AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0225580

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0225580 MtrunA17_Chr2g0331821 93.718 780 22 8 1 773 1 760 0.0 1373
Msa0225580 MtrunA17_Chr3g0139411 55.678 546 233 5 220 765 168 704 0.0 590
Msa0225580 MtrunA17_Chr3g0139411 66.667 60 20 0 1 60 1 60 5.19e-14 75.9
Msa0225580 MtrunA17_Chr4g0004471 58.000 450 178 5 327 773 117 558 1.09e-172 508
Msa0225580 MtrunA17_Chr4g0004471 67.797 59 19 0 1 59 1 59 2.56e-13 73.6
Msa0225580 MtrunA17_Chr5g0405741 31.347 453 270 9 326 758 197 628 6.39e-54 197
Msa0225580 MtrunA17_Chr5g0405741 49.153 59 30 0 1 59 1 59 7.08e-12 68.9
Msa0225580 MtrunA17_Chr8g0335341 29.864 442 283 10 327 751 274 705 1.11e-50 189
Msa0225580 MtrunA17_Chr8g0335341 57.627 59 25 0 1 59 1 59 2.46e-14 77.0
Msa0225580 MtrunA17_Chr7g0250681 29.252 441 291 7 321 751 396 825 7.58e-49 185
Msa0225580 MtrunA17_Chr7g0250681 57.627 59 25 0 1 59 1 59 9.39e-14 75.5
Msa0225580 MtrunA17_Chr1g0211271 27.790 457 297 10 335 766 276 724 4.93e-44 170
Msa0225580 MtrunA17_Chr2g0303521 28.504 421 280 7 338 751 213 619 1.42e-39 155
Msa0225580 MtrunA17_Chr4g0072011 29.091 330 211 6 334 646 336 659 2.83e-36 146
Msa0225580 MtrunA17_Chr4g0072011 54.545 55 25 0 1 55 1 55 3.71e-11 66.6
Msa0225580 MtrunA17_Chr5g0448571 29.003 331 224 6 346 671 367 691 3.38e-33 137
Msa0225580 MtrunA17_Chr3g0124841 22.926 458 310 7 334 761 455 899 5.36e-29 124
Msa0225580 MtrunA17_Chr2g0298171 27.826 345 210 11 333 646 532 868 1.69e-28 122
Msa0225580 MtrunA17_Chr2g0298171 50.847 59 29 0 1 59 1 59 5.39e-12 69.7
Msa0225580 MtrunA17_Chr3g0118401 25.495 404 232 11 299 647 291 680 3.08e-28 121
Msa0225580 MtrunA17_Chr3g0130761 22.588 456 307 7 332 761 339 774 4.26e-27 117
Msa0225580 MtrunA17_Chr3g0143731 25.826 333 224 6 319 643 269 586 1.06e-26 116
Msa0225580 MtrunA17_Chr5g0440551 26.683 401 232 12 290 637 263 654 3.76e-26 114
Msa0225580 MtrunA17_Chr8g0382541 30.864 162 110 2 494 654 1 161 5.71e-16 78.2
Msa0225580 MtrunA17_Chr8g0382551 27.485 171 86 5 334 468 269 437 1.01e-11 67.8
Msa0225580 MtrunA17_Chr1g0208081 38.356 73 45 0 414 486 5 77 4.05e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0225580 AT1G52320.2 49.465 841 300 15 1 771 1 786 0.0 693
Msa0225580 AT1G52320.5 49.465 841 300 15 1 771 1 786 0.0 693
Msa0225580 AT1G52320.1 48.778 818 294 15 24 771 1 763 0.0 654
Msa0225580 AT1G52320.3 64.454 467 151 2 305 771 9 460 0.0 628
Msa0225580 AT1G52320.4 64.454 467 151 2 305 771 9 460 0.0 628
Msa0225580 AT5G25590.1 60.730 466 179 2 310 771 303 768 0.0 553
Msa0225580 AT5G25590.1 62.712 59 22 0 1 59 1 59 9.35e-18 88.6
Msa0225580 AT3G51290.3 31.696 448 269 8 326 758 193 618 4.40e-57 206
Msa0225580 AT3G51290.2 32.054 443 274 7 326 758 193 618 8.89e-57 208
Msa0225580 AT1G02110.1 29.140 477 314 9 285 751 223 685 3.23e-54 199
Msa0225580 AT1G02110.1 54.237 59 27 0 1 59 1 59 5.14e-13 73.2
Msa0225580 AT3G51290.1 31.250 448 265 9 326 758 193 612 3.03e-52 192
Msa0225580 AT3G60320.1 29.933 451 286 11 324 760 349 783 9.98e-45 173
Msa0225580 AT4G39790.1 30.725 345 192 8 334 633 252 594 1.28e-35 144
Msa0225580 AT1G21740.1 24.571 525 334 11 263 758 447 938 3.40e-35 144
Msa0225580 AT2G34670.2 28.806 427 267 9 338 747 254 660 3.74e-34 140
Msa0225580 AT4G35240.2 27.492 331 233 4 337 661 423 752 6.21e-34 140
Msa0225580 AT4G35240.1 27.492 331 233 4 337 661 423 752 6.21e-34 140
Msa0225580 AT4G35240.4 27.492 331 233 4 337 661 423 752 6.79e-34 140
Msa0225580 AT4G35240.3 27.492 331 233 4 337 661 423 752 6.79e-34 140
Msa0225580 AT1G77500.1 26.126 444 283 9 334 758 450 867 5.71e-33 137
Msa0225580 AT2G27090.2 28.255 361 213 8 346 664 320 676 3.09e-32 134
Msa0225580 AT2G27090.1 28.255 361 213 8 346 664 320 676 3.09e-32 134
Msa0225580 AT2G17110.1 27.019 322 221 3 337 645 313 633 1.38e-30 129
Msa0225580 AT1G20530.1 27.171 357 238 6 332 682 230 570 3.64e-28 120
Msa0225580 AT2G19090.1 28.448 348 204 7 333 646 385 721 3.57e-26 115
Msa0225580 AT4G30130.3 30.645 248 156 3 414 649 398 641 8.54e-25 110
Msa0225580 AT4G30130.1 30.645 248 156 3 414 649 398 641 8.54e-25 110
Msa0225580 AT2G34670.1 30.000 300 188 6 338 627 254 541 1.86e-22 102
Msa0225580 AT5G54480.1 22.330 412 268 10 355 758 345 712 2.71e-16 84.0
Msa0225580 AT4G30130.4 40.964 83 49 0 414 496 398 480 1.11e-13 75.1
Msa0225580 AT4G30130.2 40.964 83 49 0 414 496 398 480 1.11e-13 75.1

Find 194 sgRNAs with CRISPR-Local

Find 285 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTAAAGGAATACCTAATT+TGG 0.100959 2_1:+75696675 Msa0225580:CDS
ACATAATAATAGTGTTAATT+TGG 0.152686 2_1:+75695929 Msa0225580:CDS
CAAATTAGGTATTCCTTTAA+AGG 0.164407 2_1:-75696674 None:intergenic
CCCTATTGAGAGCAGTTTAA+AGG 0.173187 2_1:+75697921 Msa0225580:CDS
ACTTGGAATCGATCCTTTAA+AGG 0.204615 2_1:+75696661 Msa0225580:CDS
TCTAAAATGTACATGGAATT+AGG 0.206688 2_1:+75698420 Msa0225580:CDS
GTTTCAAGTAATAATAATTT+TGG 0.212878 2_1:-75694841 None:intergenic
GGCTTATTGATCTTGATTTC+AGG 0.226164 2_1:-75695282 None:intergenic
ATCGCATGCCAGAGCTCTTC+AGG 0.228632 2_1:+75698370 Msa0225580:CDS
TTTGGTATGATGGATGATTT+TGG 0.228967 2_1:-75694823 None:intergenic
ACCTCTATTATCAGCAAAAT+TGG 0.239779 2_1:-75696051 None:intergenic
GAAACTCTTGTCTTTGTCTT+TGG 0.248030 2_1:+75694742 Msa0225580:five_prime_UTR
GCCTCTGGATCAACTTCTTC+TGG 0.257510 2_1:-75698204 None:intergenic
TGAGCGAACTTATCAGTTAT+TGG 0.286444 2_1:+75697801 Msa0225580:CDS
GCCGAATTATGTTGCGGATT+CGG 0.297762 2_1:-75695603 None:intergenic
