AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0248810

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0248810 MtrunA17_Chr2g0304061 94.498 309 14 2 8 315 22 328 8.13e-145 410
Msa0248810 MtrunA17_Chr3g0096451 51.799 139 60 3 91 227 62 195 4.60e-35 127
Msa0248810 MtrunA17_Chr5g0443331 49.655 145 47 4 95 234 75 198 5.28e-33 122
Msa0248810 MtrunA17_Chr7g0271411 61.905 84 31 1 145 227 160 243 2.38e-31 119
Msa0248810 MtrunA17_Chr1g0193131 64.634 82 29 0 142 223 417 498 6.36e-31 122
Msa0248810 MtrunA17_Chr8g0378361 48.031 127 61 2 138 263 23 145 2.34e-29 110
Msa0248810 MtrunA17_Chr8g0381081 56.322 87 36 2 145 229 57 143 1.31e-26 103
Msa0248810 MtrunA17_Chr8g0380231 60.000 80 28 2 154 232 26 102 6.49e-26 99.0
Msa0248810 MtrunA17_Chr5g0447651 56.471 85 35 2 145 227 30 114 6.00e-25 97.1
Msa0248810 MtrunA17_Chr4g0075031 54.348 92 40 2 145 234 382 473 1.20e-24 103
Msa0248810 MtrunA17_Chr8g0378371 54.945 91 39 1 140 230 28 116 3.05e-24 95.1
Msa0248810 MtrunA17_Chr8g0378621 48.598 107 53 2 123 227 342 448 2.39e-23 100
Msa0248810 MtrunA17_Chr4g0040911 42.424 132 65 4 135 262 25 149 1.29e-22 92.8
Msa0248810 MtrunA17_Chr8g0378381 48.810 84 42 1 145 227 32 115 1.79e-22 90.5
Msa0248810 MtrunA17_Chr1g0213651 48.810 84 42 1 145 227 276 359 6.92e-22 94.7
Msa0248810 MtrunA17_Chr1g0177171 52.273 88 40 2 142 227 403 490 7.20e-22 95.5
Msa0248810 MtrunA17_Chr4g0076131 47.253 91 46 2 140 228 23 113 2.50e-21 87.4
Msa0248810 MtrunA17_Chr1g0154991 45.882 85 45 1 145 228 20 104 1.41e-20 85.1
Msa0248810 MtrunA17_Chr8g0378401 51.282 78 37 1 145 221 32 109 1.59e-20 85.5
Msa0248810 MtrunA17_Chr3g0121271 43.119 109 60 2 128 234 345 453 2.84e-20 90.9
Msa0248810 MtrunA17_Chr5g0401301 51.685 89 40 3 145 230 329 417 3.09e-19 87.4
Msa0248810 MtrunA17_Chr5g0419121 49.315 73 36 1 142 213 19 91 3.71e-19 85.1
Msa0248810 MtrunA17_Chr1g0161011 50.000 88 42 2 145 230 361 448 4.19e-19 87.4
Msa0248810 MtrunA17_Chr8g0389741 48.235 85 42 2 145 227 368 452 7.43e-19 87.0
Msa0248810 MtrunA17_Chr4g0076141 50.000 80 38 2 144 221 12 91 8.66e-19 80.1
Msa0248810 MtrunA17_Chr5g0439251 45.455 99 44 3 142 230 379 477 8.72e-19 86.7
Msa0248810 MtrunA17_Chr6g0487261 43.820 89 47 2 142 227 366 454 8.90e-19 86.7
Msa0248810 MtrunA17_Chr4g0034901 46.154 91 47 2 144 232 374 464 4.98e-18 84.3
Msa0248810 MtrunA17_Chr4g0043511 42.391 92 52 1 144 234 419 510 5.75e-18 84.3
Msa0248810 MtrunA17_Chr5g0434591 48.193 83 41 2 145 225 360 442 1.06e-17 83.6
Msa0248810 MtrunA17_Chr3g0110371 44.000 100 54 2 130 227 346 445 1.35e-17 83.2
Msa0248810 MtrunA17_Chr4g0061401 45.238 84 44 2 145 227 24 106 9.05e-17 77.8
Msa0248810 MtrunA17_Chr4g0043151 49.296 71 35 1 145 214 21 91 2.65e-16 75.9
Msa0248810 MtrunA17_Chr3g0144531 35.652 115 60 3 121 232 356 459 6.47e-15 75.1
Msa0248810 MtrunA17_Chr7g0234821 43.182 88 48 2 142 227 371 458 9.90e-15 74.3
Msa0248810 MtrunA17_Chr8g0339191 39.773 88 51 2 142 227 371 458 1.89e-14 73.6
Msa0248810 MtrunA17_Chr7g0223961 37.209 86 53 1 143 227 363 448 4.34e-13 69.3
Msa0248810 MtrunA17_Chr4g0046311 43.902 82 44 2 142 221 370 451 6.62e-13 68.9
Msa0248810 MtrunA17_Chr2g0313891 41.573 89 50 2 141 228 26 113 1.75e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0248810 AT2G30933.1 58.947 95 38 1 141 234 78 172 2.55e-34 125
Msa0248810 AT1G09460.1 59.140 93 37 1 142 234 134 225 2.13e-30 117
Msa0248810 AT2G30933.2 60.976 82 31 1 141 221 78 159 3.43e-30 112
Msa0248810 AT2G30933.3 60.976 82 31 1 141 221 78 159 3.43e-30 112
Msa0248810 AT5G35740.1 52.809 89 37 4 144 228 29 116 1.14e-24 96.7
Msa0248810 AT4G13600.1 43.411 129 60 3 142 257 58 186 3.38e-24 98.6
Msa0248810 AT4G05430.1 47.312 93 47 2 142 232 20 112 2.42e-23 94.0
Msa0248810 AT4G05430.3 47.312 93 47 2 142 232 45 137 3.22e-23 94.4
Msa0248810 AT4G26830.2 47.115 104 53 2 126 227 352 455 7.39e-23 98.6
Msa0248810 AT4G26830.1 47.115 104 53 2 126 227 348 451 9.10e-23 98.6
Msa0248810 AT1G11820.2 49.000 100 49 2 145 242 381 480 4.41e-22 96.7
Msa0248810 AT2G05790.1 51.546 97 45 2 133 227 372 468 7.53e-22 95.9
Msa0248810 AT5G63250.1 46.154 91 45 2 143 229 40 130 9.04e-22 89.4
Msa0248810 AT2G42930.1 47.727 88 45 1 145 232 40 126 1.27e-21 89.0
Msa0248810 AT4G34480.3 47.619 84 43 1 145 227 253 336 2.15e-21 94.0
Msa0248810 AT4G34480.1 47.619 84 43 1 145 227 364 447 2.25e-21 94.7
Msa0248810 AT1G18650.1 45.161 93 50 1 139 230 15 107 3.66e-21 89.4
Msa0248810 AT5G67460.1 48.889 90 44 2 143 230 291 380 6.69e-21 92.4
Msa0248810 AT5G55180.1 50.000 88 42 2 142 227 369 456 8.54e-21 92.8
Msa0248810 AT1G79480.2 46.429 84 44 1 145 227 310 393 8.79e-21 92.4
Msa0248810 AT1G79480.1 46.429 84 44 1 145 227 311 394 9.49e-21 92.0
Msa0248810 AT1G18650.2 42.991 107 55 3 138 242 14 116 2.58e-20 85.5
Msa0248810 AT2G01630.1 50.588 85 40 2 145 227 360 444 3.12e-20 91.3
Msa0248810 AT1G66250.1 49.438 89 43 2 145 231 368 456 4.83e-20 90.9
Msa0248810 AT3G58100.1 47.191 89 45 2 145 231 41 129 7.58e-20 85.9
Msa0248810 AT2G04910.2 47.727 88 44 2 143 228 13 100 8.80e-20 83.2
