AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1012260

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1012260 MtrunA17_Chr3g0138401 88.542 96 8 1 96 188 321 416 1.16e-50 168
Msa1012260 MtrunA17_Chr1g0198231 96.774 31 1 0 61 91 291 321 2.14e-15 73.2
Msa1012260 MtrunA17_Chr8g0367631 93.548 31 2 0 61 91 281 311 1.24e-14 70.9
Msa1012260 MtrunA17_Chr7g0265591 100.000 30 0 0 32 61 262 291 1.61e-13 68.2
Msa1012260 MtrunA17_Chr1g0179541 100.000 30 0 0 32 61 246 275 2.14e-13 67.8
Msa1012260 MtrunA17_Chr2g0278901 80.645 31 6 0 61 91 284 314 2.59e-12 64.3
Msa1012260 MtrunA17_Chr1g0197911 90.000 30 3 0 32 61 261 290 1.13e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1012260 AT3G52990.2 100.000 30 0 0 32 61 209 238 2.54e-13 67.8
Msa1012260 AT3G52990.1 100.000 30 0 0 32 61 262 291 2.63e-13 67.8
Msa1012260 AT2G36580.1 100.000 30 0 0 32 61 262 291 2.98e-13 67.8
Msa1012260 AT1G80670.1 80.645 31 6 0 61 91 282 312 4.07e-12 63.9

Find 63 sgRNAs with CRISPR-Local

Find 254 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCTTGTCCCGTGGGAATT+TGG 0.131046 7_1:-17368615 Msa1012260:CDS
TGCTTGAACAGCCAGATTTC+TGG 0.202017 7_1:+17365933 None:intergenic
TCTAAACCGGTCACCTTATC+AGG 0.216944 7_1:-17365626 Msa1012260:three_prime_UTR
CTGTGTCTAGGTGCAGGGTC+TGG 0.269441 7_1:-17368966 Msa1012260:intron
CTTGTAGAATCTCATCAAAA+TGG 0.282372 7_1:+17368651 None:intergenic
ACCAGCTTTGCAGTGGATTT+AGG 0.291769 7_1:+17366624 None:intergenic
ATCTTGTCCCGTGGGAATTT+GGG 0.295886 7_1:-17368614 Msa1012260:CDS
GCATACCGCGTAAGCATATA+TGG 0.299683 7_1:+17367073 None:intergenic
TGCTAAAGATACCAGAAATC+TGG 0.323635 7_1:-17365944 Msa1012260:CDS
GTCTACAGGTTTGTTATGAT+TGG 0.341574 7_1:-17366692 Msa1012260:intron
TTAGCTTATTCCTCTTTGAT+AGG 0.342074 7_1:-17368902 Msa1012260:CDS
CATAGTGGAGAAAATGAAAC+AGG 0.343306 7_1:-17366585 Msa1012260:CDS
CAGAAATAAGAGAAAGCTCT+TGG 0.383667 7_1:+17365826 None:intergenic
TTCTATTGCAGTTTCTAAAC+CGG 0.384539 7_1:-17365639 Msa1012260:three_prime_UTR
TGTGTCTAGGTGCAGGGTCT+GGG 0.392512 7_1:-17368965 Msa1012260:intron
CATGGGAAGTTGTCTGCTAC+TGG 0.416162 7_1:-17366537 Msa1012260:CDS
ATTGAATGTACTGCAGGGTA+TGG 0.417856 7_1:+17367106 None:intergenic
ACAAAGAATCTAACCTTCTC+TGG 0.426853 7_1:+17368578 None:intergenic
GCCTAAATCCACTGCAAAGC+TGG 0.430607 7_1:-17366625 Msa1012260:CDS
TCCTGCTGTGTCTAGGTGCA+GGG 0.434688 7_1:-17368971 Msa1012260:intron
AGAAGATGCTTGACCTGATA+AGG 0.443032 7_1:+17365613 None:intergenic
GTCATTATTGAATGTACTGC+AGG 0.456046 7_1:+17367100 None:intergenic
TTAAACATGTTGCCATTTGT+TGG 0.456841 7_1:-17368936 Msa1012260:CDS
TTGTAGAATCTCATCAAAAT+GGG 0.469141 7_1:+17368652 None:intergenic
GGAGTAGATACACGATCAAC+AGG 0.476638 7_1:+17366492 None:intergenic
ACTGCAAAGATGGTGGATCT+TGG 0.479375 7_1:-17366662 Msa1012260:CDS
GTGAGAAAATTTGACAAAAG+AGG 0.485887 7_1:+17365784 None:intergenic
AGAAATAAGAGAAAGCTCTT+GGG 0.490918 7_1:+17365827 None:intergenic
AGTAATCCTCAAGAGCTTCA+TGG 0.497229 7_1:-17366555 Msa1012260:CDS
TTATTCCTCTTTGATAGGAG+TGG 0.500496 7_1:-17368897 Msa1012260:CDS
ATGTTTCTGTAATCAATGGC+AGG 0.504577 7_1:-17369694 None:intergenic
GAGTAGGATGATTGTTAAAG+AGG 0.509074 7_1:-17365716 Msa1012260:three_prime_UTR
AACTTCCCATGAAGCTCTTG+AGG 0.518605 7_1:+17366549 None:intergenic
ATTATGTGGGGAGCAAGAGT+AGG 0.524042 7_1:-17365732 Msa1012260:three_prime_UTR
GTAATCCTCAAGAGCTTCAT+GGG 0.525373 7_1:-17366554 Msa1012260:CDS
AATAACAACCTTACGAATGT+TGG 0.526846 7_1:-17365578 Msa1012260:three_prime_UTR
