AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0227260

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0227260 MtrunA17_Chr2g0325301 99.291 141 1 0 6 146 8 148 5.25e-101 286
Msa0227260 MtrunA17_Chr6g0451421 58.696 138 57 0 6 143 6 143 1.24e-53 166
Msa0227260 MtrunA17_Chr6g0451411 59.559 136 55 0 8 143 176 311 4.85e-52 167
Msa0227260 MtrunA17_Chr2g0329701 51.429 140 68 0 7 146 233 372 6.36e-46 153
Msa0227260 MtrunA17_Chr7g0264421 51.825 137 66 0 7 143 203 339 1.17e-45 151
Msa0227260 MtrunA17_Chr6g0451441 46.575 146 70 1 6 143 318 463 1.54e-35 127
Msa0227260 MtrunA17_Chr6g0451451 50.000 82 41 0 62 143 1 82 5.83e-25 91.3
Msa0227260 MtrunA17_Chr1g0158081 37.190 121 74 1 11 131 1209 1327 3.24e-16 74.3
Msa0227260 MtrunA17_Chr3g0110591 36.364 121 75 1 11 131 25 143 3.66e-15 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0227260 AT3G01410.2 50.000 136 68 0 8 143 154 289 3.37e-45 149
Msa0227260 AT3G01410.1 50.000 136 68 0 8 143 154 289 3.37e-45 149
Msa0227260 AT5G51080.3 53.285 137 64 0 7 143 121 257 4.79e-45 148
Msa0227260 AT5G51080.1 53.676 136 63 0 8 143 185 320 2.57e-44 148
Msa0227260 AT5G51080.2 53.676 136 63 0 8 143 185 320 2.57e-44 148
Msa0227260 AT1G24090.1 52.143 140 67 0 4 143 212 351 1.92e-41 141

Find 50 sgRNAs with CRISPR-Local

Find 229 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACAAAGGTTCCTTAAATT+TGG 0.088756 2_1:-78056878 None:intergenic
TGCCTACTAGTTCAGCATTT+TGG 0.102415 2_1:+78057699 Msa0227260:three_prime_UTR
TGAATATAATGGTCTAATTT+TGG 0.114690 2_1:+78056323 Msa0227260:CDS
ATTGCTTAAGTGACTTAAAA+TGG 0.233393 2_1:+78057810 Msa0227260:three_prime_UTR
TTCTATCTTCTGTCTTCATT+TGG 0.279448 2_1:+78057887 Msa0227260:three_prime_UTR
TACAAAGGTTCCTTAAATTT+GGG 0.313196 2_1:-78056877 None:intergenic
TGCAACAGTTTGAGGGTTCC+TGG 0.323750 2_1:+78056843 Msa0227260:intron
TGGATGCTCCATCAAATTCT+AGG 0.329321 2_1:-78056110 None:intergenic
AAGTCAACAACCCAAATTTA+AGG 0.331211 2_1:+78056867 Msa0227260:CDS
ATTTGGGTTGTTGACTTTCC+AGG 0.336100 2_1:-78056861 None:intergenic
AATGGTGTTGCTTTGATGTT+TGG 0.346803 2_1:+78057828 Msa0227260:three_prime_UTR
TCTTGTATCCTAGAATTTGA+TGG 0.357172 2_1:+78056102 Msa0227260:CDS
CTAGTAGGCACAACCTAAAC+AGG 0.385949 2_1:-78057686 None:intergenic
AATAATTCTGCTGAATATAA+TGG 0.390357 2_1:+78056312 Msa0227260:CDS
GCTCCTGCACCAGACCTTCC+AGG 0.402463 2_1:-78056141 None:intergenic
TGCGTTCTGCTGATGGGAGT+CGG 0.407658 2_1:+78056169 Msa0227260:CDS
CTCTCAGCTTGTTTGCAAGC+AGG 0.424209 2_1:+78056398 Msa0227260:CDS
TTTAAGGAACCTTTGTAATG+AGG 0.445867 2_1:+78056883 Msa0227260:CDS
GCTGTTCTGCGTTCTGCTGA+TGG 0.448826 2_1:+78056162 Msa0227260:CDS
GGAGCATCCAGTGGAAATCC+TGG 0.459158 2_1:+78056123 Msa0227260:CDS
GCGTTCTGCTGATGGGAGTC+GGG 0.465577 2_1:+78056170 Msa0227260:CDS
AGTGGAAATCCTGGAAGGTC+TGG 0.475520 2_1:+78056132 Msa0227260:CDS
CTGTTCTGCGTTCTGCTGAT+GGG 0.478692 2_1:+78056163 Msa0227260:CDS
TATGTCTCTTTATAGGTCCA+TGG 0.481724 2_1:+78056261 Msa0227260:intron
TTGTTTGAGTGCCGAGTCCT+TGG 0.487817 2_1:-78056290 None:intergenic
TCAGTGTGGAACATGTTCCT+AGG 0.499661 2_1:+78056936 Msa0227260:CDS
