AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1406210

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1406210 MtrunA17_Chr2g0326871 95.907 562 6 2 70 614 142 703 0.0 1113
Msa1406210 MtrunA17_Chr2g0326871 56.604 159 56 4 1 158 1 147 5.12e-38 149
Msa1406210 MtrunA17_Chr4g0028861 81.414 495 79 1 71 552 190 684 0.0 849
Msa1406210 MtrunA17_Chr1g0181121 61.189 572 194 4 71 614 110 681 0.0 742
Msa1406210 MtrunA17_Chr2g0305771 59.408 574 203 5 71 614 114 687 0.0 684
Msa1406210 MtrunA17_Chr3g0139071 58.204 579 207 5 71 614 144 722 0.0 671
Msa1406210 MtrunA17_Chr7g0228181 54.641 571 232 5 71 614 110 680 0.0 644
Msa1406210 MtrunA17_Chr3g0125651 52.048 586 237 6 71 612 102 687 0.0 611
Msa1406210 MtrunA17_Chr1g0158861 53.390 590 234 8 57 614 134 714 0.0 605
Msa1406210 MtrunA17_Chr3g0126541 52.922 599 245 9 46 614 120 711 0.0 605
Msa1406210 MtrunA17_Chr4g0073941 53.968 567 234 7 71 614 166 728 0.0 602
Msa1406210 MtrunA17_Chr4g0001481 53.310 574 234 9 70 612 167 737 0.0 580
Msa1406210 MtrunA17_Chr7g0275581 52.930 546 228 8 70 593 149 687 0.0 545
Msa1406210 MtrunA17_Chr8g0347121 47.333 600 256 7 70 611 126 723 3.98e-177 520
Msa1406210 MtrunA17_Chr2g0326861 94.203 207 12 0 79 285 14 220 8.31e-141 408
Msa1406210 MtrunA17_Chr8g0360821 37.087 515 271 16 71 548 129 627 2.15e-99 317
Msa1406210 MtrunA17_Chr2g0318561 36.885 488 265 10 120 574 2 479 4.14e-99 311
Msa1406210 MtrunA17_Chr6g0481021 37.911 517 282 10 68 553 187 695 1.53e-96 310
Msa1406210 MtrunA17_Chr6g0480851 37.132 509 286 11 71 553 251 751 2.72e-88 290
Msa1406210 MtrunA17_Chr7g0218341 34.857 525 287 14 73 559 179 686 3.64e-88 288
Msa1406210 MtrunA17_Chr6g0480781 35.560 509 294 12 71 553 236 736 1.15e-85 283
Msa1406210 MtrunA17_Chr6g0480671 37.859 523 275 13 71 559 260 766 8.37e-85 281
Msa1406210 MtrunA17_Chr2g0326851 90.076 131 9 1 488 614 3 133 1.63e-77 242
Msa1406210 MtrunA17_Chr6g0480741 33.075 517 302 16 68 553 179 682 3.82e-66 229
Msa1406210 MtrunA17_Chr6g0480681 30.345 580 321 18 71 591 181 736 1.84e-61 217
Msa1406210 MtrunA17_Chr5g0395421 33.244 373 203 13 180 526 3 355 1.19e-42 157
Msa1406210 MtrunA17_Chr6g0480771 35.573 253 138 8 309 547 12 253 8.89e-34 130
Msa1406210 MtrunA17_Chr5g0395411 44.444 135 73 2 70 203 225 358 7.47e-30 121
Msa1406210 MtrunA17_Chr6g0480841 40.404 198 98 7 363 547 2 192 9.14e-30 117
Msa1406210 MtrunA17_Chr6g0480821 27.211 147 101 3 121 264 25 168 1.94e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1406210 AT2G28260.1 72.581 558 128 3 70 614 133 678 0.0 837
Msa1406210 AT2G28260.1 41.584 101 42 4 1 101 1 84 6.03e-11 65.9
Msa1406210 AT2G28260.2 72.581 558 128 3 70 614 93 638 0.0 835
Msa1406210 AT4G30360.1 56.747 578 216 5 71 614 140 717 0.0 659
Msa1406210 AT2G24610.1 56.947 583 210 6 71 612 141 723 0.0 654
Msa1406210 AT2G24610.2 56.947 583 210 6 71 612 156 738 0.0 652
Msa1406210 AT4G30560.1 55.810 568 220 8 71 614 170 730 0.0 625
Msa1406210 AT4G30560.4 55.810 568 220 8 71 614 170 730 0.0 625
Msa1406210 AT4G30560.5 55.810 568 220 8 71 614 144 704 0.0 625
Msa1406210 AT4G30560.2 55.810 568 220 8 71 614 144 704 0.0 625
Msa1406210 AT4G30560.3 55.810 568 220 8 71 614 144 704 0.0 625
Msa1406210 AT2G23980.9 54.671 578 226 6 71 614 170 745 0.0 624
Msa1406210 AT2G23980.10 54.671 578 226 6 71 614 170 745 0.0 624
Msa1406210 AT2G23980.1 54.671 578 226 6 71 614 170 745 0.0 624
Msa1406210 AT2G23980.4 54.671 578 226 6 71 614 170 745 0.0 624
Msa1406210 AT2G23980.5 54.671 578 226 6 71 614 170 745 0.0 624
Msa1406210 AT2G23980.6 54.671 578 226 6 71 614 143 718 0.0 624
Msa1406210 AT5G57940.3 54.225 568 231 5 71 614 148 710 0.0 613
Msa1406210 AT5G14870.1 50.924 595 239 7 71 612 106 700 0.0 613
Msa1406210 AT5G57940.1 54.225 568 231 5 71 614 155 717 0.0 613
Msa1406210 AT5G57940.2 54.225 568 231 5 71 614 155 717 0.0 613
Msa1406210 AT2G23980.3 52.579 601 226 7 71 614 170 768 0.0 610
Msa1406210 AT2G23980.2 52.579 601 226 7 71 614 170 768 0.0 610
Msa1406210 AT2G23980.8 52.579 601 226 7 71 614 170 768 0.0 610
Msa1406210 AT2G23980.7 52.579 601 226 7 71 614 170 768 0.0 610
Msa1406210 AT1G15990.1 53.663 587 225 8 70 614 157 738 0.0 605
Msa1406210 AT5G53130.1 54.466 571 227 5 71 614 151 715 0.0 602
Msa1406210 AT1G19780.1 53.516 583 229 8 70 611 163 744 0.0 602
Msa1406210 AT3G48010.1 55.731 506 202 4 71 554 111 616 0.0 586
Msa1406210 AT1G01340.2 50.947 581 235 9 71 612 135 704 0.0 583
Msa1406210 AT1G01340.1 50.947 581 235 9 71 612 130 699 0.0 582
Msa1406210 AT4G01010.1 50.083 601 267 9 37 612 100 692 0.0 577
Msa1406210 AT4G01010.2 50.083 601 267 9 37 612 100 692 0.0 577
Msa1406210 AT2G46430.1 50.088 569 250 9 71 611 139 701 0.0 548
Msa1406210 AT2G46430.2 50.088 569 250 9 71 611 139 701 0.0 548
Msa1406210 AT1G19780.2 52.908 533 209 8 120 611 2 533 0.0 538
Msa1406210 AT2G46440.1 44.886 528 261 11 77 582 102 621 3.81e-133 403
Msa1406210 AT2G46440.2 46.756 447 213 8 157 582 1 443 4.92e-120 363
Msa1406210 AT2G46450.3 39.564 551 289 14 67 591 52 584 1.14e-119 368