CATTACCATACCTAATCTTC+AGG 0.307320 2_1:-75698587 None:intergenic
GAAAACATGAACAAACAATA+AGG 0.309465 2_1:-75698673 None:intergenic
CACTTGTCTGCTTAGGAGAT+TGG 0.327106 2_1:-75697768 None:intergenic
ACATAAAGTCCCTAAGTAAT+TGG 0.330244 2_1:+75697881 Msa0225580:CDS
GTTGGTGTTGGTGTTGGTGC+TGG 0.334738 2_1:+75695429 Msa0225580:CDS
TCCTTTAAACTGCTCTCAAT+AGG 0.339506 2_1:-75697922 None:intergenic
TCCGAATCCGCAACATAATT+CGG 0.340331 2_1:+75695602 Msa0225580:CDS
TCCTTCCCGTCATCCAAATT+AGG 0.341107 2_1:-75696688 None:intergenic
GGTGTTGGTGTTGAGATTCC+TGG 0.349593 2_1:+75695786 Msa0225580:CDS
GAACTCATCATGAGGTGCTT+TGG 0.357428 2_1:+75698467 Msa0225580:exon
TAGTGAATAGTGAGTGGGTT+TGG 0.362633 2_1:-75694886 None:intergenic
TCTTGAAATCTCTTGATTTG+TGG 0.362920 2_1:+75698558 Msa0225580:three_prime_UTR
AACTCATCATGAGGTGCTTT+GGG 0.366147 2_1:+75698468 Msa0225580:exon
CAATTGACTGACAGCTGCTT+TGG 0.366439 2_1:-75697231 None:intergenic
CCATCAATGGAGGATATTGC+AGG 0.367960 2_1:+75695645 Msa0225580:CDS
ATGGAGTCACCGTTGCCATC+AGG 0.368861 2_1:+75695846 Msa0225580:CDS
TATTGTAGATATGCAATCAT+TGG 0.371247 2_1:+75697265 Msa0225580:CDS
ACAATTGTTCATCGCGTAAT+CGG 0.371613 2_1:-75697310 None:intergenic
GGAAGTGGTGGTGGAGGAAG+AGG 0.375556 2_1:-75695222 None:intergenic
TCCATCGAAGGAGGCGTAAC+CGG 0.384107 2_1:-75695888 None:intergenic
CCTTTAAACTGCTCTCAATA+GGG 0.384711 2_1:-75697921 None:intergenic
GAAACTTTATGATGAAGTTA+AGG 0.388245 2_1:+75696777 Msa0225580:CDS
GTGGATGAGCCCTGAAGATT+AGG 0.388403 2_1:+75698577 Msa0225580:three_prime_UTR
TTGGGCATGGGCCTAGGATC+GGG 0.391219 2_1:-75695303 None:intergenic
AAGCACCTCATGATGAGTTC+TGG 0.393286 2_1:-75698464 None:intergenic
ATGGGATTGGGCATGGGCCT+AGG 0.399611 2_1:-75695309 None:intergenic
TTCTTCCTCTAGAATGGGAT+TGG 0.405015 2_1:-75695322 None:intergenic
CTCTAGAATGGGATTGGGCA+TGG 0.405160 2_1:-75695316 None:intergenic
GTTGGTGTTGGTGCTGGTGG+AGG 0.405505 2_1:+75695435 Msa0225580:CDS
ACACCCATTCGAGCTGTAAC+AGG 0.407056 2_1:-75695768 None:intergenic
ATTGGGCATGGGCCTAGGAT+CGG 0.407697 2_1:-75695304 None:intergenic
TCAACAAGCTTAACAAGTCT+TGG 0.408725 2_1:-75697335 None:intergenic
CCGCAAAACCAGGCAATTTG+AGG 0.409368 2_1:+75698149 Msa0225580:CDS
AATTCAGGAAGTGGTGGTGG+AGG 0.413282 2_1:-75695228 None:intergenic
ATCATCTTCTTCCTCTAGAA+TGG 0.415255 2_1:-75695328 None:intergenic
GAAAAGTTGCCTCACTAAAC+AGG 0.416122 2_1:+75697168 Msa0225580:CDS
GAGAATTCAGGAAGTGGTGG+TGG 0.419059 2_1:-75695231 None:intergenic
CGCAACATAATTCGGCTGCT+TGG 0.420609 2_1:+75695610 Msa0225580:CDS
AGCATACTGCAATGCCTAGT+AGG 0.421867 2_1:+75695727 Msa0225580:CDS
CCTGCAATATCCTCCATTGA+TGG 0.422082 2_1:-75695645 None:intergenic
TGAAGAGTAGGAGAGAATTC+AGG 0.425943 2_1:-75695243 None:intergenic
GAATAGTAATAGAAGATTAG+AGG 0.427514 2_1:+75695461 Msa0225580:CDS
ACTTGTCTGCTTAGGAGATT+GGG 0.429628 2_1:-75697767 None:intergenic
TCTTGGACAAGTTGCTAGCT+TGG 0.429870 2_1:+75696749 Msa0225580:CDS
GCAACATAATTCGGCTGCTT+GGG 0.437189 2_1:+75695611 Msa0225580:CDS
CAAAGATGCTTGAGGCTACA+AGG 0.437960 2_1:+75696015 Msa0225580:CDS
GCTGTCCTAGCAAGCTCATC+GGG 0.447333 2_1:-75698027 None:intergenic
TTGAATTAGTGAATAGTGAG+TGG 0.454162 2_1:-75694892 None:intergenic
CCAATATGATCACTACTAAT+CGG 0.454406 2_1:-75695537 None:intergenic
ATTATGTTGCGGATTCGGAT+TGG 0.454628 2_1:-75695598 None:intergenic
CTTGGACAAGTTGCTAGCTT+GGG 0.454835 2_1:+75696750 Msa0225580:CDS
TTCTCTGTAACAATTTCATC+AGG 0.455151 2_1:-75698243 None:intergenic
GATGCTAGTCAACAATCAAA+AGG 0.457427 2_1:+75697855 Msa0225580:CDS
TCATCTTCTTCCTCTAGAAT+GGG 0.460447 2_1:-75695327 None:intergenic
GGTGTTGGTGTTGGTGCTGG+TGG 0.464159 2_1:+75695432 Msa0225580:CDS
AAAAGCAATGCCTCCAAGTA+AGG 0.465352 2_1:+75698322 Msa0225580:CDS
GTTGGTGTTGGTGTTGGTGT+TGG 0.466726 2_1:+75695423 Msa0225580:CDS
GCATTGGCATCTTCAGCCTC+TGG 0.467662 2_1:-75698219 None:intergenic
AACAATTTCATCAGGAGCAT+TGG 0.468857 2_1:-75698235 None:intergenic
GCCTAGTAGGGTTGGTATTG+TGG 0.472234 2_1:+75695740 Msa0225580:CDS
GAAGGCCAACATGGAGGAGG+AGG 0.473560 2_1:+75695684 Msa0225580:CDS
ATGCCGGTGGTGGTTGATTG+AGG 0.474630 2_1:-75695132 None:intergenic
TAGAAAACGAAGGCTCTTTG+AGG 0.475994 2_1:+75695373 Msa0225580:CDS
TCTAGAATGGGATTGGGCAT+GGG 0.477320 2_1:-75695315 None:intergenic
GTAAATCAAATCAAACTCAA+TGG 0.480453 2_1:+75694927 Msa0225580:exon
TAAATCAAATCAAACTCAAT+GGG 0.485036 2_1:+75694928 Msa0225580:exon
AAAACATGAACAAACAATAA+GGG 0.485706 2_1:-75698672 None:intergenic
TGGTGTTCACTTGTCTGCTT+AGG 0.485850 2_1:-75697775 None:intergenic
GAGCTTGCTAGGACAGCTAT+AGG 0.486641 2_1:+75698033 Msa0225580:CDS
CCGATTAGTAGTGATCATAT+TGG 0.489027 2_1:+75695537 Msa0225580:CDS
CGCTGAGGCTGAGGAAGTGA+TGG 0.491248 2_1:+75695815 Msa0225580:CDS
GCCGGTTACGCCTCCTTCGA+TGG 0.492483 2_1:+75695887 Msa0225580:CDS
GGGTCATGCGAGTTATTACT+TGG 0.495204 2_1:+75696644 Msa0225580:CDS
AAACCAGGCAATTTGAGGAT+TGG 0.497907 2_1:+75698154 Msa0225580:CDS
TGAAACTCATGCTACCATCT+TGG 0.499419 2_1:+75696732 Msa0225580:CDS
TTTGAGGGAGGTTGAGTAGG+CGG 0.499675 2_1:-75695061 None:intergenic
TCCACAATACCAACCCTACT+AGG 0.499855 2_1:-75695741 None:intergenic
TGGTTGAACAAGTGAGAAAG+AGG 0.502404 2_1:+75698274 Msa0225580:CDS
TCTTCCTCTAGAATGGGATT+GGG 0.504186 2_1:-75695321 None:intergenic
GTTGCTGTTGGTGTTGGTGT+TGG 0.504889 2_1:+75695417 Msa0225580:CDS
CACATAGCCCTGAAGAGCTC+TGG 0.505622 2_1:-75698378 None:intergenic
AACGCTTTCGTTCTTTGCAT+CGG 0.511196 2_1:-75694990 None:intergenic
AGATCAATAAGCCCGATCCT+AGG 0.512282 2_1:+75695292 Msa0225580:CDS
