AlfalfaGEDB | Msa0225630

M. sativa cultivar zhongmu-4 / Msa0225630

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363
PFAM: PF13041.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0225630	XP_003597780.1	98.026	608	12	0	1	608	32	639	0.0	1234

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0225630	sp\|Q9LW84\|PP236_ARATH	69.130	609	185	2	1	608	35	641	0.0	900
Msa0225630	sp\|Q9LW84\|PP236_ARATH	26.199	271	196	3	95	363	374	642	1.42e-19	96.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0225630	G7II50	98.026	608	12	0	1	608	32	639	0.0	1234

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0185270	Msa0225630	0.881090	3.204251e-70	-8.615850e-47
Msa0225630	Msa0266480	0.880087	7.323329e-70	-8.615850e-47
Msa0225630	Msa0267760	0.805776	1.133523e-49	-8.615850e-47
Msa0225630	Msa0423360	-0.826085	3.291532e-54	-8.615850e-47
Msa0225630	Msa0526710	0.804455	2.142393e-49	-8.615850e-47
Msa0225630	Msa0546380	0.803863	2.845220e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0225630	MtrunA17_Chr2g0331911	98.026	608	12	0	1	608	32	639	0.0	1234
Msa0225630	MtrunA17_Chr5g0446031	28.499	593	384	7	44	600	83	671	5.69e-84	281
Msa0225630	MtrunA17_Chr5g0446031	29.764	467	325	3	135	601	384	847	5.74e-68	237
Msa0225630	MtrunA17_Chr5g0446031	28.921	491	336	7	95	578	413	897	9.57e-55	200
Msa0225630	MtrunA17_Chr4g0060641	28.277	534	380	3	64	596	10	541	2.12e-65	228
Msa0225630	MtrunA17_Chr4g0060641	27.579	475	339	4	125	596	210	682	1.31e-56	204
Msa0225630	MtrunA17_Chr4g0060641	23.944	497	306	5	182	607	22	517	1.87e-38	151
Msa0225630	MtrunA17_Chr4g0060641	28.571	378	230	4	90	430	317	691	4.98e-37	147
Msa0225630	MtrunA17_Chr4g0060641	25.234	214	160	0	93	306	495	708	3.06e-15	79.3
Msa0225630	MtrunA17_Chr1g0162741	27.521	585	385	7	46	596	26	605	3.25e-65	226

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0225630	AT3G16010.1	69.130	609	185	2	1	608	35	641	0.0	900
Msa0225630	AT3G16010.1	26.199	271	196	3	95	363	374	642	1.44e-20	96.3
Msa0225630	AT3G06920.2	26.355	664	401	9	10	599	35	684	3.36e-76	260
Msa0225630	AT3G06920.2	30.303	462	319	3	135	596	363	821	3.28e-69	241
Msa0225630	AT3G06920.2	28.571	406	285	3	135	540	432	832	1.41e-48	182
Msa0225630	AT3G06920.2	25.786	477	347	5	135	607	82	555	8.48e-47	177
Msa0225630	AT3G06920.2	26.509	381	245	3	93	439	495	874	5.04e-37	148
Msa0225630	AT3G06920.2	26.791	321	233	2	86	406	558	876	4.29e-28	120
Msa0225630	AT3G06920.3	26.355	664	401	9	10	599	35	684	3.36e-76	260
Msa0225630	AT3G06920.3	30.303	462	319	3	135	596	363	821	3.28e-69	241

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Find 137 sgRNAs with CRISPR-Local

