AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0301470

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0301470 MtrunA17_Chr2g0326871 96.728 703 10 1 1 690 1 703 0.0 1407
Msa0301470 MtrunA17_Chr4g0028861 76.596 611 129 2 28 624 74 684 0.0 983
Msa0301470 MtrunA17_Chr1g0181121 60.386 674 241 4 41 690 10 681 0.0 855
Msa0301470 MtrunA17_Chr2g0305771 54.793 699 260 10 28 690 9 687 0.0 761
Msa0301470 MtrunA17_Chr3g0139071 54.339 703 273 9 30 690 26 722 0.0 738
Msa0301470 MtrunA17_Chr7g0228181 53.271 642 273 5 74 690 41 680 0.0 716
Msa0301470 MtrunA17_Chr3g0126541 49.517 725 317 11 1 690 1 711 0.0 683
Msa0301470 MtrunA17_Chr4g0073941 50.363 689 311 10 27 690 46 728 0.0 679
Msa0301470 MtrunA17_Chr3g0125651 50.613 652 282 5 77 688 36 687 0.0 679
Msa0301470 MtrunA17_Chr1g0158861 52.262 641 270 8 77 690 83 714 0.0 669
Msa0301470 MtrunA17_Chr4g0001481 50.755 662 293 12 55 688 81 737 0.0 634
Msa0301470 MtrunA17_Chr7g0275581 50.702 641 282 10 77 690 83 716 0.0 620
Msa0301470 MtrunA17_Chr8g0347121 46.765 680 301 9 65 687 48 723 0.0 595
Msa0301470 MtrunA17_Chr2g0326861 93.720 207 13 0 151 357 14 220 5.07e-139 406
Msa0301470 MtrunA17_Chr8g0360821 37.177 581 311 17 78 620 63 627 7.96e-118 367
Msa0301470 MtrunA17_Chr6g0481021 37.694 581 322 11 77 625 123 695 1.49e-114 360
Msa0301470 MtrunA17_Chr6g0480851 37.500 576 325 12 77 625 184 751 4.47e-110 350
Msa0301470 MtrunA17_Chr6g0480671 37.966 590 315 14 77 631 193 766 2.63e-106 340
Msa0301470 MtrunA17_Chr6g0480781 35.417 576 337 12 77 625 169 736 1.63e-105 338
Msa0301470 MtrunA17_Chr7g0218341 32.625 659 392 17 12 631 41 686 3.48e-98 317
Msa0301470 MtrunA17_Chr2g0318561 36.885 488 265 10 192 646 2 479 4.41e-98 310
Msa0301470 MtrunA17_Chr6g0480741 33.448 580 343 16 77 625 115 682 3.65e-86 285
Msa0301470 MtrunA17_Chr2g0326851 93.130 131 9 0 560 690 3 133 1.97e-82 256
Msa0301470 MtrunA17_Chr6g0480681 30.960 646 363 18 77 663 115 736 4.24e-80 270
Msa0301470 MtrunA17_Chr5g0395421 33.244 373 203 13 252 598 3 355 1.92e-42 158
Msa0301470 MtrunA17_Chr5g0395411 38.500 200 109 3 77 275 172 358 1.61e-41 155
Msa0301470 MtrunA17_Chr6g0480771 35.573 253 138 8 381 619 12 253 1.54e-33 130
Msa0301470 MtrunA17_Chr6g0480841 40.404 198 98 7 435 619 2 192 1.25e-29 117
Msa0301470 MtrunA17_Chr6g0480791 39.516 124 73 2 77 198 172 295 3.93e-23 100
Msa0301470 MtrunA17_Chr6g0480751 34.091 132 86 1 77 207 98 229 2.40e-21 93.6
Msa0301470 MtrunA17_Chr2g0318551 32.317 164 97 4 76 237 62 213 4.27e-15 75.1
Msa0301470 MtrunA17_Chr6g0480821 31.126 151 90 5 193 336 25 168 1.60e-14 73.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0301470 AT2G28260.1 69.844 703 174 6 1 690 1 678 0.0 993
Msa0301470 AT2G28260.2 72.018 654 152 4 50 690 3 638 0.0 964
Msa0301470 AT4G30360.1 54.321 648 261 6 77 690 71 717 0.0 721
Msa0301470 AT2G24610.1 54.478 670 257 8 62 688 59 723 0.0 716
Msa0301470 AT2G24610.2 54.328 670 258 8 62 688 74 738 0.0 714
Msa0301470 AT4G30560.1 53.385 650 271 9 63 690 91 730 0.0 686
Msa0301470 AT4G30560.4 53.385 650 271 9 63 690 91 730 0.0 686
Msa0301470 AT2G23980.6 51.672 658 285 6 63 690 64 718 0.0 686
Msa0301470 AT2G23980.9 51.672 658 285 6 63 690 91 745 0.0 685
Msa0301470 AT2G23980.10 51.672 658 285 6 63 690 91 745 0.0 685
Msa0301470 AT2G23980.1 51.672 658 285 6 63 690 91 745 0.0 685
Msa0301470 AT2G23980.4 51.672 658 285 6 63 690 91 745 0.0 685
Msa0301470 AT2G23980.5 51.672 658 285 6 63 690 91 745 0.0 685
Msa0301470 AT4G30560.5 53.385 650 271 9 63 690 65 704 0.0 685
Msa0301470 AT4G30560.2 53.385 650 271 9 63 690 65 704 0.0 685
Msa0301470 AT4G30560.3 53.385 650 271 9 63 690 65 704 0.0 685
Msa0301470 AT5G57940.1 51.488 672 296 7 40 690 55 717 0.0 684
Msa0301470 AT5G57940.2 51.488 672 296 7 40 690 55 717 0.0 684
Msa0301470 AT5G57940.3 51.488 672 296 7 40 690 48 710 0.0 682
Msa0301470 AT5G53130.1 49.306 720 330 8 1 690 1 715 0.0 678
Msa0301470 AT5G14870.1 49.247 664 288 7 74 688 37 700 0.0 676
Msa0301470 AT1G15990.1 48.971 729 313 12 13 690 18 738 0.0 674
Msa0301470 AT2G23980.3 49.927 681 285 7 63 690 91 768 0.0 672
Msa0301470 AT2G23980.2 49.927 681 285 7 63 690 91 768 0.0 672
Msa0301470 AT2G23980.8 49.927 681 285 7 63 690 91 768 0.0 672
Msa0301470 AT2G23980.7 49.927 681 285 7 63 690 91 768 0.0 672
Msa0301470 AT1G19780.1 50.661 681 295 9 45 687 67 744 0.0 667
Msa0301470 AT3G48010.1 54.498 578 240 5 72 626 39 616 0.0 655
Msa0301470 AT1G01340.2 50.155 646 279 10 77 688 68 704 0.0 645
Msa0301470 AT1G01340.1 50.155 646 279 10 77 688 63 699 0.0 645
Msa0301470 AT4G01010.1 50.394 635 282 8 77 688 68 692 0.0 640
Msa0301470 AT4G01010.2 50.394 635 282 8 77 688 68 692 0.0 640
Msa0301470 AT2G46430.1 49.686 636 289 10 77 687 72 701 0.0 610
Msa0301470 AT2G46430.2 49.686 636 289 10 77 687 72 701 0.0 610
Msa0301470 AT1G19780.2 52.720 533 214 7 192 687 2 533 0.0 541
Msa0301470 AT2G46440.1 42.698 630 328 13 48 654 2 621 1.12e-152 456
Msa0301470 AT2G46450.4 37.651 664 371 15 48 688 2 645 1.74e-138 421
Msa0301470 AT2G46450.1 37.651 664 371 15 48 688 2 645 1.74e-138 421
Msa0301470 AT2G46450.2 36.295 664 357 16 48 688 2 622 1.36e-127 392
Msa0301470 AT2G46450.3 36.661 641 342 15 71 688 10 609 1.01e-126 389
Msa0301470 AT2G46440.2 46.756 447 213 8 229 654 1 443 5.60e-119 363
