AlfalfaGEDB | Msa0263630

M. sativa cultivar zhongmu-4 / Msa0263630

KEGG: K05391
GO: GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005249 GO:0005261 GO:0005262 GO:0005267 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006816 GO:0008150 GO:0008324 GO:0009877 GO:0009987 GO:0012505 GO:0015075 GO:0015077 GO:0015079 GO:0015085 GO:0015267 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0031965 GO:0031967 GO:0031975 GO:0034220 GO:0036377 GO:0042391 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044325 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0051704 GO:0055085 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071804 GO:0071805 GO:0071944 GO:0072511 GO:0098655 GO:0098660 GO:0098662
PFAM: PF00520.34

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0263630	XP_013465310.1	56.604	159	56	4	1	158	1	147	5.57e-34	149
Msa0263630	XP_013465310.1	95.907	562	6	2	70	614	142	703	0.0	1113

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0263630	sp\|A0A072VMJ3\|CN15C_MEDTR	95.907	562	6	2	70	614	142	703	0.0	1113
Msa0263630	sp\|A0A072VMJ3\|CN15C_MEDTR	56.604	159	56	4	1	158	1	147	6.96e-37	149

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0263630	A0A396JFQ3	56.604	159	56	4	1	158	1	147	2.66e-34	149
Msa0263630	A0A396JFQ3	95.907	562	6	2	70	614	142	703	0.0	1113

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0222240	Msa0263630	0.981997	5.682018e-154	-8.615850e-47
Msa0227280	Msa0263630	0.917552	4.621802e-86	-8.615850e-47
Msa0263630	Msa0301470	0.827174	1.809970e-54	-8.615850e-47
Msa0263630	Msa0799450	0.808596	2.865574e-50	-8.615850e-47
Msa0263630	Msa0837710	0.810558	1.085944e-50	-8.615850e-47
Msa0263630	Msa1406210	0.974283	6.973995e-138	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0263630	MtrunA17_Chr2g0326871	95.907	562	6	2	70	614	142	703	0.0	1113
Msa0263630	MtrunA17_Chr2g0326871	56.604	159	56	4	1	158	1	147	5.12e-38	149
Msa0263630	MtrunA17_Chr4g0028861	81.414	495	79	1	71	552	190	684	0.0	849
Msa0263630	MtrunA17_Chr1g0181121	61.189	572	194	4	71	614	110	681	0.0	742
Msa0263630	MtrunA17_Chr2g0305771	59.408	574	203	5	71	614	114	687	0.0	684
Msa0263630	MtrunA17_Chr3g0139071	58.204	579	207	5	71	614	144	722	0.0	671
Msa0263630	MtrunA17_Chr7g0228181	54.641	571	232	5	71	614	110	680	0.0	644
Msa0263630	MtrunA17_Chr3g0125651	52.048	586	237	6	71	612	102	687	0.0	611
Msa0263630	MtrunA17_Chr1g0158861	53.390	590	234	8	57	614	134	714	0.0	605
Msa0263630	MtrunA17_Chr3g0126541	52.922	599	245	9	46	614	120	711	0.0	605
Msa0263630	MtrunA17_Chr4g0073941	53.968	567	234	7	71	614	166	728	0.0	602
Msa0263630	MtrunA17_Chr4g0001481	53.310	574	234	9	70	612	167	737	0.0	580
Msa0263630	MtrunA17_Chr7g0275581	52.930	546	228	8	70	593	149	687	0.0	545
Msa0263630	MtrunA17_Chr8g0347121	47.333	600	256	7	70	611	126	723	3.98e-177	520
Msa0263630	MtrunA17_Chr2g0326861	94.203	207	12	0	79	285	14	220	8.31e-141	408
Msa0263630	MtrunA17_Chr8g0360821	37.087	515	271	16	71	548	129	627	2.15e-99	317
Msa0263630	MtrunA17_Chr2g0318561	36.885	488	265	10	120	574	2	479	4.14e-99	311
Msa0263630	MtrunA17_Chr6g0481021	37.911	517	282	10	68	553	187	695	1.53e-96	310
Msa0263630	MtrunA17_Chr6g0480851	37.132	509	286	11	71	553	251	751	2.72e-88	290
Msa0263630	MtrunA17_Chr7g0218341	34.857	525	287	14	73	559	179	686	3.64e-88	288
Msa0263630	MtrunA17_Chr6g0480781	35.560	509	294	12	71	553	236	736	1.15e-85	283
Msa0263630	MtrunA17_Chr6g0480671	37.859	523	275	13	71	559	260	766	8.37e-85	281
Msa0263630	MtrunA17_Chr2g0326851	90.076	131	9	1	488	614	3	133	1.63e-77	242
Msa0263630	MtrunA17_Chr6g0480741	33.075	517	302	16	68	553	179	682	3.82e-66	229
Msa0263630	MtrunA17_Chr6g0480681	30.345	580	321	18	71	591	181	736	1.84e-61	217
Msa0263630	MtrunA17_Chr5g0395421	33.244	373	203	13	180	526	3	355	1.19e-42	157
Msa0263630	MtrunA17_Chr6g0480771	35.573	253	138	8	309	547	12	253	8.89e-34	130
Msa0263630	MtrunA17_Chr5g0395411	44.444	135	73	2	70	203	225	358	7.47e-30	121
Msa0263630	MtrunA17_Chr6g0480841	40.404	198	98	7	363	547	2	192	9.14e-30	117
Msa0263630	MtrunA17_Chr6g0480821	27.211	147	101	3	121	264	25	168	1.94e-12	67.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0263630	AT2G28260.1	72.581	558	128	3	70	614	133	678	0.0	837
Msa0263630	AT2G28260.1	41.584	101	42	4	1	101	1	84	6.03e-11	65.9
Msa0263630	AT2G28260.2	72.581	558	128	3	70	614	93	638	0.0	835
Msa0263630	AT4G30360.1	56.747	578	216	5	71	614	140	717	0.0	659
Msa0263630	AT2G24610.1	56.947	583	210	6	71	612	141	723	0.0	654
Msa0263630	AT2G24610.2	56.947	583	210	6	71	612	156	738	0.0	652
Msa0263630	AT4G30560.1	55.810	568	220	8	71	614	170	730	0.0	625
Msa0263630	AT4G30560.4	55.810	568	220	8	71	614	170	730	0.0	625
Msa0263630	AT4G30560.5	55.810	568	220	8	71	614	144	704	0.0	625
Msa0263630	AT4G30560.2	55.810	568	220	8	71	614	144	704	0.0	625
Msa0263630	AT4G30560.3	55.810	568	220	8	71	614	144	704	0.0	625
Msa0263630	AT2G23980.9	54.671	578	226	6	71	614	170	745	0.0	624
Msa0263630	AT2G23980.10	54.671	578	226	6	71	614	170	745	0.0	624
Msa0263630	AT2G23980.1	54.671	578	226	6	71	614	170	745	0.0	624
