AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0262580

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0262580 MtrunA17_Chr2g0325301 99.291 141 1 0 6 146 8 148 5.25e-101 286
Msa0262580 MtrunA17_Chr6g0451421 58.696 138 57 0 6 143 6 143 1.24e-53 166
Msa0262580 MtrunA17_Chr6g0451411 59.559 136 55 0 8 143 176 311 4.85e-52 167
Msa0262580 MtrunA17_Chr2g0329701 51.429 140 68 0 7 146 233 372 6.36e-46 153
Msa0262580 MtrunA17_Chr7g0264421 51.825 137 66 0 7 143 203 339 1.17e-45 151
Msa0262580 MtrunA17_Chr6g0451441 46.575 146 70 1 6 143 318 463 1.54e-35 127
Msa0262580 MtrunA17_Chr6g0451451 50.000 82 41 0 62 143 1 82 5.83e-25 91.3
Msa0262580 MtrunA17_Chr1g0158081 37.190 121 74 1 11 131 1209 1327 3.24e-16 74.3
Msa0262580 MtrunA17_Chr3g0110591 36.364 121 75 1 11 131 25 143 3.66e-15 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0262580 AT3G01410.2 50.000 136 68 0 8 143 154 289 3.37e-45 149
Msa0262580 AT3G01410.1 50.000 136 68 0 8 143 154 289 3.37e-45 149
Msa0262580 AT5G51080.3 53.285 137 64 0 7 143 121 257 4.79e-45 148
Msa0262580 AT5G51080.1 53.676 136 63 0 8 143 185 320 2.57e-44 148
Msa0262580 AT5G51080.2 53.676 136 63 0 8 143 185 320 2.57e-44 148
Msa0262580 AT1G24090.1 52.143 140 67 0 4 143 212 351 1.92e-41 141

Find 52 sgRNAs with CRISPR-Local

Find 225 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACAAAGGTTCCTTAAATT+TGG 0.088756 2_2:+61591303 None:intergenic
TGAATATAATGGTCTAATTT+TGG 0.114690 2_2:-61591856 Msa0262580:CDS
TGCCTACTAGTTCAGCATTT+AGG 0.223331 2_2:-61590490 Msa0262580:three_prime_UTR
ATTGCTTAAGTGACTTAAAA+TGG 0.233393 2_2:-61590379 Msa0262580:three_prime_UTR
GCCTACTAGTTCAGCATTTA+GGG 0.241265 2_2:-61590489 Msa0262580:three_prime_UTR
TTCTATCTTCTGTCTTCATT+TGG 0.279448 2_2:-61590302 Msa0262580:three_prime_UTR
TACAAAGGTTCCTTAAATTT+GGG 0.313196 2_2:+61591304 None:intergenic
TGGATGCTCCATCAAATTCT+AGG 0.329321 2_2:+61592069 None:intergenic
AAGTCAACAACCCAAATTTA+AGG 0.331211 2_2:-61591314 Msa0262580:CDS
ATTTGGGTTGTTGACTTTCC+AGG 0.336100 2_2:+61591320 None:intergenic
TTTGCAAGTTTGAGGGTTCC+TGG 0.345454 2_2:-61591338 Msa0262580:intron
AATGGTGTTGCTTTGATGTT+TGG 0.346803 2_2:-61590361 Msa0262580:three_prime_UTR
TCTTGTATCCTAGAATTTGA+TGG 0.357172 2_2:-61592077 Msa0262580:CDS
CTAGTAGGCACAACCTAAAC+AGG 0.385949 2_2:+61590503 None:intergenic
AATAATTCTGCTGAATATAA+TGG 0.390357 2_2:-61591867 Msa0262580:CDS
GCTCCTGCACCAGACCTTCC+AGG 0.402463 2_2:+61592038 None:intergenic
TGCGTTCTGCTGATGGGAGT+CGG 0.407658 2_2:-61592010 Msa0262580:CDS
CTCTCAGCTTGTTTGCAAGC+AGG 0.424209 2_2:-61591781 Msa0262580:intron
TTTAAGGAACCTTTGTAATG+AGG 0.445867 2_2:-61591298 Msa0262580:CDS
GCTGTTCTGCGTTCTGCTGA+TGG 0.448826 2_2:-61592017 Msa0262580:CDS
GGAGCATCCAGTGGAAATCC+TGG 0.459158 2_2:-61592056 Msa0262580:CDS
GCGTTCTGCTGATGGGAGTC+GGG 0.465577 2_2:-61592009 Msa0262580:intron
AGTGGAAATCCTGGAAGGTC+TGG 0.475520 2_2:-61592047 Msa0262580:CDS
CTGTTCTGCGTTCTGCTGAT+GGG 0.478692 2_2:-61592016 Msa0262580:CDS
TATGTCTCTTTATAGGTCCA+TGG 0.481724 2_2:-61591918 Msa0262580:intron
TTGTTTGAGTGCCGAGTCCT+TGG 0.487817 2_2:+61591889 None:intergenic