Msa0248810 AT4G05430.2 49.367 79 38 2 145 221 48 126 9.05e-20 84.0
Msa0248810 AT5G24318.3 36.752 117 65 2 115 230 211 319 1.90e-19 87.4
Msa0248810 AT5G24318.1 39.785 93 55 1 139 230 366 458 2.55e-19 88.6
Msa0248810 AT3G58100.2 45.918 98 45 3 142 231 108 205 4.28e-19 85.5
Msa0248810 AT4G29360.2 47.727 88 44 2 142 227 398 485 9.08e-19 87.0
Msa0248810 AT4G29360.1 47.727 88 44 2 142 227 388 475 1.07e-18 87.0
Msa0248810 AT1G66855.1 43.023 86 47 2 145 228 26 111 1.15e-18 80.5
Msa0248810 AT5G63225.1 44.706 85 46 1 144 227 24 108 1.22e-18 80.5
Msa0248810 AT1G66870.1 44.578 83 44 2 145 225 26 108 1.55e-18 80.1
Msa0248810 AT5G56590.1 47.059 85 43 2 145 227 369 453 1.99e-18 85.9
Msa0248810 AT5G67460.2 50.617 81 38 2 143 221 291 371 2.01e-18 85.5
Msa0248810 AT1G66870.2 44.578 83 44 2 145 225 53 135 2.52e-18 80.5
Msa0248810 AT2G16230.1 42.857 84 47 1 145 227 366 449 3.28e-18 85.5
Msa0248810 AT1G66852.1 42.683 82 46 1 145 225 26 107 3.73e-18 79.0
Msa0248810 AT3G55430.1 36.111 108 60 2 129 227 342 449 3.92e-18 85.1
Msa0248810 AT3G13560.4 42.105 114 62 3 141 252 358 469 3.94e-18 85.1
Msa0248810 AT3G13560.1 42.105 114 62 3 141 252 358 469 3.94e-18 85.1
Msa0248810 AT3G13560.2 42.105 114 62 3 141 252 358 469 3.94e-18 85.1
Msa0248810 AT3G13560.5 42.105 114 62 3 141 252 358 469 3.94e-18 85.1
Msa0248810 AT3G13560.3 42.105 114 62 3 141 252 358 469 3.94e-18 85.1
Msa0248810 AT5G63240.1 42.857 91 48 2 143 229 38 128 4.69e-18 79.3
Msa0248810 AT1G11820.3 51.899 79 36 2 145 221 381 459 8.37e-18 84.0
Msa0248810 AT2G01630.3 50.633 79 37 2 145 221 360 438 9.24e-18 84.0
Msa0248810 AT5G55180.2 50.602 83 39 2 142 222 369 451 9.66e-18 84.0
Msa0248810 AT5G61130.1 40.800 125 72 2 139 262 15 138 1.37e-17 80.1
Msa0248810 AT1G26450.1 49.296 71 34 2 146 215 22 91 2.68e-16 76.3
Msa0248810 AT1G69295.1 50.704 71 33 2 145 214 21 90 3.35e-16 76.6
Msa0248810 AT1G69295.2 50.704 71 33 2 145 214 17 86 5.61e-16 75.9
Msa0248810 AT5G24318.2 40.476 84 49 1 139 221 366 449 6.29e-16 78.6
Msa0248810 AT4G09462.1 37.209 86 53 1 145 229 29 114 7.40e-16 72.8
Msa0248810 AT2G04910.1 46.914 81 41 2 143 221 13 93 1.93e-15 72.0
Msa0248810 AT4G09465.1 36.047 86 54 1 145 229 31 116 3.05e-15 71.2
Msa0248810 AT4G09464.1 36.047 86 54 1 145 229 31 116 3.41e-15 71.2
Msa0248810 AT4G09467.1 36.047 86 54 1 145 229 31 116 3.41e-15 71.2
Msa0248810 AT5G08000.2 42.529 87 49 1 142 227 37 123 7.39e-15 72.8
Msa0248810 AT3G07320.1 44.318 88 47 2 142 227 368 455 8.24e-15 75.1
Msa0248810 AT5G08000.1 42.857 84 47 1 145 227 21 104 1.03e-14 72.0
Msa0248810 AT5G63230.1 39.759 83 49 1 144 225 25 107 1.38e-14 69.3
Msa0248810 AT3G23770.1 43.182 88 48 2 142 227 384 471 2.09e-14 73.9
Msa0248810 AT4G16165.1 38.372 86 51 2 145 228 26 111 4.89e-14 67.8
Msa0248810 AT4G09090.1 36.782 87 54 1 144 229 30 116 9.81e-14 67.0
Msa0248810 AT2G03505.4 34.021 97 47 2 145 241 21 100 3.05e-13 67.0
Msa0248810 AT2G03505.1 34.021 97 47 2 145 241 21 100 3.05e-13 67.0
Msa0248810 AT2G03505.2 34.021 97 47 2 145 241 21 100 3.05e-13 67.0
Msa0248810 AT4G09466.1 33.721 86 56 1 145 229 31 116 3.86e-13 65.5
Msa0248810 AT2G29735.1 43.529 85 45 2 144 225 30 114 5.73e-13 65.1
Msa0248810 AT2G03505.3 34.021 97 47 2 145 241 21 100 6.11e-13 65.1
Msa0248810 AT2G19440.2 42.045 88 43 5 144 227 361 444 1.14e-12 68.6
Msa0248810 AT2G19440.1 42.045 88 43 5 144 227 361 444 1.14e-12 68.6
Msa0248810 AT5G53600.1 39.286 84 49 2 145 227 29 111 1.41e-12 63.9
Msa0248810 AT5G53610.1 41.667 84 47 2 145 227 28 110 1.70e-12 63.5
Msa0248810 AT2G39640.1 34.524 84 54 1 143 225 458 541 3.11e-12 67.4
Msa0248810 AT2G39640.1 35.714 84 53 1 143 225 357 440 1.66e-11 65.5
Msa0248810 AT1G13830.2 39.726 73 43 1 142 213 32 104 7.34e-12 64.3
Msa0248810 AT1G13830.1 39.437 71 42 1 145 214 21 91 1.62e-11 62.8
Msa0248810 AT1G78520.1 38.824 85 50 2 145 228 32 115 3.55e-11 60.1
Msa0248810 AT1G64760.1 39.773 88 45 5 144 227 365 448 7.95e-11 63.2
Msa0248810 AT1G64760.2 39.773 88 45 5 144 227 365 448 7.95e-11 63.2

Find 116 sgRNAs with CRISPR-Local

Find 274 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATGATCTTTCAAAGTATT+TGG 0.103855 2_2:+33582410 None:intergenic
GGGCTTGTTGTGCCTGTGTC+TGG 0.191361 2_2:+33582761 None:intergenic
TTTAGCATTAGCAGATTTAT+AGG 0.211938 2_2:-33581473 Msa0248810:three_prime_UTR
CCTCCTGTTCCTGGTGTTGT+AGG 0.230252 2_2:+33582857 None:intergenic
AAATTCTCACCTTGTTGTTT+TGG 0.240571 2_2:+33582015 None:intergenic
AGAAGTTGATGTAGCTATAT+TGG 0.257371 2_2:+33581754 None:intergenic
TTGGATTATGCTTGTGGATA+TGG 0.261053 2_2:-33582476 Msa0248810:CDS
GCACCTACAAGTTGTGTATT+TGG 0.264956 2_2:-33582350 Msa0248810:CDS
AGATTATCTATTTCAGATAA+AGG 0.268892 2_2:-33581401 Msa0248810:three_prime_UTR
ATAAATCTGCTAATGCTAAA+TGG 0.271823 2_2:+33581476 None:intergenic
AGCTGCAGAGACTACTTTAA+AGG 0.280134 2_2:-33582504 Msa0248810:CDS
TGAATCTTCTCTTAATCTTA+TGG 0.280733 2_2:+33582928 None:intergenic
GGGCTTGTTGGGTTTGAGTA+TGG 0.288454 2_2:+33582641 None:intergenic
GAAGTTGATGTAGCTATATT+GGG 0.302042 2_2:+33581755 None:intergenic
