AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0325720

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0325720 MtrunA17_Chr2g0304061 94.225 329 17 2 1 328 1 328 6.65e-149 422
Msa0325720 MtrunA17_Chr3g0096451 49.660 147 60 4 95 240 62 195 1.66e-34 127
Msa0325720 MtrunA17_Chr5g0443331 50.000 144 47 4 109 247 75 198 3.72e-33 123
Msa0325720 MtrunA17_Chr1g0193131 45.545 202 79 5 56 236 307 498 1.93e-31 124
Msa0325720 MtrunA17_Chr7g0271411 46.524 187 82 6 56 240 73 243 2.29e-30 117
Msa0325720 MtrunA17_Chr8g0378361 48.031 127 61 2 151 276 23 145 2.35e-29 111
Msa0325720 MtrunA17_Chr8g0381081 55.556 90 38 2 155 242 54 143 1.78e-26 103
Msa0325720 MtrunA17_Chr8g0380231 60.000 80 28 2 167 245 26 102 7.84e-26 99.4
Msa0325720 MtrunA17_Chr5g0447651 55.814 86 36 2 158 241 30 115 7.50e-25 97.1
Msa0325720 MtrunA17_Chr4g0075031 54.348 92 40 2 158 247 382 473 1.11e-24 104
Msa0325720 MtrunA17_Chr8g0378371 54.945 91 39 1 153 243 28 116 3.01e-24 95.5
Msa0325720 MtrunA17_Chr8g0378621 51.000 100 45 3 145 240 349 448 6.74e-23 99.0
Msa0325720 MtrunA17_Chr4g0040911 42.424 132 65 4 148 275 25 149 1.07e-22 93.2
Msa0325720 MtrunA17_Chr8g0378381 48.810 84 42 1 158 240 32 115 1.64e-22 90.9
Msa0325720 MtrunA17_Chr1g0177171 52.273 88 40 2 155 240 403 490 8.48e-22 95.9
Msa0325720 MtrunA17_Chr1g0213651 48.810 84 42 1 158 240 276 359 1.33e-21 94.0
Msa0325720 MtrunA17_Chr3g0120401 48.276 87 44 1 158 243 157 243 2.29e-21 91.3
Msa0325720 MtrunA17_Chr4g0076131 47.253 91 46 2 153 241 23 113 3.41e-21 87.4
Msa0325720 MtrunA17_Chr8g0378401 51.282 78 37 1 158 234 32 109 1.44e-20 85.5
Msa0325720 MtrunA17_Chr1g0154991 45.882 85 45 1 158 241 20 104 2.31e-20 84.7
Msa0325720 MtrunA17_Chr3g0121271 44.231 104 56 2 146 247 350 453 2.51e-20 91.7
Msa0325720 MtrunA17_Chr5g0419121 48.649 74 37 1 155 227 19 92 3.73e-19 85.5
Msa0325720 MtrunA17_Chr1g0161011 50.000 88 42 2 158 243 361 448 4.65e-19 87.8
Msa0325720 MtrunA17_Chr5g0401301 52.326 86 38 3 158 240 329 414 5.37e-19 87.0
Msa0325720 MtrunA17_Chr4g0076141 50.000 80 38 2 157 234 12 91 7.69e-19 80.5
Msa0325720 MtrunA17_Chr8g0389741 48.235 85 42 2 158 240 368 452 8.08e-19 87.0
Msa0325720 MtrunA17_Chr6g0487261 43.820 89 47 2 155 240 366 454 1.12e-18 86.7
Msa0325720 MtrunA17_Chr5g0439251 45.455 99 44 3 155 243 379 477 1.23e-18 86.3
Msa0325720 MtrunA17_Chr4g0043511 42.391 92 52 1 157 247 419 510 4.39e-18 85.1
Msa0325720 MtrunA17_Chr4g0034901 46.154 91 47 2 157 245 374 464 5.45e-18 84.3
Msa0325720 MtrunA17_Chr5g0434591 47.059 85 43 2 158 240 360 444 8.59e-18 84.0
Msa0325720 MtrunA17_Chr3g0110371 46.591 88 45 2 155 240 358 445 3.54e-17 82.0
Msa0325720 MtrunA17_Chr4g0061401 45.238 84 44 2 158 240 24 106 8.07e-17 78.2
Msa0325720 MtrunA17_Chr4g0043151 49.296 71 35 1 158 227 21 91 2.49e-16 76.3
Msa0325720 MtrunA17_Chr3g0144531 35.965 114 60 3 135 245 356 459 4.77e-15 75.5
Msa0325720 MtrunA17_Chr7g0234821 43.182 88 48 2 155 240 371 458 1.24e-14 74.3
Msa0325720 MtrunA17_Chr8g0339191 39.773 88 51 2 155 240 371 458 2.16e-14 73.6
Msa0325720 MtrunA17_Chr7g0223961 37.209 86 53 1 156 240 363 448 4.30e-13 69.7
Msa0325720 MtrunA17_Chr4g0046311 43.902 82 44 2 155 234 370 451 7.87e-13 68.9
Msa0325720 MtrunA17_Chr2g0313891 41.414 99 54 3 146 241 16 113 9.74e-13 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0325720 AT2G30933.1 58.947 95 38 1 154 247 78 172 5.32e-34 125
Msa0325720 AT1G09460.1 59.140 93 37 1 155 247 134 225 3.33e-30 117
Msa0325720 AT2G30933.2 60.976 82 31 1 154 234 78 159 7.69e-30 112
Msa0325720 AT2G30933.3 60.976 82 31 1 154 234 78 159 7.69e-30 112
Msa0325720 AT5G35740.1 52.809 89 37 4 157 241 29 116 2.23e-24 96.3
Msa0325720 AT4G13600.1 43.411 129 60 3 155 270 58 186 2.91e-24 99.4
Msa0325720 AT4G05430.1 47.312 93 47 2 155 245 20 112 3.15e-23 94.0
Msa0325720 AT4G05430.3 47.312 93 47 2 155 245 45 137 3.49e-23 94.7
Msa0325720 AT4G26830.1 41.406 128 64 4 115 240 333 451 2.85e-22 97.4
Msa0325720 AT4G26830.2 41.406 128 64 4 115 240 337 455 3.18e-22 97.1
Msa0325720 AT1G11820.2 49.000 100 49 2 158 255 381 480 3.74e-22 97.4
Msa0325720 AT5G63250.1 46.154 91 45 2 156 242 40 130 1.39e-21 89.4
Msa0325720 AT2G05790.1 54.118 85 37 2 158 240 384 468 1.60e-21 95.1
Msa0325720 AT2G42930.1 47.727 88 45 1 158 245 40 126 2.13e-21 88.6
