AlfalfaGEDB | Msa0274200

M. sativa cultivar zhongmu-4 / Msa0274200

KEGG: -
GO: GO:0002376 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008219 GO:0009626 GO:0009987 GO:0010337 GO:0010565 GO:0010817 GO:0012501 GO:0019222 GO:0031323 GO:0032350 GO:0033554 GO:0034050 GO:0045087 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0062012 GO:0065007 GO:0065008
PFAM: PF01823.22

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0274200	XP_003593800.1	99.293	566	4	0	1	566	1	566	0.0	1168

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0274200	sp\|Q9C7N2\|CAD1_ARATH	74.120	568	136	2	3	566	1	561	0.0	865

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0041880	Msa0274200	0.802530	5.371067e-49	-8.615850e-47
Msa0274200	Msa0314370	0.975549	3.711118e-140	-8.615850e-47
Msa0274200	Msa1087310	0.989109	9.869797e-177	-8.615850e-47
Msa0274200	Msa1111770	0.803515	3.360767e-49	-8.615850e-47
Msa0274200	Msa1439370	0.996647	2.751032e-230	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0274200	MtrunA17_Chr2g0284691	99.293	566	4	0	1	566	1	566	0.0	1168
Msa0274200	MtrunA17_Chr4g0013011	60.381	578	197	4	18	566	10	584	0.0	722
Msa0274200	MtrunA17_Chr2g0277061	43.697	595	269	11	22	565	15	594	9.95e-155	456
Msa0274200	MtrunA17_Chr8g0377631	39.251	614	298	9	22	566	11	618	3.19e-139	417
Msa0274200	MtrunA17_Chr8g0385121	39.835	605	295	11	11	564	2	588	2.61e-133	401
Msa0274200	MtrunA17_Chr3g0122211	38.736	617	292	13	22	566	17	619	2.71e-132	399
Msa0274200	MtrunA17_Chr8g0391931	40.772	596	286	15	22	565	9	589	1.76e-131	396
Msa0274200	MtrunA17_Chr4g0046841	38.907	604	291	12	22	564	25	611	4.96e-131	396
Msa0274200	MtrunA17_Chr8g0385131	37.786	569	312	10	22	566	16	566	7.32e-120	365
Msa0274200	MtrunA17_Chr8g0385181	39.464	560	302	13	22	566	16	553	1.41e-118	362

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0274200	AT1G29690.1	74.120	568	136	2	3	566	1	561	0.0	865
Msa0274200	AT4G24290.2	42.088	594	277	11	22	564	14	591	4.82e-146	434
Msa0274200	AT4G24290.3	42.088	594	277	11	22	564	14	591	4.82e-146	434
Msa0274200	AT1G28380.1	39.866	597	296	11	22	565	18	604	8.41e-134	403
Msa0274200	AT1G14780.1	39.060	617	300	12	19	565	9	619	2.39e-130	395
Msa0274200	AT4G24290.1	37.771	323	171	4	22	314	14	336	6.29e-67	221

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Find 163 sgRNAs with CRISPR-Local