Msa0263630	AT2G23980.4	54.671	578	226	6	71	614	170	745	0.0	624
Msa0263630	AT2G23980.5	54.671	578	226	6	71	614	170	745	0.0	624
Msa0263630	AT2G23980.6	54.671	578	226	6	71	614	143	718	0.0	624
Msa0263630	AT5G57940.3	54.225	568	231	5	71	614	148	710	0.0	613
Msa0263630	AT5G14870.1	50.924	595	239	7	71	612	106	700	0.0	613
Msa0263630	AT5G57940.1	54.225	568	231	5	71	614	155	717	0.0	613
Msa0263630	AT5G57940.2	54.225	568	231	5	71	614	155	717	0.0	613
Msa0263630	AT2G23980.3	52.579	601	226	7	71	614	170	768	0.0	610
Msa0263630	AT2G23980.2	52.579	601	226	7	71	614	170	768	0.0	610
Msa0263630	AT2G23980.8	52.579	601	226	7	71	614	170	768	0.0	610
Msa0263630	AT2G23980.7	52.579	601	226	7	71	614	170	768	0.0	610
Msa0263630	AT1G15990.1	53.663	587	225	8	70	614	157	738	0.0	605
Msa0263630	AT5G53130.1	54.466	571	227	5	71	614	151	715	0.0	602
Msa0263630	AT1G19780.1	53.516	583	229	8	70	611	163	744	0.0	602
Msa0263630	AT3G48010.1	55.731	506	202	4	71	554	111	616	0.0	586
Msa0263630	AT1G01340.2	50.947	581	235	9	71	612	135	704	0.0	583
Msa0263630	AT1G01340.1	50.947	581	235	9	71	612	130	699	0.0	582
Msa0263630	AT4G01010.1	50.083	601	267	9	37	612	100	692	0.0	577
Msa0263630	AT4G01010.2	50.083	601	267	9	37	612	100	692	0.0	577
Msa0263630	AT2G46430.1	50.088	569	250	9	71	611	139	701	0.0	548
Msa0263630	AT2G46430.2	50.088	569	250	9	71	611	139	701	0.0	548
Msa0263630	AT1G19780.2	52.908	533	209	8	120	611	2	533	0.0	538
Msa0263630	AT2G46440.1	44.886	528	261	11	77	582	102	621	3.81e-133	403
Msa0263630	AT2G46440.2	46.756	447	213	8	157	582	1	443	4.92e-120	363
Msa0263630	AT2G46450.3	39.564	551	289	14	67	591	52	584	1.14e-119	368
Msa0263630	AT2G46450.2	39.564	551	289	14	67	591	65	597	1.31e-119	369
Msa0263630	AT2G46450.4	39.851	537	283	13	77	591	102	620	2.90e-118	366
Msa0263630	AT2G46450.1	39.851	537	283	13	77	591	102	620	2.90e-118	366
Msa0263630	AT5G54250.2	37.347	490	263	13	97	554	177	654	1.07e-95	308
Msa0263630	AT5G54250.1	37.347	490	263	13	97	554	177	654	1.07e-95	308
Msa0263630	AT5G54250.4	37.347	490	263	13	97	554	177	654	1.07e-95	308
Msa0263630	AT3G17690.1	38.933	506	267	16	71	548	225	716	2.42e-95	308
Msa0263630	AT3G17700.1	40.080	499	260	14	71	542	259	745	4.67e-91	298
Msa0263630	AT5G54250.3	36.777	484	257	14	97	548	177	643	2.31e-90	294
Msa0263630	AT5G15410.2	36.111	504	278	12	73	542	183	676	1.80e-86	285
Msa0263630	AT5G15410.1	35.968	506	280	12	71	542	182	677	2.18e-86	285
Msa0263630	AT3G17690.2	39.336	422	223	12	71	473	225	632	6.33e-83	273
Msa0263630	AT3G17700.2	40.566	424	225	11	71	479	259	670	2.22e-80	268
Msa0263630	AT3G17690.3	38.462	299	158	9	71	350	225	516	2.99e-50	182

Find 125 sgRNAs with CRISPR-Local

Find 253 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGAAGAGAAATTTGATTCTT+TGG	0.173457	2_2:-63194033	Msa0263630:five_prime_UTR
GGAATATTTCTCACTTTAAT+TGG	0.178020	2_2:-63194324	Msa0263630:five_prime_UTR
GTAAATATAATCAATATAAA+TGG	0.208829	2_2:-63191321	Msa0263630:CDS
GTTTGGAGGGTGCAGTTCTT+TGG	0.210884	2_2:-63191864	Msa0263630:intron
AAAGGTGTTAATATGCATTC+TGG	0.262434	2_2:-63190266	Msa0263630:CDS
GAGAAATCAAATTGTCAATA+TGG	0.294895	2_2:-63192227	Msa0263630:CDS
CGAATAGACTCTCGTAGTTC+TGG	0.297309	2_2:+63191343	None:intergenic
TATTATTGCAGGTGTTGATT+TGG	0.298468	2_2:-63192974	Msa0263630:intron
TTTGGAGGGTGCAGTTCTTT+GGG	0.302499	2_2:-63191863	Msa0263630:intron
AAGGACTTCCGCTGGATCTT+AGG	0.302671	2_2:-63191261	Msa0263630:CDS
ATTCTTTGGGAAAACCAGTA+TGG	0.304135	2_2:-63194019	Msa0263630:exon
ATTGTGCAATTTGTTTGTTT+TGG	0.304710	2_2:-63190156	Msa0263630:three_prime_UTR
CACACTTGGTCTTGGATCTA+AGG	0.318439	2_2:+63190622	None:intergenic
GAAGAGAAATTTGATTCTTT+GGG	0.321524	2_2:-63194032	Msa0263630:five_prime_UTR
CCTATTCTAAGATTGCTCTT+AGG	0.322226	2_2:-63193389	Msa0263630:CDS
CTAGCATTCTTTCATCCATT+TGG	0.323336	2_2:+63190846	None:intergenic
GAAGCACACCTTTCAGATCT+TGG	0.324891	2_2:+63193985	None:intergenic
CTTGACGTTCTATTGATAGA+AGG	0.346761	2_2:+63192279	None:intergenic
GCTATTGTGGTTGCAACTCT+TGG	0.355052	2_2:-63191797	Msa0263630:CDS
GAAATGCTCTTCATAATTAG+AGG	0.358622	2_2:-63190746	Msa0263630:CDS
TGCTGCATTGCCTCTTCCTC+AGG	0.378993	2_2:-63193334	Msa0263630:intron
TTCATATATAAATTAGAGAT+AGG	0.385858	2_2:-63190125	Msa0263630:three_prime_UTR
TGGATTATCAAATCCCATAC+TGG	0.390216	2_2:+63194005	None:intergenic
CAAGGGTTGCAAGGAATACT+AGG	0.391426	2_2:-63190289	Msa0263630:CDS
GGTTCCCTTGTTTGACCAAA+TGG	0.396986	2_2:-63190861	Msa0263630:CDS
ATTCACACCAATGGAGAACT+TGG	0.401526	2_2:-63190460	Msa0263630:CDS
ATGAAGAGCATTTCGTTCAC+TGG	0.405862	2_2:+63190755	None:intergenic
GATTATTATACCAACACTTA+GGG	0.406775	2_2:-63192952	Msa0263630:CDS
TCCCACAACAAGTGGAGTTA+AGG	0.407478	2_2:-63193829	Msa0263630:CDS
GGATTATTATACCAACACTT+AGG	0.409310	2_2:-63192953	Msa0263630:CDS
AACGGATAAAAGCCGGCATT+TGG	0.410069	2_2:+63192125	None:intergenic
TCACAAATTGTAAAAGCTAC+AGG	0.415804	2_2:-63192840	Msa0263630:CDS
CCTAAGAGCAATCTTAGAAT+AGG	0.417424	2_2:+63193389	None:intergenic
CACCTTAACTCCACTTGTTG+TGG	0.420551	2_2:+63193827	None:intergenic
CTTGATTCATACACAACAAA+TGG	0.422331	2_2:-63190719	Msa0263630:CDS
GGGAGTATCACACTTGGTCT+TGG	0.424283	2_2:+63190614	None:intergenic
