AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0244150

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0244150 MtrunA17_Chr2g0297871 93.878 637 32 1 179 808 27 663 0.0 1214
Msa0244150 MtrunA17_Chr4g0071991 60.258 697 275 2 3 697 8 704 0.0 830
Msa0244150 MtrunA17_Chr2g0297861 97.238 181 5 0 1 181 1 181 2.32e-121 362
Msa0244150 MtrunA17_Chr2g0297051 32.253 648 362 19 85 667 343 978 2.21e-80 279
Msa0244150 MtrunA17_Chr4g0052521 27.882 746 437 19 12 664 203 940 4.06e-74 261
Msa0244150 MtrunA17_Chr8g0339181 30.795 604 349 16 128 681 347 931 8.17e-73 256
Msa0244150 MtrunA17_Chr5g0397621 30.147 544 333 10 156 664 313 844 5.70e-72 253
Msa0244150 MtrunA17_Chr4g0050431 32.796 558 328 12 128 648 310 857 1.93e-67 241
Msa0244150 MtrunA17_Chr4g0053441 29.017 641 379 14 117 693 163 791 2.84e-66 236
Msa0244150 MtrunA17_Chr3g0136331 31.992 497 295 11 204 664 355 844 1.32e-64 232
Msa0244150 MtrunA17_Chr8g0374321 28.529 340 203 7 94 399 337 670 1.40e-35 144
Msa0244150 MtrunA17_Chr8g0374371 30.952 168 98 3 201 352 22 187 1.65e-21 94.4
Msa0244150 MtrunA17_Chr8g0374391 32.338 201 117 7 509 699 35 226 1.35e-20 92.0
Msa0244150 MtrunA17_Chr8g0374331 32.338 201 117 7 509 699 42 233 1.45e-20 92.0
Msa0244150 MtrunA17_Chr2g0298031 24.587 545 336 18 214 691 160 696 2.68e-18 90.1
Msa0244150 MtrunA17_Chr3g0138931 24.044 549 333 20 214 691 155 690 1.37e-17 87.8
Msa0244150 MtrunA17_Chr6g0452721 23.377 539 349 18 214 691 157 692 1.64e-17 87.8
Msa0244150 MtrunA17_Chr1g0148031 22.769 549 340 19 214 691 155 690 7.97e-17 85.5
Msa0244150 MtrunA17_Chr1g0147981 23.748 539 330 16 214 684 172 697 8.61e-17 85.5
Msa0244150 MtrunA17_Chr4g0059961 23.162 544 344 18 214 691 159 694 1.33e-16 84.7
Msa0244150 MtrunA17_Chr8g0365021 23.400 547 339 19 214 691 225 760 1.35e-16 84.7
Msa0244150 MtrunA17_Chr2g0304941 22.686 551 338 19 214 691 156 691 1.89e-15 80.9
Msa0244150 MtrunA17_Chr8g0346461 27.309 249 136 7 509 712 619 867 1.03e-14 78.6
Msa0244150 MtrunA17_Chr2g0333721 23.913 552 330 23 214 691 153 688 1.21e-14 78.2
Msa0244150 MtrunA17_Chr8g0355421 27.143 210 115 6 506 677 636 845 3.14e-14 77.0
Msa0244150 MtrunA17_Chr7g0260401 30.303 165 83 3 506 638 669 833 3.75e-14 77.0
Msa0244150 MtrunA17_Chr7g0263361 26.484 219 120 5 509 686 626 844 4.15e-14 76.6
Msa0244150 MtrunA17_Chr6g0456201 28.571 210 109 8 508 676 640 849 2.07e-13 74.3
Msa0244150 MtrunA17_Chr3g0134951 27.711 249 134 10 508 712 582 828 5.21e-13 73.2
Msa0244150 MtrunA17_Chr4g0002331 29.814 161 82 4 509 638 709 869 1.38e-12 71.6
Msa0244150 MtrunA17_Chr2g0298871 26.702 191 106 4 482 638 666 856 4.91e-12 70.1
Msa0244150 MtrunA17_Chr8g0382071 27.168 173 99 3 493 638 632 804 5.53e-12 69.7
Msa0244150 MtrunA17_Chr3g0134771 26.923 208 115 7 506 676 648 855 2.11e-11 67.8
Msa0244150 MtrunA17_Chr4g0020551 28.662 157 86 3 508 638 583 739 4.57e-11 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0244150 AT4G30110.1 56.143 757 311 6 3 748 4 750 0.0 859
Msa0244150 AT4G30110.2 56.143 757 311 6 3 748 4 750 0.0 859
Msa0244150 AT2G19110.2 56.250 784 308 8 5 765 16 787 0.0 857
Msa0244150 AT2G19110.3 56.250 784 308 8 5 765 16 787 0.0 857
Msa0244150 AT2G19110.1 56.250 784 308 8 5 765 16 787 0.0 857
Msa0244150 AT4G30120.1 55.970 536 225 3 4 532 11 542 0.0 613
Msa0244150 AT4G30120.2 58.398 387 150 3 153 532 1 383 2.31e-160 471
Msa0244150 AT5G44790.1 29.216 753 419 17 10 662 211 949 6.48e-79 275
Msa0244150 AT1G63440.1 30.354 537 324 11 156 654 406 930 4.54e-70 249
Msa0244150 AT4G33520.2 31.012 603 349 12 156 704 348 937 2.33e-66 238
Msa0244150 AT4G33520.3 31.156 597 356 13 156 704 348 937 3.11e-66 238
Msa0244150 AT5G21930.2 28.918 619 371 16 110 662 221 836 1.06e-60 221
Msa0244150 AT5G21930.1 28.918 619 371 16 110 662 221 836 1.06e-60 221
Msa0244150 AT5G21930.4 29.302 587 346 16 110 630 221 804 4.99e-57 210
Msa0244150 AT4G37270.1 29.206 630 377 18 119 688 161 781 5.65e-54 201
Msa0244150 AT5G21930.3 28.595 619 350 17 110 662 221 813 1.96e-53 200
Msa0244150 AT5G57350.4 23.626 546 339 19 214 691 154 689 8.24e-18 89.0
Msa0244150 AT5G57350.2 23.626 546 339 19 214 691 154 689 8.24e-18 89.0
Msa0244150 AT5G57350.1 23.626 546 339 19 214 691 154 689 8.24e-18 89.0
Msa0244150 AT3G60330.4 23.370 552 344 17 204 683 146 690 2.74e-17 87.4
Msa0244150 AT3G60330.3 23.370 552 344 17 204 683 146 690 2.74e-17 87.4
Msa0244150 AT3G60330.2 23.370 552 344 17 204 683 146 690 2.96e-17 87.4
Msa0244150 AT3G60330.1 23.370 552 344 17 204 683 146 690 2.96e-17 87.4
Msa0244150 AT3G47950.1 23.260 546 341 17 214 691 161 696 7.58e-17 85.9
Msa0244150 AT3G47950.2 23.260 546 341 17 214 691 161 696 7.58e-17 85.9
Msa0244150 AT2G24520.3 22.486 547 344 18 214 691 135 670 2.08e-16 84.3
Msa0244150 AT2G24520.1 22.486 547 344 18 214 691 135 670 2.08e-16 84.3
Msa0244150 AT2G24520.2 22.486 547 344 18 214 691 198 733 2.12e-16 84.7
Msa0244150 AT1G80660.1 23.358 548 338 22 214 691 158 693 4.81e-16 83.2
Msa0244150 AT1G80660.3 23.358 548 338 22 214 691 158 693 4.98e-16 83.2
Msa0244150 AT1G80660.2 23.358 548 338 22 214 691 29 564 5.12e-16 83.2