AATGTTGCTTCACCAACAAA+TGG 0.529121 7_1:+17368924 None:intergenic
CATTGATCTTCCACCAGAGA+AGG 0.538672 7_1:-17368591 Msa1012260:intron
ATCATTGACCAGCTTTGCAG+TGG 0.539773 7_1:+17366617 None:intergenic
GGCAGTTCTAGCCGTGCCGT+TGG 0.541164 7_1:-17366516 Msa1012260:CDS
TGGAGCAACTACTGCAAAGA+TGG 0.553115 7_1:-17366672 Msa1012260:CDS
TCTACTTACCAACATTCGTA+AGG 0.553647 7_1:+17365570 None:intergenic
GCTTGACCTGATAAGGTGAC+CGG 0.558454 7_1:+17365620 None:intergenic
AATGATAGTAACTTGCATAG+TGG 0.579416 7_1:-17366600 Msa1012260:CDS
GAGATTCTACAAGAAGCTGA+TGG 0.579505 7_1:-17368641 Msa1012260:CDS
CCTCTTTGATAGGAGTGGTG+TGG 0.581690 7_1:-17368892 Msa1012260:intron
GATCGTGTATCTACTCCAGC+CGG 0.597972 7_1:-17366486 Msa1012260:intron
TACACGATCAACAGGTCCAA+CGG 0.603243 7_1:+17366500 None:intergenic
ATGGAATCATCTTGTCCCGT+GGG 0.603759 7_1:-17368622 Msa1012260:CDS
TAATTGATGTATCTGTTAGA+CGG 0.604298 7_1:+17365692 None:intergenic
TGCAGTACATTCAATAATGA+CGG 0.604536 7_1:-17367098 Msa1012260:CDS
GATCAACAGGTCCAACGGCA+CGG 0.605689 7_1:+17366505 None:intergenic
CCACACCACTCCTATCAAAG+AGG 0.605958 7_1:+17368892 None:intergenic
AAGAATCTAACCTTCTCTGG+TGG 0.606089 7_1:+17368581 None:intergenic
ATCAATGCCCAAATTCCCAC+GGG 0.610460 7_1:+17368607 None:intergenic
TGTATTGTAGGGGCACATCA+AGG 0.610537 7_1:-17365976 Msa1012260:intron
TTGAATGTACTGCAGGGTAT+GGG 0.618328 7_1:+17367107 None:intergenic
AGCAACTACTGCAAAGATGG+TGG 0.619072 7_1:-17366669 Msa1012260:CDS
GATCAATGCCCAAATTCCCA+CGG 0.634784 7_1:+17368606 None:intergenic
GATGGAATCATCTTGTCCCG+TGG 0.645597 7_1:-17368623 Msa1012260:CDS
TCATTATTGAATGTACTGCA+GGG 0.646171 7_1:+17367101 None:intergenic
CGGTTCCATATATGCTTACG+CGG 0.673675 7_1:-17367078 Msa1012260:intron
ATAGTGGAGAAAATGAAACA+GGG 0.722474 7_1:-17366584 Msa1012260:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAGAAAAATTTGATTTTTT+TGG - chr7_1:17365884-17365903 Msa1012260:exon 10.0%
!!! CATAATCTTTTTTTTTTTTA+AGG + chr7_1:17369444-17369463 None:intergenic 10.0%
!! TAAAAAAAAAAAAGATTATG+TGG - chr7_1:17369444-17369463 Msa1012260:intron 10.0%
!! AAAAAAAAAAAAGATTATGT+GGG - chr7_1:17369445-17369464 Msa1012260:intron 10.0%
!!! AAATTTGATTTTTTTGGATT+TGG - chr7_1:17365890-17365909 Msa1012260:exon 15.0%
!!! TTTATATATCTGTTCTTTTT+AGG - chr7_1:17366509-17366528 Msa1012260:CDS 15.0%
!!! TTATATATCTGTTCTTTTTA+GGG - chr7_1:17366510-17366529 Msa1012260:CDS 15.0%
!! GTACTTAATAATTCTTAAAA+TGG - chr7_1:17366713-17366732 Msa1012260:intron 15.0%
!!! ATATTTTTGTTTGTTTTATG+AGG - chr7_1:17366745-17366764 Msa1012260:intron 15.0%
!!! TATCTAATCTAAGAATTTTT+AGG - chr7_1:17367649-17367668 Msa1012260:intron 15.0%
!! TTCTAAATTCATTAGAATAT+TGG - chr7_1:17369026-17369045 Msa1012260:intron 15.0%
!!! TTTTTATGTTCATATTCAAT+GGG - chr7_1:17369337-17369356 Msa1012260:intron 15.0%
!! AAAAAAAAAAAGATTATGTG+GGG - chr7_1:17369446-17369465 Msa1012260:intron 15.0%
!!! AGAATGAATATAACAATTTT+TGG - chr7_1:17369659-17369678 Msa1012260:intron 15.0%
!! TAGGAAAGATTGAATAAAAT+TGG + chr7_1:17366795-17366814 None:intergenic 20.0%
!!! AGATTTTTACTCTCTAAAAT+AGG + chr7_1:17366814-17366833 None:intergenic 20.0%
!!! TTAATCTCAGATTTTTTCTT+TGG - chr7_1:17366873-17366892 Msa1012260:intron 20.0%
!! AGATAAGGAAAATAAATCTT+AGG + chr7_1:17367114-17367133 None:intergenic 20.0%
!! AAGGCAAATAAATAAATCTT+AGG + chr7_1:17367282-17367301 None:intergenic 20.0%
!! AAGATTTATTTATTTGCCTT+TGG - chr7_1:17367282-17367301 Msa1012260:intron 20.0%
