AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0272910

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0272910 MtrunA17_Chr3g0138401 88.542 96 8 1 96 188 321 416 1.16e-50 168
Msa0272910 MtrunA17_Chr1g0198231 96.774 31 1 0 61 91 291 321 2.14e-15 73.2
Msa0272910 MtrunA17_Chr8g0367631 93.548 31 2 0 61 91 281 311 1.24e-14 70.9
Msa0272910 MtrunA17_Chr7g0265591 100.000 30 0 0 32 61 262 291 1.61e-13 68.2
Msa0272910 MtrunA17_Chr1g0179541 100.000 30 0 0 32 61 246 275 2.14e-13 67.8
Msa0272910 MtrunA17_Chr2g0278901 80.645 31 6 0 61 91 284 314 2.59e-12 64.3
Msa0272910 MtrunA17_Chr1g0197911 90.000 30 3 0 32 61 261 290 1.13e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0272910 AT3G52990.2 100.000 30 0 0 32 61 209 238 2.54e-13 67.8
Msa0272910 AT3G52990.1 100.000 30 0 0 32 61 262 291 2.63e-13 67.8
Msa0272910 AT2G36580.1 100.000 30 0 0 32 61 262 291 2.98e-13 67.8
Msa0272910 AT1G80670.1 80.645 31 6 0 61 91 282 312 4.07e-12 63.9

Find 63 sgRNAs with CRISPR-Local

Find 247 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCTTGTCCCGTGGGAATT+TGG 0.131046 2_3:-4480132 Msa0272910:CDS
TGCTTGAACAGCCAGATTTC+TGG 0.202017 2_3:+4477773 None:intergenic
TCTAAACCGGTCACCTTATC+AGG 0.216944 2_3:-4477466 Msa0272910:three_prime_UTR
CTGTGTCTAGGTGCAGGGTC+TGG 0.269441 2_3:-4480483 Msa0272910:intron
CTTGTAGAATCTCATCAAAA+TGG 0.282372 2_3:+4480168 None:intergenic
ACCAGCTTTGCAGTGGATTT+AGG 0.291769 2_3:+4478447 None:intergenic
ATCTTGTCCCGTGGGAATTT+GGG 0.295886 2_3:-4480131 Msa0272910:CDS
GCATACCGCGTAAGCATATA+TGG 0.299683 2_3:+4478896 None:intergenic
TGCTAAAGATACCAGAAATC+TGG 0.323635 2_3:-4477784 Msa0272910:CDS
GTCTACAGGTTTGTTATGAT+TGG 0.341574 2_3:-4478515 Msa0272910:intron
TTAGCTTATTCCTCTTTGAT+AGG 0.342074 2_3:-4480419 Msa0272910:CDS
CATAGTGGAGAAAATGAAAC+AGG 0.343306 2_3:-4478408 Msa0272910:CDS
CAGAAATAAGAGAAAGCTCT+TGG 0.383667 2_3:+4477666 None:intergenic
TTCTATTGCAGTTTCTAAAC+CGG 0.384539 2_3:-4477479 Msa0272910:three_prime_UTR
TGTGTCTAGGTGCAGGGTCT+GGG 0.392512 2_3:-4480482 Msa0272910:intron
CATGGGAAGTTGTCTGCTAC+TGG 0.416162 2_3:-4478360 Msa0272910:CDS
ATTGAATGTACTGCAGGGTA+TGG 0.417856 2_3:+4478929 None:intergenic
ACAAAGAATCTAACCTTCTC+TGG 0.426853 2_3:+4480095 None:intergenic
GCCTAAATCCACTGCAAAGC+TGG 0.430607 2_3:-4478448 Msa0272910:CDS
TCCTGCTGTGTCTAGGTGCA+GGG 0.434688 2_3:-4480488 Msa0272910:intron
AGAAGATGCTTGACCTGATA+AGG 0.443032 2_3:+4477453 None:intergenic
GTCATTATTGAATGTACTGC+AGG 0.456046 2_3:+4478923 None:intergenic
TTAAACATGTTGCCATTTGT+TGG 0.456841 2_3:-4480453 Msa0272910:CDS
GGAGTAGATACACGATCAAC+AGG 0.468170 2_3:+4478315 None:intergenic
TTGTAGAATCTCATCAAAAT+GGG 0.469141 2_3:+4480169 None:intergenic
ACTGCAAAGATGGTGGATCT+TGG 0.479375 2_3:-4478485 Msa0272910:CDS
GTGAGAAAATTTGACAAAAG+AGG 0.485887 2_3:+4477624 None:intergenic
AGAAATAAGAGAAAGCTCTT+GGG 0.490918 2_3:+4477667 None:intergenic
AGTAATCCTCAAGAGCTTCA+TGG 0.497229 2_3:-4478378 Msa0272910:CDS
TTATTCCTCTTTGATAGGAG+TGG 0.500496 2_3:-4480414 Msa0272910:CDS
ATGTTTCTGTAATCAATGGC+AGG 0.504577 2_3:-4481207 None:intergenic
GAGTAGGATGATTGTTAAAG+AGG 0.509074 2_3:-4477556 Msa0272910:three_prime_UTR
AACTTCCCATGAAGCTCTTG+AGG 0.518605 2_3:+4478372 None:intergenic
ATTATGTGGGGAGCAAGAGT+AGG 0.524042 2_3:-4477572 Msa0272910:three_prime_UTR
