AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1421290

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1421290 MtrunA17_Chr2g0325301 99.291 141 1 0 6 146 8 148 5.25e-101 286
Msa1421290 MtrunA17_Chr6g0451421 58.696 138 57 0 6 143 6 143 1.24e-53 166
Msa1421290 MtrunA17_Chr6g0451411 59.559 136 55 0 8 143 176 311 4.85e-52 167
Msa1421290 MtrunA17_Chr2g0329701 51.429 140 68 0 7 146 233 372 6.36e-46 153
Msa1421290 MtrunA17_Chr7g0264421 51.825 137 66 0 7 143 203 339 1.17e-45 151
Msa1421290 MtrunA17_Chr6g0451441 46.575 146 70 1 6 143 318 463 1.54e-35 127
Msa1421290 MtrunA17_Chr6g0451451 50.000 82 41 0 62 143 1 82 5.83e-25 91.3
Msa1421290 MtrunA17_Chr1g0158081 37.190 121 74 1 11 131 1209 1327 3.24e-16 74.3
Msa1421290 MtrunA17_Chr3g0110591 36.364 121 75 1 11 131 25 143 3.66e-15 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1421290 AT3G01410.2 50.000 136 68 0 8 143 154 289 3.37e-45 149
Msa1421290 AT3G01410.1 50.000 136 68 0 8 143 154 289 3.37e-45 149
Msa1421290 AT5G51080.3 53.285 137 64 0 7 143 121 257 4.79e-45 148
Msa1421290 AT5G51080.1 53.676 136 63 0 8 143 185 320 2.57e-44 148
Msa1421290 AT5G51080.2 53.676 136 63 0 8 143 185 320 2.57e-44 148
Msa1421290 AT1G24090.1 52.143 140 67 0 4 143 212 351 1.92e-41 141

Find 52 sgRNAs with CRISPR-Local

Find 233 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACAAAGGTTCCTTAAATT+TGG 0.088756 tig0024922:+43095 None:intergenic
TGAATATAATGGTCTAATTT+TGG 0.114690 tig0024922:-43650 Msa1421290:CDS
TGCCTACTTGTTCAGCATTT+AGG 0.169325 tig0024922:-42282 Msa1421290:three_prime_UTR
ATTGCTTAAGTGACTTAAAA+TGG 0.233393 tig0024922:-42171 Msa1421290:three_prime_UTR
GCCTACTTGTTCAGCATTTA+GGG 0.238217 tig0024922:-42281 Msa1421290:three_prime_UTR
TGTCTTCATCTGTCTTCATT+TGG 0.308569 tig0024922:-42084 Msa1421290:three_prime_UTR
TACAAAGGTTCCTTAAATTT+GGG 0.313196 tig0024922:+43096 None:intergenic
TGCAACAGTTTGAGGGTTCC+TGG 0.323750 tig0024922:-43130 Msa1421290:intron
TGGATGCTCCATCAAATTCT+AGG 0.329321 tig0024922:+43863 None:intergenic
AAGTCAACAACCCAAATTTA+AGG 0.331211 tig0024922:-43106 Msa1421290:CDS
ATTTGGGTTGTTGACTTTCC+AGG 0.336100 tig0024922:+43112 None:intergenic
AATGGTGTTGCTTTGATGTT+TGG 0.346803 tig0024922:-42153 Msa1421290:three_prime_UTR
TCTTGTATCCTAGAATTTGA+TGG 0.357172 tig0024922:-43871 Msa1421290:CDS
AATAATTCTGCTGAATATAA+TGG 0.390357 tig0024922:-43661 Msa1421290:CDS
CAAGTAGGCACAACCTAAAC+AGG 0.396785 tig0024922:+42295 None:intergenic
GCTCCTGCACCAGACCTTCC+AGG 0.402463 tig0024922:+43832 None:intergenic
TGCGTTCTGCTGATGGGAGT+CGG 0.407658 tig0024922:-43804 Msa1421290:CDS
CTCTCAGCTTGTTTGCAAGC+AGG 0.424209 tig0024922:-43575 Msa1421290:intron
TTTAAGGAACCTTTGTAATG+AGG 0.445867 tig0024922:-43090 Msa1421290:CDS
GCTGTTCTGCGTTCTGCTGA+TGG 0.448826 tig0024922:-43811 Msa1421290:CDS
TATGTCTCTTTGTAGGTCCA+TGG 0.454685 tig0024922:-43712 Msa1421290:intron
GGAGCATCCAGTGGAAATCC+TGG 0.459158 tig0024922:-43850 Msa1421290:CDS
GCGTTCTGCTGATGGGAGTC+GGG 0.465577 tig0024922:-43803 Msa1421290:intron
AGTGGAAATCCTGGAAGGTC+TGG 0.475520 tig0024922:-43841 Msa1421290:CDS
CTGTTCTGCGTTCTGCTGAT+GGG 0.478692 tig0024922:-43810 Msa1421290:CDS