GACTACTTTAAAGGTAGCTT+TGG 0.305217 2_2:-33582495 Msa0248810:CDS
CTTGTTGGAGTTGTTGTAGT+TGG 0.311943 2_2:+33582620 None:intergenic
GCATAGCTATTGATTGAATA+TGG 0.315916 2_2:-33581329 Msa0248810:three_prime_UTR
GGTGTTGTGGGAGTCACATT+TGG 0.328603 2_2:+33582596 None:intergenic
GAACTTGGTGGGTTTGAGTA+TGG 0.330703 2_2:+33582671 None:intergenic
GTTGTGCCTGTGTCTGGGCT+TGG 0.333394 2_2:+33582767 None:intergenic
GATAACTAAATATTATTTCA+TGG 0.335055 2_2:+33581297 None:intergenic
ATGACATGTTCTACAGGTTT+AGG 0.336717 2_2:-33582956 Msa0248810:CDS
CATGATCTTTCAAAGTATTT+GGG 0.337641 2_2:+33582411 None:intergenic
TAAGGATTTCCTCCTGTTCC+TGG 0.340078 2_2:+33582848 None:intergenic
GTTGTTGTAGTTGGGCTTGT+TGG 0.343594 2_2:+33582629 None:intergenic
GATTATCTATTTCAGATAAA+GGG 0.350069 2_2:-33581400 Msa0248810:three_prime_UTR
TGGAATTCACACAGTCTTCC+AGG 0.351476 2_2:-33581605 Msa0248810:three_prime_UTR
GGCTTGTTGTGCCTGTGTCT+GGG 0.359856 2_2:+33582762 None:intergenic
GAAGCTGGTGGGTTTGAATA+TGG 0.370833 2_2:+33582701 None:intergenic
CCTCCAAATACACAACTTGT+AGG 0.370834 2_2:+33582347 None:intergenic
GTGCCTGTGTCTGGGCTTGG+AGG 0.373150 2_2:+33582770 None:intergenic
GAGGTTGGTGGATTTGAGTA+TGG 0.378917 2_2:+33582731 None:intergenic
GGATTTGAGTATGGATTTGT+TGG 0.380426 2_2:+33582740 None:intergenic
TTGTTGTAGTTGGGCTTGTT+GGG 0.380851 2_2:+33582630 None:intergenic
GGAGATGTTGGGTTTATAGT+AGG 0.382143 2_2:+33582824 None:intergenic
GGAGTCACATTTGGGCTTGT+TGG 0.384264 2_2:+33582605 None:intergenic
TGCCTGTGTCTGGGCTTGGA+GGG 0.391683 2_2:+33582771 None:intergenic
GTTGGGTTTATAGTAGGATA+AGG 0.392906 2_2:+33582830 None:intergenic
CCACCAGATGATCCTGTTCC+TGG 0.395410 2_2:+33582575 None:intergenic
ATATTACACCGACCCTTGAT+TGG 0.395924 2_2:-33581625 Msa0248810:three_prime_UTR
TCAGGTGGTTGAGGAGATGT+TGG 0.398484 2_2:+33582812 None:intergenic
CTTAGTTGCAAGAATTCATA+TGG 0.399617 2_2:+33581536 None:intergenic
GGATCATCTGGTGGTGGTGG+TGG 0.399836 2_2:-33582566 Msa0248810:CDS
GCTATATTGGGGCTTCCTCC+TGG 0.414020 2_2:+33581767 None:intergenic
GTGTTGTGGGAGTCACATTT+GGG 0.421820 2_2:+33582597 None:intergenic
CTCCTGTTCCTGGTGTTGTA+GGG 0.421839 2_2:+33582858 None:intergenic
GGAGGATCATCATCAGTCTA+TGG 0.429914 2_2:-33581797 Msa0248810:CDS
TTGTTGGAGTTGTTGTAGTT+GGG 0.434457 2_2:+33582621 None:intergenic
GTGGTGTCATACTGCTTGGT+GGG 0.437280 2_2:+33581846 None:intergenic
TGAAATCTTGCAAAATCTCC+TGG 0.440152 2_2:+33581587 None:intergenic
CAGGTGGTTGAGGAGATGTT+GGG 0.444649 2_2:+33582813 None:intergenic
AACCCTACAACACCAGGAAC+AGG 0.450399 2_2:-33582860 Msa0248810:CDS
GTTTGTCCAGTACTTGTGTC+AGG 0.458343 2_2:+33582794 None:intergenic
GCCTGTGTCTGGGCTTGGAG+GGG 0.461279 2_2:+33582772 None:intergenic
TCTATATTGTTTCAGGTCAT+GGG 0.461326 2_2:-33582057 Msa0248810:intron
GATTTGAGTATGGATTTGTT+GGG 0.463196 2_2:+33582741 None:intergenic
GAGTATGGATTTGTAGAAGC+TGG 0.464844 2_2:+33582686 None:intergenic
TCATATATGACATGTTCTAC+AGG 0.469415 2_2:-33582962 None:intergenic
GAGTATGGATTTGTTGAACT+TGG 0.470965 2_2:+33582656 None:intergenic
CTTGGTGGGGTCATAGTAGT+AGG 0.472356 2_2:+33581860 None:intergenic
GGTGGTGTCATACTGCTTGG+TGG 0.479946 2_2:+33581845 None:intergenic
GATCCTGTTCCTGGTGTTGT+GGG 0.480455 2_2:+33582584 None:intergenic
ACACCAGGAACAGGATCATC+TGG 0.480901 2_2:-33582578 Msa0248810:CDS
TATGGATTTGTTGAACTTGG+TGG 0.489297 2_2:+33582659 None:intergenic
GGTGTTGTAGGGTTCACAAT+AGG 0.490997 2_2:+33582869 None:intergenic
ACTCCCACAACACCAGGAAC+AGG 0.492125 2_2:-33582587 Msa0248810:CDS
GATGATCCTCCATCAGCATC+AGG 0.496560 2_2:+33581809 None:intergenic
GTACTTGTGTCAGGTGGTTG+AGG 0.502277 2_2:+33582803 None:intergenic
ACAGGATCATCTGGTGGTGG+TGG 0.506368 2_2:-33582569 Msa0248810:CDS
GTAGCTTTGGATTATGCTTG+TGG 0.506964 2_2:-33582482 Msa0248810:CDS
GACTGTGTGAATTCCAATCA+AGG 0.510660 2_2:+33581612 None:intergenic
TGATCCTGTTCCTGGTGTTG+TGG 0.511505 2_2:+33582583 None:intergenic
GAATATGGACTTGTAGAGGT+TGG 0.519554 2_2:+33582716 None:intergenic
CTAGGTGGAGTCATAGTAGT+TGG 0.526635 2_2:+33581884 None:intergenic
ATGGATTTGTTGAACTTGGT+GGG 0.527334 2_2:+33582660 None:intergenic
CTTGGTGGTGTCATACTGCT+TGG 0.530201 2_2:+33581842 None:intergenic
TCAGTCTATGGTTCACCACC+AGG 0.530618 2_2:-33581785 Msa0248810:CDS
ATGGATTTGTAGAAGCTGGT+GGG 0.535192 2_2:+33582690 None:intergenic
GCATCAGGCATTGTCATGCT+TGG 0.540422 2_2:+33581824 None:intergenic
CAACCACCTGACACAAGTAC+TGG 0.540527 2_2:-33582800 Msa0248810:CDS
TATGGACTTGTAGAGGTTGG+TGG 0.540851 2_2:+33582719 None:intergenic
GCACATGAAGTGTAGCATAG+AGG 0.541615 2_2:+33581702 None:intergenic
ACCCCTCCAAGCCCAGACAC+AGG 0.555256 2_2:-33582773 Msa0248810:CDS
TCAGGCATTGTCATGCTTGG+TGG 0.555903 2_2:+33581827 None:intergenic
GCTATTGATTGAATATGGAA+TGG 0.558429 2_2:-33581324 Msa0248810:three_prime_UTR
CATTGACATGTGTCAGACAC+TGG 0.559610 2_2:-33581655 Msa0248810:three_prime_UTR
TGTAGTGAGTAGTAGCAGAA+TGG 0.563778 2_2:+33581727 None:intergenic
GTGGAGTCATAGTAGTTGGT+GGG 0.565923 2_2:+33581888 None:intergenic
CCTACAAGTTGTGTATTTGG+AGG 0.565962 2_2:-33582347 Msa0248810:CDS
AATGTGACTCCCACAACACC+AGG 0.569381 2_2:-33582593 Msa0248810:CDS
AATACACAACTTGTAGGTGC+AGG 0.573202 2_2:+33582353 None:intergenic