Msa0301470 AT5G54250.2 36.942 582 317 15 77 620 79 648 6.38e-115 361
Msa0301470 AT5G54250.1 36.942 582 317 15 77 620 79 648 6.38e-115 361
Msa0301470 AT5G54250.4 36.942 582 317 15 77 620 79 648 6.38e-115 361
Msa0301470 AT3G17690.1 37.871 573 313 17 77 620 158 716 2.22e-111 353
Msa0301470 AT5G54250.3 36.254 582 316 16 77 620 79 643 1.53e-109 347
Msa0301470 AT3G17700.1 39.046 566 305 15 77 614 192 745 3.16e-107 343
Msa0301470 AT5G15410.2 36.000 575 321 14 77 614 112 676 3.19e-101 326
Msa0301470 AT5G15410.1 36.000 575 321 14 77 614 113 677 4.68e-101 325
Msa0301470 AT3G17690.2 38.037 489 269 13 77 545 158 632 5.42e-99 318
Msa0301470 AT3G17700.2 39.308 491 270 12 77 551 192 670 1.26e-96 313
Msa0301470 AT3G17690.3 36.885 366 204 10 77 422 158 516 5.05e-66 227

Find 154 sgRNAs with CRISPR-Local

Find 271 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAGAGAAATTTGATTCTT+TGG 0.173457 2_3:-57564874 Msa0301470:five_prime_UTR
GGAATATTTCTCACTTTAAT+TGG 0.178020 2_3:-57565167 Msa0301470:five_prime_UTR
ATGCATTCTGGGACAAATTC+TGG 0.206486 2_3:-57561227 Msa0301470:CDS
GTAAATATAATCAATATAAA+TGG 0.208829 2_3:-57562295 Msa0301470:CDS
CTCACAGTTGTTAGATCATT+TGG 0.229887 2_3:-57564349 Msa0301470:CDS
GAAGAAGTTTGCATTGATAT+TGG 0.251828 2_3:-57564391 Msa0301470:CDS
GTTTGGTGGGTGCAGTTCTT+TGG 0.263404 2_3:-57562833 Msa0301470:intron
GATTCTTTGGGAAACCAGTA+TGG 0.272841 2_3:-57564861 Msa0301470:exon
GTCACACTCCCCTTAAAATA+TGG 0.279845 2_3:+57565301 None:intergenic
AAAGGTGTTAATATGCATTC+TGG 0.287094 2_3:-57561239 Msa0301470:CDS
GAGAAATCAAATTGTCAATA+TGG 0.294895 2_3:-57563200 Msa0301470:CDS
CGAATAGACTCTCGTAGTTC+TGG 0.297309 2_3:+57562317 None:intergenic
TATTATTGCAGGTGTTGATT+TGG 0.298468 2_3:-57563815 Msa0301470:intron
AAGGACTTCCGCTGGATCTT+AGG 0.302671 2_3:-57562235 Msa0301470:CDS
CACACTTGGTCTTGGATCTA+AGG 0.318439 2_3:+57561595 None:intergenic
GAAGAGAAATTTGATTCTTT+GGG 0.321524 2_3:-57564873 Msa0301470:five_prime_UTR
CTAGCATTCTTTCATCCATT+TGG 0.323336 2_3:+57561819 None:intergenic
GAAACACACCTTTCAGATCT+TGG 0.324533 2_3:+57564827 None:intergenic
GTTGTGTAATTTGTTTGTTT+TGG 0.330286 2_3:-57561120 Msa0301470:three_prime_UTR
CTTGACGTTCTATTGATAGA+AGG 0.346761 2_3:+57563252 None:intergenic
CCTATTCTAAGATCGCTCTT+AGG 0.351411 2_3:-57564231 Msa0301470:CDS
TTTGGTGGGTGCAGTTCTTT+GGG 0.351931 2_3:-57562832 Msa0301470:intron
GCTATTGTGGTTGCAACTCT+TGG 0.355052 2_3:-57562766 Msa0301470:CDS
GAAATGCTCTTCATAATTAG+AGG 0.358622 2_3:-57561719 Msa0301470:CDS
TTCATATATAAATTAGATAT+AGG 0.364925 2_3:-57561089 Msa0301470:three_prime_UTR
AACAACTTAGGCATAAATTC+AGG 0.365686 2_3:-57561460 Msa0301470:CDS
ATGTTTAGTTTCTTTGTTTG+TGG 0.366840 2_3:-57564446 Msa0301470:CDS
ATTCACATCAATGGAGAACT+TGG 0.375627 2_3:-57561433 Msa0301470:CDS
TGCTGCATTGCCTCTTCCTC+AGG 0.378993 2_3:-57564176 Msa0301470:intron
TGCAGCAGAGGGAAATGCTT+TGG 0.385038 2_3:-57565188 Msa0301470:five_prime_UTR
CAAGGGTTGCAAGGAATACT+AGG 0.391426 2_3:-57561262 Msa0301470:CDS
CCAACTGGTTTCTAGTTCAT+AGG 0.394399 2_3:-57565229 Msa0301470:five_prime_UTR
GGTTCCCTTGTTTGACCAAA+TGG 0.396986 2_3:-57561834 Msa0301470:CDS
CATATATATTCCAACACAAA+AGG 0.399767 2_3:+57565135 None:intergenic
TGGATTATCAAACCCCATAC+TGG 0.404490 2_3:+57564847 None:intergenic
ATGAAGAGCATTTCGTTCAC+TGG 0.405862 2_3:+57561728 None:intergenic
GATTATTATACCAACACTTA+GGG 0.406775 2_3:-57563793 Msa0301470:CDS
TCCCACAACAAGTGGAGTTA+AGG 0.407478 2_3:-57564671 Msa0301470:CDS
GGATTATTATACCAACACTT+AGG 0.409310 2_3:-57563794 Msa0301470:CDS
TCACAAATTGTAAAAGCTAC+AGG 0.415804 2_3:-57563681 Msa0301470:CDS
TTACTATGCCATGCACTTTG+TGG 0.417427 2_3:-57561033 Msa0301470:three_prime_UTR
CTGGAAAAGTGTGTGCAATA+TGG 0.419897 2_3:-57563222 Msa0301470:CDS
CACCTTAACTCCACTTGTTG+TGG 0.420551 2_3:+57564669 None:intergenic
CTTGATTCATACACAACAAA+TGG 0.422331 2_3:-57561692 Msa0301470:CDS
TGCATTCTGGGACAAATTCT+GGG 0.423660 2_3:-57561226 Msa0301470:CDS
GGGAGTATCACACTTGGTCT+TGG 0.424283 2_3:+57561587 None:intergenic
TTTACAATTTGTGATGAAAG+TGG 0.430401 2_3:+57563690 None:intergenic
ACCTTAACTCCACTTGTTGT+GGG 0.432233 2_3:+57564670 None:intergenic
GAGTGTTAGTATTAGATCCT+AGG 0.432543 2_3:-57564510 Msa0301470:CDS
AACGGATAATAGCCGGCATT+TGG 0.443667 2_3:+57563098 None:intergenic
TTATGTTGCTCCTTCTTCCA+AGG 0.452854 2_3:-57564281 Msa0301470:CDS
GCACTTTGTGGATGATGTCT+TGG 0.454772 2_3:-57561021 None:intergenic
CTTTGTCTTGAACTAGTTCG+AGG 0.455073 2_3:-57562194 Msa0301470:CDS
CCTAAGAGCGATCTTAGAAT+AGG 0.455565 2_3:+57564231 None:intergenic
CATGGAGAAGGCACAAGAAA+AGG 0.459490 2_3:-57561388 Msa0301470:CDS
GGAAAGAAGCTGCTGAATTG+AGG 0.459874 2_3:-57561367 Msa0301470:CDS
TGTGGTTGCAACTCTTGGAT+TGG 0.461815 2_3:-57562761 Msa0301470:CDS
GAAAGAAGCTGCTGAATTGA+GGG 0.466076 2_3:-57561366 Msa0301470:CDS
TAAATATAATCAATATAAAT+GGG 0.474688 2_3:-57562294 Msa0301470:CDS
AAGGTGAAATACCACATTGA+TGG 0.478709 2_3:-57564652 Msa0301470:CDS
AATGCCGGCTATTATCCGTT+TGG 0.479271 2_3:-57563095 Msa0301470:CDS
CAACCTCATGCTTTACATGT+TGG 0.485217 2_3:-57563622 Msa0301470:CDS
TTCTTCAACTCATGTAGAAT+CGG 0.487881 2_3:-57561656 Msa0301470:CDS
AGAAATAATGGTTGCTATTG+TGG 0.489273 2_3:-57562779 Msa0301470:CDS
TTCATCCATTTGGTCAAACA+AGG 0.490363 2_3:+57561829 None:intergenic
CTCATAGGTACCTGAGGAAG+AGG 0.499692 2_3:+57564166 None:intergenic
TAGAAGTACTTATTGAAGAA+TGG 0.503317 2_3:+57563032 None:intergenic