Msa0325720 AT4G34480.3 47.619 84 43 1 158 240 253 336 2.82e-21 94.0
Msa0325720 AT4G34480.1 47.619 84 43 1 158 240 364 447 3.13e-21 94.7
Msa0325720 AT1G18650.1 45.161 93 50 1 152 243 15 107 3.74e-21 89.7
Msa0325720 AT5G55180.1 50.000 88 42 2 155 240 369 456 9.14e-21 92.8
Msa0325720 AT5G67460.1 49.425 87 42 2 156 240 291 377 1.20e-20 92.0
Msa0325720 AT1G79480.1 46.429 84 44 1 158 240 311 394 1.48e-20 92.0
Msa0325720 AT1G79480.2 46.429 84 44 1 158 240 310 393 1.54e-20 91.7
Msa0325720 AT1G18650.2 45.055 91 49 1 151 240 14 104 1.71e-20 86.3
Msa0325720 AT1G66250.1 49.438 89 43 2 158 244 368 456 3.75e-20 91.3
Msa0325720 AT2G01630.1 50.588 85 40 2 158 240 360 444 3.81e-20 91.3
Msa0325720 AT3G58100.1 47.191 89 45 2 158 244 41 129 6.37e-20 86.3
Msa0325720 AT2G04910.2 47.727 88 44 2 156 241 13 100 9.05e-20 83.6
Msa0325720 AT4G05430.2 48.780 82 40 2 155 234 45 126 1.21e-19 84.0
Msa0325720 AT5G24318.3 37.069 116 65 2 129 243 211 319 1.65e-19 87.8
Msa0325720 AT5G24318.1 37.069 116 65 2 129 243 350 458 2.57e-19 88.6
Msa0325720 AT3G58100.2 45.918 98 45 3 155 244 108 205 3.07e-19 85.9
Msa0325720 AT4G29360.2 47.727 88 44 2 155 240 398 485 9.38e-19 87.4
Msa0325720 AT1G66855.1 43.023 86 47 2 158 241 26 111 9.77e-19 80.9
Msa0325720 AT4G29360.1 47.727 88 44 2 155 240 388 475 1.00e-18 87.4
Msa0325720 AT1G66870.1 44.578 83 44 2 158 238 26 108 1.37e-18 80.5
Msa0325720 AT5G63225.1 44.706 85 46 1 157 240 24 108 1.37e-18 80.5
Msa0325720 AT1G66870.2 44.578 83 44 2 158 238 53 135 2.23e-18 80.9
Msa0325720 AT3G13560.4 42.105 114 62 3 154 265 358 469 2.28e-18 86.3
Msa0325720 AT3G13560.1 42.105 114 62 3 154 265 358 469 2.28e-18 86.3
Msa0325720 AT3G13560.2 42.105 114 62 3 154 265 358 469 2.28e-18 86.3
Msa0325720 AT3G13560.5 42.105 114 62 3 154 265 358 469 2.28e-18 86.3
Msa0325720 AT3G13560.3 42.105 114 62 3 154 265 358 469 2.28e-18 86.3
Msa0325720 AT5G56590.1 47.059 85 43 2 158 240 369 453 2.51e-18 85.9
Msa0325720 AT5G67460.2 50.617 81 38 2 156 234 291 371 2.90e-18 85.1
Msa0325720 AT1G66852.1 42.683 82 46 1 158 238 26 107 3.02e-18 79.3
Msa0325720 AT2G16230.1 42.857 84 47 1 158 240 366 449 3.55e-18 85.5
Msa0325720 AT3G55430.1 36.111 108 59 2 143 240 342 449 4.03e-18 85.1
Msa0325720 AT5G63240.1 42.857 91 48 2 156 242 38 128 5.16e-18 79.3
Msa0325720 AT5G61130.1 40.800 125 72 2 152 275 15 138 6.95e-18 81.3
Msa0325720 AT1G11820.3 51.899 79 36 2 158 234 381 459 8.44e-18 84.3
Msa0325720 AT2G01630.3 50.633 79 37 2 158 234 360 438 1.04e-17 84.0
Msa0325720 AT5G55180.2 50.602 83 39 2 155 235 369 451 1.07e-17 84.0
Msa0325720 AT1G69295.1 50.704 71 33 2 158 227 21 90 3.10e-16 77.0
Msa0325720 AT1G26450.1 49.296 71 34 2 159 228 22 91 3.83e-16 76.3
Msa0325720 AT1G69295.2 50.704 71 33 2 158 227 17 86 5.66e-16 76.3
Msa0325720 AT4G09462.1 37.209 86 53 1 158 242 29 114 5.68e-16 73.6
Msa0325720 AT5G24318.2 37.383 107 59 2 129 234 350 449 7.09e-16 78.6
Msa0325720 AT2G04910.1 46.914 81 41 2 156 234 13 93 1.41e-15 72.8
Msa0325720 AT4G09465.1 36.047 86 54 1 158 242 31 116 2.17e-15 72.0
Msa0325720 AT4G09464.1 36.047 86 54 1 158 242 31 116 2.40e-15 71.6
Msa0325720 AT4G09467.1 36.047 86 54 1 158 242 31 116 2.40e-15 71.6
Msa0325720 AT5G08000.2 42.529 87 49 1 155 240 37 123 5.87e-15 73.2
Msa0325720 AT5G63230.1 39.759 83 49 1 157 238 25 107 1.06e-14 69.7
Msa0325720 AT5G08000.1 42.857 84 47 1 158 240 21 104 1.15e-14 72.0
Msa0325720 AT3G07320.1 44.318 88 47 2 155 240 368 455 1.21e-14 74.7
Msa0325720 AT3G23770.1 43.182 88 48 2 155 240 384 471 2.98e-14 73.6
Msa0325720 AT4G16165.1 38.372 86 51 2 158 241 26 111 3.47e-14 68.6
Msa0325720 AT4G09090.1 36.782 87 54 1 157 242 30 116 5.25e-14 68.2
Msa0325720 AT4G09466.1 33.721 86 56 1 158 242 31 116 2.30e-13 66.2
Msa0325720 AT2G03505.4 34.021 97 47 2 158 254 21 100 2.99e-13 67.4
Msa0325720 AT2G03505.1 34.021 97 47 2 158 254 21 100 2.99e-13 67.4
Msa0325720 AT2G03505.2 34.021 97 47 2 158 254 21 100 2.99e-13 67.4
Msa0325720 AT2G29735.1 43.529 85 45 2 157 238 30 114 4.24e-13 65.5
Msa0325720 AT2G03505.3 34.021 97 47 2 158 254 21 100 5.57e-13 65.1
Msa0325720 AT5G53610.1 41.667 84 47 2 158 240 28 110 1.00e-12 64.3
Msa0325720 AT5G53600.1 39.286 84 49 2 158 240 29 111 1.02e-12 64.3
Msa0325720 AT2G19440.2 42.045 88 43 5 157 240 361 444 1.40e-12 68.6