GGCAGTTCTAGCCGTGCCGT+TGG 0.525116 2_3:-4478339 Msa0272910:CDS
GTAATCCTCAAGAGCTTCAT+GGG 0.525373 2_3:-4478377 Msa0272910:CDS
AATAACAACCTTACGAATGT+TGG 0.526846 2_3:-4477418 Msa0272910:three_prime_UTR
AATGTTGCTTCACCAACAAA+TGG 0.529121 2_3:+4480441 None:intergenic
CATTGATCTTCCACCAGAGA+AGG 0.538672 2_3:-4480108 Msa0272910:intron
ATCATTGACCAGCTTTGCAG+TGG 0.539773 2_3:+4478440 None:intergenic
TGGAGCAACTACTGCAAAGA+TGG 0.553115 2_3:-4478495 Msa0272910:CDS
TCTACTTACCAACATTCGTA+AGG 0.553647 2_3:+4477410 None:intergenic
GCTTGACCTGATAAGGTGAC+CGG 0.558454 2_3:+4477460 None:intergenic
AATGATAGTAACTTGCATAG+TGG 0.579416 2_3:-4478423 Msa0272910:CDS
GAGATTCTACAAGAAGCTGA+TGG 0.579505 2_3:-4480158 Msa0272910:CDS
CCTCTTTGATAGGAGTGGTG+TGG 0.581690 2_3:-4480409 Msa0272910:intron
GATCGTGTATCTACTCCAGC+CGG 0.597972 2_3:-4478309 Msa0272910:intron
TACACGATCAACAGGACCAA+CGG 0.602833 2_3:+4478323 None:intergenic
ATGGAATCATCTTGTCCCGT+GGG 0.603759 2_3:-4480139 Msa0272910:CDS
TAATTGATGTATCTGTTAGA+CGG 0.604298 2_3:+4477532 None:intergenic
TGCAGTACATTCAATAATGA+CGG 0.604536 2_3:-4478921 Msa0272910:CDS
CCACACCACTCCTATCAAAG+AGG 0.605958 2_3:+4480409 None:intergenic
AAGAATCTAACCTTCTCTGG+TGG 0.606089 2_3:+4480098 None:intergenic
ATCAATGCCCAAATTCCCAC+GGG 0.610460 2_3:+4480124 None:intergenic
TGTATTGTAGGGGCACATCA+AGG 0.610537 2_3:-4477816 Msa0272910:intron
TTGAATGTACTGCAGGGTAT+GGG 0.618328 2_3:+4478930 None:intergenic
AGCAACTACTGCAAAGATGG+TGG 0.619072 2_3:-4478492 Msa0272910:CDS
GATCAATGCCCAAATTCCCA+CGG 0.634784 2_3:+4480123 None:intergenic
GATCAACAGGACCAACGGCA+CGG 0.637608 2_3:+4478328 None:intergenic
GATGGAATCATCTTGTCCCG+TGG 0.645597 2_3:-4480140 Msa0272910:CDS
TCATTATTGAATGTACTGCA+GGG 0.646171 2_3:+4478924 None:intergenic
CGGTTCCATATATGCTTACG+CGG 0.673675 2_3:-4478901 Msa0272910:intron
ATAGTGGAGAAAATGAAACA+GGG 0.722474 2_3:-4478407 Msa0272910:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAGAAAAAATTTGATTTTTT+TGG - chr2_3:4477725-4477744 Msa0272910:exon 10.0%
!! TACTTAATAATTCTTAAAAT+GGG - chr2_3:4478550-4478569 Msa0272910:intron 10.0%
!!! CATAATCTTTTTTTTTTTTA+AGG + chr2_3:4480957-4480976 None:intergenic 10.0%
!! TAAAAAAAAAAAAGATTATG+TGG - chr2_3:4480957-4480976 Msa0272910:intron 10.0%
!! AAAAAAAAAAAAGATTATGT+GGG - chr2_3:4480958-4480977 Msa0272910:intron 10.0%
!!! AAATTTGATTTTTTTGGATT+TGG - chr2_3:4477731-4477750 Msa0272910:exon 15.0%
!!! TTTATATATCTGTTCTTTTT+AGG - chr2_3:4478345-4478364 Msa0272910:CDS 15.0%
!!! TTATATATCTGTTCTTTTTA+GGG - chr2_3:4478346-4478365 Msa0272910:CDS 15.0%
!! GTACTTAATAATTCTTAAAA+TGG - chr2_3:4478549-4478568 Msa0272910:intron 15.0%
!! ACTTAATAATTCTTAAAATG+GGG - chr2_3:4478551-4478570 Msa0272910:intron 15.0%
!!! ATATTTTTGTTTGTTTTATG+AGG - chr2_3:4478581-4478600 Msa0272910:intron 15.0%
!!! TTAATCTCAAATTTTTTCTT+TGG - chr2_3:4478688-4478707 Msa0272910:intron 15.0%
!!! TATCTAATCTAAGAATTTTT+AGG - chr2_3:4479179-4479198 Msa0272910:intron 15.0%
!! TTCTAAATTCATTAGAATAT+TGG - chr2_3:4480539-4480558 Msa0272910:intron 15.0%
!! AATCAATAAAATATTAGAGT+TGG + chr2_3:4480595-4480614 None:intergenic 15.0%
!!! TTTTTATGTTCATATTCAAT+GGG - chr2_3:4480850-4480869 Msa0272910:intron 15.0%
!! AAAAAAAAAAAGATTATGTG+GGG - chr2_3:4480959-4480978 Msa0272910:intron 15.0%
!!! AGAATGAATATAACAATTTT+TGG - chr2_3:4481172-4481191 Msa0272910:intron 15.0%