TTTACAATTTGTGATGAAAG+TGG	0.430401	2_2:+63192849	None:intergenic
ACCTTAACTCCACTTGTTGT+GGG	0.432233	2_2:+63193828	None:intergenic
TTGGAAAAGTGTGTGCAATA+TGG	0.436003	2_2:-63192249	Msa0263630:CDS
TTAACTGTTCTTGTGGAAGA+AGG	0.448046	2_2:+63190593	None:intergenic
CTTTGTCTTGAACTAGTTCG+AGG	0.455073	2_2:-63191220	Msa0263630:CDS
CATGGAGAAGGCACAAGAAA+AGG	0.459490	2_2:-63190415	Msa0263630:CDS
GGAAAGAAGCTGCTGAATTG+AGG	0.459874	2_2:-63190394	Msa0263630:CDS
AAGGTGTTAATATGCATTCT+GGG	0.461316	2_2:-63190265	Msa0263630:CDS
TGTGGTTGCAACTCTTGGAT+TGG	0.461815	2_2:-63191792	Msa0263630:CDS
GAAAGAAGCTGCTGAATTGA+GGG	0.466076	2_2:-63190393	Msa0263630:CDS
ACTATGCATGATGCACTTTG+TGG	0.470656	2_2:-63190067	Msa0263630:three_prime_UTR
TAAATATAATCAATATAAAT+GGG	0.474688	2_2:-63191320	Msa0263630:CDS
AAGGTGAAATACCACATTGA+TGG	0.478709	2_2:-63193810	Msa0263630:CDS
CAACCTCATGCTTTACATGT+TGG	0.485217	2_2:-63192781	Msa0263630:CDS
TTCTTCAACTCATGTAGAAT+CGG	0.487881	2_2:-63190683	Msa0263630:CDS
AGAAATAATGGTTGCTATTG+TGG	0.489273	2_2:-63191810	Msa0263630:CDS
TTCATCCATTTGGTCAAACA+AGG	0.490363	2_2:+63190856	None:intergenic
CTCATAGGTACCTGAGGAAG+AGG	0.499692	2_2:+63193324	None:intergenic
TAGAAGTACTTATTGAAGAA+TGG	0.503317	2_2:+63192059	None:intergenic
GACGAACGGTTATCGATTGA+AGG	0.504792	2_2:+63191428	None:intergenic
TTATGTTGCTCCTTCTTCGA+AGG	0.509627	2_2:-63193439	Msa0263630:CDS
TTCTTTGGGAAAACCAGTAT+GGG	0.512800	2_2:-63194018	Msa0263630:exon
GAATCTTGTGGCAGCTTCAG+AGG	0.513529	2_2:-63190363	Msa0263630:CDS
GCACTTTGTGGATGATGTCT+TGG	0.518977	2_2:-63190055	None:intergenic
TTGTGATATACTTACATGGC+TGG	0.519386	2_2:+63192758	None:intergenic
AGCTGCTGAATTGAGGGCTA+AGG	0.522860	2_2:-63190387	Msa0263630:CDS
CACAAATTGTAAAAGCTACA+GGG	0.525537	2_2:-63192839	Msa0263630:CDS
TTATTCAAGCTGCATGGAGA+AGG	0.526489	2_2:-63190427	Msa0263630:CDS
TAACTGTTCTTGTGGAAGAA+GGG	0.531786	2_2:+63190594	None:intergenic
GGGTGCAGTTCTTTGGGACA+AGG	0.533291	2_2:-63191857	Msa0263630:intron
AACTCATGTAGAATCGGTCC+AGG	0.535190	2_2:-63190677	Msa0263630:CDS
AATTTATGTTCACCAAATGC+CGG	0.535721	2_2:-63192137	Msa0263630:CDS
GAAAGCTTTAACTGTTCTTG+TGG	0.538709	2_2:+63190586	None:intergenic
TGCTCTTAGGTACTTCAGCA+AGG	0.549675	2_2:-63193376	Msa0263630:CDS
TGATGTAACTTCGGAAGTCA+TGG	0.553176	2_2:+63192085	None:intergenic
TCTTCTCAAAGGACTTCCGC+TGG	0.553705	2_2:-63191269	Msa0263630:CDS
TATACCAAACGGATAAAAGC+CGG	0.557769	2_2:+63192118	None:intergenic
TTTATGGAACAAGGGTTGCA+AGG	0.560809	2_2:-63190298	Msa0263630:CDS
TCTTCACCACAGAAATCGCC+TGG	0.562215	2_2:+63190659	None:intergenic
ATTGCTGATTGGTAATATGC+AGG	0.564527	2_2:-63191759	Msa0263630:intron
TTCACACCAATGGAGAACTT+GGG	0.569483	2_2:-63190459	Msa0263630:CDS
GCATCAGCATATATACCAAA+CGG	0.570370	2_2:+63192107	None:intergenic
AAGCAGCCCAAGTTCTCCAT+TGG	0.571876	2_2:+63190453	None:intergenic
GTTCCCTCAGTGCATAATGC+AGG	0.571915	2_2:+63190800	None:intergenic
ACAACAAATGGAGGCCGTGA+CGG	0.574379	2_2:-63190707	Msa0263630:CDS
GAGGAAGAAGATCTTCTCAA+AGG	0.575171	2_2:-63191280	Msa0263630:CDS
TAGAACGTCAAGAAGCATGT+TGG	0.581133	2_2:-63192268	Msa0263630:CDS
GCTCTTAGGTACTTCAGCAA+GGG	0.584716	2_2:-63193375	Msa0263630:CDS
GAATGGCGATGATGTAACTT+CGG	0.588257	2_2:+63192076	None:intergenic
GGGTTGTTACAGAAACAGCA+TGG	0.594816	2_2:-63192818	Msa0263630:CDS
GTTACAGAAACAGCATGGGC+AGG	0.595665	2_2:-63192813	Msa0263630:CDS
GCTATAAACCAAGCTACTCT+CGG	0.598310	2_2:+63192173	None:intergenic
GGTGGCTACACGAGGAGTAG+AGG	0.598943	2_2:-63191299	Msa0263630:CDS
GGAACTTATCTTGTTCGCGA+AGG	0.606182	2_2:-63190782	Msa0263630:CDS
TTGTTGTGTATGAATCAAGA+TGG	0.607110	2_2:+63190723	None:intergenic
GTGGTGAAGAGCTCTTAACA+TGG	0.607722	2_2:-63190646	Msa0263630:CDS
TAACCGTTCGTCTAGAAGAG+TGG	0.612435	2_2:-63191417	Msa0263630:CDS
AAGATGCTCCGAGAGTAGCT+TGG	0.612832	2_2:-63192181	Msa0263630:CDS
CCTCCACTCTTCTAGACGAA+CGG	0.613285	2_2:+63191414	None:intergenic
TCATCCATTTGGTCAAACAA+GGG	0.614441	2_2:+63190857	None:intergenic
TTGATAATCCAAGATCTGAA+AGG	0.616294	2_2:-63193993	Msa0263630:intron
GACTACATAGTAAACAACTT+AGG	0.617133	2_2:-63190499	Msa0263630:CDS
AAGCCTGCATTATGCACTGA+GGG	0.618536	2_2:-63190803	Msa0263630:CDS
AGTCTCAAAAGAATGCTACA+AGG	0.630706	2_2:-63193758	Msa0263630:CDS
CGTTCGTCTAGAAGAGTGGA+GGG	0.630762	2_2:-63191413	Msa0263630:CDS
AACACTTAGGGGTTCAACCA+TGG	0.634663	2_2:-63192940	Msa0263630:CDS
CCGTTCGTCTAGAAGAGTGG+AGG	0.639971	2_2:-63191414	Msa0263630:CDS
AAAGAACTGACACAGAACAA+TGG	0.642598	2_2:-63191387	Msa0263630:CDS
ATAGACTCTCGTAGTTCTGG+AGG	0.643923	2_2:+63191346	None:intergenic
ATCGGTCCAGGCGATTTCTG+TGG	0.645701	2_2:-63190665	Msa0263630:CDS
GATTCATACACAACAAATGG+AGG	0.646565	2_2:-63190716	Msa0263630:CDS
ATATAATCAATATAAATGGG+TGG	0.651864	2_2:-63191317	Msa0263630:CDS
GAGGGCTAAGGAGAATCTTG+TGG	0.656336	2_2:-63190375	Msa0263630:CDS
ATTATTATACCAACACTTAG+GGG	0.657353	2_2:-63192951	Msa0263630:CDS
TGGTGAAGAGCTCTTAACAT+GGG	0.661791	2_2:-63190645	Msa0263630:CDS
CTCGAACTAGTTCAAGACAA+AGG	0.662676	2_2:+63191221	None:intergenic
GGTTGTTACAGAAACAGCAT+GGG	0.662855	2_2:-63192817	Msa0263630:CDS
ACAAATTGTAAAAGCTACAG+GGG	0.665070	2_2:-63192838	Msa0263630:CDS