ATACCAATCCTCAAATTGCC+TGG 0.513343 2_1:-75698157 None:intergenic
AGCGATGGTGGTGCTGATGC+CGG 0.513924 2_1:-75695148 None:intergenic
AGGTAATAATTCTGAAGCAT+TGG 0.515033 2_1:+75697202 Msa0225580:CDS
ACAGGCGGACTTCTAACTCT+TGG 0.517783 2_1:-75697961 None:intergenic
ATTACCATACCTAATCTTCA+GGG 0.518841 2_1:-75698586 None:intergenic
GAAGCAGCACGTTGAAGAGT+AGG 0.520003 2_1:-75695255 None:intergenic
GAAGTGGTGGTGGAGGAAGA+GGG 0.523286 2_1:-75695221 None:intergenic
AGCTAGCAACTTGTCCAAGA+TGG 0.525210 2_1:-75696746 None:intergenic
GGTGGTGCTGATGCCGGTGG+TGG 0.526394 2_1:-75695142 None:intergenic
ATCTGAACATGAGAAGCAAT+CGG 0.527639 2_1:+75697711 Msa0225580:CDS
ATCGGTGGTTGCTTCACTAA+AGG 0.528678 2_1:-75695519 None:intergenic
AGAGCCTGTTACAGCTCGAA+TGG 0.529220 2_1:+75695764 Msa0225580:CDS
TGGGCATATCGCTGAGGCTG+AGG 0.530158 2_1:+75695806 Msa0225580:CDS
GGAACAAGCTTGAATGAAGA+AGG 0.530337 2_1:+75695666 Msa0225580:CDS
AAAGTCACCTAAGGCGGCGC+CGG 0.531727 2_1:-75695088 None:intergenic
ATCCTCTTTGCCTCCATCGA+AGG 0.532894 2_1:-75695900 None:intergenic
GTTAGAAGTCCGCCTGTACA+AGG 0.533566 2_1:+75697967 Msa0225580:CDS
AGGAACTTTCCCGCAAAACC+AGG 0.533878 2_1:+75698139 Msa0225580:CDS
AAAGGCGGTGGCATAGGTGG+TGG 0.535146 2_1:-75695501 None:intergenic
TCGCATGCCAGAGCTCTTCA+GGG 0.535307 2_1:+75698371 Msa0225580:CDS
TGGTACTAGTACTGGTATGT+AGG 0.536276 2_1:-75695178 None:intergenic
GAGAAAGAGGTTAGAACATG+AGG 0.537272 2_1:+75698287 Msa0225580:CDS
GGAGAGAATTCAGGAAGTGG+TGG 0.538783 2_1:-75695234 None:intergenic
GTGTTGGTGTTGAGATTCCT+GGG 0.540980 2_1:+75695787 Msa0225580:CDS
GTAGGAGAGAATTCAGGAAG+TGG 0.543186 2_1:-75695237 None:intergenic
GGCGGACTTCTAACTCTTGG+AGG 0.543431 2_1:-75697958 None:intergenic
TGGAGTCACCGTTGCCATCA+GGG 0.544680 2_1:+75695847 Msa0225580:CDS
ATCTTCAACCCTGATGGCAA+CGG 0.545096 2_1:-75695855 None:intergenic
GAATACCTAATTTGGATGAC+GGG 0.546029 2_1:+75696683 Msa0225580:CDS
ATTGATCTTGATTTCAGGCA+TGG 0.546345 2_1:-75695277 None:intergenic
GGTGGTGGTTGATTGAGGAT+TGG 0.546928 2_1:-75695127 None:intergenic
TTCACTAAAGGCGGTGGCAT+AGG 0.548056 2_1:-75695507 None:intergenic
CTAGACGATCATGCCATGCA+TGG 0.548697 2_1:-75697996 None:intergenic
GTCTGCTTAGGAGATTGGGA+AGG 0.548931 2_1:-75697763 None:intergenic
TGAATTAGTGAATAGTGAGT+GGG 0.550993 2_1:-75694891 None:intergenic
TACAAGGTCTTCTCCATGCA+TGG 0.552507 2_1:+75697983 Msa0225580:CDS
TGCTTCATCTTCAACCCTGA+TGG 0.552691 2_1:-75695861 None:intergenic
GGAATACCTAATTTGGATGA+CGG 0.552913 2_1:+75696682 Msa0225580:CDS
ATGCTAGTCAACAATCAAAA+GGG 0.553198 2_1:+75697856 Msa0225580:CDS
ACTGATAAGTTCGCTCATGA+TGG 0.553346 2_1:-75697795 None:intergenic
TGGAGGAAGAGGGATGTCGA+AGG 0.553883 2_1:-75695211 None:intergenic
GCATACTGCAATGCCTAGTA+GGG 0.554472 2_1:+75695728 Msa0225580:CDS
AACTTCCCGATGAGCTTGCT+AGG 0.557024 2_1:+75698022 Msa0225580:CDS
GGCTTCTGAGAGTGCTCATG+AGG 0.559170 2_1:+75695989 Msa0225580:CDS
GAAGAAGGCCAACATGGAGG+AGG 0.560460 2_1:+75695681 Msa0225580:CDS
TGAATGTTCTAAAATGTACA+TGG 0.561377 2_1:+75698413 Msa0225580:CDS
GGAGGAAGAGGGATGTCGAA+GGG 0.565107 2_1:-75695210 None:intergenic
ACCTAATTTGGATGACGGGA+AGG 0.566466 2_1:+75696687 Msa0225580:CDS
TCTGCTTAGGAGATTGGGAA+GGG 0.566753 2_1:-75697762 None:intergenic
GATGGCAACGGTGACTCCAT+CGG 0.568123 2_1:-75695843 None:intergenic
GAGCCTGTTACAGCTCGAAT+GGG 0.569273 2_1:+75695765 Msa0225580:CDS
CAACACGGCGTATGATAACA+TGG 0.570122 2_1:-75698695 None:intergenic
TAACTCCTCCTCCTCCATGT+TGG 0.570608 2_1:-75695689 None:intergenic
GGTTGCTTCACTAAAGGCGG+TGG 0.573687 2_1:-75695513 None:intergenic
AGAAACGTTGCTGTTGGTGT+TGG 0.573900 2_1:+75695411 Msa0225580:CDS
AGCTGTCCTAGCAAGCTCAT+CGG 0.578087 2_1:-75698028 None:intergenic
GGAAGTGATGGAGTCGCCGA+TGG 0.578216 2_1:+75695827 Msa0225580:CDS
CTCTTTGCCTCCATCGAAGG+AGG 0.581200 2_1:-75695897 None:intergenic
TCAGCCTCAGCGATATGCCC+AGG 0.582260 2_1:-75695804 None:intergenic
GTTGAAGATGAAGCAGATGC+CGG 0.584523 2_1:+75695869 Msa0225580:CDS
GCATGGAGAAGACCTTGTAC+AGG 0.585051 2_1:-75697979 None:intergenic
ACTAAAGGCGGTGGCATAGG+TGG 0.585718 2_1:-75695504 None:intergenic
ACTGCAATGCCTAGTAGGGT+TGG 0.586333 2_1:+75695732 Msa0225580:CDS
GTTACAGCTCGAATGGGTGT+TGG 0.592701 2_1:+75695771 Msa0225580:CDS
TGAGCCCTGAAGATTAGGTA+TGG 0.593152 2_1:+75698582 Msa0225580:three_prime_UTR
GCCGTGAGAAAAGTCACCTA+AGG 0.594362 2_1:-75695097 None:intergenic
GACACATTGATCACTCCGCA+AGG 0.597277 2_1:+75696623 Msa0225580:CDS
TGAGGTTTCAAAGATGCTTG+AGG 0.598693 2_1:+75696007 Msa0225580:CDS
ACTAATAGAAACGTTGCTGT+TGG 0.599571 2_1:+75695405 Msa0225580:CDS
CTCCTTCGATGGAGGCAAAG+AGG 0.606378 2_1:+75695898 Msa0225580:CDS
TGTGACATTGCTGAGATCGT+TGG 0.608493 2_1:+75697738 Msa0225580:CDS
AATCCTCAATCAACCACCAC+CGG 0.608778 2_1:+75695129 Msa0225580:CDS
GGTATCACAAGTATATGCAA+AGG 0.609073 2_1:+75698175 Msa0225580:CDS
TTGAATGAAGAAGGCCAACA+TGG 0.609334 2_1:+75695675 Msa0225580:CDS
ACTGCAGGATGGCCTCTATG+TGG 0.618136 2_1:+75697677 Msa0225580:intron
GTAGGAATTATCAGCGATGG+TGG 0.619785 2_1:-75695160 None:intergenic
TATTGGTCGTTGTTCAACAG+TGG 0.619964 2_1:+75697818 Msa0225580:CDS
GGTTGAGTAGGCGGAGTGAG+CGG 0.621412 2_1:-75695052 None:intergenic
CAAGCAGCCGAATTATGTTG+CGG 0.621457 2_1:-75695609 None:intergenic
GCCAGAAGAAGTTGATCCAG+AGG 0.631302 2_1:+75698203 Msa0225580:CDS
TATGTAGGAATTATCAGCGA+TGG 0.639104 2_1:-75695163 None:intergenic
GGTTACGCCTCCTTCGATGG+AGG 0.643550 2_1:+75695890 Msa0225580:CDS
GGTGGTTGCTTCACTAAAGG+CGG 0.647120 2_1:-75695516 None:intergenic
CATGCCCAATCCCATTCTAG+AGG 0.649678 2_1:+75695317 Msa0225580:CDS