!! TAGGAAAGATTGAATAAAAT+TGG + chr2_3:4478631-4478650 None:intergenic 20.0%
!! AAGGCAAATAAATAAATCTT+AGG + chr2_3:4478812-4478831 None:intergenic 20.0%
!! AAGATTTATTTATTTGCCTT+TGG - chr2_3:4478812-4478831 Msa0272910:intron 20.0%
!! AAACCTTAACATAGTAATTA+TGG + chr2_3:4478852-4478871 None:intergenic 20.0%
!!! TTACTATGTTAAGGTTTTAT+TGG - chr2_3:4478855-4478874 Msa0272910:intron 20.0%
!!! TTTTATATACATGATCATCA+TGG - chr2_3:4478881-4478900 Msa0272910:intron 20.0%
!! ATTTCTCTTCATTATTCATT+AGG + chr2_3:4479146-4479165 None:intergenic 20.0%
!! AATAACAGACAAAACTTTAT+CGG + chr2_3:4479311-4479330 None:intergenic 20.0%
!! ATCCTTTCAATATAAGATAT+GGG - chr2_3:4479421-4479440 Msa0272910:intron 20.0%
!! TTAATAGTGTACAGTAATTA+CGG + chr2_3:4479476-4479495 None:intergenic 20.0%
!!! TATTTTCTAGATTTGATACA+TGG + chr2_3:4479499-4479518 None:intergenic 20.0%
!! ACATAGAAAGTAAATAACTA+CGG + chr2_3:4479694-4479713 None:intergenic 20.0%
!!! ATAATTTGCTTGCTATTTTA+TGG - chr2_3:4479941-4479960 Msa0272910:intron 20.0%
!! AATTTCCCTTAATATGATAT+CGG - chr2_3:4480386-4480405 Msa0272910:intron 20.0%
!! TCTAATGAATTTAGAAAGAA+AGG + chr2_3:4480535-4480554 None:intergenic 20.0%
!!! ATAAAATATTAGAGTTGGTA+CGG + chr2_3:4480590-4480609 None:intergenic 20.0%
!!! GTTTTTATGTTCATATTCAA+TGG - chr2_3:4480849-4480868 Msa0272910:intron 20.0%
!!! ACAATTTTTGGTTTAAAAAG+AGG - chr2_3:4481184-4481203 Msa0272910:intron 20.0%
!! ATGACTCAAATTTTCTGATA+TGG - chr2_3:4477361-4477380 Msa0272910:three_prime_UTR 25.0%
!!! TCTTCTTTTAGGCTTTAATT+TGG - chr2_3:4477590-4477609 Msa0272910:three_prime_UTR 25.0%
!! ATTTAGTGTTATGCTCTAAA+TGG - chr2_3:4477640-4477659 Msa0272910:three_prime_UTR 25.0%
!! TTTAGTGTTATGCTCTAAAT+GGG - chr2_3:4477641-4477660 Msa0272910:three_prime_UTR 25.0%
! AAAAACAGGAAAATAAATGC+AGG + chr2_3:4477760-4477779 None:intergenic 25.0%
!!! TGTTTTTTTATCGTTTTTGC+TGG - chr2_3:4478025-4478044 Msa0272910:intron 25.0%
!!! GTTTTTTTATCGTTTTTGCT+GGG - chr2_3:4478026-4478045 Msa0272910:intron 25.0%
! TCATGTTATATAGTGTTGTA+GGG - chr2_3:4478191-4478210 Msa0272910:intron 25.0%
! GACACTTATAATTCTACATT+AGG - chr2_3:4478213-4478232 Msa0272910:intron 25.0%
!! ATCTAAGATTGAAAAGATTG+AGG - chr2_3:4478238-4478257 Msa0272910:intron 25.0%
! TTGTAGAATCTCATCAAAAT+GGG + chr2_3:4478377-4478396 None:intergenic 25.0%
! ATAATTCTTAAAATGGGGTT+TGG - chr2_3:4478556-4478575 Msa0272910:intron 25.0%
! ATATTGAGACCATACTTTAT+TGG - chr2_3:4478769-4478788 Msa0272910:intron 25.0%
! ACATAGTAATTATGGACATT+TGG + chr2_3:4478844-4478863 None:intergenic 25.0%
! TGTCCATAATTACTATGTTA+AGG - chr2_3:4478846-4478865 Msa0272910:intron 25.0%
!! TCACTCTTTTACAGTATAAA+GGG + chr2_3:4478985-4479004 None:intergenic 25.0%
!! TTCACTCTTTTACAGTATAA+AGG + chr2_3:4478986-4479005 None:intergenic 25.0%
!! TACACCTAATTTTTATTCCT+GGG - chr2_3:4479205-4479224 Msa0272910:intron 25.0%
! TCACTTCATAGTAAAATTGA+AGG - chr2_3:4479395-4479414 Msa0272910:intron 25.0%
! GATCCTTTCAATATAAGATA+TGG - chr2_3:4479420-4479439 Msa0272910:intron 25.0%
!!! ATTTTTTGTAGAATATGCAG+AGG - chr2_3:4479581-4479600 Msa0272910:intron 25.0%
!!! TTTGTTTCTAGCTTTATAGT+TGG - chr2_3:4479730-4479749 Msa0272910:intron 25.0%
! AATTATGGTAACTGAATTGA+AGG + chr2_3:4479982-4480001 None:intergenic 25.0%
! GTATTATAATACCTCCAAAT+TGG - chr2_3:4480309-4480328 Msa0272910:intron 25.0%