TTGTTTGAGTGCCGAGTCCT+TGG 0.487817 tig0024922:+43683 None:intergenic
TCAGTGTCGAACATGTTCCT+AGG 0.502100 tig0024922:-43037 Msa1421290:CDS
CATGAACATGCTCATATCCT+TGG 0.505728 tig0024922:+43608 None:intergenic
CATGGCGTCAGCCAAGGACT+CGG 0.511067 tig0024922:-43694 Msa1421290:CDS
TATGACATGGCCAACATGAC+TGG 0.542678 tig0024922:-42034 Msa1421290:three_prime_UTR
CCTACTTGTTCAGCATTTAG+GGG 0.543120 tig0024922:-42280 Msa1421290:three_prime_UTR
CTACTTGTTCAGCATTTAGG+GGG 0.546015 tig0024922:-42279 Msa1421290:three_prime_UTR
CGAGTCCTTGGCTGACGCCA+TGG 0.551131 tig0024922:+43695 None:intergenic
TAGGTCCATGGCGTCAGCCA+AGG 0.555171 tig0024922:-43700 Msa1421290:CDS
AGACCTTCCAGGATTTCCAC+TGG 0.556723 tig0024922:+43843 None:intergenic
GAACCTTTGTAATGAGGCTG+TGG 0.560005 tig0024922:-43084 Msa1421290:CDS
AATCCTGGAAGGTCTGGTGC+AGG 0.565510 tig0024922:-43835 Msa1421290:CDS
GTAGTAATATTCAACTTCGC+CGG 0.574495 tig0024922:+42377 None:intergenic
ATGGTGAGATATACACATCA+TGG 0.586235 tig0024922:+42192 None:intergenic
CAGTGTCGAACATGTTCCTA+GGG 0.587867 tig0024922:-43036 Msa1421290:intron
GCTCCACAGCCTCATTACAA+AGG 0.619284 tig0024922:+43081 None:intergenic
TTAACACCATCCAGTCATGT+TGG 0.629124 tig0024922:+42024 None:intergenic
CTGAATGAAGCTAGGAACCA+AGG 0.629738 tig0024922:-43625 Msa1421290:CDS
GAATTTGATGGAGCATCCAG+TGG 0.630666 tig0024922:-43859 Msa1421290:CDS
TATGAGCATGTTCATGTCCG+AGG 0.645245 tig0024922:-43601 Msa1421290:CDS
GCTGATGCTCAAGCAAATCG+CGG 0.646058 tig0024922:-42664 Msa1421290:CDS
CCCCTAAATGCTGAACAAGT+AGG 0.647058 tig0024922:+42280 None:intergenic
ACATGGCCAACATGACTGGA+TGG 0.655888 tig0024922:-42030 Msa1421290:three_prime_UTR
CATCCAGTGGAAATCCTGGA+AGG 0.657104 tig0024922:-43846 Msa1421290:CDS
TAATATATTGTGCTATGACA+TGG 0.692388 tig0024922:-42047 Msa1421290:three_prime_UTR
AAACAAGCTGAGAGTCACCT+CGG 0.700596 tig0024922:+43584 None:intergenic
GAAATTCAGAAACATTCACA+TGG 0.721736 tig0024922:+42114 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAATATTTTGTGAACAAT+CGG + tig0024922:42532-42551 None:intergenic 15.0%
!!! ATTTACCATCATATTTTTAT+TGG - tig0024922:42764-42783 Msa1421290:intron 15.0%
!!! TTTTTATATTTGTATTTGGA+AGG - tig0024922:43457-43476 Msa1421290:intron 15.0%
!!! TTTTATATTTGTATTTGGAA+GGG - tig0024922:43458-43477 Msa1421290:intron 15.0%
!! AACAAAACAAAACAAAATAT+AGG + tig0024922:43504-43523 None:intergenic 15.0%
!!! AATAATATTTTGTGAACAAT+CGG + tig0024922:42532-42551 None:intergenic 15.0%
!!! ATTTACCATCATATTTTTAT+TGG - tig0024922:42764-42783 Msa1421290:intron 15.0%
!!! TTTTTATATTTGTATTTGGA+AGG - tig0024922:43457-43476 Msa1421290:intron 15.0%
!!! TTTTATATTTGTATTTGGAA+GGG - tig0024922:43458-43477 Msa1421290:intron 15.0%
!! AACAAAACAAAACAAAATAT+AGG + tig0024922:43504-43523 None:intergenic 15.0%
!! AATAATTCTGCTGAATATAA+TGG - tig0024922:42259-42278 Msa1421290:three_prime_UTR 20.0%
!!! TGAATATAATGGTCTAATTT+TGG - tig0024922:42270-42289 Msa1421290:three_prime_UTR 20.0%
!!! GAATATAATGGTCTAATTTT+GGG - tig0024922:42271-42290 Msa1421290:three_prime_UTR 20.0%
!!! AATATAATGGTCTAATTTTG+GGG - tig0024922:42272-42291 Msa1421290:three_prime_UTR 20.0%
!! TGTTCACAAAATATTATTAC+AGG - tig0024922:42534-42553 Msa1421290:intron 20.0%
