AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0306050

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0306050 MtrunA17_Chr2g0325301 99.291 141 1 0 6 146 8 148 5.25e-101 286
Msa0306050 MtrunA17_Chr6g0451421 58.696 138 57 0 6 143 6 143 1.24e-53 166
Msa0306050 MtrunA17_Chr6g0451411 59.559 136 55 0 8 143 176 311 4.85e-52 167
Msa0306050 MtrunA17_Chr2g0329701 51.429 140 68 0 7 146 233 372 6.36e-46 153
Msa0306050 MtrunA17_Chr7g0264421 51.825 137 66 0 7 143 203 339 1.17e-45 151
Msa0306050 MtrunA17_Chr6g0451441 46.575 146 70 1 6 143 318 463 1.54e-35 127
Msa0306050 MtrunA17_Chr6g0451451 50.000 82 41 0 62 143 1 82 5.83e-25 91.3
Msa0306050 MtrunA17_Chr1g0158081 37.190 121 74 1 11 131 1209 1327 3.24e-16 74.3
Msa0306050 MtrunA17_Chr3g0110591 36.364 121 75 1 11 131 25 143 3.66e-15 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0306050 AT3G01410.2 50.000 136 68 0 8 143 154 289 3.37e-45 149
Msa0306050 AT3G01410.1 50.000 136 68 0 8 143 154 289 3.37e-45 149
Msa0306050 AT5G51080.3 53.285 137 64 0 7 143 121 257 4.79e-45 148
Msa0306050 AT5G51080.1 53.676 136 63 0 8 143 185 320 2.57e-44 148
Msa0306050 AT5G51080.2 53.676 136 63 0 8 143 185 320 2.57e-44 148
Msa0306050 AT1G24090.1 52.143 140 67 0 4 143 212 351 1.92e-41 141

Find 51 sgRNAs with CRISPR-Local

Find 230 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACAAAGGTTCCTTAAATT+TGG 0.088756 2_3:+64310143 None:intergenic
TGAATATAATGGTCTAATTT+TGG 0.114690 2_3:-64310698 Msa0306050:CDS
GTGCCTACTGTTCAGCATTT+AGG 0.149570 2_3:-64309332 Msa0306050:three_prime_UTR
ATTGCTTAAGTGACTTAAAA+TGG 0.233393 2_3:-64309221 Msa0306050:three_prime_UTR
TGCCTACTGTTCAGCATTTA+GGG 0.244008 2_3:-64309331 Msa0306050:three_prime_UTR
TGTCTTCATCTGTCTTCATT+TGG 0.308569 2_3:-64309135 Msa0306050:three_prime_UTR
TACAAAGGTTCCTTAAATTT+GGG 0.313196 2_3:+64310144 None:intergenic
TGCAACAGTTTGAGGGTTCC+TGG 0.323750 2_3:-64310178 Msa0306050:intron
TGGATGCTCCATCAAATTCT+AGG 0.329321 2_3:+64310911 None:intergenic
AAGTCAACAACCCAAATTTA+AGG 0.331211 2_3:-64310154 Msa0306050:CDS
ATTTGGGTTGTTGACTTTCC+AGG 0.336100 2_3:+64310160 None:intergenic
AATGGTGTTGCTTTGATGTT+TGG 0.346803 2_3:-64309203 Msa0306050:three_prime_UTR
TCTTGTATCCTAGAATTTGA+TGG 0.357172 2_3:-64310919 Msa0306050:CDS
AATAATTCTGCTGAATATAA+TGG 0.390357 2_3:-64310709 Msa0306050:CDS
GCTCCTGCACCAGACCTTCC+AGG 0.402463 2_3:+64310880 None:intergenic
TGCGTTCTGCTGATGGGAGT+CGG 0.407658 2_3:-64310852 Msa0306050:CDS
CTCTCAGCTTGTTTGCAAGC+AGG 0.424209 2_3:-64310623 Msa0306050:intron
ACAGTAGGCACAACCTAAAC+AGG 0.426093 2_3:+64309344 None:intergenic
TTTAAGGAACCTTTGTAATG+AGG 0.445867 2_3:-64310138 Msa0306050:CDS
GCTGTTCTGCGTTCTGCTGA+TGG 0.448826 2_3:-64310859 Msa0306050:CDS
TATGTCTCTTTGTAGGTCCA+TGG 0.454685 2_3:-64310760 Msa0306050:intron
GGAGCATCCAGTGGAAATCC+TGG 0.459158 2_3:-64310898 Msa0306050:CDS
GCGTTCTGCTGATGGGAGTC+GGG 0.465577 2_3:-64310851 Msa0306050:intron
AGTGGAAATCCTGGAAGGTC+TGG 0.475520 2_3:-64310889 Msa0306050:CDS
CTGTTCTGCGTTCTGCTGAT+GGG 0.478692 2_3:-64310858 Msa0306050:CDS
TTGTTTGAGTGCCGAGTCCT+TGG 0.487817 2_3:+64310731 None:intergenic
TCAGTGTCGAACATGTTCCT+AGG 0.502100 2_3:-64310085 Msa0306050:CDS