Msa0325720 AT2G19440.1 42.045 88 43 5 157 240 361 444 1.40e-12 68.6
Msa0325720 AT2G39640.1 34.524 84 54 1 156 238 458 541 2.63e-12 67.8
Msa0325720 AT2G39640.1 33.010 103 61 2 144 238 338 440 1.28e-11 65.9
Msa0325720 AT1G13830.2 39.189 74 44 1 155 227 32 105 5.21e-12 64.7
Msa0325720 AT1G13830.1 39.437 71 42 1 158 227 21 91 1.28e-11 63.5
Msa0325720 AT1G78520.1 38.824 85 50 2 158 241 32 115 2.61e-11 60.5

Find 128 sgRNAs with CRISPR-Local

Find 181 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATGATCTTTCAAAGTATT+TGG 0.103855 2_4:+33586937 None:intergenic
CCTCCTGTTCCTGGTGTTGT+AGG 0.210126 2_4:+33587381 None:intergenic
TTTAGCATTAGCAGATTTAT+AGG 0.211938 2_4:-33586000 Msa0325720:three_prime_UTR
AAATTCTCACCTTGTTGTTT+TGG 0.240571 2_4:+33586542 None:intergenic
AGAAGTTGATGTAGCTATAT+TGG 0.257371 2_4:+33586281 None:intergenic
TTGGATTATGCTTGTGGATA+TGG 0.261053 2_4:-33587003 Msa0325720:CDS
GCACCTACAAGTTGTGTATT+TGG 0.264956 2_4:-33586877 Msa0325720:CDS
AGATTATCTATTTCAGATAA+AGG 0.268892 2_4:-33585928 Msa0325720:three_prime_UTR
ATAAATCTGCTAATGCTAAA+TGG 0.271823 2_4:+33586003 None:intergenic
GGGCTTGTTGTGCCTGTGTT+TGG 0.273624 2_4:+33587285 None:intergenic
GGCTTGTTGTGCCTGTGTTT+GGG 0.275863 2_4:+33587286 None:intergenic
AGCTGCAGAGACTACTTTAA+AGG 0.280134 2_4:-33587031 Msa0325720:CDS
TGAATCTTCTCTTAATCTTA+TGG 0.280733 2_4:+33587452 None:intergenic
GGGCTTGTTGGGTTTGAGTA+TGG 0.288454 2_4:+33587165 None:intergenic
CTATGTCTTTCCCTCTGTTC+AGG 0.289191 2_4:-33588458 Msa0325720:intron
GGTGGTGGTGGCGGCGGTGG+TGG 0.296560 2_4:-33587078 Msa0325720:CDS
GAAGTTGATGTAGCTATATT+GGG 0.302042 2_4:+33586282 None:intergenic
GACTACTTTAAAGGTAGCTT+TGG 0.305217 2_4:-33587022 Msa0325720:CDS
CTTGTTGGAGTTGTTGTAGT+TGG 0.311943 2_4:+33587144 None:intergenic
GCATAGCTATTGATTGAATA+TGG 0.315916 2_4:-33585858 Msa0325720:three_prime_UTR
GGTGGTGGTGGTGGCGGCGG+TGG 0.323269 2_4:-33587081 Msa0325720:CDS
GGTGTTGTGGGAGTCACATT+TGG 0.328603 2_4:+33587120 None:intergenic
GAACTTGGTGGGTTTGAGTA+TGG 0.330703 2_4:+33587195 None:intergenic
AGAAATTAAACCTCAAGAAA+TGG 0.330880 2_4:-33588513 Msa0325720:exon
GAGGTTGGTGGGTTTGAGTA+TGG 0.333201 2_4:+33587255 None:intergenic
GATAACTAAATATTATTTCA+TGG 0.335055 2_4:+33585826 None:intergenic
CATGATCTTTCAAAGTATTT+GGG 0.337641 2_4:+33586938 None:intergenic
TAAGGATTTCCTCCTGTTCC+TGG 0.340078 2_4:+33587372 None:intergenic
GGGTTTGAGTATGGATTTGT+TGG 0.343138 2_4:+33587264 None:intergenic
GTTGTTGTAGTTGGGCTTGT+TGG 0.343594 2_4:+33587153 None:intergenic
GATTATCTATTTCAGATAAA+GGG 0.350069 2_4:-33585927 Msa0325720:three_prime_UTR
TGGAATTCACACAGTCTTCC+AGG 0.351476 2_4:-33586132 Msa0325720:three_prime_UTR
GTTGTGCCTGTGTTTGGGCT+TGG 0.352978 2_4:+33587291 None:intergenic
TGCCTGTGTTTGGGCTTGGT+GGG 0.358963 2_4:+33587295 None:intergenic
CCTCCAAATACACAACTTGT+AGG 0.370834 2_4:+33586874 None:intergenic
AGAAGCTGGTGGGTTTGAAT+AGG 0.373420 2_4:+33587224 None:intergenic
ATATTACACTGACCCTTGAT+TGG 0.376591 2_4:-33586152 Msa0325720:three_prime_UTR
TTGTTGTAGTTGGGCTTGTT+GGG 0.380851 2_4:+33587154 None:intergenic
GTGCCTGTGTTTGGGCTTGG+TGG 0.381610 2_4:+33587294 None:intergenic
GGAGATGTTGGGTTTATAGT+AGG 0.382143 2_4:+33587348 None:intergenic
GGAGTCACATTTGGGCTTGT+TGG 0.384264 2_4:+33587129 None:intergenic
GTTTATGAGCAATGTTTAAG+AGG 0.385649 2_4:+33588563 None:intergenic
GTTGGGTTTATAGTAGGATA+AGG 0.392906 2_4:+33587354 None:intergenic
CCACCAGATGATCCTGTTCC+TGG 0.395410 2_4:+33587099 None:intergenic
TCAGGTGGTTGAGGAGATGT+TGG 0.398484 2_4:+33587336 None:intergenic
CTTAGTTGCAAGAATTCATA+TGG 0.399617 2_4:+33586063 None:intergenic
GCTATATTGGGGCTTCCTCC+TGG 0.414020 2_4:+33586294 None:intergenic
GTGTTGTGGGAGTCACATTT+GGG 0.421820 2_4:+33587121 None:intergenic
GGAGGATCATCATCAGTCTA+TGG 0.429914 2_4:-33586324 Msa0325720:CDS
GAAATTAAACCTCAAGAAAT+GGG 0.433845 2_4:-33588512 Msa0325720:exon
TTGTTGGAGTTGTTGTAGTT+GGG 0.434457 2_4:+33587145 None:intergenic
GTGGTGTCATACTGCTTGGT+GGG 0.437280 2_4:+33586373 None:intergenic
TGAAATCTTGCAAAATCTCC+TGG 0.440152 2_4:+33586114 None:intergenic
GAAGCTGGTGGGTTTGAATA+GGG 0.441448 2_4:+33587225 None:intergenic
CAGGTGGTTGAGGAGATGTT+GGG 0.444649 2_4:+33587337 None:intergenic
GGATCATCTGGTGGTGGTGG+TGG 0.445151 2_4:-33587090 Msa0325720:CDS
GGTTTGAGTATGGATTTGTT+GGG 0.453799 2_4:+33587265 None:intergenic