ATTTCTCTCCTAAGATCCAG+CGG	0.666530	2_2:+63191253	None:intergenic
GTTGCAAGGAATACTAGGAA+AGG	0.669215	2_2:-63190284	Msa0263630:CDS
ATGGTTGAACCCCTAAGTGT+TGG	0.671733	2_2:+63192942	None:intergenic
GAAGAAGGGAGTATCACACT+TGG	0.672182	2_2:+63190608	None:intergenic
AGGTGTTAATATGCATTCTG+GGG	0.675385	2_2:-63190264	Msa0263630:CDS
TATAAATGGGTGGCTACACG+AGG	0.677991	2_2:-63191307	Msa0263630:CDS
ATGAGTTGAAGAATCCGTCA+CGG	0.683468	2_2:+63190693	None:intergenic
GTATTTGTGTTCCATCAATG+TGG	0.691831	2_2:+63193799	None:intergenic
GAACTAGTTCAAGACAAAGG+TGG	0.711572	2_2:+63191224	None:intergenic
TGGCCAACATGTAAAGCATG+AGG	0.724789	2_2:+63192778	None:intergenic
AAAGCCTGCATTATGCACTG+AGG	0.736004	2_2:-63190804	Msa0263630:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	GATATTTTTATAAGATATTA+TGG	+	chr2_2:63191843-63191862	None:intergenic	10.0%
!!	GTAAATATAATCAATATAAA+TGG	-	chr2_2:63193063-63193082	Msa0263630:intron	10.0%
!!	ATATATATTCCAACAAAAAA+GGG	+	chr2_2:63190094-63190113	None:intergenic	15.0%
!!!	CTTTTCATTTTATTTTTCAT+AGG	-	chr2_2:63190503-63190522	Msa0263630:CDS	15.0%
!!	TCTGTTTATTTATGTTTAAA+AGG	-	chr2_2:63191083-63191102	Msa0263630:intron	15.0%
!!!	TTATATACTGAATAGATTTT+AGG	-	chr2_2:63192070-63192089	Msa0263630:CDS	15.0%
!!	CTTATAAACAAATATAAACT+TGG	+	chr2_2:63193206-63193225	None:intergenic	15.0%
!!	CTAATTTATTAATTGTTAAC+AGG	-	chr2_2:63193502-63193521	Msa0263630:intron	15.0%
!!	AATGAAATAGCAAAAAAATA+TGG	-	chr2_2:63194049-63194068	Msa0263630:five_prime_UTR	15.0%
!!	ATGAAATAGCAAAAAAATAT+GGG	-	chr2_2:63194050-63194069	Msa0263630:five_prime_UTR	15.0%
!!	TTCATATATAAATTAGAGAT+AGG	-	chr2_2:63194259-63194278	Msa0263630:intron	15.0%
!!	CATATATATTCCAACAAAAA+AGG	+	chr2_2:63190095-63190114	None:intergenic	20.0%
!!	AAAAATTGGTTAATGTATTG+TGG	+	chr2_2:63190303-63190322	None:intergenic	20.0%
!!	TCTAGATAACAAGAAAAAAT+TGG	+	chr2_2:63190317-63190336	None:intergenic	20.0%
!!!	GAATTTTGAAAATTATTCGA+GGG	+	chr2_2:63190432-63190451	None:intergenic	20.0%
!!	CTAATTAGTACTAAATAACA+TGG	+	chr2_2:63190454-63190473	None:intergenic	20.0%
!!!	TTAAGGATATTTCGTAATTT+TGG	+	chr2_2:63191160-63191179	None:intergenic	20.0%
!!	GTTATTTATTCTATTATTGC+AGG	-	chr2_2:63191399-63191418	Msa0263630:CDS	20.0%
!!!	TCATTTTTCAATATATTCCT+AGG	-	chr2_2:63191497-63191516	Msa0263630:intron	20.0%
!!	AAATATAATGTATTCACTCT+TGG	-	chr2_2:63192446-63192465	Msa0263630:intron	20.0%
!!	ATATAATCAATATAAATGGG+TGG	-	chr2_2:63193067-63193086	Msa0263630:intron	20.0%
!!	TATTATTCATCAGAAAAATC+AGG	+	chr2_2:63194175-63194194	None:intergenic	20.0%
!!!	ATTAAACACAGTTTTTCAAA+TGG	+	chr2_2:63194296-63194315	None:intergenic	20.0%
!	GGAATATTTCTCACTTTAAT+TGG	-	chr2_2:63190060-63190079	Msa0263630:three_prime_UTR	25.0%
!	AGAAGAAAAAGCAACAAAAT+TGG	+	chr2_2:63190125-63190144	None:intergenic	25.0%
!	ACACATTCTTGTTCAAATAT+CGG	-	chr2_2:63190212-63190231	Msa0263630:exon	25.0%
!	TGAAGAGAAATTTGATTCTT+TGG	-	chr2_2:63190351-63190370	Msa0263630:CDS	25.0%
!	GAAGAGAAATTTGATTCTTT+GGG	-	chr2_2:63190352-63190371	Msa0263630:CDS	25.0%
!	AAAATTATTCGAGGGAATTT+AGG	+	chr2_2:63190424-63190443	None:intergenic	25.0%
!!!	GGAATTTTGAAAATTATTCG+AGG	+	chr2_2:63190433-63190452	None:intergenic	25.0%
!!	ATGTTTAGTTTCTTTGTTTG+TGG	-	chr2_2:63190780-63190799	Msa0263630:CDS	25.0%
!	ACATAAATAAACAGACTCAT+AGG	+	chr2_2:63191078-63191097	None:intergenic	25.0%
!	TAGTACTTTGATAGACTAAA+CGG	+	chr2_2:63191264-63191283	None:intergenic	25.0%
!	GATTATTATACCAACACTTA+GGG	-	chr2_2:63191432-63191451	Msa0263630:CDS	25.0%
!	ATTATTATACCAACACTTAG+GGG	-	chr2_2:63191433-63191452	Msa0263630:CDS	25.0%
!	GAAAAATGATGAAAAAACGA+AGG	+	chr2_2:63191486-63191505	None:intergenic	25.0%
!	TTTACAATTTGTGATGAAAG+TGG	+	chr2_2:63191538-63191557	None:intergenic	25.0%
!	CATGCTACAAAATAGTAAAA+GGG	+	chr2_2:63191685-63191704	None:intergenic	25.0%
!	AGACTATTTCAAAGAGTTTA+CGG	-	chr2_2:63191788-63191807	Msa0263630:CDS	25.0%
!!!	TTAACTTGCTTATCCAAATT+TGG	-	chr2_2:63191968-63191987	Msa0263630:intron	25.0%
!	GAGAAATCAAATTGTCAATA+TGG	-	chr2_2:63192157-63192176	Msa0263630:CDS	25.0%
!!!	TAGAAGTACTTATTGAAGAA+TGG	+	chr2_2:63192328-63192347	None:intergenic	25.0%
!	ATAATCAACTAGATAAAGCA+AGG	+	chr2_2:63192410-63192429	None:intergenic	25.0%
!	GCTAAAAAATGGATATAATG+AGG	-	chr2_2:63192471-63192490	Msa0263630:intron	25.0%
!	AGTATTAATACCTTTCTGTT+TGG	-	chr2_2:63192503-63192522	Msa0263630:intron	25.0%
!!	TTTTCTCATACGATTCAATT+TGG	+	chr2_2:63192890-63192909	None:intergenic	25.0%
!!	TTCAAGTTATATTAGAGGTA+TGG	+	chr2_2:63192923-63192942	None:intergenic	25.0%
!!	CGAAATTCAAGTTATATTAG+AGG	+	chr2_2:63192928-63192947	None:intergenic	25.0%
!	AATATATCAAAGATCGCTAT+GGG	+	chr2_2:63193243-63193262	None:intergenic	25.0%
!	AAAAAAAGATATTCAGTCAG+AGG	+	chr2_2:63193431-63193450	None:intergenic	25.0%
!	ATAGCAAAAAAATATGGGAA+AGG	-	chr2_2:63194055-63194074	Msa0263630:intron	25.0%
!!!	TTTTGTTGTTTATGGAACAA+GGG	-	chr2_2:63194078-63194097	Msa0263630:intron	25.0%
!!!	ATTGTGCAATTTGTTTGTTT+TGG	-	chr2_2:63194228-63194247	Msa0263630:intron	25.0%
	ATATTCCAACAAAAAAGGGA+AGG	+	chr2_2:63190090-63190109	None:intergenic	30.0%