CATGAACATGCTCATATCCT+TGG 0.505728 2_3:+64310656 None:intergenic
CATGGCGTCAGCCAAGGACT+CGG 0.511067 2_3:-64310742 Msa0306050:CDS
TATGACATGGCCAACATGAC+TGG 0.542678 2_3:-64309085 Msa0306050:three_prime_UTR
CGAGTCCTTGGCTGACGCCA+TGG 0.551131 2_3:+64310743 None:intergenic
TAGGTCCATGGCGTCAGCCA+AGG 0.555171 2_3:-64310748 Msa0306050:CDS
AGACCTTCCAGGATTTCCAC+TGG 0.556723 2_3:+64310891 None:intergenic
GCCTACTGTTCAGCATTTAG+GGG 0.559030 2_3:-64309330 Msa0306050:three_prime_UTR
GAACCTTTGTAATGAGGCTG+TGG 0.560005 2_3:-64310132 Msa0306050:CDS
CCTACTGTTCAGCATTTAGG+GGG 0.564780 2_3:-64309329 Msa0306050:three_prime_UTR
AATCCTGGAAGGTCTGGTGC+AGG 0.565510 2_3:-64310883 Msa0306050:CDS
GTAGTAATATTCAACTTCGC+CGG 0.574495 2_3:+64309426 None:intergenic
ATGGTGAGATATACACATCA+TGG 0.586235 2_3:+64309242 None:intergenic
CAGTGTCGAACATGTTCCTA+GGG 0.587867 2_3:-64310084 Msa0306050:intron
GCTCCACAGCCTCATTACAA+AGG 0.619284 2_3:+64310129 None:intergenic
TTAACACCATCCAGTCATGT+TGG 0.629124 2_3:+64309075 None:intergenic
CTGAATGAAGCTAGGAACCA+AGG 0.629738 2_3:-64310673 Msa0306050:CDS
GAATTTGATGGAGCATCCAG+TGG 0.630666 2_3:-64310907 Msa0306050:CDS
TATGAGCATGTTCATGTCCG+AGG 0.645245 2_3:-64310649 Msa0306050:CDS
GCTGATGCTCAAGCAAATCG+CGG 0.646058 2_3:-64309712 Msa0306050:CDS
ACATGGCCAACATGACTGGA+TGG 0.655888 2_3:-64309081 Msa0306050:three_prime_UTR
CATCCAGTGGAAATCCTGGA+AGG 0.657104 2_3:-64310894 Msa0306050:CDS
TAATATATTGTGCTATGACA+TGG 0.692388 2_3:-64309098 Msa0306050:three_prime_UTR
AAACAAGCTGAGAGTCACCT+CGG 0.700596 2_3:+64310632 None:intergenic
AGAATTCAGAAACATTCACA+TGG 0.730794 2_3:+64309164 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAATATTTTGTGAACAAT+CGG + chr2_3:64309583-64309602 None:intergenic 15.0%
!!! ATTTACCATCATATTTTTAT+TGG - chr2_3:64309815-64309834 Msa0306050:intron 15.0%
!!! TTTTTATATTTGTATTTGGA+AGG - chr2_3:64310507-64310526 Msa0306050:intron 15.0%
!!! TTTTATATTTGTATTTGGAA+GGG - chr2_3:64310508-64310527 Msa0306050:intron 15.0%
!! AAACAAACAAAACAAAATAT+AGG + chr2_3:64310554-64310573 None:intergenic 15.0%
!!! AATAATATTTTGTGAACAAT+CGG + chr2_3:64309583-64309602 None:intergenic 15.0%
!!! ATTTACCATCATATTTTTAT+TGG - chr2_3:64309815-64309834 Msa0306050:intron 15.0%
!!! TTTTTATATTTGTATTTGGA+AGG - chr2_3:64310507-64310526 Msa0306050:intron 15.0%
!!! TTTTATATTTGTATTTGGAA+GGG - chr2_3:64310508-64310527 Msa0306050:intron 15.0%
!! AAACAAACAAAACAAAATAT+AGG + chr2_3:64310554-64310573 None:intergenic 15.0%
!! AATAATTCTGCTGAATATAA+TGG - chr2_3:64309310-64309329 Msa0306050:three_prime_UTR 20.0%
!!! TGAATATAATGGTCTAATTT+TGG - chr2_3:64309321-64309340 Msa0306050:three_prime_UTR 20.0%
!!! GAATATAATGGTCTAATTTT+GGG - chr2_3:64309322-64309341 Msa0306050:three_prime_UTR 20.0%
!!! AATATAATGGTCTAATTTTG+GGG - chr2_3:64309323-64309342 Msa0306050:three_prime_UTR 20.0%
!! TGTTCACAAAATATTATTAC+AGG - chr2_3:64309585-64309604 Msa0306050:intron 20.0%
!! ATGTTGTAATTATTACTACA+TGG - chr2_3:64309686-64309705 Msa0306050:intron 20.0%
!! CAAAACCAATAAAAATATGA+TGG + chr2_3:64309823-64309842 None:intergenic 20.0%
!! TTTATATTTGTATTTGGAAG+GGG - chr2_3:64310509-64310528 Msa0306050:intron 20.0%