!! ATGTTGTAATTATTACTACA+TGG - tig0024922:42635-42654 Msa1421290:intron 20.0%
!! CAAAACCAATAAAAATATGA+TGG + tig0024922:42772-42791 None:intergenic 20.0%
!! TTGTACACAATATTTCTTAA+TGG + tig0024922:43434-43453 None:intergenic 20.0%
!! TTTATATTTGTATTTGGAAG+GGG - tig0024922:43459-43478 Msa1421290:intron 20.0%
!!! TTTTTTTTTTTTTTTGCAGC+CGG - tig0024922:43524-43543 Msa1421290:intron 20.0%
!! AATAATTCTGCTGAATATAA+TGG - tig0024922:42259-42278 Msa1421290:three_prime_UTR 20.0%
!!! TGAATATAATGGTCTAATTT+TGG - tig0024922:42270-42289 Msa1421290:three_prime_UTR 20.0%
!!! GAATATAATGGTCTAATTTT+GGG - tig0024922:42271-42290 Msa1421290:three_prime_UTR 20.0%
!!! AATATAATGGTCTAATTTTG+GGG - tig0024922:42272-42291 Msa1421290:three_prime_UTR 20.0%
!! TGTTCACAAAATATTATTAC+AGG - tig0024922:42534-42553 Msa1421290:intron 20.0%
!! ATGTTGTAATTATTACTACA+TGG - tig0024922:42635-42654 Msa1421290:intron 20.0%
!! CAAAACCAATAAAAATATGA+TGG + tig0024922:42772-42791 None:intergenic 20.0%
!! TTGTACACAATATTTCTTAA+TGG + tig0024922:43434-43453 None:intergenic 20.0%
!! TTTATATTTGTATTTGGAAG+GGG - tig0024922:43459-43478 Msa1421290:intron 20.0%
!!! TTTTTTTTTTTTTTTGCAGC+CGG - tig0024922:43524-43543 Msa1421290:intron 20.0%
! ATAGTGCAAAAATGATTTGA+TGG + tig0024922:42155-42174 None:intergenic 25.0%
! ATGAGTTATAATAGACGTAA+GGG + tig0024922:42377-42396 None:intergenic 25.0%
!! AAATCACCGTGTAATATTTT+GGG + tig0024922:42612-42631 None:intergenic 25.0%
! ACACTATATATCAAGCAATT+AGG + tig0024922:42707-42726 None:intergenic 25.0%
! TATAGTGTTCCTATAGTAAT+TGG - tig0024922:42719-42738 Msa1421290:intron 25.0%
! CAAATCAAACCAATTACTAT+AGG + tig0024922:42731-42750 None:intergenic 25.0%
! TACAAAGGTTCCTTAAATTT+GGG + tig0024922:42827-42846 None:intergenic 25.0%
! TTACAAAGGTTCCTTAAATT+TGG + tig0024922:42828-42847 None:intergenic 25.0%
!!! TCATGTTTTTGTATTGTTGA+AGG - tig0024922:43219-43238 Msa1421290:intron 25.0%
!!! CATGTTTTTGTATTGTTGAA+GGG - tig0024922:43220-43239 Msa1421290:intron 25.0%
! AAACATCTTTCACTTGTATT+TGG - tig0024922:43391-43410 Msa1421290:intron 25.0%
!! CTATGTTAATTTTCCTGTTT+AGG - tig0024922:43612-43631 Msa1421290:CDS 25.0%
!!! ATTTTAAGTCACTTAAGCAA+TGG + tig0024922:43750-43769 None:intergenic 25.0%
! ATTGCTTAAGTGACTTAAAA+TGG - tig0024922:43749-43768 Msa1421290:intron 25.0%
! TAATATATTGTGCTATGACA+TGG - tig0024922:43873-43892 Msa1421290:CDS 25.0%
! ATAGTGCAAAAATGATTTGA+TGG + tig0024922:42155-42174 None:intergenic 25.0%
! ATGAGTTATAATAGACGTAA+GGG + tig0024922:42377-42396 None:intergenic 25.0%
!! AAATCACCGTGTAATATTTT+GGG + tig0024922:42612-42631 None:intergenic 25.0%
! ACACTATATATCAAGCAATT+AGG + tig0024922:42707-42726 None:intergenic 25.0%
! TATAGTGTTCCTATAGTAAT+TGG - tig0024922:42719-42738 Msa1421290:intron 25.0%
! CAAATCAAACCAATTACTAT+AGG + tig0024922:42731-42750 None:intergenic 25.0%
! TACAAAGGTTCCTTAAATTT+GGG + tig0024922:42827-42846 None:intergenic 25.0%
! TTACAAAGGTTCCTTAAATT+TGG + tig0024922:42828-42847 None:intergenic 25.0%
!!! TCATGTTTTTGTATTGTTGA+AGG - tig0024922:43219-43238 Msa1421290:intron 25.0%
!!! CATGTTTTTGTATTGTTGAA+GGG - tig0024922:43220-43239 Msa1421290:intron 25.0%