Msa1406210 AT2G46450.2 39.564 551 289 14 67 591 65 597 1.31e-119 369
Msa1406210 AT2G46450.4 39.851 537 283 13 77 591 102 620 2.90e-118 366
Msa1406210 AT2G46450.1 39.851 537 283 13 77 591 102 620 2.90e-118 366
Msa1406210 AT5G54250.2 37.347 490 263 13 97 554 177 654 1.07e-95 308
Msa1406210 AT5G54250.1 37.347 490 263 13 97 554 177 654 1.07e-95 308
Msa1406210 AT5G54250.4 37.347 490 263 13 97 554 177 654 1.07e-95 308
Msa1406210 AT3G17690.1 38.933 506 267 16 71 548 225 716 2.42e-95 308
Msa1406210 AT3G17700.1 40.080 499 260 14 71 542 259 745 4.67e-91 298
Msa1406210 AT5G54250.3 36.777 484 257 14 97 548 177 643 2.31e-90 294
Msa1406210 AT5G15410.2 36.111 504 278 12 73 542 183 676 1.80e-86 285
Msa1406210 AT5G15410.1 35.968 506 280 12 71 542 182 677 2.18e-86 285
Msa1406210 AT3G17690.2 39.336 422 223 12 71 473 225 632 6.33e-83 273
Msa1406210 AT3G17700.2 40.566 424 225 11 71 479 259 670 2.22e-80 268
Msa1406210 AT3G17690.3 38.462 299 158 9 71 350 225 516 2.99e-50 182

Find 135 sgRNAs with CRISPR-Local

Find 263 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAGAGAAATTTGATTCTT+TGG 0.173457 tig0023205:+27578 Msa1406210:five_prime_UTR
GGAATATTTCTCACTTTAAT+TGG 0.178020 tig0023205:+27287 Msa1406210:five_prime_UTR
GTAAATATAATCAATATAAA+TGG 0.208829 tig0023205:+30290 Msa1406210:CDS
GTTTGGAGGGTGCAGTTCTT+TGG 0.210884 tig0023205:+29747 Msa1406210:intron
AAAGGTGTTAATATGCATTC+TGG 0.262434 tig0023205:+31345 Msa1406210:CDS
GTCACACTCCCCTTAAAATA+TGG 0.279845 tig0023205:-27153 None:intergenic
GAGAAATCAAATTGTCAATA+TGG 0.294895 tig0023205:+29384 Msa1406210:CDS
CGAATAGACTCTCGTAGTTC+TGG 0.297309 tig0023205:-30268 None:intergenic
TATTATTGCAGGTGTTGATT+TGG 0.298468 tig0023205:+28637 Msa1406210:intron
TTTGGAGGGTGCAGTTCTTT+GGG 0.302499 tig0023205:+29748 Msa1406210:intron
AAGGACTTCCGCTGGATCTT+AGG 0.302671 tig0023205:+30350 Msa1406210:CDS
ATTCTTTGGGAAAACCAGTA+TGG 0.304135 tig0023205:+27592 Msa1406210:exon
ATTGTGCAATTTGTTTGTTT+TGG 0.304710 tig0023205:+31455 Msa1406210:three_prime_UTR
CACACTTGGTCTTGGATCTA+AGG 0.318439 tig0023205:-30989 None:intergenic
GAAGAGAAATTTGATTCTTT+GGG 0.321524 tig0023205:+27579 Msa1406210:five_prime_UTR
CCTATTCTAAGATTGCTCTT+AGG 0.322226 tig0023205:+28222 Msa1406210:CDS
CTAGCATTCTTTCATCCATT+TGG 0.323336 tig0023205:-30765 None:intergenic
GAAGCACACCTTTCAGATCT+TGG 0.324891 tig0023205:-27626 None:intergenic
CTTGACGTTCTATTGATAGA+AGG 0.346761 tig0023205:-29332 None:intergenic
GCTATTGTGGTTGCAACTCT+TGG 0.355052 tig0023205:+29814 Msa1406210:CDS
GAAATGCTCTTCATAATTAG+AGG 0.358622 tig0023205:+30865 Msa1406210:CDS
TGCTGCATTGCCTCTTCCTC+AGG 0.378993 tig0023205:+28277 Msa1406210:CDS
TGCAGCAGAGGGAAATGCTT+TGG 0.385038 tig0023205:+27266 Msa1406210:five_prime_UTR
TTCATATATAAATTAGAGAT+AGG 0.385858 tig0023205:+31486 Msa1406210:three_prime_UTR
TGGATTATCAAATCCCATAC+TGG 0.390216 tig0023205:-27606 None:intergenic
CAAGGGTTGCAAGGAATACT+AGG 0.391426 tig0023205:+31322 Msa1406210:CDS
CCAACTGGTTTCTAGTTCAT+AGG 0.394399 tig0023205:+27225 Msa1406210:five_prime_UTR
GGTTCCCTTGTTTGACCAAA+TGG 0.396986 tig0023205:+30750 Msa1406210:intron
ATTCACACCAATGGAGAACT+TGG 0.401526 tig0023205:+31151 Msa1406210:CDS
ATGAAGAGCATTTCGTTCAC+TGG 0.405862 tig0023205:-30856 None:intergenic
GATTATTATACCAACACTTA+GGG 0.406775 tig0023205:+28659 Msa1406210:CDS
TCCCACAACAAGTGGAGTTA+AGG 0.407478 tig0023205:+27782 Msa1406210:CDS
GGATTATTATACCAACACTT+AGG 0.409310 tig0023205:+28658 Msa1406210:CDS
AACGGATAAAAGCCGGCATT+TGG 0.410069 tig0023205:-29486 None:intergenic
TCACAAATTGTAAAAGCTAC+AGG 0.415804 tig0023205:+28771 Msa1406210:CDS
CCTAAGAGCAATCTTAGAAT+AGG 0.417424 tig0023205:-28222 None:intergenic
CACCTTAACTCCACTTGTTG+TGG 0.420551 tig0023205:-27784 None:intergenic
CTTGATTCATACACAACAAA+TGG 0.422331 tig0023205:+30892 Msa1406210:CDS
GGGAGTATCACACTTGGTCT+TGG 0.424283 tig0023205:-30997 None:intergenic
TTTACAATTTGTGATGAAAG+TGG 0.430401 tig0023205:-28762 None:intergenic
ACCTTAACTCCACTTGTTGT+GGG 0.432233 tig0023205:-27783 None:intergenic
TTGGAAAAGTGTGTGCAATA+TGG 0.436003 tig0023205:+29362 Msa1406210:CDS
TTAACTGTTCTTGTGGAAGA+AGG 0.448046 tig0023205:-31018 None:intergenic
CTTTGTCTTGAACTAGTTCG+AGG 0.455073 tig0023205:+30391 Msa1406210:CDS
CATGGAGAAGGCACAAGAAA+AGG 0.459490 tig0023205:+31196 Msa1406210:CDS
GGAAAGAAGCTGCTGAATTG+AGG 0.459874 tig0023205:+31217 Msa1406210:CDS
AAGGTGTTAATATGCATTCT+GGG 0.461316 tig0023205:+31346 Msa1406210:CDS
TGTGGTTGCAACTCTTGGAT+TGG 0.461815 tig0023205:+29819 Msa1406210:CDS
GAAAGAAGCTGCTGAATTGA+GGG 0.466076 tig0023205:+31218 Msa1406210:CDS
ACTATGCATGATGCACTTTG+TGG 0.470656 tig0023205:+31544 Msa1406210:three_prime_UTR
TAAATATAATCAATATAAAT+GGG 0.474688 tig0023205:+30291 Msa1406210:CDS
AAGGTGAAATACCACATTGA+TGG 0.478709 tig0023205:+27801 Msa1406210:CDS
CAACCTCATGCTTTACATGT+TGG 0.485217 tig0023205:+28830 Msa1406210:CDS
TTCTTCAACTCATGTAGAAT+CGG 0.487881 tig0023205:+30928 Msa1406210:CDS
AGAAATAATGGTTGCTATTG+TGG 0.489273 tig0023205:+29801 Msa1406210:CDS
TTCATCCATTTGGTCAAACA+AGG 0.490363 tig0023205:-30755 None:intergenic