!!! TTTTTTTTTTTTTTTGCAGC+CGG - chr2_3:64310574-64310593 Msa0306050:intron 20.0%
!! AATAATTCTGCTGAATATAA+TGG - chr2_3:64309310-64309329 Msa0306050:three_prime_UTR 20.0%
!!! TGAATATAATGGTCTAATTT+TGG - chr2_3:64309321-64309340 Msa0306050:three_prime_UTR 20.0%
!!! GAATATAATGGTCTAATTTT+GGG - chr2_3:64309322-64309341 Msa0306050:three_prime_UTR 20.0%
!!! AATATAATGGTCTAATTTTG+GGG - chr2_3:64309323-64309342 Msa0306050:three_prime_UTR 20.0%
!! TGTTCACAAAATATTATTAC+AGG - chr2_3:64309585-64309604 Msa0306050:intron 20.0%
!! ATGTTGTAATTATTACTACA+TGG - chr2_3:64309686-64309705 Msa0306050:intron 20.0%
!! CAAAACCAATAAAAATATGA+TGG + chr2_3:64309823-64309842 None:intergenic 20.0%
!! TTTATATTTGTATTTGGAAG+GGG - chr2_3:64310509-64310528 Msa0306050:intron 20.0%
!!! TTTTTTTTTTTTTTTGCAGC+CGG - chr2_3:64310574-64310593 Msa0306050:intron 20.0%
! ATAGTGCAAAAATGATTTGA+TGG + chr2_3:64309206-64309225 None:intergenic 25.0%
! ATGAGTTATAATAGACGTAA+GGG + chr2_3:64309428-64309447 None:intergenic 25.0%
!! AAATCACCGTGTAATATTTT+GGG + chr2_3:64309663-64309682 None:intergenic 25.0%
! ACACTATATATCAAGCAATT+AGG + chr2_3:64309758-64309777 None:intergenic 25.0%
! TATAGTGTTCCTATAGTAAT+TGG - chr2_3:64309770-64309789 Msa0306050:intron 25.0%
! CAAATCAAACCAATTACTAT+AGG + chr2_3:64309782-64309801 None:intergenic 25.0%
! TACAAAGGTTCCTTAAATTT+GGG + chr2_3:64309878-64309897 None:intergenic 25.0%
! TTACAAAGGTTCCTTAAATT+TGG + chr2_3:64309879-64309898 None:intergenic 25.0%
!!! TCATGTTTTTGTATTGTTGA+AGG - chr2_3:64310270-64310289 Msa0306050:intron 25.0%
!!! CATGTTTTTGTATTGTTGAA+GGG - chr2_3:64310271-64310290 Msa0306050:intron 25.0%
! AAACATCTTTCACTTGTATT+TGG - chr2_3:64310442-64310461 Msa0306050:intron 25.0%
!! CTATGTTAATTTTCCTGTTT+AGG - chr2_3:64310662-64310681 Msa0306050:CDS 25.0%
!!! ATTTTAAGTCACTTAAGCAA+TGG + chr2_3:64310799-64310818 None:intergenic 25.0%
! ATTGCTTAAGTGACTTAAAA+TGG - chr2_3:64310798-64310817 Msa0306050:intron 25.0%
! TAATATATTGTGCTATGACA+TGG - chr2_3:64310921-64310940 Msa0306050:CDS 25.0%
! ATAGTGCAAAAATGATTTGA+TGG + chr2_3:64309206-64309225 None:intergenic 25.0%
! ATGAGTTATAATAGACGTAA+GGG + chr2_3:64309428-64309447 None:intergenic 25.0%
!! AAATCACCGTGTAATATTTT+GGG + chr2_3:64309663-64309682 None:intergenic 25.0%
! ACACTATATATCAAGCAATT+AGG + chr2_3:64309758-64309777 None:intergenic 25.0%
! TATAGTGTTCCTATAGTAAT+TGG - chr2_3:64309770-64309789 Msa0306050:intron 25.0%
! CAAATCAAACCAATTACTAT+AGG + chr2_3:64309782-64309801 None:intergenic 25.0%
! TACAAAGGTTCCTTAAATTT+GGG + chr2_3:64309878-64309897 None:intergenic 25.0%
! TTACAAAGGTTCCTTAAATT+TGG + chr2_3:64309879-64309898 None:intergenic 25.0%
!!! TCATGTTTTTGTATTGTTGA+AGG - chr2_3:64310270-64310289 Msa0306050:intron 25.0%
!!! CATGTTTTTGTATTGTTGAA+GGG - chr2_3:64310271-64310290 Msa0306050:intron 25.0%
! AAACATCTTTCACTTGTATT+TGG - chr2_3:64310442-64310461 Msa0306050:intron 25.0%
!! CTATGTTAATTTTCCTGTTT+AGG - chr2_3:64310662-64310681 Msa0306050:CDS 25.0%
!!! ATTTTAAGTCACTTAAGCAA+TGG + chr2_3:64310799-64310818 None:intergenic 25.0%
! ATTGCTTAAGTGACTTAAAA+TGG - chr2_3:64310798-64310817 Msa0306050:intron 25.0%
