AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0277950

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0277950 MtrunA17_Chr2g0291021 97.273 330 9 0 1 330 1 330 0.0 663
Msa0277950 MtrunA17_Chr7g0254981 73.050 141 37 1 49 188 50 190 8.39e-69 216
Msa0277950 MtrunA17_Chr7g0261911 60.390 154 52 3 33 186 252 396 6.97e-53 181
Msa0277950 MtrunA17_Chr1g0175091 48.087 183 77 3 35 217 244 408 1.95e-51 176
Msa0277950 MtrunA17_Chr1g0190301 53.216 171 73 3 33 200 156 322 1.24e-48 167
Msa0277950 MtrunA17_Chr7g0258551 51.351 148 69 2 46 193 209 353 2.99e-47 164
Msa0277950 MtrunA17_Chr7g0274471 53.333 150 68 1 48 197 217 364 1.35e-46 163
Msa0277950 MtrunA17_Chr8g0356501 59.398 133 51 2 49 179 37 168 4.32e-44 154
Msa0277950 MtrunA17_Chr5g0409731 52.448 143 58 1 49 181 25 167 6.24e-44 152
Msa0277950 MtrunA17_Chr4g0042451 44.792 192 80 6 47 220 15 198 2.14e-43 152
Msa0277950 MtrunA17_Chr2g0310011 58.779 131 47 2 49 175 35 162 4.51e-42 149
Msa0277950 MtrunA17_Chr7g0242521 46.497 157 75 4 49 200 13 165 5.75e-41 143
Msa0277950 MtrunA17_Chr5g0417521 44.262 183 87 5 47 217 13 192 7.39e-41 146
Msa0277950 MtrunA17_Chr2g0321461 49.682 157 61 3 47 185 43 199 9.79e-41 146
Msa0277950 MtrunA17_Chr6g0461641 48.701 154 63 2 47 185 40 192 1.19e-40 146
Msa0277950 MtrunA17_Chr2g0290991 47.826 138 56 2 49 173 39 173 2.40e-34 127
Msa0277950 MtrunA17_Chr1g0197171 47.059 136 63 2 49 176 44 178 7.00e-33 123
Msa0277950 MtrunA17_Chr1g0175071 46.043 139 66 2 49 184 177 309 1.08e-30 118
Msa0277950 MtrunA17_Chr1g0175081 48.000 75 39 0 35 109 8 82 2.97e-22 89.7
Msa0277950 MtrunA17_Chr7g0258531 37.222 180 94 5 13 180 171 343 7.52e-22 94.7
Msa0277950 MtrunA17_Chr2g0288601 66.071 56 19 0 51 106 73 128 1.03e-16 75.5
Msa0277950 MtrunA17_Chr7g0243081 61.404 57 22 0 51 107 73 129 1.49e-16 79.3
Msa0277950 MtrunA17_Chr6g0479431 60.000 65 24 1 51 113 204 268 2.58e-16 79.0
Msa0277950 MtrunA17_Chr8g0366281 46.535 101 44 3 51 151 175 265 2.77e-16 78.6
Msa0277950 MtrunA17_Chr3g0095611 48.718 78 24 1 51 112 21 98 7.91e-16 75.1
Msa0277950 MtrunA17_Chr1g0198601 56.061 66 29 0 49 114 211 276 1.48e-15 76.6
Msa0277950 MtrunA17_Chr5g0410741 63.636 55 20 0 51 105 221 275 2.24e-15 76.3
Msa0277950 MtrunA17_Chr8g0379121 63.636 55 20 0 51 105 113 167 2.34e-15 76.6
Msa0277950 MtrunA17_Chr1g0162931 53.425 73 33 1 51 123 20 91 2.89e-15 75.1
Msa0277950 MtrunA17_Chr3g0109981 63.636 55 20 0 51 105 276 330 3.19e-15 76.3
Msa0277950 MtrunA17_Chr2g0320231 65.455 55 19 0 51 105 99 153 4.52e-15 75.5