Find 328 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGCTTTACTGCTTCTTG+AGG	0.580153	2_1:+75767840	Msa0225630:CDS
AAATTGTGAAAATCTTGGGT+AGG	0.544723	2_1:+75767062	Msa0225630:CDS
AACATTTCCATAGCACGTTT+CGG	0.304598	2_1:-75768255	None:intergenic
AACCATAACCTTCCAATGGA+AGG	0.556352	2_1:+75766692	Msa0225630:CDS
AACCATGAAGAAGCCTCAGA+AGG	0.602790	2_1:-75767730	None:intergenic
AATACTCTCCTTCCTTCCAT+TGG	0.452118	2_1:-75766704	None:intergenic
AATCTTGGGTAGGGTTAAGA+TGG	0.458908	2_1:+75767072	Msa0225630:CDS
AATGAGTATATACTTTAAGT+TGG	0.406232	2_1:+75767384	Msa0225630:CDS
AATGCGGCGGTGCGTCCCAA+CGG	0.567234	2_1:+75767447	Msa0225630:CDS
AATGGCTTGGCTAGGACCGG+TGG	0.626147	2_1:+75768231	Msa0225630:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATGCTAGAAAATTGGATGAA+AGG	+	chr2_1:75766741-75766760	Msa0225630:CDS	30.0%
AATTGGATGAAAGGTTCATT+AGG	+	chr2_1:75766750-75766769	Msa0225630:CDS	30.0%
ACATGATTCGACAACTTATA+TGG	+	chr2_1:75766934-75766953	Msa0225630:CDS	30.0%
TACGATGCTTAGATGAAAAT+AGG	+	chr2_1:75766963-75766982	Msa0225630:CDS	30.0%
ATGCTTAGATGAAAATAGGT+TGG	+	chr2_1:75766967-75766986	Msa0225630:CDS	30.0%
ACAATTTCAGACAATTCAGA+AGG	-	chr2_1:75767049-75767068	None:intergenic	30.0%
AAATTGTGAAAATCTTGGGT+AGG	+	chr2_1:75767062-75767081	Msa0225630:CDS	30.0%
AATTGTGAAAATCTTGGGTA+GGG	+	chr2_1:75767063-75767082	Msa0225630:CDS	30.0%
ACCCTTAACCTGATAAAAAA+TGG	-	chr2_1:75767113-75767132	None:intergenic	30.0%
GTATAATGAAATGTGTAGTG+AGG	+	chr2_1:75767219-75767238	Msa0225630:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	75766662	75768488	75766662	ID=Msa0225630;Name=Msa0225630
chr2_1	mRNA	75766662	75768488	75766662	ID=Msa0225630-mRNA-1;Parent=Msa0225630;Name=Msa0225630-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|608
chr2_1	exon	75766662	75768488	75766662	ID=Msa0225630-mRNA-1:exon:22724;Parent=Msa0225630-mRNA-1
chr2_1	CDS	75766662	75768488	75766662	ID=Msa0225630-mRNA-1:cds;Parent=Msa0225630-mRNA-1