CTCATAGGTACCTGAGGAAG+AGG 0.499692 tig0023205:-28287 None:intergenic
TAGAAGTACTTATTGAAGAA+TGG 0.503317 tig0023205:-29552 None:intergenic
TATACTGCAACTCCTCCAAC+TGG 0.503848 tig0023205:+27210 Msa1406210:five_prime_UTR
GACGAACGGTTATCGATTGA+AGG 0.504792 tig0023205:-30183 None:intergenic
TTATGTTGCTCCTTCTTCGA+AGG 0.509627 tig0023205:+28172 Msa1406210:CDS
TTCTTTGGGAAAACCAGTAT+GGG 0.512800 tig0023205:+27593 Msa1406210:exon
GAATCTTGTGGCAGCTTCAG+AGG 0.513529 tig0023205:+31248 Msa1406210:CDS
GCACTTTGTGGATGATGTCT+TGG 0.518977 tig0023205:+31556 Msa1406210:three_prime_UTR
TTGTGATATACTTACATGGC+TGG 0.519386 tig0023205:-28853 None:intergenic
AGCTGCTGAATTGAGGGCTA+AGG 0.522860 tig0023205:+31224 Msa1406210:CDS
CACAAATTGTAAAAGCTACA+GGG 0.525537 tig0023205:+28772 Msa1406210:CDS
TTATTCAAGCTGCATGGAGA+AGG 0.526489 tig0023205:+31184 Msa1406210:CDS
TAACTGTTCTTGTGGAAGAA+GGG 0.531786 tig0023205:-31017 None:intergenic
GGGTGCAGTTCTTTGGGACA+AGG 0.533291 tig0023205:+29754 Msa1406210:intron
AACTCATGTAGAATCGGTCC+AGG 0.535190 tig0023205:+30934 Msa1406210:CDS
AATTTATGTTCACCAAATGC+CGG 0.535721 tig0023205:+29474 Msa1406210:CDS
GAAAGCTTTAACTGTTCTTG+TGG 0.538709 tig0023205:-31025 None:intergenic
TGCTCTTAGGTACTTCAGCA+AGG 0.549675 tig0023205:+28235 Msa1406210:CDS
TGATGTAACTTCGGAAGTCA+TGG 0.553176 tig0023205:-29526 None:intergenic
TCTTCTCAAAGGACTTCCGC+TGG 0.553705 tig0023205:+30342 Msa1406210:CDS
TATACCAAACGGATAAAAGC+CGG 0.557769 tig0023205:-29493 None:intergenic
TTTATGGAACAAGGGTTGCA+AGG 0.560809 tig0023205:+31313 Msa1406210:CDS
TCTTCACCACAGAAATCGCC+TGG 0.562215 tig0023205:-30952 None:intergenic
ATTGCTGATTGGTAATATGC+AGG 0.564527 tig0023205:+29852 Msa1406210:CDS
TTCACACCAATGGAGAACTT+GGG 0.569483 tig0023205:+31152 Msa1406210:CDS
GGATAAGTTGCTTGCAGCAG+AGG 0.569822 tig0023205:+27254 Msa1406210:five_prime_UTR
GCATCAGCATATATACCAAA+CGG 0.570370 tig0023205:-29504 None:intergenic
AAGCAGCCCAAGTTCTCCAT+TGG 0.571876 tig0023205:-31158 None:intergenic
GTTCCCTCAGTGCATAATGC+AGG 0.571915 tig0023205:-30811 None:intergenic
ACAACAAATGGAGGCCGTGA+CGG 0.574379 tig0023205:+30904 Msa1406210:CDS
GAGGAAGAAGATCTTCTCAA+AGG 0.575171 tig0023205:+30331 Msa1406210:CDS
TAGAACGTCAAGAAGCATGT+TGG 0.581133 tig0023205:+29343 Msa1406210:CDS
GCTCTTAGGTACTTCAGCAA+GGG 0.584716 tig0023205:+28236 Msa1406210:CDS
TGGTTTCTAGTTCATAGGTG+TGG 0.586045 tig0023205:+27230 Msa1406210:five_prime_UTR
GAATGGCGATGATGTAACTT+CGG 0.588257 tig0023205:-29535 None:intergenic
GGGTTGTTACAGAAACAGCA+TGG 0.594816 tig0023205:+28793 Msa1406210:CDS
GTTACAGAAACAGCATGGGC+AGG 0.595665 tig0023205:+28798 Msa1406210:CDS
CCTATGAACTAGAAACCAGT+TGG 0.597683 tig0023205:-27225 None:intergenic
GCTATAAACCAAGCTACTCT+CGG 0.598310 tig0023205:-29438 None:intergenic
GGTGGCTACACGAGGAGTAG+AGG 0.598943 tig0023205:+30312 Msa1406210:CDS
TTTCTAGTTCATAGGTGTGG+AGG 0.600735 tig0023205:+27233 Msa1406210:five_prime_UTR
ATGAACTAGAAACCAGTTGG+AGG 0.603367 tig0023205:-27222 None:intergenic
GGAACTTATCTTGTTCGCGA+AGG 0.606182 tig0023205:+30829 Msa1406210:CDS
TTGTTGTGTATGAATCAAGA+TGG 0.607110 tig0023205:-30888 None:intergenic
GTGGTGAAGAGCTCTTAACA+TGG 0.607722 tig0023205:+30965 Msa1406210:CDS
TAACCGTTCGTCTAGAAGAG+TGG 0.612435 tig0023205:+30194 Msa1406210:CDS
AAGATGCTCCGAGAGTAGCT+TGG 0.612832 tig0023205:+29430 Msa1406210:CDS
CCTCCACTCTTCTAGACGAA+CGG 0.613285 tig0023205:-30197 None:intergenic
TCATCCATTTGGTCAAACAA+GGG 0.614441 tig0023205:-30754 None:intergenic
TTGATAATCCAAGATCTGAA+AGG 0.616294 tig0023205:+27618 Msa1406210:CDS
GACTACATAGTAAACAACTT+AGG 0.617133 tig0023205:+31112 Msa1406210:CDS
AAGCCTGCATTATGCACTGA+GGG 0.618536 tig0023205:+30808 Msa1406210:CDS
GATAAGTTGCTTGCAGCAGA+GGG 0.620001 tig0023205:+27255 Msa1406210:five_prime_UTR
AGTCTCAAAAGAATGCTACA+AGG 0.630706 tig0023205:+27853 Msa1406210:CDS
CGTTCGTCTAGAAGAGTGGA+GGG 0.630762 tig0023205:+30198 Msa1406210:CDS
AACACTTAGGGGTTCAACCA+TGG 0.634663 tig0023205:+28671 Msa1406210:CDS
CCGTTCGTCTAGAAGAGTGG+AGG 0.639971 tig0023205:+30197 Msa1406210:CDS
AAAGAACTGACACAGAACAA+TGG 0.642598 tig0023205:+30224 Msa1406210:CDS
ATAGACTCTCGTAGTTCTGG+AGG 0.643923 tig0023205:-30265 None:intergenic
ATCGGTCCAGGCGATTTCTG+TGG 0.645701 tig0023205:+30946 Msa1406210:CDS
GATTCATACACAACAAATGG+AGG 0.646565 tig0023205:+30895 Msa1406210:CDS
ATATAATCAATATAAATGGG+TGG 0.651864 tig0023205:+30294 Msa1406210:CDS
GAGGGCTAAGGAGAATCTTG+TGG 0.656336 tig0023205:+31236 Msa1406210:CDS
ATTATTATACCAACACTTAG+GGG 0.657353 tig0023205:+28660 Msa1406210:CDS
TGGTGAAGAGCTCTTAACAT+GGG 0.661791 tig0023205:+30966 Msa1406210:CDS
CTCGAACTAGTTCAAGACAA+AGG 0.662676 tig0023205:-30390 None:intergenic
GGTTGTTACAGAAACAGCAT+GGG 0.662855 tig0023205:+28794 Msa1406210:CDS
ACAAATTGTAAAAGCTACAG+GGG 0.665070 tig0023205:+28773 Msa1406210:CDS
ATTTCTCTCCTAAGATCCAG+CGG 0.666530 tig0023205:-30358 None:intergenic
GTTGCAAGGAATACTAGGAA+AGG 0.669215 tig0023205:+31327 Msa1406210:CDS
ATGGTTGAACCCCTAAGTGT+TGG 0.671733 tig0023205:-28669 None:intergenic
GAAGAAGGGAGTATCACACT+TGG 0.672182 tig0023205:-31003 None:intergenic