! TAATATATTGTGCTATGACA+TGG - chr2_3:64310921-64310940 Msa0306050:CDS 25.0%
TCTTGTATCCTAGAATTTGA+TGG - chr2_3:64309100-64309119 Msa0306050:three_prime_UTR 30.0%
GATGAGTTATAATAGACGTA+AGG + chr2_3:64309429-64309448 None:intergenic 30.0%
TGTTAATGCATACACTGTAA+GGG + chr2_3:64309484-64309503 None:intergenic 30.0%
ATGTTAATGCATACACTGTA+AGG + chr2_3:64309485-64309504 None:intergenic 30.0%
CATCACTCATTTCATTCATA+TGG + chr2_3:64309542-64309561 None:intergenic 30.0%
TTGGTACCCAAAATATTACA+CGG - chr2_3:64309654-64309673 Msa0306050:intron 30.0%
! CAAATCACCGTGTAATATTT+TGG + chr2_3:64309664-64309683 None:intergenic 30.0%
TATATATCAAGCAATTAGGC+AGG + chr2_3:64309754-64309773 None:intergenic 30.0%
AAGTCAACAACCCAAATTTA+AGG - chr2_3:64309865-64309884 Msa0306050:intron 30.0%
TTTAAGGAACCTTTGTAATG+AGG - chr2_3:64309881-64309900 Msa0306050:intron 30.0%
! GTTTCTCTGGAAAACTTAAA+AGG - chr2_3:64310039-64310058 Msa0306050:intron 30.0%
CATAAACGATTCTCAAATGT+TGG + chr2_3:64310089-64310108 None:intergenic 30.0%
! GTTTTTCCTGTTAAAGCATA+TGG + chr2_3:64310170-64310189 None:intergenic 30.0%
!!! CAAGACTTTTGAGTTCTTAT+AGG - chr2_3:64310355-64310374 Msa0306050:intron 30.0%
!!! ATGTTTTTCTAAAGCAGCTT+TGG + chr2_3:64310428-64310447 None:intergenic 30.0%
! TGATGTTTGGATTTTATGCT+TGG - chr2_3:64310829-64310848 Msa0306050:intron 30.0%
AGAATTCAGAAACATTCACA+TGG + chr2_3:64310858-64310877 None:intergenic 30.0%
! TGTTCATGTCATTTCATAGA+TGG + chr2_3:64309066-64309085 None:intergenic 30.0%
TCTTGTATCCTAGAATTTGA+TGG - chr2_3:64309100-64309119 Msa0306050:three_prime_UTR 30.0%
GATGAGTTATAATAGACGTA+AGG + chr2_3:64309429-64309448 None:intergenic 30.0%
TGTTAATGCATACACTGTAA+GGG + chr2_3:64309484-64309503 None:intergenic 30.0%
ATGTTAATGCATACACTGTA+AGG + chr2_3:64309485-64309504 None:intergenic 30.0%
CATCACTCATTTCATTCATA+TGG + chr2_3:64309542-64309561 None:intergenic 30.0%
TTGGTACCCAAAATATTACA+CGG - chr2_3:64309654-64309673 Msa0306050:intron 30.0%
! CAAATCACCGTGTAATATTT+TGG + chr2_3:64309664-64309683 None:intergenic 30.0%
TATATATCAAGCAATTAGGC+AGG + chr2_3:64309754-64309773 None:intergenic 30.0%
AAGTCAACAACCCAAATTTA+AGG - chr2_3:64309865-64309884 Msa0306050:intron 30.0%
TTTAAGGAACCTTTGTAATG+AGG - chr2_3:64309881-64309900 Msa0306050:intron 30.0%
! GTTTCTCTGGAAAACTTAAA+AGG - chr2_3:64310039-64310058 Msa0306050:intron 30.0%
CATAAACGATTCTCAAATGT+TGG + chr2_3:64310089-64310108 None:intergenic 30.0%
! GTTTTTCCTGTTAAAGCATA+TGG + chr2_3:64310170-64310189 None:intergenic 30.0%
!!! CAAGACTTTTGAGTTCTTAT+AGG - chr2_3:64310355-64310374 Msa0306050:intron 30.0%
!!! ATGTTTTTCTAAAGCAGCTT+TGG + chr2_3:64310428-64310447 None:intergenic 30.0%
! TGATGTTTGGATTTTATGCT+TGG - chr2_3:64310829-64310848 Msa0306050:intron 30.0%
AGAATTCAGAAACATTCACA+TGG + chr2_3:64310858-64310877 None:intergenic 30.0%
AACCCTTTATGTCTCTTTGT+AGG - chr2_3:64309252-64309271 Msa0306050:three_prime_UTR 35.0%
GACCTACAAAGAGACATAAA+GGG + chr2_3:64309257-64309276 None:intergenic 35.0%
! TAAATTCCTTGTTTGCAAGC+AGG - chr2_3:64309452-64309471 Msa0306050:intron 35.0%
!! GTGTAATATTTTGGGTACCA+AGG + chr2_3:64309655-64309674 None:intergenic 35.0%