TATACTGCAACTCCTCCAAC+TGG 0.503848 2_3:-57565244 Msa0301470:five_prime_UTR
GACGAACGGTTATCGATTGA+AGG 0.504792 2_3:+57562402 None:intergenic
TTAACTGTTCTTGTTGAGGA+AGG 0.505244 2_3:+57561566 None:intergenic
AAGGTGTTAATATGCATTCT+GGG 0.512894 2_3:-57561238 Msa0301470:CDS
GAATCTTGTGGCAGCTTCAG+AGG 0.513529 2_3:-57561336 Msa0301470:CDS
ATTCTTTGGGAAACCAGTAT+GGG 0.514755 2_3:-57564860 Msa0301470:exon
GGGTGCAGTTCTTTGGGACA+AGG 0.520447 2_3:-57562826 Msa0301470:intron
AGCTGCTGAATTGAGGGCTA+AGG 0.522860 2_3:-57561360 Msa0301470:CDS
CACAAATTGTAAAAGCTACA+GGG 0.525537 2_3:-57563680 Msa0301470:CDS
TTATTCAAGCTGCATGGAGA+AGG 0.526489 2_3:-57561400 Msa0301470:CDS
TAGTGATATACTTACATGGC+TGG 0.527731 2_3:+57563599 None:intergenic
AGTGTTAGTATTAGATCCTA+GGG 0.528702 2_3:-57564509 Msa0301470:CDS
CGCTCTTAGGTACTTCAGCA+AGG 0.534603 2_3:-57564218 Msa0301470:CDS
AACTCATGTAGAATCGGTCC+AGG 0.535190 2_3:-57561650 Msa0301470:CDS
AGCTTTAACTGTTCTTGTTG+AGG 0.541957 2_3:+57561562 None:intergenic
TAACTGTTCTTGTTGAGGAA+GGG 0.543680 2_3:+57561567 None:intergenic
TTCACATCAATGGAGAACTT+GGG 0.546433 2_3:-57561432 Msa0301470:CDS
TAGAACGTCAAGAAGCATGC+TGG 0.547890 2_3:-57563241 Msa0301470:CDS
TGATGTAACTTCGGAAGTCA+TGG 0.553176 2_3:+57563058 None:intergenic
ATATATATTCCAACACAAAA+GGG 0.553495 2_3:+57565136 None:intergenic
TCTTCTCAAAGGACTTCCGC+TGG 0.553705 2_3:-57562243 Msa0301470:CDS
CGATGTATGAGTTGTCCCCT+AGG 0.557395 2_3:+57564493 None:intergenic
TTTATGGAACAAGGGTTGCA+AGG 0.560809 2_3:-57561271 Msa0301470:CDS
TCTTCACCACAGAAATCGCC+TGG 0.562215 2_3:+57561632 None:intergenic
ATTGCTGATTGGTAATATGC+AGG 0.564527 2_3:-57562728 Msa0301470:intron
AATTTATGTTCACCAAATGC+CGG 0.564738 2_3:-57563110 Msa0301470:CDS
GGATAAGTTGCTTGCAGCAG+AGG 0.569822 2_3:-57565200 Msa0301470:five_prime_UTR
GCATCAGCATATATACCAAA+CGG 0.570370 2_3:+57563080 None:intergenic
GTTCCCTCAGTGCATAATGC+AGG 0.571915 2_3:+57561773 None:intergenic
TATACCAAACGGATAATAGC+CGG 0.574347 2_3:+57563091 None:intergenic
ACAACAAATGGAGGCCGTGA+CGG 0.574379 2_3:-57561680 Msa0301470:CDS
GAGGAAGAAGATCTTCTCAA+AGG 0.575171 2_3:-57562254 Msa0301470:CDS
GACATCATCCACAAAGTGCA+TGG 0.577403 2_3:+57561025 None:intergenic
GTGACAGAAACAGCATGGGC+AGG 0.582455 2_3:-57563654 Msa0301470:CDS
ATCTTCAGCTATAAGTGCAA+AGG 0.585973 2_3:+57561523 None:intergenic
TGGTTTCTAGTTCATAGGTG+TGG 0.586045 2_3:-57565224 Msa0301470:five_prime_UTR
GAATGGCGATGATGTAACTT+CGG 0.588257 2_3:+57563049 None:intergenic
CCTATGAACTAGAAACCAGT+TGG 0.597683 2_3:+57565229 None:intergenic
GCTCTTAGGTACTTCAGCAA+GGG 0.598148 2_3:-57564217 Msa0301470:CDS
GCTATAAACCAAGCTACTCT+CGG 0.598310 2_3:+57563146 None:intergenic
GGTGGCTACACGAGGAGTAG+AGG 0.598943 2_3:-57562273 Msa0301470:CDS
TTTCTAGTTCATAGGTGTGG+AGG 0.600735 2_3:-57565221 Msa0301470:five_prime_UTR
ATGAACTAGAAACCAGTTGG+AGG 0.603367 2_3:+57565232 None:intergenic
CAAACTTCTTCTCTAACCAC+TGG 0.605564 2_3:+57564403 None:intergenic
GGAACTTATCTTGTTCGCGA+AGG 0.606182 2_3:-57561755 Msa0301470:CDS
TTGTTGTGTATGAATCAAGA+TGG 0.607110 2_3:+57561696 None:intergenic
GTGGTGAAGAGCTCTTAACA+TGG 0.607722 2_3:-57561619 Msa0301470:CDS
GGGGACAACTCATACATCGT+TGG 0.608201 2_3:-57564489 Msa0301470:CDS
TAACCGTTCGTCTAGAAGAG+TGG 0.612435 2_3:-57562391 Msa0301470:CDS
AAGATGCTCCGAGAGTAGCT+TGG 0.612832 2_3:-57563154 Msa0301470:CDS
CCTCCACTCTTCTAGACGAA+CGG 0.613285 2_3:+57562388 None:intergenic
TCATCCATTTGGTCAAACAA+GGG 0.614441 2_3:+57561830 None:intergenic
GGGTTGTGACAGAAACAGCA+TGG 0.616045 2_3:-57563659 Msa0301470:CDS
TTGATAATCCAAGATCTGAA+AGG 0.616294 2_3:-57564835 Msa0301470:intron
GACTACATAGTAAACAACTT+AGG 0.617133 2_3:-57561472 Msa0301470:CDS
AAGCCTGCATTATGCACTGA+GGG 0.618536 2_3:-57561776 Msa0301470:CDS
GATAAGTTGCTTGCAGCAGA+GGG 0.620001 2_3:-57565199 Msa0301470:five_prime_UTR
AGTCTCAAAAGAATGCTACA+AGG 0.630706 2_3:-57564600 Msa0301470:CDS
CGTTCGTCTAGAAGAGTGGA+GGG 0.630762 2_3:-57562387 Msa0301470:CDS
AACACTTAGGGGTTCAACCA+TGG 0.634663 2_3:-57563781 Msa0301470:CDS
ATATTCCAACACAAAAGGGA+AGG 0.639138 2_3:+57565140 None:intergenic
CCGTTCGTCTAGAAGAGTGG+AGG 0.639971 2_3:-57562388 Msa0301470:CDS
AAAGAACTGACACAGAACAA+TGG 0.642598 2_3:-57562361 Msa0301470:CDS
ATAGACTCTCGTAGTTCTGG+AGG 0.643923 2_3:+57562320 None:intergenic
TTCTTTGGGAAACCAGTATG+GGG 0.643927 2_3:-57564859 Msa0301470:exon
ATCGGTCCAGGCGATTTCTG+TGG 0.645701 2_3:-57561638 Msa0301470:CDS
GATTCATACACAACAAATGG+AGG 0.646565 2_3:-57561689 Msa0301470:CDS
ATATAATCAATATAAATGGG+TGG 0.651864 2_3:-57562291 Msa0301470:CDS
GAGGGCTAAGGAGAATCTTG+TGG 0.656336 2_3:-57561348 Msa0301470:CDS
ATTATTATACCAACACTTAG+GGG 0.657353 2_3:-57563792 Msa0301470:CDS
GGTTGTGACAGAAACAGCAT+GGG 0.657973 2_3:-57563658 Msa0301470:CDS
TGGTGAAGAGCTCTTAACAT+GGG 0.661791 2_3:-57561618 Msa0301470:CDS
CTCGAACTAGTTCAAGACAA+AGG 0.662676 2_3:+57562195 None:intergenic
ACAAATTGTAAAAGCTACAG+GGG 0.665070 2_3:-57563679 Msa0301470:CDS
ATTTCTCTCCTAAGATCCAG+CGG 0.666530 2_3:+57562227 None:intergenic
GTTGCAAGGAATACTAGGAA+AGG 0.669215 2_3:-57561257 Msa0301470:CDS
ATGGTTGAACCCCTAAGTGT+TGG 0.671733 2_3:+57563783 None:intergenic
GAGGAAGGGAGTATCACACT+TGG 0.674478 2_3:+57561581 None:intergenic
TATAAATGGGTGGCTACACG+AGG 0.677991 2_3:-57562281 Msa0301470:CDS
ACTGGCAAATAAAAGAACAG+TGG 0.683258 2_3:+57564421 None:intergenic
ATGAGTTGAAGAATCCGTCA+CGG 0.683468 2_3:+57561666 None:intergenic