	GTTACCATCATTGATATTTG+TGG	-	chr2_2:63190277-63190296	Msa0263630:CDS	30.0%
!	TTGATAATCCAAGATCTGAA+AGG	-	chr2_2:63190391-63190410	Msa0263630:CDS	30.0%
!	GTGGGAATTTTTGATATTTC+AGG	+	chr2_2:63190541-63190560	None:intergenic	30.0%
!!!	AGCATTCTTTTGAGACTTTT+TGG	+	chr2_2:63190623-63190642	None:intergenic	30.0%
	AGTGTTAGTATTAGATCCTA+GGG	-	chr2_2:63190717-63190736	Msa0263630:CDS	30.0%
!	GAAGAAGTTTGCATTGATAT+TGG	-	chr2_2:63190835-63190854	Msa0263630:CDS	30.0%
!	TTATGTTTAAAAGGCCGTTT+TGG	-	chr2_2:63191092-63191111	Msa0263630:intron	30.0%
!!!	TTCGTAATTTTGGCTAACTT+AGG	+	chr2_2:63191150-63191169	None:intergenic	30.0%
!!	ATAACTAGAGACGCTTTTTA+AGG	+	chr2_2:63191177-63191196	None:intergenic	30.0%
	TAAACGATCAAACTATCTAG+CGG	+	chr2_2:63191224-63191243	None:intergenic	30.0%
!!	TATTATTGCAGGTGTTGATT+TGG	-	chr2_2:63191410-63191429	Msa0263630:CDS	30.0%
	GGATTATTATACCAACACTT+AGG	-	chr2_2:63191431-63191450	Msa0263630:CDS	30.0%
	TCACAAATTGTAAAAGCTAC+AGG	-	chr2_2:63191544-63191563	Msa0263630:intron	30.0%
	CACAAATTGTAAAAGCTACA+GGG	-	chr2_2:63191545-63191564	Msa0263630:intron	30.0%
	ACAAATTGTAAAAGCTACAG+GGG	-	chr2_2:63191546-63191565	Msa0263630:intron	30.0%
!	TAGCTTGTGATATACTTACA+TGG	+	chr2_2:63191633-63191652	None:intergenic	30.0%
	TATCACAAGCTATATTTCCT+TGG	-	chr2_2:63191641-63191660	Msa0263630:intron	30.0%
	GCATGCTACAAAATAGTAAA+AGG	+	chr2_2:63191686-63191705	None:intergenic	30.0%
!	TGTATTAGCTTATTCAAGCT+TGG	-	chr2_2:63191744-63191763	Msa0263630:intron	30.0%
	AAGATATTATGGAGAGCTTA+TGG	+	chr2_2:63191832-63191851	None:intergenic	30.0%
!!	ATTCTAGTTTAGGCCAAATT+TGG	+	chr2_2:63191984-63192003	None:intergenic	30.0%
	AATTTATGTTCACCAAATGC+CGG	-	chr2_2:63192247-63192266	Msa0263630:CDS	30.0%
	ATAAGTACTTCTATTGTCTC+TGG	-	chr2_2:63192335-63192354	Msa0263630:intron	30.0%
	ATTAATACCTTTCTGTTTGG+AGG	-	chr2_2:63192506-63192525	Msa0263630:intron	30.0%
	TTAATACCTTTCTGTTTGGA+GGG	-	chr2_2:63192507-63192526	Msa0263630:intron	30.0%
!	CTTCTTACTAGCACTTTTAT+CGG	-	chr2_2:63192551-63192570	Msa0263630:intron	30.0%
!	CACTTTTATCGGAGAAATAA+TGG	-	chr2_2:63192562-63192581	Msa0263630:intron	30.0%
!	AGAAATAATGGTTGCTATTG+TGG	-	chr2_2:63192574-63192593	Msa0263630:intron	30.0%
!	CGTGGTTAATTTATTTCTCT+TGG	-	chr2_2:63192652-63192671	Msa0263630:intron	30.0%
	AGAGAAATCATTTCTTACGT+TGG	-	chr2_2:63192679-63192698	Msa0263630:intron	30.0%
	TTCTTACGTTGGATGAAATA+TGG	-	chr2_2:63192690-63192709	Msa0263630:intron	30.0%
!!	ACCTTTCAAACCAGTTTTAT+AGG	-	chr2_2:63192748-63192767	Msa0263630:intron	30.0%
!!	CCTTTCAAACCAGTTTTATA+GGG	-	chr2_2:63192749-63192768	Msa0263630:intron	30.0%
	CTAATTCAACCCTATAAAAC+TGG	+	chr2_2:63192761-63192780	None:intergenic	30.0%
	TCAAAAGCAGATATAAACGA+AGG	+	chr2_2:63192826-63192845	None:intergenic	30.0%
	ATATATCAAAGATCGCTATG+GGG	+	chr2_2:63193242-63193261	None:intergenic	30.0%
	GAATATATCAAAGATCGCTA+TGG	+	chr2_2:63193244-63193263	None:intergenic	30.0%
	GAATTTGACTGTGATTTCTA+TGG	+	chr2_2:63193266-63193285	None:intergenic	30.0%
	GAAATGCTCTTCATAATTAG+AGG	-	chr2_2:63193638-63193657	Msa0263630:intron	30.0%
	TTGTTGTGTATGAATCAAGA+TGG	+	chr2_2:63193664-63193683	None:intergenic	30.0%
	CTTGATTCATACACAACAAA+TGG	-	chr2_2:63193665-63193684	Msa0263630:intron	30.0%
	TTCTTCAACTCATGTAGAAT+CGG	-	chr2_2:63193701-63193720	Msa0263630:CDS	30.0%
	GACTACATAGTAAACAACTT+AGG	-	chr2_2:63193885-63193904	Msa0263630:intron	30.0%
!	TCAGATTTTATTCACACCAA+TGG	-	chr2_2:63193915-63193934	Msa0263630:intron	30.0%
!!!	GTTTTGTTGTTTATGGAACA+AGG	-	chr2_2:63194077-63194096	Msa0263630:intron	30.0%
!!	AAAGGTGTTAATATGCATTC+TGG	-	chr2_2:63194118-63194137	Msa0263630:intron	30.0%
!	AAGGTGTTAATATGCATTCT+GGG	-	chr2_2:63194119-63194138	Msa0263630:intron	30.0%
!	ATTCTTTGGGAAAACCAGTA+TGG	-	chr2_2:63190365-63190384	Msa0263630:CDS	35.0%
!	TTCTTTGGGAAAACCAGTAT+GGG	-	chr2_2:63190366-63190385	Msa0263630:CDS	35.0%
	TGGATTATCAAATCCCATAC+TGG	+	chr2_2:63190382-63190401	None:intergenic	35.0%
	TCAAAAATTCCCACAACAAG+TGG	-	chr2_2:63190547-63190566	Msa0263630:CDS	35.0%
	AAGGTGAAATACCACATTGA+TGG	-	chr2_2:63190574-63190593	Msa0263630:CDS	35.0%
	GTATTTGTGTTCCATCAATG+TGG	+	chr2_2:63190588-63190607	None:intergenic	35.0%
!!!	GACTTTTTGGAACTTTGTTC+AGG	+	chr2_2:63190610-63190629	None:intergenic	35.0%
	AGTCTCAAAAGAATGCTACA+AGG	-	chr2_2:63190626-63190645	Msa0263630:CDS	35.0%
	GAGTGTTAGTATTAGATCCT+AGG	-	chr2_2:63190716-63190735	Msa0263630:CDS	35.0%
	GTGTTAGTATTAGATCCTAG+GGG	-	chr2_2:63190718-63190737	Msa0263630:CDS	35.0%
	ACTGGCAAATAAAAGAACAG+TGG	+	chr2_2:63190808-63190827	None:intergenic	35.0%
!!	ACTGTTCTTTTATTTGCCAG+TGG	-	chr2_2:63190807-63190826	Msa0263630:CDS	35.0%
	CTCACAGTTGTTAGATCATT+TGG	-	chr2_2:63190877-63190896	Msa0263630:intron	35.0%
	CCTAAGAGCAATCTTAGAAT+AGG	+	chr2_2:63190998-63191017	None:intergenic	35.0%
!!	CCTATTCTAAGATTGCTCTT+AGG	-	chr2_2:63190995-63191014	Msa0263630:intron	35.0%
	GCGTCTCTAGTTATTTAGTT+TGG	-	chr2_2:63191183-63191202	Msa0263630:intron	35.0%
	ACTAACAAATGATACGCTCA+AGG	-	chr2_2:63191280-63191299	Msa0263630:CDS	35.0%
	CTAACAAATGATACGCTCAA+GGG	-	chr2_2:63191281-63191300	Msa0263630:CDS	35.0%