!!! ATTGGTTTTGCAACAGTTTG+AGG - chr2_3:64309833-64309852 Msa0306050:intron 35.0%
!!! TTGGTTTTGCAACAGTTTGA+GGG - chr2_3:64309834-64309853 Msa0306050:intron 35.0%
GGTATACACTTTGTCTCATT+TGG - chr2_3:64309956-64309975 Msa0306050:intron 35.0%
CTGGAAAACTTAAAAGGTTG+AGG - chr2_3:64310045-64310064 Msa0306050:intron 35.0%
! GAGAGGCATGCTAATTAAAA+AGG + chr2_3:64310130-64310149 None:intergenic 35.0%
TCACTTGTATTTGGTTGCAA+TGG - chr2_3:64310451-64310470 Msa0306050:intron 35.0%
GTAGTAATATTCAACTTCGC+CGG + chr2_3:64310596-64310615 None:intergenic 35.0%
ATGCCACTGTCAAAAACTAT+TGG - chr2_3:64310636-64310655 Msa0306050:CDS 35.0%
! TTGCCAATAGTTTTTGACAG+TGG + chr2_3:64310642-64310661 None:intergenic 35.0%
ATGGTGAGATATACACATCA+TGG + chr2_3:64310780-64310799 None:intergenic 35.0%
!! AATGGTGTTGCTTTGATGTT+TGG - chr2_3:64310816-64310835 Msa0306050:intron 35.0%
TGTCTTCATCTGTCTTCATT+TGG - chr2_3:64310884-64310903 Msa0306050:CDS 35.0%
AACCCTTTATGTCTCTTTGT+AGG - chr2_3:64309252-64309271 Msa0306050:three_prime_UTR 35.0%
GACCTACAAAGAGACATAAA+GGG + chr2_3:64309257-64309276 None:intergenic 35.0%
! TAAATTCCTTGTTTGCAAGC+AGG - chr2_3:64309452-64309471 Msa0306050:intron 35.0%
!! GTGTAATATTTTGGGTACCA+AGG + chr2_3:64309655-64309674 None:intergenic 35.0%
!!! ATTGGTTTTGCAACAGTTTG+AGG - chr2_3:64309833-64309852 Msa0306050:intron 35.0%
!!! TTGGTTTTGCAACAGTTTGA+GGG - chr2_3:64309834-64309853 Msa0306050:intron 35.0%
GGTATACACTTTGTCTCATT+TGG - chr2_3:64309956-64309975 Msa0306050:intron 35.0%
CTGGAAAACTTAAAAGGTTG+AGG - chr2_3:64310045-64310064 Msa0306050:intron 35.0%
! GAGAGGCATGCTAATTAAAA+AGG + chr2_3:64310130-64310149 None:intergenic 35.0%
TCACTTGTATTTGGTTGCAA+TGG - chr2_3:64310451-64310470 Msa0306050:intron 35.0%
ATGCCACTGTCAAAAACTAT+TGG - chr2_3:64310636-64310655 Msa0306050:CDS 35.0%
! TTGCCAATAGTTTTTGACAG+TGG + chr2_3:64310642-64310661 None:intergenic 35.0%
ATGGTGAGATATACACATCA+TGG + chr2_3:64310780-64310799 None:intergenic 35.0%
!! AATGGTGTTGCTTTGATGTT+TGG - chr2_3:64310816-64310835 Msa0306050:intron 35.0%
TGTCTTCATCTGTCTTCATT+TGG - chr2_3:64310884-64310903 Msa0306050:CDS 35.0%
TGGATGCTCCATCAAATTCT+AGG + chr2_3:64309111-64309130 None:intergenic 40.0%
GGACCTACAAAGAGACATAA+AGG + chr2_3:64309258-64309277 None:intergenic 40.0%
TATGTCTCTTTGTAGGTCCA+TGG - chr2_3:64309259-64309278 Msa0306050:three_prime_UTR 40.0%
CATGAACATGCTCATATCCT+TGG + chr2_3:64309366-64309385 None:intergenic 40.0%
AACTAACCTGCTTGCAAACA+AGG + chr2_3:64309405-64309424 None:intergenic 40.0%
TCTGCATTAACAGCAATGCA+AGG - chr2_3:64309711-64309730 Msa0306050:CDS 40.0%
!! ATTTGGGTTGTTGACTTTCC+AGG + chr2_3:64309862-64309881 None:intergenic 40.0%
TGAGACAAAGTGTATACCCT+AGG + chr2_3:64309954-64309973 None:intergenic 40.0%
ACCTTGATTGTGTGTTTCTC+TGG - chr2_3:64310026-64310045 Msa0306050:intron 40.0%
TCCAGAGAAACACACAATCA+AGG + chr2_3:64310030-64310049 None:intergenic 40.0%
! TAGTTTTTGACAGTGGCATG+CGG + chr2_3:64310635-64310654 None:intergenic 40.0%
TGCCTACTGTTCAGCATTTA+GGG - chr2_3:64310688-64310707 Msa0306050:CDS 40.0%
TTAACACCATCCAGTCATGT+TGG + chr2_3:64310947-64310966 None:intergenic 40.0%
TGGATGCTCCATCAAATTCT+AGG + chr2_3:64309111-64309130 None:intergenic 40.0%