GCATTCTGGGACAAATTCTG+GGG 0.683525 2_3:-57561225 Msa0301470:CDS
GTATTTGTGTTCCATCAATG+TGG 0.691831 2_3:+57564641 None:intergenic
GAACTAGTTCAAGACAAAGG+TGG 0.711572 2_3:+57562198 None:intergenic
TGGCCAACATGTAAAGCATG+AGG 0.724789 2_3:+57563619 None:intergenic
AAAGCCTGCATTATGCACTG+AGG 0.736004 2_3:-57561777 Msa0301470:CDS
GTGTTAGTATTAGATCCTAG+GGG 0.750831 2_3:-57564508 Msa0301470:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTAAATATAATCAATATAAA+TGG - chr2_3:57564030-57564049 Msa0301470:intron 10.0%
!! TTCATATATAAATTAGATAT+AGG - chr2_3:57565236-57565255 Msa0301470:five_prime_UTR 10.0%
!!! CTTTTCATTTTATTTTTCAT+AGG - chr2_3:57561602-57561621 Msa0301470:CDS 15.0%
!! TCTGTTTATTTATGTTTAAA+AGG - chr2_3:57562182-57562201 Msa0301470:intron 15.0%
!!! TATTTTCATATAAGCTAAAA+GGG + chr2_3:57562781-57562800 None:intergenic 15.0%
!!! TTATATTCTGAATAGATTTT+AGG - chr2_3:57563038-57563057 Msa0301470:intron 15.0%
!! CTTATAAACAAATATAAACT+TGG + chr2_3:57564173-57564192 None:intergenic 15.0%
!!! ACTGAATATCATTTTTTTTT+TGG - chr2_3:57564401-57564420 Msa0301470:CDS 15.0%
!! AATGAAATAGCAAAAAAATA+TGG - chr2_3:57565017-57565036 Msa0301470:intron 15.0%
!! ATGAAATAGCAAAAAAATAT+GGG - chr2_3:57565018-57565037 Msa0301470:intron 15.0%
!! ATATATATTCCAACACAAAA+GGG + chr2_3:57561192-57561211 None:intergenic 20.0%
!! AAATTGCTTAATATGTATTG+TGG + chr2_3:57561401-57561420 None:intergenic 20.0%
!!! GAATTTTGAATGTTATTTGA+GGG + chr2_3:57561531-57561550 None:intergenic 20.0%
!! GTTATTTATTCTATTATTGC+AGG - chr2_3:57562499-57562518 Msa0301470:intron 20.0%
!! AATATAGTGATATACTTACA+TGG + chr2_3:57562733-57562752 None:intergenic 20.0%
!!! CTATTTTCATATAAGCTAAA+AGG + chr2_3:57562782-57562801 None:intergenic 20.0%
!!! TATAAAAGTATGAGTAAGTT+TGG + chr2_3:57563023-57563042 None:intergenic 20.0%
!! AAATATAATGTATTCACTCT+TGG - chr2_3:57563418-57563437 Msa0301470:intron 20.0%
!! ATATAATCAATATAAATGGG+TGG - chr2_3:57564034-57564053 Msa0301470:intron 20.0%
!! ATAGAAATCAAAGTCAAATT+CGG - chr2_3:57564232-57564251 Msa0301470:CDS 20.0%
!!! AAGTATTGAAACAATTTGTA+AGG - chr2_3:57564344-57564363 Msa0301470:CDS 20.0%
!! CTAACTTATTAATTGTTAAC+AGG - chr2_3:57564470-57564489 Msa0301470:CDS 20.0%
!! TATTATTCATCAGAAAAATC+AGG + chr2_3:57565155-57565174 None:intergenic 20.0%
!!! GATTTTTCTGATGAATAATA+AGG - chr2_3:57565155-57565174 Msa0301470:five_prime_UTR 20.0%
!!! ATTAAACACAGTTTTTCAAA+TGG + chr2_3:57565273-57565292 None:intergenic 20.0%
! GGAATATTTCTCACTTTAAT+TGG - chr2_3:57561158-57561177 Msa0301470:exon 25.0%
! CATATATATTCCAACACAAA+AGG + chr2_3:57561193-57561212 None:intergenic 25.0%
! AGAAGAAAAAGCAACAAAAT+TGG + chr2_3:57561223-57561242 None:intergenic 25.0%
! ACACATTCTTGTTCAAATAT+CGG - chr2_3:57561310-57561329 Msa0301470:CDS 25.0%
! TGAAGAGAAATTTGATTCTT+TGG - chr2_3:57561451-57561470 Msa0301470:CDS 25.0%
! GAAGAGAAATTTGATTCTTT+GGG - chr2_3:57561452-57561471 Msa0301470:CDS 25.0%
!!! GGAATTTTGAATGTTATTTG+AGG + chr2_3:57561532-57561551 None:intergenic 25.0%
!! ATGTTTAGTTTCTTTGTTTG+TGG - chr2_3:57561879-57561898 Msa0301470:intron 25.0%
! ACATAAATAAACAGACTCAT+AGG + chr2_3:57562177-57562196 None:intergenic 25.0%
!!! TTCGTAATTTTGGTTAACTT+GGG + chr2_3:57562249-57562268 None:intergenic 25.0%
!!! TTTCGTAATTTTGGTTAACT+TGG + chr2_3:57562250-57562269 None:intergenic 25.0%
!! TTAAGGATGTTTCGTAATTT+TGG + chr2_3:57562259-57562278 None:intergenic 25.0%
! TCATTTGTTCGTAATTAGAT+AGG + chr2_3:57562372-57562391 None:intergenic 25.0%
! TTAGAATCCTAAATGTATGA+CGG + chr2_3:57562447-57562466 None:intergenic 25.0%
! GATTATTATACCAACACTTA+GGG - chr2_3:57562532-57562551 Msa0301470:intron 25.0%
! ATTATTATACCAACACTTAG+GGG - chr2_3:57562533-57562552 Msa0301470:intron 25.0%
! GAAAAATGATGAAAAAACGA+AGG + chr2_3:57562586-57562605 None:intergenic 25.0%
!!! TCATTTTTCAATATATTCCG+AGG - chr2_3:57562597-57562616 Msa0301470:intron 25.0%
! TTTACAATTTGTGATGAAAG+TGG + chr2_3:57562638-57562657 None:intergenic 25.0%
! AGTATATCACTATATTTCCT+TGG - chr2_3:57562737-57562756 Msa0301470:CDS 25.0%
!!! ATTTTAGTTTAGGCCAAATT+TGG + chr2_3:57562904-57562923 None:intergenic 25.0%
!!! CTTATTCCACATTTTAGTTT+AGG + chr2_3:57562914-57562933 None:intergenic 25.0%
! GAGAAATCAAATTGTCAATA+TGG - chr2_3:57563125-57563144 Msa0301470:CDS 25.0%
!!! TAGAAGTACTTATTGAAGAA+TGG + chr2_3:57563296-57563315 None:intergenic 25.0%
! ATAATCAACTAGATAAAGCA+AGG + chr2_3:57563378-57563397 None:intergenic 25.0%
! GCTAAAAAATGGATATAATG+AGG - chr2_3:57563443-57563462 Msa0301470:intron 25.0%
! AGTATTAATACCTTTCTGTT+TGG - chr2_3:57563475-57563494 Msa0301470:intron 25.0%
! ATAGAAATCATTTCTTACGT+TGG - chr2_3:57563651-57563670 Msa0301470:CDS 25.0%
!! TTTTCTCATACGATTCAATT+TGG + chr2_3:57563862-57563881 None:intergenic 25.0%
!! CGAAATTCAAGTTATATTAG+AGG + chr2_3:57563895-57563914 None:intergenic 25.0%
! AATATATCAAAGATCGCTAT+GGG + chr2_3:57564210-57564229 None:intergenic 25.0%
!! GAATTTGACTTTGATTTCTA+TGG + chr2_3:57564233-57564252 None:intergenic 25.0%
! AAAAAATGATATTCAGTCAG+AGG + chr2_3:57564398-57564417 None:intergenic 25.0%
! ATAGCAAAAAAATATGGGAA+AGG - chr2_3:57565023-57565042 Msa0301470:intron 25.0%
!!! TTTTGTTGTTTATGGAACAA+GGG - chr2_3:57565046-57565065 Msa0301470:intron 25.0%
!!! GTTGTGTAATTTGTTTGTTT+TGG - chr2_3:57565205-57565224 Msa0301470:five_prime_UTR 25.0%