Msa0277950 MtrunA17_Chr1g0154261 61.818 55 21 0 51 105 128 182 5.88e-15 74.3
Msa0277950 MtrunA17_Chr1g0210911 65.385 52 18 0 51 102 24 75 6.45e-15 74.7
Msa0277950 MtrunA17_Chr5g0402631 55.714 70 30 1 49 117 214 283 4.10e-14 72.4
Msa0277950 MtrunA17_Chr2g0283951 67.347 49 16 0 51 99 91 139 1.46e-13 70.9
Msa0277950 MtrunA17_Chr5g0422621 58.182 55 23 0 49 103 198 252 5.22e-13 68.9
Msa0277950 MtrunA17_Chr4g0062921 48.718 78 39 1 39 115 224 301 6.08e-13 69.3
Msa0277950 MtrunA17_Chr4g0009981 50.685 73 33 2 41 113 108 177 1.01e-12 67.4
Msa0277950 MtrunA17_Chr4g0045361 60.000 55 22 0 49 103 274 328 1.17e-12 68.2
Msa0277950 MtrunA17_Chr4g0009901 56.364 55 23 1 49 103 168 221 1.98e-12 66.6
Msa0277950 MtrunA17_Chr4g0031961 48.611 72 35 2 49 119 131 201 7.83e-12 65.1
Msa0277950 MtrunA17_Chr2g0324891 50.820 61 29 1 42 102 154 213 9.01e-12 65.5
Msa0277950 MtrunA17_Chr5g0400241 43.038 79 44 1 27 105 289 366 1.27e-11 65.5
Msa0277950 MtrunA17_Chr7g0255711 58.182 55 22 1 49 103 91 144 1.33e-11 64.3
Msa0277950 MtrunA17_Chr4g0009851 52.308 65 28 2 49 113 169 230 2.08e-11 63.9
Msa0277950 MtrunA17_Chr2g0304841 54.545 55 24 1 49 103 213 266 2.56e-11 64.7
Msa0277950 MtrunA17_Chr4g0067981 58.182 55 22 1 49 103 215 268 3.46e-11 64.3
Msa0277950 MtrunA17_Chr4g0040481 53.704 54 24 1 49 102 158 210 4.84e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0277950 AT2G01060.1 66.779 298 69 9 43 330 9 286 3.04e-119 345
Msa0277950 AT2G01060.2 62.646 257 66 9 84 330 1 237 1.80e-88 265
Msa0277950 AT5G29000.1 57.692 156 63 2 29 183 170 323 2.09e-51 174
Msa0277950 AT5G29000.3 57.692 156 63 2 29 183 170 323 2.09e-51 174
Msa0277950 AT5G29000.2 57.692 156 63 2 29 183 213 366 4.06e-51 175
Msa0277950 AT3G04450.4 52.273 176 76 3 44 212 193 367 7.56e-51 174
Msa0277950 AT3G04450.2 52.273 176 76 3 44 212 193 367 7.56e-51 174
Msa0277950 AT3G04450.1 52.273 176 76 3 44 212 233 407 1.34e-50 174
Msa0277950 AT4G28610.1 52.432 185 80 4 41 225 217 393 4.26e-50 172
Msa0277950 AT5G06800.1 57.534 146 57 3 37 182 182 322 6.49e-50 171
Msa0277950 AT4G13640.1 59.574 141 47 2 49 179 37 177 1.91e-49 167
Msa0277950 AT3G13040.3 50.265 189 86 4 44 227 236 421 1.98e-49 171
Msa0277950 AT3G13040.1 50.265 189 86 4 44 227 236 421 1.98e-49 171
Msa0277950 AT3G13040.2 50.265 189 86 4 44 227 236 421 1.98e-49 171
Msa0277950 AT3G24120.1 58.156 141 49 2 49 179 41 181 7.71e-49 166
Msa0277950 AT5G06800.2 55.705 149 60 3 37 182 182 327 7.97e-49 168