AGGTGTTAATATGCATTCTG+GGG 0.675385 tig0023205:+31347 Msa1406210:CDS
TATAAATGGGTGGCTACACG+AGG 0.677991 tig0023205:+30304 Msa1406210:CDS
ATGAGTTGAAGAATCCGTCA+CGG 0.683468 tig0023205:-30918 None:intergenic
GTATTTGTGTTCCATCAATG+TGG 0.691831 tig0023205:-27812 None:intergenic
GAACTAGTTCAAGACAAAGG+TGG 0.711572 tig0023205:-30387 None:intergenic
TGGCCAACATGTAAAGCATG+AGG 0.724789 tig0023205:-28833 None:intergenic
AAAGCCTGCATTATGCACTG+AGG 0.736004 tig0023205:+30807 Msa1406210:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATATTTTTATAAGATATTA+TGG - tig0023205:29070-29089 None:intergenic 10.0%
!! GTAAATATAATCAATATAAA+TGG + tig0023205:30290-30309 Msa1406210:CDS 10.0%
!! ATATATATTCCAACAAAAAA+GGG - tig0023205:27321-27340 None:intergenic 15.0%
!!! CTTTTCATTTTATTTTTCAT+AGG + tig0023205:27730-27749 Msa1406210:intron 15.0%
!! TCTGTTTATTTATGTTTAAA+AGG + tig0023205:28310-28329 Msa1406210:intron 15.0%
!!! TTATATACTGAATAGATTTT+AGG + tig0023205:29297-29316 Msa1406210:intron 15.0%
!! CTTATAAACAAATATAAACT+TGG - tig0023205:30433-30452 None:intergenic 15.0%
!! CTAATTTATTAATTGTTAAC+AGG + tig0023205:30729-30748 Msa1406210:intron 15.0%
!! AATGAAATAGCAAAAAAATA+TGG + tig0023205:31276-31295 Msa1406210:CDS 15.0%
!! ATGAAATAGCAAAAAAATAT+GGG + tig0023205:31277-31296 Msa1406210:CDS 15.0%
!! TTCATATATAAATTAGAGAT+AGG + tig0023205:31486-31505 Msa1406210:three_prime_UTR 15.0%
!! CATATATATTCCAACAAAAA+AGG - tig0023205:27322-27341 None:intergenic 20.0%
!! AAAAATTGGTTAATGTATTG+TGG - tig0023205:27530-27549 None:intergenic 20.0%
!! TCTAGATAACAAGAAAAAAT+TGG - tig0023205:27544-27563 None:intergenic 20.0%
!!! GAATTTTGAAAATTATTCGA+GGG - tig0023205:27659-27678 None:intergenic 20.0%
!! CTAATTAGTACTAAATAACA+TGG - tig0023205:27681-27700 None:intergenic 20.0%
!!! TTAAGGATATTTCGTAATTT+TGG - tig0023205:28387-28406 None:intergenic 20.0%
!! GTTATTTATTCTATTATTGC+AGG + tig0023205:28626-28645 Msa1406210:intron 20.0%
!!! TCATTTTTCAATATATTCCT+AGG + tig0023205:28724-28743 Msa1406210:CDS 20.0%
!! AAATATAATGTATTCACTCT+TGG + tig0023205:29673-29692 Msa1406210:intron 20.0%
!! ATATAATCAATATAAATGGG+TGG + tig0023205:30294-30313 Msa1406210:CDS 20.0%
!! TATTATTCATCAGAAAAATC+AGG - tig0023205:31402-31421 None:intergenic 20.0%
!!! ATTAAACACAGTTTTTCAAA+TGG - tig0023205:31523-31542 None:intergenic 20.0%
! GGAATATTTCTCACTTTAAT+TGG + tig0023205:27287-27306 Msa1406210:five_prime_UTR 25.0%
! AGAAGAAAAAGCAACAAAAT+TGG - tig0023205:27352-27371 None:intergenic 25.0%
! ACACATTCTTGTTCAAATAT+CGG + tig0023205:27439-27458 Msa1406210:intron 25.0%
! TGAAGAGAAATTTGATTCTT+TGG + tig0023205:27578-27597 Msa1406210:five_prime_UTR 25.0%
! GAAGAGAAATTTGATTCTTT+GGG + tig0023205:27579-27598 Msa1406210:five_prime_UTR 25.0%
! AAAATTATTCGAGGGAATTT+AGG - tig0023205:27651-27670 None:intergenic 25.0%
!!! GGAATTTTGAAAATTATTCG+AGG - tig0023205:27660-27679 None:intergenic 25.0%
!! ATGTTTAGTTTCTTTGTTTG+TGG + tig0023205:28007-28026 Msa1406210:intron 25.0%
! ACATAAATAAACAGACTCAT+AGG - tig0023205:28305-28324 None:intergenic 25.0%
! TAGTACTTTGATAGACTAAA+CGG - tig0023205:28491-28510 None:intergenic 25.0%
! GATTATTATACCAACACTTA+GGG + tig0023205:28659-28678 Msa1406210:CDS 25.0%
! ATTATTATACCAACACTTAG+GGG + tig0023205:28660-28679 Msa1406210:CDS 25.0%
! GAAAAATGATGAAAAAACGA+AGG - tig0023205:28713-28732 None:intergenic 25.0%
! TTTACAATTTGTGATGAAAG+TGG - tig0023205:28765-28784 None:intergenic 25.0%
! CATGCTACAAAATAGTAAAA+GGG - tig0023205:28912-28931 None:intergenic 25.0%
! AGACTATTTCAAAGAGTTTA+CGG + tig0023205:29015-29034 Msa1406210:intron 25.0%
!!! TTAACTTGCTTATCCAAATT+TGG + tig0023205:29195-29214 Msa1406210:intron 25.0%
! GAGAAATCAAATTGTCAATA+TGG + tig0023205:29384-29403 Msa1406210:CDS 25.0%
!!! TAGAAGTACTTATTGAAGAA+TGG - tig0023205:29555-29574 None:intergenic 25.0%
! ATAATCAACTAGATAAAGCA+AGG - tig0023205:29637-29656 None:intergenic 25.0%
! GCTAAAAAATGGATATAATG+AGG + tig0023205:29698-29717 Msa1406210:intron 25.0%
! AGTATTAATACCTTTCTGTT+TGG + tig0023205:29730-29749 Msa1406210:intron 25.0%
!! TTTTCTCATACGATTCAATT+TGG - tig0023205:30117-30136 None:intergenic 25.0%
!! TTCAAGTTATATTAGAGGTA+TGG - tig0023205:30150-30169 None:intergenic 25.0%
!! CGAAATTCAAGTTATATTAG+AGG - tig0023205:30155-30174 None:intergenic 25.0%
! AATATATCAAAGATCGCTAT+GGG - tig0023205:30470-30489 None:intergenic 25.0%
! AAAAAAAGATATTCAGTCAG+AGG - tig0023205:30658-30677 None:intergenic 25.0%
! ATAGCAAAAAAATATGGGAA+AGG + tig0023205:31282-31301 Msa1406210:CDS 25.0%
!!! TTTTGTTGTTTATGGAACAA+GGG + tig0023205:31305-31324 Msa1406210:CDS 25.0%
!!! ATTGTGCAATTTGTTTGTTT+TGG + tig0023205:31455-31474 Msa1406210:three_prime_UTR 25.0%
ATATTCCAACAAAAAAGGGA+AGG - tig0023205:27317-27336 None:intergenic 30.0%
GTTACCATCATTGATATTTG+TGG + tig0023205:27504-27523 Msa1406210:intron 30.0%
! TTGATAATCCAAGATCTGAA+AGG + tig0023205:27618-27637 Msa1406210:CDS 30.0%
! GTGGGAATTTTTGATATTTC+AGG - tig0023205:27768-27787 None:intergenic 30.0%