! AAACATCTTTCACTTGTATT+TGG - tig0024922:43391-43410 Msa1421290:intron 25.0%
!! CTATGTTAATTTTCCTGTTT+AGG - tig0024922:43612-43631 Msa1421290:CDS 25.0%
!!! ATTTTAAGTCACTTAAGCAA+TGG + tig0024922:43750-43769 None:intergenic 25.0%
! ATTGCTTAAGTGACTTAAAA+TGG - tig0024922:43749-43768 Msa1421290:intron 25.0%
! TAATATATTGTGCTATGACA+TGG - tig0024922:43873-43892 Msa1421290:CDS 25.0%
TCTTGTATCCTAGAATTTGA+TGG - tig0024922:42049-42068 Msa1421290:three_prime_UTR 30.0%
GATGAGTTATAATAGACGTA+AGG + tig0024922:42378-42397 None:intergenic 30.0%
TGTTAATGCATACACTGTAA+GGG + tig0024922:42433-42452 None:intergenic 30.0%
ATGTTAATGCATACACTGTA+AGG + tig0024922:42434-42453 None:intergenic 30.0%
CATCACTCATTTCATTCATA+TGG + tig0024922:42491-42510 None:intergenic 30.0%
TTGGTACCCAAAATATTACA+CGG - tig0024922:42603-42622 Msa1421290:intron 30.0%
! CAAATCACCGTGTAATATTT+TGG + tig0024922:42613-42632 None:intergenic 30.0%
TATATATCAAGCAATTAGGC+AGG + tig0024922:42703-42722 None:intergenic 30.0%
AAGTCAACAACCCAAATTTA+AGG - tig0024922:42814-42833 Msa1421290:intron 30.0%
TTTAAGGAACCTTTGTAATG+AGG - tig0024922:42830-42849 Msa1421290:intron 30.0%
! GTTTCTCTGGAAAACTTAAA+AGG - tig0024922:42988-43007 Msa1421290:intron 30.0%
CATAAACGATTCTCAAATGT+TGG + tig0024922:43038-43057 None:intergenic 30.0%
! GTTTTTCCTGTTAAAGCATA+TGG + tig0024922:43119-43138 None:intergenic 30.0%
!!! CAAGACTTTTGAGTTCTTAT+AGG - tig0024922:43304-43323 Msa1421290:intron 30.0%
!!! ATGTTTTTCTAAAGCAGCTT+TGG + tig0024922:43377-43396 None:intergenic 30.0%
! TGATGTTTGGATTTTATGCT+TGG - tig0024922:43780-43799 Msa1421290:intron 30.0%
GAAATTCAGAAACATTCACA+TGG + tig0024922:43809-43828 None:intergenic 30.0%
! TGTTCATGTCATTTCATAGA+TGG + tig0024922:42015-42034 None:intergenic 30.0%
TCTTGTATCCTAGAATTTGA+TGG - tig0024922:42049-42068 Msa1421290:three_prime_UTR 30.0%
GATGAGTTATAATAGACGTA+AGG + tig0024922:42378-42397 None:intergenic 30.0%
TGTTAATGCATACACTGTAA+GGG + tig0024922:42433-42452 None:intergenic 30.0%
ATGTTAATGCATACACTGTA+AGG + tig0024922:42434-42453 None:intergenic 30.0%
CATCACTCATTTCATTCATA+TGG + tig0024922:42491-42510 None:intergenic 30.0%
TTGGTACCCAAAATATTACA+CGG - tig0024922:42603-42622 Msa1421290:intron 30.0%
! CAAATCACCGTGTAATATTT+TGG + tig0024922:42613-42632 None:intergenic 30.0%
TATATATCAAGCAATTAGGC+AGG + tig0024922:42703-42722 None:intergenic 30.0%
AAGTCAACAACCCAAATTTA+AGG - tig0024922:42814-42833 Msa1421290:intron 30.0%
TTTAAGGAACCTTTGTAATG+AGG - tig0024922:42830-42849 Msa1421290:intron 30.0%
! GTTTCTCTGGAAAACTTAAA+AGG - tig0024922:42988-43007 Msa1421290:intron 30.0%
CATAAACGATTCTCAAATGT+TGG + tig0024922:43038-43057 None:intergenic 30.0%
! GTTTTTCCTGTTAAAGCATA+TGG + tig0024922:43119-43138 None:intergenic 30.0%
!!! CAAGACTTTTGAGTTCTTAT+AGG - tig0024922:43304-43323 Msa1421290:intron 30.0%
!!! ATGTTTTTCTAAAGCAGCTT+TGG + tig0024922:43377-43396 None:intergenic 30.0%
! TGATGTTTGGATTTTATGCT+TGG - tig0024922:43780-43799 Msa1421290:intron 30.0%
GAAATTCAGAAACATTCACA+TGG + tig0024922:43809-43828 None:intergenic 30.0%
AACCCTTTATGTCTCTTTGT+AGG - tig0024922:42201-42220 Msa1421290:three_prime_UTR 35.0%