GGTGGAGTCATAGTAGTTGG+TGG 0.575064 2_2:+33581887 None:intergenic
GTTGGTGGGCTTACAAATGT+TGG 0.600377 2_2:+33581902 None:intergenic
TGTCCAGTACTTGTGTCAGG+TGG 0.608903 2_2:+33582797 None:intergenic
GGGGTCATAGTAGTAGGACT+AGG 0.609330 2_2:+33581866 None:intergenic
ATGACAATGCCTGATGCTGA+TGG 0.613075 2_2:-33581818 Msa0248810:CDS
AAGTTGATGTAGCTATATTG+GGG 0.613710 2_2:+33581756 None:intergenic
GGTGGGCTTACAAATGTTGG+TGG 0.618577 2_2:+33581905 None:intergenic
GTTTGAATATGGACTTGTAG+AGG 0.619433 2_2:+33582712 None:intergenic
GGAACAGGATCATCTGGTGG+TGG 0.620332 2_2:-33582572 Msa0248810:CDS
ATTGTGAACCCTACAACACC+AGG 0.620914 2_2:-33582866 Msa0248810:CDS
ATACTATATTGATGAAAACA+TGG 0.624583 2_2:-33581366 Msa0248810:three_prime_UTR
CCTACAACACCAGGAACAGG+AGG 0.624591 2_2:-33582857 Msa0248810:CDS
TATGGATTTGTAGAAGCTGG+TGG 0.627475 2_2:+33582689 None:intergenic
TGGTGTCATACTGCTTGGTG+GGG 0.639890 2_2:+33581847 None:intergenic
ATATTGGGGCTTCCTCCTGG+TGG 0.641948 2_2:+33581770 None:intergenic
GTCATAGTAGTAGGACTAGG+TGG 0.650747 2_2:+33581869 None:intergenic
TGTGAATTCCAATCAAGGGT+CGG 0.656464 2_2:+33581617 None:intergenic
ACTGTGTGAATTCCAATCAA+GGG 0.659940 2_2:+33581613 None:intergenic
TAAATGGATACATTGATGCA+CGG 0.666819 2_2:+33581492 None:intergenic
ACAATGCCTGATGCTGATGG+AGG 0.696819 2_2:-33581815 Msa0248810:CDS
TCTCATCCTCCAAAACAACA+AGG 0.698612 2_2:-33582024 Msa0248810:intron
CCAGGAACAGGATCATCTGG+TGG 0.699045 2_2:-33582575 Msa0248810:CDS
GATTGTTCACAACTTCAACA+AGG 0.714222 2_2:-33582449 Msa0248810:CDS
GTCTATGGTTCACCACCAGG+AGG 0.724042 2_2:-33581782 Msa0248810:CDS
TGTTCACAACTTCAACAAGG+AGG 0.728282 2_2:-33582446 Msa0248810:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCATGATGAATTGATATTA+AGG + chr2_2:33582001-33582020 None:intergenic 20.0%
!!! AGTTTTATGCAATACTAATA+AGG + chr2_2:33582299-33582318 None:intergenic 20.0%
!! AGATTATCTATTTCAGATAA+AGG - chr2_2:33582866-33582885 Msa0248810:CDS 20.0%
!! GATTATCTATTTCAGATAAA+GGG - chr2_2:33582867-33582886 Msa0248810:CDS 20.0%
!! ATACTATATTGATGAAAACA+TGG - chr2_2:33582901-33582920 Msa0248810:CDS 20.0%
!! ATCATGATGAATTGATATTA+AGG + chr2_2:33582001-33582020 None:intergenic 20.0%
!!! AGTTTTATGCAATACTAATA+AGG + chr2_2:33582299-33582318 None:intergenic 20.0%
!! AGATTATCTATTTCAGATAA+AGG - chr2_2:33582866-33582885 Msa0248810:CDS 20.0%
!! GATTATCTATTTCAGATAAA+GGG - chr2_2:33582867-33582886 Msa0248810:CDS 20.0%
!! ATACTATATTGATGAAAACA+TGG - chr2_2:33582901-33582920 Msa0248810:CDS 20.0%
! TGAATCTTCTCTTAATCTTA+TGG + chr2_2:33581342-33581361 None:intergenic 25.0%
!! CATGATCTTTCAAAGTATTT+GGG + chr2_2:33581859-33581878 None:intergenic 25.0%
! TGTTGTATGAATATCTTACT+TGG + chr2_2:33581971-33581990 None:intergenic 25.0%
! AGATATTCATACAACATTGA+AGG - chr2_2:33581975-33581994 Msa0248810:intron 25.0%
! AATTCATCATGATCATTGAA+CGG - chr2_2:33582008-33582027 Msa0248810:intron 25.0%
! AACTTGATTTAGATCATGTT+TGG + chr2_2:33582175-33582194 None:intergenic 25.0%
!!! GTTGTTTTCTATATTGTTTC+AGG - chr2_2:33582203-33582222 Msa0248810:intron 25.0%
! TAAGGTGAATATGATATAAG+TGG + chr2_2:33582281-33582300 None:intergenic 25.0%
! AAGGACAATACATATATTAG+AGG + chr2_2:33582326-33582345 None:intergenic 25.0%
! ATAAATCTGCTAATGCTAAA+TGG + chr2_2:33582794-33582813 None:intergenic 25.0%
! TTTAGCATTAGCAGATTTAT+AGG - chr2_2:33582794-33582813 Msa0248810:CDS 25.0%
! TGAATCTTCTCTTAATCTTA+TGG + chr2_2:33581342-33581361 None:intergenic 25.0%
!! CATGATCTTTCAAAGTATTT+GGG + chr2_2:33581859-33581878 None:intergenic 25.0%
! TGTTGTATGAATATCTTACT+TGG + chr2_2:33581971-33581990 None:intergenic 25.0%
! AGATATTCATACAACATTGA+AGG - chr2_2:33581975-33581994 Msa0248810:intron 25.0%
! AATTCATCATGATCATTGAA+CGG - chr2_2:33582008-33582027 Msa0248810:intron 25.0%
! AACTTGATTTAGATCATGTT+TGG + chr2_2:33582175-33582194 None:intergenic 25.0%
!!! GTTGTTTTCTATATTGTTTC+AGG - chr2_2:33582203-33582222 Msa0248810:intron 25.0%
! TAAGGTGAATATGATATAAG+TGG + chr2_2:33582281-33582300 None:intergenic 25.0%
! AAGGACAATACATATATTAG+AGG + chr2_2:33582326-33582345 None:intergenic 25.0%
! ATAAATCTGCTAATGCTAAA+TGG + chr2_2:33582794-33582813 None:intergenic 25.0%
! TTTAGCATTAGCAGATTTAT+AGG - chr2_2:33582794-33582813 Msa0248810:CDS 25.0%
! GATTTGAGTATGGATTTGTT+GGG + chr2_2:33581529-33581548 None:intergenic 30.0%
! GCATGATCTTTCAAAGTATT+TGG + chr2_2:33581860-33581879 None:intergenic 30.0%
! TTCTATATTGTTTCAGGTCA+TGG - chr2_2:33582209-33582228 Msa0248810:intron 30.0%
! TCTATATTGTTTCAGGTCAT+GGG - chr2_2:33582210-33582229 Msa0248810:intron 30.0%
!!! AAATTCTCACCTTGTTGTTT+TGG + chr2_2:33582255-33582274 None:intergenic 30.0%
!! AAGTTGATGTAGCTATATTG+GGG + chr2_2:33582514-33582533 None:intergenic 30.0%
!! GAAGTTGATGTAGCTATATT+GGG + chr2_2:33582515-33582534 None:intergenic 30.0%
!! AGAAGTTGATGTAGCTATAT+TGG + chr2_2:33582516-33582535 None:intergenic 30.0%
CTTAGTTGCAAGAATTCATA+TGG + chr2_2:33582734-33582753 None:intergenic 30.0%
TAAATGGATACATTGATGCA+CGG + chr2_2:33582778-33582797 None:intergenic 30.0%
! GCATAGCTATTGATTGAATA+TGG - chr2_2:33582938-33582957 Msa0248810:CDS 30.0%