!!! AGCATTCTTTTGAGACTTTT+TGG - tig0023205:27850-27869 None:intergenic 30.0%
AGTGTTAGTATTAGATCCTA+GGG + tig0023205:27944-27963 Msa1406210:intron 30.0%
! GAAGAAGTTTGCATTGATAT+TGG + tig0023205:28062-28081 Msa1406210:intron 30.0%
! TTATGTTTAAAAGGCCGTTT+TGG + tig0023205:28319-28338 Msa1406210:intron 30.0%
!!! TTCGTAATTTTGGCTAACTT+AGG - tig0023205:28377-28396 None:intergenic 30.0%
!! ATAACTAGAGACGCTTTTTA+AGG - tig0023205:28404-28423 None:intergenic 30.0%
TAAACGATCAAACTATCTAG+CGG - tig0023205:28451-28470 None:intergenic 30.0%
!! TATTATTGCAGGTGTTGATT+TGG + tig0023205:28637-28656 Msa1406210:intron 30.0%
GGATTATTATACCAACACTT+AGG + tig0023205:28658-28677 Msa1406210:CDS 30.0%
TCACAAATTGTAAAAGCTAC+AGG + tig0023205:28771-28790 Msa1406210:CDS 30.0%
CACAAATTGTAAAAGCTACA+GGG + tig0023205:28772-28791 Msa1406210:CDS 30.0%
ACAAATTGTAAAAGCTACAG+GGG + tig0023205:28773-28792 Msa1406210:CDS 30.0%
! TAGCTTGTGATATACTTACA+TGG - tig0023205:28860-28879 None:intergenic 30.0%
TATCACAAGCTATATTTCCT+TGG + tig0023205:28868-28887 Msa1406210:intron 30.0%
GCATGCTACAAAATAGTAAA+AGG - tig0023205:28913-28932 None:intergenic 30.0%
! TGTATTAGCTTATTCAAGCT+TGG + tig0023205:28971-28990 Msa1406210:intron 30.0%
AAGATATTATGGAGAGCTTA+TGG - tig0023205:29059-29078 None:intergenic 30.0%
!! ATTCTAGTTTAGGCCAAATT+TGG - tig0023205:29211-29230 None:intergenic 30.0%
AATTTATGTTCACCAAATGC+CGG + tig0023205:29474-29493 Msa1406210:CDS 30.0%
ATAAGTACTTCTATTGTCTC+TGG + tig0023205:29562-29581 Msa1406210:intron 30.0%
ATTAATACCTTTCTGTTTGG+AGG + tig0023205:29733-29752 Msa1406210:intron 30.0%
TTAATACCTTTCTGTTTGGA+GGG + tig0023205:29734-29753 Msa1406210:intron 30.0%
! CTTCTTACTAGCACTTTTAT+CGG + tig0023205:29778-29797 Msa1406210:CDS 30.0%
! CACTTTTATCGGAGAAATAA+TGG + tig0023205:29789-29808 Msa1406210:CDS 30.0%
! AGAAATAATGGTTGCTATTG+TGG + tig0023205:29801-29820 Msa1406210:CDS 30.0%
! CGTGGTTAATTTATTTCTCT+TGG + tig0023205:29879-29898 Msa1406210:intron 30.0%
AGAGAAATCATTTCTTACGT+TGG + tig0023205:29906-29925 Msa1406210:intron 30.0%
TTCTTACGTTGGATGAAATA+TGG + tig0023205:29917-29936 Msa1406210:intron 30.0%
!! ACCTTTCAAACCAGTTTTAT+AGG + tig0023205:29975-29994 Msa1406210:intron 30.0%
!! CCTTTCAAACCAGTTTTATA+GGG + tig0023205:29976-29995 Msa1406210:intron 30.0%
CTAATTCAACCCTATAAAAC+TGG - tig0023205:29988-30007 None:intergenic 30.0%
TCAAAAGCAGATATAAACGA+AGG - tig0023205:30053-30072 None:intergenic 30.0%
ATATATCAAAGATCGCTATG+GGG - tig0023205:30469-30488 None:intergenic 30.0%
GAATATATCAAAGATCGCTA+TGG - tig0023205:30471-30490 None:intergenic 30.0%
GAATTTGACTGTGATTTCTA+TGG - tig0023205:30493-30512 None:intergenic 30.0%
GAAATGCTCTTCATAATTAG+AGG + tig0023205:30865-30884 Msa1406210:CDS 30.0%
TTGTTGTGTATGAATCAAGA+TGG - tig0023205:30891-30910 None:intergenic 30.0%
CTTGATTCATACACAACAAA+TGG + tig0023205:30892-30911 Msa1406210:CDS 30.0%
TTCTTCAACTCATGTAGAAT+CGG + tig0023205:30928-30947 Msa1406210:CDS 30.0%
GACTACATAGTAAACAACTT+AGG + tig0023205:31112-31131 Msa1406210:CDS 30.0%
! TCAGATTTTATTCACACCAA+TGG + tig0023205:31142-31161 Msa1406210:CDS 30.0%
!!! GTTTTGTTGTTTATGGAACA+AGG + tig0023205:31304-31323 Msa1406210:CDS 30.0%
!! AAAGGTGTTAATATGCATTC+TGG + tig0023205:31345-31364 Msa1406210:CDS 30.0%
! AAGGTGTTAATATGCATTCT+GGG + tig0023205:31346-31365 Msa1406210:CDS 30.0%
! ATTCTTTGGGAAAACCAGTA+TGG + tig0023205:27592-27611 Msa1406210:exon 35.0%
! TTCTTTGGGAAAACCAGTAT+GGG + tig0023205:27593-27612 Msa1406210:exon 35.0%
TGGATTATCAAATCCCATAC+TGG - tig0023205:27609-27628 None:intergenic 35.0%
TCAAAAATTCCCACAACAAG+TGG + tig0023205:27774-27793 Msa1406210:CDS 35.0%
AAGGTGAAATACCACATTGA+TGG + tig0023205:27801-27820 Msa1406210:CDS 35.0%
GTATTTGTGTTCCATCAATG+TGG - tig0023205:27815-27834 None:intergenic 35.0%
!!! GACTTTTTGGAACTTTGTTC+AGG - tig0023205:27837-27856 None:intergenic 35.0%
AGTCTCAAAAGAATGCTACA+AGG + tig0023205:27853-27872 Msa1406210:CDS 35.0%
GAGTGTTAGTATTAGATCCT+AGG + tig0023205:27943-27962 Msa1406210:intron 35.0%
GTGTTAGTATTAGATCCTAG+GGG + tig0023205:27945-27964 Msa1406210:intron 35.0%
ACTGGCAAATAAAAGAACAG+TGG - tig0023205:28035-28054 None:intergenic 35.0%
!! ACTGTTCTTTTATTTGCCAG+TGG + tig0023205:28034-28053 Msa1406210:intron 35.0%
CTCACAGTTGTTAGATCATT+TGG + tig0023205:28104-28123 Msa1406210:intron 35.0%
CCTAAGAGCAATCTTAGAAT+AGG - tig0023205:28225-28244 None:intergenic 35.0%
!! CCTATTCTAAGATTGCTCTT+AGG + tig0023205:28222-28241 Msa1406210:CDS 35.0%
GCGTCTCTAGTTATTTAGTT+TGG + tig0023205:28410-28429 Msa1406210:intron 35.0%
ACTAACAAATGATACGCTCA+AGG + tig0023205:28507-28526 Msa1406210:intron 35.0%
CTAACAAATGATACGCTCAA+GGG + tig0023205:28508-28527 Msa1406210:intron 35.0%
! GACATTTTTCGTATTTGCCA+TGG - tig0023205:28691-28710 None:intergenic 35.0%
GGAAAAATCAAATACAGCCT+AGG - tig0023205:28744-28763 None:intergenic 35.0%
TTGTGATATACTTACATGGC+TGG - tig0023205:28856-28875 None:intergenic 35.0%
! CCAATTTGTTTGAACAACCA+AGG - tig0023205:28888-28907 None:intergenic 35.0%
CCTTGGTTGTTCAAACAAAT+TGG + tig0023205:28885-28904 Msa1406210:intron 35.0%