Msa0277950 AT1G79430.2 61.268 142 47 3 49 185 34 172 2.76e-48 166
Msa0277950 AT4G13640.2 58.333 144 47 3 49 179 37 180 3.08e-47 161
Msa0277950 AT3G24120.2 56.944 144 49 3 49 179 41 184 9.96e-47 160
Msa0277950 AT3G04030.3 48.333 180 72 4 23 185 19 194 8.16e-43 152
Msa0277950 AT1G69580.2 49.693 163 65 3 47 192 32 194 9.30e-43 151
Msa0277950 AT2G20400.2 53.165 158 71 2 49 206 231 385 1.04e-42 152
Msa0277950 AT1G69580.1 49.091 165 62 4 47 192 32 193 1.05e-42 150
Msa0277950 AT2G20400.1 53.797 158 69 2 49 206 231 384 1.33e-42 152
Msa0277950 AT5G18240.1 51.316 152 61 1 47 185 43 194 4.04e-42 150
Msa0277950 AT5G18240.4 51.316 152 61 1 47 185 43 194 4.04e-42 150
Msa0277950 AT3G04030.2 47.778 180 72 5 23 185 19 193 3.73e-40 145
Msa0277950 AT5G18240.5 50.000 152 61 2 47 185 43 192 7.33e-39 142
Msa0277950 AT5G45580.1 45.223 157 56 3 49 182 7 156 4.34e-37 134
Msa0277950 AT3G04030.1 45.556 180 71 5 23 185 19 188 6.82e-37 136
Msa0277950 AT5G45580.2 45.860 157 55 3 49 182 23 172 8.00e-37 134
Msa0277950 AT5G18240.2 48.026 152 60 2 47 185 43 188 3.20e-36 135
Msa0277950 AT5G18240.3 48.026 152 60 2 47 185 43 188 3.20e-36 135
Msa0277950 AT3G12730.1 47.826 138 64 4 49 179 23 159 3.09e-35 128
Msa0277950 AT1G79430.1 54.545 110 42 3 81 185 1 107 4.73e-27 108
Msa0277950 AT5G29000.4 60.674 89 33 1 29 117 213 299 3.09e-25 103
Msa0277950 AT3G04450.3 64.286 70 25 0 44 113 233 302 3.48e-25 103
Msa0277950 AT3G04030.5 49.153 118 47 2 81 185 1 118 1.34e-24 102
Msa0277950 AT5G06800.3 60.563 71 26 1 45 115 188 256 5.54e-24 99.8
Msa0277950 AT3G04030.4 44.915 118 46 3 81 185 1 112 4.54e-19 86.7
Msa0277950 AT5G18240.6 43.220 118 48 2 81 185 1 112 5.52e-19 86.7
Msa0277950 AT2G06020.1 59.649 57 23 0 49 105 86 142 7.10e-18 83.2
Msa0277950 AT2G42660.1 65.517 58 20 0 51 108 52 109 2.94e-17 80.5
Msa0277950 AT2G40260.1 57.333 75 20 1 51 125 84 146 3.08e-17 82.4
Msa0277950 AT4G04605.1 69.231 52 16 0 51 102 16 67 4.16e-17 79.3
Msa0277950 AT1G32240.1 43.396 106 51 2 51 156 215 311 1.79e-16 80.1
Msa0277950 AT1G32240.2 43.396 106 51 2 51 156 215 311 1.99e-16 79.7
Msa0277950 AT5G42630.2 57.576 66 28 0 51 116 107 172 4.46e-16 76.6
Msa0277950 AT5G42630.1 57.576 66 28 0 51 116 107 172 5.78e-16 77.4
Msa0277950 AT3G12730.2 39.623 106 56 4 81 179 1 105 1.29e-15 74.3
Msa0277950 AT5G16560.1 60.000 65 24 1 51 113 221 285 1.62e-15 77.4
Msa0277950 AT1G14600.1 69.231 52 16 0 51 102 25 76 2.03e-15 75.5
Msa0277950 AT4G17695.1 63.636 55 20 0 51 105 166 220 3.12e-15 75.9