TCAGTGTCGAACATGTTCCT+AGG 0.502100 2_2:-61591245 Msa0262580:CDS
CATGAACATGCTCATATCCT+TGG 0.505728 2_2:+61591814 None:intergenic
CATGGCGTCAGCCAAGGACT+CGG 0.511067 2_2:-61591900 Msa0262580:CDS
TATGACATGGCCAACATGAC+TGG 0.542678 2_2:-61590252 Msa0262580:three_prime_UTR
CCCCTAAATGCTGAACTAGT+AGG 0.549991 2_2:+61590488 None:intergenic
CGAGTCCTTGGCTGACGCCA+TGG 0.551131 2_2:+61591901 None:intergenic
AGACCTTCCAGGATTTCCAC+TGG 0.556723 2_2:+61592049 None:intergenic
TAGGTCCATGGCGTCAGCCA+AGG 0.557191 2_2:-61591906 Msa0262580:CDS
GAACCTTTGTAATGAGGCTG+TGG 0.560005 2_2:-61591292 Msa0262580:CDS
AATCCTGGAAGGTCTGGTGC+AGG 0.565510 2_2:-61592041 Msa0262580:CDS
GTAGTAATATTCAACTTCGC+CGG 0.574495 2_2:+61590585 None:intergenic
ATGGTGAGATATACACATCA+TGG 0.586235 2_2:+61590400 None:intergenic
CTACTAGTTCAGCATTTAGG+GGG 0.587673 2_2:-61590487 Msa0262580:three_prime_UTR
CAGTGTCGAACATGTTCCTA+GGG 0.587867 2_2:-61591244 Msa0262580:intron
CCTACTAGTTCAGCATTTAG+GGG 0.616242 2_2:-61590488 Msa0262580:three_prime_UTR
GCTCCACAGCCTCATTACAA+AGG 0.619284 2_2:+61591289 None:intergenic
TTAACACCATCCAGTCATGT+TGG 0.629124 2_2:+61590242 None:intergenic
CTGAATGAAGCTAGGAACCA+AGG 0.629738 2_2:-61591831 Msa0262580:CDS
GAATTTGATGGAGCATCCAG+TGG 0.630666 2_2:-61592065 Msa0262580:CDS
TATGAGCATGTTCATGTCCG+AGG 0.645245 2_2:-61591807 Msa0262580:CDS
GCTGATGCTCAAGCAAATCG+CGG 0.646058 2_2:-61590869 Msa0262580:CDS
ACATGGCCAACATGACTGGA+TGG 0.655888 2_2:-61590248 Msa0262580:three_prime_UTR
CATCCAGTGGAAATCCTGGA+AGG 0.657104 2_2:-61592052 Msa0262580:CDS
TAATATATTGTGCTATGACA+TGG 0.692388 2_2:-61590265 Msa0262580:three_prime_UTR
AAACAAGCTGAGAGTCACCT+CGG 0.700596 2_2:+61591790 None:intergenic
GAAATTCAGAAACATTCACA+TGG 0.721736 2_2:+61590322 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAACAAAACAAAATAT+AGG + chr2_2:61591723-61591742 None:intergenic 10.0%
!! AAAAAAACAAAACAAAATAT+AGG + chr2_2:61591723-61591742 None:intergenic 10.0%
!!! ATTTACCATCATATTTTTAT+TGG - chr2_2:61590982-61591001 Msa0262580:intron 15.0%
!!! TTTTTATATTTGTATTTGGA+AGG - chr2_2:61591676-61591695 Msa0262580:intron 15.0%
!!! TTTTATATTTGTATTTGGAA+GGG - chr2_2:61591677-61591696 Msa0262580:intron 15.0%
!!! ATTTACCATCATATTTTTAT+TGG - chr2_2:61590982-61591001 Msa0262580:intron 15.0%
!!! TTTTTATATTTGTATTTGGA+AGG - chr2_2:61591676-61591695 Msa0262580:intron 15.0%
!!! TTTTATATTTGTATTTGGAA+GGG - chr2_2:61591677-61591696 Msa0262580:intron 15.0%
!! AATAATTCTGCTGAATATAA+TGG - chr2_2:61590477-61590496 Msa0262580:three_prime_UTR 20.0%
!!! TGAATATAATGGTCTAATTT+TGG - chr2_2:61590488-61590507 Msa0262580:three_prime_UTR 20.0%
!!! GAATATAATGGTCTAATTTT+GGG - chr2_2:61590489-61590508 Msa0262580:three_prime_UTR 20.0%
!!! AATATAATGGTCTAATTTTG+GGG - chr2_2:61590490-61590509 Msa0262580:three_prime_UTR 20.0%
!! TGTTCACAAAATATTATTAC+AGG - chr2_2:61590752-61590771 Msa0262580:intron 20.0%
!! ATGTTGTAATTATTACTACA+TGG - chr2_2:61590853-61590872 Msa0262580:intron 20.0%
!! TATAGTGTTCCTATAATAAT+TGG - chr2_2:61590937-61590956 Msa0262580:intron 20.0%
!! CAAATCAAACCAATTATTAT+AGG + chr2_2:61590949-61590968 None:intergenic 20.0%