Msa0244150 AT1G80660.4 23.358 548 338 22 214 691 158 693 5.16e-16 83.2
Msa0244150 AT1G17260.1 23.116 584 337 23 214 712 161 717 8.21e-16 82.4
Msa0244150 AT2G18960.1 23.260 546 341 18 214 691 153 688 9.93e-16 82.4
Msa0244150 AT2G18960.3 23.292 571 343 22 214 707 89 641 1.66e-15 81.6
Msa0244150 AT2G18960.2 23.292 571 343 22 214 707 89 641 1.66e-15 81.6
Msa0244150 AT5G62670.1 22.744 554 334 19 214 691 157 692 2.71e-15 80.9
Msa0244150 AT2G07560.1 23.146 553 332 19 214 691 156 690 4.04e-14 77.0
Msa0244150 AT1G07810.1 27.854 219 117 6 509 686 621 839 4.04e-14 77.0
Msa0244150 AT2G41560.2 31.447 159 83 4 506 638 538 696 4.82e-14 77.0
Msa0244150 AT2G41560.3 31.447 159 83 4 506 638 538 696 4.82e-14 77.0
Msa0244150 AT2G41560.4 31.447 159 83 4 506 638 538 696 4.82e-14 77.0
Msa0244150 AT2G41560.1 31.447 159 83 4 506 638 640 798 6.01e-14 76.6
Msa0244150 AT3G42640.1 21.681 678 398 22 99 691 62 691 7.60e-14 76.3
Msa0244150 AT3G21180.1 31.056 161 80 3 509 638 691 851 8.15e-14 76.3
Msa0244150 AT1G10130.1 24.427 262 154 8 494 712 567 827 8.41e-14 76.3
Msa0244150 AT3G57330.2 31.056 161 85 3 504 638 580 740 1.35e-13 75.5
Msa0244150 AT1G07670.2 27.397 219 118 6 509 686 621 839 1.53e-13 75.5
Msa0244150 AT1G07670.1 27.397 219 118 6 509 686 621 839 1.53e-13 75.5
Msa0244150 AT3G57330.1 31.056 161 85 3 504 638 635 795 1.55e-13 75.1
Msa0244150 AT4G00900.2 28.436 211 108 6 509 676 615 825 2.20e-13 74.7
Msa0244150 AT4G00900.1 28.436 211 108 6 509 676 615 825 2.20e-13 74.7
Msa0244150 AT5G57110.1 27.950 161 85 3 509 638 674 834 1.25e-12 72.4
Msa0244150 AT5G57110.3 27.950 161 85 3 509 638 674 834 1.25e-12 72.4
Msa0244150 AT5G57110.2 27.950 161 85 3 509 638 674 834 1.25e-12 72.4

Find 135 sgRNAs with CRISPR-Local

Find 256 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTCCCTAAAGTTCTATTT+AGG 0.130187 2_2:+22334742 Msa0244150:CDS
TACTCTTAATATCAACATTT+TGG 0.177691 2_2:+22334788 Msa0244150:CDS
GGTAATATTGATGAGGAAAA+AGG 0.221060 2_2:+22338271 Msa0244150:CDS
AAAGCCCTAAATAGAACTTT+AGG 0.262334 2_2:-22334747 None:intergenic
GACAAGTGAATCTGATTCTT+TGG 0.271282 2_2:-22335600 None:intergenic
TCAACCAAAGGTTGGAAATA+TGG 0.276728 2_2:+22338205 Msa0244150:CDS
CTTATTTCTGAGTCACAAAT+AGG 0.288449 2_2:+22334265 Msa0244150:CDS
ATTCCCTAAAGTTCTATTTA+GGG 0.294365 2_2:+22334743 Msa0244150:CDS
CATGCCATTGTAAGTATATC+TGG 0.312080 2_2:-22334639 None:intergenic
GAAGTATATATATCCTCCTT+TGG 0.321866 2_2:+22334689 Msa0244150:CDS
TGCAATTGCCAATGCAAGTA+TGG 0.322173 2_2:-22338071 None:intergenic
CTTGCTAAACGAATCGCTTC+AGG 0.323239 2_2:-22337992 None:intergenic
TTCAACAAGTCTTGACATTC+GGG 0.326874 2_2:-22335985 None:intergenic
CTTCAACAAGTCTTGACATT+CGG 0.332150 2_2:-22335986 None:intergenic
TTGTAGATCAGGTGCTTTAG+AGG 0.332991 2_2:+22337578 Msa0244150:CDS
TTGCAGGTTATCCATTAGTT+TGG 0.335285 2_2:+22338089 Msa0244150:CDS
AGATTCTAGCATTGCTACTT+TGG 0.341996 2_2:+22338477 Msa0244150:CDS
CCTTTGGGATGGTTGGCTCT+TGG 0.343324 2_2:+22334705 Msa0244150:CDS
TGAAACCATGGCTCCATATC+AGG 0.347039 2_2:-22336674 None:intergenic
GATGGTAATGATATCTATAT+AGG 0.347900 2_2:+22337281 Msa0244150:CDS
TTGCTTGTTAAGTAATGGTT+TGG 0.349064 2_2:-22338248 None:intergenic
TTTGCAAAGTACTATATTCC+TGG 0.353905 2_2:+22336053 Msa0244150:CDS
TGCATTAAGTGTTCCTGATA+TGG 0.359374 2_2:+22336661 Msa0244150:CDS
AAGAATCAGATTCACTTGTC+TGG 0.360703 2_2:+22335603 Msa0244150:CDS
TTTACCATATTTCCAACCTT+TGG 0.363775 2_2:-22338209 None:intergenic
GGCATATCAATGGGAATATC+TGG 0.363797 2_2:+22337908 Msa0244150:CDS
AAGTATATATATCCTCCTTT+GGG 0.370351 2_2:+22334690 Msa0244150:CDS
ATTAAGAGTAAGAGCTCTAA+TGG 0.372369 2_2:-22334775 None:intergenic
AAGCCCTAAATAGAACTTTA+GGG 0.378170 2_2:-22334746 None:intergenic
TGAGAACTCTCCTCTTGTTA+TGG 0.378498 2_2:-22336870 None:intergenic
CTTGTTGATGCTTGTAGATC+AGG 0.378715 2_2:+22337567 Msa0244150:CDS
CCATCTCCTATCATGGCTAT+TGG 0.379600 2_2:-22337848 None:intergenic
ATACTTGCATTGGCAATTGC+AGG 0.384606 2_2:+22338073 Msa0244150:CDS
GCTCTTGGTTCTGTTGCCAT+TGG 0.386825 2_2:+22334720 Msa0244150:CDS
CTATTTGTGACTCAGAAATA+AGG 0.391338 2_2:-22334264 None:intergenic
GGTGATGCTATTCCTCTTGA+TGG 0.398393 2_2:+22335512 Msa0244150:CDS
GAGAAGCAACTTTGAAGTGT+TGG 0.403762 2_2:+22334123 Msa0244150:CDS
ATTGAGAATTTCAAGAAAGA+AGG 0.407323 2_2:+22337824 Msa0244150:CDS
ATGGAAGAGCTGAAGTCATT+AGG 0.414538 2_2:+22337603 Msa0244150:CDS
AGAAGCAACTTTGAAGTGTT+GGG 0.415170 2_2:+22334124 Msa0244150:CDS
AGAATTCTGAAGCCTCTTCA+TGG 0.416439 2_2:+22334181 Msa0244150:CDS
ATATCCTCCTTTGGGATGGT+TGG 0.437705 2_2:+22334698 Msa0244150:CDS
AACAAACATGGCTCCTCAAA+TGG 0.438346 2_2:+22335415 Msa0244150:CDS
TAATTCTAGACAGTGTTTCA+AGG 0.450623 2_2:-22336820 None:intergenic
AGCAGACATCGGCATATCAA+TGG 0.451802 2_2:+22337898 Msa0244150:CDS
TTACCTTGCTTGTTAAGTAA+TGG 0.454151 2_2:-22338253 None:intergenic
CTCACAAAAGCTGCAATAAG+TGG 0.455717 2_2:+22336773 Msa0244150:CDS
ATGGCTATTGTTGCTGAGAC+CGG 0.458297 2_2:+22335434 Msa0244150:CDS
TGCCATGTATGTTCAGAGTC+AGG 0.462679 2_2:+22337665 Msa0244150:CDS
TCCACTAGTGCACCTGCCAC+TGG 0.469705 2_2:-22337170 None:intergenic