CATGAACATGCTCATATCCT+TGG 0.505728 2_1:-78056365 None:intergenic
CATGGCGTCAGCCAAGGACT+CGG 0.511067 2_1:+78056279 Msa0227260:CDS
TATGACATGGCCAACATGAC+TGG 0.542678 2_1:+78057937 Msa0227260:three_prime_UTR
CGAGTCCTTGGCTGACGCCA+TGG 0.551131 2_1:-78056278 None:intergenic
AGACCTTCCAGGATTTCCAC+TGG 0.556723 2_1:-78056130 None:intergenic
TAGGTCCATGGCGTCAGCCA+AGG 0.557191 2_1:+78056273 Msa0227260:intron
GAACCTTTGTAATGAGGCTG+TGG 0.560005 2_1:+78056889 Msa0227260:CDS
CCCCAAAATGCTGAACTAGT+AGG 0.561322 2_1:-78057701 None:intergenic
AATCCTGGAAGGTCTGGTGC+AGG 0.565510 2_1:+78056138 Msa0227260:CDS
CAGTGTGGAACATGTTCCTA+GGG 0.573649 2_1:+78056937 Msa0227260:CDS
GTAGTAATATTCAACTTCGC+CGG 0.574495 2_1:-78057604 None:intergenic
ATGGTGAGATACACACATCA+TGG 0.603252 2_1:-78057789 None:intergenic
GCTCCACAGCCTCATTACAA+AGG 0.619284 2_1:-78056892 None:intergenic
TTAACACCATCCAGTCATGT+TGG 0.629124 2_1:-78057947 None:intergenic
CTGAATGAAGCTAGGAACCA+AGG 0.629738 2_1:+78056348 Msa0227260:CDS
GAATTTGATGGAGCATCCAG+TGG 0.630666 2_1:+78056114 Msa0227260:CDS
TATGAGCATGTTCATGTCCG+AGG 0.645245 2_1:+78056372 Msa0227260:CDS
GCTGATGCTCAAGCAAATCG+CGG 0.646058 2_1:+78057321 Msa0227260:CDS
ACATGGCCAACATGACTGGA+TGG 0.655888 2_1:+78057941 Msa0227260:three_prime_UTR
CATCCAGTGGAAATCCTGGA+AGG 0.657104 2_1:+78056127 Msa0227260:CDS
TAACTTCAAGTCAGTCAGTG+TGG 0.658191 2_1:+78056922 Msa0227260:CDS
TAATATATTGTGCTATGACA+TGG 0.692388 2_1:+78057924 Msa0227260:three_prime_UTR
AAACAAGCTGAGAGTCACCT+CGG 0.700596 2_1:-78056389 None:intergenic
GAAATTCAGAAACATTCACA+TGG 0.721736 2_1:-78057867 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAATATTTTGTGAACAAT+CGG - chr2_1:78056585-78056604 None:intergenic 15.0%
!!! ATTTACCATCATATTTTTAT+TGG + chr2_1:78056817-78056836 Msa0227260:intron 15.0%
!!! TTTTTATATTTGTATTTGGA+AGG + chr2_1:78057522-78057541 Msa0227260:intron 15.0%
!!! TTTTATATTTGTATTTGGAA+GGG + chr2_1:78057523-78057542 Msa0227260:intron 15.0%
!! AACAAAACAAAACAAAATAT+AGG - chr2_1:78057569-78057588 None:intergenic 15.0%
!!! AATAATATTTTGTGAACAAT+CGG - chr2_1:78056585-78056604 None:intergenic 15.0%
!!! ATTTACCATCATATTTTTAT+TGG + chr2_1:78056817-78056836 Msa0227260:intron 15.0%
!!! TTTTTATATTTGTATTTGGA+AGG + chr2_1:78057522-78057541 Msa0227260:intron 15.0%
!!! TTTTATATTTGTATTTGGAA+GGG + chr2_1:78057523-78057542 Msa0227260:intron 15.0%
!! AACAAAACAAAACAAAATAT+AGG - chr2_1:78057569-78057588 None:intergenic 15.0%
!! AATAATTCTGCTGAATATAA+TGG + chr2_1:78056312-78056331 Msa0227260:CDS 20.0%
!!! TGAATATAATGGTCTAATTT+TGG + chr2_1:78056323-78056342 Msa0227260:CDS 20.0%
!!! GAATATAATGGTCTAATTTT+GGG + chr2_1:78056324-78056343 Msa0227260:CDS 20.0%
!!! AATATAATGGTCTAATTTTG+GGG + chr2_1:78056325-78056344 Msa0227260:CDS 20.0%
!! TGTTCACAAAATATTATTAC+AGG + chr2_1:78056587-78056606 Msa0227260:intron 20.0%
!! ATGTTGTAATTATTACTACA+TGG + chr2_1:78056688-78056707 Msa0227260:intron 20.0%
!! CAAAACCAATAAAAATATGA+TGG - chr2_1:78056825-78056844 None:intergenic 20.0%
!! CATAAACAATTCTTAAATGT+TGG - chr2_1:78057094-78057113 None:intergenic 20.0%