GACCTACAAAGAGACATAAA+GGG + tig0024922:42206-42225 None:intergenic 35.0%
! TAAATTCCTTGTTTGCAAGC+AGG - tig0024922:42401-42420 Msa1421290:intron 35.0%
!! GTGTAATATTTTGGGTACCA+AGG + tig0024922:42604-42623 None:intergenic 35.0%
!!! ATTGGTTTTGCAACAGTTTG+AGG - tig0024922:42782-42801 Msa1421290:intron 35.0%
!!! TTGGTTTTGCAACAGTTTGA+GGG - tig0024922:42783-42802 Msa1421290:intron 35.0%
GGTATACACTTTGTCTCATT+TGG - tig0024922:42905-42924 Msa1421290:intron 35.0%
CTGGAAAACTTAAAAGGTTG+AGG - tig0024922:42994-43013 Msa1421290:intron 35.0%
! GAGAGGCATGCTAATTAAAA+AGG + tig0024922:43079-43098 None:intergenic 35.0%
TCACTTGTATTTGGTTGCAA+TGG - tig0024922:43400-43419 Msa1421290:intron 35.0%
GTAGTAATATTCAACTTCGC+CGG + tig0024922:43546-43565 None:intergenic 35.0%
ATGCCACTGTCAAAAACTAT+TGG - tig0024922:43586-43605 Msa1421290:CDS 35.0%
! TTGCCAATAGTTTTTGACAG+TGG + tig0024922:43592-43611 None:intergenic 35.0%
ATGGTGAGATATACACATCA+TGG + tig0024922:43731-43750 None:intergenic 35.0%
!! AATGGTGTTGCTTTGATGTT+TGG - tig0024922:43767-43786 Msa1421290:intron 35.0%
TGTCTTCATCTGTCTTCATT+TGG - tig0024922:43836-43855 Msa1421290:CDS 35.0%
AACCCTTTATGTCTCTTTGT+AGG - tig0024922:42201-42220 Msa1421290:three_prime_UTR 35.0%
GACCTACAAAGAGACATAAA+GGG + tig0024922:42206-42225 None:intergenic 35.0%
! TAAATTCCTTGTTTGCAAGC+AGG - tig0024922:42401-42420 Msa1421290:intron 35.0%
!! GTGTAATATTTTGGGTACCA+AGG + tig0024922:42604-42623 None:intergenic 35.0%
!!! ATTGGTTTTGCAACAGTTTG+AGG - tig0024922:42782-42801 Msa1421290:intron 35.0%
!!! TTGGTTTTGCAACAGTTTGA+GGG - tig0024922:42783-42802 Msa1421290:intron 35.0%
GGTATACACTTTGTCTCATT+TGG - tig0024922:42905-42924 Msa1421290:intron 35.0%
CTGGAAAACTTAAAAGGTTG+AGG - tig0024922:42994-43013 Msa1421290:intron 35.0%
! GAGAGGCATGCTAATTAAAA+AGG + tig0024922:43079-43098 None:intergenic 35.0%
TCACTTGTATTTGGTTGCAA+TGG - tig0024922:43400-43419 Msa1421290:intron 35.0%
GTAGTAATATTCAACTTCGC+CGG + tig0024922:43546-43565 None:intergenic 35.0%
ATGCCACTGTCAAAAACTAT+TGG - tig0024922:43586-43605 Msa1421290:CDS 35.0%
! TTGCCAATAGTTTTTGACAG+TGG + tig0024922:43592-43611 None:intergenic 35.0%
ATGGTGAGATATACACATCA+TGG + tig0024922:43731-43750 None:intergenic 35.0%
!! AATGGTGTTGCTTTGATGTT+TGG - tig0024922:43767-43786 Msa1421290:intron 35.0%
TGTCTTCATCTGTCTTCATT+TGG - tig0024922:43836-43855 Msa1421290:CDS 35.0%
TGGATGCTCCATCAAATTCT+AGG + tig0024922:42060-42079 None:intergenic 40.0%
GGACCTACAAAGAGACATAA+AGG + tig0024922:42207-42226 None:intergenic 40.0%
TATGTCTCTTTGTAGGTCCA+TGG - tig0024922:42208-42227 Msa1421290:three_prime_UTR 40.0%
CATGAACATGCTCATATCCT+TGG + tig0024922:42315-42334 None:intergenic 40.0%
AACTAACCTGCTTGCAAACA+AGG + tig0024922:42354-42373 None:intergenic 40.0%
TCTGCATTAACAGCAATGCA+AGG - tig0024922:42660-42679 Msa1421290:CDS 40.0%
!! ATTTGGGTTGTTGACTTTCC+AGG + tig0024922:42811-42830 None:intergenic 40.0%
TGAGACAAAGTGTATACCCT+AGG + tig0024922:42903-42922 None:intergenic 40.0%
ACCTTGATTGTGTGTTTCTC+TGG - tig0024922:42975-42994 Msa1421290:intron 40.0%
TCCAGAGAAACACACAATCA+AGG + tig0024922:42979-42998 None:intergenic 40.0%
! TAGTTTTTGACAGTGGCATG+CGG + tig0024922:43585-43604 None:intergenic 40.0%