TCTATATTGTTTCAGGTCAT+GGG 0.461326 2_4:-33586584 Msa0325720:intron
GAGTATGGATTTGTAGAAGC+TGG 0.464844 2_4:+33587210 None:intergenic
GAGTATGGATTTGTTGAACT+TGG 0.470965 2_4:+33587180 None:intergenic
CTTGGTGGGGTCATAGTAGT+AGG 0.472356 2_4:+33586387 None:intergenic
CTTCTAGCTCCCATTTCTTG+AGG 0.477633 2_4:+33588503 None:intergenic
AATCCTACAACACCAGGAAC+AGG 0.478945 2_4:-33587384 Msa0325720:CDS
GCCTGTGTTTGGGCTTGGTG+GGG 0.479346 2_4:+33587296 None:intergenic
GGTGGTGTCATACTGCTTGG+TGG 0.479946 2_4:+33586372 None:intergenic
GATCCTGTTCCTGGTGTTGT+GGG 0.480455 2_4:+33587108 None:intergenic
ACACCAGGAACAGGATCATC+TGG 0.480901 2_4:-33587102 Msa0325720:CDS
GTTTGTCCAGTACTTGTGTC+AGG 0.483873 2_4:+33587318 None:intergenic
AGGGACTTGTAGAGGTTGGT+GGG 0.484204 2_4:+33587244 None:intergenic
TATGGATTTGTTGAACTTGG+TGG 0.489297 2_4:+33587183 None:intergenic
GTGGCGGCGGTGGTGGTCAG+TGG 0.491010 2_4:-33587071 Msa0325720:CDS
ACTCCCACAACACCAGGAAC+AGG 0.492125 2_4:-33587111 Msa0325720:CDS
GATGATCCTCCATCAGCATC+AGG 0.496560 2_4:+33586336 None:intergenic
GAATAGGGACTTGTAGAGGT+TGG 0.497717 2_4:+33587240 None:intergenic
TAGGGACTTGTAGAGGTTGG+TGG 0.498204 2_4:+33587243 None:intergenic
GTACTTGTGTCAGGTGGTTG+AGG 0.502277 2_4:+33587327 None:intergenic
ACAGGATCATCTGGTGGTGG+TGG 0.506368 2_4:-33587093 Msa0325720:CDS
GTAGCTTTGGATTATGCTTG+TGG 0.506964 2_4:-33587009 Msa0325720:CDS
GGTGTTGTAGGATTCACAAT+AGG 0.507724 2_4:+33587393 None:intergenic
GACTGTGTGAATTCCAATCA+AGG 0.510660 2_4:+33586139 None:intergenic
TGATCCTGTTCCTGGTGTTG+TGG 0.511505 2_4:+33587107 None:intergenic
AAGATTAAATTCTCCATCAC+AGG 0.523019 2_4:-33588626 Msa0325720:five_prime_UTR
CTAGGTGGAGTCATAGTAGT+TGG 0.526635 2_4:+33586411 None:intergenic
ATGGATTTGTTGAACTTGGT+GGG 0.527334 2_4:+33587184 None:intergenic
CTTGGTGGTGTCATACTGCT+TGG 0.530201 2_4:+33586369 None:intergenic
TCAGTCTATGGTTCACCACC+AGG 0.530618 2_4:-33586312 Msa0325720:CDS
ATGGATTTGTAGAAGCTGGT+GGG 0.535192 2_4:+33587214 None:intergenic
GCATCAGGCATTGTCATGCT+TGG 0.540422 2_4:+33586351 None:intergenic
CAACCACCTGACACAAGTAC+TGG 0.540527 2_4:-33587324 Msa0325720:CDS
GCACATGAAGTGTAGCATAG+AGG 0.541615 2_4:+33586229 None:intergenic
AAGAAATGGGAGCTAGAAGT+TGG 0.544857 2_4:-33588499 Msa0325720:exon
TCAGGCATTGTCATGCTTGG+TGG 0.555903 2_4:+33586354 None:intergenic
GCTATTGATTGAATATGGAA+TGG 0.558429 2_4:-33585853 Msa0325720:three_prime_UTR
CATTGACATGTGTCAGACAC+TGG 0.559610 2_4:-33586182 Msa0325720:three_prime_UTR
ACATTGCTCATAAACTTGAA+AGG 0.561393 2_4:-33588555 Msa0325720:five_prime_UTR
TGTAGTGAGTAGTAGCAGAA+TGG 0.563778 2_4:+33586254 None:intergenic
GTGGAGTCATAGTAGTTGGT+GGG 0.565923 2_4:+33586415 None:intergenic
CCTACAAGTTGTGTATTTGG+AGG 0.565962 2_4:-33586874 Msa0325720:CDS
AATGTGACTCCCACAACACC+AGG 0.569381 2_4:-33587117 Msa0325720:CDS
TAAAATCACACCTGAACAGA+GGG 0.572156 2_4:+33588448 None:intergenic
AATACACAACTTGTAGGTGC+AGG 0.573202 2_4:+33586880 None:intergenic
ACCCCACCAAGCCCAAACAC+AGG 0.573627 2_4:-33587297 Msa0325720:CDS
GGTGGAGTCATAGTAGTTGG+TGG 0.575064 2_4:+33586414 None:intergenic
ATAAAATCACACCTGAACAG+AGG 0.579833 2_4:+33588447 None:intergenic
TTGCAATGAAGTTCCTGTGA+TGG 0.600039 2_4:+33588613 None:intergenic
GTTGGTGGGCTTACAAATGT+TGG 0.600377 2_4:+33586429 None:intergenic
TGTCCAGTACTTGTGTCAGG+TGG 0.608903 2_4:+33587321 None:intergenic
GGGGTCATAGTAGTAGGACT+AGG 0.609330 2_4:+33586393 None:intergenic
ATGACAATGCCTGATGCTGA+TGG 0.613075 2_4:-33586345 Msa0325720:CDS
AAGTTGATGTAGCTATATTG+GGG 0.613710 2_4:+33586283 None:intergenic
GGTGGGCTTACAAATGTTGG+TGG 0.618577 2_4:+33586432 None:intergenic
GGAACAGGATCATCTGGTGG+TGG 0.620332 2_4:-33587096 Msa0325720:CDS
TAATACTATTGATGAAAACA+TGG 0.622567 2_4:-33585895 Msa0325720:three_prime_UTR
TATGGATTTGTAGAAGCTGG+TGG 0.627475 2_4:+33587213 None:intergenic
TAAATGGATACATTGATGCA+AGG 0.637168 2_4:+33586019 None:intergenic
TGGTGTCATACTGCTTGGTG+GGG 0.639890 2_4:+33586374 None:intergenic
ATATTGGGGCTTCCTCCTGG+TGG 0.641948 2_4:+33586297 None:intergenic
ATTGTGAATCCTACAACACC+AGG 0.643642 2_4:-33587390 Msa0325720:CDS
CCTACAACACCAGGAACAGG+AGG 0.644858 2_4:-33587381 Msa0325720:CDS
GTTTGAATAGGGACTTGTAG+AGG 0.646608 2_4:+33587236 None:intergenic
GTCATAGTAGTAGGACTAGG+TGG 0.650747 2_4:+33586396 None:intergenic
ACTGTGTGAATTCCAATCAA+GGG 0.659940 2_4:+33586140 None:intergenic
