Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0011660 | Msa0270530 | -0.808669 | 2.764678e-50 | -8.615850e-47 |
| Msa0150840 | Msa0270530 | 0.816692 | 4.850743e-52 | -8.615850e-47 |
| Msa0259370 | Msa0270530 | 0.806622 | 7.522658e-50 | -8.615850e-47 |
| Msa0270530 | Msa0326480 | 0.808567 | 2.907329e-50 | -8.615850e-47 |
| Msa0270530 | Msa1254730 | 0.812481 | 4.148063e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0270530 | MtrunA17_Chr1g0179481 | 66.667 | 42 | 11 | 1 | 8 | 46 | 1 | 42 | 2.96e-11 | 53.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTTACCAGAAGTGAATTTC+TGG | 0.230498 | 2_3:+1737680 | Msa0270530:CDS |
| GTGGAATGGATGAAGTTTCT+TGG | 0.346424 | 2_3:+1737601 | Msa0270530:CDS |
| ATCGCCGGCGATGGCGGATC+TGG | 0.403965 | 2_3:+1737338 | Msa0270530:CDS |
| AAAACCCAGAAATTCACTTC+TGG | 0.415665 | 2_3:-1737685 | None:intergenic |
| GATGAACGACAGTGACGATA+TGG | 0.452933 | 2_3:+1737263 | Msa0270530:CDS |
| GTTGTGGCCATCGCCGGCGA+TGG | 0.465234 | 2_3:+1737329 | Msa0270530:CDS |
| TTACAAGAAGATAAGTTTGT+GGG | 0.479052 | 2_3:+1737706 | Msa0270530:CDS |
| AGCCGCGTTGTGGCCATCGC+CGG | 0.479852 | 2_3:+1737323 | Msa0270530:CDS |
| TTTACCAGAAGTGAATTTCT+GGG | 0.486028 | 2_3:+1737681 | Msa0270530:CDS |
| CGGATCTGGTGGCTAGTGAA+TGG | 0.491380 | 2_3:+1737352 | Msa0270530:CDS |
| GCTACCATGTGCACCACCGC+TGG | 0.509339 | 2_3:-1737379 | None:intergenic |
| GCCACCAGATCCGCCATCGC+CGG | 0.510994 | 2_3:-1737342 | None:intergenic |
| GGATCTGGTGGCTAGTGAAT+GGG | 0.517610 | 2_3:+1737353 | Msa0270530:CDS |
| TACTATTGTTTCTCTCTGTG+TGG | 0.529996 | 2_3:+1737657 | Msa0270530:CDS |
| AGATCCGCCATCGCCGGCGA+TGG | 0.541049 | 2_3:-1737336 | None:intergenic |
| GACGCCAGCGGTGGTGCACA+TGG | 0.546341 | 2_3:+1737375 | Msa0270530:CDS |
| TTTACAAGAAGATAAGTTTG+TGG | 0.552537 | 2_3:+1737705 | Msa0270530:CDS |
| GTGGCCATCGCCGGCGATGG+CGG | 0.553562 | 2_3:+1737332 | Msa0270530:CDS |
| GCCGGCGATGGCGGATCTGG+TGG | 0.565813 | 2_3:+1737341 | Msa0270530:CDS |
| GAATGAGAACAGCCGCGTTG+TGG | 0.576381 | 2_3:+1737313 | Msa0270530:CDS |
| AGTGAATGGGACGCCAGCGG+TGG | 0.581524 | 2_3:+1737366 | Msa0270530:CDS |
| AATTGGTGTGGATTGTGGAA+TGG | 0.581574 | 2_3:+1737587 | Msa0270530:CDS |
| TGGAAGAGAGTCGTCTGAGA+CGG | 0.603786 | 2_3:+1737289 | Msa0270530:CDS |
| CGCCGGCGATGGCCACAACG+CGG | 0.622562 | 2_3:-1737325 | None:intergenic |
| GCTAGTGAATGGGACGCCAG+CGG | 0.639122 | 2_3:+1737363 | Msa0270530:CDS |
| CGACAGTGACGATATGGTGA+TGG | 0.647895 | 2_3:+1737269 | Msa0270530:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAAAAAAAAATCACTTCTTT+CGG | - | chr2_3:1737453-1737472 | None:intergenic | 20.0% |
| !! | TGTTTGTTTGAAAGAAAAAT+TGG | + | chr2_3:1737570-1737589 | Msa0270530:intron | 20.0% |
| ! | AGCAAAGTAAAACAAATTCA+AGG | - | chr2_3:1737475-1737494 | None:intergenic | 25.0% |
| !!! | TTTTACTTTGCTGTTAGTTT+TGG | + | chr2_3:1737483-1737502 | Msa0270530:intron | 25.0% |
| !! | TTTGTTATGAATTGAGTTCA+TGG | + | chr2_3:1737534-1737553 | Msa0270530:intron | 25.0% |
| ! | TTTACAAGAAGATAAGTTTG+TGG | + | chr2_3:1737705-1737724 | Msa0270530:CDS | 25.0% |
| ! | TTACAAGAAGATAAGTTTGT+GGG | + | chr2_3:1737706-1737725 | Msa0270530:CDS | 25.0% |
| !! | GTCTAAAGAGTATTAAGTTG+CGG | + | chr2_3:1737413-1737432 | Msa0270530:intron | 30.0% |