!! AAACCTTAACATAGTAATTA+TGG + chr7_1:17367322-17367341 None:intergenic 20.0%
!!! TTACTATGTTAAGGTTTTAT+TGG - chr7_1:17367325-17367344 Msa1012260:intron 20.0%
!!! TTTTATATACATGATCATCA+TGG - chr7_1:17367351-17367370 Msa1012260:intron 20.0%
!! ATTTCTCTTCATTATTCATT+AGG + chr7_1:17367616-17367635 None:intergenic 20.0%
!! AATAACAGACAAAACTTTAT+CGG + chr7_1:17367781-17367800 None:intergenic 20.0%
!! ATCCTTTCAATATAAGATAT+GGG - chr7_1:17367891-17367910 Msa1012260:intron 20.0%
!! TTAATAGTGTACAGTAATTA+CGG + chr7_1:17367946-17367965 None:intergenic 20.0%
!!! TATTTTCTAGATTTGATACA+TGG + chr7_1:17367969-17367988 None:intergenic 20.0%
!! ACATAGAAAGTAAATAACTA+CGG + chr7_1:17368164-17368183 None:intergenic 20.0%
!!! ATAATTTGCTTGCTATTTTA+TGG - chr7_1:17368411-17368430 Msa1012260:intron 20.0%
!! AATTTCCCTTAATATGATAT+CGG - chr7_1:17368856-17368875 Msa1012260:intron 20.0%
!! TCTAATGAATTTAGAAAGAA+AGG + chr7_1:17369022-17369041 None:intergenic 20.0%
!! AATCAATAAAATATTCGAGT+TGG + chr7_1:17369082-17369101 None:intergenic 20.0%
!!! GTTTTTATGTTCATATTCAA+TGG - chr7_1:17369336-17369355 Msa1012260:intron 20.0%
!!! ACAATTTTTGGTTTAAAAAG+AGG - chr7_1:17369671-17369690 Msa1012260:intron 20.0%
!! ATGACTCAAATTTTCTGATA+TGG - chr7_1:17365521-17365540 Msa1012260:three_prime_UTR 25.0%
!!! TCTTCTTTTAGGCTTTAATT+TGG - chr7_1:17365750-17365769 Msa1012260:three_prime_UTR 25.0%
!! ATTTAGTGTTATGCTCTAAA+TGG - chr7_1:17365800-17365819 Msa1012260:three_prime_UTR 25.0%
!! TTTAGTGTTATGCTCTAAAT+GGG - chr7_1:17365801-17365820 Msa1012260:three_prime_UTR 25.0%
! AAAAACAGGAAAATAAATGC+AGG + chr7_1:17365919-17365938 None:intergenic 25.0%
!!! CTTTTTTTATCGTTTTTGCT+GGG - chr7_1:17366190-17366209 Msa1012260:intron 25.0%
! TCATCTTATATAGTGTTGTA+GGG - chr7_1:17366355-17366374 Msa1012260:intron 25.0%
! GACACTTATAATTCTACATT+AGG - chr7_1:17366377-17366396 Msa1012260:intron 25.0%
!! ATCTAAGATTGAAAAGATTG+AGG - chr7_1:17366402-17366421 Msa1012260:intron 25.0%
! TTGTAGAATCTCATCAAAAT+GGG + chr7_1:17366541-17366560 None:intergenic 25.0%
!!! ATTTTGTTTTGTTGTGCTTT+AGG - chr7_1:17366676-17366695 Msa1012260:CDS 25.0%
! ATAATTCTTAAAATGGCGTT+TGG - chr7_1:17366720-17366739 Msa1012260:intron 25.0%
! ATATTGAGACCATACTTTAT+TGG - chr7_1:17367239-17367258 Msa1012260:intron 25.0%
! ACATAGTAATTATGGACATT+TGG + chr7_1:17367314-17367333 None:intergenic 25.0%
! TGTCCATAATTACTATGTTA+AGG - chr7_1:17367316-17367335 Msa1012260:intron 25.0%
!! TCACTCTTTTACAGTATAAA+GGG + chr7_1:17367455-17367474 None:intergenic 25.0%
!! TTCACTCTTTTACAGTATAA+AGG + chr7_1:17367456-17367475 None:intergenic 25.0%
!! TACACCTAATTTTTATTCCT+GGG - chr7_1:17367675-17367694 Msa1012260:intron 25.0%
! TCACTTCATAGTAAAATTGA+AGG - chr7_1:17367865-17367884 Msa1012260:intron 25.0%
! GATCCTTTCAATATAAGATA+TGG - chr7_1:17367890-17367909 Msa1012260:intron 25.0%
! AATAACTTCAGAAAGAGAAA+AGG + chr7_1:17368009-17368028 None:intergenic 25.0%
!!! TTTGTTTCTAGCTTTATAGT+TGG - chr7_1:17368200-17368219 Msa1012260:intron 25.0%
! AATTATGGTAACTGAATTGA+AGG + chr7_1:17368452-17368471 None:intergenic 25.0%
! GTATTATAATACCTCCAAAT+TGG - chr7_1:17368779-17368798 Msa1012260:intron 25.0%
!! TGATTGTTCAAAGCTAATTT+TGG + chr7_1:17368902-17368921 None:intergenic 25.0%
!!! ATAAAATATTCGAGTTGGTA+CGG + chr7_1:17369077-17369096 None:intergenic 25.0%
!! AGAAGACAAGAATTTTCATA+TGG + chr7_1:17369121-17369140 None:intergenic 25.0%
! TGAAAATTCTTGTCTTCTTT+AGG - chr7_1:17369123-17369142 Msa1012260:intron 25.0%