!! TGATTGTTCAAAGCTAATTT+TGG + chr2_3:4480432-4480451 None:intergenic 25.0%
!!! TAAAATATTAGAGTTGGTAC+GGG + chr2_3:4480589-4480608 None:intergenic 25.0%
!! AGAAGACAAGAATTTTCATA+TGG + chr2_3:4480634-4480653 None:intergenic 25.0%
! TGAAAATTCTTGTCTTCTTT+AGG - chr2_3:4480636-4480655 Msa0272910:intron 25.0%
! TAATTGATGTATCTGTTAGA+CGG + chr2_3:4481014-4481033 None:intergenic 25.0%
!!! GCAATAGAAGATGTTTTTTA+CGG + chr2_3:4481053-4481072 None:intergenic 25.0%
! TTCAATTAGATAGAGTGCTT+TGG - chr2_3:4477416-4477435 Msa0272910:three_prime_UTR 30.0%
ATGTAACTGAAACCTTGATT+CGG - chr2_3:4477485-4477504 Msa0272910:three_prime_UTR 30.0%
! ATGCTTCAAAGGCTTTAATT+TGG - chr2_3:4477530-4477549 Msa0272910:three_prime_UTR 30.0%
! TGCTTCAAAGGCTTTAATTT+GGG - chr2_3:4477531-4477550 Msa0272910:three_prime_UTR 30.0%
!! CAAGTTGAGTTTCTTCTTTT+AGG - chr2_3:4477579-4477598 Msa0272910:three_prime_UTR 30.0%
!! AGGCTTTAATTTGGTTGTAT+AGG - chr2_3:4477599-4477618 Msa0272910:three_prime_UTR 30.0%
GAATTGTTGTCTATGAACTT+GGG - chr2_3:4477663-4477682 Msa0272910:three_prime_UTR 30.0%
TCTGCAATCACATAAAAAAC+AGG + chr2_3:4477774-4477793 None:intergenic 30.0%
TGAGAGAAATGAAACAATGA+TGG - chr2_3:4477818-4477837 Msa0272910:intron 30.0%
GAGAGAAATGAAACAATGAT+GGG - chr2_3:4477819-4477838 Msa0272910:intron 30.0%
! TTAAACATGTTGCCATTTGT+TGG - chr2_3:4478090-4478109 Msa0272910:intron 30.0%
TTAGCTTATTCCTCTTTGAT+AGG - chr2_3:4478124-4478143 Msa0272910:intron 30.0%
GTCATGTTATATAGTGTTGT+AGG - chr2_3:4478190-4478209 Msa0272910:intron 30.0%
TGCATTTCTTCATTGTGTAT+TGG - chr2_3:4478266-4478285 Msa0272910:intron 30.0%
CTTGTAGAATCTCATCAAAA+TGG + chr2_3:4478378-4478397 None:intergenic 30.0%
!! ATTCTGTTTTGTTGTGCTTT+AGG - chr2_3:4478512-4478531 Msa0272910:intron 30.0%
TGATATGTGCCAATAAAGTA+TGG + chr2_3:4478781-4478800 None:intergenic 30.0%
! GTACACCTAATTTTTATTCC+TGG - chr2_3:4479204-4479223 Msa0272910:intron 30.0%
CAATCCCAGGAATAAAAATT+AGG + chr2_3:4479212-4479231 None:intergenic 30.0%
CACCCATATCTTATATTGAA+AGG + chr2_3:4479426-4479445 None:intergenic 30.0%
CTTTCAATATAAGATATGGG+TGG - chr2_3:4479424-4479443 Msa0272910:intron 30.0%
TTTAAGAACCTAGACAGAAA+TGG + chr2_3:4479452-4479471 None:intergenic 30.0%
ACTAACTTCAGAAAGAGAAA+AGG + chr2_3:4479539-4479558 None:intergenic 30.0%
TCATTATTGAATGTACTGCA+GGG + chr2_3:4479622-4479641 None:intergenic 30.0%
TGCAGTACATTCAATAATGA+CGG - chr2_3:4479622-4479641 Msa0272910:intron 30.0%
ATTAGATATTTGAGCCTTGA+GGG - chr2_3:4479866-4479885 Msa0272910:intron 30.0%
! GTTGTTTCAGAGTCAAATTA+TGG + chr2_3:4479997-4480016 None:intergenic 30.0%
! AATGATAGTAACTTGCATAG+TGG - chr2_3:4480120-4480139 Msa0272910:CDS 30.0%
! ATAGTGGAGAAAATGAAACA+GGG - chr2_3:4480136-4480155 Msa0272910:CDS 30.0%
TCTTTCAATCACTACCAATT+TGG + chr2_3:4480326-4480345 None:intergenic 30.0%
GTTGTCCGATATCATATTAA+GGG + chr2_3:4480394-4480413 None:intergenic 30.0%
AGTTGTCCGATATCATATTA+AGG + chr2_3:4480395-4480414 None:intergenic 30.0%
TCGATTGATTGCTAAATATC+CGG - chr2_3:4480703-4480722 Msa0272910:intron 30.0%
TAAATATCCGGTTGTATTGT+AGG - chr2_3:4480715-4480734 Msa0272910:intron 30.0%
AAATATCCGGTTGTATTGTA+GGG - chr2_3:4480716-4480735 Msa0272910:intron 30.0%
AGAAATAAGAGAAAGCTCTT+GGG + chr2_3:4480879-4480898 None:intergenic 30.0%
GTGAGAAAATTTGACAAAAG+AGG + chr2_3:4480922-4480941 None:intergenic 30.0%