!! TTTATATTTGTATTTGGAAG+GGG + chr2_1:78057524-78057543 Msa0227260:intron 20.0%
!! AATAATTCTGCTGAATATAA+TGG + chr2_1:78056312-78056331 Msa0227260:CDS 20.0%
!!! TGAATATAATGGTCTAATTT+TGG + chr2_1:78056323-78056342 Msa0227260:CDS 20.0%
!!! GAATATAATGGTCTAATTTT+GGG + chr2_1:78056324-78056343 Msa0227260:CDS 20.0%
!!! AATATAATGGTCTAATTTTG+GGG + chr2_1:78056325-78056344 Msa0227260:CDS 20.0%
!! TGTTCACAAAATATTATTAC+AGG + chr2_1:78056587-78056606 Msa0227260:intron 20.0%
!! ATGTTGTAATTATTACTACA+TGG + chr2_1:78056688-78056707 Msa0227260:intron 20.0%
!! CAAAACCAATAAAAATATGA+TGG - chr2_1:78056825-78056844 None:intergenic 20.0%
!! CATAAACAATTCTTAAATGT+TGG - chr2_1:78057094-78057113 None:intergenic 20.0%
!! TTTATATTTGTATTTGGAAG+GGG + chr2_1:78057524-78057543 Msa0227260:intron 20.0%
! ATAGTGCAAAAATGATTTGA+TGG - chr2_1:78056208-78056227 None:intergenic 25.0%
! AACACTTTATGTCTCTTTAT+AGG + chr2_1:78056254-78056273 Msa0227260:intron 25.0%
! ATGAGTTATAATAGACGTAA+GGG - chr2_1:78056430-78056449 None:intergenic 25.0%
!! AAATCACCGTGTAATATTTT+GGG - chr2_1:78056665-78056684 None:intergenic 25.0%
! ACACTATATATCAAGCAATT+AGG - chr2_1:78056760-78056779 None:intergenic 25.0%
! TATAGTGTTCCTATAGTAAT+TGG + chr2_1:78056772-78056791 Msa0227260:intron 25.0%
! CAAATCAAACCAATTACTAT+AGG - chr2_1:78056784-78056803 None:intergenic 25.0%
! TACAAAGGTTCCTTAAATTT+GGG - chr2_1:78056880-78056899 None:intergenic 25.0%
! TTACAAAGGTTCCTTAAATT+TGG - chr2_1:78056881-78056900 None:intergenic 25.0%
!!! TCATGTTTTTGTATTGTTGA+AGG + chr2_1:78057284-78057303 Msa0227260:intron 25.0%
!!! CATGTTTTTGTATTGTTGAA+GGG + chr2_1:78057285-78057304 Msa0227260:intron 25.0%
! AAACATCTTTCACTTGTATT+TGG + chr2_1:78057456-78057475 Msa0227260:intron 25.0%
!!! TGTTTTTTTTTTTTTGCAGC+CGG + chr2_1:78057585-78057604 Msa0227260:intron 25.0%
!! CTATGTTAATTTTCCTGTTT+AGG + chr2_1:78057673-78057692 Msa0227260:three_prime_UTR 25.0%
!!! ATTTTAAGTCACTTAAGCAA+TGG - chr2_1:78057811-78057830 None:intergenic 25.0%
! ATTGCTTAAGTGACTTAAAA+TGG + chr2_1:78057810-78057829 Msa0227260:three_prime_UTR 25.0%
! TAATATATTGTGCTATGACA+TGG + chr2_1:78057924-78057943 Msa0227260:three_prime_UTR 25.0%
! ATAGTGCAAAAATGATTTGA+TGG - chr2_1:78056208-78056227 None:intergenic 25.0%
! AACACTTTATGTCTCTTTAT+AGG + chr2_1:78056254-78056273 Msa0227260:intron 25.0%
! ATGAGTTATAATAGACGTAA+GGG - chr2_1:78056430-78056449 None:intergenic 25.0%
!! AAATCACCGTGTAATATTTT+GGG - chr2_1:78056665-78056684 None:intergenic 25.0%
! ACACTATATATCAAGCAATT+AGG - chr2_1:78056760-78056779 None:intergenic 25.0%
! TATAGTGTTCCTATAGTAAT+TGG + chr2_1:78056772-78056791 Msa0227260:intron 25.0%
! CAAATCAAACCAATTACTAT+AGG - chr2_1:78056784-78056803 None:intergenic 25.0%
! TACAAAGGTTCCTTAAATTT+GGG - chr2_1:78056880-78056899 None:intergenic 25.0%
! TTACAAAGGTTCCTTAAATT+TGG - chr2_1:78056881-78056900 None:intergenic 25.0%
!!! TCATGTTTTTGTATTGTTGA+AGG + chr2_1:78057284-78057303 Msa0227260:intron 25.0%
!!! CATGTTTTTGTATTGTTGAA+GGG + chr2_1:78057285-78057304 Msa0227260:intron 25.0%
! AAACATCTTTCACTTGTATT+TGG + chr2_1:78057456-78057475 Msa0227260:intron 25.0%