GGACCTACAAAGAGACATAA+AGG + chr2_3:64309258-64309277 None:intergenic 40.0%
TATGTCTCTTTGTAGGTCCA+TGG - chr2_3:64309259-64309278 Msa0306050:three_prime_UTR 40.0%
CATGAACATGCTCATATCCT+TGG + chr2_3:64309366-64309385 None:intergenic 40.0%
AACTAACCTGCTTGCAAACA+AGG + chr2_3:64309405-64309424 None:intergenic 40.0%
TCTGCATTAACAGCAATGCA+AGG - chr2_3:64309711-64309730 Msa0306050:CDS 40.0%
!! ATTTGGGTTGTTGACTTTCC+AGG + chr2_3:64309862-64309881 None:intergenic 40.0%
TGAGACAAAGTGTATACCCT+AGG + chr2_3:64309954-64309973 None:intergenic 40.0%
ACCTTGATTGTGTGTTTCTC+TGG - chr2_3:64310026-64310045 Msa0306050:intron 40.0%
TCCAGAGAAACACACAATCA+AGG + chr2_3:64310030-64310049 None:intergenic 40.0%
! TAGTTTTTGACAGTGGCATG+CGG + chr2_3:64310635-64310654 None:intergenic 40.0%
TGCCTACTGTTCAGCATTTA+GGG - chr2_3:64310688-64310707 Msa0306050:CDS 40.0%
TTAACACCATCCAGTCATGT+TGG + chr2_3:64310947-64310966 None:intergenic 40.0%
! GAATTTGATGGAGCATCCAG+TGG - chr2_3:64309112-64309131 Msa0306050:three_prime_UTR 45.0%
!!! TTTTGGGGCTGAATGAAGCT+AGG - chr2_3:64309338-64309357 Msa0306050:three_prime_UTR 45.0%
CTGAATGAAGCTAGGAACCA+AGG - chr2_3:64309346-64309365 Msa0306050:three_prime_UTR 45.0%
TATGAGCATGTTCATGTCCG+AGG - chr2_3:64309370-64309389 Msa0306050:three_prime_UTR 45.0%
AAACAAGCTGAGAGTCACCT+CGG + chr2_3:64309390-64309409 None:intergenic 45.0%
GAACCTTTGTAATGAGGCTG+TGG - chr2_3:64309887-64309906 Msa0306050:intron 45.0%
! TCAGTGTCGAACATGTTCCT+AGG - chr2_3:64309934-64309953 Msa0306050:intron 45.0%
! CAGTGTCGAACATGTTCCTA+GGG - chr2_3:64309935-64309954 Msa0306050:intron 45.0%
ATCGCGTGACTTATGTAGAG+AGG + chr2_3:64310147-64310166 None:intergenic 45.0%
CGCGATCCATATGCTTTAAC+AGG - chr2_3:64310161-64310180 Msa0306050:CDS 45.0%
GCAAATCGCGGCAAAAATCT+TGG - chr2_3:64310319-64310338 Msa0306050:intron 45.0%
AATCGCGGCAAAAATCTTGG+AGG - chr2_3:64310322-64310341 Msa0306050:intron 45.0%
ACAGTAGGCACAACCTAAAC+AGG + chr2_3:64310678-64310697 None:intergenic 45.0%
GTGCCTACTGTTCAGCATTT+AGG - chr2_3:64310687-64310706 Msa0306050:CDS 45.0%
GCCTACTGTTCAGCATTTAG+GGG - chr2_3:64310689-64310708 Msa0306050:CDS 45.0%
CCTACTGTTCAGCATTTAGG+GGG - chr2_3:64310690-64310709 Msa0306050:CDS 45.0%
TATGACATGGCCAACATGAC+TGG - chr2_3:64310934-64310953 Msa0306050:CDS 45.0%
! GAATTTGATGGAGCATCCAG+TGG - chr2_3:64309112-64309131 Msa0306050:three_prime_UTR 45.0%
!!! TTTTGGGGCTGAATGAAGCT+AGG - chr2_3:64309338-64309357 Msa0306050:three_prime_UTR 45.0%
CTGAATGAAGCTAGGAACCA+AGG - chr2_3:64309346-64309365 Msa0306050:three_prime_UTR 45.0%
TATGAGCATGTTCATGTCCG+AGG - chr2_3:64309370-64309389 Msa0306050:three_prime_UTR 45.0%
AAACAAGCTGAGAGTCACCT+CGG + chr2_3:64309390-64309409 None:intergenic 45.0%
GAACCTTTGTAATGAGGCTG+TGG - chr2_3:64309887-64309906 Msa0306050:intron 45.0%
! TCAGTGTCGAACATGTTCCT+AGG - chr2_3:64309934-64309953 Msa0306050:intron 45.0%
! CAGTGTCGAACATGTTCCTA+GGG - chr2_3:64309935-64309954 Msa0306050:intron 45.0%
ATCGCGTGACTTATGTAGAG+AGG + chr2_3:64310147-64310166 None:intergenic 45.0%
CGCGATCCATATGCTTTAAC+AGG - chr2_3:64310161-64310180 Msa0306050:CDS 45.0%
GCAAATCGCGGCAAAAATCT+TGG - chr2_3:64310319-64310338 Msa0306050:intron 45.0%