Gene Sequence

>Msa0225630

ATGTTTCGGTTGCCTGATTCCCAAGAAGAAAACCATAACCTTCCAATGGAAGGAAGGAGAGTATTGAGGAAGGATCCAAATGCTAGAAAATTGGATGAAAGGTTCATTAGGATTTTGAAAATATTTAAATGGGGGCCTGATGCAGAAAAGGCTTTGGAAGTATTGAAGCTTAAGCTTGATATTCGCTTAGTTCGCGAAGTTTTGAAGATAGATGTTGAGGTCCATGTGAAGATTCAGTTTTTTAAGTGGGCTGGGAAGAAAAGGAATTTTGAACATGATTCGACAACTTATATGGCTTTGATACGATGCTTAGATGAAAATAGGTTGGTTGGTGAACTGTGGAGGACAATACAAGACATGGTTAAGAGTCCATGTGCGATTGGTCCTTCTGAATTGTCTGAAATTGTGAAAATCTTGGGTAGGGTTAAGATGGTTAACAAAGCATTATCCATTTTTTATCAGGTTAAGGGTCGCAAGTGCAGACCTACAGCAGGCACTTACAACTCTGTCATCTTGATGTTGATGCAAGAGGGGCATCATGAAAAAGTTCATGAGTTGTATAATGAAATGTGTAGTGAGGGACATTGTTTCCCTGATACAGTTACATATAGTGCACTTATATCAGCGTTTGGAAAACTAAATCGCGATGATTCTGCCGTCAGATTATTTAATGAGATGAAGGAGAATGGATTACAGCCAACAGCAAAAATTTATACAACTTTAATGAGTATATACTTTAAGTTGGGTAAGGTTGAGGAAGCGTTGAACCTGGTCCACGAGATGAGAATGCGGCGGTGCGTCCCAACGGTCTATACTTATACAGAATTGATAAGGGGATTGGGGAAATCCGGGAGGGTTGAAGACGCATGCGGAGTATACAAGAATATGCTGAAAGATGGTTGCAAACCAGATGTTGTCCTCATGAATAATTTGATTAACATTTTGGGTAGATCAGATCGCTTAAAAGAGGCTGTTGAGCTTTTTGAGGAAATGAATTTGCTTAATTGCACACCTAATGTTGTGACATACAACACCATTATCAAATCTCTATTTGAGGCTAAAGCCCCACCTTCTGAGGCTTCTTCATGGTTAGAGAGAATGAAAAAAGATGGAGTAGTTCCCAGCTCATTTACATATTCAATTCTCATCGATGGTTTCTGCAAAACAAATCGGGTTGAAAAGGCTTTACTGCTTCTTGAGGAGATGGATGAAAAAGGCTTTCCGCCTTGTCCTGCTGCCTACTGCAGCCTGATCAATAGCCTCGGTAAAGCAAAACGTTATGAGGCTGCAAATGAGCTGTTCCAAGAATTGAAAGAGAACTGTGGAAGTTCGAGCGCGCGTGTATATGCTGTCATGATTAAACATTTTGGAAAATGTGGACGATTCAATGAAGCTATGGGTCTTTTCAACGAGATGAAAAAACTTGGATGCATCCCCGATGTTTATGCTTATAATGCTCTCATGACCGGGATGGTAAGGGAAGACATGATGGATGAAGCTTTTTCTTTGTTTCGAACTATGGAAGAAAATGGTTGCAACCCAGATATAAATTCACATAACATCATACTAAATGGCTTGGCTAGGACCGGTGGCCCGAAACGTGCTATGGAAATGTTCGCGAAAATGAAGAGTTCTGCTATCAAGCCAGATGCGGTTTCTTACAACACAGTTCTTGGCTGCCTTAGTCGTGCAGGACTATTTGAAGAGGCTACTAAGCTTATGAAGGAAATGAGTTCAAAGGGATTCGAGTATGATCTAATCACCTACTCTTCAATTCTCGAGGCAGTTGGTAAGGTTGATGAAGATCGTAATATGGCAGAGTCATGA

Protein sequence

>Msa0225630

MFRLPDSQEENHNLPMEGRRVLRKDPNARKLDERFIRILKIFKWGPDAEKALEVLKLKLDIRLVREVLKIDVEVHVKIQFFKWAGKKRNFEHDSTTYMALIRCLDENRLVGELWRTIQDMVKSPCAIGPSELSEIVKILGRVKMVNKALSIFYQVKGRKCRPTAGTYNSVILMLMQEGHHEKVHELYNEMCSEGHCFPDTVTYSALISAFGKLNRDDSAVRLFNEMKENGLQPTAKIYTTLMSIYFKLGKVEEALNLVHEMRMRRCVPTVYTYTELIRGLGKSGRVEDACGVYKNMLKDGCKPDVVLMNNLINILGRSDRLKEAVELFEEMNLLNCTPNVVTYNTIIKSLFEAKAPPSEASSWLERMKKDGVVPSSFTYSILIDGFCKTNRVEKALLLLEEMDEKGFPPCPAAYCSLINSLGKAKRYEAANELFQELKENCGSSSARVYAVMIKHFGKCGRFNEAMGLFNEMKKLGCIPDVYAYNALMTGMVREDMMDEAFSLFRTMEENGCNPDINSHNIILNGLARTGGPKRAMEMFAKMKSSAIKPDAVSYNTVLGCLSRAGLFEEATKLMKEMSSKGFEYDLITYSSILEAVGKVDEDRNMAES*