GTTACCATCATTGATATTTG+TGG - chr2_3:57561375-57561394 Msa0301470:CDS 30.0%
! TTGATAATCCAAGATCTGAA+AGG - chr2_3:57561490-57561509 Msa0301470:CDS 30.0%
! AATGTTATTTGAGGGAGTTT+AGG + chr2_3:57561523-57561542 None:intergenic 30.0%
CTAATCAGTACTAAATAGCT+TGG + chr2_3:57561553-57561572 None:intergenic 30.0%
! GTGGGAATTTTTGATATTTC+AGG + chr2_3:57561640-57561659 None:intergenic 30.0%
!!! AGCATTCTTTTGAGACTTTT+TGG + chr2_3:57561722-57561741 None:intergenic 30.0%
AGTGTTAGTATTAGATCCTA+GGG - chr2_3:57561816-57561835 Msa0301470:CDS 30.0%
! GAAGAAGTTTGCATTGATAT+TGG - chr2_3:57561934-57561953 Msa0301470:intron 30.0%
! TAACTAGGAGATGCATTTTA+AGG + chr2_3:57562276-57562295 None:intergenic 30.0%
CATCTCCTAGTTATTTAGTT+CGG - chr2_3:57562283-57562302 Msa0301470:CDS 30.0%
TAAACGATCAAACTAACTAG+CGG + chr2_3:57562324-57562343 None:intergenic 30.0%
!! TATTATTGCAGGTGTTGATT+TGG - chr2_3:57562510-57562529 Msa0301470:intron 30.0%
GGATTATTATACCAACACTT+AGG - chr2_3:57562531-57562550 Msa0301470:intron 30.0%
TCACAAATTGTAAAAGCTAC+AGG - chr2_3:57562644-57562663 Msa0301470:intron 30.0%
CACAAATTGTAAAAGCTACA+GGG - chr2_3:57562645-57562664 Msa0301470:intron 30.0%
ACAAATTGTAAAAGCTACAG+GGG - chr2_3:57562646-57562665 Msa0301470:intron 30.0%
TTAACCTGCTTATCCAAATT+TGG - chr2_3:57562888-57562907 Msa0301470:intron 30.0%
ATTTGGCCTAAACTAAAATG+TGG - chr2_3:57562905-57562924 Msa0301470:intron 30.0%
AGTATGAGTAAGTTTGGAAA+TGG + chr2_3:57563017-57563036 None:intergenic 30.0%
AATTTATGTTCACCAAATGC+CGG - chr2_3:57563215-57563234 Msa0301470:CDS 30.0%
ATAAGTACTTCTATTGTCTC+TGG - chr2_3:57563303-57563322 Msa0301470:intron 30.0%
ATTAATACCTTTCTGTTTGG+TGG - chr2_3:57563478-57563497 Msa0301470:intron 30.0%
TTAATACCTTTCTGTTTGGT+GGG - chr2_3:57563479-57563498 Msa0301470:intron 30.0%
! CTTCTTACTAGCACTTTTAT+CGG - chr2_3:57563523-57563542 Msa0301470:intron 30.0%
! CACTTTTATCGGAGAAATAA+TGG - chr2_3:57563534-57563553 Msa0301470:intron 30.0%
! AGAAATAATGGTTGCTATTG+TGG - chr2_3:57563546-57563565 Msa0301470:intron 30.0%
! CGTGGTTAATTTATTTCTCT+TGG - chr2_3:57563624-57563643 Msa0301470:CDS 30.0%
TTCTTACGTTGGATGAAATA+TGG - chr2_3:57563662-57563681 Msa0301470:CDS 30.0%
!! ACCTTTCAAACCAGTTTTAT+AGG - chr2_3:57563720-57563739 Msa0301470:CDS 30.0%
!! CCTTTCAAACCAGTTTTATA+GGG - chr2_3:57563721-57563740 Msa0301470:CDS 30.0%
CTAATTCAACCCTATAAAAC+TGG + chr2_3:57563733-57563752 None:intergenic 30.0%
TCAAAAGCAGATATAAACGA+AGG + chr2_3:57563798-57563817 None:intergenic 30.0%
ATATATCAAAGATCGCTATG+GGG + chr2_3:57564209-57564228 None:intergenic 30.0%
GAATATATCAAAGATCGCTA+TGG + chr2_3:57564211-57564230 None:intergenic 30.0%
GAAATGCTCTTCATAATTAG+AGG - chr2_3:57564606-57564625 Msa0301470:CDS 30.0%
TTGTTGTGTATGAATCAAGA+TGG + chr2_3:57564632-57564651 None:intergenic 30.0%
CTTGATTCATACACAACAAA+TGG - chr2_3:57564633-57564652 Msa0301470:CDS 30.0%
TTCTTCAACTCATGTAGAAT+CGG - chr2_3:57564669-57564688 Msa0301470:CDS 30.0%
GACTACATAGTAAACAACTT+AGG - chr2_3:57564853-57564872 Msa0301470:exon 30.0%
AACAACTTAGGCATAAATTC+AGG - chr2_3:57564865-57564884 Msa0301470:five_prime_UTR 30.0%
!!! TCAGGTTTTATTCACATCAA+TGG - chr2_3:57564883-57564902 Msa0301470:five_prime_UTR 30.0%
!!! GTTTTGTTGTTTATGGAACA+AGG - chr2_3:57565045-57565064 Msa0301470:intron 30.0%
!! AAAGGTGTTAATATGCATTC+TGG - chr2_3:57565086-57565105 Msa0301470:intron 30.0%
! AAGGTGTTAATATGCATTCT+GGG - chr2_3:57565087-57565106 Msa0301470:intron 30.0%
ATATTCCAACACAAAAGGGA+AGG + chr2_3:57561188-57561207 None:intergenic 35.0%
! ATTCTTTGGGAAACCAGTAT+GGG - chr2_3:57561465-57561484 Msa0301470:CDS 35.0%
TCAAAAATTCCCACAACAAG+TGG - chr2_3:57561646-57561665 Msa0301470:CDS 35.0%
AAGGTGAAATACCACATTGA+TGG - chr2_3:57561673-57561692 Msa0301470:CDS 35.0%
GTATTTGTGTTCCATCAATG+TGG + chr2_3:57561687-57561706 None:intergenic 35.0%
!!! GACTTTTTGGAACTTTGTTC+AGG + chr2_3:57561709-57561728 None:intergenic 35.0%
AGTCTCAAAAGAATGCTACA+AGG - chr2_3:57561725-57561744 Msa0301470:CDS 35.0%
GAGTGTTAGTATTAGATCCT+AGG - chr2_3:57561815-57561834 Msa0301470:CDS 35.0%
GTGTTAGTATTAGATCCTAG+GGG - chr2_3:57561817-57561836 Msa0301470:CDS 35.0%
ACTGGCAAATAAAAGAACAG+TGG + chr2_3:57561907-57561926 None:intergenic 35.0%
!! ACTGTTCTTTTATTTGCCAG+TGG - chr2_3:57561906-57561925 Msa0301470:intron 35.0%
CTCACAGTTGTTAGATCATT+TGG - chr2_3:57561976-57561995 Msa0301470:intron 35.0%
GACACATTCAAGGATCAAAA+CGG + chr2_3:57562208-57562227 None:intergenic 35.0%
GAGAACCGAACTAAATAACT+AGG + chr2_3:57562291-57562310 None:intergenic 35.0%
! GACATTTTTCGTATTTGCCA+TGG + chr2_3:57562564-57562583 None:intergenic 35.0%
GGAAAAATCAAATACAGCCT+CGG + chr2_3:57562617-57562636 None:intergenic 35.0%
TAGTGATATACTTACATGGC+TGG + chr2_3:57562729-57562748 None:intergenic 35.0%
! CCAATTTGTTTGAACAACCA+AGG + chr2_3:57562757-57562776 None:intergenic 35.0%
CCTTGGTTGTTCAAACAAAT+TGG - chr2_3:57562754-57562773 Msa0301470:CDS 35.0%
!! AATAGATTTTAGGAGCTTGC+TGG - chr2_3:57563048-57563067 Msa0301470:CDS 35.0%
CTTGACGTTCTATTGATAGA+AGG + chr2_3:57563076-57563095 None:intergenic 35.0%
TATACCAAACGGATAATAGC+CGG + chr2_3:57563237-57563256 None:intergenic 35.0%
GCATCAGCATATATACCAAA+CGG + chr2_3:57563248-57563267 None:intergenic 35.0%
TTAACCATAATACTTGCCTC+AGG + chr2_3:57563343-57563362 None:intergenic 35.0%
CACTCTTGGAAGCTAAAAAA+TGG - chr2_3:57563432-57563451 Msa0301470:intron 35.0%