Find 472 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTAGCTTACCTAGCCA+AGG	0.588224	2_3:+5993465	None:intergenic
AAAGGGCCGCAAGATATTCC+TGG	0.476025	2_3:-5989748	Msa0274200:CDS
AAATACTGTAGGTCTTCAAT+AGG	0.446642	2_3:+5991130	None:intergenic
AACCATGGCAAGTGATTTAG+TGG	0.455369	2_3:+5993588	None:intergenic
AACTGAAGAGATTGACAAAC+AGG	0.408326	2_3:+5991046	None:intergenic
AACTTCATGTTAATAATGTC+TGG	0.460930	2_3:+5991660	None:intergenic
AATAACTCGAGTTTGGGCGC+CGG	0.628765	2_3:-5991095	Msa0274200:CDS
AATCGGTGCAGTGCTTACAT+GGG	0.651985	2_3:+5990071	None:intergenic
AATGAAAGGTCAGGCATAGA+AGG	0.419511	2_3:-5993679	Msa0274200:CDS
AATTCTATGTTCAACTTCAC+TGG	0.404887	2_3:-5993634	Msa0274200:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAATTGTTGAATGAATAGGT+TGG	+	chr2_3:5989468-5989487	None:intergenic	30.0%
ATTAGGCTTCTTTACTGTAA+AGG	-	chr2_3:5989664-5989683	Msa0274200:CDS	30.0%
TTAGGCTTCTTTACTGTAAA+GGG	-	chr2_3:5989665-5989684	Msa0274200:CDS	30.0%
TGTTGTTCCTAATGTTTCTT+TGG	-	chr2_3:5989756-5989775	Msa0274200:CDS	30.0%
CAAATCCTTACCTTACAAAT+CGG	-	chr2_3:5990434-5990453	Msa0274200:intron	30.0%
ATCACATTGTTGATGTTGTA+AGG	-	chr2_3:5990500-5990519	Msa0274200:intron	30.0%
ATTGTTGATGTTGTAAGGAA+TGG	-	chr2_3:5990505-5990524	Msa0274200:intron	30.0%
CATATGATATGAGAGATAAC+TGG	+	chr2_3:5990621-5990640	None:intergenic	30.0%
CATGCACTTTAAATTGCAAT+AGG	+	chr2_3:5990684-5990703	None:intergenic	30.0%
ACTTACAGTGGTTATGTTAT+TGG	-	chr2_3:5990892-5990911	Msa0274200:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_3	gene	5989432	5996111	5989432	ID=Msa0274200;Name=Msa0274200
chr2_3	mRNA	5989432	5996111	5989432	ID=Msa0274200-mRNA-1;Parent=Msa0274200;Name=Msa0274200-mRNA-1;_AED=0.10;_eAED=0.10;_QI=127\|1\|1\|1\|1\|1\|6\|231\|566
chr2_3	exon	5989432	5990307	5989432	ID=Msa0274200-mRNA-1:exon:2769;Parent=Msa0274200-mRNA-1
chr2_3	exon	5991006	5991160	5991006	ID=Msa0274200-mRNA-1:exon:2768;Parent=Msa0274200-mRNA-1
chr2_3	exon	5991583	5991766	5991583	ID=Msa0274200-mRNA-1:exon:2767;Parent=Msa0274200-mRNA-1
chr2_3	exon	5992247	5992442	5992247	ID=Msa0274200-mRNA-1:exon:2766;Parent=Msa0274200-mRNA-1
chr2_3	exon	5993478	5993716	5993478	ID=Msa0274200-mRNA-1:exon:2765;Parent=Msa0274200-mRNA-1
chr2_3	exon	5995703	5996111	5995703	ID=Msa0274200-mRNA-1:exon:2764;Parent=Msa0274200-mRNA-1
chr2_3	five_prime_UTR	5995985	5996111	5995985	ID=Msa0274200-mRNA-1:five_prime_utr;Parent=Msa0274200-mRNA-1
chr2_3	CDS	5995703	5995984	5995703	ID=Msa0274200-mRNA-1:cds;Parent=Msa0274200-mRNA-1
chr2_3	CDS	5993478	5993716	5993478	ID=Msa0274200-mRNA-1:cds;Parent=Msa0274200-mRNA-1
chr2_3	CDS	5992247	5992442	5992247	ID=Msa0274200-mRNA-1:cds;Parent=Msa0274200-mRNA-1
chr2_3	CDS	5991583	5991766	5991583	ID=Msa0274200-mRNA-1:cds;Parent=Msa0274200-mRNA-1
chr2_3	CDS	5991006	5991160	5991006	ID=Msa0274200-mRNA-1:cds;Parent=Msa0274200-mRNA-1
chr2_3	CDS	5989663	5990307	5989663	ID=Msa0274200-mRNA-1:cds;Parent=Msa0274200-mRNA-1
chr2_3	three_prime_UTR	5989432	5989662	5989432	ID=Msa0274200-mRNA-1:three_prime_utr;Parent=Msa0274200-mRNA-1