! GCTATTGATTGAATATGGAA+TGG - chr2_2:33582943-33582962 Msa0248810:CDS 30.0%
! GATTTGAGTATGGATTTGTT+GGG + chr2_2:33581529-33581548 None:intergenic 30.0%
! GCATGATCTTTCAAAGTATT+TGG + chr2_2:33581860-33581879 None:intergenic 30.0%
! TTCTATATTGTTTCAGGTCA+TGG - chr2_2:33582209-33582228 Msa0248810:intron 30.0%
! TCTATATTGTTTCAGGTCAT+GGG - chr2_2:33582210-33582229 Msa0248810:intron 30.0%
!!! AAATTCTCACCTTGTTGTTT+TGG + chr2_2:33582255-33582274 None:intergenic 30.0%
!! AAGTTGATGTAGCTATATTG+GGG + chr2_2:33582514-33582533 None:intergenic 30.0%
!! GAAGTTGATGTAGCTATATT+GGG + chr2_2:33582515-33582534 None:intergenic 30.0%
!! AGAAGTTGATGTAGCTATAT+TGG + chr2_2:33582516-33582535 None:intergenic 30.0%
CTTAGTTGCAAGAATTCATA+TGG + chr2_2:33582734-33582753 None:intergenic 30.0%
TAAATGGATACATTGATGCA+CGG + chr2_2:33582778-33582797 None:intergenic 30.0%
! GCATAGCTATTGATTGAATA+TGG - chr2_2:33582938-33582957 Msa0248810:CDS 30.0%
! GCTATTGATTGAATATGGAA+TGG - chr2_2:33582943-33582962 Msa0248810:CDS 30.0%
ATGACATGTTCTACAGGTTT+AGG - chr2_2:33581311-33581330 Msa0248810:three_prime_UTR 35.0%
GTTGGGTTTATAGTAGGATA+AGG + chr2_2:33581440-33581459 None:intergenic 35.0%
GGATTTGAGTATGGATTTGT+TGG + chr2_2:33581530-33581549 None:intergenic 35.0%
GTTTGAATATGGACTTGTAG+AGG + chr2_2:33581558-33581577 None:intergenic 35.0%
!! ATGGATTTGTTGAACTTGGT+GGG + chr2_2:33581610-33581629 None:intergenic 35.0%
!! TATGGATTTGTTGAACTTGG+TGG + chr2_2:33581611-33581630 None:intergenic 35.0%
!! GAGTATGGATTTGTTGAACT+TGG + chr2_2:33581614-33581633 None:intergenic 35.0%
TTGTTGGAGTTGTTGTAGTT+GGG + chr2_2:33581649-33581668 None:intergenic 35.0%
! GACTACTTTAAAGGTAGCTT+TGG - chr2_2:33581772-33581791 Msa0248810:CDS 35.0%
TTGGATTATGCTTGTGGATA+TGG - chr2_2:33581791-33581810 Msa0248810:CDS 35.0%
GATTGTTCACAACTTCAACA+AGG - chr2_2:33581818-33581837 Msa0248810:CDS 35.0%
ACATGTGTTAATGTTGTGTC+AGG + chr2_2:33582068-33582087 None:intergenic 35.0%
CATGTAAGTGCTTATGTACA+TGG + chr2_2:33582115-33582134 None:intergenic 35.0%
ACTGTGTGAATTCCAATCAA+GGG + chr2_2:33582657-33582676 None:intergenic 35.0%
TGAAATCTTGCAAAATCTCC+TGG + chr2_2:33582683-33582702 None:intergenic 35.0%
ATGACATGTTCTACAGGTTT+AGG - chr2_2:33581311-33581330 Msa0248810:three_prime_UTR 35.0%
GTTGGGTTTATAGTAGGATA+AGG + chr2_2:33581440-33581459 None:intergenic 35.0%
GGATTTGAGTATGGATTTGT+TGG + chr2_2:33581530-33581549 None:intergenic 35.0%
GTTTGAATATGGACTTGTAG+AGG + chr2_2:33581558-33581577 None:intergenic 35.0%
!! ATGGATTTGTTGAACTTGGT+GGG + chr2_2:33581610-33581629 None:intergenic 35.0%
!! TATGGATTTGTTGAACTTGG+TGG + chr2_2:33581611-33581630 None:intergenic 35.0%
!! GAGTATGGATTTGTTGAACT+TGG + chr2_2:33581614-33581633 None:intergenic 35.0%
TTGTTGGAGTTGTTGTAGTT+GGG + chr2_2:33581649-33581668 None:intergenic 35.0%
! GACTACTTTAAAGGTAGCTT+TGG - chr2_2:33581772-33581791 Msa0248810:CDS 35.0%
TTGGATTATGCTTGTGGATA+TGG - chr2_2:33581791-33581810 Msa0248810:CDS 35.0%
GATTGTTCACAACTTCAACA+AGG - chr2_2:33581818-33581837 Msa0248810:CDS 35.0%
ACATGTGTTAATGTTGTGTC+AGG + chr2_2:33582068-33582087 None:intergenic 35.0%
CATGTAAGTGCTTATGTACA+TGG + chr2_2:33582115-33582134 None:intergenic 35.0%
ACTGTGTGAATTCCAATCAA+GGG + chr2_2:33582657-33582676 None:intergenic 35.0%
TGAAATCTTGCAAAATCTCC+TGG + chr2_2:33582683-33582702 None:intergenic 35.0%
GGAGATGTTGGGTTTATAGT+AGG + chr2_2:33581446-33581465 None:intergenic 40.0%
GAATATGGACTTGTAGAGGT+TGG + chr2_2:33581554-33581573 None:intergenic 40.0%
ATGGATTTGTAGAAGCTGGT+GGG + chr2_2:33581580-33581599 None:intergenic 40.0%
TATGGATTTGTAGAAGCTGG+TGG + chr2_2:33581581-33581600 None:intergenic 40.0%
GAGTATGGATTTGTAGAAGC+TGG + chr2_2:33581584-33581603 None:intergenic 40.0%
! TTGTTGTAGTTGGGCTTGTT+GGG + chr2_2:33581640-33581659 None:intergenic 40.0%
CTTGTTGGAGTTGTTGTAGT+TGG + chr2_2:33581650-33581669 None:intergenic 40.0%
AGCTGCAGAGACTACTTTAA+AGG - chr2_2:33581763-33581782 Msa0248810:CDS 40.0%
!! GTAGCTTTGGATTATGCTTG+TGG - chr2_2:33581785-33581804 Msa0248810:CDS 40.0%
TGTTCACAACTTCAACAAGG+AGG - chr2_2:33581821-33581840 Msa0248810:CDS 40.0%
AATACACAACTTGTAGGTGC+AGG + chr2_2:33581917-33581936 None:intergenic 40.0%
GCACCTACAAGTTGTGTATT+TGG - chr2_2:33581917-33581936 Msa0248810:intron 40.0%
CCTCCAAATACACAACTTGT+AGG + chr2_2:33581923-33581942 None:intergenic 40.0%
CCTACAAGTTGTGTATTTGG+AGG - chr2_2:33581920-33581939 Msa0248810:intron 40.0%
!!! TTGTTTTGGAGGATGAGAAG+TGG + chr2_2:33582241-33582260 None:intergenic 40.0%
TCTCATCCTCCAAAACAACA+AGG - chr2_2:33582243-33582262 Msa0248810:intron 40.0%
!!! TTCTCACCTTGTTGTTTTGG+AGG + chr2_2:33582252-33582271 None:intergenic 40.0%
TGTAGTGAGTAGTAGCAGAA+TGG + chr2_2:33582543-33582562 None:intergenic 40.0%
ATATTACACCGACCCTTGAT+TGG - chr2_2:33582642-33582661 Msa0248810:CDS 40.0%
TGTGAATTCCAATCAAGGGT+CGG + chr2_2:33582653-33582672 None:intergenic 40.0%
GACTGTGTGAATTCCAATCA+AGG + chr2_2:33582658-33582677 None:intergenic 40.0%
GGAGATGTTGGGTTTATAGT+AGG + chr2_2:33581446-33581465 None:intergenic 40.0%