! ATTGCTGATTGGTAATATGC+AGG - chr2_3:57563597-57563616 Msa0301470:intron 35.0%
!! CCCTATAAAACTGGTTTGAA+AGG + chr2_3:57563724-57563743 None:intergenic 35.0%
AAAGAACTGACACAGAACAA+TGG - chr2_3:57563964-57563983 Msa0301470:intron 35.0%
TCATCCATTTGGTCAAACAA+GGG + chr2_3:57564498-57564517 None:intergenic 35.0%
TTCATCCATTTGGTCAAACA+AGG + chr2_3:57564499-57564518 None:intergenic 35.0%
CTAGCATTCTTTCATCCATT+TGG + chr2_3:57564509-57564528 None:intergenic 35.0%
GATTCATACACAACAAATGG+AGG - chr2_3:57564636-57564655 Msa0301470:CDS 35.0%
TAACTGTTCTTGTTGAGGAA+GGG + chr2_3:57564761-57564780 None:intergenic 35.0%
TTAACTGTTCTTGTTGAGGA+AGG + chr2_3:57564762-57564781 None:intergenic 35.0%
! AGCTTTAACTGTTCTTGTTG+AGG + chr2_3:57564766-57564785 None:intergenic 35.0%
ATCTTCAGCTATAAGTGCAA+AGG + chr2_3:57564805-57564824 None:intergenic 35.0%
ATTCACATCAATGGAGAACT+TGG - chr2_3:57564892-57564911 Msa0301470:five_prime_UTR 35.0%
TTCACATCAATGGAGAACTT+GGG - chr2_3:57564893-57564912 Msa0301470:intron 35.0%
!!! CTTGTTTTATTCAAGCTGCA+TGG - chr2_3:57564919-57564938 Msa0301470:intron 35.0%
!!! GGGAAAGGTTTTGTTGTTTA+TGG - chr2_3:57565038-57565057 Msa0301470:intron 35.0%
TCAGAAAAATCAGGTTCTGT+TGG + chr2_3:57565146-57565165 None:intergenic 35.0%
GTCACACTCCCCTTAAAATA+TGG + chr2_3:57561027-57561046 None:intergenic 40.0%
ATGAACTAGAAACCAGTTGG+AGG + chr2_3:57561096-57561115 None:intergenic 40.0%
CCTATGAACTAGAAACCAGT+TGG + chr2_3:57561099-57561118 None:intergenic 40.0%
!! CCAACTGGTTTCTAGTTCAT+AGG - chr2_3:57561096-57561115 Msa0301470:three_prime_UTR 40.0%
!! TGGTTTCTAGTTCATAGGTG+TGG - chr2_3:57561101-57561120 Msa0301470:three_prime_UTR 40.0%
!! TTTCTAGTTCATAGGTGTGG+AGG - chr2_3:57561104-57561123 Msa0301470:three_prime_UTR 40.0%
GTGGCCACAAATATCAATGA+TGG + chr2_3:57561382-57561401 None:intergenic 40.0%
! GATTCTTTGGGAAACCAGTA+TGG - chr2_3:57561464-57561483 Msa0301470:CDS 40.0%
! TTCTTTGGGAAACCAGTATG+GGG - chr2_3:57561466-57561485 Msa0301470:CDS 40.0%
TGGATTATCAAACCCCATAC+TGG + chr2_3:57561481-57561500 None:intergenic 40.0%
GAAACACACCTTTCAGATCT+TGG + chr2_3:57561501-57561520 None:intergenic 40.0%
!! ACCTTAACTCCACTTGTTGT+GGG + chr2_3:57561658-57561677 None:intergenic 40.0%
CAAACTTCTTCTCTAACCAC+TGG + chr2_3:57561925-57561944 None:intergenic 40.0%
TTATGTTGCTCCTTCTTCCA+AGG - chr2_3:57562044-57562063 Msa0301470:intron 40.0%
CTACCAAAAACCTTGGAAGA+AGG + chr2_3:57562057-57562076 None:intergenic 40.0%
!!! TCTTCCAAGGTTTTTGGTAG+AGG - chr2_3:57562057-57562076 Msa0301470:intron 40.0%
TTCTCCTCTACCAAAAACCT+TGG + chr2_3:57562064-57562083 None:intergenic 40.0%
CCTAAGAGCGATCTTAGAAT+AGG + chr2_3:57562097-57562116 None:intergenic 40.0%
!! CCTATTCTAAGATCGCTCTT+AGG - chr2_3:57562094-57562113 Msa0301470:intron 40.0%
ACGAACAAATGATACGCTCA+AGG - chr2_3:57562380-57562399 Msa0301470:CDS 40.0%
CGAACAAATGATACGCTCAA+GGG - chr2_3:57562381-57562400 Msa0301470:CDS 40.0%
AGTGACACCGTCATACATTT+AGG - chr2_3:57562437-57562456 Msa0301470:intron 40.0%
CAACCTCATGCTTTACATGT+TGG - chr2_3:57562703-57562722 Msa0301470:intron 40.0%
TAGGCCAAATTTGGATAAGC+AGG + chr2_3:57562895-57562914 None:intergenic 40.0%
! CTGGAAAAGTGTGTGCAATA+TGG - chr2_3:57563103-57563122 Msa0301470:CDS 40.0%
GCTATAAACCAAGCTACTCT+CGG + chr2_3:57563182-57563201 None:intergenic 40.0%
TGATGTAACTTCGGAAGTCA+TGG + chr2_3:57563270-57563289 None:intergenic 40.0%
GAATGGCGATGATGTAACTT+CGG + chr2_3:57563279-57563298 None:intergenic 40.0%
AGTACTTCTATTGTCTCTGG+TGG - chr2_3:57563306-57563325 Msa0301470:intron 40.0%
GTACTTCTATTGTCTCTGGT+GGG - chr2_3:57563307-57563326 Msa0301470:intron 40.0%
TACTTCTATTGTCTCTGGTG+GGG - chr2_3:57563308-57563327 Msa0301470:intron 40.0%
! GTACTTTTCGCATTGCTGAT+TGG - chr2_3:57563586-57563605 Msa0301470:intron 40.0%
CGAAGGATGCATATGTTTCA+CGG + chr2_3:57563781-57563800 None:intergenic 40.0%
GAGGAAGAAGATCTTCTCAA+AGG - chr2_3:57564071-57564090 Msa0301470:intron 40.0%
ATTTCTCTCCTAAGATCCAG+CGG + chr2_3:57564101-57564120 None:intergenic 40.0%
GAACTAGTTCAAGACAAAGG+TGG + chr2_3:57564130-57564149 None:intergenic 40.0%
CTCGAACTAGTTCAAGACAA+AGG + chr2_3:57564133-57564152 None:intergenic 40.0%
CTTTGTCTTGAACTAGTTCG+AGG - chr2_3:57564131-57564150 Msa0301470:intron 40.0%
AAAGATCTGCGACTATGATC+TGG + chr2_3:57564312-57564331 None:intergenic 40.0%
!! ATGAAGAGCATTTCGTTCAC+TGG + chr2_3:57564600-57564619 None:intergenic 40.0%
! ATGAGTTGAAGAATCCGTCA+CGG + chr2_3:57564662-57564681 None:intergenic 40.0%
TGGTGAAGAGCTCTTAACAT+GGG - chr2_3:57564707-57564726 Msa0301470:intron 40.0%
TTATTCAAGCTGCATGGAGA+AGG - chr2_3:57564925-57564944 Msa0301470:intron 40.0%
GAAAGAAGCTGCTGAATTGA+GGG - chr2_3:57564959-57564978 Msa0301470:intron 40.0%
TTTATGGAACAAGGGTTGCA+AGG - chr2_3:57565054-57565073 Msa0301470:intron 40.0%
GTTGCAAGGAATACTAGGAA+AGG - chr2_3:57565068-57565087 Msa0301470:intron 40.0%
!! ATGCATTCTGGGACAAATTC+TGG - chr2_3:57565098-57565117 Msa0301470:intron 40.0%
!! TGCATTCTGGGACAAATTCT+GGG - chr2_3:57565099-57565118 Msa0301470:intron 40.0%
TTACTATGCCATGCACTTTG+TGG - chr2_3:57565292-57565311 Msa0301470:five_prime_UTR 40.0%
TATACTGCAACTCCTCCAAC+TGG - chr2_3:57561081-57561100 Msa0301470:three_prime_UTR 45.0%
GATAAGTTGCTTGCAGCAGA+GGG - chr2_3:57561126-57561145 Msa0301470:three_prime_UTR 45.0%
! TCAATGATGGTAACGCGCAA+AGG + chr2_3:57561369-57561388 None:intergenic 45.0%