!! CAAAACCAATAAAAATATGA+TGG + chr2_2:61590990-61591009 None:intergenic 20.0%
!! TTTATATTTGTATTTGGAAG+GGG - chr2_2:61591678-61591697 Msa0262580:intron 20.0%
!!! TTTTTTTTTTTTTTTGCAGC+CGG - chr2_2:61591740-61591759 Msa0262580:intron 20.0%
!! AATAATTCTGCTGAATATAA+TGG - chr2_2:61590477-61590496 Msa0262580:three_prime_UTR 20.0%
!!! TGAATATAATGGTCTAATTT+TGG - chr2_2:61590488-61590507 Msa0262580:three_prime_UTR 20.0%
!!! GAATATAATGGTCTAATTTT+GGG - chr2_2:61590489-61590508 Msa0262580:three_prime_UTR 20.0%
!!! AATATAATGGTCTAATTTTG+GGG - chr2_2:61590490-61590509 Msa0262580:three_prime_UTR 20.0%
!! TGTTCACAAAATATTATTAC+AGG - chr2_2:61590752-61590771 Msa0262580:intron 20.0%
!! ATGTTGTAATTATTACTACA+TGG - chr2_2:61590853-61590872 Msa0262580:intron 20.0%
!! TATAGTGTTCCTATAATAAT+TGG - chr2_2:61590937-61590956 Msa0262580:intron 20.0%
!! CAAATCAAACCAATTATTAT+AGG + chr2_2:61590949-61590968 None:intergenic 20.0%
!! CAAAACCAATAAAAATATGA+TGG + chr2_2:61590990-61591009 None:intergenic 20.0%
!! TTTATATTTGTATTTGGAAG+GGG - chr2_2:61591678-61591697 Msa0262580:intron 20.0%
!!! TTTTTTTTTTTTTTTGCAGC+CGG - chr2_2:61591740-61591759 Msa0262580:intron 20.0%
! ATAGTGCAAAAATGATTTGA+TGG + chr2_2:61590373-61590392 None:intergenic 25.0%
! AACACTTTATGTCTCTTTAT+AGG - chr2_2:61590419-61590438 Msa0262580:three_prime_UTR 25.0%
! ATGAGTTATAATAGACGTAA+GGG + chr2_2:61590595-61590614 None:intergenic 25.0%
!! AAATCACCGTGTAATATTTT+GGG + chr2_2:61590830-61590849 None:intergenic 25.0%
! ACACTATATATCAAGCAATT+AGG + chr2_2:61590925-61590944 None:intergenic 25.0%
! TACAAAGGTTCCTTAAATTT+GGG + chr2_2:61591043-61591062 None:intergenic 25.0%
! TTACAAAGGTTCCTTAAATT+TGG + chr2_2:61591044-61591063 None:intergenic 25.0%
!!! TCATGTTTTTGTATTGTTGA+AGG - chr2_2:61591438-61591457 Msa0262580:intron 25.0%
!!! CATGTTTTTGTATTGTTGAA+GGG - chr2_2:61591439-61591458 Msa0262580:intron 25.0%
! AAACATCTTTCACTTGTATT+TGG - chr2_2:61591610-61591629 Msa0262580:intron 25.0%
!! CTATGTTAATTTTCCTGTTT+AGG - chr2_2:61591828-61591847 Msa0262580:CDS 25.0%
!!! ATTTTAAGTCACTTAAGCAA+TGG + chr2_2:61591966-61591985 None:intergenic 25.0%
! ATTGCTTAAGTGACTTAAAA+TGG - chr2_2:61591965-61591984 Msa0262580:intron 25.0%
! TAATATATTGTGCTATGACA+TGG - chr2_2:61592079-61592098 Msa0262580:CDS 25.0%
! ATAGTGCAAAAATGATTTGA+TGG + chr2_2:61590373-61590392 None:intergenic 25.0%
! AACACTTTATGTCTCTTTAT+AGG - chr2_2:61590419-61590438 Msa0262580:three_prime_UTR 25.0%
! ATGAGTTATAATAGACGTAA+GGG + chr2_2:61590595-61590614 None:intergenic 25.0%
!! AAATCACCGTGTAATATTTT+GGG + chr2_2:61590830-61590849 None:intergenic 25.0%
! ACACTATATATCAAGCAATT+AGG + chr2_2:61590925-61590944 None:intergenic 25.0%
! TACAAAGGTTCCTTAAATTT+GGG + chr2_2:61591043-61591062 None:intergenic 25.0%
! TTACAAAGGTTCCTTAAATT+TGG + chr2_2:61591044-61591063 None:intergenic 25.0%
!!! TCATGTTTTTGTATTGTTGA+AGG - chr2_2:61591438-61591457 Msa0262580:intron 25.0%
!!! CATGTTTTTGTATTGTTGAA+GGG - chr2_2:61591439-61591458 Msa0262580:intron 25.0%
! AAACATCTTTCACTTGTATT+TGG - chr2_2:61591610-61591629 Msa0262580:intron 25.0%