!	GACATTTTTCGTATTTGCCA+TGG	+	chr2_2:63191464-63191483	None:intergenic	35.0%
	GGAAAAATCAAATACAGCCT+AGG	+	chr2_2:63191517-63191536	None:intergenic	35.0%
	TTGTGATATACTTACATGGC+TGG	+	chr2_2:63191629-63191648	None:intergenic	35.0%
!	CCAATTTGTTTGAACAACCA+AGG	+	chr2_2:63191661-63191680	None:intergenic	35.0%
	CCTTGGTTGTTCAAACAAAT+TGG	-	chr2_2:63191658-63191677	Msa0263630:intron	35.0%
	ATTTGGCCTAAACTAGAATG+TGG	-	chr2_2:63191985-63192004	Msa0263630:intron	35.0%
!!	CTCATTCCACATTCTAGTTT+AGG	+	chr2_2:63191994-63192013	None:intergenic	35.0%
!!	AATAGATTTTAGGAGCTTGC+TGG	-	chr2_2:63192080-63192099	Msa0263630:CDS	35.0%
	CTTGACGTTCTATTGATAGA+AGG	+	chr2_2:63192108-63192127	None:intergenic	35.0%
!	TTGGAAAAGTGTGTGCAATA+TGG	-	chr2_2:63192135-63192154	Msa0263630:CDS	35.0%
	TATACCAAACGGATAAAAGC+CGG	+	chr2_2:63192269-63192288	None:intergenic	35.0%
	GCATCAGCATATATACCAAA+CGG	+	chr2_2:63192280-63192299	None:intergenic	35.0%
	TTAACCATAATACTTGCCTC+AGG	+	chr2_2:63192375-63192394	None:intergenic	35.0%
	CACTCTTGGAAGCTAAAAAA+TGG	-	chr2_2:63192460-63192479	Msa0263630:intron	35.0%
!	ATTGCTGATTGGTAATATGC+AGG	-	chr2_2:63192625-63192644	Msa0263630:intron	35.0%
!!	CCCTATAAAACTGGTTTGAA+AGG	+	chr2_2:63192752-63192771	None:intergenic	35.0%
!	GGGTTGAATTAGTTTGAAGA+TGG	-	chr2_2:63192769-63192788	Msa0263630:intron	35.0%
	AAAGAACTGACACAGAACAA+TGG	-	chr2_2:63192997-63193016	Msa0263630:intron	35.0%
	TCATCCATTTGGTCAAACAA+GGG	+	chr2_2:63193530-63193549	None:intergenic	35.0%
	TTCATCCATTTGGTCAAACA+AGG	+	chr2_2:63193531-63193550	None:intergenic	35.0%
	CTAGCATTCTTTCATCCATT+TGG	+	chr2_2:63193541-63193560	None:intergenic	35.0%
	GATTCATACACAACAAATGG+AGG	-	chr2_2:63193668-63193687	Msa0263630:intron	35.0%
	TAACTGTTCTTGTGGAAGAA+GGG	+	chr2_2:63193793-63193812	None:intergenic	35.0%
	TTAACTGTTCTTGTGGAAGA+AGG	+	chr2_2:63193794-63193813	None:intergenic	35.0%
!	GAAAGCTTTAACTGTTCTTG+TGG	+	chr2_2:63193801-63193820	None:intergenic	35.0%
!!!	CTTGTTTTATTCAAGCTGCA+TGG	-	chr2_2:63193951-63193970	Msa0263630:intron	35.0%
!!!	GGGAAAGGTTTTGTTGTTTA+TGG	-	chr2_2:63194070-63194089	Msa0263630:intron	35.0%
	AGGTGTTAATATGCATTCTG+GGG	-	chr2_2:63194120-63194139	Msa0263630:intron	35.0%
	TCAGAAAAATCAGGTTCTGT+TGG	+	chr2_2:63194166-63194185	None:intergenic	35.0%
	GTGGCCACAAATATCAATGA+TGG	+	chr2_2:63190284-63190303	None:intergenic	40.0%
!!	ACCTTAACTCCACTTGTTGT+GGG	+	chr2_2:63190559-63190578	None:intergenic	40.0%
	CAAACTTCTTCTCTAACCAC+TGG	+	chr2_2:63190826-63190845	None:intergenic	40.0%
	TTATGTTGCTCCTTCTTCGA+AGG	-	chr2_2:63190945-63190964	Msa0263630:intron	40.0%
	CTACCAAAAACCTTCGAAGA+AGG	+	chr2_2:63190958-63190977	None:intergenic	40.0%
!!!	TCTTCGAAGGTTTTTGGTAG+AGG	-	chr2_2:63190958-63190977	Msa0263630:intron	40.0%
	AGCACATTCAAGGACCAAAA+CGG	+	chr2_2:63191109-63191128	None:intergenic	40.0%
	AGTGACACTGTCGTACATTT+AGG	-	chr2_2:63191337-63191356	Msa0263630:CDS	40.0%
	GGTTGTTACAGAAACAGCAT+GGG	-	chr2_2:63191567-63191586	Msa0263630:intron	40.0%
	CAACCTCATGCTTTACATGT+TGG	-	chr2_2:63191603-63191622	Msa0263630:intron	40.0%
!	TAGAACGTCAAGAAGCATGT+TGG	-	chr2_2:63192116-63192135	Msa0263630:CDS	40.0%
	GCTATAAACCAAGCTACTCT+CGG	+	chr2_2:63192214-63192233	None:intergenic	40.0%
	TGATGTAACTTCGGAAGTCA+TGG	+	chr2_2:63192302-63192321	None:intergenic	40.0%
	GAATGGCGATGATGTAACTT+CGG	+	chr2_2:63192311-63192330	None:intergenic	40.0%
	AGTACTTCTATTGTCTCTGG+TGG	-	chr2_2:63192338-63192357	Msa0263630:intron	40.0%
	GTACTTCTATTGTCTCTGGT+GGG	-	chr2_2:63192339-63192358	Msa0263630:intron	40.0%
	TACTTCTATTGTCTCTGGTG+GGG	-	chr2_2:63192340-63192359	Msa0263630:intron	40.0%
!	GTACTTTTCGCATTGCTGAT+TGG	-	chr2_2:63192614-63192633	Msa0263630:intron	40.0%
	CGAAGGATGTATGTGTTTCA+CGG	+	chr2_2:63192809-63192828	None:intergenic	40.0%
	GAGGAAGAAGATCTTCTCAA+AGG	-	chr2_2:63193104-63193123	Msa0263630:intron	40.0%
	ATTTCTCTCCTAAGATCCAG+CGG	+	chr2_2:63193134-63193153	None:intergenic	40.0%
	GAACTAGTTCAAGACAAAGG+TGG	+	chr2_2:63193163-63193182	None:intergenic	40.0%
	CTCGAACTAGTTCAAGACAA+AGG	+	chr2_2:63193166-63193185	None:intergenic	40.0%
	CTTTGTCTTGAACTAGTTCG+AGG	-	chr2_2:63193164-63193183	Msa0263630:intron	40.0%
	TGATCTGGATCACAACATCA+AGG	+	chr2_2:63193330-63193349	None:intergenic	40.0%
	AAAGATCTGCGACTATGATC+TGG	+	chr2_2:63193345-63193364	None:intergenic	40.0%
!!	ATGAAGAGCATTTCGTTCAC+TGG	+	chr2_2:63193632-63193651	None:intergenic	40.0%
!	ATGAGTTGAAGAATCCGTCA+CGG	+	chr2_2:63193694-63193713	None:intergenic	40.0%
	TGGTGAAGAGCTCTTAACAT+GGG	-	chr2_2:63193739-63193758	Msa0263630:CDS	40.0%
	ATTCACACCAATGGAGAACT+TGG	-	chr2_2:63193924-63193943	Msa0263630:intron	40.0%
	TTCACACCAATGGAGAACTT+GGG	-	chr2_2:63193925-63193944	Msa0263630:intron	40.0%
	TTATTCAAGCTGCATGGAGA+AGG	-	chr2_2:63193957-63193976	Msa0263630:intron	40.0%
	GAAAGAAGCTGCTGAATTGA+GGG	-	chr2_2:63193991-63194010	Msa0263630:intron	40.0%
	TTTATGGAACAAGGGTTGCA+AGG	-	chr2_2:63194086-63194105	Msa0263630:intron	40.0%
	GTTGCAAGGAATACTAGGAA+AGG	-	chr2_2:63194100-63194119	Msa0263630:intron	40.0%
	ACTATGCATGATGCACTTTG+TGG	-	chr2_2:63194317-63194336	Msa0263630:five_prime_UTR	40.0%
