Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0766170 | PNY12771.1 | 81.033 | 1123 | 178 | 4 | 1 | 1095 | 1 | 1116 | 0.0 | 1816 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0766170 | sp|Q7XA39|RGA4_SOLBU | 42.338 | 1018 | 493 | 23 | 1 | 977 | 1 | 965 | 0.0 | 710 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0766170 | A0A2K3PBV7 | 81.033 | 1123 | 178 | 4 | 1 | 1095 | 1 | 1116 | 0.0 | 1816 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000800 | Msa0766170 | 0.811753 | 5.980877e-51 | -8.615850e-47 |
| Msa0004180 | Msa0766170 | -0.827484 | 1.525563e-54 | -8.615850e-47 |
| Msa0006080 | Msa0766170 | 0.815064 | 1.119398e-51 | -8.615850e-47 |
| Msa0011010 | Msa0766170 | 0.837846 | 4.083402e-57 | -8.615850e-47 |
| Msa0011040 | Msa0766170 | 0.835300 | 1.818165e-56 | -8.615850e-47 |
| Msa0011440 | Msa0766170 | -0.812132 | 4.945247e-51 | -8.615850e-47 |
| Msa0013270 | Msa0766170 | 0.857586 | 1.475739e-62 | -8.615850e-47 |
| Msa0016380 | Msa0766170 | -0.803711 | 3.059264e-49 | -8.615850e-47 |
| Msa0017300 | Msa0766170 | -0.819871 | 9.250247e-53 | -8.615850e-47 |
| Msa0017710 | Msa0766170 | 0.830563 | 2.736324e-55 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0766170 | MtrunA17_Chr5g0415471 | 64.399 | 1132 | 359 | 9 | 1 | 1097 | 1 | 1123 | 0.0 | 1425 |
| Msa0766170 | MtrunA17_Chr5g0414311 | 62.014 | 1132 | 389 | 7 | 1 | 1097 | 1 | 1126 | 0.0 | 1379 |
| Msa0766170 | MtrunA17_Chr5g0417251 | 93.617 | 658 | 42 | 0 | 210 | 867 | 1 | 658 | 0.0 | 1259 |
| Msa0766170 | MtrunA17_Chr5g0414181 | 64.395 | 983 | 311 | 7 | 1 | 954 | 1 | 973 | 0.0 | 1234 |
| Msa0766170 | MtrunA17_Chr3g0087301 | 57.193 | 1140 | 358 | 10 | 1 | 1071 | 1 | 1079 | 0.0 | 1201 |
| Msa0766170 | MtrunA17_Chr5g0414201 | 65.190 | 948 | 293 | 5 | 1 | 919 | 1 | 940 | 0.0 | 1188 |
| Msa0766170 | MtrunA17_Chr6g0470011 | 41.337 | 1137 | 569 | 21 | 1 | 1078 | 1 | 1098 | 0.0 | 795 |
| Msa0766170 | MtrunA17_Chr6g0470081 | 41.316 | 1140 | 565 | 24 | 1 | 1078 | 1 | 1098 | 0.0 | 784 |
| Msa0766170 | MtrunA17_Chr3g0123111 | 40.231 | 1126 | 582 | 18 | 1 | 1071 | 1 | 1090 | 0.0 | 764 |
| Msa0766170 | MtrunA17_Chr3g0085931 | 40.422 | 1138 | 581 | 21 | 1 | 1076 | 1 | 1103 | 0.0 | 761 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0766170 | AT3G14470.1 | 30.624 | 1009 | 588 | 27 | 34 | 973 | 42 | 1007 | 9.14e-123 | 403 |
| Msa0766170 | AT3G14470.2 | 30.624 | 1009 | 588 | 27 | 34 | 973 | 42 | 1007 | 9.14e-123 | 403 |
| Msa0766170 | AT3G14460.1 | 30.603 | 879 | 514 | 25 | 31 | 838 | 36 | 889 | 8.54e-96 | 334 |