!! CTATGTTAATTTTCCTGTTT+AGG - chr2_2:61591828-61591847 Msa0262580:CDS 25.0%
!!! ATTTTAAGTCACTTAAGCAA+TGG + chr2_2:61591966-61591985 None:intergenic 25.0%
! ATTGCTTAAGTGACTTAAAA+TGG - chr2_2:61591965-61591984 Msa0262580:intron 25.0%
! TAATATATTGTGCTATGACA+TGG - chr2_2:61592079-61592098 Msa0262580:CDS 25.0%
TCTTGTATCCTAGAATTTGA+TGG - chr2_2:61590267-61590286 Msa0262580:three_prime_UTR 30.0%
GATGAGTTATAATAGACGTA+AGG + chr2_2:61590596-61590615 None:intergenic 30.0%
TGTTAATGCATACACTGTAA+GGG + chr2_2:61590651-61590670 None:intergenic 30.0%
ATGTTAATGCATACACTGTA+AGG + chr2_2:61590652-61590671 None:intergenic 30.0%
CATCACTCATTTCATTCATA+TGG + chr2_2:61590709-61590728 None:intergenic 30.0%
TTGGTACCCAAAATATTACA+CGG - chr2_2:61590821-61590840 Msa0262580:intron 30.0%
! CAAATCACCGTGTAATATTT+TGG + chr2_2:61590831-61590850 None:intergenic 30.0%
TATATATCAAGCAATTAGGC+AGG + chr2_2:61590921-61590940 None:intergenic 30.0%
!!! TTATTGGTTTTGCAAGTTTG+AGG - chr2_2:61590998-61591017 Msa0262580:intron 30.0%
!!! TATTGGTTTTGCAAGTTTGA+GGG - chr2_2:61590999-61591018 Msa0262580:intron 30.0%
AAGTCAACAACCCAAATTTA+AGG - chr2_2:61591030-61591049 Msa0262580:intron 30.0%
TTTAAGGAACCTTTGTAATG+AGG - chr2_2:61591046-61591065 Msa0262580:intron 30.0%
! GTTTCTCTGGAAAACTTAAA+AGG - chr2_2:61591207-61591226 Msa0262580:intron 30.0%
CATAAACGATTCTCAAATGT+TGG + chr2_2:61591257-61591276 None:intergenic 30.0%
! GTTTTTCCTGTTAAAGCATA+TGG + chr2_2:61591338-61591357 None:intergenic 30.0%
!!! CAAGACTTTTGAGTTCTTAT+TGG - chr2_2:61591523-61591542 Msa0262580:intron 30.0%
!!! ATGTTTTTCTAAAGCAGCTT+TGG + chr2_2:61591596-61591615 None:intergenic 30.0%
! TGATGTTTGGATTTTATGCT+TGG - chr2_2:61591996-61592015 Msa0262580:intron 30.0%
GAAATTCAGAAACATTCACA+TGG + chr2_2:61592025-61592044 None:intergenic 30.0%
TTCTATCTTCTGTCTTCATT+TGG - chr2_2:61592042-61592061 Msa0262580:CDS 30.0%
! TGTTCATGTCATTTCATAGA+TGG + chr2_2:61590233-61590252 None:intergenic 30.0%
TCTTGTATCCTAGAATTTGA+TGG - chr2_2:61590267-61590286 Msa0262580:three_prime_UTR 30.0%
GATGAGTTATAATAGACGTA+AGG + chr2_2:61590596-61590615 None:intergenic 30.0%
TGTTAATGCATACACTGTAA+GGG + chr2_2:61590651-61590670 None:intergenic 30.0%
ATGTTAATGCATACACTGTA+AGG + chr2_2:61590652-61590671 None:intergenic 30.0%
CATCACTCATTTCATTCATA+TGG + chr2_2:61590709-61590728 None:intergenic 30.0%
TTGGTACCCAAAATATTACA+CGG - chr2_2:61590821-61590840 Msa0262580:intron 30.0%
! CAAATCACCGTGTAATATTT+TGG + chr2_2:61590831-61590850 None:intergenic 30.0%
TATATATCAAGCAATTAGGC+AGG + chr2_2:61590921-61590940 None:intergenic 30.0%
!!! TTATTGGTTTTGCAAGTTTG+AGG - chr2_2:61590998-61591017 Msa0262580:intron 30.0%
!!! TATTGGTTTTGCAAGTTTGA+GGG - chr2_2:61590999-61591018 Msa0262580:intron 30.0%
AAGTCAACAACCCAAATTTA+AGG - chr2_2:61591030-61591049 Msa0262580:intron 30.0%
TTTAAGGAACCTTTGTAATG+AGG - chr2_2:61591046-61591065 Msa0262580:intron 30.0%
! GTTTCTCTGGAAAACTTAAA+AGG - chr2_2:61591207-61591226 Msa0262580:intron 30.0%
CATAAACGATTCTCAAATGT+TGG + chr2_2:61591257-61591276 None:intergenic 30.0%
! GTTTTTCCTGTTAAAGCATA+TGG + chr2_2:61591338-61591357 None:intergenic 30.0%