Gene Sequence

>Msa0274200

ATGATTATGGAAAACACAACTTCAACAACAAGCTCTGATTCTCTTTCTGCAACAATTTGCAACTCAATCCAAGCTTTGGGTCGTGGTTTTGATGTTACATCAGATATTAGGCTTCTTTACTGTAAAGGGGCACCTGGGTCTAGGCTTGTTCATCTCGATGAGGAACATAATAGGGATCTTGCTCTTTCTCAAGAACTTGTTGTTCCTAATGTTTCTTTGGATATTGATTTCTCTCGTGGGAAAAGTGGTATTGAGAAGACCCCTGTTTGTAGTTTTGAAAAGATGGCAGAATATTTCAATGAAAGGTCAGGCATAGAAGGAAAAATTCCACTTGGAAGCTTCAATTCTATGTTCAACTTCACTGGCTCTTCAATGGTTGATGCAGCAGCCACTAAATCACTTGCCATGGTTGGATATTTCATTCCACTATTCGAAGTTAAATTAACAAAACAAAATTTGGCCTTGAATGATGAAGTCAGGCGCGCTGTTCCTTACTCTTGGGATCCAGCATCCTTGGCTAGTTTTATTGAGAATTATGGTACCCATATTGTTACATCTGCAACAGTTGGTGGCAGAGACGTCGTGTATATTAGGCAGCACCAATCATCTTCTTTGTCTGCACCGGACATTGAGAACTATGTTAAGGACATTGAAAATGATAGGTTTCTTGATGCAAAAAACACATCAGGACCCGCTGCTTTGAAGTACAAAGAGAAGGATGTTACAGTCATTTTTAGAAGAAGAGGAGGGGATGATCTTGAGCAAAGTCATATTAAATGGGTGGAAACTGTAAAATTGGCGCCAGACATTATTAACATGAAGTTTACACCCATCGTTTCTCTTCTTGAAGGAGTACATGGCGTAAAGCTTTTGGAACGTGCTATTGATCTATATCTTCAGTACAAACCTCCTATTGAAGACCTACAGTATTTCTTGGATTTCCAAATAACTCGAGTTTGGGCGCCGGAGCAGAATAATCTACAAAGAAAGGAGCCTGTTTGTCAATCTCTTCAGTTCAGCTTAATGGGACCTAAGCTTTTTGTCAGTCCAGATCAGGTAACAGTTGGACGCAAGCCTGTGACCGGACTAAGGCTGAGCCTAGAAGGCAACAAACAGAACCGACTCGCAATTCATTTGCAGCATTTAGTATCACTCCCGAAAAACCTCCAACCACATTGGGATGCACACATGGCCATAGGTGCTCCCAAATGGCAAGGTCCGGAGGAGCAAGACAGCCGTTGGTTTGAACCGATCAAATGGAAGAACTTCTCCCATGTAAGCACTGCACCGATTGAGATAACTGAAACCAACATTGGGGACCTCTCTGGTGTTCATATTGTCACTGGGGCCCAACTCGGCGTTTGGGACTTCGGTGCCAAAAATGTATTACACCTCAAACTACTTTTCTCTAAGGTACCAGGGTGCACGATAAGACGGTCAGTGTGGGATCATAACCCTTCCACTCCGGCAGCTGCACACAAATCAGACGGTGCTTCGTCATCATCGGCGAAGAAAACCACTGACGAGAAAAAGGAAGATAGTTCCGTCCACATTGGAAAACTCGCGAAAATTGTGGACATGACAGAAATGTCAAAAGGGCCGCAAGATATTCCTGGTCATTGGTTAGTTACAGGGGCTAAGCTTGGAGTAGAAAAAGGAAAAATTGTACTCAGGATAAAATACTCCTTGCTAAACTATTGA

Protein sequence

>Msa0274200

MIMENTTSTTSSDSLSATICNSIQALGRGFDVTSDIRLLYCKGAPGSRLVHLDEEHNRDLALSQELVVPNVSLDIDFSRGKSGIEKTPVCSFEKMAEYFNERSGIEGKIPLGSFNSMFNFTGSSMVDAAATKSLAMVGYFIPLFEVKLTKQNLALNDEVRRAVPYSWDPASLASFIENYGTHIVTSATVGGRDVVYIRQHQSSSLSAPDIENYVKDIENDRFLDAKNTSGPAALKYKEKDVTVIFRRRGGDDLEQSHIKWVETVKLAPDIINMKFTPIVSLLEGVHGVKLLERAIDLYLQYKPPIEDLQYFLDFQITRVWAPEQNNLQRKEPVCQSLQFSLMGPKLFVSPDQVTVGRKPVTGLRLSLEGNKQNRLAIHLQHLVSLPKNLQPHWDAHMAIGAPKWQGPEEQDSRWFEPIKWKNFSHVSTAPIEITETNIGDLSGVHIVTGAQLGVWDFGAKNVLHLKLLFSKVPGCTIRRSVWDHNPSTPAAAHKSDGASSSSAKKTTDEKKEDSSVHIGKLAKIVDMTEMSKGPQDIPGHWLVTGAKLGVEKGKIVLRIKYSLLNY*