GAATATGGACTTGTAGAGGT+TGG + chr2_2:33581554-33581573 None:intergenic 40.0%
ATGGATTTGTAGAAGCTGGT+GGG + chr2_2:33581580-33581599 None:intergenic 40.0%
TATGGATTTGTAGAAGCTGG+TGG + chr2_2:33581581-33581600 None:intergenic 40.0%
GAGTATGGATTTGTAGAAGC+TGG + chr2_2:33581584-33581603 None:intergenic 40.0%
! TTGTTGTAGTTGGGCTTGTT+GGG + chr2_2:33581640-33581659 None:intergenic 40.0%
CTTGTTGGAGTTGTTGTAGT+TGG + chr2_2:33581650-33581669 None:intergenic 40.0%
AGCTGCAGAGACTACTTTAA+AGG - chr2_2:33581763-33581782 Msa0248810:CDS 40.0%
!! GTAGCTTTGGATTATGCTTG+TGG - chr2_2:33581785-33581804 Msa0248810:CDS 40.0%
TGTTCACAACTTCAACAAGG+AGG - chr2_2:33581821-33581840 Msa0248810:CDS 40.0%
AATACACAACTTGTAGGTGC+AGG + chr2_2:33581917-33581936 None:intergenic 40.0%
GCACCTACAAGTTGTGTATT+TGG - chr2_2:33581917-33581936 Msa0248810:intron 40.0%
CCTCCAAATACACAACTTGT+AGG + chr2_2:33581923-33581942 None:intergenic 40.0%
CCTACAAGTTGTGTATTTGG+AGG - chr2_2:33581920-33581939 Msa0248810:intron 40.0%
!!! TTGTTTTGGAGGATGAGAAG+TGG + chr2_2:33582241-33582260 None:intergenic 40.0%
TCTCATCCTCCAAAACAACA+AGG - chr2_2:33582243-33582262 Msa0248810:intron 40.0%
!!! TTCTCACCTTGTTGTTTTGG+AGG + chr2_2:33582252-33582271 None:intergenic 40.0%
TGTAGTGAGTAGTAGCAGAA+TGG + chr2_2:33582543-33582562 None:intergenic 40.0%
ATATTACACCGACCCTTGAT+TGG - chr2_2:33582642-33582661 Msa0248810:CDS 40.0%
TGTGAATTCCAATCAAGGGT+CGG + chr2_2:33582653-33582672 None:intergenic 40.0%
GACTGTGTGAATTCCAATCA+AGG + chr2_2:33582658-33582677 None:intergenic 40.0%
! GGTGTTGTAGGGTTCACAAT+AGG + chr2_2:33581401-33581420 None:intergenic 45.0%
ATTGTGAACCCTACAACACC+AGG - chr2_2:33581401-33581420 Msa0248810:three_prime_UTR 45.0%
TAAGGATTTCCTCCTGTTCC+TGG + chr2_2:33581422-33581441 None:intergenic 45.0%
GTTTGTCCAGTACTTGTGTC+AGG + chr2_2:33581476-33581495 None:intergenic 45.0%
!! GAGGTTGGTGGATTTGAGTA+TGG + chr2_2:33581539-33581558 None:intergenic 45.0%
TATGGACTTGTAGAGGTTGG+TGG + chr2_2:33581551-33581570 None:intergenic 45.0%
!! GAAGCTGGTGGGTTTGAATA+TGG + chr2_2:33581569-33581588 None:intergenic 45.0%
!! GAACTTGGTGGGTTTGAGTA+TGG + chr2_2:33581599-33581618 None:intergenic 45.0%
GTTGTTGTAGTTGGGCTTGT+TGG + chr2_2:33581641-33581660 None:intergenic 45.0%
GTGTTGTGGGAGTCACATTT+GGG + chr2_2:33581673-33581692 None:intergenic 45.0%
GATCATTGAACGGTAGTGTC+TGG - chr2_2:33582018-33582037 Msa0248810:intron 45.0%
GGTCAGACACTGATACATGT+CGG + chr2_2:33582047-33582066 None:intergenic 45.0%
TGGACTCATGCTGTAATGCA+AGG + chr2_2:33582345-33582364 None:intergenic 45.0%
!! GTTGGTGGGCTTACAAATGT+TGG + chr2_2:33582368-33582387 None:intergenic 45.0%
GTGGAGTCATAGTAGTTGGT+GGG + chr2_2:33582382-33582401 None:intergenic 45.0%
CTAGGTGGAGTCATAGTAGT+TGG + chr2_2:33582386-33582405 None:intergenic 45.0%
GTCATAGTAGTAGGACTAGG+TGG + chr2_2:33582401-33582420 None:intergenic 45.0%
ATGACAATGCCTGATGCTGA+TGG - chr2_2:33582449-33582468 Msa0248810:CDS 45.0%
GGAGGATCATCATCAGTCTA+TGG - chr2_2:33582470-33582489 Msa0248810:CDS 45.0%
GCACATGAAGTGTAGCATAG+AGG + chr2_2:33582568-33582587 None:intergenic 45.0%
CATTGACATGTGTCAGACAC+TGG - chr2_2:33582612-33582631 Msa0248810:CDS 45.0%
TGGAATTCACACAGTCTTCC+AGG - chr2_2:33582662-33582681 Msa0248810:CDS 45.0%
! GGTGTTGTAGGGTTCACAAT+AGG + chr2_2:33581401-33581420 None:intergenic 45.0%
ATTGTGAACCCTACAACACC+AGG - chr2_2:33581401-33581420 Msa0248810:three_prime_UTR 45.0%
TAAGGATTTCCTCCTGTTCC+TGG + chr2_2:33581422-33581441 None:intergenic 45.0%
GTTTGTCCAGTACTTGTGTC+AGG + chr2_2:33581476-33581495 None:intergenic 45.0%
!! GAGGTTGGTGGATTTGAGTA+TGG + chr2_2:33581539-33581558 None:intergenic 45.0%
TATGGACTTGTAGAGGTTGG+TGG + chr2_2:33581551-33581570 None:intergenic 45.0%
!! GAAGCTGGTGGGTTTGAATA+TGG + chr2_2:33581569-33581588 None:intergenic 45.0%
!! GAACTTGGTGGGTTTGAGTA+TGG + chr2_2:33581599-33581618 None:intergenic 45.0%
GTTGTTGTAGTTGGGCTTGT+TGG + chr2_2:33581641-33581660 None:intergenic 45.0%
GTGTTGTGGGAGTCACATTT+GGG + chr2_2:33581673-33581692 None:intergenic 45.0%
GATCATTGAACGGTAGTGTC+TGG - chr2_2:33582018-33582037 Msa0248810:intron 45.0%
GGTCAGACACTGATACATGT+CGG + chr2_2:33582047-33582066 None:intergenic 45.0%
TGGACTCATGCTGTAATGCA+AGG + chr2_2:33582345-33582364 None:intergenic 45.0%
!! GTTGGTGGGCTTACAAATGT+TGG + chr2_2:33582368-33582387 None:intergenic 45.0%
GTGGAGTCATAGTAGTTGGT+GGG + chr2_2:33582382-33582401 None:intergenic 45.0%
CTAGGTGGAGTCATAGTAGT+TGG + chr2_2:33582386-33582405 None:intergenic 45.0%
GTCATAGTAGTAGGACTAGG+TGG + chr2_2:33582401-33582420 None:intergenic 45.0%
ATGACAATGCCTGATGCTGA+TGG - chr2_2:33582449-33582468 Msa0248810:CDS 45.0%
GGAGGATCATCATCAGTCTA+TGG - chr2_2:33582470-33582489 Msa0248810:CDS 45.0%
GCACATGAAGTGTAGCATAG+AGG + chr2_2:33582568-33582587 None:intergenic 45.0%
CATTGACATGTGTCAGACAC+TGG - chr2_2:33582612-33582631 Msa0248810:CDS 45.0%
TGGAATTCACACAGTCTTCC+AGG - chr2_2:33582662-33582681 Msa0248810:CDS 45.0%
AACCCTACAACACCAGGAAC+AGG - chr2_2:33581407-33581426 Msa0248810:three_prime_UTR 50.0%
!! CTCCTGTTCCTGGTGTTGTA+GGG + chr2_2:33581412-33581431 None:intergenic 50.0%