!!! CAAGACTTTTGAGTTCTTAT+TGG - chr2_2:61591523-61591542 Msa0262580:intron 30.0%
!!! ATGTTTTTCTAAAGCAGCTT+TGG + chr2_2:61591596-61591615 None:intergenic 30.0%
! TGATGTTTGGATTTTATGCT+TGG - chr2_2:61591996-61592015 Msa0262580:intron 30.0%
GAAATTCAGAAACATTCACA+TGG + chr2_2:61592025-61592044 None:intergenic 30.0%
TTCTATCTTCTGTCTTCATT+TGG - chr2_2:61592042-61592061 Msa0262580:CDS 30.0%
TATGTCTCTTTATAGGTCCA+TGG - chr2_2:61590426-61590445 Msa0262580:three_prime_UTR 35.0%
! TAAATTCCTTGTTTGCAAGC+AGG - chr2_2:61590619-61590638 Msa0262580:intron 35.0%
!! GTGTAATATTTTGGGTACCA+AGG + chr2_2:61590822-61590841 None:intergenic 35.0%
GGTATACACTTTGTCTCATT+TGG - chr2_2:61591121-61591140 Msa0262580:intron 35.0%
AGAGAAACACACAATCAAGA+AGG + chr2_2:61591195-61591214 None:intergenic 35.0%
TTCTTGATTGTGTGTTTCTC+TGG - chr2_2:61591194-61591213 Msa0262580:intron 35.0%
CTGGAAAACTTAAAAGGTTC+AGG - chr2_2:61591213-61591232 Msa0262580:intron 35.0%
! GAGAGGCATGCTAATTAAAA+AGG + chr2_2:61591298-61591317 None:intergenic 35.0%
TCACTTGTATTTGGTTGCAA+TGG - chr2_2:61591619-61591638 Msa0262580:intron 35.0%
GTAGTAATATTCAACTTCGC+CGG + chr2_2:61591762-61591781 None:intergenic 35.0%
ATGCCACTGTCAAAAACTAT+TGG - chr2_2:61591802-61591821 Msa0262580:CDS 35.0%
! TTGCCAATAGTTTTTGACAG+TGG + chr2_2:61591808-61591827 None:intergenic 35.0%
ATGGTGAGATATACACATCA+TGG + chr2_2:61591947-61591966 None:intergenic 35.0%
!! AATGGTGTTGCTTTGATGTT+TGG - chr2_2:61591983-61592002 Msa0262580:intron 35.0%
TATGTCTCTTTATAGGTCCA+TGG - chr2_2:61590426-61590445 Msa0262580:three_prime_UTR 35.0%
! TAAATTCCTTGTTTGCAAGC+AGG - chr2_2:61590619-61590638 Msa0262580:intron 35.0%
!! GTGTAATATTTTGGGTACCA+AGG + chr2_2:61590822-61590841 None:intergenic 35.0%
GGTATACACTTTGTCTCATT+TGG - chr2_2:61591121-61591140 Msa0262580:intron 35.0%
AGAGAAACACACAATCAAGA+AGG + chr2_2:61591195-61591214 None:intergenic 35.0%
TTCTTGATTGTGTGTTTCTC+TGG - chr2_2:61591194-61591213 Msa0262580:intron 35.0%
CTGGAAAACTTAAAAGGTTC+AGG - chr2_2:61591213-61591232 Msa0262580:intron 35.0%
! GAGAGGCATGCTAATTAAAA+AGG + chr2_2:61591298-61591317 None:intergenic 35.0%
TCACTTGTATTTGGTTGCAA+TGG - chr2_2:61591619-61591638 Msa0262580:intron 35.0%
GTAGTAATATTCAACTTCGC+CGG + chr2_2:61591762-61591781 None:intergenic 35.0%
ATGCCACTGTCAAAAACTAT+TGG - chr2_2:61591802-61591821 Msa0262580:CDS 35.0%
! TTGCCAATAGTTTTTGACAG+TGG + chr2_2:61591808-61591827 None:intergenic 35.0%
ATGGTGAGATATACACATCA+TGG + chr2_2:61591947-61591966 None:intergenic 35.0%
!! AATGGTGTTGCTTTGATGTT+TGG - chr2_2:61591983-61592002 Msa0262580:intron 35.0%
TGGATGCTCCATCAAATTCT+AGG + chr2_2:61590278-61590297 None:intergenic 40.0%
CATGAACATGCTCATATCCT+TGG + chr2_2:61590533-61590552 None:intergenic 40.0%
AACTAACCTGCTTGCAAACA+AGG + chr2_2:61590572-61590591 None:intergenic 40.0%
TCTGCATTAACAGCAATGCA+AGG - chr2_2:61590878-61590897 Msa0262580:CDS 40.0%
!! ATTTGGGTTGTTGACTTTCC+AGG + chr2_2:61591027-61591046 None:intergenic 40.0%
TGAGACAAAGTGTATACCCT+AGG + chr2_2:61591119-61591138 None:intergenic 40.0%
! TAGTTTTTGACAGTGGCATG+CGG + chr2_2:61591801-61591820 None:intergenic 40.0%