TGCCTACTTGTTCAGCATTT+AGG - tig0024922:43638-43657 Msa1421290:CDS 40.0%
GCCTACTTGTTCAGCATTTA+GGG - tig0024922:43639-43658 Msa1421290:CDS 40.0%
CCTACTTGTTCAGCATTTAG+GGG - tig0024922:43640-43659 Msa1421290:CDS 40.0%
CTACTTGTTCAGCATTTAGG+GGG - tig0024922:43641-43660 Msa1421290:CDS 40.0%
TTAACACCATCCAGTCATGT+TGG + tig0024922:43899-43918 None:intergenic 40.0%
TGGATGCTCCATCAAATTCT+AGG + tig0024922:42060-42079 None:intergenic 40.0%
GGACCTACAAAGAGACATAA+AGG + tig0024922:42207-42226 None:intergenic 40.0%
TATGTCTCTTTGTAGGTCCA+TGG - tig0024922:42208-42227 Msa1421290:three_prime_UTR 40.0%
CATGAACATGCTCATATCCT+TGG + tig0024922:42315-42334 None:intergenic 40.0%
AACTAACCTGCTTGCAAACA+AGG + tig0024922:42354-42373 None:intergenic 40.0%
TCTGCATTAACAGCAATGCA+AGG - tig0024922:42660-42679 Msa1421290:CDS 40.0%
!! ATTTGGGTTGTTGACTTTCC+AGG + tig0024922:42811-42830 None:intergenic 40.0%
TGAGACAAAGTGTATACCCT+AGG + tig0024922:42903-42922 None:intergenic 40.0%
ACCTTGATTGTGTGTTTCTC+TGG - tig0024922:42975-42994 Msa1421290:intron 40.0%
TCCAGAGAAACACACAATCA+AGG + tig0024922:42979-42998 None:intergenic 40.0%
! TAGTTTTTGACAGTGGCATG+CGG + tig0024922:43585-43604 None:intergenic 40.0%
TGCCTACTTGTTCAGCATTT+AGG - tig0024922:43638-43657 Msa1421290:CDS 40.0%
GCCTACTTGTTCAGCATTTA+GGG - tig0024922:43639-43658 Msa1421290:CDS 40.0%
CCTACTTGTTCAGCATTTAG+GGG - tig0024922:43640-43659 Msa1421290:CDS 40.0%
CTACTTGTTCAGCATTTAGG+GGG - tig0024922:43641-43660 Msa1421290:CDS 40.0%
TTAACACCATCCAGTCATGT+TGG + tig0024922:43899-43918 None:intergenic 40.0%
! GAATTTGATGGAGCATCCAG+TGG - tig0024922:42061-42080 Msa1421290:three_prime_UTR 45.0%
!!! TTTTGGGGCTGAATGAAGCT+AGG - tig0024922:42287-42306 Msa1421290:three_prime_UTR 45.0%
CTGAATGAAGCTAGGAACCA+AGG - tig0024922:42295-42314 Msa1421290:three_prime_UTR 45.0%
TATGAGCATGTTCATGTCCG+AGG - tig0024922:42319-42338 Msa1421290:three_prime_UTR 45.0%
AAACAAGCTGAGAGTCACCT+CGG + tig0024922:42339-42358 None:intergenic 45.0%
GAACCTTTGTAATGAGGCTG+TGG - tig0024922:42836-42855 Msa1421290:intron 45.0%
! TCAGTGTCGAACATGTTCCT+AGG - tig0024922:42883-42902 Msa1421290:intron 45.0%
! CAGTGTCGAACATGTTCCTA+GGG - tig0024922:42884-42903 Msa1421290:intron 45.0%
ATCGCGTGACTTATGTAGAG+AGG + tig0024922:43096-43115 None:intergenic 45.0%
CGCGATCCATATGCTTTAAC+AGG - tig0024922:43110-43129 Msa1421290:CDS 45.0%
GCAAATCGCGGCAAAAATCT+TGG - tig0024922:43268-43287 Msa1421290:intron 45.0%
AATCGCGGCAAAAATCTTGG+AGG - tig0024922:43271-43290 Msa1421290:intron 45.0%
CAAGTAGGCACAACCTAAAC+AGG + tig0024922:43628-43647 None:intergenic 45.0%
CCCCTAAATGCTGAACAAGT+AGG + tig0024922:43643-43662 None:intergenic 45.0%
TATGACATGGCCAACATGAC+TGG - tig0024922:43886-43905 Msa1421290:CDS 45.0%
! GAATTTGATGGAGCATCCAG+TGG - tig0024922:42061-42080 Msa1421290:three_prime_UTR 45.0%
!!! TTTTGGGGCTGAATGAAGCT+AGG - tig0024922:42287-42306 Msa1421290:three_prime_UTR 45.0%
CTGAATGAAGCTAGGAACCA+AGG - tig0024922:42295-42314 Msa1421290:three_prime_UTR 45.0%
TATGAGCATGTTCATGTCCG+AGG - tig0024922:42319-42338 Msa1421290:three_prime_UTR 45.0%
AAACAAGCTGAGAGTCACCT+CGG + tig0024922:42339-42358 None:intergenic 45.0%