GAATCTGATTCTTTGGTGAC+CGG 0.472736 2_2:-22335593 None:intergenic
AAAACAGCTAGCCAAACTAA+TGG 0.474489 2_2:-22338100 None:intergenic
TCAGATTCACTTGTCTGGGC+TGG 0.474967 2_2:+22335608 Msa0244150:CDS
GAAGGGCCAATAGCCATGAT+AGG 0.478048 2_2:+22337842 Msa0244150:CDS
CGAGCTTCTACCACATGAAA+AGG 0.481888 2_2:+22337793 Msa0244150:CDS
GAGAATGTCATTATTTCAGT+TGG 0.482612 2_2:+22338037 Msa0244150:CDS
TGGCTATTGTTGCTGAGACC+GGG 0.484594 2_2:+22335435 Msa0244150:CDS
GTCAGATCAGTCATGCTGAC+AGG 0.495318 2_2:+22337627 Msa0244150:CDS
CAAATGTGCCATACTTGCAT+TGG 0.496220 2_2:+22338063 Msa0244150:CDS
ATCAGGTGCTTTAGAGGCAA+TGG 0.498112 2_2:+22337584 Msa0244150:CDS
GTTTCTTGGTAGAAATCTCA+TGG 0.500125 2_2:-22337509 None:intergenic
TGAGACAAGAGCAACTCATA+AGG 0.500504 2_2:+22335022 Msa0244150:CDS
GTTAATGCCATCTCCTATCA+TGG 0.502461 2_2:-22337855 None:intergenic
TCAAAGTTGAGTTTACTCAA+TGG 0.506061 2_2:+22338391 Msa0244150:CDS
AGAATCAGATTCACTTGTCT+GGG 0.506902 2_2:+22335604 Msa0244150:CDS
ACAATGAAACCTTACTGTAC+TGG 0.509660 2_2:+22336924 Msa0244150:CDS
TCAGGAACACTTAATGCAGC+TGG 0.510078 2_2:-22336656 None:intergenic
ACCATCCTTGCAGTCAAGGC+TGG 0.510481 2_2:+22335491 Msa0244150:CDS
TCCTCTTGATGGAATTGTTG+TGG 0.512231 2_2:+22335523 Msa0244150:CDS
CAACAAACATAAACTTTCCA+TGG 0.513388 2_2:-22338420 None:intergenic
ATTGACATCAACACGTTCCC+CGG 0.514951 2_2:-22335453 None:intergenic
GCAGACATCGGCATATCAAT+GGG 0.515459 2_2:+22337899 Msa0244150:CDS
GTTAACAGGAGAATCATTCC+CGG 0.517346 2_2:+22335574 Msa0244150:CDS
TTGAGAATTTCAAGAAAGAA+GGG 0.523815 2_2:+22337825 Msa0244150:CDS
ACCAGCCTTGACTGCAAGGA+TGG 0.526545 2_2:-22335492 None:intergenic
TATATAGGAAACAAGAGAGT+TGG 0.528816 2_2:+22337296 Msa0244150:CDS
TCATTATTGGTCTCTCCTTG+TGG 0.530580 2_2:-22334606 None:intergenic
GTCAGAGCTATATGTAAAAG+AGG 0.530799 2_2:+22337320 Msa0244150:CDS
TCCAGTGGCAGGTGCACTAG+TGG 0.531604 2_2:+22337169 Msa0244150:CDS
AAAGAGAATCAACCAAAGGT+TGG 0.535439 2_2:+22338197 Msa0244150:CDS
ATGTCTGCTGTAGCTAATGC+AGG 0.535705 2_2:-22337884 None:intergenic
CAGAAATAAGGAGAACATCA+TGG 0.536453 2_2:-22334252 None:intergenic
ATGGTTTCATCTAGCACTTG+TGG 0.538419 2_2:+22336688 Msa0244150:CDS
GCTGGAACTATTAATATGAA+TGG 0.542182 2_2:+22335626 Msa0244150:CDS
GAACACCATCCTTGCAGTCA+AGG 0.543189 2_2:+22335487 Msa0244150:CDS
AGAGCACAAAAGATTGCAAC+TGG 0.543642 2_2:-22336752 None:intergenic
AATTGTGGAGAAACTCTAGT+TGG 0.544389 2_2:+22337534 Msa0244150:CDS
AAATCAAGTCATCCAGTGGC+AGG 0.547267 2_2:+22337158 Msa0244150:CDS
TTGTGCAACAGAAGCATCAC+TGG 0.556014 2_2:+22334153 Msa0244150:CDS
CATCACCAGCCTTGACTGCA+AGG 0.558354 2_2:-22335496 None:intergenic
GATTTCAGCTAGCATTGCAG+TGG 0.558685 2_2:+22336631 Msa0244150:CDS
CAAACCAGTACCAGAAAATG+TGG 0.561124 2_2:+22337214 Msa0244150:CDS
GGCTATTGTTGCTGAGACCG+GGG 0.564489 2_2:+22335436 Msa0244150:CDS
AATCTGATTCTTTGGTGACC+GGG 0.566418 2_2:-22335592 None:intergenic
ACAAGAAAAGATGAATCTTG+TGG 0.567250 2_2:+22338364 Msa0244150:CDS
TTCTGTTCTCTATTGCTCAG+TGG 0.567993 2_2:+22334997 Msa0244150:CDS
ATAAGTGGATTACTATTGAA+AGG 0.569583 2_2:+22336788 Msa0244150:CDS
CCAATAGCCATGATAGGAGA+TGG 0.570907 2_2:+22337848 Msa0244150:CDS
TCCACAACAATTCCATCAAG+AGG 0.572437 2_2:-22335524 None:intergenic
ATATATATCCTCCTTTGGGA+TGG 0.576773 2_2:+22334694 Msa0244150:CDS
GTGTTCCTGATATGGAGCCA+TGG 0.579497 2_2:+22336669 Msa0244150:CDS
TCAGCAACAATAGCCATTTG+AGG 0.581659 2_2:-22335428 None:intergenic
TACCTGACTCTGAACATACA+TGG 0.589939 2_2:-22337667 None:intergenic
GAAAACCTACCAGTACAGTA+AGG 0.591584 2_2:-22336933 None:intergenic
TCTACCAAGAAACAAAATTG+TGG 0.594583 2_2:+22337519 Msa0244150:CDS
ACAGCTTTGACTCCATGAAG+AGG 0.594671 2_2:-22334193 None:intergenic
CTTGATGGAATTGTTGTGGA+AGG 0.598010 2_2:+22335527 Msa0244150:CDS
CTGATCTACAAGCATCAACA+AGG 0.601748 2_2:-22337566 None:intergenic
CAAGCAAGGTAATATTGATG+AGG 0.619230 2_2:+22338264 Msa0244150:CDS
AAAACAGGCACCATAACAAG+AGG 0.622472 2_2:+22336860 Msa0244150:CDS
AAGTGTAAGAACAACTGTGT+TGG 0.622963 2_2:+22335944 Msa0244150:CDS
AGTGGATTACTATTGAAAGG+TGG 0.625372 2_2:+22336791 Msa0244150:CDS
GTAGAGAGGATAAGAGCACA+AGG 0.625381 2_2:-22336728 None:intergenic
AAACCATTACTTAACAAGCA+AGG 0.626884 2_2:+22338250 Msa0244150:CDS
CCAAGAGCCAACCATCCCAA+AGG 0.627193 2_2:-22334705 None:intergenic
TTACTCAATGGAAATGACCA+TGG 0.628584 2_2:+22338403 Msa0244150:CDS
GTGCACTAGTGGATTATGCA+AGG 0.630355 2_2:+22337180 Msa0244150:CDS
ATGTCCAGATATACTTACAA+TGG 0.632108 2_2:+22334635 Msa0244150:CDS
ACAACAGTAACTGTTCGAGC+AGG 0.633676 2_2:-22334229 None:intergenic
TAGAACTTTAGGGAATCCAA+TGG 0.634958 2_2:-22334736 None:intergenic
GAAGAAAGAGAATCAACCAA+AGG 0.636925 2_2:+22338193 Msa0244150:CDS
GCATTAGCTACAGCAGACAT+CGG 0.650450 2_2:+22337887 Msa0244150:CDS
AGAGCCAACCATCCCAAAGG+AGG 0.660926 2_2:-22334702 None:intergenic
AGATTGCAACTGGTGTAGAG+AGG 0.664129 2_2:-22336742 None:intergenic
AATGTCGTCTTTAACAAACA+TGG 0.667117 2_2:+22335403 Msa0244150:CDS
AAGTAAGCATGTTCCAACAT+CGG 0.668314 2_2:-22338125 None:intergenic
CGGATGCACTGAATACAGCA+AGG 0.674306 2_2:+22334565 Msa0244150:CDS
ACAAGTGCTAGATGAAACCA+TGG 0.685244 2_2:-22336686 None:intergenic
GATATACTTACAATGGCATG+CGG 0.693837 2_2:+22334642 Msa0244150:CDS