TGAGTAGGCGGAGTGAGCGG+CGG 0.658051 2_1:-75695049 None:intergenic
GAAATGTGAGGAAAGTCGAA+AGG 0.658419 2_1:+75698119 Msa0225580:CDS
GTGAGAAAAGTCACCTAAGG+CGG 0.659242 2_1:-75695094 None:intergenic
CTGAGCCAGAACTCATCATG+AGG 0.660589 2_1:+75698459 Msa0225580:CDS
GATGAAGAACTAGAAAACGA+AGG 0.660661 2_1:+75695363 Msa0225580:CDS
TGGAGAAGACCTTGTACAGG+CGG 0.661230 2_1:-75697976 None:intergenic
GATGGTGGTGCTGATGCCGG+TGG 0.673760 2_1:-75695145 None:intergenic
AATAACTCGCATGACCCTTG+CGG 0.676668 2_1:-75696638 None:intergenic
ACACATTGATCACTCCGCAA+GGG 0.685200 2_1:+75696624 Msa0225580:CDS
ATATGATCACTACTAATCGG+TGG 0.688134 2_1:-75695534 None:intergenic
AATGAAGAAGGCCAACATGG+AGG 0.690535 2_1:+75695678 Msa0225580:CDS
GATTCCTGGGCATATCGCTG+AGG 0.702495 2_1:+75695800 Msa0225580:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAGTTATTTTTTGTTTTTT+TGG + chr2_1:75696548-75696567 Msa0225580:intron 10.0%
!!! TAATAATAATTTTGGTATGA+TGG - chr2_1:75694836-75694855 None:intergenic 15.0%
!!! GTTTCAAGTAATAATAATTT+TGG - chr2_1:75694844-75694863 None:intergenic 15.0%
!! ACATAATAATAGTGTTAATT+TGG + chr2_1:75695929-75695948 Msa0225580:CDS 15.0%
!! TAAATCTAAATAGTTCTTTA+TGG - chr2_1:75696448-75696467 None:intergenic 15.0%
!!! ATTTTTTGTTTTTTTGGTAT+AGG + chr2_1:75696554-75696573 Msa0225580:intron 15.0%
!! AATTCATATTACTTAACATT+TGG - chr2_1:75696816-75696835 None:intergenic 15.0%
!! TAAAAAATAACACACATTTA+AGG - chr2_1:75697427-75697446 None:intergenic 15.0%
!!! TAATTTTAACCAATTACTTA+GGG - chr2_1:75697893-75697912 None:intergenic 15.0%
!!! TTAATTTTAACCAATTACTT+AGG - chr2_1:75697894-75697913 None:intergenic 15.0%
!!! TTTATTATGCTTATTTTTTG+TGG + chr2_1:75698494-75698513 Msa0225580:three_prime_UTR 15.0%
!!! TTATTATGCTTATTTTTTGT+GGG + chr2_1:75698495-75698514 Msa0225580:three_prime_UTR 15.0%
!! TTTGGAGAAAAAAAATATGA+TGG - chr2_1:75694871-75694890 None:intergenic 20.0%
!! TAAATCAAATCAAACTCAAT+GGG + chr2_1:75694928-75694947 Msa0225580:exon 20.0%
!! AAACAAAAAATAACTTACGA+GGG - chr2_1:75696545-75696564 None:intergenic 20.0%
!! AAAACAAAAAATAACTTACG+AGG - chr2_1:75696546-75696565 None:intergenic 20.0%
!!! TTTGCTTTTTTTCTTATTGA+AGG + chr2_1:75696601-75696620 Msa0225580:intron 20.0%
!! AGAAACATTTGAAAAATATG+AGG - chr2_1:75697457-75697476 None:intergenic 20.0%
!!! TGGGTAAATTATTTTTTTCT+TGG + chr2_1:75698514-75698533 Msa0225580:three_prime_UTR 20.0%
!! AAAACATGAACAAACAATAA+GGG - chr2_1:75698675-75698694 None:intergenic 20.0%
! GTAAATCAAATCAAACTCAA+TGG + chr2_1:75694927-75694946 Msa0225580:exon 25.0%
! GAATAGTAATAGAAGATTAG+AGG + chr2_1:75695461-75695480 Msa0225580:CDS 25.0%
!!! TCTTGATGATCATTTTCTTA+AGG + chr2_1:75695968-75695987 Msa0225580:CDS 25.0%
!!! CTAATTTTAAGACAGATTGA+GGG + chr2_1:75696183-75696202 Msa0225580:intron 25.0%
!!! TAATTTTAAGACAGATTGAG+GGG + chr2_1:75696184-75696203 Msa0225580:intron 25.0%
! TATTAAGGTGGATAAAATAG+AGG + chr2_1:75696382-75696401 Msa0225580:intron 25.0%
! ATCAGACATGATTTATGAAA+TGG + chr2_1:75696479-75696498 Msa0225580:intron 25.0%
! TCAGACATGATTTATGAAAT+GGG + chr2_1:75696480-75696499 Msa0225580:intron 25.0%
! AACAAAAAATAACTTACGAG+GGG - chr2_1:75696544-75696563 None:intergenic 25.0%
! CAAATTAGGTATTCCTTTAA+AGG - chr2_1:75696677-75696696 None:intergenic 25.0%
!! CTTTAAAGGAATACCTAATT+TGG + chr2_1:75696675-75696694 Msa0225580:CDS 25.0%
! GAAACTTTATGATGAAGTTA+AGG + chr2_1:75696777-75696796 Msa0225580:CDS 25.0%
! TATTGTAGATATGCAATCAT+TGG + chr2_1:75697265-75697284 Msa0225580:CDS 25.0%
!!! TAACATTTTTTTCCACATAG+AGG - chr2_1:75697692-75697711 None:intergenic 25.0%
!!! AAGATGAGATGATTTTAAAG+AGG + chr2_1:75698097-75698116 Msa0225580:CDS 25.0%
!!! TGAATGTTCTAAAATGTACA+TGG + chr2_1:75698413-75698432 Msa0225580:CDS 25.0%
!! TCTAAAATGTACATGGAATT+AGG + chr2_1:75698420-75698439 Msa0225580:CDS 25.0%
! GAAAACATGAACAAACAATA+AGG - chr2_1:75698676-75698695 None:intergenic 25.0%
!!! TTGGTATGATGGATGATTTT+GGG - chr2_1:75694825-75694844 None:intergenic 30.0%
!!! TTTGGTATGATGGATGATTT+TGG - chr2_1:75694826-75694845 None:intergenic 30.0%
TGAATTAGTGAATAGTGAGT+GGG - chr2_1:75694894-75694913 None:intergenic 30.0%
TTGAATTAGTGAATAGTGAG+TGG - chr2_1:75694895-75694914 None:intergenic 30.0%
CCAATATGATCACTACTAAT+CGG - chr2_1:75695540-75695559 None:intergenic 30.0%
!!! GGAGTATTTTTTTCCATCAA+TGG + chr2_1:75695632-75695651 Msa0225580:CDS 30.0%
!!! GTATTTTTTTCCATCAATGG+AGG + chr2_1:75695635-75695654 Msa0225580:CDS 30.0%
! TCCAATTTTGCTGATAATAG+AGG + chr2_1:75696050-75696069 Msa0225580:CDS 30.0%
ACCTCTATTATCAGCAAAAT+TGG - chr2_1:75696054-75696073 None:intergenic 30.0%
!!! GCTAATTTTAAGACAGATTG+AGG + chr2_1:75696182-75696201 Msa0225580:intron 30.0%
!! AGTTCTAGAGTTAAAAGCAA+AGG + chr2_1:75696407-75696426 Msa0225580:intron 30.0%
CAGACATGATTTATGAAATG+GGG + chr2_1:75696481-75696500 Msa0225580:intron 30.0%
ATTATACAAAACTCCTACCA+TGG - chr2_1:75696898-75696917 None:intergenic 30.0%
!! GGAGTTTTGTATAATCAAGT+TGG + chr2_1:75696903-75696922 Msa0225580:intron 30.0%
TCCACTTCATATAATGTTGT+AGG + chr2_1:75697115-75697134 Msa0225580:intron 30.0%
!! AGGTAATAATTCTGAAGCAT+TGG + chr2_1:75697202-75697221 Msa0225580:CDS 30.0%
TATAGTAATGTTTCCCTCAT+GGG + chr2_1:75697488-75697507 Msa0225580:intron 30.0%
TTTAAACAAGTATCCCATGA+GGG - chr2_1:75697504-75697523 None:intergenic 30.0%
TAAGTTGCATGATTCTTGTA+GGG + chr2_1:75697541-75697560 Msa0225580:intron 30.0%
!!! TAGTTTTTTCTTTTGACTGC+AGG + chr2_1:75697662-75697681 Msa0225580:intron 30.0%