!!! TGTTTTTTTTTTTTTGCAGC+CGG + chr2_1:78057585-78057604 Msa0227260:intron 25.0%
!! CTATGTTAATTTTCCTGTTT+AGG + chr2_1:78057673-78057692 Msa0227260:three_prime_UTR 25.0%
!!! ATTTTAAGTCACTTAAGCAA+TGG - chr2_1:78057811-78057830 None:intergenic 25.0%
! ATTGCTTAAGTGACTTAAAA+TGG + chr2_1:78057810-78057829 Msa0227260:three_prime_UTR 25.0%
! TAATATATTGTGCTATGACA+TGG + chr2_1:78057924-78057943 Msa0227260:three_prime_UTR 25.0%
TCTTGTATCCTAGAATTTGA+TGG + chr2_1:78056102-78056121 Msa0227260:CDS 30.0%
GATGAGTTATAATAGACGTA+AGG - chr2_1:78056431-78056450 None:intergenic 30.0%
TGTTAATGCATACACTGTAA+GGG - chr2_1:78056486-78056505 None:intergenic 30.0%
ATGTTAATGCATACACTGTA+AGG - chr2_1:78056487-78056506 None:intergenic 30.0%
CATCACTCATTTCATTCATA+TGG - chr2_1:78056544-78056563 None:intergenic 30.0%
TTGGTACCCAAAATATTACA+CGG + chr2_1:78056656-78056675 Msa0227260:intron 30.0%
! CAAATCACCGTGTAATATTT+TGG - chr2_1:78056666-78056685 None:intergenic 30.0%
TATATATCAAGCAATTAGGC+AGG - chr2_1:78056756-78056775 None:intergenic 30.0%
AAGTCAACAACCCAAATTTA+AGG + chr2_1:78056867-78056886 Msa0227260:CDS 30.0%
TTTAAGGAACCTTTGTAATG+AGG + chr2_1:78056883-78056902 Msa0227260:CDS 30.0%
! GTTTCTCTGGAAAACTTAAA+AGG + chr2_1:78057044-78057063 Msa0227260:intron 30.0%
! GTTTTTCCTGTTAAAGCATA+TGG - chr2_1:78057175-78057194 None:intergenic 30.0%
!!! CAAGACTTTTGAGTTCTTAT+AGG + chr2_1:78057369-78057388 Msa0227260:intron 30.0%
!!! ATGTTTTTCTAAAGCAGCTT+TGG - chr2_1:78057442-78057461 None:intergenic 30.0%
! TGATGTTTGGATTTTATGCT+TGG + chr2_1:78057841-78057860 Msa0227260:three_prime_UTR 30.0%
GAAATTCAGAAACATTCACA+TGG - chr2_1:78057870-78057889 None:intergenic 30.0%
TTCTATCTTCTGTCTTCATT+TGG + chr2_1:78057887-78057906 Msa0227260:three_prime_UTR 30.0%
! TGTTCATGTCATTTCATAGA+TGG - chr2_1:78056068-78056087 None:intergenic 30.0%
TCTTGTATCCTAGAATTTGA+TGG + chr2_1:78056102-78056121 Msa0227260:CDS 30.0%
GATGAGTTATAATAGACGTA+AGG - chr2_1:78056431-78056450 None:intergenic 30.0%
TGTTAATGCATACACTGTAA+GGG - chr2_1:78056486-78056505 None:intergenic 30.0%
ATGTTAATGCATACACTGTA+AGG - chr2_1:78056487-78056506 None:intergenic 30.0%
CATCACTCATTTCATTCATA+TGG - chr2_1:78056544-78056563 None:intergenic 30.0%
TTGGTACCCAAAATATTACA+CGG + chr2_1:78056656-78056675 Msa0227260:intron 30.0%
! CAAATCACCGTGTAATATTT+TGG - chr2_1:78056666-78056685 None:intergenic 30.0%
TATATATCAAGCAATTAGGC+AGG - chr2_1:78056756-78056775 None:intergenic 30.0%
AAGTCAACAACCCAAATTTA+AGG + chr2_1:78056867-78056886 Msa0227260:CDS 30.0%
TTTAAGGAACCTTTGTAATG+AGG + chr2_1:78056883-78056902 Msa0227260:CDS 30.0%
! GTTTCTCTGGAAAACTTAAA+AGG + chr2_1:78057044-78057063 Msa0227260:intron 30.0%
! GTTTTTCCTGTTAAAGCATA+TGG - chr2_1:78057175-78057194 None:intergenic 30.0%
!!! CAAGACTTTTGAGTTCTTAT+AGG + chr2_1:78057369-78057388 Msa0227260:intron 30.0%
!!! ATGTTTTTCTAAAGCAGCTT+TGG - chr2_1:78057442-78057461 None:intergenic 30.0%
! TGATGTTTGGATTTTATGCT+TGG + chr2_1:78057841-78057860 Msa0227260:three_prime_UTR 30.0%
GAAATTCAGAAACATTCACA+TGG - chr2_1:78057870-78057889 None:intergenic 30.0%