ACAATGCCTGATGCTGATGG+AGG 0.696819 2_4:-33586342 Msa0325720:CDS
TCTCATCCTCCAAAACAACA+AGG 0.698612 2_4:-33586551 Msa0325720:intron
CCAGGAACAGGATCATCTGG+TGG 0.699045 2_4:-33587099 Msa0325720:CDS
GATTGTTCACAACTTCAACA+AGG 0.714222 2_4:-33586976 Msa0325720:CDS
GTCTATGGTTCACCACCAGG+AGG 0.724042 2_4:-33586309 Msa0325720:CDS
TGTTCACAACTTCAACAAGG+AGG 0.728282 2_4:-33586973 Msa0325720:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTTATAGATTCAATTT+TGG + chr2_4:33586511-33586530 None:intergenic 10.0%
!!! AAAATTAAGTTGTATGTATT+TGG - chr2_4:33586289-33586308 Msa0325720:CDS 15.0%
!! ACTTATTTATTAATTCAATC+AGG - chr2_4:33586919-33586938 Msa0325720:CDS 15.0%
!!! ATATCTATATTTTGTTTTAC+AGG - chr2_4:33586984-33587003 Msa0325720:CDS 15.0%
!! GTAGAGAAATATATATTGAA+AGG + chr2_4:33586071-33586090 None:intergenic 20.0%
!!! TATATTTCTCTACGTTTTAT+AGG - chr2_4:33586078-33586097 Msa0325720:three_prime_UTR 20.0%
!!! ACTGATTTTAAATTGAAGTT+GGG + chr2_4:33586773-33586792 None:intergenic 20.0%
!!! TACTGATTTTAAATTGAAGT+TGG + chr2_4:33586774-33586793 None:intergenic 20.0%
!! AGGCATATATAAACATATAT+AGG - chr2_4:33586869-33586888 Msa0325720:CDS 20.0%
!!! ATATTTTGTTTTACAGGTTT+AGG - chr2_4:33586990-33587009 Msa0325720:CDS 20.0%
!! ATCATGATGAATTGATATTA+AGG + chr2_4:33587677-33587696 None:intergenic 20.0%
!!! AGTTTTATGCAATACTAATA+AGG + chr2_4:33587975-33587994 None:intergenic 20.0%
!! AGATTATCTATTTCAGATAA+AGG - chr2_4:33588542-33588561 Msa0325720:five_prime_UTR 20.0%
!! GATTATCTATTTCAGATAAA+GGG - chr2_4:33588543-33588562 Msa0325720:five_prime_UTR 20.0%
!! TAATACTATTGATGAAAACA+TGG - chr2_4:33588575-33588594 Msa0325720:five_prime_UTR 20.0%
! AGAAATTAAACCTCAAGAAA+TGG - chr2_4:33585957-33585976 Msa0325720:three_prime_UTR 25.0%
! GAAATTAAACCTCAAGAAAT+GGG - chr2_4:33585958-33585977 Msa0325720:three_prime_UTR 25.0%
! ATATCCAAAATGACAACATT+TGG - chr2_4:33586572-33586591 Msa0325720:CDS 25.0%
!! TCAACTGAATAAGTTGAATT+AGG - chr2_4:33586815-33586834 Msa0325720:intron 25.0%
! AGGATGAACTTATTAACATA+AGG - chr2_4:33586835-33586854 Msa0325720:intron 25.0%
! TATTAACATAAGGTAGTTGT+AGG - chr2_4:33586845-33586864 Msa0325720:CDS 25.0%
! TGAATCTTCTCTTAATCTTA+TGG + chr2_4:33587021-33587040 None:intergenic 25.0%
!! CATGATCTTTCAAAGTATTT+GGG + chr2_4:33587535-33587554 None:intergenic 25.0%
! TGTTGTATGAATATCTTACT+TGG + chr2_4:33587647-33587666 None:intergenic 25.0%
! AGATATTCATACAACATTGA+AGG - chr2_4:33587651-33587670 Msa0325720:intron 25.0%
! AATTCATCATGATCATTGAA+CGG - chr2_4:33587684-33587703 Msa0325720:intron 25.0%
! AACTTGATTTAGATCATGTT+TGG + chr2_4:33587851-33587870 None:intergenic 25.0%
!!! GTTGTTTTCTATATTGTTTC+AGG - chr2_4:33587879-33587898 Msa0325720:intron 25.0%
! TAAGGTGAATATGATATAAG+TGG + chr2_4:33587957-33587976 None:intergenic 25.0%
! AAGGACAATACATATATTAG+AGG + chr2_4:33588002-33588021 None:intergenic 25.0%
! ATAAATCTGCTAATGCTAAA+TGG + chr2_4:33588470-33588489 None:intergenic 25.0%
! TTTAGCATTAGCAGATTTAT+AGG - chr2_4:33588470-33588489 Msa0325720:CDS 25.0%
AAGATTAAATTCTCCATCAC+AGG - chr2_4:33585844-33585863 Msa0325720:three_prime_UTR 30.0%
GTTTATGAGCAATGTTTAAG+AGG + chr2_4:33585910-33585929 None:intergenic 30.0%
ACATTGCTCATAAACTTGAA+AGG - chr2_4:33585915-33585934 Msa0325720:three_prime_UTR 30.0%
GACATAGCAAAAATAAGACA+AGG + chr2_4:33585999-33586018 None:intergenic 30.0%
AATAGTTGTGTTGTGTGATA+AGG - chr2_4:33586129-33586148 Msa0325720:three_prime_UTR 30.0%
TAGAAAAACAAGCATGTACA+TGG + chr2_4:33586543-33586562 None:intergenic 30.0%
!! GAATCCAAATGTTGTCATTT+TGG + chr2_4:33586579-33586598 None:intergenic 30.0%
TGACAACATTTGGATTCATT+AGG - chr2_4:33586582-33586601 Msa0325720:intron 30.0%
! GCATGATCTTTCAAAGTATT+TGG + chr2_4:33587536-33587555 None:intergenic 30.0%
! TTCTATATTGTTTCAGGTCA+TGG - chr2_4:33587885-33587904 Msa0325720:intron 30.0%
! TCTATATTGTTTCAGGTCAT+GGG - chr2_4:33587886-33587905 Msa0325720:intron 30.0%
!!! AAATTCTCACCTTGTTGTTT+TGG + chr2_4:33587931-33587950 None:intergenic 30.0%
!! AAGTTGATGTAGCTATATTG+GGG + chr2_4:33588190-33588209 None:intergenic 30.0%
!! GAAGTTGATGTAGCTATATT+GGG + chr2_4:33588191-33588210 None:intergenic 30.0%
!! AGAAGTTGATGTAGCTATAT+TGG + chr2_4:33588192-33588211 None:intergenic 30.0%
CTTAGTTGCAAGAATTCATA+TGG + chr2_4:33588410-33588429 None:intergenic 30.0%