! TAATTGATGTATCTGTTAGA+CGG + chr7_1:17369501-17369520 None:intergenic 25.0%
!!! GCAATAGAAGATGTTTTTTA+CGG + chr7_1:17369540-17369559 None:intergenic 25.0%
! TTCAATTAGATAGAGTGCTT+TGG - chr7_1:17365576-17365595 Msa1012260:three_prime_UTR 30.0%
ATGTAACTGAAACCTTGATT+CGG - chr7_1:17365645-17365664 Msa1012260:three_prime_UTR 30.0%
! ATGCTTCAAAGGCTTTAATT+TGG - chr7_1:17365690-17365709 Msa1012260:three_prime_UTR 30.0%
! TGCTTCAAAGGCTTTAATTT+GGG - chr7_1:17365691-17365710 Msa1012260:three_prime_UTR 30.0%
!! CAAGTTGAGTTTCTTCTTTT+AGG - chr7_1:17365739-17365758 Msa1012260:three_prime_UTR 30.0%
!! AGGCTTTAATTTGGTTGTAT+AGG - chr7_1:17365759-17365778 Msa1012260:three_prime_UTR 30.0%
GAATTGTTGTCTATGAACTT+GGG - chr7_1:17365823-17365842 Msa1012260:three_prime_UTR 30.0%
TCTGCAATCACATAAAAAAC+AGG + chr7_1:17365933-17365952 None:intergenic 30.0%
TGAGAGAAATGAAACAATGA+TGG - chr7_1:17365977-17365996 Msa1012260:intron 30.0%
GAGAGAAATGAAACAATGAT+GGG - chr7_1:17365978-17365997 Msa1012260:intron 30.0%
!!! GCTTTTTTTATCGTTTTTGC+TGG - chr7_1:17366189-17366208 Msa1012260:intron 30.0%
! TTAAACATGTTGCCATTTGT+TGG - chr7_1:17366254-17366273 Msa1012260:intron 30.0%
TTAGCTTATTCCTCTTTGAT+AGG - chr7_1:17366288-17366307 Msa1012260:intron 30.0%
GTCATCTTATATAGTGTTGT+AGG - chr7_1:17366354-17366373 Msa1012260:intron 30.0%
TGCATTTCTTCATTGTGTAT+TGG - chr7_1:17366430-17366449 Msa1012260:intron 30.0%
CTTGTAGAATCTCATCAAAA+TGG + chr7_1:17366542-17366561 None:intergenic 30.0%
TAACATTATGTGATGACTTG+AGG - chr7_1:17366969-17366988 Msa1012260:intron 30.0%
! ATATTGAGACCATACTCTTT+TGG - chr7_1:17367072-17367091 Msa1012260:intron 30.0%
TGATATGTACCAAAAGAGTA+TGG + chr7_1:17367084-17367103 None:intergenic 30.0%
TCATATGATGGTAACAGATA+AGG + chr7_1:17367129-17367148 None:intergenic 30.0%
AGTCTCAAGTTATCATATGA+TGG + chr7_1:17367141-17367160 None:intergenic 30.0%
GCAAACTAATATGATAAGTG+AGG + chr7_1:17367006-17367025 None:intergenic 30.0%
TGATATGTGCCAATAAAGTA+TGG + chr7_1:17367251-17367270 None:intergenic 30.0%
! GTACACCTAATTTTTATTCC+TGG - chr7_1:17367674-17367693 Msa1012260:intron 30.0%
CAATCCCAGGAATAAAAATT+AGG + chr7_1:17367682-17367701 None:intergenic 30.0%
CACCCATATCTTATATTGAA+AGG + chr7_1:17367896-17367915 None:intergenic 30.0%
CTTTCAATATAAGATATGGG+TGG - chr7_1:17367894-17367913 Msa1012260:intron 30.0%
TTTAAGAACCTAGACAGAAA+TGG + chr7_1:17367922-17367941 None:intergenic 30.0%
!!! ATTTTTTGCAGAATATGCAG+AGG - chr7_1:17368051-17368070 Msa1012260:intron 30.0%
TCATTATTGAATGTACTGCA+GGG + chr7_1:17368092-17368111 None:intergenic 30.0%
TGCAGTACATTCAATAATGA+CGG - chr7_1:17368092-17368111 Msa1012260:intron 30.0%
ATTAGATATTTGAGCCTTGA+GGG - chr7_1:17368336-17368355 Msa1012260:intron 30.0%
! GTTGTTTCAGAGTCAAATTA+TGG + chr7_1:17368467-17368486 None:intergenic 30.0%
! AATGATAGTAACTTGCATAG+TGG - chr7_1:17368590-17368609 Msa1012260:intron 30.0%
! ATAGTGGAGAAAATGAAACA+GGG - chr7_1:17368606-17368625 Msa1012260:CDS 30.0%
TCTTTCAATCACTACCAATT+TGG + chr7_1:17368796-17368815 None:intergenic 30.0%
GTTGTCCGATATCATATTAA+GGG + chr7_1:17368864-17368883 None:intergenic 30.0%
AGTTGTCCGATATCATATTA+AGG + chr7_1:17368865-17368884 None:intergenic 30.0%
!! TAAAATATTCGAGTTGGTAC+GGG + chr7_1:17369076-17369095 None:intergenic 30.0%
TCGATTGATTGCTAAATATC+CGG - chr7_1:17369190-17369209 Msa1012260:intron 30.0%
TAAATATCCGGTTGTATTGT+AGG - chr7_1:17369202-17369221 Msa1012260:intron 30.0%
AAATATCCGGTTGTATTGTA+GGG - chr7_1:17369203-17369222 Msa1012260:intron 30.0%