TCCCACAACAAGTGGAGTTA+AGG - chr2_3:57561654-57561673 Msa0301470:CDS 45.0%
! CACCTTAACTCCACTTGTTG+TGG + chr2_3:57561659-57561678 None:intergenic 45.0%
! GCTCCTTCTTCCAAGGTTTT+TGG - chr2_3:57562051-57562070 Msa0301470:intron 45.0%
GCTCTTAGGTACTTCAGCAA+GGG - chr2_3:57562108-57562127 Msa0301470:intron 45.0%
! GGTACTTCAGCAAGGGTTTT+TGG - chr2_3:57562115-57562134 Msa0301470:intron 45.0%
AACAGACTCATAGGTACCTG+AGG + chr2_3:57562168-57562187 None:intergenic 45.0%
GACGACATCTGACACATTCA+AGG + chr2_3:57562218-57562237 None:intergenic 45.0%
ATGGTTGAACCCCTAAGTGT+TGG + chr2_3:57562545-57562564 None:intergenic 45.0%
AACACTTAGGGGTTCAACCA+TGG - chr2_3:57562544-57562563 Msa0301470:intron 45.0%
GGTTGTGACAGAAACAGCAT+GGG - chr2_3:57562667-57562686 Msa0301470:intron 45.0%
TGGCCAACATGTAAAGCATG+AGG + chr2_3:57562709-57562728 None:intergenic 45.0%
! TAGAACGTCAAGAAGCATGC+TGG - chr2_3:57563084-57563103 Msa0301470:CDS 45.0%
AACGGATAATAGCCGGCATT+TGG + chr2_3:57563230-57563249 None:intergenic 45.0%
! AATGCCGGCTATTATCCGTT+TGG - chr2_3:57563230-57563249 Msa0301470:CDS 45.0%
ATTGTCTCTGGTGGGGATTA+AGG - chr2_3:57563315-57563334 Msa0301470:intron 45.0%
GGAACCTGAGGCAAGTATTA+TGG - chr2_3:57563336-57563355 Msa0301470:intron 45.0%
! TTTGGTGGGTGCAGTTCTTT+GGG - chr2_3:57563493-57563512 Msa0301470:intron 45.0%
GCTATTGTGGTTGCAACTCT+TGG - chr2_3:57563559-57563578 Msa0301470:intron 45.0%
TGTGGTTGCAACTCTTGGAT+TGG - chr2_3:57563564-57563583 Msa0301470:intron 45.0%
TGGTAATATGCAGGTAAGCG+TGG - chr2_3:57563606-57563625 Msa0301470:intron 45.0%
! GACGAACGGTTATCGATTGA+AGG + chr2_3:57563926-57563945 None:intergenic 45.0%
TAACCGTTCGTCTAGAAGAG+TGG - chr2_3:57563934-57563953 Msa0301470:intron 45.0%
!! ATAGACTCTCGTAGTTCTGG+AGG + chr2_3:57564008-57564027 None:intergenic 45.0%
CGAATAGACTCTCGTAGTTC+TGG + chr2_3:57564011-57564030 None:intergenic 45.0%
TATAAATGGGTGGCTACACG+AGG - chr2_3:57564044-57564063 Msa0301470:intron 45.0%
TGATCTGGATCGCAACATCA+AGG + chr2_3:57564297-57564316 None:intergenic 45.0%
! GGTTCCCTTGTTTGACCAAA+TGG - chr2_3:57564491-57564510 Msa0301470:CDS 45.0%
AAAGCCTGCATTATGCACTG+AGG - chr2_3:57564548-57564567 Msa0301470:CDS 45.0%
AAGCCTGCATTATGCACTGA+GGG - chr2_3:57564549-57564568 Msa0301470:CDS 45.0%
GGAACTTATCTTGTTCGCGA+AGG - chr2_3:57564570-57564589 Msa0301470:CDS 45.0%
AACTCATGTAGAATCGGTCC+AGG - chr2_3:57564675-57564694 Msa0301470:CDS 45.0%
GTGGTGAAGAGCTCTTAACA+TGG - chr2_3:57564706-57564725 Msa0301470:intron 45.0%
!! CACACTTGGTCTTGGATCTA+AGG + chr2_3:57564733-57564752 None:intergenic 45.0%
! CATGGAGAAGGCACAAGAAA+AGG - chr2_3:57564937-57564956 Msa0301470:intron 45.0%
GGAAAGAAGCTGCTGAATTG+AGG - chr2_3:57564958-57564977 Msa0301470:intron 45.0%
CAAGGGTTGCAAGGAATACT+AGG - chr2_3:57565063-57565082 Msa0301470:intron 45.0%
!! GCATTCTGGGACAAATTCTG+GGG - chr2_3:57565100-57565119 Msa0301470:intron 45.0%
GACATCATCCACAAAGTGCA+TGG + chr2_3:57565303-57565322 None:intergenic 45.0%
!! TAAATATAATCAATATAAAT+GGG - chr2_3:57564031-57564050 Msa0301470:intron 5.0%
GGATAAGTTGCTTGCAGCAG+AGG - chr2_3:57561125-57561144 Msa0301470:three_prime_UTR 50.0%
TGCAGCAGAGGGAAATGCTT+TGG - chr2_3:57561137-57561156 Msa0301470:three_prime_UTR 50.0%
! GTGCTCCTTCCCTTTTGTGT+TGG - chr2_3:57561180-57561199 Msa0301470:CDS 50.0%
CGATGTATGAGTTGTCCCCT+AGG + chr2_3:57561835-57561854 None:intergenic 50.0%
GGGGACAACTCATACATCGT+TGG - chr2_3:57561836-57561855 Msa0301470:CDS 50.0%
CGCTCTTAGGTACTTCAGCA+AGG - chr2_3:57562107-57562126 Msa0301470:intron 50.0%
CTCATAGGTACCTGAGGAAG+AGG + chr2_3:57562162-57562181 None:intergenic 50.0%
GGGTTGTGACAGAAACAGCA+TGG - chr2_3:57562666-57562685 Msa0301470:intron 50.0%
AAGATGCTCCGAGAGTAGCT+TGG - chr2_3:57563171-57563190 Msa0301470:CDS 50.0%
ACTGCACCCACCAAACAGAA+AGG + chr2_3:57563488-57563507 None:intergenic 50.0%
! GTTTGGTGGGTGCAGTTCTT+TGG - chr2_3:57563492-57563511 Msa0301470:intron 50.0%
CCTCCACTCTTCTAGACGAA+CGG + chr2_3:57563940-57563959 None:intergenic 50.0%
CGTTCGTCTAGAAGAGTGGA+GGG - chr2_3:57563938-57563957 Msa0301470:intron 50.0%
TCTTCTCAAAGGACTTCCGC+TGG - chr2_3:57564082-57564101 Msa0301470:intron 50.0%
AAGGACTTCCGCTGGATCTT+AGG - chr2_3:57564090-57564109 Msa0301470:intron 50.0%
TGTCTCCACAACCGTGATTG+TGG + chr2_3:57564267-57564286 None:intergenic 50.0%
GTTCCCTCAGTGCATAATGC+AGG + chr2_3:57564555-57564574 None:intergenic 50.0%
ACAACAAATGGAGGCCGTGA+CGG - chr2_3:57564645-57564664 Msa0301470:CDS 50.0%
TCTTCACCACAGAAATCGCC+TGG + chr2_3:57564696-57564715 None:intergenic 50.0%
!! GGGAGTATCACACTTGGTCT+TGG + chr2_3:57564741-57564760 None:intergenic 50.0%
!! GAGGAAGGGAGTATCACACT+TGG + chr2_3:57564747-57564766 None:intergenic 50.0%
AGCTGCTGAATTGAGGGCTA+AGG - chr2_3:57564965-57564984 Msa0301470:intron 50.0%
GAGGGCTAAGGAGAATCTTG+TGG - chr2_3:57564977-57564996 Msa0301470:intron 50.0%
GAATCTTGTGGCAGCTTCAG+AGG - chr2_3:57564989-57565008 Msa0301470:intron 50.0%
! TGGTAACGCGCAAAGGCGAT+TGG + chr2_3:57561362-57561381 None:intergenic 55.0%
TGCTGCATTGCCTCTTCCTC+AGG - chr2_3:57562149-57562168 Msa0301470:intron 55.0%
GTGACAGAAACAGCATGGGC+AGG - chr2_3:57562671-57562690 Msa0301470:intron 55.0%
GGTGGGGATTAAGGAACCTG+AGG - chr2_3:57563324-57563343 Msa0301470:intron 55.0%
!! GGGTGCAGTTCTTTGGGACA+AGG - chr2_3:57563499-57563518 Msa0301470:intron 55.0%