TAAATGGATACATTGATGCA+AGG + chr2_4:33588454-33588473 None:intergenic 30.0%
! GCATAGCTATTGATTGAATA+TGG - chr2_4:33588612-33588631 Msa0325720:five_prime_UTR 30.0%
! GCTATTGATTGAATATGGAA+TGG - chr2_4:33588617-33588636 Msa0325720:five_prime_UTR 30.0%
TAAAATCACACCTGAACAGA+GGG + chr2_4:33586025-33586044 None:intergenic 35.0%
ATAAAATCACACCTGAACAG+AGG + chr2_4:33586026-33586045 None:intergenic 35.0%
TGTTGTGTGATAAGGAGTTA+AGG - chr2_4:33586137-33586156 Msa0325720:three_prime_UTR 35.0%
TTGAAACATGTAAAGCTGCA+GGG - chr2_4:33586227-33586246 Msa0325720:CDS 35.0%
TAATATTACGACCGAGATCA+TGG + chr2_4:33586662-33586681 None:intergenic 35.0%
TGATCTCGGTCGTAATATTA+AGG - chr2_4:33586662-33586681 Msa0325720:intron 35.0%
TTGTAACACAACTGTGATTG+AGG - chr2_4:33586703-33586722 Msa0325720:intron 35.0%
AGTTGGGATCTTCGATATTA+TGG + chr2_4:33586757-33586776 None:intergenic 35.0%
AACATAAGGTAGTTGTAGGA+AGG - chr2_4:33586849-33586868 Msa0325720:CDS 35.0%
GTTGGGTTTATAGTAGGATA+AGG + chr2_4:33587119-33587138 None:intergenic 35.0%
! GGTTTGAGTATGGATTTGTT+GGG + chr2_4:33587208-33587227 None:intergenic 35.0%
!! ATGGATTTGTTGAACTTGGT+GGG + chr2_4:33587289-33587308 None:intergenic 35.0%
!! TATGGATTTGTTGAACTTGG+TGG + chr2_4:33587290-33587309 None:intergenic 35.0%
!! GAGTATGGATTTGTTGAACT+TGG + chr2_4:33587293-33587312 None:intergenic 35.0%
TTGTTGGAGTTGTTGTAGTT+GGG + chr2_4:33587328-33587347 None:intergenic 35.0%
! GACTACTTTAAAGGTAGCTT+TGG - chr2_4:33587448-33587467 Msa0325720:CDS 35.0%
TTGGATTATGCTTGTGGATA+TGG - chr2_4:33587467-33587486 Msa0325720:CDS 35.0%
GATTGTTCACAACTTCAACA+AGG - chr2_4:33587494-33587513 Msa0325720:intron 35.0%
ACATGTGTTAATGTTGTGTC+AGG + chr2_4:33587744-33587763 None:intergenic 35.0%
TGACACAACATTAACACATG+TGG - chr2_4:33587743-33587762 Msa0325720:intron 35.0%
ATATTACACTGACCCTTGAT+TGG - chr2_4:33588318-33588337 Msa0325720:intron 35.0%
ACTGTGTGAATTCCAATCAA+GGG + chr2_4:33588333-33588352 None:intergenic 35.0%
TGAAATCTTGCAAAATCTCC+TGG + chr2_4:33588359-33588378 None:intergenic 35.0%
TTGCAATGAAGTTCCTGTGA+TGG + chr2_4:33585860-33585879 None:intergenic 40.0%
AAGAAATGGGAGCTAGAAGT+TGG - chr2_4:33585971-33585990 Msa0325720:three_prime_UTR 40.0%
GTTGAAACATGTAAAGCTGC+AGG - chr2_4:33586226-33586245 Msa0325720:CDS 40.0%
! TTTTTCCATTCCGCAGTCAT+AGG - chr2_4:33586630-33586649 Msa0325720:intron 40.0%
ATAGGACACATCCATGATCT+CGG - chr2_4:33586648-33586667 Msa0325720:intron 40.0%
CGCGGTTAAATTCAGTTGAA+CGG + chr2_4:33586729-33586748 None:intergenic 40.0%
TTCGATATTATGGCAGATCG+CGG + chr2_4:33586747-33586766 None:intergenic 40.0%
! GGTGTTGTAGGATTCACAAT+AGG + chr2_4:33587080-33587099 None:intergenic 40.0%
ATTGTGAATCCTACAACACC+AGG - chr2_4:33587080-33587099 Msa0325720:CDS 40.0%
GGAGATGTTGGGTTTATAGT+AGG + chr2_4:33587125-33587144 None:intergenic 40.0%
GGGTTTGAGTATGGATTTGT+TGG + chr2_4:33587209-33587228 None:intergenic 40.0%
GTTTGAATAGGGACTTGTAG+AGG + chr2_4:33587237-33587256 None:intergenic 40.0%
ATGGATTTGTAGAAGCTGGT+GGG + chr2_4:33587259-33587278 None:intergenic 40.0%
TATGGATTTGTAGAAGCTGG+TGG + chr2_4:33587260-33587279 None:intergenic 40.0%
GAGTATGGATTTGTAGAAGC+TGG + chr2_4:33587263-33587282 None:intergenic 40.0%
! TTGTTGTAGTTGGGCTTGTT+GGG + chr2_4:33587319-33587338 None:intergenic 40.0%
CTTGTTGGAGTTGTTGTAGT+TGG + chr2_4:33587329-33587348 None:intergenic 40.0%
AGCTGCAGAGACTACTTTAA+AGG - chr2_4:33587439-33587458 Msa0325720:CDS 40.0%
!! GTAGCTTTGGATTATGCTTG+TGG - chr2_4:33587461-33587480 Msa0325720:CDS 40.0%
TGTTCACAACTTCAACAAGG+AGG - chr2_4:33587497-33587516 Msa0325720:intron 40.0%
AATACACAACTTGTAGGTGC+AGG + chr2_4:33587593-33587612 None:intergenic 40.0%
GCACCTACAAGTTGTGTATT+TGG - chr2_4:33587593-33587612 Msa0325720:intron 40.0%
CCTCCAAATACACAACTTGT+AGG + chr2_4:33587599-33587618 None:intergenic 40.0%
CCTACAAGTTGTGTATTTGG+AGG - chr2_4:33587596-33587615 Msa0325720:intron 40.0%
!!! TTGTTTTGGAGGATGAGAAG+TGG + chr2_4:33587917-33587936 None:intergenic 40.0%
TCTCATCCTCCAAAACAACA+AGG - chr2_4:33587919-33587938 Msa0325720:intron 40.0%
!!! TTCTCACCTTGTTGTTTTGG+AGG + chr2_4:33587928-33587947 None:intergenic 40.0%
TGTAGTGAGTAGTAGCAGAA+TGG + chr2_4:33588219-33588238 None:intergenic 40.0%
GACTGTGTGAATTCCAATCA+AGG + chr2_4:33588334-33588353 None:intergenic 40.0%
!! CTTCTAGCTCCCATTTCTTG+AGG + chr2_4:33585970-33585989 None:intergenic 45.0%