AGAAATAAGAGAAAGCTCTT+GGG + chr7_1:17369366-17369385 None:intergenic 30.0%
GTGAGAAAATTTGACAAAAG+AGG + chr7_1:17369409-17369428 None:intergenic 30.0%
TTCTATTGCAGTTTCTAAAC+CGG - chr7_1:17369551-17369570 Msa1012260:intron 30.0%
AATAACAACCTTACGAATGT+TGG - chr7_1:17369612-17369631 Msa1012260:intron 30.0%
ATATGCCAATCTCTGATGAA+AGG - chr7_1:17365553-17365572 Msa1012260:three_prime_UTR 35.0%
TTGAACCTTTCATCAGAGAT+TGG + chr7_1:17365561-17365580 None:intergenic 35.0%
! AATTAGATAGAGTGCTTTGG+TGG - chr7_1:17365579-17365598 Msa1012260:three_prime_UTR 35.0%
! ATTAGATAGAGTGCTTTGGT+GGG - chr7_1:17365580-17365599 Msa1012260:three_prime_UTR 35.0%
TACTTGCAGAATGTTCATCA+AGG - chr7_1:17365620-17365639 Msa1012260:three_prime_UTR 35.0%
GGAATTGTTGTCTATGAACT+TGG - chr7_1:17365822-17365841 Msa1012260:three_prime_UTR 35.0%
TGTGATTGCAGATGCTAAAA+AGG - chr7_1:17365941-17365960 Msa1012260:CDS 35.0%
GGATGAACTAATGAATGAAG+AGG - chr7_1:17365999-17366018 Msa1012260:intron 35.0%
TGAACTAATGAATGAAGAGG+AGG - chr7_1:17366002-17366021 Msa1012260:intron 35.0%
! GTCCTTTTTCTTCATTTTGC+TGG - chr7_1:17366113-17366132 Msa1012260:intron 35.0%
GACCAGCAAAATGAAGAAAA+AGG + chr7_1:17366118-17366137 None:intergenic 35.0%
! TTTTCTTCATTTTGCTGGTC+TGG - chr7_1:17366118-17366137 Msa1012260:intron 35.0%
! AATGTTGCTTCACCAACAAA+TGG + chr7_1:17366269-17366288 None:intergenic 35.0%
TTATTCCTCTTTGATAGGAG+TGG - chr7_1:17366293-17366312 Msa1012260:intron 35.0%
TTGAATCAGCATAGCTGATA+AGG + chr7_1:17366484-17366503 None:intergenic 35.0%
ACAAAGAATCTAACCTTCTC+TGG + chr7_1:17366615-17366634 None:intergenic 35.0%
GTTCTTATTGTCTGACAATG+AGG - chr7_1:17366939-17366958 Msa1012260:intron 35.0%
TCATGGTTAGCATTCAATGA+AGG - chr7_1:17367368-17367387 Msa1012260:intron 35.0%
ACAAAACTTTATCGGATCCT+AGG + chr7_1:17367773-17367792 None:intergenic 35.0%
ATTAGAGACAAGTTTCCTCA+CGG + chr7_1:17367835-17367854 None:intergenic 35.0%
GTCATTATTGAATGTACTGC+AGG + chr7_1:17368093-17368112 None:intergenic 35.0%
AAACCCTTCATACTTATCGA+TGG - chr7_1:17368240-17368259 Msa1012260:intron 35.0%
GCTCAAATATCTAATCCAAG+TGG + chr7_1:17368331-17368350 None:intergenic 35.0%
GATTAGATATTTGAGCCTTG+AGG - chr7_1:17368335-17368354 Msa1012260:intron 35.0%
AATTGACAGTTTAACCCTCA+AGG + chr7_1:17368353-17368372 None:intergenic 35.0%
! AATTGAAGGCACATATCAAC+AGG + chr7_1:17368438-17368457 None:intergenic 35.0%
! AACTTTTACACATCGTCTAC+AGG - chr7_1:17368484-17368503 Msa1012260:intron 35.0%
!! GTCTACAGGTTTGTTATGAT+TGG - chr7_1:17368498-17368517 Msa1012260:intron 35.0%
! CATAGTGGAGAAAATGAAAC+AGG - chr7_1:17368605-17368624 Msa1012260:CDS 35.0%
GCATACTGCATAGTTTGATT+TGG - chr7_1:17368737-17368756 Msa1012260:intron 35.0%
TTCAATCACTACCAATTTGG+AGG + chr7_1:17368793-17368812 None:intergenic 35.0%
! TGGTAGTGATTGAAAGACTA+CGG - chr7_1:17368799-17368818 Msa1012260:intron 35.0%
TTAAGGGAAATTACTACTGC+AGG + chr7_1:17368848-17368867 None:intergenic 35.0%
AAGAAAAGGATGACACGAAA+AGG + chr7_1:17368988-17369007 None:intergenic 35.0%
! CATCCTTTTCTTTACCTTAC+TGG - chr7_1:17368996-17369015 Msa1012260:intron 35.0%
TTTAGAAAGAAAGGCCAGTA+AGG + chr7_1:17369013-17369032 None:intergenic 35.0%
AATATCCGGTTGTATTGTAG+GGG - chr7_1:17369204-17369223 Msa1012260:intron 35.0%
TGCTAAAGATACCAGAAATC+TGG - chr7_1:17369246-17369265 Msa1012260:intron 35.0%
! GTTCTTTTGTCTTCAGAACT+GGG + chr7_1:17369289-17369308 None:intergenic 35.0%
CAGAAATAAGAGAAAGCTCT+TGG + chr7_1:17369367-17369386 None:intergenic 35.0%