GAACCTTTGTAATGAGGCTG+TGG - tig0024922:42836-42855 Msa1421290:intron 45.0%
! TCAGTGTCGAACATGTTCCT+AGG - tig0024922:42883-42902 Msa1421290:intron 45.0%
! CAGTGTCGAACATGTTCCTA+GGG - tig0024922:42884-42903 Msa1421290:intron 45.0%
ATCGCGTGACTTATGTAGAG+AGG + tig0024922:43096-43115 None:intergenic 45.0%
CGCGATCCATATGCTTTAAC+AGG - tig0024922:43110-43129 Msa1421290:CDS 45.0%
GCAAATCGCGGCAAAAATCT+TGG - tig0024922:43268-43287 Msa1421290:intron 45.0%
AATCGCGGCAAAAATCTTGG+AGG - tig0024922:43271-43290 Msa1421290:intron 45.0%
CAAGTAGGCACAACCTAAAC+AGG + tig0024922:43628-43647 None:intergenic 45.0%
CCCCTAAATGCTGAACAAGT+AGG + tig0024922:43643-43662 None:intergenic 45.0%
TATGACATGGCCAACATGAC+TGG - tig0024922:43886-43905 Msa1421290:CDS 45.0%
!!! ATTTTTTTTATATTTGTATT+TGG - tig0024922:43453-43472 Msa1421290:intron 5.0%
!!! ATTTTTTTTATATTTGTATT+TGG - tig0024922:43453-43472 Msa1421290:intron 5.0%
CATCCAGTGGAAATCCTGGA+AGG - tig0024922:42074-42093 Msa1421290:three_prime_UTR 50.0%
AGACCTTCCAGGATTTCCAC+TGG + tig0024922:42080-42099 None:intergenic 50.0%
AGTGGAAATCCTGGAAGGTC+TGG - tig0024922:42079-42098 Msa1421290:three_prime_UTR 50.0%
CTGTTCTGCGTTCTGCTGAT+GGG - tig0024922:42110-42129 Msa1421290:three_prime_UTR 50.0%
!! TTGTTTGAGTGCCGAGTCCT+TGG + tig0024922:42240-42259 None:intergenic 50.0%
! CTCTCAGCTTGTTTGCAAGC+AGG - tig0024922:42345-42364 Msa1421290:three_prime_UTR 50.0%
!! GTTTTCCTATGCGTCGACCT+TGG - tig0024922:42584-42603 Msa1421290:intron 50.0%
TGCAACAGTTTGAGGGTTCC+TGG - tig0024922:42790-42809 Msa1421290:intron 50.0%
GCTCCACAGCCTCATTACAA+AGG + tig0024922:42842-42861 None:intergenic 50.0%
GCTGATGCTCAAGCAAATCG+CGG - tig0024922:43256-43275 Msa1421290:intron 50.0%
ACATGGCCAACATGACTGGA+TGG - tig0024922:43890-43909 Msa1421290:CDS 50.0%
CATCCAGTGGAAATCCTGGA+AGG - tig0024922:42074-42093 Msa1421290:three_prime_UTR 50.0%
AGACCTTCCAGGATTTCCAC+TGG + tig0024922:42080-42099 None:intergenic 50.0%
AGTGGAAATCCTGGAAGGTC+TGG - tig0024922:42079-42098 Msa1421290:three_prime_UTR 50.0%
CTGTTCTGCGTTCTGCTGAT+GGG - tig0024922:42110-42129 Msa1421290:three_prime_UTR 50.0%
!! TTGTTTGAGTGCCGAGTCCT+TGG + tig0024922:42240-42259 None:intergenic 50.0%
! CTCTCAGCTTGTTTGCAAGC+AGG - tig0024922:42345-42364 Msa1421290:three_prime_UTR 50.0%
!! GTTTTCCTATGCGTCGACCT+TGG - tig0024922:42584-42603 Msa1421290:intron 50.0%
TGCAACAGTTTGAGGGTTCC+TGG - tig0024922:42790-42809 Msa1421290:intron 50.0%
GCTCCACAGCCTCATTACAA+AGG + tig0024922:42842-42861 None:intergenic 50.0%
GCTGATGCTCAAGCAAATCG+CGG - tig0024922:43256-43275 Msa1421290:intron 50.0%
ACATGGCCAACATGACTGGA+TGG - tig0024922:43890-43909 Msa1421290:CDS 50.0%
! GGAGCATCCAGTGGAAATCC+TGG - tig0024922:42070-42089 Msa1421290:three_prime_UTR 55.0%
AATCCTGGAAGGTCTGGTGC+AGG - tig0024922:42085-42104 Msa1421290:three_prime_UTR 55.0%
GCTGTTCTGCGTTCTGCTGA+TGG - tig0024922:42109-42128 Msa1421290:three_prime_UTR 55.0%
TGCGTTCTGCTGATGGGAGT+CGG - tig0024922:42116-42135 Msa1421290:three_prime_UTR 55.0%
! GGAGCATCCAGTGGAAATCC+TGG - tig0024922:42070-42089 Msa1421290:three_prime_UTR 55.0%
AATCCTGGAAGGTCTGGTGC+AGG - tig0024922:42085-42104 Msa1421290:three_prime_UTR 55.0%
