AlfalfaGEDB | Msa0325720

M. sativa cultivar zhongmu-4 / Msa0325720

KEGG: K03006
GO: -
PFAM: PF07983.16

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0325720	XP_003595584.1	94.225	329	17	2	1	328	1	328	7.23e-145	422

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0325720	sp\|O65399\|E131_ARATH	49.000	100	49	2	158	255	381	480	3.68e-21	97.4

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003740	Msa0325720	0.815285	9.997684e-52	-8.615850e-47
Msa0150590	Msa0325720	0.801684	8.017514e-49	-8.615850e-47
Msa0248810	Msa0325720	0.897665	1.127538e-76	-8.615850e-47
Msa0325720	Msa0427820	0.844050	9.610842e-59	-8.615850e-47
Msa0325720	Msa0474060	0.847511	1.104505e-59	-8.615850e-47
Msa0325720	Msa0518680	0.838312	3.098337e-57	-8.615850e-47
Msa0325720	Msa0741970	0.800322	1.521653e-48	-8.615850e-47
Msa0325720	Msa0862170	0.810780	9.722380e-51	-8.615850e-47
Msa0325720	Msa1060340	0.805343	1.397226e-49	-8.615850e-47
Msa0325720	Msa1267050	0.800234	1.585806e-48	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0325720	MtrunA17_Chr2g0304061	94.225	329	17	2	1	328	1	328	6.65e-149	422
Msa0325720	MtrunA17_Chr3g0096451	49.660	147	60	4	95	240	62	195	1.66e-34	127
Msa0325720	MtrunA17_Chr5g0443331	50.000	144	47	4	109	247	75	198	3.72e-33	123
Msa0325720	MtrunA17_Chr1g0193131	45.545	202	79	5	56	236	307	498	1.93e-31	124
Msa0325720	MtrunA17_Chr7g0271411	46.524	187	82	6	56	240	73	243	2.29e-30	117
Msa0325720	MtrunA17_Chr8g0378361	48.031	127	61	2	151	276	23	145	2.35e-29	111
Msa0325720	MtrunA17_Chr8g0381081	55.556	90	38	2	155	242	54	143	1.78e-26	103
Msa0325720	MtrunA17_Chr8g0380231	60.000	80	28	2	167	245	26	102	7.84e-26	99.4
Msa0325720	MtrunA17_Chr5g0447651	55.814	86	36	2	158	241	30	115	7.50e-25	97.1
Msa0325720	MtrunA17_Chr4g0075031	54.348	92	40	2	158	247	382	473	1.11e-24	104

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0325720	AT2G30933.1	58.947	95	38	1	154	247	78	172	5.32e-34	125
Msa0325720	AT1G09460.1	59.140	93	37	1	155	247	134	225	3.33e-30	117
Msa0325720	AT2G30933.2	60.976	82	31	1	154	234	78	159	7.69e-30	112
Msa0325720	AT2G30933.3	60.976	82	31	1	154	234	78	159	7.69e-30	112
Msa0325720	AT5G35740.1	52.809	89	37	4	157	241	29	116	2.23e-24	96.3
Msa0325720	AT4G13600.1	43.411	129	60	3	155	270	58	186	2.91e-24	99.4
Msa0325720	AT4G05430.1	47.312	93	47	2	155	245	20	112	3.15e-23	94.0
Msa0325720	AT4G05430.3	47.312	93	47	2	155	245	45	137	3.49e-23	94.7
Msa0325720	AT4G26830.1	41.406	128	64	4	115	240	333	451	2.85e-22	97.4
Msa0325720	AT4G26830.2	41.406	128	64	4	115	240	337	455	3.18e-22	97.1

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Find 128 sgRNAs with CRISPR-Local