| Msa0766170 | AT3G50950.2 | 27.000 | 800 | 505 | 23 | 1 | 750 | 1 | 771 | 5.83e-57 | 213 |
| Msa0766170 | AT3G50950.1 | 27.000 | 800 | 505 | 23 | 1 | 750 | 1 | 771 | 5.83e-57 | 213 |
| Msa0766170 | AT3G46530.1 | 25.921 | 706 | 432 | 19 | 1 | 640 | 1 | 681 | 1.99e-47 | 184 |
| Msa0766170 | AT3G07040.1 | 24.380 | 685 | 417 | 21 | 1 | 614 | 1 | 655 | 3.17e-47 | 184 |
| Msa0766170 | AT3G46730.1 | 24.612 | 837 | 488 | 25 | 1 | 756 | 1 | 775 | 6.43e-43 | 169 |
| Msa0766170 | AT3G46710.1 | 23.885 | 695 | 429 | 23 | 1 | 645 | 1 | 645 | 2.92e-38 | 155 |
| Msa0766170 | AT5G35450.2 | 25.678 | 701 | 407 | 23 | 1 | 640 | 1 | 648 | 4.32e-36 | 148 |
Find 237 sgRNAs with CRISPR-Local
Find 259 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGAATGGATCTATGTCA+AGG | 0.585064 | 5_2:-27609217 | Msa0766170:CDS |
| AAAAGCCTACAAAGAGATTC+TGG | 0.304683 | 5_2:+27608553 | None:intergenic |
| AAAAGGAAATAGGCGAGGAC+TGG | 0.530743 | 5_2:-27607127 | Msa0766170:CDS |
| AAACAAGGATATGGTGGAAG+TGG | 0.541890 | 5_2:-27607062 | Msa0766170:CDS |
| AAACCTGATAAGGCTATCAA+GGG | 0.612126 | 5_2:-27607867 | Msa0766170:CDS |
| AAATACAAATTCTTGAGAGA+TGG | 0.537084 | 5_2:+27607764 | None:intergenic |
| AACAAATTGAGGTTGTCATG+GGG | 0.584455 | 5_2:-27608223 | Msa0766170:CDS |
| AACAAGCTTGGCCTATTTCT+TGG | 0.286522 | 5_2:-27610316 | Msa0766170:CDS |
| AACATTGAAAGTTCATGAGC+AGG | 0.553850 | 5_2:+27609405 | None:intergenic |
| AACCCTATATGGAGTACCCT+AGG | 0.566175 | 5_2:+27607009 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CAAACATAGCTATAAAGGAT+TGG | - | chr5_2:27607173-27607192 | Msa0766170:CDS | 30.0% | |
| TTGAAGTATGAAAGACACAA+GGG | - | chr5_2:27607268-27607287 | Msa0766170:CDS | 30.0% | |
| AGAAAAGAGCAAGTTTCATT+TGG | - | chr5_2:27607414-27607433 | Msa0766170:CDS | 30.0% | |
| TCATTTCTTCCATATACTTG+AGG | + | chr5_2:27607508-27607527 | None:intergenic | 30.0% | |
| TTATCGGTCTATCCATTAAT+AGG | - | chr5_2:27607583-27607602 | Msa0766170:CDS | 30.0% | |
| GTCTATCCATTAATAGGTAT+AGG | - | chr5_2:27607589-27607608 | Msa0766170:CDS | 30.0% | |
| TATCCATTAATAGGTATAGG+TGG | - | chr5_2:27607592-27607611 | Msa0766170:CDS | 30.0% | |
| AGAATGACAAAAGCTATCAT+AGG | - | chr5_2:27607723-27607742 | Msa0766170:CDS | 30.0% | |
| TTGTTTAAACAACGAGCATT+TGG | - | chr5_2:27608023-27608042 | Msa0766170:CDS | 30.0% | |
| AAAAGAATGGATCTATGTCA+AGG | - | chr5_2:27608166-27608185 | Msa0766170:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 27607013 | 27610392 | 27607013 | ID=Msa0766170;Name=Msa0766170 |