ATGCTAGTCAACAATCAAAA+GGG + chr2_1:75697856-75697875 Msa0225580:CDS 30.0%
ACATAAAGTCCCTAAGTAAT+TGG + chr2_1:75697881-75697900 Msa0225580:CDS 30.0%
!! GATTTTAAAGAGGAAATGTG+AGG + chr2_1:75698107-75698126 Msa0225580:CDS 30.0%
TTCTCTGTAACAATTTCATC+AGG - chr2_1:75698246-75698265 None:intergenic 30.0%
CAAGTAAGGCAAAAAACTTT+GGG + chr2_1:75698336-75698355 Msa0225580:CDS 30.0%
! TCTTGAAATCTCTTGATTTG+TGG + chr2_1:75698558-75698577 Msa0225580:three_prime_UTR 30.0%
ATTACCATACCTAATCTTCA+GGG - chr2_1:75698589-75698608 None:intergenic 30.0%
GAAACTCTTGTCTTTGTCTT+TGG + chr2_1:75694742-75694761 Msa0225580:five_prime_UTR 35.0%
TATGTAGGAATTATCAGCGA+TGG - chr2_1:75695166-75695185 None:intergenic 35.0%
ATTGATCTTGATTTCAGGCA+TGG - chr2_1:75695280-75695299 None:intergenic 35.0%
GGCTTATTGATCTTGATTTC+AGG - chr2_1:75695285-75695304 None:intergenic 35.0%
!! TCATCTTCTTCCTCTAGAAT+GGG - chr2_1:75695330-75695349 None:intergenic 35.0%
! ATCATCTTCTTCCTCTAGAA+TGG - chr2_1:75695331-75695350 None:intergenic 35.0%
GATGAAGAACTAGAAAACGA+AGG + chr2_1:75695363-75695382 Msa0225580:CDS 35.0%
ACTAATAGAAACGTTGCTGT+TGG + chr2_1:75695405-75695424 Msa0225580:CDS 35.0%
ATATGATCACTACTAATCGG+TGG - chr2_1:75695537-75695556 None:intergenic 35.0%
CCGATTAGTAGTGATCATAT+TGG + chr2_1:75695537-75695556 Msa0225580:CDS 35.0%
!! TGATAATAGAGGTACTCTAC+TGG + chr2_1:75696061-75696080 Msa0225580:intron 35.0%
!! GATAATAGAGGTACTCTACT+GGG + chr2_1:75696062-75696081 Msa0225580:intron 35.0%
! ATTTTGCCTTTTCACAAGAG+TGG + chr2_1:75696291-75696310 Msa0225580:intron 35.0%
! TCATTGAATGTTGCCTTGTT+TGG + chr2_1:75696321-75696340 Msa0225580:intron 35.0%
TTGTATGGCATAACCAAACA+AGG - chr2_1:75696337-75696356 None:intergenic 35.0%
!! TCTGTGTCACTTAGTTTATG+TGG + chr2_1:75696514-75696533 Msa0225580:intron 35.0%
ACTTGGAATCGATCCTTTAA+AGG + chr2_1:75696661-75696680 Msa0225580:CDS 35.0%
GGAATACCTAATTTGGATGA+CGG + chr2_1:75696682-75696701 Msa0225580:CDS 35.0%
GAATACCTAATTTGGATGAC+GGG + chr2_1:75696683-75696702 Msa0225580:CDS 35.0%
! ACAATTTTCTTACAGAGTCG+TGG + chr2_1:75696867-75696886 Msa0225580:intron 35.0%
GCCTACAACATTATATGAAG+TGG - chr2_1:75697119-75697138 None:intergenic 35.0%
CTTCATATAATGTTGTAGGC+TGG + chr2_1:75697119-75697138 Msa0225580:intron 35.0%
! TTAGTGAGGCAACTTTTCTT+TGG - chr2_1:75697166-75697185 None:intergenic 35.0%
!!! TTTTTTCAGCCTGTTTAGTG+AGG - chr2_1:75697180-75697199 None:intergenic 35.0%
CTAAACAGGCTGAAAAAAAG+AGG + chr2_1:75697182-75697201 Msa0225580:CDS 35.0%
ACAATTGTTCATCGCGTAAT+CGG - chr2_1:75697313-75697332 None:intergenic 35.0%
TCAACAAGCTTAACAAGTCT+TGG - chr2_1:75697338-75697357 None:intergenic 35.0%
ACTACAAGACTACTAGACAT+GGG - chr2_1:75697399-75697418 None:intergenic 35.0%
AACTACAAGACTACTAGACA+TGG - chr2_1:75697400-75697419 None:intergenic 35.0%
CTATAGTAATGTTTCCCTCA+TGG + chr2_1:75697487-75697506 Msa0225580:intron 35.0%
GTTTAAACAAGTATCCCATG+AGG - chr2_1:75697505-75697524 None:intergenic 35.0%
GTAAGTTGCATGATTCTTGT+AGG + chr2_1:75697540-75697559 Msa0225580:intron 35.0%
GATCTGGACTCAAGTATTAA+TGG - chr2_1:75697587-75697606 None:intergenic 35.0%
ATTGTGCATTCATACAGATC+TGG - chr2_1:75697603-75697622 None:intergenic 35.0%
!! TTTTTCTTTTGACTGCAGGA+TGG + chr2_1:75697666-75697685 Msa0225580:intron 35.0%
ATCTGAACATGAGAAGCAAT+CGG + chr2_1:75697711-75697730 Msa0225580:CDS 35.0%
TGAGCGAACTTATCAGTTAT+TGG + chr2_1:75697801-75697820 Msa0225580:CDS 35.0%
GATGCTAGTCAACAATCAAA+AGG + chr2_1:75697855-75697874 Msa0225580:CDS 35.0%
CCTTTAAACTGCTCTCAATA+GGG - chr2_1:75697924-75697943 None:intergenic 35.0%
TCCTTTAAACTGCTCTCAAT+AGG - chr2_1:75697925-75697944 None:intergenic 35.0%
GGTATCACAAGTATATGCAA+AGG + chr2_1:75698175-75698194 Msa0225580:CDS 35.0%
! AACAATTTCATCAGGAGCAT+TGG - chr2_1:75698238-75698257 None:intergenic 35.0%
! TGTTACAGAGAAGCAGTTTT+TGG + chr2_1:75698254-75698273 Msa0225580:CDS 35.0%
!!! AAGTTTTTTGCCTTACTTGG+AGG - chr2_1:75698335-75698354 None:intergenic 35.0%
!!! CCAAAGTTTTTTGCCTTACT+TGG - chr2_1:75698338-75698357 None:intergenic 35.0%
CCAAGTAAGGCAAAAAACTT+TGG + chr2_1:75698335-75698354 Msa0225580:CDS 35.0%
CATTACCATACCTAATCTTC+AGG - chr2_1:75698590-75698609 None:intergenic 35.0%
! TAGTGAATAGTGAGTGGGTT+TGG - chr2_1:75694889-75694908 None:intergenic 40.0%
AACGCTTTCGTTCTTTGCAT+CGG - chr2_1:75694993-75695012 None:intergenic 40.0%
!! TGGTACTAGTACTGGTATGT+AGG - chr2_1:75695181-75695200 None:intergenic 40.0%
! TTTTGTGGTGGTACTAGTAC+TGG - chr2_1:75695189-75695208 None:intergenic 40.0%
TGAAGAGTAGGAGAGAATTC+AGG - chr2_1:75695246-75695265 None:intergenic 40.0%
!! TCTTCCTCTAGAATGGGATT+GGG - chr2_1:75695324-75695343 None:intergenic 40.0%
!! TTCTTCCTCTAGAATGGGAT+TGG - chr2_1:75695325-75695344 None:intergenic 40.0%
TAGAAAACGAAGGCTCTTTG+AGG + chr2_1:75695373-75695392 Msa0225580:CDS 40.0%
ATTATGTTGCGGATTCGGAT+TGG - chr2_1:75695601-75695620 None:intergenic 40.0%
TCCGAATCCGCAACATAATT+CGG + chr2_1:75695602-75695621 Msa0225580:CDS 40.0%
GGAACAAGCTTGAATGAAGA+AGG + chr2_1:75695666-75695685 Msa0225580:CDS 40.0%
TTGAATGAAGAAGGCCAACA+TGG + chr2_1:75695675-75695694 Msa0225580:CDS 40.0%
TGAGGTTTCAAAGATGCTTG+AGG + chr2_1:75696007-75696026 Msa0225580:CDS 40.0%
TTAAGACAGATTGAGGGGAA+TGG + chr2_1:75696189-75696208 Msa0225580:intron 40.0%
GCTCATGTCTTCTCTTTGTA+TGG - chr2_1:75696352-75696371 None:intergenic 40.0%
TACAAAGAGAAGACATGAGC+AGG + chr2_1:75696352-75696371 Msa0225580:intron 40.0%
TGAGCAGGCACTAGTTATTA+AGG + chr2_1:75696367-75696386 Msa0225580:intron 40.0%