!!!	GTGCTCCTTCCCTTTTTTGT+TGG	-	chr2_2:63190082-63190101	Msa0263630:three_prime_UTR	45.0%
!	TCAATGATGGTAACGCGCAA+AGG	+	chr2_2:63190271-63190290	None:intergenic	45.0%
	GAAGCACACCTTTCAGATCT+TGG	+	chr2_2:63190402-63190421	None:intergenic	45.0%
	TCCCACAACAAGTGGAGTTA+AGG	-	chr2_2:63190555-63190574	Msa0263630:CDS	45.0%
!	CACCTTAACTCCACTTGTTG+TGG	+	chr2_2:63190560-63190579	None:intergenic	45.0%
!	GCTCCTTCTTCGAAGGTTTT+TGG	-	chr2_2:63190952-63190971	Msa0263630:intron	45.0%
	TGCTCTTAGGTACTTCAGCA+AGG	-	chr2_2:63191008-63191027	Msa0263630:intron	45.0%
	GCTCTTAGGTACTTCAGCAA+GGG	-	chr2_2:63191009-63191028	Msa0263630:intron	45.0%
!	GGTACTTCAGCAAGGGTTTT+TGG	-	chr2_2:63191016-63191035	Msa0263630:intron	45.0%
	AACAGACTCATAGGTACCTG+AGG	+	chr2_2:63191069-63191088	None:intergenic	45.0%
	GACGACATCTAGCACATTCA+AGG	+	chr2_2:63191119-63191138	None:intergenic	45.0%
	ATGGTTGAACCCCTAAGTGT+TGG	+	chr2_2:63191445-63191464	None:intergenic	45.0%
	AACACTTAGGGGTTCAACCA+TGG	-	chr2_2:63191444-63191463	Msa0263630:intron	45.0%
	GGGTTGTTACAGAAACAGCA+TGG	-	chr2_2:63191566-63191585	Msa0263630:intron	45.0%
	TGGCCAACATGTAAAGCATG+AGG	+	chr2_2:63191609-63191628	None:intergenic	45.0%
	AACGGATAAAAGCCGGCATT+TGG	+	chr2_2:63192262-63192281	None:intergenic	45.0%
!!!	AATGCCGGCTTTTATCCGTT+TGG	-	chr2_2:63192262-63192281	Msa0263630:CDS	45.0%
	ATTGTCTCTGGTGGGGATTA+AGG	-	chr2_2:63192347-63192366	Msa0263630:intron	45.0%
	GGAACCTGAGGCAAGTATTA+TGG	-	chr2_2:63192368-63192387	Msa0263630:intron	45.0%
!	TTTGGAGGGTGCAGTTCTTT+GGG	-	chr2_2:63192521-63192540	Msa0263630:intron	45.0%
	GCTATTGTGGTTGCAACTCT+TGG	-	chr2_2:63192587-63192606	Msa0263630:intron	45.0%
	TGTGGTTGCAACTCTTGGAT+TGG	-	chr2_2:63192592-63192611	Msa0263630:intron	45.0%
	TGGTAATATGCAGGTAAGCG+TGG	-	chr2_2:63192634-63192653	Msa0263630:intron	45.0%
!	GACGAACGGTTATCGATTGA+AGG	+	chr2_2:63192959-63192978	None:intergenic	45.0%
	TAACCGTTCGTCTAGAAGAG+TGG	-	chr2_2:63192967-63192986	Msa0263630:intron	45.0%
!!	ATAGACTCTCGTAGTTCTGG+AGG	+	chr2_2:63193041-63193060	None:intergenic	45.0%
	CGAATAGACTCTCGTAGTTC+TGG	+	chr2_2:63193044-63193063	None:intergenic	45.0%
	TATAAATGGGTGGCTACACG+AGG	-	chr2_2:63193077-63193096	Msa0263630:intron	45.0%
!	GGTTCCCTTGTTTGACCAAA+TGG	-	chr2_2:63193523-63193542	Msa0263630:intron	45.0%
	AAAGCCTGCATTATGCACTG+AGG	-	chr2_2:63193580-63193599	Msa0263630:intron	45.0%
	AAGCCTGCATTATGCACTGA+GGG	-	chr2_2:63193581-63193600	Msa0263630:intron	45.0%
	GGAACTTATCTTGTTCGCGA+AGG	-	chr2_2:63193602-63193621	Msa0263630:intron	45.0%
	AACTCATGTAGAATCGGTCC+AGG	-	chr2_2:63193707-63193726	Msa0263630:CDS	45.0%
	GTGGTGAAGAGCTCTTAACA+TGG	-	chr2_2:63193738-63193757	Msa0263630:CDS	45.0%
!!	CACACTTGGTCTTGGATCTA+AGG	+	chr2_2:63193765-63193784	None:intergenic	45.0%
!!	GAAGAAGGGAGTATCACACT+TGG	+	chr2_2:63193779-63193798	None:intergenic	45.0%
!	CATGGAGAAGGCACAAGAAA+AGG	-	chr2_2:63193969-63193988	Msa0263630:intron	45.0%
	GGAAAGAAGCTGCTGAATTG+AGG	-	chr2_2:63193990-63194009	Msa0263630:intron	45.0%
	CAAGGGTTGCAAGGAATACT+AGG	-	chr2_2:63194095-63194114	Msa0263630:intron	45.0%
!!	TAAATATAATCAATATAAAT+GGG	-	chr2_2:63193064-63193083	Msa0263630:intron	5.0%
	CGATGTATGAGTTGTCCCCT+AGG	+	chr2_2:63190736-63190755	None:intergenic	50.0%
	GGGGACAACTCATACATCGT+TGG	-	chr2_2:63190737-63190756	Msa0263630:CDS	50.0%
	CTCATAGGTACCTGAGGAAG+AGG	+	chr2_2:63191063-63191082	None:intergenic	50.0%
	GTTACAGAAACAGCATGGGC+AGG	-	chr2_2:63191571-63191590	Msa0263630:intron	50.0%
	AAGATGCTCCGAGAGTAGCT+TGG	-	chr2_2:63192203-63192222	Msa0263630:CDS	50.0%
	ACTGCACCCTCCAAACAGAA+AGG	+	chr2_2:63192516-63192535	None:intergenic	50.0%
!	GTTTGGAGGGTGCAGTTCTT+TGG	-	chr2_2:63192520-63192539	Msa0263630:intron	50.0%
	CCTCCACTCTTCTAGACGAA+CGG	+	chr2_2:63192973-63192992	None:intergenic	50.0%
	CGTTCGTCTAGAAGAGTGGA+GGG	-	chr2_2:63192971-63192990	Msa0263630:intron	50.0%
	TCTTCTCAAAGGACTTCCGC+TGG	-	chr2_2:63193115-63193134	Msa0263630:intron	50.0%
	AAGGACTTCCGCTGGATCTT+AGG	-	chr2_2:63193123-63193142	Msa0263630:intron	50.0%
	AGCTGAAGCAGCCACAATCA+CGG	-	chr2_2:63193286-63193305	Msa0263630:intron	50.0%
	TGTCTCCACAACCGTGATTG+TGG	+	chr2_2:63193300-63193319	None:intergenic	50.0%
	GTTCCCTCAGTGCATAATGC+AGG	+	chr2_2:63193587-63193606	None:intergenic	50.0%
	ACAACAAATGGAGGCCGTGA+CGG	-	chr2_2:63193677-63193696	Msa0263630:intron	50.0%
	TCTTCACCACAGAAATCGCC+TGG	+	chr2_2:63193728-63193747	None:intergenic	50.0%
!!	GGGAGTATCACACTTGGTCT+TGG	+	chr2_2:63193773-63193792	None:intergenic	50.0%
	AAGCAGCCCAAGTTCTCCAT+TGG	+	chr2_2:63193934-63193953	None:intergenic	50.0%
	AGCTGCTGAATTGAGGGCTA+AGG	-	chr2_2:63193997-63194016	Msa0263630:CDS	50.0%
	GAGGGCTAAGGAGAATCTTG+TGG	-	chr2_2:63194009-63194028	Msa0263630:exon	50.0%
	GAATCTTGTGGCAGCTTCAG+AGG	-	chr2_2:63194021-63194040	Msa0263630:exon	50.0%
!	TGGTAACGCGCAAAGGCGAT+TGG	+	chr2_2:63190264-63190283	None:intergenic	55.0%
	TGCTGCATTGCCTCTTCCTC+AGG	-	chr2_2:63191050-63191069	Msa0263630:intron	55.0%
	GGTGGGGATTAAGGAACCTG+AGG	-	chr2_2:63192356-63192375	Msa0263630:intron	55.0%
!!	GGGTGCAGTTCTTTGGGACA+AGG	-	chr2_2:63192527-63192546	Msa0263630:intron	55.0%