CCGTTCGTCTAGAAGAGTGG+AGG - chr2_3:57563937-57563956 Msa0301470:intron 55.0%
GGCTGAAGCAGCCACAATCA+CGG - chr2_3:57564253-57564272 Msa0301470:CDS 55.0%
AGCAGCCACAATCACGGTTG+TGG - chr2_3:57564259-57564278 Msa0301470:CDS 55.0%
ATCGGTCCAGGCGATTTCTG+TGG - chr2_3:57564687-57564706 Msa0301470:CDS 55.0%
GGTGGCTACACGAGGAGTAG+AGG - chr2_3:57564052-57564071 Msa0301470:intron 60.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 57561023 57565324 57561023 ID=Msa0301470;Name=Msa0301470
chr2_3 mRNA 57561023 57565324 57561023 ID=Msa0301470-mRNA-1;Parent=Msa0301470;Name=Msa0301470-mRNA-1;_AED=0.05;_eAED=0.05;_QI=236|0.85|0.75|1|0.85|0.75|8|152|690
chr2_3 exon 57561023 57561855 57561023 ID=Msa0301470-mRNA-1:exon:18555;Parent=Msa0301470-mRNA-1
chr2_3 exon 57562193 57562429 57562193 ID=Msa0301470-mRNA-1:exon:18554;Parent=Msa0301470-mRNA-1
chr2_3 exon 57562729 57562840 57562729 ID=Msa0301470-mRNA-1:exon:18553;Parent=Msa0301470-mRNA-1
chr2_3 exon 57563041 57563294 57563041 ID=Msa0301470-mRNA-1:exon:18552;Parent=Msa0301470-mRNA-1
chr2_3 exon 57563614 57563826 57563614 ID=Msa0301470-mRNA-1:exon:18551;Parent=Msa0301470-mRNA-1
chr2_3 exon 57564177 57564723 57564177 ID=Msa0301470-mRNA-1:exon:18550;Parent=Msa0301470-mRNA-1
chr2_3 exon 57564836 57564911 57564836 ID=Msa0301470-mRNA-1:exon:18549;Parent=Msa0301470-mRNA-1
chr2_3 exon 57565136 57565324 57565136 ID=Msa0301470-mRNA-1:exon:18548;Parent=Msa0301470-mRNA-1
chr2_3 five_prime_UTR 57565136 57565324 57565136 ID=Msa0301470-mRNA-1:five_prime_utr;Parent=Msa0301470-mRNA-1
chr2_3 five_prime_UTR 57564865 57564911 57564865 ID=Msa0301470-mRNA-1:five_prime_utr;Parent=Msa0301470-mRNA-1
chr2_3 CDS 57564836 57564864 57564836 ID=Msa0301470-mRNA-1:cds;Parent=Msa0301470-mRNA-1
chr2_3 CDS 57564177 57564723 57564177 ID=Msa0301470-mRNA-1:cds;Parent=Msa0301470-mRNA-1
chr2_3 CDS 57563614 57563826 57563614 ID=Msa0301470-mRNA-1:cds;Parent=Msa0301470-mRNA-1
chr2_3 CDS 57563041 57563294 57563041 ID=Msa0301470-mRNA-1:cds;Parent=Msa0301470-mRNA-1
chr2_3 CDS 57562729 57562840 57562729 ID=Msa0301470-mRNA-1:cds;Parent=Msa0301470-mRNA-1
chr2_3 CDS 57562193 57562429 57562193 ID=Msa0301470-mRNA-1:cds;Parent=Msa0301470-mRNA-1
chr2_3 CDS 57561175 57561855 57561175 ID=Msa0301470-mRNA-1:cds;Parent=Msa0301470-mRNA-1
chr2_3 three_prime_UTR 57561023 57561174 57561023 ID=Msa0301470-mRNA-1:three_prime_utr;Parent=Msa0301470-mRNA-1
Gene Sequence

>Msa0301470

ATGGGGTTTGATAATCCAAGATCTGAAAGGTTTGAAGATGATCCTGAAATATCAAAAATTCCCACAACAAGTGGAGTTAAGGTGAAATACCACATTGATGGAACACAAATACCTGAACAAAGTTCCAAAAAGTCTCAAAAGAATGCTACAAGGAACAAGTTTTTGAAAACAAGAGTATTGTCAAGAGTATTCTCAGAAGACTATGAAAGAGTAAAAAAAAGAGTGTTAGTATTAGATCCTAGGGGACAACTCATACATCGTTGGAACAAAATATTTTTAGTAGCATGTTTAGTTTCTTTGTTTGTGGATCCACTGTTCTTTTATTTGCCAGTGGTTAGAGAAGAAGTTTGCATTGATATTGGAAAAACTCTTGAAGTTATTCTCACAGTTGTTAGATCATTTGGAGATTTGTTTTACATGATTCAGATTTGTATGAAGTTTCGTACTGCTTATGTTGCTCCTTCTTCCAAGGTTTTTGGTAGAGGAGAACTTGTTCTTACCTATTCTAAGATCGCTCTTAGGTACTTCAGCAAGGGTTTTTGGTTAGACTCTATTGCTGCATTGCCTCTTCCTCAGGTGTTGATTTGGATTATTATACCAACACTTAGGGGTTCAACCATGGCAAATACGAAAAATGTCCTTCGTTTTTTCATCATTTTTCAATATATTCCGAGGCTGTATTTGATTTTTCCACTTTCATCACAAATTGTAAAAGCTACAGGGGTTGTGACAGAAACAGCATGGGCAGGTGCTGCTTACAACCTCATGCTTTACATGTTGGCCAGCCATATTTTAGGAGCTTGCTGGTACCTTCTATCAATAGAACGTCAAGAAGCATGCTGGAAAAGTGTGTGCAATATGGAGAAATCAAATTGTCAATATGGATTCTTCAATTGTCACAGTGTTAAAGATGCTCCGAGAGTAGCTTGGTTTATAGCTAGTAATGTCACAAATTTATGTTCACCAAATGCCGGCTATTATCCGTTTGGTATATATGCTGATGCCATGACTTCCGAAGTTACATCATCGCCATTCTTCAATAATTCTTTGGGACAAGGTCTTCTTACTAGCACTTTTATCGGAGAAATAATGGTTGCTATTGTGGTTGCAACTCTTGGATTGGTACTTTTCGCATTGCTGATTGGTAATATGCAGACATACCTTCAATCGATAACCGTTCGTCTAGAAGAGTGGAGGGTGAAAAGAACTGACACAGAACAATGGATGCATCACAGACAACTACCTCCAGAACTACGAGAGTCTATTCGTAAATATAATCAATATAAATGGGTGGCTACACGAGGAGTAGAGGAAGAAGATCTTCTCAAAGGACTTCCGCTGGATCTTAGGAGAGAAATCAAACGCCACCTTTGTCTTGAACTAGTTCGAGGAGTTCCCTTGTTTGACCAAATGGATGAAAGAATGCTAGACGCAATATGCGAAAGACTAAAGCCTGCATTATGCACTGAGGGAACTTATCTTGTTCGCGAAGGCGATCCAGTGAACGAAATGCTCTTCATAATTAGAGGCCATCTTGATTCATACACAACAAATGGAGGCCGTGACGGATTCTTCAACTCATGTAGAATCGGTCCAGGCGATTTCTGTGGTGAAGAGCTCTTAACATGGGCCTTAGATCCAAGACCAAGTGTGATACTCCCTTCCTCAACAAGAACAGTTAAAGCTTTCTCAGAAGTAGAAGCCTTTGCACTTATAGCTGAAGATCTTAAGTTTGTTGCATCTCAATTTAGAAGACTACATAGTAAACAACTTAGGCATAAATTCAGGTTTTATTCACATCAATGGAGAACTTGGGCTGCTTGTTTTATTCAAGCTGCATGGAGAAGGCACAAGAAAAGGAAAGAAGCTGCTGAATTGAGGGCTAAGGAGAATCTTGTGGCAGCTTCAGAGGCTGAAAATGAAATAGCAAAAAAATATGGGAAAGGTTTTGTTGTTTATGGAACAAGGGTTGCAAGGAATACTAGGAAAGGTGTTAATATGCATTCTGGGACAAATTCTGGGGTAGTAAGCTCTTTGCAGAAACCAACAGAACCTGATTTTTCTGATGAATAA
Protein sequence

>Msa0301470

MGFDNPRSERFEDDPEISKIPTTSGVKVKYHIDGTQIPEQSSKKSQKNATRNKFLKTRVLSRVFSEDYERVKKRVLVLDPRGQLIHRWNKIFLVACLVSLFVDPLFFYLPVVREEVCIDIGKTLEVILTVVRSFGDLFYMIQICMKFRTAYVAPSSKVFGRGELVLTYSKIALRYFSKGFWLDSIAALPLPQVLIWIIIPTLRGSTMANTKNVLRFFIIFQYIPRLYLIFPLSSQIVKATGVVTETAWAGAAYNLMLYMLASHILGACWYLLSIERQEACWKSVCNMEKSNCQYGFFNCHSVKDAPRVAWFIASNVTNLCSPNAGYYPFGIYADAMTSEVTSSPFFNNSLGQGLLTSTFIGEIMVAIVVATLGLVLFALLIGNMQTYLQSITVRLEEWRVKRTDTEQWMHHRQLPPELRESIRKYNQYKWVATRGVEEEDLLKGLPLDLRREIKRHLCLELVRGVPLFDQMDERMLDAICERLKPALCTEGTYLVREGDPVNEMLFIIRGHLDSYTTNGGRDGFFNSCRIGPGDFCGEELLTWALDPRPSVILPSSTRTVKAFSEVEAFALIAEDLKFVASQFRRLHSKQLRHKFRFYSHQWRTWAACFIQAAWRRHKKRKEAAELRAKENLVAASEAENEIAKKYGKGFVVYGTRVARNTRKGVNMHSGTNSGVVSSLQKPTEPDFSDE*