AATCGCGGCAAAAATCTTGG+AGG - chr2_3:64310322-64310341 Msa0306050:intron 45.0%
ACAGTAGGCACAACCTAAAC+AGG + chr2_3:64310678-64310697 None:intergenic 45.0%
GTGCCTACTGTTCAGCATTT+AGG - chr2_3:64310687-64310706 Msa0306050:CDS 45.0%
GCCTACTGTTCAGCATTTAG+GGG - chr2_3:64310689-64310708 Msa0306050:CDS 45.0%
CCTACTGTTCAGCATTTAGG+GGG - chr2_3:64310690-64310709 Msa0306050:CDS 45.0%
TATGACATGGCCAACATGAC+TGG - chr2_3:64310934-64310953 Msa0306050:CDS 45.0%
!!! ATTTTTTTTATATTTGTATT+TGG - chr2_3:64310503-64310522 Msa0306050:intron 5.0%
!!! ATTTTTTTTATATTTGTATT+TGG - chr2_3:64310503-64310522 Msa0306050:intron 5.0%
CATCCAGTGGAAATCCTGGA+AGG - chr2_3:64309125-64309144 Msa0306050:three_prime_UTR 50.0%
AGACCTTCCAGGATTTCCAC+TGG + chr2_3:64309131-64309150 None:intergenic 50.0%
AGTGGAAATCCTGGAAGGTC+TGG - chr2_3:64309130-64309149 Msa0306050:three_prime_UTR 50.0%
CTGTTCTGCGTTCTGCTGAT+GGG - chr2_3:64309161-64309180 Msa0306050:three_prime_UTR 50.0%
!! TTGTTTGAGTGCCGAGTCCT+TGG + chr2_3:64309291-64309310 None:intergenic 50.0%
! CTCTCAGCTTGTTTGCAAGC+AGG - chr2_3:64309396-64309415 Msa0306050:three_prime_UTR 50.0%
!! GTTTTCCTATGCGTCGACCT+TGG - chr2_3:64309635-64309654 Msa0306050:intron 50.0%
TGCAACAGTTTGAGGGTTCC+TGG - chr2_3:64309841-64309860 Msa0306050:intron 50.0%
GCTCCACAGCCTCATTACAA+AGG + chr2_3:64309893-64309912 None:intergenic 50.0%
GCTGATGCTCAAGCAAATCG+CGG - chr2_3:64310307-64310326 Msa0306050:intron 50.0%
CCCCCTAAATGCTGAACAGT+AGG + chr2_3:64310693-64310712 None:intergenic 50.0%
ACATGGCCAACATGACTGGA+TGG - chr2_3:64310938-64310957 Msa0306050:CDS 50.0%
CATCCAGTGGAAATCCTGGA+AGG - chr2_3:64309125-64309144 Msa0306050:three_prime_UTR 50.0%
AGACCTTCCAGGATTTCCAC+TGG + chr2_3:64309131-64309150 None:intergenic 50.0%
AGTGGAAATCCTGGAAGGTC+TGG - chr2_3:64309130-64309149 Msa0306050:three_prime_UTR 50.0%
CTGTTCTGCGTTCTGCTGAT+GGG - chr2_3:64309161-64309180 Msa0306050:three_prime_UTR 50.0%
!! TTGTTTGAGTGCCGAGTCCT+TGG + chr2_3:64309291-64309310 None:intergenic 50.0%
! CTCTCAGCTTGTTTGCAAGC+AGG - chr2_3:64309396-64309415 Msa0306050:three_prime_UTR 50.0%
!! GTTTTCCTATGCGTCGACCT+TGG - chr2_3:64309635-64309654 Msa0306050:intron 50.0%
TGCAACAGTTTGAGGGTTCC+TGG - chr2_3:64309841-64309860 Msa0306050:intron 50.0%
GCTCCACAGCCTCATTACAA+AGG + chr2_3:64309893-64309912 None:intergenic 50.0%
GCTGATGCTCAAGCAAATCG+CGG - chr2_3:64310307-64310326 Msa0306050:intron 50.0%
CCCCCTAAATGCTGAACAGT+AGG + chr2_3:64310693-64310712 None:intergenic 50.0%
ACATGGCCAACATGACTGGA+TGG - chr2_3:64310938-64310957 Msa0306050:CDS 50.0%
! GGAGCATCCAGTGGAAATCC+TGG - chr2_3:64309121-64309140 Msa0306050:three_prime_UTR 55.0%
AATCCTGGAAGGTCTGGTGC+AGG - chr2_3:64309136-64309155 Msa0306050:three_prime_UTR 55.0%
GCTGTTCTGCGTTCTGCTGA+TGG - chr2_3:64309160-64309179 Msa0306050:three_prime_UTR 55.0%
TGCGTTCTGCTGATGGGAGT+CGG - chr2_3:64309167-64309186 Msa0306050:three_prime_UTR 55.0%
! GGAGCATCCAGTGGAAATCC+TGG - chr2_3:64309121-64309140 Msa0306050:three_prime_UTR 55.0%
AATCCTGGAAGGTCTGGTGC+AGG - chr2_3:64309136-64309155 Msa0306050:three_prime_UTR 55.0%
GCTGTTCTGCGTTCTGCTGA+TGG - chr2_3:64309160-64309179 Msa0306050:three_prime_UTR 55.0%