CAGGTGGTTGAGGAGATGTT+GGG + chr2_2:33581457-33581476 None:intergenic 50.0%
TCAGGTGGTTGAGGAGATGT+TGG + chr2_2:33581458-33581477 None:intergenic 50.0%
GTACTTGTGTCAGGTGGTTG+AGG + chr2_2:33581467-33581486 None:intergenic 50.0%
CAACCACCTGACACAAGTAC+TGG - chr2_2:33581467-33581486 Msa0248810:three_prime_UTR 50.0%
TGTCCAGTACTTGTGTCAGG+TGG + chr2_2:33581473-33581492 None:intergenic 50.0%
!! GGGCTTGTTGGGTTTGAGTA+TGG + chr2_2:33581629-33581648 None:intergenic 50.0%
GGAGTCACATTTGGGCTTGT+TGG + chr2_2:33581665-33581684 None:intergenic 50.0%
! GGTGTTGTGGGAGTCACATT+TGG + chr2_2:33581674-33581693 None:intergenic 50.0%
AATGTGACTCCCACAACACC+AGG - chr2_2:33581674-33581693 Msa0248810:exon 50.0%
!! GATCCTGTTCCTGGTGTTGT+GGG + chr2_2:33581686-33581705 None:intergenic 50.0%
! TGATCCTGTTCCTGGTGTTG+TGG + chr2_2:33581687-33581706 None:intergenic 50.0%
ACACCAGGAACAGGATCATC+TGG - chr2_2:33581689-33581708 Msa0248810:CDS 50.0%
! GGTGGGCTTACAAATGTTGG+TGG + chr2_2:33582365-33582384 None:intergenic 50.0%
GGTGGAGTCATAGTAGTTGG+TGG + chr2_2:33582383-33582402 None:intergenic 50.0%
GGGGTCATAGTAGTAGGACT+AGG + chr2_2:33582404-33582423 None:intergenic 50.0%
CTTGGTGGGGTCATAGTAGT+AGG + chr2_2:33582410-33582429 None:intergenic 50.0%
!! TGGTGTCATACTGCTTGGTG+GGG + chr2_2:33582423-33582442 None:intergenic 50.0%
!! GTGGTGTCATACTGCTTGGT+GGG + chr2_2:33582424-33582443 None:intergenic 50.0%
!! CTTGGTGGTGTCATACTGCT+TGG + chr2_2:33582428-33582447 None:intergenic 50.0%
! TCAGGCATTGTCATGCTTGG+TGG + chr2_2:33582443-33582462 None:intergenic 50.0%
! GCATCAGGCATTGTCATGCT+TGG + chr2_2:33582446-33582465 None:intergenic 50.0%
ACAATGCCTGATGCTGATGG+AGG - chr2_2:33582452-33582471 Msa0248810:CDS 50.0%
GATGATCCTCCATCAGCATC+AGG + chr2_2:33582461-33582480 None:intergenic 50.0%
TCAGTCTATGGTTCACCACC+AGG - chr2_2:33582482-33582501 Msa0248810:CDS 50.0%
AACCCTACAACACCAGGAAC+AGG - chr2_2:33581407-33581426 Msa0248810:three_prime_UTR 50.0%
!! CTCCTGTTCCTGGTGTTGTA+GGG + chr2_2:33581412-33581431 None:intergenic 50.0%
CAGGTGGTTGAGGAGATGTT+GGG + chr2_2:33581457-33581476 None:intergenic 50.0%
TCAGGTGGTTGAGGAGATGT+TGG + chr2_2:33581458-33581477 None:intergenic 50.0%
GTACTTGTGTCAGGTGGTTG+AGG + chr2_2:33581467-33581486 None:intergenic 50.0%
CAACCACCTGACACAAGTAC+TGG - chr2_2:33581467-33581486 Msa0248810:three_prime_UTR 50.0%
TGTCCAGTACTTGTGTCAGG+TGG + chr2_2:33581473-33581492 None:intergenic 50.0%
!! GGGCTTGTTGGGTTTGAGTA+TGG + chr2_2:33581629-33581648 None:intergenic 50.0%
GGAGTCACATTTGGGCTTGT+TGG + chr2_2:33581665-33581684 None:intergenic 50.0%
! GGTGTTGTGGGAGTCACATT+TGG + chr2_2:33581674-33581693 None:intergenic 50.0%
AATGTGACTCCCACAACACC+AGG - chr2_2:33581674-33581693 Msa0248810:exon 50.0%
!! GATCCTGTTCCTGGTGTTGT+GGG + chr2_2:33581686-33581705 None:intergenic 50.0%
! TGATCCTGTTCCTGGTGTTG+TGG + chr2_2:33581687-33581706 None:intergenic 50.0%
ACACCAGGAACAGGATCATC+TGG - chr2_2:33581689-33581708 Msa0248810:CDS 50.0%
! GGTGGGCTTACAAATGTTGG+TGG + chr2_2:33582365-33582384 None:intergenic 50.0%
GGTGGAGTCATAGTAGTTGG+TGG + chr2_2:33582383-33582402 None:intergenic 50.0%
GGGGTCATAGTAGTAGGACT+AGG + chr2_2:33582404-33582423 None:intergenic 50.0%
CTTGGTGGGGTCATAGTAGT+AGG + chr2_2:33582410-33582429 None:intergenic 50.0%
!! TGGTGTCATACTGCTTGGTG+GGG + chr2_2:33582423-33582442 None:intergenic 50.0%
!! GTGGTGTCATACTGCTTGGT+GGG + chr2_2:33582424-33582443 None:intergenic 50.0%
!! CTTGGTGGTGTCATACTGCT+TGG + chr2_2:33582428-33582447 None:intergenic 50.0%
! TCAGGCATTGTCATGCTTGG+TGG + chr2_2:33582443-33582462 None:intergenic 50.0%
! GCATCAGGCATTGTCATGCT+TGG + chr2_2:33582446-33582465 None:intergenic 50.0%
ACAATGCCTGATGCTGATGG+AGG - chr2_2:33582452-33582471 Msa0248810:CDS 50.0%
GATGATCCTCCATCAGCATC+AGG + chr2_2:33582461-33582480 None:intergenic 50.0%
TCAGTCTATGGTTCACCACC+AGG - chr2_2:33582482-33582501 Msa0248810:CDS 50.0%
!! CCTCCTGTTCCTGGTGTTGT+AGG + chr2_2:33581413-33581432 None:intergenic 55.0%
CCTACAACACCAGGAACAGG+AGG - chr2_2:33581410-33581429 Msa0248810:three_prime_UTR 55.0%
!! GGCTTGTTGTGCCTGTGTCT+GGG + chr2_2:33581508-33581527 None:intergenic 55.0%
ACTCCCACAACACCAGGAAC+AGG - chr2_2:33581680-33581699 Msa0248810:exon 55.0%
CCACCAGATGATCCTGTTCC+TGG + chr2_2:33581695-33581714 None:intergenic 55.0%
CCAGGAACAGGATCATCTGG+TGG - chr2_2:33581692-33581711 Msa0248810:CDS 55.0%
GGAACAGGATCATCTGGTGG+TGG - chr2_2:33581695-33581714 Msa0248810:CDS 55.0%
!! ACAGGATCATCTGGTGGTGG+TGG - chr2_2:33581698-33581717 Msa0248810:CDS 55.0%
!! GGTGGTGTCATACTGCTTGG+TGG + chr2_2:33582425-33582444 None:intergenic 55.0%
GTCTATGGTTCACCACCAGG+AGG - chr2_2:33582485-33582504 Msa0248810:CDS 55.0%
ATATTGGGGCTTCCTCCTGG+TGG + chr2_2:33582500-33582519 None:intergenic 55.0%
GCTATATTGGGGCTTCCTCC+TGG + chr2_2:33582503-33582522 None:intergenic 55.0%
!! CCTCCTGTTCCTGGTGTTGT+AGG + chr2_2:33581413-33581432 None:intergenic 55.0%
CCTACAACACCAGGAACAGG+AGG - chr2_2:33581410-33581429 Msa0248810:three_prime_UTR 55.0%
!! GGCTTGTTGTGCCTGTGTCT+GGG + chr2_2:33581508-33581527 None:intergenic 55.0%