GAGTAAATCAAGTCATCCAG+TGG 0.696182 2_2:+22337154 Msa0244150:CDS
CTTCTTAGTAGTGAAGAGTG+TGG 0.712204 2_2:+22338295 Msa0244150:CDS
GAATCTTGTATCATAACACA+AGG 0.715496 2_2:-22338460 None:intergenic
TAGAGAGGATAAGAGCACAA+GGG 0.729183 2_2:-22336727 None:intergenic
ACTGTCTAGAATTAAGACAG+TGG 0.732446 2_2:+22336829 Msa0244150:CDS
TGTGTTGGCGAAAGACACAA+TGG 0.738132 2_2:+22335959 Msa0244150:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAACTATAAGAAAATAT+TGG + chr2_2:22335321-22335340 Msa0244150:intron 10.0%
!!! GATACAAATTTTTTCTTAAA+AGG - chr2_2:22336464-22336483 None:intergenic 15.0%
!! AACATGAATTTGTATATCTT+AGG + chr2_2:22334325-22334344 Msa0244150:intron 20.0%
!! ACATGAATTTGTATATCTTA+GGG + chr2_2:22334326-22334345 Msa0244150:intron 20.0%
!!! TCATAATTTACTTTGTTTCT+TGG + chr2_2:22334405-22334424 Msa0244150:intron 20.0%
!!! TACTCTTAATATCAACATTT+TGG + chr2_2:22334788-22334807 Msa0244150:CDS 20.0%
!!! TATCAACATTTTGGTTTTAT+TGG + chr2_2:22334797-22334816 Msa0244150:CDS 20.0%
!! ATGAATGTAATAATGTAACA+AGG - chr2_2:22335370-22335389 None:intergenic 20.0%
!! TGTTACATTATTACATTCAT+AGG + chr2_2:22335370-22335389 Msa0244150:intron 20.0%
!!! GATAATTGTGAGAAAAATTT+TGG - chr2_2:22335741-22335760 None:intergenic 20.0%
!! TAAATAATGAGTCTGATTAA+TGG + chr2_2:22336540-22336559 Msa0244150:intron 20.0%
!! AAATAATGAGTCTGATTAAT+GGG + chr2_2:22336541-22336560 Msa0244150:intron 20.0%
!! AATAAGCTGAAAATAGTTTA+TGG + chr2_2:22336965-22336984 Msa0244150:intron 20.0%
!! AATTTGGTTAATGAAAACTT+TGG + chr2_2:22337113-22337132 Msa0244150:intron 20.0%
!!! AGAATTTTCAAAATTTTCCA+GGG + chr2_2:22337237-22337256 Msa0244150:CDS 20.0%
!!! TTGGAAATATGGTAAATTTT+TGG + chr2_2:22338216-22338235 Msa0244150:CDS 20.0%
! TATTTATGTGATTGTTTCAG+CGG + chr2_2:22334545-22334564 Msa0244150:intron 25.0%
!! TGGACATTTCTTTTCATTAT+TGG - chr2_2:22334622-22334641 None:intergenic 25.0%
! AAGTATATATATCCTCCTTT+GGG + chr2_2:22334690-22334709 Msa0244150:CDS 25.0%
! ATTCCCTAAAGTTCTATTTA+GGG + chr2_2:22334743-22334762 Msa0244150:CDS 25.0%
! TAAAGAAAACATGTTTGCAT+TGG + chr2_2:22334871-22334890 Msa0244150:intron 25.0%
! TACATTAACGACATAAAGTT+TGG - chr2_2:22335128-22335147 None:intergenic 25.0%
! AGTACATTAATATAGCAAGA+AGG - chr2_2:22335156-22335175 None:intergenic 25.0%
! ACATTAATATGCACACTAAA+AGG - chr2_2:22335242-22335261 None:intergenic 25.0%
! ATTATTACATTCATAGGCAA+TGG + chr2_2:22335376-22335395 Msa0244150:intron 25.0%
!! GTTGATGAAAAAATGTTAAC+AGG + chr2_2:22335560-22335579 Msa0244150:CDS 25.0%
!! GTTAAGTATCTTAGAACTTT+TGG + chr2_2:22335648-22335667 Msa0244150:intron 25.0%
! GATGAAATATAAGGATAACT+AGG - chr2_2:22335855-22335874 None:intergenic 25.0%
! TGTGATAGAGATGAAATATA+AGG - chr2_2:22335864-22335883 None:intergenic 25.0%
!!! TTTTGAGTACATATCATTTC+AGG + chr2_2:22335915-22335934 Msa0244150:intron 25.0%
! TATCCTTGGATTAATAAACA+TGG + chr2_2:22336099-22336118 Msa0244150:intron 25.0%
! TGATTACCAAAAACAAATCA+TGG - chr2_2:22336238-22336257 None:intergenic 25.0%
! TAGAAAAAATGTGATTGGAA+GGG - chr2_2:22336345-22336364 None:intergenic 25.0%
! ATAGAAAAAATGTGATTGGA+AGG - chr2_2:22336346-22336365 None:intergenic 25.0%
!! GTTGATAGAAAAAATGTGAT+TGG - chr2_2:22336350-22336369 None:intergenic 25.0%
! ATGTTTATATTGTATTGCAG+TGG + chr2_2:22336601-22336620 Msa0244150:intron 25.0%
!!! ATAAGTGGATTACTATTGAA+AGG + chr2_2:22336788-22336807 Msa0244150:CDS 25.0%
!! CTTTGAAGACTAACTTAATT+TGG + chr2_2:22337097-22337116 Msa0244150:intron 25.0%
!! GAGAATTTTCAAAATTTTCC+AGG + chr2_2:22337236-22337255 Msa0244150:CDS 25.0%
!! GAATTTTCAAAATTTTCCAG+GGG + chr2_2:22337238-22337257 Msa0244150:CDS 25.0%
!! GATGGTAATGATATCTATAT+AGG + chr2_2:22337281-22337300 Msa0244150:CDS 25.0%
! ATTCACAACTTAATTCTTGA+AGG + chr2_2:22337350-22337369 Msa0244150:intron 25.0%
! TATAGCATTGTTTAACTGAA+AGG - chr2_2:22337759-22337778 None:intergenic 25.0%
! ATTGAGAATTTCAAGAAAGA+AGG + chr2_2:22337824-22337843 Msa0244150:CDS 25.0%
! TTGAGAATTTCAAGAAAGAA+GGG + chr2_2:22337825-22337844 Msa0244150:CDS 25.0%
! GTAATATTGATGAGGAAAAA+GGG + chr2_2:22338272-22338291 Msa0244150:CDS 25.0%
! TAAAAATGTTACTCATCATG+TGG + chr2_2:22338330-22338349 Msa0244150:CDS 25.0%
CTATTTGTGACTCAGAAATA+AGG - chr2_2:22334267-22334286 None:intergenic 30.0%
!! CTTATTTCTGAGTCACAAAT+AGG + chr2_2:22334265-22334284 Msa0244150:CDS 30.0%
ATGTCCAGATATACTTACAA+TGG + chr2_2:22334635-22334654 Msa0244150:CDS 30.0%
GAAGTATATATATCCTCCTT+TGG + chr2_2:22334689-22334708 Msa0244150:CDS 30.0%
GATTCCCTAAAGTTCTATTT+AGG + chr2_2:22334742-22334761 Msa0244150:CDS 30.0%
AAGCCCTAAATAGAACTTTA+GGG - chr2_2:22334749-22334768 None:intergenic 30.0%
AAAGCCCTAAATAGAACTTT+AGG - chr2_2:22334750-22334769 None:intergenic 30.0%
! ATTAAGAGTAAGAGCTCTAA+TGG - chr2_2:22334778-22334797 None:intergenic 30.0%
!!! AACATTTTGGTTTTATTGGC+AGG + chr2_2:22334801-22334820 Msa0244150:CDS 30.0%
TTTCAGACGTTTGTAGAAAA+TGG - chr2_2:22334829-22334848 None:intergenic 30.0%
! GCTTTACAAGATTTTACAGA+TGG + chr2_2:22334960-22334979 Msa0244150:CDS 30.0%
! TTACAAGATTTTACAGATGG+TGG + chr2_2:22334963-22334982 Msa0244150:CDS 30.0%