TGAAACTCATGCTACCATCT+TGG + chr2_1:75696732-75696751 Msa0225580:CDS 40.0%
TAGAGCAGTAGGAAAATGAC+TGG - chr2_1:75697017-75697036 None:intergenic 40.0%
GATACACACAAACACACACA+TGG - chr2_1:75697071-75697090 None:intergenic 40.0%
GAAAAGTTGCCTCACTAAAC+AGG + chr2_1:75697168-75697187 Msa0225580:CDS 40.0%
ACTTGTCTGCTTAGGAGATT+GGG - chr2_1:75697770-75697789 None:intergenic 40.0%
ACTGATAAGTTCGCTCATGA+TGG - chr2_1:75697798-75697817 None:intergenic 40.0%
TATTGGTCGTTGTTCAACAG+TGG + chr2_1:75697818-75697837 Msa0225580:CDS 40.0%
CCCTATTGAGAGCAGTTTAA+AGG + chr2_1:75697921-75697940 Msa0225580:CDS 40.0%
GAAATGTGAGGAAAGTCGAA+AGG + chr2_1:75698119-75698138 Msa0225580:CDS 40.0%
AAACCAGGCAATTTGAGGAT+TGG + chr2_1:75698154-75698173 Msa0225580:CDS 40.0%
ATACCAATCCTCAAATTGCC+TGG - chr2_1:75698160-75698179 None:intergenic 40.0%
TGGTTGAACAAGTGAGAAAG+AGG + chr2_1:75698274-75698293 Msa0225580:CDS 40.0%
GAGAAAGAGGTTAGAACATG+AGG + chr2_1:75698287-75698306 Msa0225580:CDS 40.0%
AAAAGCAATGCCTCCAAGTA+AGG + chr2_1:75698322-75698341 Msa0225580:CDS 40.0%
! AACTCATCATGAGGTGCTTT+GGG + chr2_1:75698468-75698487 Msa0225580:exon 40.0%
!! GTTTTTGAGGGAGGTTGAGT+AGG - chr2_1:75695067-75695086 None:intergenic 45.0%
TCAACCTCCCTCAAAAACAC+CGG + chr2_1:75695069-75695088 Msa0225580:CDS 45.0%
GTGAGAAAAGTCACCTAAGG+CGG - chr2_1:75695097-75695116 None:intergenic 45.0%
! GCCTTAGGTGACTTTTCTCA+CGG + chr2_1:75695096-75695115 Msa0225580:CDS 45.0%
AATCCTCAATCAACCACCAC+CGG + chr2_1:75695129-75695148 Msa0225580:CDS 45.0%
GTAGGAATTATCAGCGATGG+TGG - chr2_1:75695163-75695182 None:intergenic 45.0%
! ATGTCGAAGGGTTTTTGTGG+TGG - chr2_1:75695201-75695220 None:intergenic 45.0%
GTAGGAGAGAATTCAGGAAG+TGG - chr2_1:75695240-75695259 None:intergenic 45.0%
AGATCAATAAGCCCGATCCT+AGG + chr2_1:75695292-75695311 Msa0225580:CDS 45.0%
! TCTAGAATGGGATTGGGCAT+GGG - chr2_1:75695318-75695337 None:intergenic 45.0%
! AGAAACGTTGCTGTTGGTGT+TGG + chr2_1:75695411-75695430 Msa0225580:CDS 45.0%
ATCGGTGGTTGCTTCACTAA+AGG - chr2_1:75695522-75695541 None:intergenic 45.0%
GCCGAATTATGTTGCGGATT+CGG - chr2_1:75695606-75695625 None:intergenic 45.0%
CAAGCAGCCGAATTATGTTG+CGG - chr2_1:75695612-75695631 None:intergenic 45.0%
GCAACATAATTCGGCTGCTT+GGG + chr2_1:75695611-75695630 Msa0225580:CDS 45.0%
CCTGCAATATCCTCCATTGA+TGG - chr2_1:75695648-75695667 None:intergenic 45.0%
CCATCAATGGAGGATATTGC+AGG + chr2_1:75695645-75695664 Msa0225580:CDS 45.0%
AATGAAGAAGGCCAACATGG+AGG + chr2_1:75695678-75695697 Msa0225580:CDS 45.0%
AGCATACTGCAATGCCTAGT+AGG + chr2_1:75695727-75695746 Msa0225580:CDS 45.0%
GCATACTGCAATGCCTAGTA+GGG + chr2_1:75695728-75695747 Msa0225580:CDS 45.0%
TCCACAATACCAACCCTACT+AGG - chr2_1:75695744-75695763 None:intergenic 45.0%
!! GTGTTGGTGTTGAGATTCCT+GGG + chr2_1:75695787-75695806 Msa0225580:CDS 45.0%
ATCTTCAACCCTGATGGCAA+CGG - chr2_1:75695858-75695877 None:intergenic 45.0%
TGCTTCATCTTCAACCCTGA+TGG - chr2_1:75695864-75695883 None:intergenic 45.0%
GTTGAAGATGAAGCAGATGC+CGG + chr2_1:75695869-75695888 Msa0225580:CDS 45.0%
CAAAGATGCTTGAGGCTACA+AGG + chr2_1:75696015-75696034 Msa0225580:CDS 45.0%
GTTGCACTAGATAGAGACTC+TGG + chr2_1:75696084-75696103 Msa0225580:intron 45.0%
GTCTAGCCACTCTTGTGAAA+AGG - chr2_1:75696300-75696319 None:intergenic 45.0%
GCAGGCACTAGTTATTAAGG+TGG + chr2_1:75696370-75696389 Msa0225580:intron 45.0%
ACACATTGATCACTCCGCAA+GGG + chr2_1:75696624-75696643 Msa0225580:CDS 45.0%
AATAACTCGCATGACCCTTG+CGG - chr2_1:75696641-75696660 None:intergenic 45.0%
GGGTCATGCGAGTTATTACT+TGG + chr2_1:75696644-75696663 Msa0225580:CDS 45.0%
!! ACCTAATTTGGATGACGGGA+AGG + chr2_1:75696687-75696706 Msa0225580:CDS 45.0%
TCCTTCCCGTCATCCAAATT+AGG - chr2_1:75696691-75696710 None:intergenic 45.0%
AGCTAGCAACTTGTCCAAGA+TGG - chr2_1:75696749-75696768 None:intergenic 45.0%
TCTTGGACAAGTTGCTAGCT+TGG + chr2_1:75696749-75696768 Msa0225580:CDS 45.0%
! CTTGGACAAGTTGCTAGCTT+GGG + chr2_1:75696750-75696769 Msa0225580:CDS 45.0%
ACAAAACTCCTACCATGGCA+TGG - chr2_1:75696893-75696912 None:intergenic 45.0%
TCAAGCGAAGATAGAGCAGT+AGG - chr2_1:75697028-75697047 None:intergenic 45.0%
CAATTGACTGACAGCTGCTT+TGG - chr2_1:75697234-75697253 None:intergenic 45.0%
GCAGAGAAGCTTGTACTGTA+TGG - chr2_1:75697633-75697652 None:intergenic 45.0%
TGTGACATTGCTGAGATCGT+TGG + chr2_1:75697738-75697757 Msa0225580:CDS 45.0%
TCTGCTTAGGAGATTGGGAA+GGG - chr2_1:75697765-75697784 None:intergenic 45.0%
CACTTGTCTGCTTAGGAGAT+TGG - chr2_1:75697771-75697790 None:intergenic 45.0%
TGGTGTTCACTTGTCTGCTT+AGG - chr2_1:75697778-75697797 None:intergenic 45.0%
TACAAGGTCTTCTCCATGCA+TGG + chr2_1:75697983-75698002 Msa0225580:CDS 45.0%
!!! CTCAAATTGCCTGGTTTTGC+GGG - chr2_1:75698151-75698170 None:intergenic 45.0%
!!! CCTCAAATTGCCTGGTTTTG+CGG - chr2_1:75698152-75698171 None:intergenic 45.0%
AAGCACCTCATGATGAGTTC+TGG - chr2_1:75698467-75698486 None:intergenic 45.0%
! GAACTCATCATGAGGTGCTT+TGG + chr2_1:75698467-75698486 Msa0225580:exon 45.0%
TGAGCCCTGAAGATTAGGTA+TGG + chr2_1:75698582-75698601 Msa0225580:three_prime_UTR 45.0%
!! TTCATATTAAATAATATTTA+TGG + chr2_1:75696578-75696597 Msa0225580:intron 5.0%
TTTGAGGGAGGTTGAGTAGG+CGG - chr2_1:75695064-75695083 None:intergenic 50.0%
GCCGTGAGAAAAGTCACCTA+AGG - chr2_1:75695100-75695119 None:intergenic 50.0%
! GGTGGTGGTTGATTGAGGAT+TGG - chr2_1:75695130-75695149 None:intergenic 50.0%
! GGGATGTCGAAGGGTTTTTG+TGG - chr2_1:75695204-75695223 None:intergenic 50.0%
!! AATTCAGGAAGTGGTGGTGG+AGG - chr2_1:75695231-75695250 None:intergenic 50.0%