GCTGTTCTGCGTTCTGCTGA+TGG - tig0024922:42109-42128 Msa1421290:three_prime_UTR 55.0%
TGCGTTCTGCTGATGGGAGT+CGG - tig0024922:42116-42135 Msa1421290:three_prime_UTR 55.0%
GCGTTCTGCTGATGGGAGTC+GGG - tig0024922:42117-42136 Msa1421290:three_prime_UTR 60.0%
TAGGTCCATGGCGTCAGCCA+AGG - tig0024922:42220-42239 Msa1421290:three_prime_UTR 60.0%
CATGGCGTCAGCCAAGGACT+CGG - tig0024922:42226-42245 Msa1421290:three_prime_UTR 60.0%
GGGTACCAAGGTCGACGCAT+AGG + tig0024922:42592-42611 None:intergenic 60.0%
GCGTTCTGCTGATGGGAGTC+GGG - tig0024922:42117-42136 Msa1421290:three_prime_UTR 60.0%
TAGGTCCATGGCGTCAGCCA+AGG - tig0024922:42220-42239 Msa1421290:three_prime_UTR 60.0%
CATGGCGTCAGCCAAGGACT+CGG - tig0024922:42226-42245 Msa1421290:three_prime_UTR 60.0%
GGGTACCAAGGTCGACGCAT+AGG + tig0024922:42592-42611 None:intergenic 60.0%
GCTCCTGCACCAGACCTTCC+AGG + tig0024922:42091-42110 None:intergenic 65.0%
CGAGTCCTTGGCTGACGCCA+TGG + tig0024922:42228-42247 None:intergenic 65.0%
GCTCCTGCACCAGACCTTCC+AGG + tig0024922:42091-42110 None:intergenic 65.0%
CGAGTCCTTGGCTGACGCCA+TGG + tig0024922:42228-42247 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0024922 gene 42014 43928 42014 ID=Msa1421290;Name=Msa1421290
tig0024922 mRNA 42014 43928 42014 ID=Msa1421290-mRNA-1;Parent=Msa1421290;Name=Msa1421290-mRNA-1;_AED=0.00;_eAED=0.00;_QI=14|1|1|1|1|1|5|360|146
tig0024922 exon 42014 42399 42014 ID=Msa1421290-mRNA-1:exon:2025;Parent=Msa1421290-mRNA-1
tig0024922 exon 42650 42701 42650 ID=Msa1421290-mRNA-1:exon:2024;Parent=Msa1421290-mRNA-1
tig0024922 exon 43037 43144 43037 ID=Msa1421290-mRNA-1:exon:2023;Parent=Msa1421290-mRNA-1
tig0024922 exon 43576 43719 43576 ID=Msa1421290-mRNA-1:exon:2022;Parent=Msa1421290-mRNA-1
tig0024922 exon 43804 43928 43804 ID=Msa1421290-mRNA-1:exon:2021;Parent=Msa1421290-mRNA-1
tig0024922 five_prime_UTR 43915 43928 43915 ID=Msa1421290-mRNA-1:five_prime_utr;Parent=Msa1421290-mRNA-1
tig0024922 CDS 43804 43914 43804 ID=Msa1421290-mRNA-1:cds;Parent=Msa1421290-mRNA-1
tig0024922 CDS 43576 43719 43576 ID=Msa1421290-mRNA-1:cds;Parent=Msa1421290-mRNA-1
tig0024922 CDS 43037 43144 43037 ID=Msa1421290-mRNA-1:cds;Parent=Msa1421290-mRNA-1
tig0024922 CDS 42650 42701 42650 ID=Msa1421290-mRNA-1:cds;Parent=Msa1421290-mRNA-1
tig0024922 CDS 42374 42399 42374 ID=Msa1421290-mRNA-1:cds;Parent=Msa1421290-mRNA-1
tig0024922 three_prime_UTR 42014 42373 42014 ID=Msa1421290-mRNA-1:three_prime_utr;Parent=Msa1421290-mRNA-1
Gene Sequence

>Msa1421290

ATGAACAAAATCTTGCAGCGTTCTTGTATCCTAGAATTTGATGGAGCATCCAGTGGAAATCCTGGAAGGTCTGGTGCAGGAGCTGTTCTGCGTTCTGCTGATGGGAGTCGGGTCCATGGCGTCAGCCAAGGACTCGGCACTCAAACAAATAATTCTGCTGAATATAATGGTCTAATTTTGGGGCTGAATGAAGCTAGGAACCAAGGATATGAGCATGTTCATGTCCGAGGTGACTCTCAGCTTGTTTGCAAGCAGTTTGAGGGTTCCTGGAAAGTCAACAACCCAAATTTAAGGAACCTTTGTAATGAGGCTGTGGAGCTGAAGAGTAACTTCAAGTCAGTCAGTGTCGAACATGTTCCTAGGGGTTCTAACTCTGCAGCTGATGCTCAAGCAAATCGCGGCAAAAATCTTGGAGCCGGCGAAGTTGAATATTACTACTGA
Protein sequence

>Msa1421290

MNKILQRSCILEFDGASSGNPGRSGAGAVLRSADGSRVHGVSQGLGTQTNNSAEYNGLILGLNEARNQGYEHVHVRGDSQLVCKQFEGSWKVNNPNLRNLCNEAVELKSNFKSVSVEHVPRGSNSAADAQANRGKNLGAGEVEYYY*