TGGTATTAATCGAGTAATGA+AGG - chr2_2:22335108-22335127 None:intergenic 30.0%
AATATGCACACTAAAAGGTT+TGG - chr2_2:22335237-22335256 None:intergenic 30.0%
AATGTCGTCTTTAACAAACA+TGG + chr2_2:22335403-22335422 Msa0244150:CDS 30.0%
GCTGGAACTATTAATATGAA+TGG + chr2_2:22335626-22335645 Msa0244150:CDS 30.0%
! AACCCAGAATCAACATTTTA+AGG + chr2_2:22335775-22335794 Msa0244150:intron 30.0%
!! CACCTTAAAATGTTGATTCT+GGG - chr2_2:22335780-22335799 None:intergenic 30.0%
!! ACACCTTAAAATGTTGATTC+TGG - chr2_2:22335781-22335800 None:intergenic 30.0%
! TTTGCAAAGTACTATATTCC+TGG + chr2_2:22336053-22336072 Msa0244150:CDS 30.0%
!!! TCAGAACCATGATTTGTTTT+TGG + chr2_2:22336229-22336248 Msa0244150:intron 30.0%
AGAAAAAATGTGATTGGAAG+GGG - chr2_2:22336344-22336363 None:intergenic 30.0%
TTCTGATACCAACATTACAT+TGG - chr2_2:22336434-22336453 None:intergenic 30.0%
TAATTCTAGACAGTGTTTCA+AGG - chr2_2:22336823-22336842 None:intergenic 30.0%
! TACTGGTAGGTTTTCATTTA+CGG + chr2_2:22336941-22336960 Msa0244150:intron 30.0%
! TAAACACTTTAGAGAGTTCA+AGG - chr2_2:22337009-22337028 None:intergenic 30.0%
TGGTTAATGAAAACTTTGGT+AGG + chr2_2:22337117-22337136 Msa0244150:intron 30.0%
! TGAAAATTCTCCACATTTTC+TGG - chr2_2:22337227-22337246 None:intergenic 30.0%
!!! GGAATTTTTGGTACAATTGA+TGG + chr2_2:22337263-22337282 Msa0244150:CDS 30.0%
TATATAGGAAACAAGAGAGT+TGG + chr2_2:22337296-22337315 Msa0244150:CDS 30.0%
TCTACCAAGAAACAAAATTG+TGG + chr2_2:22337519-22337538 Msa0244150:CDS 30.0%
!! TTCTCCACAATTTTGTTTCT+TGG - chr2_2:22337526-22337545 None:intergenic 30.0%
GAGAATGTCATTATTTCAGT+TGG + chr2_2:22338037-22338056 Msa0244150:CDS 30.0%
TTTACCATATTTCCAACCTT+TGG - chr2_2:22338212-22338231 None:intergenic 30.0%
! TTGCTTGTTAAGTAATGGTT+TGG - chr2_2:22338251-22338270 None:intergenic 30.0%
AAACCATTACTTAACAAGCA+AGG + chr2_2:22338250-22338269 Msa0244150:CDS 30.0%
! TTACCTTGCTTGTTAAGTAA+TGG - chr2_2:22338256-22338275 None:intergenic 30.0%
GGTAATATTGATGAGGAAAA+AGG + chr2_2:22338271-22338290 Msa0244150:CDS 30.0%
ACAAGAAAAGATGAATCTTG+TGG + chr2_2:22338364-22338383 Msa0244150:CDS 30.0%
TCAAAGTTGAGTTTACTCAA+TGG + chr2_2:22338391-22338410 Msa0244150:CDS 30.0%
CAACAAACATAAACTTTCCA+TGG - chr2_2:22338423-22338442 None:intergenic 30.0%
GAATCTTGTATCATAACACA+AGG - chr2_2:22338463-22338482 None:intergenic 30.0%
!! AGAAGCAACTTTGAAGTGTT+GGG + chr2_2:22334124-22334143 Msa0244150:CDS 35.0%
CAGAAATAAGGAGAACATCA+TGG - chr2_2:22334255-22334274 None:intergenic 35.0%
!!! GTTGACTTTTTTCTGTGTGA+TGG + chr2_2:22334381-22334400 Msa0244150:intron 35.0%
! TTGTTTCTTGGATTCATGTG+TGG + chr2_2:22334417-22334436 Msa0244150:intron 35.0%
CATGCCATTGTAAGTATATC+TGG - chr2_2:22334642-22334661 None:intergenic 35.0%
GATATACTTACAATGGCATG+CGG + chr2_2:22334642-22334661 Msa0244150:CDS 35.0%
! ATATATATCCTCCTTTGGGA+TGG + chr2_2:22334694-22334713 Msa0244150:CDS 35.0%
! TAGAACTTTAGGGAATCCAA+TGG - chr2_2:22334739-22334758 None:intergenic 35.0%
AGAAAACATGTTTGCATTGG+TGG + chr2_2:22334874-22334893 Msa0244150:intron 35.0%
TCATCATATGTTGCAGTATG+TGG + chr2_2:22334930-22334949 Msa0244150:intron 35.0%
TACTCTTGCTTCTTGCTTAA+GGG + chr2_2:22335198-22335217 Msa0244150:intron 35.0%
GTGTGCATATTAATGTACTC+CGG + chr2_2:22335246-22335265 Msa0244150:intron 35.0%
GACAAGTGAATCTGATTCTT+TGG - chr2_2:22335603-22335622 None:intergenic 35.0%
AAGAATCAGATTCACTTGTC+TGG + chr2_2:22335603-22335622 Msa0244150:CDS 35.0%
AGAATCAGATTCACTTGTCT+GGG + chr2_2:22335604-22335623 Msa0244150:CDS 35.0%
AAGTGTAAGAACAACTGTGT+TGG + chr2_2:22335944-22335963 Msa0244150:CDS 35.0%
TTCAACAAGTCTTGACATTC+GGG - chr2_2:22335988-22336007 None:intergenic 35.0%
CTTCAACAAGTCTTGACATT+CGG - chr2_2:22335989-22336008 None:intergenic 35.0%
AATCCAAGGATAAACATCCA+TGG - chr2_2:22336091-22336110 None:intergenic 35.0%
CCACCATGTTTATTAATCCA+AGG - chr2_2:22336105-22336124 None:intergenic 35.0%
CCTTGGATTAATAAACATGG+TGG + chr2_2:22336102-22336121 Msa0244150:intron 35.0%
TGAAAAGTCCACATTACATC+CGG + chr2_2:22336150-22336169 Msa0244150:intron 35.0%
!! AAATCATGGTTCTGAATCGA+GGG - chr2_2:22336224-22336243 None:intergenic 35.0%
TTATTCACCAACTTTGCCAA+TGG + chr2_2:22336272-22336291 Msa0244150:intron 35.0%
AGTTCATTCACATTGTGTCA+GGG + chr2_2:22336385-22336404 Msa0244150:intron 35.0%
!! CAACATTACATTGGTTCAAG+TGG - chr2_2:22336425-22336444 None:intergenic 35.0%
CACTTGAACCAATGTAATGT+TGG + chr2_2:22336423-22336442 Msa0244150:intron 35.0%
TGCATTAAGTGTTCCTGATA+TGG + chr2_2:22336661-22336680 Msa0244150:CDS 35.0%
!! AGTGGATTACTATTGAAAGG+TGG + chr2_2:22336791-22336810 Msa0244150:CDS 35.0%
! ACTGTCTAGAATTAAGACAG+TGG + chr2_2:22336829-22336848 Msa0244150:CDS 35.0%
! ACAGTGGCTTTTGATAAAAC+AGG + chr2_2:22336845-22336864 Msa0244150:CDS 35.0%
!! TCAGTAACAGATTTTAGTGC+TGG + chr2_2:22336890-22336909 Msa0244150:CDS 35.0%
ACAATGAAACCTTACTGTAC+TGG + chr2_2:22336924-22336943 Msa0244150:CDS 35.0%
AATTGATCTCCTGACTCAAA+GGG - chr2_2:22337070-22337089 None:intergenic 35.0%
AAATTGATCTCCTGACTCAA+AGG - chr2_2:22337071-22337090 None:intergenic 35.0%
! TTTCAAAATTTTCCAGGGGA+AGG + chr2_2:22337242-22337261 Msa0244150:CDS 35.0%
GTCAGAGCTATATGTAAAAG+AGG + chr2_2:22337320-22337339 Msa0244150:CDS 35.0%