TTCTATTGCAGTTTCTAAAC+CGG - chr2_3:4481064-4481083 Msa0272910:intron 30.0%
AATAACAACCTTACGAATGT+TGG - chr2_3:4481125-4481144 Msa0272910:intron 30.0%
ATATGCCAATCTCTGATGAA+AGG - chr2_3:4477393-4477412 Msa0272910:three_prime_UTR 35.0%
TTGAACCTTTCATCAGAGAT+TGG + chr2_3:4477401-4477420 None:intergenic 35.0%
! AATTAGATAGAGTGCTTTGG+TGG - chr2_3:4477419-4477438 Msa0272910:three_prime_UTR 35.0%
! ATTAGATAGAGTGCTTTGGT+GGG - chr2_3:4477420-4477439 Msa0272910:three_prime_UTR 35.0%
TTCTTGCAGAATGTTCATCA+AGG - chr2_3:4477460-4477479 Msa0272910:three_prime_UTR 35.0%
GGAATTGTTGTCTATGAACT+TGG - chr2_3:4477662-4477681 Msa0272910:three_prime_UTR 35.0%
TGTGATTGCAGATGCTAAAA+AGG - chr2_3:4477782-4477801 Msa0272910:CDS 35.0%
GGATGAACTAATGAATGAAG+AGG - chr2_3:4477840-4477859 Msa0272910:intron 35.0%
TGAACTAATGAATGAAGAGG+AGG - chr2_3:4477843-4477862 Msa0272910:intron 35.0%
! GTCCTTTTTCTTCATTTTGC+TGG - chr2_3:4477954-4477973 Msa0272910:intron 35.0%
GACCAGCAAAATGAAGAAAA+AGG + chr2_3:4477959-4477978 None:intergenic 35.0%
! TTTTCTTCATTTTGCTGGTC+TGG - chr2_3:4477959-4477978 Msa0272910:intron 35.0%
! AATGTTGCTTCACCAACAAA+TGG + chr2_3:4478105-4478124 None:intergenic 35.0%
TTATTCCTCTTTGATAGGAG+TGG - chr2_3:4478129-4478148 Msa0272910:intron 35.0%
TTGAATCAGCATAGCTGATA+AGG + chr2_3:4478320-4478339 None:intergenic 35.0%
ACAAAGAATCTAACCTTCTC+TGG + chr2_3:4478451-4478470 None:intergenic 35.0%
TCATGGTTAGCATTCAATGA+AGG - chr2_3:4478898-4478917 Msa0272910:intron 35.0%
ACAAAACTTTATCGGATCCT+AGG + chr2_3:4479303-4479322 None:intergenic 35.0%
GTCATTATTGAATGTACTGC+AGG + chr2_3:4479623-4479642 None:intergenic 35.0%
AAACCCTTCATACTTATCGA+TGG - chr2_3:4479770-4479789 Msa0272910:intron 35.0%
GCTCAAATATCTAATCCAAG+TGG + chr2_3:4479861-4479880 None:intergenic 35.0%
GATTAGATATTTGAGCCTTG+AGG - chr2_3:4479865-4479884 Msa0272910:intron 35.0%
AATTGACAGTTTAACCCTCA+AGG + chr2_3:4479883-4479902 None:intergenic 35.0%
! AATTGAAGGCACATATCAAC+AGG + chr2_3:4479968-4479987 None:intergenic 35.0%
! AACTTTTACACATCGTCTAC+AGG - chr2_3:4480014-4480033 Msa0272910:intron 35.0%
!! GTCTACAGGTTTGTTATGAT+TGG - chr2_3:4480028-4480047 Msa0272910:intron 35.0%
! CATAGTGGAGAAAATGAAAC+AGG - chr2_3:4480135-4480154 Msa0272910:CDS 35.0%
GCATACTGCATAGTTTGATT+TGG - chr2_3:4480267-4480286 Msa0272910:intron 35.0%
TTCAATCACTACCAATTTGG+AGG + chr2_3:4480323-4480342 None:intergenic 35.0%
! TGGTAGTGATTGAAAGACTA+CGG - chr2_3:4480329-4480348 Msa0272910:intron 35.0%
TTAAGGGAAATTACTACTGC+AGG + chr2_3:4480378-4480397 None:intergenic 35.0%
AAGAAAAGGATGACACGAAA+AGG + chr2_3:4480501-4480520 None:intergenic 35.0%
! CATCCTTTTCTTTACCTTAC+TGG - chr2_3:4480509-4480528 Msa0272910:intron 35.0%
TTTAGAAAGAAAGGCCAGTA+AGG + chr2_3:4480526-4480545 None:intergenic 35.0%
AATATCCGGTTGTATTGTAG+GGG - chr2_3:4480717-4480736 Msa0272910:intron 35.0%
TGCTAAAGATACCAGAAATC+TGG - chr2_3:4480759-4480778 Msa0272910:intron 35.0%
! GTTCTTTTGTCTTCAGAACT+GGG + chr2_3:4480802-4480821 None:intergenic 35.0%
CAGAAATAAGAGAAAGCTCT+TGG + chr2_3:4480880-4480899 None:intergenic 35.0%
GAGTAGGATGATTGTTAAAG+AGG - chr2_3:4480987-4481006 Msa0272910:intron 35.0%
TCTACTTACCAACATTCGTA+AGG + chr2_3:4481136-4481155 None:intergenic 35.0%
TGCTTTCAGAAACCGAATCA+AGG + chr2_3:4477500-4477519 None:intergenic 40.0%