GAGAATTCAGGAAGTGGTGG+TGG - chr2_1:75695234-75695253 None:intergenic 50.0%
GGAGAGAATTCAGGAAGTGG+TGG - chr2_1:75695237-75695256 None:intergenic 50.0%
GAAGCAGCACGTTGAAGAGT+AGG - chr2_1:75695258-75695277 None:intergenic 50.0%
!! CTCTAGAATGGGATTGGGCA+TGG - chr2_1:75695319-75695338 None:intergenic 50.0%
CATGCCCAATCCCATTCTAG+AGG + chr2_1:75695317-75695336 Msa0225580:CDS 50.0%
!! GTTGCTGTTGGTGTTGGTGT+TGG + chr2_1:75695417-75695436 Msa0225580:CDS 50.0%
!! GTTGGTGTTGGTGTTGGTGT+TGG + chr2_1:75695423-75695442 Msa0225580:CDS 50.0%
TTCACTAAAGGCGGTGGCAT+AGG - chr2_1:75695510-75695529 None:intergenic 50.0%
GGTGGTTGCTTCACTAAAGG+CGG - chr2_1:75695519-75695538 None:intergenic 50.0%
CGCAACATAATTCGGCTGCT+TGG + chr2_1:75695610-75695629 Msa0225580:CDS 50.0%
TAACTCCTCCTCCTCCATGT+TGG - chr2_1:75695692-75695711 None:intergenic 50.0%
ACTGCAATGCCTAGTAGGGT+TGG + chr2_1:75695732-75695751 Msa0225580:CDS 50.0%
!! GCCTAGTAGGGTTGGTATTG+TGG + chr2_1:75695740-75695759 Msa0225580:CDS 50.0%
AGAGCCTGTTACAGCTCGAA+TGG + chr2_1:75695764-75695783 Msa0225580:CDS 50.0%
GAGCCTGTTACAGCTCGAAT+GGG + chr2_1:75695765-75695784 Msa0225580:CDS 50.0%
ACACCCATTCGAGCTGTAAC+AGG - chr2_1:75695771-75695790 None:intergenic 50.0%
GTTACAGCTCGAATGGGTGT+TGG + chr2_1:75695771-75695790 Msa0225580:CDS 50.0%
!! GGTGTTGGTGTTGAGATTCC+TGG + chr2_1:75695786-75695805 Msa0225580:CDS 50.0%
ATCCTCTTTGCCTCCATCGA+AGG - chr2_1:75695903-75695922 None:intergenic 50.0%
GACACATTGATCACTCCGCA+AGG + chr2_1:75696623-75696642 Msa0225580:CDS 50.0%
GTCTGCTTAGGAGATTGGGA+AGG - chr2_1:75697766-75697785 None:intergenic 50.0%
! ACAGGCGGACTTCTAACTCT+TGG - chr2_1:75697964-75697983 None:intergenic 50.0%
GTTAGAAGTCCGCCTGTACA+AGG + chr2_1:75697967-75697986 Msa0225580:CDS 50.0%
TGGAGAAGACCTTGTACAGG+CGG - chr2_1:75697979-75697998 None:intergenic 50.0%
GCATGGAGAAGACCTTGTAC+AGG - chr2_1:75697982-75698001 None:intergenic 50.0%
CTAGACGATCATGCCATGCA+TGG - chr2_1:75697999-75698018 None:intergenic 50.0%
! AACTTCCCGATGAGCTTGCT+AGG + chr2_1:75698022-75698041 Msa0225580:CDS 50.0%
AGCTGTCCTAGCAAGCTCAT+CGG - chr2_1:75698031-75698050 None:intergenic 50.0%
!! GAGCTTGCTAGGACAGCTAT+AGG + chr2_1:75698033-75698052 Msa0225580:CDS 50.0%
AGGAACTTTCCCGCAAAACC+AGG + chr2_1:75698139-75698158 Msa0225580:CDS 50.0%
CCGCAAAACCAGGCAATTTG+AGG + chr2_1:75698149-75698168 Msa0225580:CDS 50.0%
! GCCAGAAGAAGTTGATCCAG+AGG + chr2_1:75698203-75698222 Msa0225580:CDS 50.0%
GCCTCTGGATCAACTTCTTC+TGG - chr2_1:75698207-75698226 None:intergenic 50.0%
CTGAGCCAGAACTCATCATG+AGG + chr2_1:75698459-75698478 Msa0225580:CDS 50.0%
GTGGATGAGCCCTGAAGATT+AGG + chr2_1:75698577-75698596 Msa0225580:three_prime_UTR 50.0%
! ATGCCGGTGGTGGTTGATTG+AGG - chr2_1:75695135-75695154 None:intergenic 55.0%
GGAGGAAGAGGGATGTCGAA+GGG - chr2_1:75695213-75695232 None:intergenic 55.0%
TGGAGGAAGAGGGATGTCGA+AGG - chr2_1:75695214-75695233 None:intergenic 55.0%
!! GAAGTGGTGGTGGAGGAAGA+GGG - chr2_1:75695224-75695243 None:intergenic 55.0%
ATTGGGCATGGGCCTAGGAT+CGG - chr2_1:75695307-75695326 None:intergenic 55.0%
!! GTTGGTGTTGGTGTTGGTGC+TGG + chr2_1:75695429-75695448 Msa0225580:CDS 55.0%
ACTAAAGGCGGTGGCATAGG+TGG - chr2_1:75695507-75695526 None:intergenic 55.0%
GGTTGCTTCACTAAAGGCGG+TGG - chr2_1:75695516-75695535 None:intergenic 55.0%
GAAGAAGGCCAACATGGAGG+AGG + chr2_1:75695681-75695700 Msa0225580:CDS 55.0%
GATTCCTGGGCATATCGCTG+AGG + chr2_1:75695800-75695819 Msa0225580:CDS 55.0%
GATGGCAACGGTGACTCCAT+CGG - chr2_1:75695846-75695865 None:intergenic 55.0%
ATGGAGTCACCGTTGCCATC+AGG + chr2_1:75695846-75695865 Msa0225580:CDS 55.0%
TGGAGTCACCGTTGCCATCA+GGG + chr2_1:75695847-75695866 Msa0225580:CDS 55.0%
! TCCATCGAAGGAGGCGTAAC+CGG - chr2_1:75695891-75695910 None:intergenic 55.0%
CTCTTTGCCTCCATCGAAGG+AGG - chr2_1:75695900-75695919 None:intergenic 55.0%
CTCCTTCGATGGAGGCAAAG+AGG + chr2_1:75695898-75695917 Msa0225580:CDS 55.0%
!! GGCTTCTGAGAGTGCTCATG+AGG + chr2_1:75695989-75696008 Msa0225580:CDS 55.0%
ACTGCAGGATGGCCTCTATG+TGG + chr2_1:75697677-75697696 Msa0225580:intron 55.0%
! GGCGGACTTCTAACTCTTGG+AGG - chr2_1:75697961-75697980 None:intergenic 55.0%
GCTGTCCTAGCAAGCTCATC+GGG - chr2_1:75698030-75698049 None:intergenic 55.0%
! GCATTGGCATCTTCAGCCTC+TGG - chr2_1:75698222-75698241 None:intergenic 55.0%
ATCGCATGCCAGAGCTCTTC+AGG + chr2_1:75698370-75698389 Msa0225580:CDS 55.0%
TCGCATGCCAGAGCTCTTCA+GGG + chr2_1:75698371-75698390 Msa0225580:CDS 55.0%
CACATAGCCCTGAAGAGCTC+TGG - chr2_1:75698381-75698400 None:intergenic 55.0%
GGTTGAGTAGGCGGAGTGAG+CGG - chr2_1:75695055-75695074 None:intergenic 60.0%
AAAAACACCGGCGCCGCCTT+AGG + chr2_1:75695081-75695100 Msa0225580:CDS 60.0%
AAAGTCACCTAAGGCGGCGC+CGG - chr2_1:75695091-75695110 None:intergenic 60.0%
!! AGCGATGGTGGTGCTGATGC+CGG - chr2_1:75695151-75695170 None:intergenic 60.0%
!! GGAAGTGGTGGTGGAGGAAG+AGG - chr2_1:75695225-75695244 None:intergenic 60.0%
TTGGGCATGGGCCTAGGATC+GGG - chr2_1:75695306-75695325 None:intergenic 60.0%
ATGGGATTGGGCATGGGCCT+AGG - chr2_1:75695312-75695331 None:intergenic 60.0%
!! GGTGTTGGTGTTGGTGCTGG+TGG + chr2_1:75695432-75695451 Msa0225580:CDS 60.0%
!! GTTGGTGTTGGTGCTGGTGG+AGG + chr2_1:75695435-75695454 Msa0225580:CDS 60.0%
AAAGGCGGTGGCATAGGTGG+TGG - chr2_1:75695504-75695523 None:intergenic 60.0%
GAAGGCCAACATGGAGGAGG+AGG + chr2_1:75695684-75695703 Msa0225580:CDS 60.0%
TCAGCCTCAGCGATATGCCC+AGG - chr2_1:75695807-75695826 None:intergenic 60.0%