TGCCTACTAGTTCAGCATTT+AGG - chr2_2:61591854-61591873 Msa0262580:CDS 40.0%
GCCTACTAGTTCAGCATTTA+GGG - chr2_2:61591855-61591874 Msa0262580:CDS 40.0%
CCTACTAGTTCAGCATTTAG+GGG - chr2_2:61591856-61591875 Msa0262580:CDS 40.0%
CTACTAGTTCAGCATTTAGG+GGG - chr2_2:61591857-61591876 Msa0262580:CDS 40.0%
TTAACACCATCCAGTCATGT+TGG + chr2_2:61592105-61592124 None:intergenic 40.0%
TGGATGCTCCATCAAATTCT+AGG + chr2_2:61590278-61590297 None:intergenic 40.0%
CATGAACATGCTCATATCCT+TGG + chr2_2:61590533-61590552 None:intergenic 40.0%
AACTAACCTGCTTGCAAACA+AGG + chr2_2:61590572-61590591 None:intergenic 40.0%
TCTGCATTAACAGCAATGCA+AGG - chr2_2:61590878-61590897 Msa0262580:CDS 40.0%
!! ATTTGGGTTGTTGACTTTCC+AGG + chr2_2:61591027-61591046 None:intergenic 40.0%
TGAGACAAAGTGTATACCCT+AGG + chr2_2:61591119-61591138 None:intergenic 40.0%
! TAGTTTTTGACAGTGGCATG+CGG + chr2_2:61591801-61591820 None:intergenic 40.0%
TGCCTACTAGTTCAGCATTT+AGG - chr2_2:61591854-61591873 Msa0262580:CDS 40.0%
GCCTACTAGTTCAGCATTTA+GGG - chr2_2:61591855-61591874 Msa0262580:CDS 40.0%
CCTACTAGTTCAGCATTTAG+GGG - chr2_2:61591856-61591875 Msa0262580:CDS 40.0%
CTACTAGTTCAGCATTTAGG+GGG - chr2_2:61591857-61591876 Msa0262580:CDS 40.0%
TTAACACCATCCAGTCATGT+TGG + chr2_2:61592105-61592124 None:intergenic 40.0%
! GAATTTGATGGAGCATCCAG+TGG - chr2_2:61590279-61590298 Msa0262580:three_prime_UTR 45.0%
!!! TTTTGGGGCTGAATGAAGCT+AGG - chr2_2:61590505-61590524 Msa0262580:three_prime_UTR 45.0%
CTGAATGAAGCTAGGAACCA+AGG - chr2_2:61590513-61590532 Msa0262580:three_prime_UTR 45.0%
TATGAGCATGTTCATGTCCG+AGG - chr2_2:61590537-61590556 Msa0262580:three_prime_UTR 45.0%
AAACAAGCTGAGAGTCACCT+CGG + chr2_2:61590557-61590576 None:intergenic 45.0%
TTTGCAAGTTTGAGGGTTCC+TGG - chr2_2:61591006-61591025 Msa0262580:intron 45.0%
GAACCTTTGTAATGAGGCTG+TGG - chr2_2:61591052-61591071 Msa0262580:intron 45.0%
! TCAGTGTCGAACATGTTCCT+AGG - chr2_2:61591099-61591118 Msa0262580:intron 45.0%
! CAGTGTCGAACATGTTCCTA+GGG - chr2_2:61591100-61591119 Msa0262580:intron 45.0%
ATCGCGTGACTTATGTAGAG+AGG + chr2_2:61591315-61591334 None:intergenic 45.0%
CGCGATCCATATGCTTTAAC+AGG - chr2_2:61591329-61591348 Msa0262580:CDS 45.0%
GCAAATCGCGGCAAAAATCT+TGG - chr2_2:61591487-61591506 Msa0262580:intron 45.0%
AATCGCGGCAAAAATCTTGG+AGG - chr2_2:61591490-61591509 Msa0262580:intron 45.0%
CTAGTAGGCACAACCTAAAC+AGG + chr2_2:61591844-61591863 None:intergenic 45.0%
CCCCTAAATGCTGAACTAGT+AGG + chr2_2:61591859-61591878 None:intergenic 45.0%
TATGACATGGCCAACATGAC+TGG - chr2_2:61592092-61592111 Msa0262580:CDS 45.0%
! GAATTTGATGGAGCATCCAG+TGG - chr2_2:61590279-61590298 Msa0262580:three_prime_UTR 45.0%
!!! TTTTGGGGCTGAATGAAGCT+AGG - chr2_2:61590505-61590524 Msa0262580:three_prime_UTR 45.0%
CTGAATGAAGCTAGGAACCA+AGG - chr2_2:61590513-61590532 Msa0262580:three_prime_UTR 45.0%
TATGAGCATGTTCATGTCCG+AGG - chr2_2:61590537-61590556 Msa0262580:three_prime_UTR 45.0%
AAACAAGCTGAGAGTCACCT+CGG + chr2_2:61590557-61590576 None:intergenic 45.0%
TTTGCAAGTTTGAGGGTTCC+TGG - chr2_2:61591006-61591025 Msa0262580:intron 45.0%
GAACCTTTGTAATGAGGCTG+TGG - chr2_2:61591052-61591071 Msa0262580:intron 45.0%