!! TGATTCGGTTTCTGAAAGCA+AGG - chr2_3:4477500-4477519 Msa0272910:three_prime_UTR 40.0%
! GATTCGGTTTCTGAAAGCAA+GGG - chr2_3:4477501-4477520 Msa0272910:three_prime_UTR 40.0%
AAGGGAATGGAATGCTTCAA+AGG - chr2_3:4477519-4477538 Msa0272910:three_prime_UTR 40.0%
GCAGATGCTAAAAAGGATGA+AGG - chr2_3:4477789-4477808 Msa0272910:CDS 40.0%
AGTAGAAGAACAGCAAGAAG+AGG - chr2_3:4477870-4477889 Msa0272910:intron 40.0%
!!! CATTTTGCTGGTCTGGTTTT+TGG - chr2_3:4477966-4477985 Msa0272910:intron 40.0%
!! GTGGTTACTAGCTTTCTTTG+TGG - chr2_3:4477990-4478009 Msa0272910:intron 40.0%
!!! GTTTTTTTCCTGCTGTGTCT+AGG - chr2_3:4478048-4478067 Msa0272910:intron 40.0%
GAGATTCTACAAGAAGCTGA+TGG - chr2_3:4478385-4478404 Msa0272910:CDS 40.0%
AAGAATCTAACCTTCTCTGG+TGG + chr2_3:4478448-4478467 None:intergenic 40.0%
GGACATTTGGTTACTTCCAA+AGG + chr2_3:4478831-4478850 None:intergenic 40.0%
TGAGCTCTTGTATTCTTCAC+CGG + chr2_3:4479054-4479073 None:intergenic 40.0%
TCCTGGGATTGTAGTTCAAT+CGG - chr2_3:4479221-4479240 Msa0272910:intron 40.0%
AACTTTATCGGATCCTAGGT+CGG + chr2_3:4479299-4479318 None:intergenic 40.0%
CAGTGAAAAATACGGGCAAA+AGG + chr2_3:4479337-4479356 None:intergenic 40.0%
! TTTGCCCGTATTTTTCACTG+TGG - chr2_3:4479337-4479356 Msa0272910:intron 40.0%
! TTGCCCGTATTTTTCACTGT+GGG - chr2_3:4479338-4479357 Msa0272910:intron 40.0%
GTTAGAGACAAGTTTCCTCA+CGG + chr2_3:4479365-4479384 None:intergenic 40.0%
TTGAATGTACTGCAGGGTAT+GGG + chr2_3:4479616-4479635 None:intergenic 40.0%
ATTGAATGTACTGCAGGGTA+TGG + chr2_3:4479617-4479636 None:intergenic 40.0%
GGTCCATCGATAAGTATGAA+GGG + chr2_3:4479776-4479795 None:intergenic 40.0%
AGGTCCATCGATAAGTATGA+AGG + chr2_3:4479777-4479796 None:intergenic 40.0%
! CATGATGTTTCTGCGAAATG+AGG + chr2_3:4479797-4479816 None:intergenic 40.0%
TATCTAATCCAAGTGGCAGA+TGG + chr2_3:4479854-4479873 None:intergenic 40.0%
AGTAATCCTCAAGAGCTTCA+TGG - chr2_3:4480165-4480184 Msa0272910:CDS 40.0%
GTAATCCTCAAGAGCTTCAT+GGG - chr2_3:4480166-4480185 Msa0272910:CDS 40.0%
CAAACTATGCAGTATGCTTG+AGG + chr2_3:4480264-4480283 None:intergenic 40.0%
!! TACTGCAGGCGATATTTTTG+GGG + chr2_3:4480364-4480383 None:intergenic 40.0%
! CTACTGCAGGCGATATTTTT+GGG + chr2_3:4480365-4480384 None:intergenic 40.0%
! ACTACTGCAGGCGATATTTT+TGG + chr2_3:4480366-4480385 None:intergenic 40.0%
! ACGAAAAGGATCAGTGGATT+TGG + chr2_3:4480487-4480506 None:intergenic 40.0%
AGGCCAGTAAGGTAAAGAAA+AGG + chr2_3:4480515-4480534 None:intergenic 40.0%
! CGTTCTTTTGTCTTCAGAAC+TGG + chr2_3:4480803-4480822 None:intergenic 40.0%
AGAAGATGCTTGACCTGATA+AGG + chr2_3:4481093-4481112 None:intergenic 40.0%
! GGTTTCTGAAAGCAAGGGAA+TGG - chr2_3:4477506-4477525 Msa0272910:three_prime_UTR 45.0%
ACACCAAAACTCAGCAACAC+AGG + chr2_3:4477936-4477955 None:intergenic 45.0%
TAGGAGTGGTGTGGTATCAT+AGG - chr2_3:4478143-4478162 Msa0272910:intron 45.0%
ATGGAATCATCTTGTCCCGT+GGG - chr2_3:4478404-4478423 Msa0272910:CDS 45.0%
ATCTTGTCCCGTGGGAATTT+GGG - chr2_3:4478412-4478431 Msa0272910:CDS 45.0%
ATCAATGCCCAAATTCCCAC+GGG + chr2_3:4478422-4478441 None:intergenic 45.0%
GATCAATGCCCAAATTCCCA+CGG + chr2_3:4478423-4478442 None:intergenic 45.0%
CATTGATCTTCCACCAGAGA+AGG - chr2_3:4478435-4478454 Msa0272910:CDS 45.0%
TGCACTGTGACTGTTAATCC+CGG - chr2_3:4479032-4479051 Msa0272910:intron 45.0%
GAGCTCTTGTATTCTTCACC+GGG + chr2_3:4479053-4479072 None:intergenic 45.0%