ACTCCCACAACACCAGGAAC+AGG - chr2_2:33581680-33581699 Msa0248810:exon 55.0%
CCACCAGATGATCCTGTTCC+TGG + chr2_2:33581695-33581714 None:intergenic 55.0%
CCAGGAACAGGATCATCTGG+TGG - chr2_2:33581692-33581711 Msa0248810:CDS 55.0%
GGAACAGGATCATCTGGTGG+TGG - chr2_2:33581695-33581714 Msa0248810:CDS 55.0%
!! ACAGGATCATCTGGTGGTGG+TGG - chr2_2:33581698-33581717 Msa0248810:CDS 55.0%
!! GGTGGTGTCATACTGCTTGG+TGG + chr2_2:33582425-33582444 None:intergenic 55.0%
GTCTATGGTTCACCACCAGG+AGG - chr2_2:33582485-33582504 Msa0248810:CDS 55.0%
ATATTGGGGCTTCCTCCTGG+TGG + chr2_2:33582500-33582519 None:intergenic 55.0%
GCTATATTGGGGCTTCCTCC+TGG + chr2_2:33582503-33582522 None:intergenic 55.0%
TGCCTGTGTCTGGGCTTGGA+GGG + chr2_2:33581499-33581518 None:intergenic 60.0%
GTTGTGCCTGTGTCTGGGCT+TGG + chr2_2:33581503-33581522 None:intergenic 60.0%
!! GGGCTTGTTGTGCCTGTGTC+TGG + chr2_2:33581509-33581528 None:intergenic 60.0%
!! GGATCATCTGGTGGTGGTGG+TGG - chr2_2:33581701-33581720 Msa0248810:CDS 60.0%
!! TCATCTGGTGGTGGTGGTGG+TGG - chr2_2:33581704-33581723 Msa0248810:CDS 60.0%
TGCCTGTGTCTGGGCTTGGA+GGG + chr2_2:33581499-33581518 None:intergenic 60.0%
GTTGTGCCTGTGTCTGGGCT+TGG + chr2_2:33581503-33581522 None:intergenic 60.0%
!! GGGCTTGTTGTGCCTGTGTC+TGG + chr2_2:33581509-33581528 None:intergenic 60.0%
!! GGATCATCTGGTGGTGGTGG+TGG - chr2_2:33581701-33581720 Msa0248810:CDS 60.0%
!! TCATCTGGTGGTGGTGGTGG+TGG - chr2_2:33581704-33581723 Msa0248810:CDS 60.0%
ACCCCTCCAAGCCCAGACAC+AGG - chr2_2:33581494-33581513 Msa0248810:three_prime_UTR 65.0%
GCCTGTGTCTGGGCTTGGAG+GGG + chr2_2:33581498-33581517 None:intergenic 65.0%
GTGCCTGTGTCTGGGCTTGG+AGG + chr2_2:33581500-33581519 None:intergenic 65.0%
!! TCTGGTGGTGGTGGTGGTGG+TGG - chr2_2:33581707-33581726 Msa0248810:CDS 65.0%
!! GTGGTGGTGGTGGTGGTCAG+TGG - chr2_2:33581723-33581742 Msa0248810:CDS 65.0%
ACCCCTCCAAGCCCAGACAC+AGG - chr2_2:33581494-33581513 Msa0248810:three_prime_UTR 65.0%
GCCTGTGTCTGGGCTTGGAG+GGG + chr2_2:33581498-33581517 None:intergenic 65.0%
GTGCCTGTGTCTGGGCTTGG+AGG + chr2_2:33581500-33581519 None:intergenic 65.0%
!! TCTGGTGGTGGTGGTGGTGG+TGG - chr2_2:33581707-33581726 Msa0248810:CDS 65.0%
!! GTGGTGGTGGTGGTGGTCAG+TGG - chr2_2:33581723-33581742 Msa0248810:CDS 65.0%
!! GGTGGTGGTGGTGGTGGTGG+TGG - chr2_2:33581710-33581729 Msa0248810:CDS 70.0%
!! GGTGGTGGTGGTGGTGGTGG+TGG - chr2_2:33581710-33581729 Msa0248810:CDS 70.0%
!! GGTGGTGGTGGTGGTGGTGG+TGG - chr2_2:33581710-33581729 Msa0248810:CDS 70.0%
!! GGTGGTGGTGGTGGTGGTGG+TGG - chr2_2:33581710-33581729 Msa0248810:CDS 70.0%
!! GGTGGTGGTGGTGGTGGTGG+TGG - chr2_2:33581710-33581729 Msa0248810:CDS 70.0%
!! GGTGGTGGTGGTGGTGGTGG+TGG - chr2_2:33581710-33581729 Msa0248810:CDS 70.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 33581311 33582978 33581311 ID=Msa0248810;Name=Msa0248810
chr2_2 mRNA 33581311 33582978 33581311 ID=Msa0248810-mRNA-1;Parent=Msa0248810;Name=Msa0248810-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|1|0.66|1|1|1|3|377|315
chr2_2 exon 33581311 33581934 33581311 ID=Msa0248810-mRNA-1:exon:11245;Parent=Msa0248810-mRNA-1
chr2_2 exon 33582025 33582064 33582025 ID=Msa0248810-mRNA-1:exon:11244;Parent=Msa0248810-mRNA-1
chr2_2 exon 33582318 33582978 33582318 ID=Msa0248810-mRNA-1:exon:11243;Parent=Msa0248810-mRNA-1
chr2_2 CDS 33582318 33582978 33582318 ID=Msa0248810-mRNA-1:cds;Parent=Msa0248810-mRNA-1
chr2_2 CDS 33582025 33582064 33582025 ID=Msa0248810-mRNA-1:cds;Parent=Msa0248810-mRNA-1
chr2_2 CDS 33581688 33581934 33581688 ID=Msa0248810-mRNA-1:cds;Parent=Msa0248810-mRNA-1
chr2_2 three_prime_UTR 33581311 33581687 33581311 ID=Msa0248810-mRNA-1:three_prime_utr;Parent=Msa0248810-mRNA-1
Gene Sequence

>Msa0248810

ATGACATGTTCTACAGGTTTAGGTACAGCCATAAGATTAAGAGAAGATTCATCATATGAATTTAGTACAACTCAATCAGATTCAATTCCTATTGTGAACCCTACAACACCAGGAACAGGAGGAAATCCTTATCCTACTATAAACCCAACATCTCCTCAACCACCTGACACAAGTACTGGACAAACCCCTCCAAGCCCAGACACAGGCACAACAAGCCCAACAAATCCATACTCAAATCCACCAACCTCTACAAGTCCATATTCAAACCCACCAGCTTCTACAAATCCATACTCAAACCCACCAAGTTCAACAAATCCATACTCAAACCCAACAAGCCCAACTACAACAACTCCAACAAGCCCAAATGTGACTCCCACAACACCAGGAACAGGATCATCTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTCAGTGGTGTGTTGCAAGTGAATCAGCTGCAGAGACTACTTTAAAGGTAGCTTTGGATTATGCTTGTGGATATGGTGCAGATTGTTCACAACTTCAACAAGGAGGAGCTTGTTATGACCCAAATACTTTGAAAGATCATGCTTCTTATGCATTCAATGATTACTATCAAAAGAATCCTGCACCTACAAGTTGTGTATTTGGAGGAGTAGCATCACTCACAAGCAAAGATCCAAGTCATGGGAATTGCCACTTCTCATCCTCCAAAACAACAAGCATGAGTCCACCAACATTTGTAAGCCCACCAACTACTATGACTCCACCTAGTCCTACTACTATGACCCCACCAAGCAGTATGACACCACCAAGCATGACAATGCCTGATGCTGATGGAGGATCATCATCAGTCTATGGTTCACCACCAGGAGGAAGCCCCAATATAGCTACATCAACTTCTTATTCCATTCTGCTACTACTCACTACAAGCCTCTATGCTACACTTCATGTGCAAAATTACGTCTAG
Protein sequence

>Msa0248810

MTCSTGLGTAIRLREDSSYEFSTTQSDSIPIVNPTTPGTGGNPYPTINPTSPQPPDTSTGQTPPSPDTGTTSPTNPYSNPPTSTSPYSNPPASTNPYSNPPSSTNPYSNPTSPTTTTPTSPNVTPTTPGTGSSGGGGGGGGGGQWCVASESAAETTLKVALDYACGYGADCSQLQQGGACYDPNTLKDHASYAFNDYYQKNPAPTSCVFGGVASLTSKDPSHGNCHFSSSKTTSMSPPTFVSPPTTMTPPSPTTMTPPSSMTPPSMTMPDADGGSSSVYGSPPGGSPNIATSTSYSILLLLTTSLYATLHVQNYV*