GAGTAGGATGATTGTTAAAG+AGG - chr7_1:17369474-17369493 Msa1012260:intron 35.0%
TCTACTTACCAACATTCGTA+AGG + chr7_1:17369623-17369642 None:intergenic 35.0%
TGCTTTCAGAAACCGAATCA+AGG + chr7_1:17365660-17365679 None:intergenic 40.0%
!! TGATTCGGTTTCTGAAAGCA+AGG - chr7_1:17365660-17365679 Msa1012260:three_prime_UTR 40.0%
! GATTCGGTTTCTGAAAGCAA+GGG - chr7_1:17365661-17365680 Msa1012260:three_prime_UTR 40.0%
AAGGGAATGGAATGCTTCAA+AGG - chr7_1:17365679-17365698 Msa1012260:three_prime_UTR 40.0%
GCAGATGCTAAAAAGGATGA+AGG - chr7_1:17365948-17365967 Msa1012260:CDS 40.0%
AGTAGAAGAACAGCAAGAAG+AGG - chr7_1:17366029-17366048 Msa1012260:intron 40.0%
!!! CATTTTGCTGGTCTGGTTTT+TGG - chr7_1:17366125-17366144 Msa1012260:intron 40.0%
!! GTGGTTACTAGCTTTCTTTG+TGG - chr7_1:17366149-17366168 Msa1012260:intron 40.0%
!!! GTTTTTTTCCTGCTGTGTCT+AGG - chr7_1:17366212-17366231 Msa1012260:intron 40.0%
GAGATTCTACAAGAAGCTGA+TGG - chr7_1:17366549-17366568 Msa1012260:CDS 40.0%
AAGAATCTAACCTTCTCTGG+TGG + chr7_1:17366612-17366631 None:intergenic 40.0%
GGTCTCAATATTTGCTTGCA+AGG + chr7_1:17367063-17367082 None:intergenic 40.0%
GGACATTTGGTTACTTCCAA+AGG + chr7_1:17367301-17367320 None:intergenic 40.0%
TTAATCCCGGTGAAGAATAC+AGG - chr7_1:17367515-17367534 Msa1012260:intron 40.0%
TCCTGGGATTGTAGTTCAAT+CGG - chr7_1:17367691-17367710 Msa1012260:intron 40.0%
AACTTTATCGGATCCTAGGT+CGG + chr7_1:17367769-17367788 None:intergenic 40.0%
! TTGCCCGTATTTTTCACTGT+GGG - chr7_1:17367808-17367827 Msa1012260:intron 40.0%
TTGAATGTACTGCAGGGTAT+GGG + chr7_1:17368086-17368105 None:intergenic 40.0%
ATTGAATGTACTGCAGGGTA+TGG + chr7_1:17368087-17368106 None:intergenic 40.0%
GGTCCATCGATAAGTATGAA+GGG + chr7_1:17368246-17368265 None:intergenic 40.0%
AGGTCCATCGATAAGTATGA+AGG + chr7_1:17368247-17368266 None:intergenic 40.0%
! CATGATGTTTCTGCGAAATG+AGG + chr7_1:17368267-17368286 None:intergenic 40.0%
TATCTAATCCAAGTGGCAGA+TGG + chr7_1:17368324-17368343 None:intergenic 40.0%
AGTAATCCTCAAGAGCTTCA+TGG - chr7_1:17368635-17368654 Msa1012260:CDS 40.0%
GTAATCCTCAAGAGCTTCAT+GGG - chr7_1:17368636-17368655 Msa1012260:CDS 40.0%
CAAACTATGCAGTATGCTTG+AGG + chr7_1:17368734-17368753 None:intergenic 40.0%
!! TACTGCAGGCGATATTTTTG+GGG + chr7_1:17368834-17368853 None:intergenic 40.0%
! CTACTGCAGGCGATATTTTT+GGG + chr7_1:17368835-17368854 None:intergenic 40.0%
! ACTACTGCAGGCGATATTTT+TGG + chr7_1:17368836-17368855 None:intergenic 40.0%
! ACGAAAAGGATCAGTGGATT+TGG + chr7_1:17368974-17368993 None:intergenic 40.0%
AGGCCAGTAAGGTAAAGAAA+AGG + chr7_1:17369002-17369021 None:intergenic 40.0%
! CGTTCTTTTGTCTTCAGAAC+TGG + chr7_1:17369290-17369309 None:intergenic 40.0%
AGAAGATGCTTGACCTGATA+AGG + chr7_1:17369580-17369599 None:intergenic 40.0%
! GGTTTCTGAAAGCAAGGGAA+TGG - chr7_1:17365666-17365685 Msa1012260:three_prime_UTR 45.0%
ACACCAAAACTCAGCAACAC+AGG + chr7_1:17366095-17366114 None:intergenic 45.0%
TAGGAGTGGTGTGGTATCAT+AGG - chr7_1:17366307-17366326 Msa1012260:intron 45.0%
ATGGAATCATCTTGTCCCGT+GGG - chr7_1:17366568-17366587 Msa1012260:CDS 45.0%
ATCTTGTCCCGTGGGAATTT+GGG - chr7_1:17366576-17366595 Msa1012260:CDS 45.0%
ATCAATGCCCAAATTCCCAC+GGG + chr7_1:17366586-17366605 None:intergenic 45.0%
GATCAATGCCCAAATTCCCA+CGG + chr7_1:17366587-17366606 None:intergenic 45.0%
CATTGATCTTCCACCAGAGA+AGG - chr7_1:17366599-17366618 Msa1012260:CDS 45.0%
TGCACTGTGACTGTTAATCC+CGG - chr7_1:17367502-17367521 Msa1012260:intron 45.0%