GTTTCTTGGTAGAAATCTCA+TGG - chr2_2:22337512-22337531 None:intergenic 35.0%
AATTGTGGAGAAACTCTAGT+TGG + chr2_2:22337534-22337553 Msa0244150:CDS 35.0%
! ATGAGTTTTGCCTTTTCATG+TGG - chr2_2:22337806-22337825 None:intergenic 35.0%
TTGCAGGTTATCCATTAGTT+TGG + chr2_2:22338089-22338108 Msa0244150:CDS 35.0%
AAAACAGCTAGCCAAACTAA+TGG - chr2_2:22338103-22338122 None:intergenic 35.0%
AAGTAAGCATGTTCCAACAT+CGG - chr2_2:22338128-22338147 None:intergenic 35.0%
GAAGAAAGAGAATCAACCAA+AGG + chr2_2:22338193-22338212 Msa0244150:CDS 35.0%
AAAGAGAATCAACCAAAGGT+TGG + chr2_2:22338197-22338216 Msa0244150:CDS 35.0%
TCAACCAAAGGTTGGAAATA+TGG + chr2_2:22338205-22338224 Msa0244150:CDS 35.0%
CAAGCAAGGTAATATTGATG+AGG + chr2_2:22338264-22338283 Msa0244150:CDS 35.0%
TTACTCAATGGAAATGACCA+TGG + chr2_2:22338403-22338422 Msa0244150:CDS 35.0%
! AGATTCTAGCATTGCTACTT+TGG + chr2_2:22338477-22338496 Msa0244150:CDS 35.0%
!! GAGAAGCAACTTTGAAGTGT+TGG + chr2_2:22334123-22334142 Msa0244150:CDS 40.0%
!! AGAATTCTGAAGCCTCTTCA+TGG + chr2_2:22334181-22334200 Msa0244150:CDS 40.0%
!!! GAAGCTAGTTTTAGACCACA+AGG + chr2_2:22334591-22334610 Msa0244150:CDS 40.0%
! TCATTATTGGTCTCTCCTTG+TGG - chr2_2:22334609-22334628 None:intergenic 40.0%
TTCTGTTCTCTATTGCTCAG+TGG + chr2_2:22334997-22335016 Msa0244150:CDS 40.0%
TGAGACAAGAGCAACTCATA+AGG + chr2_2:22335022-22335041 Msa0244150:CDS 40.0%
AGAAAGAAAACACAGCGACA+AGG - chr2_2:22335074-22335093 None:intergenic 40.0%
GTACTCTTGCTTCTTGCTTA+AGG + chr2_2:22335197-22335216 Msa0244150:intron 40.0%
AACAAACATGGCTCCTCAAA+TGG + chr2_2:22335415-22335434 Msa0244150:CDS 40.0%
TCAGCAACAATAGCCATTTG+AGG - chr2_2:22335431-22335450 None:intergenic 40.0%
TCCTCTTGATGGAATTGTTG+TGG + chr2_2:22335523-22335542 Msa0244150:CDS 40.0%
TCCACAACAATTCCATCAAG+AGG - chr2_2:22335527-22335546 None:intergenic 40.0%
CTTGATGGAATTGTTGTGGA+AGG + chr2_2:22335527-22335546 Msa0244150:CDS 40.0%
GTTAACAGGAGAATCATTCC+CGG + chr2_2:22335574-22335593 Msa0244150:CDS 40.0%
! AATCTGATTCTTTGGTGACC+GGG - chr2_2:22335595-22335614 None:intergenic 40.0%
! GAATCTGATTCTTTGGTGAC+CGG - chr2_2:22335596-22335615 None:intergenic 40.0%
TGTCCATGGATGTTTATCCT+TGG + chr2_2:22336085-22336104 Msa0244150:intron 40.0%
CACTAATACCGGATGTAATG+TGG - chr2_2:22336161-22336180 None:intergenic 40.0%
CATTACATCCGGTATTAGTG+TGG + chr2_2:22336161-22336180 Msa0244150:intron 40.0%
!! CAAATCATGGTTCTGAATCG+AGG - chr2_2:22336225-22336244 None:intergenic 40.0%
! AGATTAGCCATTGGCAAAGT+TGG - chr2_2:22336282-22336301 None:intergenic 40.0%
GAGTTCATTCACATTGTGTC+AGG + chr2_2:22336384-22336403 Msa0244150:intron 40.0%
!! TACATTGGTTCAAGTGGAAC+TGG - chr2_2:22336419-22336438 None:intergenic 40.0%
ACAAGTGCTAGATGAAACCA+TGG - chr2_2:22336689-22336708 None:intergenic 40.0%
ATGGTTTCATCTAGCACTTG+TGG + chr2_2:22336688-22336707 Msa0244150:CDS 40.0%
!!! GCACTTGTGGTTTTATTGAG+TGG + chr2_2:22336701-22336720 Msa0244150:CDS 40.0%
! TAGAGAGGATAAGAGCACAA+GGG - chr2_2:22336730-22336749 None:intergenic 40.0%
AGAGCACAAAAGATTGCAAC+TGG - chr2_2:22336755-22336774 None:intergenic 40.0%
CTCACAAAAGCTGCAATAAG+TGG + chr2_2:22336773-22336792 Msa0244150:CDS 40.0%
AAAACAGGCACCATAACAAG+AGG + chr2_2:22336860-22336879 Msa0244150:CDS 40.0%
TGAGAACTCTCCTCTTGTTA+TGG - chr2_2:22336873-22336892 None:intergenic 40.0%
! TGAAACCTTACTGTACTGGT+AGG + chr2_2:22336928-22336947 Msa0244150:intron 40.0%
GAAAACCTACCAGTACAGTA+AGG - chr2_2:22336936-22336955 None:intergenic 40.0%
GAGTAAATCAAGTCATCCAG+TGG + chr2_2:22337154-22337173 Msa0244150:CDS 40.0%
CAAACCAGTACCAGAAAATG+TGG + chr2_2:22337214-22337233 Msa0244150:CDS 40.0%
!! TTCTCCACATTTTCTGGTAC+TGG - chr2_2:22337221-22337240 None:intergenic 40.0%
! TTTCCAGGGGAAGGAATTTT+TGG + chr2_2:22337251-22337270 Msa0244150:CDS 40.0%
CTGATCTACAAGCATCAACA+AGG - chr2_2:22337569-22337588 None:intergenic 40.0%
CTTGTTGATGCTTGTAGATC+AGG + chr2_2:22337567-22337586 Msa0244150:CDS 40.0%
TTGTAGATCAGGTGCTTTAG+AGG + chr2_2:22337578-22337597 Msa0244150:CDS 40.0%
ATGGAAGAGCTGAAGTCATT+AGG + chr2_2:22337603-22337622 Msa0244150:CDS 40.0%
TACCTGACTCTGAACATACA+TGG - chr2_2:22337670-22337689 None:intergenic 40.0%
! CTTTTCATGTGGTAGAAGCT+CGG - chr2_2:22337795-22337814 None:intergenic 40.0%
GTTAATGCCATCTCCTATCA+TGG - chr2_2:22337858-22337877 None:intergenic 40.0%
GGCATATCAATGGGAATATC+TGG + chr2_2:22337908-22337927 Msa0244150:CDS 40.0%
! GAATCGCTTCAGGTACTTTT+CGG - chr2_2:22337985-22338004 None:intergenic 40.0%
CAAATGTGCCATACTTGCAT+TGG + chr2_2:22338063-22338082 Msa0244150:CDS 40.0%
TGCAATTGCCAATGCAAGTA+TGG - chr2_2:22338074-22338093 None:intergenic 40.0%
! ATACTTGCATTGGCAATTGC+AGG + chr2_2:22338073-22338092 Msa0244150:CDS 40.0%
!!! CTAGCTGTTTTAACCGATGT+TGG + chr2_2:22338112-22338131 Msa0244150:CDS 40.0%
CTTCTTAGTAGTGAAGAGTG+TGG + chr2_2:22338295-22338314 Msa0244150:CDS 40.0%
!! TTGTGCAACAGAAGCATCAC+TGG + chr2_2:22334153-22334172 Msa0244150:CDS 45.0%
! ACAGCTTTGACTCCATGAAG+AGG - chr2_2:22334196-22334215 None:intergenic 45.0%
ACAACAGTAACTGTTCGAGC+AGG - chr2_2:22334232-22334251 None:intergenic 45.0%