TTCTATCTTCTGTCTTCATT+TGG + chr2_1:78057887-78057906 Msa0227260:three_prime_UTR 30.0%
TATGTCTCTTTATAGGTCCA+TGG + chr2_1:78056261-78056280 Msa0227260:intron 35.0%
! TAAATTCCTTGTTTGCAAGC+AGG + chr2_1:78056454-78056473 Msa0227260:intron 35.0%
!! GTGTAATATTTTGGGTACCA+AGG - chr2_1:78056657-78056676 None:intergenic 35.0%
!!! ATTGGTTTTGCAACAGTTTG+AGG + chr2_1:78056835-78056854 Msa0227260:intron 35.0%
!!! TTGGTTTTGCAACAGTTTGA+GGG + chr2_1:78056836-78056855 Msa0227260:intron 35.0%
GGTATACACTTTGTCTCATT+TGG + chr2_1:78056958-78056977 Msa0227260:intron 35.0%
AGAGAAACACACAATCAAGA+AGG - chr2_1:78057032-78057051 None:intergenic 35.0%
TTCTTGATTGTGTGTTTCTC+TGG + chr2_1:78057031-78057050 Msa0227260:intron 35.0%
CTGGAAAACTTAAAAGGTTG+AGG + chr2_1:78057050-78057069 Msa0227260:intron 35.0%
! GAGAGGCATGCTAATTAAAA+AGG - chr2_1:78057135-78057154 None:intergenic 35.0%
TCACTTGTATTTGGTTGCAA+TGG + chr2_1:78057465-78057484 Msa0227260:intron 35.0%
GTAGTAATATTCAACTTCGC+CGG - chr2_1:78057607-78057626 None:intergenic 35.0%
ATGCCACTGTCAAAAACTAT+TGG + chr2_1:78057647-78057666 Msa0227260:three_prime_UTR 35.0%
! TTGCCAATAGTTTTTGACAG+TGG - chr2_1:78057653-78057672 None:intergenic 35.0%
!! AATGGTGTTGCTTTGATGTT+TGG + chr2_1:78057828-78057847 Msa0227260:three_prime_UTR 35.0%
TATGTCTCTTTATAGGTCCA+TGG + chr2_1:78056261-78056280 Msa0227260:intron 35.0%
! TAAATTCCTTGTTTGCAAGC+AGG + chr2_1:78056454-78056473 Msa0227260:intron 35.0%
!! GTGTAATATTTTGGGTACCA+AGG - chr2_1:78056657-78056676 None:intergenic 35.0%
!!! ATTGGTTTTGCAACAGTTTG+AGG + chr2_1:78056835-78056854 Msa0227260:intron 35.0%
!!! TTGGTTTTGCAACAGTTTGA+GGG + chr2_1:78056836-78056855 Msa0227260:intron 35.0%
GGTATACACTTTGTCTCATT+TGG + chr2_1:78056958-78056977 Msa0227260:intron 35.0%
AGAGAAACACACAATCAAGA+AGG - chr2_1:78057032-78057051 None:intergenic 35.0%
TTCTTGATTGTGTGTTTCTC+TGG + chr2_1:78057031-78057050 Msa0227260:intron 35.0%
CTGGAAAACTTAAAAGGTTG+AGG + chr2_1:78057050-78057069 Msa0227260:intron 35.0%
! GAGAGGCATGCTAATTAAAA+AGG - chr2_1:78057135-78057154 None:intergenic 35.0%
TCACTTGTATTTGGTTGCAA+TGG + chr2_1:78057465-78057484 Msa0227260:intron 35.0%
GTAGTAATATTCAACTTCGC+CGG - chr2_1:78057607-78057626 None:intergenic 35.0%
ATGCCACTGTCAAAAACTAT+TGG + chr2_1:78057647-78057666 Msa0227260:three_prime_UTR 35.0%
! TTGCCAATAGTTTTTGACAG+TGG - chr2_1:78057653-78057672 None:intergenic 35.0%
!! AATGGTGTTGCTTTGATGTT+TGG + chr2_1:78057828-78057847 Msa0227260:three_prime_UTR 35.0%
TGGATGCTCCATCAAATTCT+AGG - chr2_1:78056113-78056132 None:intergenic 40.0%
CATGAACATGCTCATATCCT+TGG - chr2_1:78056368-78056387 None:intergenic 40.0%
AACTAACCTGCTTGCAAACA+AGG - chr2_1:78056407-78056426 None:intergenic 40.0%
TCTGCATTAACAGCAATGCA+AGG + chr2_1:78056713-78056732 Msa0227260:intron 40.0%
!! ATTTGGGTTGTTGACTTTCC+AGG - chr2_1:78056864-78056883 None:intergenic 40.0%
TAACTTCAAGTCAGTCAGTG+TGG + chr2_1:78056922-78056941 Msa0227260:CDS 40.0%
TGAGACAAAGTGTATACCCT+AGG - chr2_1:78056956-78056975 None:intergenic 40.0%
! TAGTTTTTGACAGTGGCATG+CGG - chr2_1:78057646-78057665 None:intergenic 40.0%
! TGCCTACTAGTTCAGCATTT+TGG + chr2_1:78057699-78057718 Msa0227260:three_prime_UTR 40.0%