TGCGTTCTGCTGATGGGAGT+CGG - chr2_3:64309167-64309186 Msa0306050:three_prime_UTR 55.0%
GCGTTCTGCTGATGGGAGTC+GGG - chr2_3:64309168-64309187 Msa0306050:three_prime_UTR 60.0%
TAGGTCCATGGCGTCAGCCA+AGG - chr2_3:64309271-64309290 Msa0306050:three_prime_UTR 60.0%
CATGGCGTCAGCCAAGGACT+CGG - chr2_3:64309277-64309296 Msa0306050:three_prime_UTR 60.0%
GGGTACCAAGGTCGACGCAT+AGG + chr2_3:64309643-64309662 None:intergenic 60.0%
GCGTTCTGCTGATGGGAGTC+GGG - chr2_3:64309168-64309187 Msa0306050:three_prime_UTR 60.0%
TAGGTCCATGGCGTCAGCCA+AGG - chr2_3:64309271-64309290 Msa0306050:three_prime_UTR 60.0%
CATGGCGTCAGCCAAGGACT+CGG - chr2_3:64309277-64309296 Msa0306050:three_prime_UTR 60.0%
GGGTACCAAGGTCGACGCAT+AGG + chr2_3:64309643-64309662 None:intergenic 60.0%
GCTCCTGCACCAGACCTTCC+AGG + chr2_3:64309142-64309161 None:intergenic 65.0%
CGAGTCCTTGGCTGACGCCA+TGG + chr2_3:64309279-64309298 None:intergenic 65.0%
GCTCCTGCACCAGACCTTCC+AGG + chr2_3:64309142-64309161 None:intergenic 65.0%
CGAGTCCTTGGCTGACGCCA+TGG + chr2_3:64309279-64309298 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 64309065 64310976 64309065 ID=Msa0306050;Name=Msa0306050
chr2_3 mRNA 64309065 64310976 64309065 ID=Msa0306050-mRNA-1;Parent=Msa0306050;Name=Msa0306050-mRNA-1;_AED=0.00;_eAED=0.00;_QI=14|1|1|1|1|1|5|358|146
chr2_3 exon 64309065 64309448 64309065 ID=Msa0306050-mRNA-1:exon:21501;Parent=Msa0306050-mRNA-1
chr2_3 exon 64309698 64309749 64309698 ID=Msa0306050-mRNA-1:exon:21500;Parent=Msa0306050-mRNA-1
chr2_3 exon 64310085 64310192 64310085 ID=Msa0306050-mRNA-1:exon:21499;Parent=Msa0306050-mRNA-1
chr2_3 exon 64310624 64310767 64310624 ID=Msa0306050-mRNA-1:exon:21498;Parent=Msa0306050-mRNA-1
chr2_3 exon 64310852 64310976 64310852 ID=Msa0306050-mRNA-1:exon:21497;Parent=Msa0306050-mRNA-1
chr2_3 five_prime_UTR 64310963 64310976 64310963 ID=Msa0306050-mRNA-1:five_prime_utr;Parent=Msa0306050-mRNA-1
chr2_3 CDS 64310852 64310962 64310852 ID=Msa0306050-mRNA-1:cds;Parent=Msa0306050-mRNA-1
chr2_3 CDS 64310624 64310767 64310624 ID=Msa0306050-mRNA-1:cds;Parent=Msa0306050-mRNA-1
chr2_3 CDS 64310085 64310192 64310085 ID=Msa0306050-mRNA-1:cds;Parent=Msa0306050-mRNA-1
chr2_3 CDS 64309698 64309749 64309698 ID=Msa0306050-mRNA-1:cds;Parent=Msa0306050-mRNA-1
chr2_3 CDS 64309423 64309448 64309423 ID=Msa0306050-mRNA-1:cds;Parent=Msa0306050-mRNA-1
chr2_3 three_prime_UTR 64309065 64309422 64309065 ID=Msa0306050-mRNA-1:three_prime_utr;Parent=Msa0306050-mRNA-1
Gene Sequence

>Msa0306050

ATGAACAAAATCTTGCAGCGTTCTTGTATCCTAGAATTTGATGGAGCATCCAGTGGAAATCCTGGAAGGTCTGGTGCAGGAGCTGTTCTGCGTTCTGCTGATGGGAGTCGGGTCCATGGCGTCAGCCAAGGACTCGGCACTCAAACAAATAATTCTGCTGAATATAATGGTCTAATTTTGGGGCTGAATGAAGCTAGGAACCAAGGATATGAGCATGTTCATGTCCGAGGTGACTCTCAGCTTGTTTGCAAGCAGTTTGAGGGTTCCTGGAAAGTCAACAACCCAAATTTAAGGAACCTTTGTAATGAGGCTGTGGAGCTGAAGAGTAACTTCAAGTCAGTCAGTGTCGAACATGTTCCTAGGGGTTCTAACTCTGCAGCTGATGCTCAAGCAAATCGCGGCAAAAATCTTGGAGCCGGCGAAGTTGAATATTACTACTGA
Protein sequence

>Msa0306050

MNKILQRSCILEFDGASSGNPGRSGAGAVLRSADGSRVHGVSQGLGTQTNNSAEYNGLILGLNEARNQGYEHVHVRGDSQLVCKQFEGSWKVNNPNLRNLCNEAVELKSNFKSVSVEHVPRGSNSAADAQANRGKNLGAGEVEYYY*