	CCGTTCGTCTAGAAGAGTGG+AGG	-	chr2_2:63192970-63192989	Msa0263630:intron	55.0%
	AGCAGCCACAATCACGGTTG+TGG	-	chr2_2:63193292-63193311	Msa0263630:intron	55.0%
	ATCGGTCCAGGCGATTTCTG+TGG	-	chr2_2:63193719-63193738	Msa0263630:CDS	55.0%
	GGTGGCTACACGAGGAGTAG+AGG	-	chr2_2:63193085-63193104	Msa0263630:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2_2	gene	63190057	63194349	63190057	ID=Msa0263630;Name=Msa0263630
chr2_2	mRNA	63190057	63194349	63190057	ID=Msa0263630-mRNA-1;Parent=Msa0263630;Name=Msa0263630-mRNA-1;_AED=0.10;_eAED=0.10;_QI=105\|0.75\|0.66\|1\|0.87\|0.77\|9\|157\|614
chr2_2	exon	63190057	63190882	63190057	ID=Msa0263630-mRNA-1:exon:19633;Parent=Msa0263630-mRNA-1
chr2_2	exon	63191219	63191455	63191219	ID=Msa0263630-mRNA-1:exon:19632;Parent=Msa0263630-mRNA-1
chr2_2	exon	63191760	63191871	63191760	ID=Msa0263630-mRNA-1:exon:19631;Parent=Msa0263630-mRNA-1
chr2_2	exon	63192068	63192321	63192068	ID=Msa0263630-mRNA-1:exon:19630;Parent=Msa0263630-mRNA-1
chr2_2	exon	63192773	63192985	63192773	ID=Msa0263630-mRNA-1:exon:19629;Parent=Msa0263630-mRNA-1
chr2_2	exon	63193335	63193484	63193335	ID=Msa0263630-mRNA-1:exon:19628;Parent=Msa0263630-mRNA-1
chr2_2	exon	63193701	63193881	63193701	ID=Msa0263630-mRNA-1:exon:19627;Parent=Msa0263630-mRNA-1
chr2_2	exon	63193994	63194070	63193994	ID=Msa0263630-mRNA-1:exon:19626;Parent=Msa0263630-mRNA-1
chr2_2	exon	63194293	63194349	63194293	ID=Msa0263630-mRNA-1:exon:19625;Parent=Msa0263630-mRNA-1
chr2_2	five_prime_UTR	63194293	63194349	63194293	ID=Msa0263630-mRNA-1:five_prime_utr;Parent=Msa0263630-mRNA-1
chr2_2	five_prime_UTR	63194023	63194070	63194023	ID=Msa0263630-mRNA-1:five_prime_utr;Parent=Msa0263630-mRNA-1
chr2_2	CDS	63193994	63194022	63193994	ID=Msa0263630-mRNA-1:cds;Parent=Msa0263630-mRNA-1
chr2_2	CDS	63193701	63193881	63193701	ID=Msa0263630-mRNA-1:cds;Parent=Msa0263630-mRNA-1
chr2_2	CDS	63193335	63193484	63193335	ID=Msa0263630-mRNA-1:cds;Parent=Msa0263630-mRNA-1
chr2_2	CDS	63192773	63192985	63192773	ID=Msa0263630-mRNA-1:cds;Parent=Msa0263630-mRNA-1
chr2_2	CDS	63192068	63192321	63192068	ID=Msa0263630-mRNA-1:cds;Parent=Msa0263630-mRNA-1
chr2_2	CDS	63191760	63191871	63191760	ID=Msa0263630-mRNA-1:cds;Parent=Msa0263630-mRNA-1
chr2_2	CDS	63191219	63191455	63191219	ID=Msa0263630-mRNA-1:cds;Parent=Msa0263630-mRNA-1
chr2_2	CDS	63190214	63190882	63190214	ID=Msa0263630-mRNA-1:cds;Parent=Msa0263630-mRNA-1
chr2_2	three_prime_UTR	63190057	63190213	63190057	ID=Msa0263630-mRNA-1:three_prime_utr;Parent=Msa0263630-mRNA-1

Gene Sequence

>Msa0263630

ATGGGATTTGATAATCCAAGATCTGAAAGGTTTGAAGATGATCCTGAAATATCAAAAATTCCCACAACAAGTGGAGTTAAGGTGAAATACCACATTGATGGAACACAAATACCTGAACAAAGTTCCAAAAAGTCTCAAAAGAATGCTACAAGGAACAAGTTTTTGAAAACAAGAGTATTGTCAAGAGTATTCTCAGAAGACTATGAAAGAATTTGTATGAAGTTTCGTACAGCTTATGTTGCTCCTTCTTCGAAGGTTTTTGGTAGAGGAGAACTTGTTCTTACCTATTCTAAGATTGCTCTTAGGTACTTCAGCAAGGGTTTTTGGTTAGACTCTATTGCTGCATTGCCTCTTCCTCAGGTGTTGATTTGGATTATTATACCAACACTTAGGGGTTCAACCATGGCAAATACGAAAAATGTCCTTCGTTTTTTCATCATTTTTCAATATATTCCTAGGCTGTATTTGATTTTTCCACTTTCATCACAAATTGTAAAAGCTACAGGGGTTGTTACAGAAACAGCATGGGCAGGTGCTGCTTACAACCTCATGCTTTACATGTTGGCCAGCCATATTTTAGGAGCTTGCTGGTACCTTCTATCAATAGAACGTCAAGAAGCATGTTGGAAAAGTGTGTGCAATATGGAGAAATCAAATTGTCAATATGGATTCTTCAATTGTCACAGTGTTAAAGATGCTCCGAGAGTAGCTTGGTTTATAGCTAGTAATGTCACAAATTTATGTTCACCAAATGCCGGCTTTTATCCGTTTGGTATATATGCTGATGCCATGACTTCCGAAGTTACATCATCGCCATTCTTCAATAATTCTTTGGGACAAGGTCTTCTTACTAGCACTTTTATCGGAGAAATAATGGTTGCTATTGTGGTTGCAACTCTTGGATTGGTACTTTTCGCATTGCTGATTGGTAATATGCAGACATACCTTCAATCGATAACCGTTCGTCTAGAAGAGTGGAGGGTGAAAAGAACTGACACAGAACAATGGATGCATCACAGACAACTACCTCCAGAACTACGAGAGTCTATTCGTAAATATAATCAATATAAATGGGTGGCTACACGAGGAGTAGAGGAAGAAGATCTTCTCAAAGGACTTCCGCTGGATCTTAGGAGAGAAATCAAACGCCACCTTTGTCTTGAACTAGTTCGAGGAGTTCCCTTGTTTGACCAAATGGATGAAAGAATGCTAGACGCAATATGCGAAAGACTAAAGCCTGCATTATGCACTGAGGGAACTTATCTTGTTCGCGAAGGCGATCCAGTGAACGAAATGCTCTTCATAATTAGAGGCCATCTTGATTCATACACAACAAATGGAGGCCGTGACGGATTCTTCAACTCATGTAGAATCGGTCCAGGCGATTTCTGTGGTGAAGAGCTCTTAACATGGGCCTTAGATCCAAGACCAAGTGTGATACTCCCTTCTTCCACAAGAACAGTTAAAGCTTTCTCAGAAGTAGAAGCTTTTGCACTTATAGCTGAAGATCTTAAGTTTGTTGCATCTCAATTTAGAAGACTACATAGTAAACAACTTAGGCATAAATTCAGATTTTATTCACACCAATGGAGAACTTGGGCTGCTTGTTTTATTCAAGCTGCATGGAGAAGGCACAAGAAAAGGAAAGAAGCTGCTGAATTGAGGGCTAAGGAGAATCTTGTGGCAGCTTCAGAGGCTGAAAATGAAATAGCAAAAAAATATGGGAAAGGTTTTGTTGTTTATGGAACAAGGGTTGCAAGGAATACTAGGAAAGGTGTTAATATGCATTCTGGGGTAGTAAGCTCTTTGCAGAAACCAACAGAACCTGATTTTTCTGATGAATAA

Protein sequence

>Msa0263630

MGFDNPRSERFEDDPEISKIPTTSGVKVKYHIDGTQIPEQSSKKSQKNATRNKFLKTRVLSRVFSEDYERICMKFRTAYVAPSSKVFGRGELVLTYSKIALRYFSKGFWLDSIAALPLPQVLIWIIIPTLRGSTMANTKNVLRFFIIFQYIPRLYLIFPLSSQIVKATGVVTETAWAGAAYNLMLYMLASHILGACWYLLSIERQEACWKSVCNMEKSNCQYGFFNCHSVKDAPRVAWFIASNVTNLCSPNAGFYPFGIYADAMTSEVTSSPFFNNSLGQGLLTSTFIGEIMVAIVVATLGLVLFALLIGNMQTYLQSITVRLEEWRVKRTDTEQWMHHRQLPPELRESIRKYNQYKWVATRGVEEEDLLKGLPLDLRREIKRHLCLELVRGVPLFDQMDERMLDAICERLKPALCTEGTYLVREGDPVNEMLFIIRGHLDSYTTNGGRDGFFNSCRIGPGDFCGEELLTWALDPRPSVILPSSTRTVKAFSEVEAFALIAEDLKFVASQFRRLHSKQLRHKFRFYSHQWRTWAACFIQAAWRRHKKRKEAAELRAKENLVAASEAENEIAKKYGKGFVVYGTRVARNTRKGVNMHSGVVSSLQKPTEPDFSDE*