! GCCTACTAGTTCAGCATTTT+GGG + chr2_1:78057700-78057719 Msa0227260:three_prime_UTR 40.0%
! CCTACTAGTTCAGCATTTTG+GGG + chr2_1:78057701-78057720 Msa0227260:three_prime_UTR 40.0%
! CTACTAGTTCAGCATTTTGG+GGG + chr2_1:78057702-78057721 Msa0227260:three_prime_UTR 40.0%
ATGGTGAGATACACACATCA+TGG - chr2_1:78057792-78057811 None:intergenic 40.0%
TTAACACCATCCAGTCATGT+TGG - chr2_1:78057950-78057969 None:intergenic 40.0%
TGGATGCTCCATCAAATTCT+AGG - chr2_1:78056113-78056132 None:intergenic 40.0%
CATGAACATGCTCATATCCT+TGG - chr2_1:78056368-78056387 None:intergenic 40.0%
AACTAACCTGCTTGCAAACA+AGG - chr2_1:78056407-78056426 None:intergenic 40.0%
TCTGCATTAACAGCAATGCA+AGG + chr2_1:78056713-78056732 Msa0227260:intron 40.0%
!! ATTTGGGTTGTTGACTTTCC+AGG - chr2_1:78056864-78056883 None:intergenic 40.0%
TAACTTCAAGTCAGTCAGTG+TGG + chr2_1:78056922-78056941 Msa0227260:CDS 40.0%
TGAGACAAAGTGTATACCCT+AGG - chr2_1:78056956-78056975 None:intergenic 40.0%
! TAGTTTTTGACAGTGGCATG+CGG - chr2_1:78057646-78057665 None:intergenic 40.0%
! TGCCTACTAGTTCAGCATTT+TGG + chr2_1:78057699-78057718 Msa0227260:three_prime_UTR 40.0%
! GCCTACTAGTTCAGCATTTT+GGG + chr2_1:78057700-78057719 Msa0227260:three_prime_UTR 40.0%
! CCTACTAGTTCAGCATTTTG+GGG + chr2_1:78057701-78057720 Msa0227260:three_prime_UTR 40.0%
! CTACTAGTTCAGCATTTTGG+GGG + chr2_1:78057702-78057721 Msa0227260:three_prime_UTR 40.0%
ATGGTGAGATACACACATCA+TGG - chr2_1:78057792-78057811 None:intergenic 40.0%
TTAACACCATCCAGTCATGT+TGG - chr2_1:78057950-78057969 None:intergenic 40.0%
! GAATTTGATGGAGCATCCAG+TGG + chr2_1:78056114-78056133 Msa0227260:CDS 45.0%
!!! TTTTGGGGCTGAATGAAGCT+AGG + chr2_1:78056340-78056359 Msa0227260:CDS 45.0%
CTGAATGAAGCTAGGAACCA+AGG + chr2_1:78056348-78056367 Msa0227260:CDS 45.0%
TATGAGCATGTTCATGTCCG+AGG + chr2_1:78056372-78056391 Msa0227260:CDS 45.0%
AAACAAGCTGAGAGTCACCT+CGG - chr2_1:78056392-78056411 None:intergenic 45.0%
GAACCTTTGTAATGAGGCTG+TGG + chr2_1:78056889-78056908 Msa0227260:CDS 45.0%
TCAGTGTGGAACATGTTCCT+AGG + chr2_1:78056936-78056955 Msa0227260:CDS 45.0%
CAGTGTGGAACATGTTCCTA+GGG + chr2_1:78056937-78056956 Msa0227260:CDS 45.0%
ATCGCGTGACTTATGTAGAG+AGG - chr2_1:78057152-78057171 None:intergenic 45.0%
CGCGATCCATATGCTTTAAC+AGG + chr2_1:78057166-78057185 Msa0227260:intron 45.0%
GCAAATCGCGGCAAAAATCT+TGG + chr2_1:78057333-78057352 Msa0227260:CDS 45.0%
AATCGCGGCAAAAATCTTGG+AGG + chr2_1:78057336-78057355 Msa0227260:CDS 45.0%
CTAGTAGGCACAACCTAAAC+AGG - chr2_1:78057689-78057708 None:intergenic 45.0%
CCCCAAAATGCTGAACTAGT+AGG - chr2_1:78057704-78057723 None:intergenic 45.0%
TATGACATGGCCAACATGAC+TGG + chr2_1:78057937-78057956 Msa0227260:three_prime_UTR 45.0%
! GAATTTGATGGAGCATCCAG+TGG + chr2_1:78056114-78056133 Msa0227260:CDS 45.0%
!!! TTTTGGGGCTGAATGAAGCT+AGG + chr2_1:78056340-78056359 Msa0227260:CDS 45.0%
CTGAATGAAGCTAGGAACCA+AGG + chr2_1:78056348-78056367 Msa0227260:CDS 45.0%
TATGAGCATGTTCATGTCCG+AGG + chr2_1:78056372-78056391 Msa0227260:CDS 45.0%
AAACAAGCTGAGAGTCACCT+CGG - chr2_1:78056392-78056411 None:intergenic 45.0%