ATTTGGCCTAAACTAGAATG+TGG + tig0023205:29212-29231 Msa1406210:intron 35.0%
!! CTCATTCCACATTCTAGTTT+AGG - tig0023205:29221-29240 None:intergenic 35.0%
!! AATAGATTTTAGGAGCTTGC+TGG + tig0023205:29307-29326 Msa1406210:intron 35.0%
CTTGACGTTCTATTGATAGA+AGG - tig0023205:29335-29354 None:intergenic 35.0%
! TTGGAAAAGTGTGTGCAATA+TGG + tig0023205:29362-29381 Msa1406210:CDS 35.0%
TATACCAAACGGATAAAAGC+CGG - tig0023205:29496-29515 None:intergenic 35.0%
GCATCAGCATATATACCAAA+CGG - tig0023205:29507-29526 None:intergenic 35.0%
TTAACCATAATACTTGCCTC+AGG - tig0023205:29602-29621 None:intergenic 35.0%
CACTCTTGGAAGCTAAAAAA+TGG + tig0023205:29687-29706 Msa1406210:intron 35.0%
! ATTGCTGATTGGTAATATGC+AGG + tig0023205:29852-29871 Msa1406210:CDS 35.0%
!! CCCTATAAAACTGGTTTGAA+AGG - tig0023205:29979-29998 None:intergenic 35.0%
! GGGTTGAATTAGTTTGAAGA+TGG + tig0023205:29996-30015 Msa1406210:intron 35.0%
AAAGAACTGACACAGAACAA+TGG + tig0023205:30224-30243 Msa1406210:CDS 35.0%
TCATCCATTTGGTCAAACAA+GGG - tig0023205:30757-30776 None:intergenic 35.0%
TTCATCCATTTGGTCAAACA+AGG - tig0023205:30758-30777 None:intergenic 35.0%
CTAGCATTCTTTCATCCATT+TGG - tig0023205:30768-30787 None:intergenic 35.0%
GATTCATACACAACAAATGG+AGG + tig0023205:30895-30914 Msa1406210:CDS 35.0%
TAACTGTTCTTGTGGAAGAA+GGG - tig0023205:31020-31039 None:intergenic 35.0%
TTAACTGTTCTTGTGGAAGA+AGG - tig0023205:31021-31040 None:intergenic 35.0%
! GAAAGCTTTAACTGTTCTTG+TGG - tig0023205:31028-31047 None:intergenic 35.0%
!!! CTTGTTTTATTCAAGCTGCA+TGG + tig0023205:31178-31197 Msa1406210:CDS 35.0%
!!! GGGAAAGGTTTTGTTGTTTA+TGG + tig0023205:31297-31316 Msa1406210:CDS 35.0%
AGGTGTTAATATGCATTCTG+GGG + tig0023205:31347-31366 Msa1406210:CDS 35.0%
TCAGAAAAATCAGGTTCTGT+TGG - tig0023205:31393-31412 None:intergenic 35.0%
GTCACACTCCCCTTAAAATA+TGG - tig0023205:27156-27175 None:intergenic 40.0%
ATGAACTAGAAACCAGTTGG+AGG - tig0023205:27225-27244 None:intergenic 40.0%
CCTATGAACTAGAAACCAGT+TGG - tig0023205:27228-27247 None:intergenic 40.0%
!! CCAACTGGTTTCTAGTTCAT+AGG + tig0023205:27225-27244 Msa1406210:five_prime_UTR 40.0%
!! TGGTTTCTAGTTCATAGGTG+TGG + tig0023205:27230-27249 Msa1406210:five_prime_UTR 40.0%
!! TTTCTAGTTCATAGGTGTGG+AGG + tig0023205:27233-27252 Msa1406210:five_prime_UTR 40.0%
GTGGCCACAAATATCAATGA+TGG - tig0023205:27511-27530 None:intergenic 40.0%
!! ACCTTAACTCCACTTGTTGT+GGG - tig0023205:27786-27805 None:intergenic 40.0%
CAAACTTCTTCTCTAACCAC+TGG - tig0023205:28053-28072 None:intergenic 40.0%
TTATGTTGCTCCTTCTTCGA+AGG + tig0023205:28172-28191 Msa1406210:CDS 40.0%
CTACCAAAAACCTTCGAAGA+AGG - tig0023205:28185-28204 None:intergenic 40.0%
!!! TCTTCGAAGGTTTTTGGTAG+AGG + tig0023205:28185-28204 Msa1406210:CDS 40.0%
AGCACATTCAAGGACCAAAA+CGG - tig0023205:28336-28355 None:intergenic 40.0%
AGTGACACTGTCGTACATTT+AGG + tig0023205:28564-28583 Msa1406210:intron 40.0%
GGTTGTTACAGAAACAGCAT+GGG + tig0023205:28794-28813 Msa1406210:CDS 40.0%
CAACCTCATGCTTTACATGT+TGG + tig0023205:28830-28849 Msa1406210:CDS 40.0%
! TAGAACGTCAAGAAGCATGT+TGG + tig0023205:29343-29362 Msa1406210:CDS 40.0%
GCTATAAACCAAGCTACTCT+CGG - tig0023205:29441-29460 None:intergenic 40.0%
TGATGTAACTTCGGAAGTCA+TGG - tig0023205:29529-29548 None:intergenic 40.0%
GAATGGCGATGATGTAACTT+CGG - tig0023205:29538-29557 None:intergenic 40.0%
AGTACTTCTATTGTCTCTGG+TGG + tig0023205:29565-29584 Msa1406210:intron 40.0%
GTACTTCTATTGTCTCTGGT+GGG + tig0023205:29566-29585 Msa1406210:intron 40.0%
TACTTCTATTGTCTCTGGTG+GGG + tig0023205:29567-29586 Msa1406210:intron 40.0%
! GTACTTTTCGCATTGCTGAT+TGG + tig0023205:29841-29860 Msa1406210:CDS 40.0%
CGAAGGATGTATGTGTTTCA+CGG - tig0023205:30036-30055 None:intergenic 40.0%
GAGGAAGAAGATCTTCTCAA+AGG + tig0023205:30331-30350 Msa1406210:CDS 40.0%
ATTTCTCTCCTAAGATCCAG+CGG - tig0023205:30361-30380 None:intergenic 40.0%
GAACTAGTTCAAGACAAAGG+TGG - tig0023205:30390-30409 None:intergenic 40.0%
CTCGAACTAGTTCAAGACAA+AGG - tig0023205:30393-30412 None:intergenic 40.0%
CTTTGTCTTGAACTAGTTCG+AGG + tig0023205:30391-30410 Msa1406210:CDS 40.0%
TGATCTGGATCACAACATCA+AGG - tig0023205:30557-30576 None:intergenic 40.0%
AAAGATCTGCGACTATGATC+TGG - tig0023205:30572-30591 None:intergenic 40.0%
!! ATGAAGAGCATTTCGTTCAC+TGG - tig0023205:30859-30878 None:intergenic 40.0%
! ATGAGTTGAAGAATCCGTCA+CGG - tig0023205:30921-30940 None:intergenic 40.0%
TGGTGAAGAGCTCTTAACAT+GGG + tig0023205:30966-30985 Msa1406210:CDS 40.0%
ATTCACACCAATGGAGAACT+TGG + tig0023205:31151-31170 Msa1406210:CDS 40.0%
TTCACACCAATGGAGAACTT+GGG + tig0023205:31152-31171 Msa1406210:CDS 40.0%
TTATTCAAGCTGCATGGAGA+AGG + tig0023205:31184-31203 Msa1406210:CDS 40.0%
GAAAGAAGCTGCTGAATTGA+GGG + tig0023205:31218-31237 Msa1406210:CDS 40.0%
TTTATGGAACAAGGGTTGCA+AGG + tig0023205:31313-31332 Msa1406210:CDS 40.0%
GTTGCAAGGAATACTAGGAA+AGG + tig0023205:31327-31346 Msa1406210:CDS 40.0%
ACTATGCATGATGCACTTTG+TGG + tig0023205:31544-31563 Msa1406210:three_prime_UTR 40.0%
TATACTGCAACTCCTCCAAC+TGG + tig0023205:27210-27229 Msa1406210:five_prime_UTR 45.0%