! TCAGTGTCGAACATGTTCCT+AGG - chr2_2:61591099-61591118 Msa0262580:intron 45.0%
! CAGTGTCGAACATGTTCCTA+GGG - chr2_2:61591100-61591119 Msa0262580:intron 45.0%
ATCGCGTGACTTATGTAGAG+AGG + chr2_2:61591315-61591334 None:intergenic 45.0%
CGCGATCCATATGCTTTAAC+AGG - chr2_2:61591329-61591348 Msa0262580:CDS 45.0%
GCAAATCGCGGCAAAAATCT+TGG - chr2_2:61591487-61591506 Msa0262580:intron 45.0%
AATCGCGGCAAAAATCTTGG+AGG - chr2_2:61591490-61591509 Msa0262580:intron 45.0%
CTAGTAGGCACAACCTAAAC+AGG + chr2_2:61591844-61591863 None:intergenic 45.0%
CCCCTAAATGCTGAACTAGT+AGG + chr2_2:61591859-61591878 None:intergenic 45.0%
TATGACATGGCCAACATGAC+TGG - chr2_2:61592092-61592111 Msa0262580:CDS 45.0%
!!! ATTTTTTTTATATTTGTATT+TGG - chr2_2:61591672-61591691 Msa0262580:intron 5.0%
!!! ATTTTTTTTATATTTGTATT+TGG - chr2_2:61591672-61591691 Msa0262580:intron 5.0%
CATCCAGTGGAAATCCTGGA+AGG - chr2_2:61590292-61590311 Msa0262580:three_prime_UTR 50.0%
AGACCTTCCAGGATTTCCAC+TGG + chr2_2:61590298-61590317 None:intergenic 50.0%
AGTGGAAATCCTGGAAGGTC+TGG - chr2_2:61590297-61590316 Msa0262580:three_prime_UTR 50.0%
CTGTTCTGCGTTCTGCTGAT+GGG - chr2_2:61590328-61590347 Msa0262580:three_prime_UTR 50.0%
!! TTGTTTGAGTGCCGAGTCCT+TGG + chr2_2:61590458-61590477 None:intergenic 50.0%
! CTCTCAGCTTGTTTGCAAGC+AGG - chr2_2:61590563-61590582 Msa0262580:exon 50.0%
!! GTTTTCCTATGCGTCGACCT+TGG - chr2_2:61590802-61590821 Msa0262580:intron 50.0%
GCTCCACAGCCTCATTACAA+AGG + chr2_2:61591058-61591077 None:intergenic 50.0%
GCTGATGCTCAAGCAAATCG+CGG - chr2_2:61591475-61591494 Msa0262580:intron 50.0%
ACATGGCCAACATGACTGGA+TGG - chr2_2:61592096-61592115 Msa0262580:CDS 50.0%
CATCCAGTGGAAATCCTGGA+AGG - chr2_2:61590292-61590311 Msa0262580:three_prime_UTR 50.0%
AGACCTTCCAGGATTTCCAC+TGG + chr2_2:61590298-61590317 None:intergenic 50.0%
AGTGGAAATCCTGGAAGGTC+TGG - chr2_2:61590297-61590316 Msa0262580:three_prime_UTR 50.0%
CTGTTCTGCGTTCTGCTGAT+GGG - chr2_2:61590328-61590347 Msa0262580:three_prime_UTR 50.0%
!! TTGTTTGAGTGCCGAGTCCT+TGG + chr2_2:61590458-61590477 None:intergenic 50.0%
! CTCTCAGCTTGTTTGCAAGC+AGG - chr2_2:61590563-61590582 Msa0262580:exon 50.0%
!! GTTTTCCTATGCGTCGACCT+TGG - chr2_2:61590802-61590821 Msa0262580:intron 50.0%
GCTCCACAGCCTCATTACAA+AGG + chr2_2:61591058-61591077 None:intergenic 50.0%
GCTGATGCTCAAGCAAATCG+CGG - chr2_2:61591475-61591494 Msa0262580:intron 50.0%
ACATGGCCAACATGACTGGA+TGG - chr2_2:61592096-61592115 Msa0262580:CDS 50.0%
! GGAGCATCCAGTGGAAATCC+TGG - chr2_2:61590288-61590307 Msa0262580:three_prime_UTR 55.0%
AATCCTGGAAGGTCTGGTGC+AGG - chr2_2:61590303-61590322 Msa0262580:three_prime_UTR 55.0%
GCTGTTCTGCGTTCTGCTGA+TGG - chr2_2:61590327-61590346 Msa0262580:three_prime_UTR 55.0%
TGCGTTCTGCTGATGGGAGT+CGG - chr2_2:61590334-61590353 Msa0262580:three_prime_UTR 55.0%
! GGAGCATCCAGTGGAAATCC+TGG - chr2_2:61590288-61590307 Msa0262580:three_prime_UTR 55.0%
AATCCTGGAAGGTCTGGTGC+AGG - chr2_2:61590303-61590322 Msa0262580:three_prime_UTR 55.0%
GCTGTTCTGCGTTCTGCTGA+TGG - chr2_2:61590327-61590346 Msa0262580:three_prime_UTR 55.0%