GAACCTTTGTAATGAGGCTG+TGG + chr2_1:78056889-78056908 Msa0227260:CDS 45.0%
TCAGTGTGGAACATGTTCCT+AGG + chr2_1:78056936-78056955 Msa0227260:CDS 45.0%
CAGTGTGGAACATGTTCCTA+GGG + chr2_1:78056937-78056956 Msa0227260:CDS 45.0%
ATCGCGTGACTTATGTAGAG+AGG - chr2_1:78057152-78057171 None:intergenic 45.0%
CGCGATCCATATGCTTTAAC+AGG + chr2_1:78057166-78057185 Msa0227260:intron 45.0%
GCAAATCGCGGCAAAAATCT+TGG + chr2_1:78057333-78057352 Msa0227260:CDS 45.0%
AATCGCGGCAAAAATCTTGG+AGG + chr2_1:78057336-78057355 Msa0227260:CDS 45.0%
CTAGTAGGCACAACCTAAAC+AGG - chr2_1:78057689-78057708 None:intergenic 45.0%
CCCCAAAATGCTGAACTAGT+AGG - chr2_1:78057704-78057723 None:intergenic 45.0%
TATGACATGGCCAACATGAC+TGG + chr2_1:78057937-78057956 Msa0227260:three_prime_UTR 45.0%
!!! ATTTTTTTTATATTTGTATT+TGG + chr2_1:78057518-78057537 Msa0227260:intron 5.0%
!!! ATTTTTTTTATATTTGTATT+TGG + chr2_1:78057518-78057537 Msa0227260:intron 5.0%
CATCCAGTGGAAATCCTGGA+AGG + chr2_1:78056127-78056146 Msa0227260:CDS 50.0%
AGACCTTCCAGGATTTCCAC+TGG - chr2_1:78056133-78056152 None:intergenic 50.0%
AGTGGAAATCCTGGAAGGTC+TGG + chr2_1:78056132-78056151 Msa0227260:CDS 50.0%
CTGTTCTGCGTTCTGCTGAT+GGG + chr2_1:78056163-78056182 Msa0227260:CDS 50.0%
!! TTGTTTGAGTGCCGAGTCCT+TGG - chr2_1:78056293-78056312 None:intergenic 50.0%
! CTCTCAGCTTGTTTGCAAGC+AGG + chr2_1:78056398-78056417 Msa0227260:CDS 50.0%
!! GTTTTCCTATGCGTCGACCT+TGG + chr2_1:78056637-78056656 Msa0227260:intron 50.0%
TGCAACAGTTTGAGGGTTCC+TGG + chr2_1:78056843-78056862 Msa0227260:intron 50.0%
GCTCCACAGCCTCATTACAA+AGG - chr2_1:78056895-78056914 None:intergenic 50.0%
GCTGATGCTCAAGCAAATCG+CGG + chr2_1:78057321-78057340 Msa0227260:CDS 50.0%
ACATGGCCAACATGACTGGA+TGG + chr2_1:78057941-78057960 Msa0227260:three_prime_UTR 50.0%
CATCCAGTGGAAATCCTGGA+AGG + chr2_1:78056127-78056146 Msa0227260:CDS 50.0%
AGACCTTCCAGGATTTCCAC+TGG - chr2_1:78056133-78056152 None:intergenic 50.0%
AGTGGAAATCCTGGAAGGTC+TGG + chr2_1:78056132-78056151 Msa0227260:CDS 50.0%
CTGTTCTGCGTTCTGCTGAT+GGG + chr2_1:78056163-78056182 Msa0227260:CDS 50.0%
!! TTGTTTGAGTGCCGAGTCCT+TGG - chr2_1:78056293-78056312 None:intergenic 50.0%
! CTCTCAGCTTGTTTGCAAGC+AGG + chr2_1:78056398-78056417 Msa0227260:CDS 50.0%
!! GTTTTCCTATGCGTCGACCT+TGG + chr2_1:78056637-78056656 Msa0227260:intron 50.0%
TGCAACAGTTTGAGGGTTCC+TGG + chr2_1:78056843-78056862 Msa0227260:intron 50.0%
GCTCCACAGCCTCATTACAA+AGG - chr2_1:78056895-78056914 None:intergenic 50.0%
GCTGATGCTCAAGCAAATCG+CGG + chr2_1:78057321-78057340 Msa0227260:CDS 50.0%
ACATGGCCAACATGACTGGA+TGG + chr2_1:78057941-78057960 Msa0227260:three_prime_UTR 50.0%
! GGAGCATCCAGTGGAAATCC+TGG + chr2_1:78056123-78056142 Msa0227260:CDS 55.0%
AATCCTGGAAGGTCTGGTGC+AGG + chr2_1:78056138-78056157 Msa0227260:CDS 55.0%
GCTGTTCTGCGTTCTGCTGA+TGG + chr2_1:78056162-78056181 Msa0227260:CDS 55.0%
TGCGTTCTGCTGATGGGAGT+CGG + chr2_1:78056169-78056188 Msa0227260:CDS 55.0%
! GGAGCATCCAGTGGAAATCC+TGG + chr2_1:78056123-78056142 Msa0227260:CDS 55.0%
AATCCTGGAAGGTCTGGTGC+AGG + chr2_1:78056138-78056157 Msa0227260:CDS 55.0%