!! ATATCCTCCTTTGGGATGGT+TGG + chr2_2:22334698-22334717 Msa0244150:CDS 45.0%
TACTCCGGCTTCTTGCTTTA+GGG + chr2_2:22335261-22335280 Msa0244150:intron 45.0%
TTTCCCCTAAAGCAAGAAGC+CGG - chr2_2:22335268-22335287 None:intergenic 45.0%
ATGGCTATTGTTGCTGAGAC+CGG + chr2_2:22335434-22335453 Msa0244150:CDS 45.0%
ATTGACATCAACACGTTCCC+CGG - chr2_2:22335456-22335475 None:intergenic 45.0%
GGTGATGCTATTCCTCTTGA+TGG + chr2_2:22335512-22335531 Msa0244150:CDS 45.0%
! TGTGTTGGCGAAAGACACAA+TGG + chr2_2:22335959-22335978 Msa0244150:CDS 45.0%
TGCATGTGCCACACTAATAC+CGG - chr2_2:22336172-22336191 None:intergenic 45.0%
GATTTCAGCTAGCATTGCAG+TGG + chr2_2:22336631-22336650 Msa0244150:CDS 45.0%
TCAGGAACACTTAATGCAGC+TGG - chr2_2:22336659-22336678 None:intergenic 45.0%
TGAAACCATGGCTCCATATC+AGG - chr2_2:22336677-22336696 None:intergenic 45.0%
! GTAGAGAGGATAAGAGCACA+AGG - chr2_2:22336731-22336750 None:intergenic 45.0%
AGATTGCAACTGGTGTAGAG+AGG - chr2_2:22336745-22336764 None:intergenic 45.0%
AAATCAAGTCATCCAGTGGC+AGG + chr2_2:22337158-22337177 Msa0244150:CDS 45.0%
!! GTGCACTAGTGGATTATGCA+AGG + chr2_2:22337180-22337199 Msa0244150:CDS 45.0%
GTACCAAAAATTCCTTCCCC+TGG - chr2_2:22337257-22337276 None:intergenic 45.0%
!! ATCAGGTGCTTTAGAGGCAA+TGG + chr2_2:22337584-22337603 Msa0244150:CDS 45.0%
TGCCATGTATGTTCAGAGTC+AGG + chr2_2:22337665-22337684 Msa0244150:CDS 45.0%
CGAGCTTCTACCACATGAAA+AGG + chr2_2:22337793-22337812 Msa0244150:CDS 45.0%
CCATCTCCTATCATGGCTAT+TGG - chr2_2:22337851-22337870 None:intergenic 45.0%
CCAATAGCCATGATAGGAGA+TGG + chr2_2:22337848-22337867 Msa0244150:CDS 45.0%
ATGTCTGCTGTAGCTAATGC+AGG - chr2_2:22337887-22337906 None:intergenic 45.0%
GCATTAGCTACAGCAGACAT+CGG + chr2_2:22337887-22337906 Msa0244150:CDS 45.0%
AGCAGACATCGGCATATCAA+TGG + chr2_2:22337898-22337917 Msa0244150:CDS 45.0%
GCAGACATCGGCATATCAAT+GGG + chr2_2:22337899-22337918 Msa0244150:CDS 45.0%
CTTGCTAAACGAATCGCTTC+AGG - chr2_2:22337995-22338014 None:intergenic 45.0%
CGGATGCACTGAATACAGCA+AGG + chr2_2:22334565-22334584 Msa0244150:CDS 50.0%
!! GCTCTTGGTTCTGTTGCCAT+TGG + chr2_2:22334720-22334739 Msa0244150:CDS 50.0%
GTACTCCGGCTTCTTGCTTT+AGG + chr2_2:22335260-22335279 Msa0244150:intron 50.0%
ACTCCGGCTTCTTGCTTTAG+GGG + chr2_2:22335262-22335281 Msa0244150:intron 50.0%
TGGCTATTGTTGCTGAGACC+GGG + chr2_2:22335435-22335454 Msa0244150:CDS 50.0%
GAACACCATCCTTGCAGTCA+AGG + chr2_2:22335487-22335506 Msa0244150:CDS 50.0%
TCAGATTCACTTGTCTGGGC+TGG + chr2_2:22335608-22335627 Msa0244150:CDS 50.0%
CCATGGACATGACACATACC+AGG - chr2_2:22336074-22336093 None:intergenic 50.0%
CCTGGTATGTGTCATGTCCA+TGG + chr2_2:22336071-22336090 Msa0244150:intron 50.0%
! CATGCAGCAGAAGCATAGTG+AGG + chr2_2:22336186-22336205 Msa0244150:intron 50.0%
! TGAATCGAGGGCATGTCAGT+CGG - chr2_2:22336212-22336231 None:intergenic 50.0%
TGCCAATGGCTAATCTCCTC+TGG + chr2_2:22336286-22336305 Msa0244150:intron 50.0%
GCCAATGGCTAATCTCCTCT+GGG + chr2_2:22336287-22336306 Msa0244150:intron 50.0%
CCAATGGCTAATCTCCTCTG+GGG + chr2_2:22336288-22336307 Msa0244150:intron 50.0%
ACCTCCTTGGCCACCATTAT+TGG + chr2_2:22336312-22336331 Msa0244150:intron 50.0%
CCTCCTTGGCCACCATTATT+GGG + chr2_2:22336313-22336332 Msa0244150:intron 50.0%
CTCCTTGGCCACCATTATTG+GGG + chr2_2:22336314-22336333 Msa0244150:intron 50.0%
TCCTTGGCCACCATTATTGG+GGG + chr2_2:22336315-22336334 Msa0244150:intron 50.0%
GAAGGGGATACCCCCAATAA+TGG - chr2_2:22336328-22336347 None:intergenic 50.0%
GTGTTCCTGATATGGAGCCA+TGG + chr2_2:22336669-22336688 Msa0244150:CDS 50.0%
CGAAAGAGACCCTTTGAGTC+AGG + chr2_2:22337058-22337077 Msa0244150:intron 50.0%
GTCAGATCAGTCATGCTGAC+AGG + chr2_2:22337627-22337646 Msa0244150:CDS 50.0%
GAAGGGCCAATAGCCATGAT+AGG + chr2_2:22337842-22337861 Msa0244150:CDS 50.0%
AGAGCCAACCATCCCAAAGG+AGG - chr2_2:22334705-22334724 None:intergenic 55.0%
CCAAGAGCCAACCATCCCAA+AGG - chr2_2:22334708-22334727 None:intergenic 55.0%
!! CCTTTGGGATGGTTGGCTCT+TGG + chr2_2:22334705-22334724 Msa0244150:CDS 55.0%
GGCTATTGTTGCTGAGACCG+GGG + chr2_2:22335436-22335455 Msa0244150:CDS 55.0%
ACCATCCTTGCAGTCAAGGC+TGG + chr2_2:22335491-22335510 Msa0244150:CDS 55.0%
ACCAGCCTTGACTGCAAGGA+TGG - chr2_2:22335495-22335514 None:intergenic 55.0%
CATCACCAGCCTTGACTGCA+AGG - chr2_2:22335499-22335518 None:intergenic 55.0%
! CCCCAGAGGAGATTAGCCAT+TGG - chr2_2:22336291-22336310 None:intergenic 55.0%
CCCAATAATGGTGGCCAAGG+AGG - chr2_2:22336316-22336335 None:intergenic 55.0%
ACCCCCAATAATGGTGGCCA+AGG - chr2_2:22336319-22336338 None:intergenic 55.0%
GGGGATACCCCCAATAATGG+TGG - chr2_2:22336325-22336344 None:intergenic 55.0%
! TCTCCTCTGGGGAACCTCCT+TGG + chr2_2:22336299-22336318 Msa0244150:intron 60.0%
TCCAGTGGCAGGTGCACTAG+TGG + chr2_2:22337169-22337188 Msa0244150:CDS 60.0%
TCCACTAGTGCACCTGCCAC+TGG - chr2_2:22337173-22337192 None:intergenic 60.0%
TGGCCAAGGAGGTTCCCCAG+AGG - chr2_2:22336305-22336324 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 22334106 22338528 22334106 ID=Msa0244150;Name=Msa0244150
chr2_2 mRNA 22334106 22338528 22334106 ID=Msa0244150-mRNA-1;Parent=Msa0244150;Name=Msa0244150-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0|0.25|0.11|1|1|1|9|0|808