GATAAGTTGCTTGCAGCAGA+GGG + tig0023205:27255-27274 Msa1406210:five_prime_UTR 45.0%
!!! GTGCTCCTTCCCTTTTTTGT+TGG + tig0023205:27309-27328 Msa1406210:five_prime_UTR 45.0%
! TCAATGATGGTAACGCGCAA+AGG - tig0023205:27498-27517 None:intergenic 45.0%
GAAGCACACCTTTCAGATCT+TGG - tig0023205:27629-27648 None:intergenic 45.0%
TCCCACAACAAGTGGAGTTA+AGG + tig0023205:27782-27801 Msa1406210:CDS 45.0%
! CACCTTAACTCCACTTGTTG+TGG - tig0023205:27787-27806 None:intergenic 45.0%
! GCTCCTTCTTCGAAGGTTTT+TGG + tig0023205:28179-28198 Msa1406210:CDS 45.0%
TGCTCTTAGGTACTTCAGCA+AGG + tig0023205:28235-28254 Msa1406210:CDS 45.0%
GCTCTTAGGTACTTCAGCAA+GGG + tig0023205:28236-28255 Msa1406210:CDS 45.0%
! GGTACTTCAGCAAGGGTTTT+TGG + tig0023205:28243-28262 Msa1406210:CDS 45.0%
AACAGACTCATAGGTACCTG+AGG - tig0023205:28296-28315 None:intergenic 45.0%
GACGACATCTAGCACATTCA+AGG - tig0023205:28346-28365 None:intergenic 45.0%
ATGGTTGAACCCCTAAGTGT+TGG - tig0023205:28672-28691 None:intergenic 45.0%
AACACTTAGGGGTTCAACCA+TGG + tig0023205:28671-28690 Msa1406210:CDS 45.0%
GGGTTGTTACAGAAACAGCA+TGG + tig0023205:28793-28812 Msa1406210:CDS 45.0%
TGGCCAACATGTAAAGCATG+AGG - tig0023205:28836-28855 None:intergenic 45.0%
AACGGATAAAAGCCGGCATT+TGG - tig0023205:29489-29508 None:intergenic 45.0%
!!! AATGCCGGCTTTTATCCGTT+TGG + tig0023205:29489-29508 Msa1406210:CDS 45.0%
ATTGTCTCTGGTGGGGATTA+AGG + tig0023205:29574-29593 Msa1406210:intron 45.0%
GGAACCTGAGGCAAGTATTA+TGG + tig0023205:29595-29614 Msa1406210:intron 45.0%
! TTTGGAGGGTGCAGTTCTTT+GGG + tig0023205:29748-29767 Msa1406210:intron 45.0%
GCTATTGTGGTTGCAACTCT+TGG + tig0023205:29814-29833 Msa1406210:CDS 45.0%
TGTGGTTGCAACTCTTGGAT+TGG + tig0023205:29819-29838 Msa1406210:CDS 45.0%
TGGTAATATGCAGGTAAGCG+TGG + tig0023205:29861-29880 Msa1406210:intron 45.0%
! GACGAACGGTTATCGATTGA+AGG - tig0023205:30186-30205 None:intergenic 45.0%
TAACCGTTCGTCTAGAAGAG+TGG + tig0023205:30194-30213 Msa1406210:CDS 45.0%
!! ATAGACTCTCGTAGTTCTGG+AGG - tig0023205:30268-30287 None:intergenic 45.0%
CGAATAGACTCTCGTAGTTC+TGG - tig0023205:30271-30290 None:intergenic 45.0%
TATAAATGGGTGGCTACACG+AGG + tig0023205:30304-30323 Msa1406210:CDS 45.0%
! GGTTCCCTTGTTTGACCAAA+TGG + tig0023205:30750-30769 Msa1406210:intron 45.0%
AAAGCCTGCATTATGCACTG+AGG + tig0023205:30807-30826 Msa1406210:CDS 45.0%
AAGCCTGCATTATGCACTGA+GGG + tig0023205:30808-30827 Msa1406210:CDS 45.0%
GGAACTTATCTTGTTCGCGA+AGG + tig0023205:30829-30848 Msa1406210:CDS 45.0%
AACTCATGTAGAATCGGTCC+AGG + tig0023205:30934-30953 Msa1406210:CDS 45.0%
GTGGTGAAGAGCTCTTAACA+TGG + tig0023205:30965-30984 Msa1406210:CDS 45.0%
!! CACACTTGGTCTTGGATCTA+AGG - tig0023205:30992-31011 None:intergenic 45.0%
!! GAAGAAGGGAGTATCACACT+TGG - tig0023205:31006-31025 None:intergenic 45.0%
! CATGGAGAAGGCACAAGAAA+AGG + tig0023205:31196-31215 Msa1406210:CDS 45.0%
GGAAAGAAGCTGCTGAATTG+AGG + tig0023205:31217-31236 Msa1406210:CDS 45.0%
CAAGGGTTGCAAGGAATACT+AGG + tig0023205:31322-31341 Msa1406210:CDS 45.0%
!! TAAATATAATCAATATAAAT+GGG + tig0023205:30291-30310 Msa1406210:CDS 5.0%
GGATAAGTTGCTTGCAGCAG+AGG + tig0023205:27254-27273 Msa1406210:five_prime_UTR 50.0%
TGCAGCAGAGGGAAATGCTT+TGG + tig0023205:27266-27285 Msa1406210:five_prime_UTR 50.0%
CGATGTATGAGTTGTCCCCT+AGG - tig0023205:27963-27982 None:intergenic 50.0%
GGGGACAACTCATACATCGT+TGG + tig0023205:27964-27983 Msa1406210:intron 50.0%
CTCATAGGTACCTGAGGAAG+AGG - tig0023205:28290-28309 None:intergenic 50.0%
GTTACAGAAACAGCATGGGC+AGG + tig0023205:28798-28817 Msa1406210:CDS 50.0%
AAGATGCTCCGAGAGTAGCT+TGG + tig0023205:29430-29449 Msa1406210:CDS 50.0%
ACTGCACCCTCCAAACAGAA+AGG - tig0023205:29743-29762 None:intergenic 50.0%
! GTTTGGAGGGTGCAGTTCTT+TGG + tig0023205:29747-29766 Msa1406210:intron 50.0%
CCTCCACTCTTCTAGACGAA+CGG - tig0023205:30200-30219 None:intergenic 50.0%
CGTTCGTCTAGAAGAGTGGA+GGG + tig0023205:30198-30217 Msa1406210:CDS 50.0%
TCTTCTCAAAGGACTTCCGC+TGG + tig0023205:30342-30361 Msa1406210:CDS 50.0%
AAGGACTTCCGCTGGATCTT+AGG + tig0023205:30350-30369 Msa1406210:CDS 50.0%
AGCTGAAGCAGCCACAATCA+CGG + tig0023205:30513-30532 Msa1406210:intron 50.0%
TGTCTCCACAACCGTGATTG+TGG - tig0023205:30527-30546 None:intergenic 50.0%
GTTCCCTCAGTGCATAATGC+AGG - tig0023205:30814-30833 None:intergenic 50.0%
ACAACAAATGGAGGCCGTGA+CGG + tig0023205:30904-30923 Msa1406210:CDS 50.0%
TCTTCACCACAGAAATCGCC+TGG - tig0023205:30955-30974 None:intergenic 50.0%
!! GGGAGTATCACACTTGGTCT+TGG - tig0023205:31000-31019 None:intergenic 50.0%
AAGCAGCCCAAGTTCTCCAT+TGG - tig0023205:31161-31180 None:intergenic 50.0%
AGCTGCTGAATTGAGGGCTA+AGG + tig0023205:31224-31243 Msa1406210:CDS 50.0%
GAGGGCTAAGGAGAATCTTG+TGG + tig0023205:31236-31255 Msa1406210:CDS 50.0%
GAATCTTGTGGCAGCTTCAG+AGG + tig0023205:31248-31267 Msa1406210:CDS 50.0%
! TGGTAACGCGCAAAGGCGAT+TGG - tig0023205:27491-27510 None:intergenic 55.0%
TGCTGCATTGCCTCTTCCTC+AGG + tig0023205:28277-28296 Msa1406210:CDS 55.0%
GGTGGGGATTAAGGAACCTG+AGG + tig0023205:29583-29602 Msa1406210:intron 55.0%