| chr5_2 | mRNA | 27607013 | 27610392 | 27607013 | ID=Msa0766170-mRNA-1;Parent=Msa0766170;Name=Msa0766170-mRNA-1;_AED=0.17;_eAED=0.17;_QI=0|0|0|0.5|1|1|2|0|1097 |
| chr5_2 | exon | 27609786 | 27610392 | 27609786 | ID=Msa0766170-mRNA-1:exon:10814;Parent=Msa0766170-mRNA-1 |
| chr5_2 | exon | 27607013 | 27609699 | 27607013 | ID=Msa0766170-mRNA-1:exon:10813;Parent=Msa0766170-mRNA-1 |
| chr5_2 | CDS | 27609786 | 27610392 | 27609786 | ID=Msa0766170-mRNA-1:cds;Parent=Msa0766170-mRNA-1 |
| chr5_2 | CDS | 27607013 | 27609699 | 27607013 | ID=Msa0766170-mRNA-1:cds;Parent=Msa0766170-mRNA-1 |
| Gene Sequence |
>Msa0766170 ATGGCTGAGGCTGTGGTCGAATTTGTGCTTAAGAATTTGACCTCACTCATTGCAAACAAGCTTGGCCTATTTCTTGGTTTCGATAAGGACTTTAAAAGTCTTGCTAGCTTGCTCACTACGATACAGGCTACACTTGAAGACGCTGAGGAGAAACAATTCTCAAACATAGCTATAAAGGATTGGTTGCAAAAGCTGAAAGATGCTGCTCACGTTCTAGATGATATTTTGGACGAGTGTGCCGCTGAAGCATCGGAGTTGAAGTATGAAAGACACAAGGGTGGACCATCCGAGAAGGTACAAAGCTCTTTCTTGTCCTCTTATCACCCTAAGCAGGTTGCTTTCCGTTACAAAATTGCTAAGAAAATGAAAAAGATAAGGGAAAGATTAAATGAAATTAGCGAAGAAAAGAGCAAGTTTCATTTGGCAGAGACCGATAGGAAGAGACAAAATGGAGTCATTGACTGGCGTGAAACCACCTCAATCATCACTCAACCTCAAGTATATGGAAGAAATGAAGATAGGGATAAAATTGTAGACTTTTTTGTTAGTGATGCATGTGAATCGGAGGATTTATCGGTCTATCCATTAATAGGTATAGGTGGACTTGAAGATTTCAGTATGAAGAGAATGACAAAAGCTATCATAGGATCAACGTCTGGAAATTCCTATGACTGTGAGGATTTAGATCTAGAGCCCCTACAAAGAAGATTGCAAGAGCTACTTCGAAGAAAAAGATATCTGCTTGTTTTGGATGATTTATGGGATGAGGAACAAGAGAATTGGCTGAAGTTGAAGTCTGTATTGGCTTGTGGAGGAAAAGGTGCTTCAATTTTGGTCACTACACGGCTTCCAAAGGTAGCAGAAATCATGGGAACGGTGCCTGCTCATGAACTTTCAATGTTGTCTGACAAAGACTGCTGGGAATTGTTTAAACAACGAGCATTTGGACCAAATGAGGTAGAACGGACAAAGCTTGTGGAAATTGGGAAAGAGATCCTAAAGAAGTGTAGGGGAGTGCCTCTAGCAGCAATAACATTAGGAAGTCTGTTGCGCTTCAAAAGAGAAGAAAAAGAATGGATCTATGTCAAGGACAGCAAGTTGTGGAGCTTACAAGGTGAGAACTCTGTCATGCAGGCCTTGCGATTAAGTTACTTGTACTTGCCAGTTAAGTTGAGACAATGCTTTGCCTTCTGTGCTATATTTCCAAAAGATGAATTAATAAGTAAGCAACTTCTAATTGAGCTTTGGGTGGCCAATGGATTCATTTCGTCTAATGAAAGTTTGGAAGTGGAAGATATTGGTGATGAGGTGTGGAATGAATTATATTGGAGTTCACTTTTTCAAGATGTTCAAACGGATAAATTAGGCAAGGTTACATATTTCAAAATGCATGATCTTGTTCATGATCTTGCTCAATCTTTTGCAGAGGAAATTTGTTGCAGTGCATATAATAATGGTATAATTAATATGCATGCAAGAATCCGTCACTTCTCAGTTTACGGACAACACAGATCTGAAGATTTCAGTTCAATCCAGTTTCACCATATCAACTCCTTGAAGACCTACATAGAGTGGAATTTCAATCATGCTGGCCAACTTTCGCCTCAAATATTGAAATGTTATTCTTTAAGGGTGCTTCGGAGCAACAACCTAGACATTTTGTCAGCTTCGATTGATCGTTTAAAATATTTAAGATACTTAGATATTTCTCATGGAAGATTCAGAACTCTTCCAGAATCTCTTTGTAGGCTTTTCAATTTGCAGGTTTTGAAATTAGACCATTGTTATGATCTACAAAGTTTGCCTGATAGTTTGGCACACTTAAAATCTCTACAACAACTATCTTTGAGAGCTTGCCACTCACTATCAAGTTTGCCCCCTAAGATAGGAACGTTGACTTCCCTAAGGACTTTAAGCATATATGTTGTTGGTAAGAAAAGAGGGTACCTTTTGGAAGAATTAGGACAACTAAACCTTAAAGGAGAACTTCACATCAAGCACCTAGAGAGAGTAAAAAGTGTAACACATGCCAAAGAAGCCAATATGTCTAGCAAACACTTGAACAAATTGAGGTTGTCATGGGGGAGAAATGAAGAGTCCCAATTACAAGGAAATGTTGAGCAGATTCTCGAAGTGCTTCAACCTCACACCGAACAACTTGATTCTTTGGGTTTGGGAGGGTATACAGGTACATATTTCCCACAGTGGATGTCTAGTCCTTCTCTAAAAGGTTTAACTTCTTTAGAGCTCACAGATTGCAAAAATTGTTTGCACCTTCCGAAGCTCGGGAAATTATCTTCTTTAAAGAATCTAAAAATATCTAACATGAGTCATGTAATATACCTATGGGAGGAGTCCTGTAATGGTGGAGTTGGAGGTTTAATGGCTTTGGAAGCTCTTATTCTAGATAAGCTGCCAAACCTGATAAGGCTATCAAGGGAAGATGGAGAAAATATGTTCATGACCCTTAGTGTACTTCAAATAACTGAATGTCCTAATTTTTCTGGTTTGCCTTGTCTTCCATCTCTCAAGAATTTGTATTTGTATACAGAAGGGAAATACAATCAAGATTTACTAAGATCAGTTAAAAAATTAGATAGTCTTGAAACGCTGCATTTTTTGAAGAATGATGAGTTAACTTACTTTCCAGATGAGATCTTACTAAACCTTGCATCTATAAGGACATTAGGCTTTCATCACCACTCCAAACTTGAGGTGCTCCCAAATGAAATCATTAACCTTCATGCTCTCCAACACCTGTATATTACCAACTGTGTCACTATTGAGTCATTGACAGATGAAGTATTGAAAGGACTATCCTCTCTCAAGTTGCTAGAGATAGTGAAGTGCCACAAGTTGAATCTGTCAGAAGGTTTTCAATATCTAACTAGTCTAGAGGCATTGGTGATTGCTAGCTGTTCGGAAGTAGAAAGTTTTCACCAGGCTTTGCAACATATGACTTCTCTGCAATGCATAATATTGTCTGAACTTCCAAAACTAGAATATTTGCCTGATTGGTTGGGAAACCTTTCCTTACTTCAGGAATTAATTATTGCTCTGTGTCCTAATTTGATCTATCTTCCAGCGAGCATCAAATGTCTTAGCAGTTTGAAAAGATTGTGCATTCAAAGCTGCCCTCATATAGAGAAGCGATGCAAAAAGGAAATAGGCGAGGACTGGTCAAAAATAGCCCATGTTCAGCGTATTGAAATAGGAAGTGATAAACAAGGATATGGTGGAAGTGGTGGTCGATATTATGGGGCCAATGCAGGTTTCCTAGGGTACTCCATATAG |
| Protein sequence |
>Msa0766170 MAEAVVEFVLKNLTSLIANKLGLFLGFDKDFKSLASLLTTIQATLEDAEEKQFSNIAIKDWLQKLKDAAHVLDDILDECAAEASELKYERHKGGPSEKVQSSFLSSYHPKQVAFRYKIAKKMKKIRERLNEISEEKSKFHLAETDRKRQNGVIDWRETTSIITQPQVYGRNEDRDKIVDFFVSDACESEDLSVYPLIGIGGLEDFSMKRMTKAIIGSTSGNSYDCEDLDLEPLQRRLQELLRRKRYLLVLDDLWDEEQENWLKLKSVLACGGKGASILVTTRLPKVAEIMGTVPAHELSMLSDKDCWELFKQRAFGPNEVERTKLVEIGKEILKKCRGVPLAAITLGSLLRFKREEKEWIYVKDSKLWSLQGENSVMQALRLSYLYLPVKLRQCFAFCAIFPKDELISKQLLIELWVANGFISSNESLEVEDIGDEVWNELYWSSLFQDVQTDKLGKVTYFKMHDLVHDLAQSFAEEICCSAYNNGIINMHARIRHFSVYGQHRSEDFSSIQFHHINSLKTYIEWNFNHAGQLSPQILKCYSLRVLRSNNLDILSASIDRLKYLRYLDISHGRFRTLPESLCRLFNLQVLKLDHCYDLQSLPDSLAHLKSLQQLSLRACHSLSSLPPKIGTLTSLRTLSIYVVGKKRGYLLEELGQLNLKGELHIKHLERVKSVTHAKEANMSSKHLNKLRLSWGRNEESQLQGNVEQILEVLQPHTEQLDSLGLGGYTGTYFPQWMSSPSLKGLTSLELTDCKNCLHLPKLGKLSSLKNLKISNMSHVIYLWEESCNGGVGGLMALEALILDKLPNLIRLSREDGENMFMTLSVLQITECPNFSGLPCLPSLKNLYLYTEGKYNQDLLRSVKKLDSLETLHFLKNDELTYFPDEILLNLASIRTLGFHHHSKLEVLPNEIINLHALQHLYITNCVTIESLTDEVLKGLSSLKLLEIVKCHKLNLSEGFQYLTSLEALVIASCSEVESFHQALQHMTSLQCIILSELPKLEYLPDWLGNLSLLQELIIALCPNLIYLPASIKCLSSLKRLCIQSCPHIEKRCKKEIGEDWSKIAHVQRIEIGSDKQGYGGSGGRYYGANAGFLGYSI* |
