa0220770-mRNA-1 |
| chr2_1 | CDS | 69207365 | 69209122 | 69207365 | ID=Msa0220770-mRNA-1:cds;Parent=Msa0220770-mRNA-1 |
| Gene Sequence |
>Msa0220770 ATGGTGATCAATGGTGCACAATCAGGAAACTATCTTGGACTGTTTAATAGAACCTCATCAGGTAATGATTATAATCATGTCTTTGCAGTTGAGTTTGATGATTTTAGAAATGAAGAGTTTGGTGAAGTTAATGATAATCATGTTGGTGTTGATTTGAATTCTATTATTTCTAATTATTCTGAACCTGCTGAGTTTTGGGGTGGTGGAAAAAATGGTGATGAATTGGAAAAGTTGAAACTGTCTAGTGGTGAAAATTATCAAGTTTGGATTGATTTTAGGGAGTTTGAAATTAATGTTACTATGGCTCCGGCGGGGAAAAGAAAACCTAATAGGCCTTTGATTAGTAAGCCTATTAATTTTTCTAGGGTTTTTTTGGATGAAATGTATGTTGGATTTTCGGGTTCGACTGGGAGAATGGTTGATACTTGTAGGATTTTGGCTTGGAGTTTTAGTCATTCGAATTTTTCGATTGGTGATGCTTTGAATACTAAGAATTTACCTGTTTATGTGAATTCAAAGAAATTCATATACAAATCTAAGGGTTTTATTGTAAGTGTGATTTTTGGTTCGGTTTTAGTCTTTGGTTGTTGCACTTTGGTTTCATTCATTTTGTTGAGAAAAAAGAAGGAAGAGAAAGAGAAAGAATTTGAAGATTGGGAATTGGAGTATTGGCCACATAAGATTAGCTATAAAGAGATTTGTGATGCAACAAGTGGATTTTCTGAAGAGAATGTGATAGGGATTGGAACAAGTGGGAAAGTATACAAAGGAGTTTTGAAAGGAGGAGACGAAGTTGCGGTGAAGAGATTCAATCATGATACTCAGCATGGAATGAGAGGCTTTCTAGCTGAGATTTCAAGCTTAGGAAGACTGAAACACAGAAACTTGTTGGGTTTCAAAGGTTGGAGTAAAGAAAAAGGAGGGAAATTGGTACTGGTTTATGATTACATGGAGAATGAGAGTTTAGATAAAAGAATCTTTGAGTGTGAAGAGACCAAGTTGCTTAATTGGGAAGAAAGAATAAGAGTCCTAGAAAATGTAGCTAGTGGAATTTTATATCTACATGAAGGTTGGGATTTTGAGGTTTTGCATAGAGATATTAAAGCAAGTAATGTACTACTTGACAAAGATATGAATGCAAGAATAGGTGATTTTGGTCTTGCTAGGTTGCGGCATCAAGAACAAGTAGCTGACACAACAAGTGTGATAGGTACTTTAGGGTACATGGCACCAGAACTCGTTAAAGTCGGTAAACCATCAACTGCAACTGATGTTTATAGTTTTGGAATAGTGGTTTTAGAAGTTGTATGTGGTAGAAGGCCTATTGTAACGGATGAACCACCATTGGTTGATTTGGTATTTTCTCTTATGGAGAAAGGTGAATTGAGTAGCGCAATTGATGAACGTTTGAAGGGTCAAATGGAGTGTAAATACAACACTGTGGTAGCTGAGAGATTGCTTCGTTTGGGTTTGTTATGTGCTAGTGTAGACCCTGGTGTTCGACCAACAATGAGACAGGTTGTGAAAGCTTTGGAGGGTGTAAAATGCACTGAGTGTAATGAAGAGTGTGTTCATGTGGGTCTTATTGGAAAAATAAACTTAGCAGCATCTTGGTCTAAGAATTCTTATGATTCGGCACGTTTAAACTCTCCTACCCTTGCTGATATTTTGCCATTCAAATATTTTTCTGGAGATTGTTTAAATGTGTCTTATGCCAGCATGGAGCCATTGTCTGATTCTCTCTCAGAAGGAAGGTGA |
| Protein sequence |
>Msa0220770 MVINGAQSGNYLGLFNRTSSGNDYNHVFAVEFDDFRNEEFGEVNDNHVGVDLNSIISNYSEPAEFWGGGKNGDELEKLKLSSGENYQVWIDFREFEINVTMAPAGKRKPNRPLISKPINFSRVFLDEMYVGFSGSTGRMVDTCRILAWSFSHSNFSIGDALNTKNLPVYVNSKKFIYKSKGFIVSVIFGSVLVFGCCTLVSFILLRKKKEEKEKEFEDWELEYWPHKISYKEICDATSGFSEENVIGIGTSGKVYKGVLKGGDEVAVKRFNHDTQHGMRGFLAEISSLGRLKHRNLLGFKGWSKEKGGKLVLVYDYMENESLDKRIFECEETKLLNWEERIRVLENVASGILYLHEGWDFEVLHRDIKASNVLLDKDMNARIGDFGLARLRHQEQVADTTSVIGTLGYMAPELVKVGKPSTATDVYSFGIVVLEVVCGRRPIVTDEPPLVDLVFSLMEKGELSSAIDERLKGQMECKYNTVVAERLLRLGLLCASVDPGVRPTMRQVVKALEGVKCTECNEECVHVGLIGKINLAASWSKNSYDSARLNSPTLADILPFKYFSGDCLNVSYASMEPLSDSLSEGR* |