TGAGCTCCTGTATTCTTCAC+CGG + chr7_1:17367524-17367543 None:intergenic 45.0%
ACCGATTGAACTACAATCCC+AGG + chr7_1:17367695-17367714 None:intergenic 45.0%
ACTTTATCGGATCCTAGGTC+GGG + chr7_1:17367768-17367787 None:intergenic 45.0%
CAGTGAAAAATACGGGCAAG+AGG + chr7_1:17367807-17367826 None:intergenic 45.0%
! CTTGCCCGTATTTTTCACTG+TGG - chr7_1:17367807-17367826 Msa1012260:intron 45.0%
! TGCCCGTATTTTTCACTGTG+GGG - chr7_1:17367809-17367828 Msa1012260:intron 45.0%
CGGTTCCATATATGCTTACG+CGG - chr7_1:17368112-17368131 Msa1012260:intron 45.0%
GCATACCGCGTAAGCATATA+TGG + chr7_1:17368120-17368139 None:intergenic 45.0%
TGGAGCAACTACTGCAAAGA+TGG - chr7_1:17368518-17368537 Msa1012260:intron 45.0%
AGCAACTACTGCAAAGATGG+TGG - chr7_1:17368521-17368540 Msa1012260:intron 45.0%
!! ACTGCAAAGATGGTGGATCT+TGG - chr7_1:17368528-17368547 Msa1012260:intron 45.0%
! ACCAGCTTTGCAGTGGATTT+AGG + chr7_1:17368569-17368588 None:intergenic 45.0%
ATCATTGACCAGCTTTGCAG+TGG + chr7_1:17368576-17368595 None:intergenic 45.0%
AACTTCCCATGAAGCTCTTG+AGG + chr7_1:17368644-17368663 None:intergenic 45.0%
TACACGATCAACAGGTCCAA+CGG + chr7_1:17368693-17368712 None:intergenic 45.0%
GGAGTAGATACACGATCAAC+AGG + chr7_1:17368701-17368720 None:intergenic 45.0%
GCAGTATGCTTGAGGATTAC+CGG + chr7_1:17368726-17368745 None:intergenic 45.0%
GATGACACGAAAAGGATCAG+TGG + chr7_1:17368980-17368999 None:intergenic 45.0%
ATGTGCCCCTACAATACAAC+CGG + chr7_1:17369212-17369231 None:intergenic 45.0%
! TGTATTGTAGGGGCACATCA+AGG - chr7_1:17369214-17369233 Msa1012260:intron 45.0%
TGCTTGAACAGCCAGATTTC+TGG + chr7_1:17369260-17369279 None:intergenic 45.0%
ATTATGTGGGGAGCAAGAGT+AGG - chr7_1:17369458-17369477 Msa1012260:intron 45.0%
TCTAAACCGGTCACCTTATC+AGG - chr7_1:17369564-17369583 Msa1012260:intron 45.0%
!! CAGCCTGTGTTGCTGAGTTT+TGG - chr7_1:17366089-17366108 Msa1012260:intron 50.0%
!!! TGCTGGTCTGGTTTTTGGTG+TGG - chr7_1:17366130-17366149 Msa1012260:intron 50.0%
CCACACCACTCCTATCAAAG+AGG + chr7_1:17366301-17366320 None:intergenic 50.0%
CCTCTTTGATAGGAGTGGTG+TGG - chr7_1:17366298-17366317 Msa1012260:intron 50.0%
GATGGAATCATCTTGTCCCG+TGG - chr7_1:17366567-17366586 Msa1012260:CDS 50.0%
CATCTTGTCCCGTGGGAATT+TGG - chr7_1:17366575-17366594 Msa1012260:CDS 50.0%
GAGCTCCTGTATTCTTCACC+GGG + chr7_1:17367523-17367542 None:intergenic 50.0%
GGCCCCACAGTGAAAAATAC+GGG + chr7_1:17367814-17367833 None:intergenic 50.0%
CGGCCCCACAGTGAAAAATA+CGG + chr7_1:17367815-17367834 None:intergenic 50.0%
GGGTGGTTCCATTTCTGTCT+AGG - chr7_1:17367911-17367930 Msa1012260:intron 50.0%
GCTTAGATCCATCTGCCACT+TGG - chr7_1:17368313-17368332 Msa1012260:intron 50.0%
GCCTAAATCCACTGCAAAGC+TGG - chr7_1:17368565-17368584 Msa1012260:intron 50.0%
CATGGGAAGTTGTCTGCTAC+TGG - chr7_1:17368653-17368672 Msa1012260:CDS 50.0%
GATCGTGTATCTACTCCAGC+CGG - chr7_1:17368704-17368723 Msa1012260:intron 50.0%
TATGCTTGAGGATTACCGGC+TGG + chr7_1:17368722-17368741 None:intergenic 50.0%
TTGGTACGGGCAAGGTCTAA+AGG + chr7_1:17369063-17369082 None:intergenic 50.0%
!! TATTCGAGTTGGTACGGGCA+AGG + chr7_1:17369071-17369090 None:intergenic 50.0%
GCTTGACCTGATAAGGTGAC+CGG + chr7_1:17369573-17369592 None:intergenic 50.0%
TTCCTGCTGTGTCTAGGTGC+AGG - chr7_1:17366218-17366237 Msa1012260:intron 55.0%
TCCTGCTGTGTCTAGGTGCA+GGG - chr7_1:17366219-17366238 Msa1012260:intron 55.0%
TGTGTCTAGGTGCAGGGTCT+GGG - chr7_1:17366225-17366244 Msa1012260:intron 55.0%
! TTTTTCACTGTGGGGCCGTG+AGG - chr7_1:17367817-17367836 Msa1012260:intron 55.0%