ACCGATTGAACTACAATCCC+AGG + chr2_3:4479225-4479244 None:intergenic 45.0%
ACTTTATCGGATCCTAGGTC+GGG + chr2_3:4479298-4479317 None:intergenic 45.0%
! TGCCCGTATTTTTCACTGTG+GGG - chr2_3:4479339-4479358 Msa0272910:intron 45.0%
CGGTTCCATATATGCTTACG+CGG - chr2_3:4479642-4479661 Msa0272910:intron 45.0%
GCATACCGCGTAAGCATATA+TGG + chr2_3:4479650-4479669 None:intergenic 45.0%
TGGAGCAACTACTGCAAAGA+TGG - chr2_3:4480048-4480067 Msa0272910:intron 45.0%
AGCAACTACTGCAAAGATGG+TGG - chr2_3:4480051-4480070 Msa0272910:intron 45.0%
!! ACTGCAAAGATGGTGGATCT+TGG - chr2_3:4480058-4480077 Msa0272910:intron 45.0%
! ACCAGCTTTGCAGTGGATTT+AGG + chr2_3:4480099-4480118 None:intergenic 45.0%
ATCATTGACCAGCTTTGCAG+TGG + chr2_3:4480106-4480125 None:intergenic 45.0%
AACTTCCCATGAAGCTCTTG+AGG + chr2_3:4480174-4480193 None:intergenic 45.0%
TACACGATCAACAGGACCAA+CGG + chr2_3:4480223-4480242 None:intergenic 45.0%
GGAGTAGATACACGATCAAC+AGG + chr2_3:4480231-4480250 None:intergenic 45.0%
GCAGTATGCTTGAGGATTAC+CGG + chr2_3:4480256-4480275 None:intergenic 45.0%
GATGACACGAAAAGGATCAG+TGG + chr2_3:4480493-4480512 None:intergenic 45.0%
!! TATTAGAGTTGGTACGGGCA+AGG + chr2_3:4480584-4480603 None:intergenic 45.0%
ATGTGCCCCTACAATACAAC+CGG + chr2_3:4480725-4480744 None:intergenic 45.0%
! TGTATTGTAGGGGCACATCA+AGG - chr2_3:4480727-4480746 Msa0272910:intron 45.0%
TGCTTGAACAGCCAGATTTC+TGG + chr2_3:4480773-4480792 None:intergenic 45.0%
ATTATGTGGGGAGCAAGAGT+AGG - chr2_3:4480971-4480990 Msa0272910:intron 45.0%
TCTAAACCGGTCACCTTATC+AGG - chr2_3:4481077-4481096 Msa0272910:intron 45.0%
!!! AAAAAAAATATCTTTAAAAT+AGG + chr2_3:4478650-4478669 None:intergenic 5.0%
!! CAGCCTGTGTTGCTGAGTTT+TGG - chr2_3:4477930-4477949 Msa0272910:intron 50.0%
!!! TGCTGGTCTGGTTTTTGGTG+TGG - chr2_3:4477971-4477990 Msa0272910:intron 50.0%
CCACACCACTCCTATCAAAG+AGG + chr2_3:4478137-4478156 None:intergenic 50.0%
CCTCTTTGATAGGAGTGGTG+TGG - chr2_3:4478134-4478153 Msa0272910:intron 50.0%
GATGGAATCATCTTGTCCCG+TGG - chr2_3:4478403-4478422 Msa0272910:CDS 50.0%
CATCTTGTCCCGTGGGAATT+TGG - chr2_3:4478411-4478430 Msa0272910:CDS 50.0%
GGCCCCACAGTGAAAAATAC+GGG + chr2_3:4479344-4479363 None:intergenic 50.0%
CGGCCCCACAGTGAAAAATA+CGG + chr2_3:4479345-4479364 None:intergenic 50.0%
GGGTGGTTCCATTTCTGTCT+AGG - chr2_3:4479441-4479460 Msa0272910:intron 50.0%
GCTTAGATCCATCTGCCACT+TGG - chr2_3:4479843-4479862 Msa0272910:intron 50.0%
GCCTAAATCCACTGCAAAGC+TGG - chr2_3:4480095-4480114 Msa0272910:intron 50.0%
CATGGGAAGTTGTCTGCTAC+TGG - chr2_3:4480183-4480202 Msa0272910:intron 50.0%
GATCGTGTATCTACTCCAGC+CGG - chr2_3:4480234-4480253 Msa0272910:intron 50.0%
TATGCTTGAGGATTACCGGC+TGG + chr2_3:4480252-4480271 None:intergenic 50.0%
TTGGTACGGGCAAGGTCTAA+AGG + chr2_3:4480576-4480595 None:intergenic 50.0%
GCTTGACCTGATAAGGTGAC+CGG + chr2_3:4481086-4481105 None:intergenic 50.0%
TTCCTGCTGTGTCTAGGTGC+AGG - chr2_3:4478054-4478073 Msa0272910:intron 55.0%
TCCTGCTGTGTCTAGGTGCA+GGG - chr2_3:4478055-4478074 Msa0272910:intron 55.0%
TGTGTCTAGGTGCAGGGTCT+GGG - chr2_3:4478061-4478080 Msa0272910:intron 55.0%
! TTTTTCACTGTGGGGCCGTG+AGG - chr2_3:4479347-4479366 Msa0272910:intron 55.0%
GATCAACAGGACCAACGGCA+CGG + chr2_3:4480218-4480237 None:intergenic 55.0%
ACCCTGCACCTAGACACAGC+AGG + chr2_3:4478059-4478078 None:intergenic 60.0%