chr2_2 exon 22334106 22334286 22334106 ID=Msa0244150-mRNA-1:exon:8601;Parent=Msa0244150-mRNA-1
chr2_2 exon 22334565 22334822 22334565 ID=Msa0244150-mRNA-1:exon:8602;Parent=Msa0244150-mRNA-1
chr2_2 exon 22334946 22335043 22334946 ID=Msa0244150-mRNA-1:exon:8603;Parent=Msa0244150-mRNA-1
chr2_2 exon 22335392 22335647 22335392 ID=Msa0244150-mRNA-1:exon:8604;Parent=Msa0244150-mRNA-1
chr2_2 exon 22335937 22336074 22335937 ID=Msa0244150-mRNA-1:exon:8605;Parent=Msa0244150-mRNA-1
chr2_2 exon 22336621 22336945 22336621 ID=Msa0244150-mRNA-1:exon:8606;Parent=Msa0244150-mRNA-1
chr2_2 exon 22337139 22337341 22337139 ID=Msa0244150-mRNA-1:exon:8607;Parent=Msa0244150-mRNA-1
chr2_2 exon 22337484 22337686 22337484 ID=Msa0244150-mRNA-1:exon:8608;Parent=Msa0244150-mRNA-1
chr2_2 exon 22337764 22338528 22337764 ID=Msa0244150-mRNA-1:exon:8609;Parent=Msa0244150-mRNA-1
chr2_2 CDS 22334106 22334286 22334106 ID=Msa0244150-mRNA-1:cds;Parent=Msa0244150-mRNA-1
chr2_2 CDS 22334565 22334822 22334565 ID=Msa0244150-mRNA-1:cds;Parent=Msa0244150-mRNA-1
chr2_2 CDS 22334946 22335043 22334946 ID=Msa0244150-mRNA-1:cds;Parent=Msa0244150-mRNA-1
chr2_2 CDS 22335392 22335647 22335392 ID=Msa0244150-mRNA-1:cds;Parent=Msa0244150-mRNA-1
chr2_2 CDS 22335937 22336074 22335937 ID=Msa0244150-mRNA-1:cds;Parent=Msa0244150-mRNA-1
chr2_2 CDS 22336621 22336945 22336621 ID=Msa0244150-mRNA-1:cds;Parent=Msa0244150-mRNA-1
chr2_2 CDS 22337139 22337341 22337139 ID=Msa0244150-mRNA-1:cds;Parent=Msa0244150-mRNA-1
chr2_2 CDS 22337484 22337686 22337484 ID=Msa0244150-mRNA-1:cds;Parent=Msa0244150-mRNA-1
chr2_2 CDS 22337764 22338528 22337764 ID=Msa0244150-mRNA-1:cds;Parent=Msa0244150-mRNA-1
Gene Sequence

>Msa0244150

ATGGTTGAAAATATAAAGAGAAGCAACTTTGAAGTGTTGGGAATGTGTTGTGCAACAGAAGCATCACTGGTTGAAAGAATTCTGAAGCCTCTTCATGGAGTCAAAGCTGTCTCTGTGATTGTTCCTGCTCGAACAGTTACTGTTGTCCATGATGTTCTCCTTATTTCTGAGTCACAAATAGCGGATGCACTGAATACAGCAAGGCTTGAAGCTAGTTTTAGACCACAAGGAGAGACCAATAATGAAAAGAAATGTCCAGATATACTTACAATGGCATGCGGCTTGTTACTTGCACTTTCATTTTTGAAGTATATATATCCTCCTTTGGGATGGTTGGCTCTTGGTTCTGTTGCCATTGGATTCCCTAAAGTTCTATTTAGGGCTTTAGCTTCCATTAGAGCTCTTACTCTTAATATCAACATTTTGGTTTTATTGGCAGTATGTGGTACTGCTGCTTTACAAGATTTTACAGATGGTGGAGTCATCATCTTTCTGTTCTCTATTGCTCAGTGGCTTGAGACAAGAGCAACTCATAAGGCAATGGTTGCAATGTCGTCTTTAACAAACATGGCTCCTCAAATGGCTATTGTTGCTGAGACCGGGGAACGTGTTGATGTCAATGATGTTAAGATGAACACCATCCTTGCAGTCAAGGCTGGTGATGCTATTCCTCTTGATGGAATTGTTGTGGAAGGAAAGTGTGAAGTTGATGAAAAAATGTTAACAGGAGAATCATTCCCGGTCACCAAAGAATCAGATTCACTTGTCTGGGCTGGAACTATTAATATGAATGGATATATAAGTGTAAGAACAACTGTGTTGGCGAAAGACACAATGGTTGCCCGAATGTCAAGACTTGTTGAAGAAGCTTCTAGCAGAAAATCACAAGCACAAAGATTCATAGATAATTTTGCAAAGTACTATATTCCTGTGGTTGTGTTGATTTCAGCTAGCATTGCAGTGGTTCCAGCTGCATTAAGTGTTCCTGATATGGAGCCATGGTTTCATCTAGCACTTGTGGTTTTATTGAGTGGATGCCCTTGTGCTCTTATCCTCTCTACACCAGTTGCAATCTTTTGTGCTCTCACAAAAGCTGCAATAAGTGGATTACTATTGAAAGGTGGAGATTACCTTGAAACACTGTCTAGAATTAAGACAGTGGCTTTTGATAAAACAGGCACCATAACAAGAGGAGAGTTCTCAGTAACAGATTTTAGTGCTGGAGATGACATCAACAATGAAACCTTACTGTACTGGATTTCGAGTATTGAGAGTAAATCAAGTCATCCAGTGGCAGGTGCACTAGTGGATTATGCAAGGTTGCACTCTATCAAACCAGTACCAGAAAATGTGGAGAATTTTCAAAATTTTCCAGGGGAAGGAATTTTTGGTACAATTGATGGTAATGATATCTATATAGGAAACAAGAGAGTTGGTGTCAGAGCTATATGTAAAAGAGATGATTGCAAAGTGAAAGTTCATAGCCATGAGATTTCTACCAAGAAACAAAATTGTGGAGAAACTCTAGTTGGAGTTTTCAGCCTTGTTGATGCTTGTAGATCAGGTGCTTTAGAGGCAATGGAAGAGCTGAAGTCATTAGGAGTCAGATCAGTCATGCTGACAGGAGATAGCTCTCAAGTTGCCATGTATGTTCAGAGTCAGTTAAACAATGCTATAGACATAGTTCATGCCGAGCTTCTACCACATGAAAAGGCAAAACTCATTGAGAATTTCAAGAAAGAAGGGCCAATAGCCATGATAGGAGATGGCATTAACGATGCTCCTGCATTAGCTACAGCAGACATCGGCATATCAATGGGAATATCTGGTTCAGCTCTTGCAAATGAGACAAGTCACGCGATTCTCATGTCAAACGATATCCGAAAAGTACCTGAAGCGATTCGTTTAGCAAGAAAAACAACTAGAAAACTCATTGAGAATGTCATTATTTCAGTTGGATTCAAATGTGCCATACTTGCATTGGCAATTGCAGGTTATCCATTAGTTTGGCTAGCTGTTTTAACCGATGTTGGAACATGCTTACTTGTGATTTTCAATAGCATGTTAATTTTACAAGAGAATCATAAATATGAAGAAAGAGAATCAACCAAAGGTTGGAAATATGGTAAATTTTTGGAAGATAAGACCAAACCATTACTTAACAAGCAAGGTAATATTGATGAGGAAAAAGGGCTTCTTAGTAGTGAAGAGTGTGGTAAAGATTGTTGTAAAAATGTTACTCATCATGTGGAAACTTCAAACACAAGAAAAGATGAATCTTGTGGAATTTCAAAGTTGAGTTTACTCAATGGAAATGACCATGGAAAGTTTATGTTTGTTGAAGTTCATGTTGTGAAGCCTTGTGTTATGATACAAGATTCTAGCATTGCTACTTTGGAATCTATTACTGTTTCAGAGACAAACTGA
Protein sequence

>Msa0244150

MVENIKRSNFEVLGMCCATEASLVERILKPLHGVKAVSVIVPARTVTVVHDVLLISESQIADALNTARLEASFRPQGETNNEKKCPDILTMACGLLLALSFLKYIYPPLGWLALGSVAIGFPKVLFRALASIRALTLNINILVLLAVCGTAALQDFTDGGVIIFLFSIAQWLETRATHKAMVAMSSLTNMAPQMAIVAETGERVDVNDVKMNTILAVKAGDAIPLDGIVVEGKCEVDEKMLTGESFPVTKESDSLVWAGTINMNGYISVRTTVLAKDTMVARMSRLVEEASSRKSQAQRFIDNFAKYYIPVVVLISASIAVVPAALSVPDMEPWFHLALVVLLSGCPCALILSTPVAIFCALTKAAISGLLLKGGDYLETLSRIKTVAFDKTGTITRGEFSVTDFSAGDDINNETLLYWISSIESKSSHPVAGALVDYARLHSIKPVPENVENFQNFPGEGIFGTIDGNDIYIGNKRVGVRAICKRDDCKVKVHSHEISTKKQNCGETLVGVFSLVDACRSGALEAMEELKSLGVRSVMLTGDSSQVAMYVQSQLNNAIDIVHAELLPHEKAKLIENFKKEGPIAMIGDGINDAPALATADIGISMGISGSALANETSHAILMSNDIRKVPEAIRLARKTTRKLIENVIISVGFKCAILALAIAGYPLVWLAVLTDVGTCLLVIFNSMLILQENHKYEERESTKGWKYGKFLEDKTKPLLNKQGNIDEEKGLLSSEECGKDCCKNVTHHVETSNTRKDESCGISKLSLLNGNDHGKFMFVEVHVVKPCVMIQDSSIATLESITVSETN*