GATCAACAGGTCCAACGGCA+CGG + chr7_1:17368688-17368707 None:intergenic 55.0%
ACCCTGCACCTAGACACAGC+AGG + chr7_1:17366223-17366242 None:intergenic 60.0%
CTGTGTCTAGGTGCAGGGTC+TGG - chr7_1:17366224-17366243 Msa1012260:intron 60.0%
CAGCTTCTGCCATGACAGGG+CGG + chr7_1:17367725-17367744 None:intergenic 60.0%
CCGCAGCTTCTGCCATGACA+GGG + chr7_1:17367728-17367747 None:intergenic 60.0%
CCCTGTCATGGCAGAAGCTG+CGG - chr7_1:17367725-17367744 Msa1012260:intron 60.0%
CCTGTCATGGCAGAAGCTGC+GGG - chr7_1:17367726-17367745 Msa1012260:intron 60.0%
ATCCTAGGTCGGGACTGACG+GGG + chr7_1:17367758-17367777 None:intergenic 60.0%
GATCCTAGGTCGGGACTGAC+GGG + chr7_1:17367759-17367778 None:intergenic 60.0%
GGATCCTAGGTCGGGACTGA+CGG + chr7_1:17367760-17367779 None:intergenic 60.0%
!! GTCAGAGCACCGCCCTGTCA+TGG - chr7_1:17367713-17367732 Msa1012260:intron 65.0%
CCCGCAGCTTCTGCCATGAC+AGG + chr7_1:17367729-17367748 None:intergenic 65.0%
! GGCAGTTCTAGCCGTGCCGT+TGG - chr7_1:17368674-17368693 Msa1012260:intron 65.0%
AGCCCCGTCAGTCCCGACCT+AGG - chr7_1:17367753-17367772 Msa1012260:intron 70.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 17365511 17369701 17365511 ID=Msa1012260;Name=Msa1012260
chr7_1 mRNA 17365511 17369701 17365511 ID=Msa1012260-mRNA-1;Parent=Msa1012260;Name=Msa1012260-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0|0.2|0.16|0.33|0.8|1|6|386|188
chr7_1 exon 17365511 17365988 17365511 ID=Msa1012260-mRNA-1:exon:4359;Parent=Msa1012260-mRNA-1
chr7_1 exon 17366487 17366706 17366487 ID=Msa1012260-mRNA-1:exon:4358;Parent=Msa1012260-mRNA-1
chr7_1 exon 17367079 17367150 17367079 ID=Msa1012260-mRNA-1:exon:4357;Parent=Msa1012260-mRNA-1
chr7_1 exon 17368592 17368681 17368592 ID=Msa1012260-mRNA-1:exon:4356;Parent=Msa1012260-mRNA-1
chr7_1 exon 17368893 17368978 17368893 ID=Msa1012260-mRNA-1:exon:4355;Parent=Msa1012260-mRNA-1
chr7_1 exon 17369695 17369701 17369695 ID=Msa1012260-mRNA-1:exon:4354;Parent=Msa1012260-mRNA-1
chr7_1 CDS 17369695 17369701 17369695 ID=Msa1012260-mRNA-1:cds;Parent=Msa1012260-mRNA-1
chr7_1 CDS 17368893 17368978 17368893 ID=Msa1012260-mRNA-1:cds;Parent=Msa1012260-mRNA-1
chr7_1 CDS 17368592 17368681 17368592 ID=Msa1012260-mRNA-1:cds;Parent=Msa1012260-mRNA-1
chr7_1 CDS 17367079 17367150 17367079 ID=Msa1012260-mRNA-1:cds;Parent=Msa1012260-mRNA-1
chr7_1 CDS 17366487 17366706 17366487 ID=Msa1012260-mRNA-1:cds;Parent=Msa1012260-mRNA-1
chr7_1 CDS 17365897 17365988 17365897 ID=Msa1012260-mRNA-1:cds;Parent=Msa1012260-mRNA-1
chr7_1 three_prime_UTR 17365511 17365896 17365511 ID=Msa1012260-mRNA-1:three_prime_utr;Parent=Msa1012260-mRNA-1
Gene Sequence

>Msa1012260

ATGGCAGGTGCAGGGTCTGGGTTAATATTAAACATGTTGCCATTTGTTGGTGAAGCAACATTTAGCTTATTCCTCTTTGATAGGAGTGGTGTGGGATTGACCCATTTTGATGAGATTCTACAAGAAGCTGATGGAATCATCTTGTCCCGTGGGAATTTGGGCATTGATCTTCCACCAGAGAAGAATATGCAGAGGTGTAGTCAGCCCATACCCTGCAGTACATTCAATAATGACGGTTCCATATATGCTTACGCGGTTTGTTATGATTGGAGCAACTACTGCAAAGATGGTGGATCTTGGTCGACAACATTCTCGCCTAAATCCACTGCAAAGCTGGTCAATGATAGTAACTTGCATAGTGGAGAAAATGAAACAGGGTTAGTGAGTAATCCTCAAGAGCTTCATGGGAAGTTGTCTGCTACTGGCAGTTCTAGCCGTGCCGTTGGACCTGTTGATCGTGTATCTACTCCAGCCGGGGCACATCAAGGAAGCATTTTTGCTAAAGATACCAGAAATCTGGCTGTTCAAGCAGCAGATCCCAGTTCTGAAGACAAAAGAACGCAGTGA
Protein sequence

>Msa1012260

MAGAGSGLILNMLPFVGEATFSLFLFDRSGVGLTHFDEILQEADGIILSRGNLGIDLPPEKNMQRCSQPIPCSTFNNDGSIYAYAVCYDWSNYCKDGGSWSTTFSPKSTAKLVNDSNLHSGENETGLVSNPQELHGKLSATGSSSRAVGPVDRVSTPAGAHQGSIFAKDTRNLAVQAADPSSEDKRTQ*