CTGTGTCTAGGTGCAGGGTC+TGG - chr2_3:4478060-4478079 Msa0272910:intron 60.0%
ATCCTAGGTCGGGACTGACG+GGG + chr2_3:4479288-4479307 None:intergenic 60.0%
GATCCTAGGTCGGGACTGAC+GGG + chr2_3:4479289-4479308 None:intergenic 60.0%
GGATCCTAGGTCGGGACTGA+CGG + chr2_3:4479290-4479309 None:intergenic 60.0%
!! GTCAGAGCACCGCCCTGTCA+TGG - chr2_3:4479243-4479262 Msa0272910:intron 65.0%
!! AGAGCACCGCCCTGTCATGG+CGG - chr2_3:4479246-4479265 Msa0272910:intron 65.0%
CAGCTTCCGCCATGACAGGG+CGG + chr2_3:4479255-4479274 None:intergenic 65.0%
CCGCAGCTTCCGCCATGACA+GGG + chr2_3:4479258-4479277 None:intergenic 65.0%
! CCCTGTCATGGCGGAAGCTG+CGG - chr2_3:4479255-4479274 Msa0272910:intron 65.0%
! CCTGTCATGGCGGAAGCTGC+GGG - chr2_3:4479256-4479275 Msa0272910:intron 65.0%
! GGCAGTTCTAGCCGTGCCGT+TGG - chr2_3:4480204-4480223 Msa0272910:intron 65.0%
CCCGCAGCTTCCGCCATGAC+AGG + chr2_3:4479259-4479278 None:intergenic 70.0%
AGCCCCGTCAGTCCCGACCT+AGG - chr2_3:4479283-4479302 Msa0272910:intron 70.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 4477351 4481214 4477351 ID=Msa0272910;Name=Msa0272910
chr2_3 mRNA 4477351 4481214 4477351 ID=Msa0272910-mRNA-1;Parent=Msa0272910;Name=Msa0272910-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0|0.2|0.16|0.33|1|1|6|386|188
chr2_3 exon 4477351 4477828 4477351 ID=Msa0272910-mRNA-1:exon:2230;Parent=Msa0272910-mRNA-1
chr2_3 exon 4478310 4478529 4478310 ID=Msa0272910-mRNA-1:exon:2229;Parent=Msa0272910-mRNA-1
chr2_3 exon 4478902 4478973 4478902 ID=Msa0272910-mRNA-1:exon:2228;Parent=Msa0272910-mRNA-1
chr2_3 exon 4480109 4480198 4480109 ID=Msa0272910-mRNA-1:exon:2227;Parent=Msa0272910-mRNA-1
chr2_3 exon 4480410 4480495 4480410 ID=Msa0272910-mRNA-1:exon:2226;Parent=Msa0272910-mRNA-1
chr2_3 exon 4481208 4481214 4481208 ID=Msa0272910-mRNA-1:exon:2225;Parent=Msa0272910-mRNA-1
chr2_3 CDS 4481208 4481214 4481208 ID=Msa0272910-mRNA-1:cds;Parent=Msa0272910-mRNA-1
chr2_3 CDS 4480410 4480495 4480410 ID=Msa0272910-mRNA-1:cds;Parent=Msa0272910-mRNA-1
chr2_3 CDS 4480109 4480198 4480109 ID=Msa0272910-mRNA-1:cds;Parent=Msa0272910-mRNA-1
chr2_3 CDS 4478902 4478973 4478902 ID=Msa0272910-mRNA-1:cds;Parent=Msa0272910-mRNA-1
chr2_3 CDS 4478310 4478529 4478310 ID=Msa0272910-mRNA-1:cds;Parent=Msa0272910-mRNA-1
chr2_3 CDS 4477737 4477828 4477737 ID=Msa0272910-mRNA-1:cds;Parent=Msa0272910-mRNA-1
chr2_3 three_prime_UTR 4477351 4477736 4477351 ID=Msa0272910-mRNA-1:three_prime_utr;Parent=Msa0272910-mRNA-1
Gene Sequence

>Msa0272910

ATGGCAGGTGCAGGGTCTGGGTTAATATTAAACATGTTGCCATTTGTTGGTGAAGCAACATTTAGCTTATTCCTCTTTGATAGGAGTGGTGTGGGATTGACCCATTTTGATGAGATTCTACAAGAAGCTGATGGAATCATCTTGTCCCGTGGGAATTTGGGCATTGATCTTCCACCAGAGAAGAATATGCAGAGGTGTAGTCAGCCCATACCCTGCAGTACATTCAATAATGACGGTTCCATATATGCTTACGCGGTTTGTTATGATTGGAGCAACTACTGCAAAGATGGTGGATCTTGGTCGACAACATTCTCGCCTAAATCCACTGCAAAGCTGGTCAATGATAGTAACTTGCATAGTGGAGAAAATGAAACAGGGTTAGTGAGTAATCCTCAAGAGCTTCATGGGAAGTTGTCTGCTACTGGCAGTTCTAGCCGTGCCGTTGGTCCTGTTGATCGTGTATCTACTCCAGCCGGGGCACATCAAGGAAGCATTTTTGCTAAAGATACCAGAAATCTGGCTGTTCAAGCAGCAGATCCCAGTTCTGAAGACAAAAGAACGCAGTGA
Protein sequence

>Msa0272910

MAGAGSGLILNMLPFVGEATFSLFLFDRSGVGLTHFDEILQEADGIILSRGNLGIDLPPEKNMQRCSQPIPCSTFNNDGSIYAYAVCYDWSNYCKDGGSWSTTFSPKSTAKLVNDSNLHSGENETGLVSNPQELHGKLSATGSSSRAVGPVDRVSTPAGAHQGSIFAKDTRNLAVQAADPSSEDKRTQ*