CTATGTCTTTCCCTCTGTTC+AGG - chr2_4:33586012-33586031 Msa0325720:three_prime_UTR 45.0%
ATGGATGTGTCCTATGACTG+CGG + chr2_4:33586643-33586662 None:intergenic 45.0%
AATCCTACAACACCAGGAAC+AGG - chr2_4:33587086-33587105 Msa0325720:CDS 45.0%
TAAGGATTTCCTCCTGTTCC+TGG + chr2_4:33587101-33587120 None:intergenic 45.0%
GTTTGTCCAGTACTTGTGTC+AGG + chr2_4:33587155-33587174 None:intergenic 45.0%
GAATAGGGACTTGTAGAGGT+TGG + chr2_4:33587233-33587252 None:intergenic 45.0%
!! GAAGCTGGTGGGTTTGAATA+GGG + chr2_4:33587248-33587267 None:intergenic 45.0%
!! AGAAGCTGGTGGGTTTGAAT+AGG + chr2_4:33587249-33587268 None:intergenic 45.0%
!! GAACTTGGTGGGTTTGAGTA+TGG + chr2_4:33587278-33587297 None:intergenic 45.0%
GTTGTTGTAGTTGGGCTTGT+TGG + chr2_4:33587320-33587339 None:intergenic 45.0%
GTGTTGTGGGAGTCACATTT+GGG + chr2_4:33587352-33587371 None:intergenic 45.0%
GATCATTGAACGGTAGTGTC+TGG - chr2_4:33587694-33587713 Msa0325720:intron 45.0%
GTCTGGTATCCGACATGTAT+CGG - chr2_4:33587711-33587730 Msa0325720:intron 45.0%
!! GTTGGTGGGCTTACAAATGT+TGG + chr2_4:33588044-33588063 None:intergenic 45.0%
GTGGAGTCATAGTAGTTGGT+GGG + chr2_4:33588058-33588077 None:intergenic 45.0%
CTAGGTGGAGTCATAGTAGT+TGG + chr2_4:33588062-33588081 None:intergenic 45.0%
GTCATAGTAGTAGGACTAGG+TGG + chr2_4:33588077-33588096 None:intergenic 45.0%
ATGACAATGCCTGATGCTGA+TGG - chr2_4:33588125-33588144 Msa0325720:intron 45.0%
GGAGGATCATCATCAGTCTA+TGG - chr2_4:33588146-33588165 Msa0325720:intron 45.0%
GCACATGAAGTGTAGCATAG+AGG + chr2_4:33588244-33588263 None:intergenic 45.0%
CATTGACATGTGTCAGACAC+TGG - chr2_4:33588288-33588307 Msa0325720:intron 45.0%
TGGAATTCACACAGTCTTCC+AGG - chr2_4:33588338-33588357 Msa0325720:intron 45.0%
!!! ATTTTATTTTTTAAATACTT+TGG - chr2_4:33586044-33586063 Msa0325720:three_prime_UTR 5.0%
TGTGTCCTATGACTGCGGAA+TGG + chr2_4:33586638-33586657 None:intergenic 50.0%
CAGGTGGTTGAGGAGATGTT+GGG + chr2_4:33587136-33587155 None:intergenic 50.0%
TCAGGTGGTTGAGGAGATGT+TGG + chr2_4:33587137-33587156 None:intergenic 50.0%
GTACTTGTGTCAGGTGGTTG+AGG + chr2_4:33587146-33587165 None:intergenic 50.0%
CAACCACCTGACACAAGTAC+TGG - chr2_4:33587146-33587165 Msa0325720:CDS 50.0%
TGTCCAGTACTTGTGTCAGG+TGG + chr2_4:33587152-33587171 None:intergenic 50.0%
!! GGCTTGTTGTGCCTGTGTTT+GGG + chr2_4:33587187-33587206 None:intergenic 50.0%
!! GAGGTTGGTGGGTTTGAGTA+TGG + chr2_4:33587218-33587237 None:intergenic 50.0%
! AGGGACTTGTAGAGGTTGGT+GGG + chr2_4:33587229-33587248 None:intergenic 50.0%
TAGGGACTTGTAGAGGTTGG+TGG + chr2_4:33587230-33587249 None:intergenic 50.0%
!! GGGCTTGTTGGGTTTGAGTA+TGG + chr2_4:33587308-33587327 None:intergenic 50.0%
GGAGTCACATTTGGGCTTGT+TGG + chr2_4:33587344-33587363 None:intergenic 50.0%
! GGTGTTGTGGGAGTCACATT+TGG + chr2_4:33587353-33587372 None:intergenic 50.0%
AATGTGACTCCCACAACACC+AGG - chr2_4:33587353-33587372 Msa0325720:CDS 50.0%
!! GATCCTGTTCCTGGTGTTGT+GGG + chr2_4:33587365-33587384 None:intergenic 50.0%
! TGATCCTGTTCCTGGTGTTG+TGG + chr2_4:33587366-33587385 None:intergenic 50.0%
ACACCAGGAACAGGATCATC+TGG - chr2_4:33587368-33587387 Msa0325720:CDS 50.0%
GGTCAGACACCGATACATGT+CGG + chr2_4:33587723-33587742 None:intergenic 50.0%
TGGACTCATGCTGCAATGCA+AGG + chr2_4:33588021-33588040 None:intergenic 50.0%
! GGTGGGCTTACAAATGTTGG+TGG + chr2_4:33588041-33588060 None:intergenic 50.0%
GGTGGAGTCATAGTAGTTGG+TGG + chr2_4:33588059-33588078 None:intergenic 50.0%
GGGGTCATAGTAGTAGGACT+AGG + chr2_4:33588080-33588099 None:intergenic 50.0%
CTTGGTGGGGTCATAGTAGT+AGG + chr2_4:33588086-33588105 None:intergenic 50.0%
!! TGGTGTCATACTGCTTGGTG+GGG + chr2_4:33588099-33588118 None:intergenic 50.0%
!! GTGGTGTCATACTGCTTGGT+GGG + chr2_4:33588100-33588119 None:intergenic 50.0%
!! CTTGGTGGTGTCATACTGCT+TGG + chr2_4:33588104-33588123 None:intergenic 50.0%
! TCAGGCATTGTCATGCTTGG+TGG + chr2_4:33588119-33588138 None:intergenic 50.0%
! GCATCAGGCATTGTCATGCT+TGG + chr2_4:33588122-33588141 None:intergenic 50.0%
ACAATGCCTGATGCTGATGG+AGG - chr2_4:33588128-33588147 Msa0325720:intron 50.0%
GATGATCCTCCATCAGCATC+AGG + chr2_4:33588137-33588156 None:intergenic 50.0%
TCAGTCTATGGTTCACCACC+AGG - chr2_4:33588158-33588177 Msa0325720:intron 50.0%
!! CCTCCTGTTCCTGGTGTTGT+AGG + chr2_4:33587092-33587111 None:intergenic 55.0%
CCTACAACACCAGGAACAGG+AGG - chr2_4:33587089-33587108 Msa0325720:CDS 55.0%