TGGGCATATCGCTGAGGCTG+AGG + chr2_1:75695806-75695825 Msa0225580:CDS 60.0%
CGCTGAGGCTGAGGAAGTGA+TGG + chr2_1:75695815-75695834 Msa0225580:CDS 60.0%
! GGAAGTGATGGAGTCGCCGA+TGG + chr2_1:75695827-75695846 Msa0225580:CDS 60.0%
GGTTACGCCTCCTTCGATGG+AGG + chr2_1:75695890-75695909 Msa0225580:CDS 60.0%
ACAGAGTCGTGGCCATGCCA+TGG + chr2_1:75696878-75696897 Msa0225580:intron 60.0%
AGTCGTGGCCATGCCATGGT+AGG + chr2_1:75696882-75696901 Msa0225580:intron 60.0%
TGAGTAGGCGGAGTGAGCGG+CGG - chr2_1:75695052-75695071 None:intergenic 65.0%
!!! GGCGCCGGTGTTTTTGAGGG+AGG - chr2_1:75695076-75695095 None:intergenic 65.0%
!!! GGCGGCGCCGGTGTTTTTGA+GGG - chr2_1:75695079-75695098 None:intergenic 65.0%
!!! AGGCGGCGCCGGTGTTTTTG+AGG - chr2_1:75695080-75695099 None:intergenic 65.0%
!! GATGGTGGTGCTGATGCCGG+TGG - chr2_1:75695148-75695167 None:intergenic 65.0%
GCCGGTTACGCCTCCTTCGA+TGG + chr2_1:75695887-75695906 Msa0225580:CDS 65.0%
!! GGTGGTGCTGATGCCGGTGG+TGG - chr2_1:75695145-75695164 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 75694723 75698716 75694723 ID=Msa0225580;Name=Msa0225580
chr2_1 mRNA 75694723 75698716 75694723 ID=Msa0225580-mRNA-1;Parent=Msa0225580;Name=Msa0225580-mRNA-1;_AED=0.02;_eAED=0.02;_QI=223|1|1|1|1|1|4|237|773
chr2_1 exon 75694723 75696071 75694723 ID=Msa0225580-mRNA-1:exon:22706;Parent=Msa0225580-mRNA-1
chr2_1 exon 75696623 75696798 75696623 ID=Msa0225580-mRNA-1:exon:22707;Parent=Msa0225580-mRNA-1
chr2_1 exon 75697137 75697360 75697137 ID=Msa0225580-mRNA-1:exon:22708;Parent=Msa0225580-mRNA-1
chr2_1 exon 75697684 75698716 75697684 ID=Msa0225580-mRNA-1:exon:22709;Parent=Msa0225580-mRNA-1
chr2_1 five_prime_UTR 75694723 75694945 75694723 ID=Msa0225580-mRNA-1:five_prime_utr;Parent=Msa0225580-mRNA-1
chr2_1 CDS 75694946 75696071 75694946 ID=Msa0225580-mRNA-1:cds;Parent=Msa0225580-mRNA-1
chr2_1 CDS 75696623 75696798 75696623 ID=Msa0225580-mRNA-1:cds;Parent=Msa0225580-mRNA-1
chr2_1 CDS 75697137 75697360 75697137 ID=Msa0225580-mRNA-1:cds;Parent=Msa0225580-mRNA-1
chr2_1 CDS 75697684 75698479 75697684 ID=Msa0225580-mRNA-1:cds;Parent=Msa0225580-mRNA-1
chr2_1 three_prime_UTR 75698480 75698716 75698480 ID=Msa0225580-mRNA-1:three_prime_utr;Parent=Msa0225580-mRNA-1
Gene Sequence

>Msa0225580

ATGGGTTGTAATCAATCGAAGATCGAGAACGAAGAATCAATTCTCCGATGCAAAGAACGAAAGCGTTACATGAAAGAAGCTGTTTCATCTCGTAATGCTTTCGCCGCCGCTCACTCCGCCTACTCAACCTCCCTCAAAAACACCGGCGCCGCCTTAGGTGACTTTTCTCACGGCGAAGTCGCCAATCCTCAATCAACCACCACCGGCATCAGCACCACCATCGCTGATAATTCCTACATACCAGTACTAGTACCACCACAAAAACCCTTCGACATCCCTCTTCCTCCACCACCACTTCCTGAATTCTCTCCTACTCTTCAACGTGCTGCTTCCATGCCTGAAATCAAGATCAATAAGCCCGATCCTAGGCCCATGCCCAATCCCATTCTAGAGGAAGAAGATGATGATGATGATGATGATGAAGAACTAGAAAACGAAGGCTCTTTGAGGAAAAGAAGAACTAATAGAAACGTTGCTGTTGGTGTTGGTGTTGGTGTTGGTGCTGGTGGAGGTGTGAATAGTAATAGAAGATTAGAGGATGAAGAAGTTGAAGCTCCACCACCTATGCCACCGCCTTTAGTGAAGCAACCACCGATTAGTAGTGATCATATTGGTAATAACAATCAAAGTCATCATCATCATCATACTATGTCCAATCCGAATCCGCAACATAATTCGGCTGCTTGGGAGTATTTTTTTCCATCAATGGAGGATATTGCAGGAACAAGCTTGAATGAAGAAGGCCAACATGGAGGAGGAGGAGTTACATTCAATAGAATGCAGCATACTGCAATGCCTAGTAGGGTTGGTATTGTGGAAGAGCCTGTTACAGCTCGAATGGGTGTTGGTGTTGAGATTCCTGGGCATATCGCTGAGGCTGAGGAAGTGATGGAGTCGCCGATGGAGTCACCGTTGCCATCAGGGTTGAAGATGAAGCAGATGCCGGTTACGCCTCCTTCGATGGAGGCAAAGAGGATTGTGAAACATAATAATAGTGTTAATTTGGTTCAGATATTTACTGATCTTGATGATCATTTTCTTAAGGCTTCTGAGAGTGCTCATGAGGTTTCAAAGATGCTTGAGGCTACAAGGTTGCACTATCACTCCAATTTTGCTGATAATAGAGGACACATTGATCACTCCGCAAGGGTCATGCGAGTTATTACTTGGAATCGATCCTTTAAAGGAATACCTAATTTGGATGACGGGAAGGATGATTTTGACTCAGATGAGCATGAAACTCATGCTACCATCTTGGACAAGTTGCTAGCTTGGGAAAAGAAACTTTATGATGAAGTTAAGGCTGGTGAACTAATGAAATTTGATTACCAAAGAAAAGTTGCCTCACTAAACAGGCTGAAAAAAAGAGGTAATAATTCTGAAGCATTGGAGAAAGCCAAAGCAGCTGTCAGTCAATTGCATACAAGATATATTGTAGATATGCAATCATTGGATTCGACAGTCTCAGAGATTAACCGATTACGCGATGAACAATTGTATCCAAGACTTGTTAAGCTTGTTGAAGAGATGGCCTCTATGTGGAAAAAAATGTTATCTGAACATGAGAAGCAATCGGAGACTGTGACATTGCTGAGATCGTTGGACCCTTCCCAATCTCCTAAGCAGACAAGTGAACACCATCATGAGCGAACTTATCAGTTATTGGTCGTTGTTCAACAGTGGCATTTACAATTTGAGATGCTAGTCAACAATCAAAAGGGATACATAAAGTCCCTAAGTAATTGGTTAAAATTAAATCTTATCCCTATTGAGAGCAGTTTAAAGGAGAAAGTTTCTTCACCTCCAAGAGTTAGAAGTCCGCCTGTACAAGGTCTTCTCCATGCATGGCATGATCGTCTAGAGAAACTTCCCGATGAGCTTGCTAGGACAGCTATAGGAAACTTTGCTGCTGTGATAGATACTATTTTCAATCAACAAGAAGATGAGATGATTTTAAAGAGGAAATGTGAGGAAAGTCGAAAGGAACTTTCCCGCAAAACCAGGCAATTTGAGGATTGGTATCACAAGTATATGCAAAGGAAAATGCCAGAAGAAGTTGATCCAGAGGCTGAAGATGCCAATGCTCCTGATGAAATTGTTACAGAGAAGCAGTTTTTGGTTGAACAAGTGAGAAAGAGGTTAGAACATGAGGAAGCAGAATATGAAAAGCAATGCCTCCAAGTAAGGCAAAAAACTTTGGGAAGTCTCAAAAATCGCATGCCAGAGCTCTTCAGGGCTATGTGTGATTTTTCTCTTGAATGTTCTAAAATGTACATGGAATTAGGTTCGATATCGCAGCATCTGAGCCAGAACTCATCATGA
Protein sequence

>Msa0225580

MGCNQSKIENEESILRCKERKRYMKEAVSSRNAFAAAHSAYSTSLKNTGAALGDFSHGEVANPQSTTTGISTTIADNSYIPVLVPPQKPFDIPLPPPPLPEFSPTLQRAASMPEIKINKPDPRPMPNPILEEEDDDDDDDEELENEGSLRKRRTNRNVAVGVGVGVGAGGGVNSNRRLEDEEVEAPPPMPPPLVKQPPISSDHIGNNNQSHHHHHTMSNPNPQHNSAAWEYFFPSMEDIAGTSLNEEGQHGGGGVTFNRMQHTAMPSRVGIVEEPVTARMGVGVEIPGHIAEAEEVMESPMESPLPSGLKMKQMPVTPPSMEAKRIVKHNNSVNLVQIFTDLDDHFLKASESAHEVSKMLEATRLHYHSNFADNRGHIDHSARVMRVITWNRSFKGIPNLDDGKDDFDSDEHETHATILDKLLAWEKKLYDEVKAGELMKFDYQRKVASLNRLKKRGNNSEALEKAKAAVSQLHTRYIVDMQSLDSTVSEINRLRDEQLYPRLVKLVEEMASMWKKMLSEHEKQSETVTLLRSLDPSQSPKQTSEHHHERTYQLLVVVQQWHLQFEMLVNNQKGYIKSLSNWLKLNLIPIESSLKEKVSSPPRVRSPPVQGLLHAWHDRLEKLPDELARTAIGNFAAVIDTIFNQQEDEMILKRKCEESRKELSRKTRQFEDWYHKYMQRKMPEEVDPEAEDANAPDEIVTEKQFLVEQVRKRLEHEEAEYEKQCLQVRQKTLGSLKNRMPELFRAMCDFSLECSKMYMELGSISQHLSQNSS*