GCTGTTCTGCGTTCTGCTGA+TGG + chr2_1:78056162-78056181 Msa0227260:CDS 55.0%
TGCGTTCTGCTGATGGGAGT+CGG + chr2_1:78056169-78056188 Msa0227260:CDS 55.0%
GCGTTCTGCTGATGGGAGTC+GGG + chr2_1:78056170-78056189 Msa0227260:CDS 60.0%
TAGGTCCATGGCGTCAGCCA+AGG + chr2_1:78056273-78056292 Msa0227260:intron 60.0%
CATGGCGTCAGCCAAGGACT+CGG + chr2_1:78056279-78056298 Msa0227260:CDS 60.0%
GGGTACCAAGGTCGACGCAT+AGG - chr2_1:78056645-78056664 None:intergenic 60.0%
GCGTTCTGCTGATGGGAGTC+GGG + chr2_1:78056170-78056189 Msa0227260:CDS 60.0%
TAGGTCCATGGCGTCAGCCA+AGG + chr2_1:78056273-78056292 Msa0227260:intron 60.0%
CATGGCGTCAGCCAAGGACT+CGG + chr2_1:78056279-78056298 Msa0227260:CDS 60.0%
GGGTACCAAGGTCGACGCAT+AGG - chr2_1:78056645-78056664 None:intergenic 60.0%
GCTCCTGCACCAGACCTTCC+AGG - chr2_1:78056144-78056163 None:intergenic 65.0%
CGAGTCCTTGGCTGACGCCA+TGG - chr2_1:78056281-78056300 None:intergenic 65.0%
GCTCCTGCACCAGACCTTCC+AGG - chr2_1:78056144-78056163 None:intergenic 65.0%
CGAGTCCTTGGCTGACGCCA+TGG - chr2_1:78056281-78056300 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 78056067 78057979 78056067 ID=Msa0227260;Name=Msa0227260
chr2_1 mRNA 78056067 78057979 78056067 ID=Msa0227260-mRNA-1;Parent=Msa0227260;Name=Msa0227260-mRNA-1;_AED=0.00;_eAED=0.00;_QI=14|1|1|1|1|1|5|350|146
chr2_1 exon 78056067 78056191 78056067 ID=Msa0227260-mRNA-1:exon:23760;Parent=Msa0227260-mRNA-1
chr2_1 exon 78056276 78056419 78056276 ID=Msa0227260-mRNA-1:exon:23761;Parent=Msa0227260-mRNA-1
chr2_1 exon 78056851 78056958 78056851 ID=Msa0227260-mRNA-1:exon:23762;Parent=Msa0227260-mRNA-1
chr2_1 exon 78057306 78057357 78057306 ID=Msa0227260-mRNA-1:exon:23763;Parent=Msa0227260-mRNA-1
chr2_1 exon 78057604 78057979 78057604 ID=Msa0227260-mRNA-1:exon:23764;Parent=Msa0227260-mRNA-1
chr2_1 five_prime_UTR 78056067 78056080 78056067 ID=Msa0227260-mRNA-1:five_prime_utr;Parent=Msa0227260-mRNA-1
chr2_1 CDS 78056081 78056191 78056081 ID=Msa0227260-mRNA-1:cds;Parent=Msa0227260-mRNA-1
chr2_1 CDS 78056276 78056419 78056276 ID=Msa0227260-mRNA-1:cds;Parent=Msa0227260-mRNA-1
chr2_1 CDS 78056851 78056958 78056851 ID=Msa0227260-mRNA-1:cds;Parent=Msa0227260-mRNA-1
chr2_1 CDS 78057306 78057357 78057306 ID=Msa0227260-mRNA-1:cds;Parent=Msa0227260-mRNA-1
chr2_1 CDS 78057604 78057629 78057604 ID=Msa0227260-mRNA-1:cds;Parent=Msa0227260-mRNA-1
chr2_1 three_prime_UTR 78057630 78057979 78057630 ID=Msa0227260-mRNA-1:three_prime_utr;Parent=Msa0227260-mRNA-1
Gene Sequence

>Msa0227260

ATGAACAAAATCTTGCAGCGTTCTTGTATCCTAGAATTTGATGGAGCATCCAGTGGAAATCCTGGAAGGTCTGGTGCAGGAGCTGTTCTGCGTTCTGCTGATGGGAGTCGGGTCCATGGCGTCAGCCAAGGACTCGGCACTCAAACAAATAATTCTGCTGAATATAATGGTCTAATTTTGGGGCTGAATGAAGCTAGGAACCAAGGATATGAGCATGTTCATGTCCGAGGTGACTCTCAGCTTGTTTGCAAGCAGTTTGAGGGTTCCTGGAAAGTCAACAACCCAAATTTAAGGAACCTTTGTAATGAGGCTGTGGAGCTGAAGAGTAACTTCAAGTCAGTCAGTGTGGAACATGTTCCTAGGGGTTCTAACTCTGCAGCTGATGCTCAAGCAAATCGCGGCAAAAATCTTGGAGCCGGCGAAGTTGAATATTACTACTGA
Protein sequence

>Msa0227260

MNKILQRSCILEFDGASSGNPGRSGAGAVLRSADGSRVHGVSQGLGTQTNNSAEYNGLILGLNEARNQGYEHVHVRGDSQLVCKQFEGSWKVNNPNLRNLCNEAVELKSNFKSVSVEHVPRGSNSAADAQANRGKNLGAGEVEYYY*