Find 181 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATTCTCACCTTGTTGTTT+TGG	0.240571	2_4:+33586542	None:intergenic
AAGAAATGGGAGCTAGAAGT+TGG	0.544857	2_4:-33588499	Msa0325720:exon
AAGATTAAATTCTCCATCAC+AGG	0.523019	2_4:-33588626	Msa0325720:five_prime_UTR
AAGTTGATGTAGCTATATTG+GGG	0.613710	2_4:+33586283	None:intergenic
AATACACAACTTGTAGGTGC+AGG	0.573202	2_4:+33586880	None:intergenic
AATCCTACAACACCAGGAAC+AGG	0.478945	2_4:-33587384	Msa0325720:CDS
AATGTGACTCCCACAACACC+AGG	0.569381	2_4:-33587117	Msa0325720:CDS
ACAATGCCTGATGCTGATGG+AGG	0.696819	2_4:-33586342	Msa0325720:CDS
ACACCAGGAACAGGATCATC+TGG	0.480901	2_4:-33587102	Msa0325720:CDS
ACAGGATCATCTGGTGGTGG+TGG	0.506368	2_4:-33587093	Msa0325720:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGATTAAATTCTCCATCAC+AGG	-	chr2_4:33585844-33585863	Msa0325720:three_prime_UTR	30.0%
GTTTATGAGCAATGTTTAAG+AGG	+	chr2_4:33585910-33585929	None:intergenic	30.0%
ACATTGCTCATAAACTTGAA+AGG	-	chr2_4:33585915-33585934	Msa0325720:three_prime_UTR	30.0%
GACATAGCAAAAATAAGACA+AGG	+	chr2_4:33585999-33586018	None:intergenic	30.0%
AATAGTTGTGTTGTGTGATA+AGG	-	chr2_4:33586129-33586148	Msa0325720:three_prime_UTR	30.0%
TAGAAAAACAAGCATGTACA+TGG	+	chr2_4:33586543-33586562	None:intergenic	30.0%
TGACAACATTTGGATTCATT+AGG	-	chr2_4:33586582-33586601	Msa0325720:intron	30.0%
CTTAGTTGCAAGAATTCATA+TGG	+	chr2_4:33588410-33588429	None:intergenic	30.0%
TAAATGGATACATTGATGCA+AGG	+	chr2_4:33588454-33588473	None:intergenic	30.0%
TAAAATCACACCTGAACAGA+GGG	+	chr2_4:33586025-33586044	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	33585840	33588652	33585840	ID=Msa0325720;Name=Msa0325720
chr2_4	mRNA	33585840	33588652	33585840	ID=Msa0325720-mRNA-1;Parent=Msa0325720;Name=Msa0325720-mRNA-1;_AED=0.03;_eAED=0.03;_QI=136\|1\|0.75\|1\|1\|1\|4\|375\|328
chr2_4	exon	33585840	33586461	33585840	ID=Msa0325720-mRNA-1:exon:10786;Parent=Msa0325720-mRNA-1
chr2_4	exon	33586552	33586591	33586552	ID=Msa0325720-mRNA-1:exon:10785;Parent=Msa0325720-mRNA-1
chr2_4	exon	33586845	33587486	33586845	ID=Msa0325720-mRNA-1:exon:10784;Parent=Msa0325720-mRNA-1
chr2_4	exon	33588459	33588652	33588459	ID=Msa0325720-mRNA-1:exon:10783;Parent=Msa0325720-mRNA-1
chr2_4	five_prime_UTR	33588517	33588652	33588517	ID=Msa0325720-mRNA-1:five_prime_utr;Parent=Msa0325720-mRNA-1
chr2_4	CDS	33588459	33588516	33588459	ID=Msa0325720-mRNA-1:cds;Parent=Msa0325720-mRNA-1
chr2_4	CDS	33586845	33587486	33586845	ID=Msa0325720-mRNA-1:cds;Parent=Msa0325720-mRNA-1
chr2_4	CDS	33586552	33586591	33586552	ID=Msa0325720-mRNA-1:cds;Parent=Msa0325720-mRNA-1
chr2_4	CDS	33586215	33586461	33586215	ID=Msa0325720-mRNA-1:cds;Parent=Msa0325720-mRNA-1
chr2_4	three_prime_UTR	33585840	33586214	33585840	ID=Msa0325720-mRNA-1:three_prime_utr;Parent=Msa0325720-mRNA-1

Gene Sequence

>Msa0325720

ATGGGAGCTAGAAGTTGGCACCTTGTCTTATTTTTGCTATGTCTTTCCCTCTGTTCAGGTTTAGGTACAGCCATAAGATTAAGAGAAGATTCATCATATGAATTTAGTACAACTCAATCAGATTCAATTCCTATTGTGAATCCTACAACACCAGGAACAGGAGGAAATCCTTATCCTACTATAAACCCAACATCTCCTCAACCACCTGACACAAGTACTGGACAAACCCCACCAAGCCCAAACACAGGCACAACAAGCCCAACAAATCCATACTCAAACCCACCAACCTCTACAAGTCCCTATTCAAACCCACCAGCTTCTACAAATCCATACTCAAACCCACCAAGTTCAACAAATCCATACTCAAACCCAACAAGCCCAACTACAACAACTCCAACAAGCCCAAATGTGACTCCCACAACACCAGGAACAGGATCATCTGGTGGTGGTGGTGGCGGCGGTGGTGGTCAGTGGTGTGTTGCAAGTGAATCAGCTGCAGAGACTACTTTAAAGGTAGCTTTGGATTATGCTTGTGGATATGGTGCAGATTGTTCACAACTTCAACAAGGAGGAGCTTGTTATGACCCAAATACTTTGAAAGATCATGCTTCTTATGCATTCAATGATTACTATCAAAAGAATCCTGCACCTACAAGTTGTGTATTTGGAGGAGTAGCATCACTCACAAGCAAAGATCCAAGTCATGGGAATTGCCACTTCTCATCCTCCAAAACAACAAGCATGAGTCCACCAACATTTGTAAGCCCACCAACTACTATGACTCCACCTAGTCCTACTACTATGACCCCACCAAGCAGTATGACACCACCAAGCATGACAATGCCTGATGCTGATGGAGGATCATCATCAGTCTATGGTTCACCACCAGGAGGAAGCCCCAATATAGCTACATCAACTTCTTATTCCATTCTGCTACTACTCACTACAAGCCTCTATGCTACACTTCATGTGCAAAATTATGTCTAG

Protein sequence

>Msa0325720

MGARSWHLVLFLLCLSLCSGLGTAIRLREDSSYEFSTTQSDSIPIVNPTTPGTGGNPYPTINPTSPQPPDTSTGQTPPSPNTGTTSPTNPYSNPPTSTSPYSNPPASTNPYSNPPSSTNPYSNPTSPTTTTPTSPNVTPTTPGTGSSGGGGGGGGGQWCVASESAAETTLKVALDYACGYGADCSQLQQGGACYDPNTLKDHASYAFNDYYQKNPAPTSCVFGGVASLTSKDPSHGNCHFSSSKTTSMSPPTFVSPPTTMTPPSPTTMTPPSSMTPPSMTMPDADGGSSSVYGSPPGGSPNIATSTSYSILLLLTTSLYATLHVQNYV*