! TGCCTGTGTTTGGGCTTGGT+GGG + chr2_4:33587178-33587197 None:intergenic 55.0%
GTTGTGCCTGTGTTTGGGCT+TGG + chr2_4:33587182-33587201 None:intergenic 55.0%
!! GGGCTTGTTGTGCCTGTGTT+TGG + chr2_4:33587188-33587207 None:intergenic 55.0%
ACTCCCACAACACCAGGAAC+AGG - chr2_4:33587359-33587378 Msa0325720:CDS 55.0%
CCACCAGATGATCCTGTTCC+TGG + chr2_4:33587374-33587393 None:intergenic 55.0%
CCAGGAACAGGATCATCTGG+TGG - chr2_4:33587371-33587390 Msa0325720:CDS 55.0%
GGAACAGGATCATCTGGTGG+TGG - chr2_4:33587374-33587393 Msa0325720:CDS 55.0%
!! ACAGGATCATCTGGTGGTGG+TGG - chr2_4:33587377-33587396 Msa0325720:CDS 55.0%
!! GGTGGTGTCATACTGCTTGG+TGG + chr2_4:33588101-33588120 None:intergenic 55.0%
GTCTATGGTTCACCACCAGG+AGG - chr2_4:33588161-33588180 Msa0325720:intron 55.0%
ATATTGGGGCTTCCTCCTGG+TGG + chr2_4:33588176-33588195 None:intergenic 55.0%
GCTATATTGGGGCTTCCTCC+TGG + chr2_4:33588179-33588198 None:intergenic 55.0%
ACCCCACCAAGCCCAAACAC+AGG - chr2_4:33587173-33587192 Msa0325720:CDS 60.0%
!! GCCTGTGTTTGGGCTTGGTG+GGG + chr2_4:33587177-33587196 None:intergenic 60.0%
GTGCCTGTGTTTGGGCTTGG+TGG + chr2_4:33587179-33587198 None:intergenic 60.0%
!! GGATCATCTGGTGGTGGTGG+TGG - chr2_4:33587380-33587399 Msa0325720:CDS 60.0%
!! TCATCTGGTGGTGGTGGTGG+CGG - chr2_4:33587383-33587402 Msa0325720:CDS 60.0%
!! TCTGGTGGTGGTGGTGGCGG+CGG - chr2_4:33587386-33587405 Msa0325720:CDS 70.0%
! GTGGCGGCGGTGGTGGTCAG+TGG - chr2_4:33587399-33587418 Msa0325720:CDS 75.0%
!!! GGTGGTGGTGGTGGCGGCGG+TGG - chr2_4:33587389-33587408 Msa0325720:CDS 80.0%
!!! GGTGGTGGTGGCGGCGGTGG+TGG - chr2_4:33587392-33587411 Msa0325720:CDS 80.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 33585840 33588652 33585840 ID=Msa0325720;Name=Msa0325720
chr2_4 mRNA 33585840 33588652 33585840 ID=Msa0325720-mRNA-1;Parent=Msa0325720;Name=Msa0325720-mRNA-1;_AED=0.03;_eAED=0.03;_QI=136|1|0.75|1|1|1|4|375|328
chr2_4 exon 33585840 33586461 33585840 ID=Msa0325720-mRNA-1:exon:10786;Parent=Msa0325720-mRNA-1
chr2_4 exon 33586552 33586591 33586552 ID=Msa0325720-mRNA-1:exon:10785;Parent=Msa0325720-mRNA-1
chr2_4 exon 33586845 33587486 33586845 ID=Msa0325720-mRNA-1:exon:10784;Parent=Msa0325720-mRNA-1
chr2_4 exon 33588459 33588652 33588459 ID=Msa0325720-mRNA-1:exon:10783;Parent=Msa0325720-mRNA-1
chr2_4 five_prime_UTR 33588517 33588652 33588517 ID=Msa0325720-mRNA-1:five_prime_utr;Parent=Msa0325720-mRNA-1
chr2_4 CDS 33588459 33588516 33588459 ID=Msa0325720-mRNA-1:cds;Parent=Msa0325720-mRNA-1
chr2_4 CDS 33586845 33587486 33586845 ID=Msa0325720-mRNA-1:cds;Parent=Msa0325720-mRNA-1
chr2_4 CDS 33586552 33586591 33586552 ID=Msa0325720-mRNA-1:cds;Parent=Msa0325720-mRNA-1
chr2_4 CDS 33586215 33586461 33586215 ID=Msa0325720-mRNA-1:cds;Parent=Msa0325720-mRNA-1
chr2_4 three_prime_UTR 33585840 33586214 33585840 ID=Msa0325720-mRNA-1:three_prime_utr;Parent=Msa0325720-mRNA-1
Gene Sequence

>Msa0325720

ATGGGAGCTAGAAGTTGGCACCTTGTCTTATTTTTGCTATGTCTTTCCCTCTGTTCAGGTTTAGGTACAGCCATAAGATTAAGAGAAGATTCATCATATGAATTTAGTACAACTCAATCAGATTCAATTCCTATTGTGAATCCTACAACACCAGGAACAGGAGGAAATCCTTATCCTACTATAAACCCAACATCTCCTCAACCACCTGACACAAGTACTGGACAAACCCCACCAAGCCCAAACACAGGCACAACAAGCCCAACAAATCCATACTCAAACCCACCAACCTCTACAAGTCCCTATTCAAACCCACCAGCTTCTACAAATCCATACTCAAACCCACCAAGTTCAACAAATCCATACTCAAACCCAACAAGCCCAACTACAACAACTCCAACAAGCCCAAATGTGACTCCCACAACACCAGGAACAGGATCATCTGGTGGTGGTGGTGGCGGCGGTGGTGGTCAGTGGTGTGTTGCAAGTGAATCAGCTGCAGAGACTACTTTAAAGGTAGCTTTGGATTATGCTTGTGGATATGGTGCAGATTGTTCACAACTTCAACAAGGAGGAGCTTGTTATGACCCAAATACTTTGAAAGATCATGCTTCTTATGCATTCAATGATTACTATCAAAAGAATCCTGCACCTACAAGTTGTGTATTTGGAGGAGTAGCATCACTCACAAGCAAAGATCCAAGTCATGGGAATTGCCACTTCTCATCCTCCAAAACAACAAGCATGAGTCCACCAACATTTGTAAGCCCACCAACTACTATGACTCCACCTAGTCCTACTACTATGACCCCACCAAGCAGTATGACACCACCAAGCATGACAATGCCTGATGCTGATGGAGGATCATCATCAGTCTATGGTTCACCACCAGGAGGAAGCCCCAATATAGCTACATCAACTTCTTATTCCATTCTGCTACTACTCACTACAAGCCTCTATGCTACACTTCATGTGCAAAATTATGTCTAG
Protein sequence

>Msa0325720

MGARSWHLVLFLLCLSLCSGLGTAIRLREDSSYEFSTTQSDSIPIVNPTTPGTGGNPYPTINPTSPQPPDTSTGQTPPSPNTGTTSPTNPYSNPPTSTSPYSNPPASTNPYSNPPSSTNPYSNPTSPTTTTPTSPNVTPTTPGTGSSGGGGGGGGGQWCVASESAAETTLKVALDYACGYGADCSQLQQGGACYDPNTLKDHASYAFNDYYQKNPAPTSCVFGGVASLTSKDPSHGNCHFSSSKTTSMSPPTFVSPPTTMTPPSPTTMTPPSSMTPPSMTMPDADGGSSSVYGSPPGGSPNIATSTSYSILLLLTTSLYATLHVQNYV*