| !!! | AGTTTTGGTTTAGAATGTGT+TGG | + | chr2_3:1737498-1737517 | Msa0270530:intron | 30.0% |
| GTTTGAAAGAAAAATTGGTG+TGG | + | chr2_3:1737575-1737594 | Msa0270530:intron | 30.0% | |
| ATTTACCAGAAGTGAATTTC+TGG | + | chr2_3:1737680-1737699 | Msa0270530:CDS | 30.0% | |
| TTTACCAGAAGTGAATTTCT+GGG | + | chr2_3:1737681-1737700 | Msa0270530:CDS | 30.0% | |
| !! | TAAAGAGTATTAAGTTGCGG+TGG | + | chr2_3:1737416-1737435 | Msa0270530:intron | 35.0% |
| ! | AGAAAAATTGGTGTGGATTG+TGG | + | chr2_3:1737582-1737601 | Msa0270530:intron | 35.0% |
| ! | TACTATTGTTTCTCTCTGTG+TGG | + | chr2_3:1737657-1737676 | Msa0270530:CDS | 35.0% |
| AAAACCCAGAAATTCACTTC+TGG | - | chr2_3:1737688-1737707 | None:intergenic | 35.0% | |
| ! | AATTGGTGTGGATTGTGGAA+TGG | + | chr2_3:1737587-1737606 | Msa0270530:CDS | 40.0% |
| GTGGAATGGATGAAGTTTCT+TGG | + | chr2_3:1737601-1737620 | Msa0270530:CDS | 40.0% | |
| GATGAACGACAGTGACGATA+TGG | + | chr2_3:1737263-1737282 | Msa0270530:CDS | 45.0% | |
| CGACAGTGACGATATGGTGA+TGG | + | chr2_3:1737269-1737288 | Msa0270530:CDS | 50.0% | |
| TGGAAGAGAGTCGTCTGAGA+CGG | + | chr2_3:1737289-1737308 | Msa0270530:CDS | 50.0% | |
| GGATCTGGTGGCTAGTGAAT+GGG | + | chr2_3:1737353-1737372 | Msa0270530:CDS | 50.0% | |
| GAATGAGAACAGCCGCGTTG+TGG | + | chr2_3:1737313-1737332 | Msa0270530:CDS | 55.0% | |
| CGGATCTGGTGGCTAGTGAA+TGG | + | chr2_3:1737352-1737371 | Msa0270530:CDS | 55.0% | |
| GCTAGTGAATGGGACGCCAG+CGG | + | chr2_3:1737363-1737382 | Msa0270530:CDS | 60.0% | |
| AGTGAATGGGACGCCAGCGG+TGG | + | chr2_3:1737366-1737385 | Msa0270530:CDS | 65.0% | |
| GCTACCATGTGCACCACCGC+TGG | - | chr2_3:1737382-1737401 | None:intergenic | 65.0% | |
| ! | GTGGTGCACATGGTAGCGCG+AGG | + | chr2_3:1737385-1737404 | Msa0270530:intron | 65.0% |
| AGCCGCGTTGTGGCCATCGC+CGG | + | chr2_3:1737323-1737342 | Msa0270530:CDS | 70.0% | |
| GTTGTGGCCATCGCCGGCGA+TGG | + | chr2_3:1737329-1737348 | Msa0270530:CDS | 70.0% | |
| AGATCCGCCATCGCCGGCGA+TGG | - | chr2_3:1737339-1737358 | None:intergenic | 70.0% | |
| !! | ATCGCCGGCGATGGCGGATC+TGG | + | chr2_3:1737338-1737357 | Msa0270530:CDS | 70.0% |
| GCCACCAGATCCGCCATCGC+CGG | - | chr2_3:1737345-1737364 | None:intergenic | 70.0% | |
| GACGCCAGCGGTGGTGCACA+TGG | + | chr2_3:1737375-1737394 | Msa0270530:CDS | 70.0% | |
| CGCCGGCGATGGCCACAACG+CGG | - | chr2_3:1737328-1737347 | None:intergenic | 75.0% | |
| GTGGCCATCGCCGGCGATGG+CGG | + | chr2_3:1737332-1737351 | Msa0270530:CDS | 75.0% | |
| !! | GCCGGCGATGGCGGATCTGG+TGG | + | chr2_3:1737341-1737360 | Msa0270530:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_3 | gene | 1737261 | 1737750 | 1737261 | ID=Msa0270530;Name=Msa0270530 |
| chr2_3 | mRNA | 1737261 | 1737750 | 1737261 | ID=Msa0270530-mRNA-1;Parent=Msa0270530;Name=Msa0270530-mRNA-1;_AED=0.16;_eAED=0.32;_QI=0|0|0|1|0|0.5|2|0|100 |
| chr2_3 | exon | 1737261 | 1737396 | 1737261 | ID=Msa0270530-mRNA-1:exon:788;Parent=Msa0270530-mRNA-1 |
| chr2_3 | exon | 1737584 | 1737750 | 1737584 | ID=Msa0270530-mRNA-1:exon:789;Parent=Msa0270530-mRNA-1 |
| chr2_3 | CDS | 1737261 | 1737396 | 1737261 | ID=Msa0270530-mRNA-1:cds;Parent=Msa0270530-mRNA-1 |
| chr2_3 | CDS | 1737584 | 1737750 | 1737584 | ID=Msa0270530-mRNA-1:cds;Parent=Msa0270530-mRNA-1 |
| Gene Sequence |
| Protein sequence |