TGCGTTCTGCTGATGGGAGT+CGG - chr2_2:61590334-61590353 Msa0262580:three_prime_UTR 55.0%
GCGTTCTGCTGATGGGAGTC+GGG - chr2_2:61590335-61590354 Msa0262580:three_prime_UTR 60.0%
TAGGTCCATGGCGTCAGCCA+AGG - chr2_2:61590438-61590457 Msa0262580:three_prime_UTR 60.0%
CATGGCGTCAGCCAAGGACT+CGG - chr2_2:61590444-61590463 Msa0262580:three_prime_UTR 60.0%
GGGTACCAAGGTCGACGCAT+AGG + chr2_2:61590810-61590829 None:intergenic 60.0%
GCGTTCTGCTGATGGGAGTC+GGG - chr2_2:61590335-61590354 Msa0262580:three_prime_UTR 60.0%
TAGGTCCATGGCGTCAGCCA+AGG - chr2_2:61590438-61590457 Msa0262580:three_prime_UTR 60.0%
CATGGCGTCAGCCAAGGACT+CGG - chr2_2:61590444-61590463 Msa0262580:three_prime_UTR 60.0%
GGGTACCAAGGTCGACGCAT+AGG + chr2_2:61590810-61590829 None:intergenic 60.0%
GCTCCTGCACCAGACCTTCC+AGG + chr2_2:61590309-61590328 None:intergenic 65.0%
CGAGTCCTTGGCTGACGCCA+TGG + chr2_2:61590446-61590465 None:intergenic 65.0%
GCTCCTGCACCAGACCTTCC+AGG + chr2_2:61590309-61590328 None:intergenic 65.0%
CGAGTCCTTGGCTGACGCCA+TGG + chr2_2:61590446-61590465 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 61590232 61592134 61590232 ID=Msa0262580;Name=Msa0262580
chr2_2 mRNA 61590232 61592134 61590232 ID=Msa0262580-mRNA-1;Parent=Msa0262580;Name=Msa0262580-mRNA-1;_AED=0.00;_eAED=0.00;_QI=14|1|1|1|1|1|5|350|146
chr2_2 exon 61590232 61590607 61590232 ID=Msa0262580-mRNA-1:exon:18979;Parent=Msa0262580-mRNA-1
chr2_2 exon 61590855 61590906 61590855 ID=Msa0262580-mRNA-1:exon:18978;Parent=Msa0262580-mRNA-1
chr2_2 exon 61591245 61591352 61591245 ID=Msa0262580-mRNA-1:exon:18977;Parent=Msa0262580-mRNA-1
chr2_2 exon 61591782 61591925 61591782 ID=Msa0262580-mRNA-1:exon:18976;Parent=Msa0262580-mRNA-1
chr2_2 exon 61592010 61592134 61592010 ID=Msa0262580-mRNA-1:exon:18975;Parent=Msa0262580-mRNA-1
chr2_2 five_prime_UTR 61592121 61592134 61592121 ID=Msa0262580-mRNA-1:five_prime_utr;Parent=Msa0262580-mRNA-1
chr2_2 CDS 61592010 61592120 61592010 ID=Msa0262580-mRNA-1:cds;Parent=Msa0262580-mRNA-1
chr2_2 CDS 61591782 61591925 61591782 ID=Msa0262580-mRNA-1:cds;Parent=Msa0262580-mRNA-1
chr2_2 CDS 61591245 61591352 61591245 ID=Msa0262580-mRNA-1:cds;Parent=Msa0262580-mRNA-1
chr2_2 CDS 61590855 61590906 61590855 ID=Msa0262580-mRNA-1:cds;Parent=Msa0262580-mRNA-1
chr2_2 CDS 61590582 61590607 61590582 ID=Msa0262580-mRNA-1:cds;Parent=Msa0262580-mRNA-1
chr2_2 three_prime_UTR 61590232 61590581 61590232 ID=Msa0262580-mRNA-1:three_prime_utr;Parent=Msa0262580-mRNA-1
Gene Sequence

>Msa0262580

ATGAACAAAATCTTGCAGCGTTCTTGTATCCTAGAATTTGATGGAGCATCCAGTGGAAATCCTGGAAGGTCTGGTGCAGGAGCTGTTCTGCGTTCTGCTGATGGGAGTCGGGTCCATGGCGTCAGCCAAGGACTCGGCACTCAAACAAATAATTCTGCTGAATATAATGGTCTAATTTTGGGGCTGAATGAAGCTAGGAACCAAGGATATGAGCATGTTCATGTCCGAGGTGACTCTCAGCTTGTTTGCAAGCAGTTTGAGGGTTCCTGGAAAGTCAACAACCCAAATTTAAGGAACCTTTGTAATGAGGCTGTGGAGCTGAAGAGTAACTTCAAGTCAGTCAGTGTCGAACATGTTCCTAGGGGTTCTAACTCTGCAGCTGATGCTCAAGCAAATCGCGGCAAAAATCTTGGAGCCGGCGAAGTTGAATATTACTACTGA
Protein sequence

>Msa0262580

MNKILQRSCILEFDGASSGNPGRSGAGAVLRSADGSRVHGVSQGLGTQTNNSAEYNGLILGLNEARNQGYEHVHVRGDSQLVCKQFEGSWKVNNPNLRNLCNEAVELKSNFKSVSVEHVPRGSNSAADAQANRGKNLGAGEVEYYY*