!! GGGTGCAGTTCTTTGGGACA+AGG + tig0023205:29754-29773 Msa1406210:intron 55.0%
CCGTTCGTCTAGAAGAGTGG+AGG + tig0023205:30197-30216 Msa1406210:CDS 55.0%
AGCAGCCACAATCACGGTTG+TGG + tig0023205:30519-30538 Msa1406210:intron 55.0%
ATCGGTCCAGGCGATTTCTG+TGG + tig0023205:30946-30965 Msa1406210:CDS 55.0%
GGTGGCTACACGAGGAGTAG+AGG + tig0023205:30312-30331 Msa1406210:CDS 60.0%
Chromosome Type Strat End Strand Name
tig0023205 gene 27152 31576 27152 ID=Msa1406210;Name=Msa1406210
tig0023205 mRNA 27152 31576 27152 ID=Msa1406210-mRNA-1;Parent=Msa1406210;Name=Msa1406210-mRNA-1;_AED=0.08;_eAED=0.08;_QI=237|0.75|0.66|1|0.87|0.77|9|157|614
tig0023205 exon 27152 27340 27152 ID=Msa1406210-mRNA-1:exon:24454;Parent=Msa1406210-mRNA-1
tig0023205 exon 27563 27639 27563 ID=Msa1406210-mRNA-1:exon:24455;Parent=Msa1406210-mRNA-1
tig0023205 exon 27752 27932 27752 ID=Msa1406210-mRNA-1:exon:24456;Parent=Msa1406210-mRNA-1
tig0023205 exon 28149 28298 28149 ID=Msa1406210-mRNA-1:exon:24457;Parent=Msa1406210-mRNA-1
tig0023205 exon 28648 28860 28648 ID=Msa1406210-mRNA-1:exon:24458;Parent=Msa1406210-mRNA-1
tig0023205 exon 29312 29565 29312 ID=Msa1406210-mRNA-1:exon:24459;Parent=Msa1406210-mRNA-1
tig0023205 exon 29762 29873 29762 ID=Msa1406210-mRNA-1:exon:24460;Parent=Msa1406210-mRNA-1
tig0023205 exon 30178 30414 30178 ID=Msa1406210-mRNA-1:exon:24461;Parent=Msa1406210-mRNA-1
tig0023205 exon 30751 31576 30751 ID=Msa1406210-mRNA-1:exon:24462;Parent=Msa1406210-mRNA-1
tig0023205 five_prime_UTR 27152 27340 27152 ID=Msa1406210-mRNA-1:five_prime_utr;Parent=Msa1406210-mRNA-1
tig0023205 five_prime_UTR 27563 27610 27563 ID=Msa1406210-mRNA-1:five_prime_utr;Parent=Msa1406210-mRNA-1
tig0023205 CDS 27611 27639 27611 ID=Msa1406210-mRNA-1:cds;Parent=Msa1406210-mRNA-1
tig0023205 CDS 27752 27932 27752 ID=Msa1406210-mRNA-1:cds;Parent=Msa1406210-mRNA-1
tig0023205 CDS 28149 28298 28149 ID=Msa1406210-mRNA-1:cds;Parent=Msa1406210-mRNA-1
tig0023205 CDS 28648 28860 28648 ID=Msa1406210-mRNA-1:cds;Parent=Msa1406210-mRNA-1
tig0023205 CDS 29312 29565 29312 ID=Msa1406210-mRNA-1:cds;Parent=Msa1406210-mRNA-1
tig0023205 CDS 29762 29873 29762 ID=Msa1406210-mRNA-1:cds;Parent=Msa1406210-mRNA-1
tig0023205 CDS 30178 30414 30178 ID=Msa1406210-mRNA-1:cds;Parent=Msa1406210-mRNA-1
tig0023205 CDS 30751 31419 30751 ID=Msa1406210-mRNA-1:cds;Parent=Msa1406210-mRNA-1
tig0023205 three_prime_UTR 31420 31576 31420 ID=Msa1406210-mRNA-1:three_prime_utr;Parent=Msa1406210-mRNA-1
Gene Sequence

>Msa1406210

ATGGGATTTGATAATCCAAGATCTGAAAGGTTTGAAGATGATCCTGAAATATCAAAAATTCCCACAACAAGTGGAGTTAAGGTGAAATACCACATTGATGGAACACAAATACCTGAACAAAGTTCCAAAAAGTCTCAAAAGAATGCTACAAGGAACAAGTTTTTGAAAACAAGAGTATTGTCAAGAGTATTCTCAGAAGACTATGAAAGAATTTGTATGAAGTTTCGTACAGCTTATGTTGCTCCTTCTTCGAAGGTTTTTGGTAGAGGAGAACTTGTTCTTACCTATTCTAAGATTGCTCTTAGGTACTTCAGCAAGGGTTTTTGGTTAGACTCTATTGCTGCATTGCCTCTTCCTCAGGTGTTGATTTGGATTATTATACCAACACTTAGGGGTTCAACCATGGCAAATACGAAAAATGTCCTTCGTTTTTTCATCATTTTTCAATATATTCCTAGGCTGTATTTGATTTTTCCACTTTCATCACAAATTGTAAAAGCTACAGGGGTTGTTACAGAAACAGCATGGGCAGGTGCTGCTTACAACCTCATGCTTTACATGTTGGCCAGCCATATTTTAGGAGCTTGCTGGTACCTTCTATCAATAGAACGTCAAGAAGCATGTTGGAAAAGTGTGTGCAATATGGAGAAATCAAATTGTCAATATGGATTCTTCAATTGTCACAGTGTTAAAGATGCTCCGAGAGTAGCTTGGTTTATAGCTAGTAATGTCACAAATTTATGTTCACCAAATGCCGGCTTTTATCCGTTTGGTATATATGCTGATGCCATGACTTCCGAAGTTACATCATCGCCATTCTTCAATAATTCTTTGGGACAAGGTCTTCTTACTAGCACTTTTATCGGAGAAATAATGGTTGCTATTGTGGTTGCAACTCTTGGATTGGTACTTTTCGCATTGCTGATTGGTAATATGCAGACATACCTTCAATCGATAACCGTTCGTCTAGAAGAGTGGAGGGTGAAAAGAACTGACACAGAACAATGGATGCATCACAGACAACTACCTCCAGAACTACGAGAGTCTATTCGTAAATATAATCAATATAAATGGGTGGCTACACGAGGAGTAGAGGAAGAAGATCTTCTCAAAGGACTTCCGCTGGATCTTAGGAGAGAAATCAAACGCCACCTTTGTCTTGAACTAGTTCGAGGAGTTCCCTTGTTTGACCAAATGGATGAAAGAATGCTAGACGCAATATGCGAAAGACTAAAGCCTGCATTATGCACTGAGGGAACTTATCTTGTTCGCGAAGGCGATCCAGTGAACGAAATGCTCTTCATAATTAGAGGCCATCTTGATTCATACACAACAAATGGAGGCCGTGACGGATTCTTCAACTCATGTAGAATCGGTCCAGGCGATTTCTGTGGTGAAGAGCTCTTAACATGGGCCTTAGATCCAAGACCAAGTGTGATACTCCCTTCTTCCACAAGAACAGTTAAAGCTTTCTCAGAAGTAGAAGCTTTTGCACTTATAGCTGAAGATCTTAAGTTTGTTGCATCTCAATTTAGAAGACTACATAGTAAACAACTTAGGCATAAATTCAGATTTTATTCACACCAATGGAGAACTTGGGCTGCTTGTTTTATTCAAGCTGCATGGAGAAGGCACAAGAAAAGGAAAGAAGCTGCTGAATTGAGGGCTAAGGAGAATCTTGTGGCAGCTTCAGAGGCTGAAAATGAAATAGCAAAAAAATATGGGAAAGGTTTTGTTGTTTATGGAACAAGGGTTGCAAGGAATACTAGGAAAGGTGTTAATATGCATTCTGGGGTAGTAAGCTCTTTGCAGAAACCAACAGAACCTGATTTTTCTGATGAATAA
Protein sequence

>Msa1406210

MGFDNPRSERFEDDPEISKIPTTSGVKVKYHIDGTQIPEQSSKKSQKNATRNKFLKTRVLSRVFSEDYERICMKFRTAYVAPSSKVFGRGELVLTYSKIALRYFSKGFWLDSIAALPLPQVLIWIIIPTLRGSTMANTKNVLRFFIIFQYIPRLYLIFPLSSQIVKATGVVTETAWAGAAYNLMLYMLASHILGACWYLLSIERQEACWKSVCNMEKSNCQYGFFNCHSVKDAPRVAWFIASNVTNLCSPNAGFYPFGIYADAMTSEVTSSPFFNNSLGQGLLTSTFIGEIMVAIVVATLGLVLFALLIGNMQTYLQSITVRLEEWRVKRTDTEQWMHHRQLPPELRESIRKYNQYKWVATRGVEEEDLLKGLPLDLRREIKRHLCLELVRGVPLFDQMDERMLDAICERLKPALCTEGTYLVREGDPVNEMLFIIRGHLDSYTTNGGRDGFFNSCRIGPGDFCGEELLTWALDPRPSVILPSSTRTVKAFSEVEAFALIAEDLKFVASQFRRLHSKQLRHKFRFYSHQWRTWAACFIQAAWRRHKKRKEAAELRAKENLVAASEAENEIAKKYGKGFVVYGTRVARNTRKGVNMHSGVVSSLQKPTEPDFSDE*