Msa0277950 AT2G02060.2 69.231 52 16 0 51 102 31 82 3.28e-15 74.7
Msa0277950 AT2G02060.1 69.231 52 16 0 51 102 31 82 3.28e-15 74.7
Msa0277950 AT2G38300.1 63.636 55 20 0 51 105 56 110 3.76e-15 75.9
Msa0277950 AT4G37180.1 60.000 55 22 0 49 103 210 264 3.44e-14 73.2
Msa0277950 AT4G37180.2 60.000 55 22 0 49 103 217 271 4.20e-14 72.8
Msa0277950 AT4G04580.1 54.545 55 25 0 51 105 16 70 1.20e-13 68.6
Msa0277950 AT1G49560.1 54.545 66 30 0 49 114 192 257 1.52e-13 70.9
Msa0277950 AT3G25790.1 58.182 55 23 0 54 108 201 255 2.98e-13 70.1
Msa0277950 AT1G25550.1 55.385 65 29 0 44 108 204 268 5.27e-13 69.3
Msa0277950 AT3G10760.1 48.101 79 40 1 25 103 81 158 7.96e-13 68.9
Msa0277950 AT1G13300.1 44.086 93 41 2 16 108 159 240 1.40e-12 68.2
Msa0277950 AT1G68670.1 48.052 77 40 0 42 118 208 284 1.82e-12 67.8
Msa0277950 AT5G05090.1 60.377 53 20 1 51 103 83 134 4.25e-12 65.9
Msa0277950 AT4G37180.3 55.172 58 26 0 49 106 210 267 6.17e-12 65.5
Msa0277950 AT5G58080.1 52.174 69 28 3 49 117 194 257 7.07e-12 66.6
Msa0277950 AT5G59570.2 58.491 53 21 1 51 103 143 194 9.86e-12 65.1
Msa0277950 AT5G59570.1 58.491 53 21 1 51 103 143 194 9.86e-12 65.1
Msa0277950 AT3G46640.2 58.491 53 21 1 51 103 146 197 1.21e-11 65.1
Msa0277950 AT3G46640.1 58.491 53 21 1 51 103 146 197 1.21e-11 65.1
Msa0277950 AT3G46640.3 58.491 53 21 1 51 103 146 197 1.24e-11 65.1
Msa0277950 AT2G20570.1 51.471 68 31 2 35 102 140 205 1.35e-11 65.5
Msa0277950 AT2G03500.1 53.226 62 29 0 47 108 231 292 1.63e-11 65.5
Msa0277950 AT2G01760.3 45.333 75 38 2 49 123 135 206 1.66e-11 64.7
Msa0277950 AT5G44190.1 46.154 65 34 1 38 102 136 199 2.79e-11 64.3
Msa0277950 AT2G01760.1 45.333 75 38 2 49 123 200 271 2.96e-11 64.3
Msa0277950 AT5G44190.2 46.154 65 34 1 38 102 136 199 3.20e-11 63.9
Msa0277950 AT2G01760.2 45.333 75 38 2 49 123 228 299 4.22e-11 63.9
Msa0277950 AT2G40970.1 56.364 55 23 1 49 103 105 158 4.60e-11 62.8
Msa0277950 AT3G25790.2 58.696 46 19 0 54 99 201 246 9.79e-11 62.0

Find 99 sgRNAs with CRISPR-Local

Find 304 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCCTGATTCGAGTTTAAT+TGG 0.223090 2_3:-10840087 Msa0277950:CDS
CTCTAAAAGTAATTAAATTA+AGG 0.260834 2_3:+10837990 None:intergenic
GATCACTTTGATCCTTCTTT+AGG 0.297515 2_3:-10838199 Msa0277950:CDS
TTTAGAGAACTGATTTGAAT+TGG 0.300478 2_3:-10837974 Msa0277950:three_prime_UTR
CCATGTTAAGAGCCATTTGC+AGG 0.349218 2_3:-10839645 Msa0277950:intron
TACAAGAAGTGGTGAGGAAT+TGG 0.349818 2_3:-10838170 Msa0277950:CDS
TGCAGAGACAGCTACAATTA+AGG 0.349960 2_3:-10838642 Msa0277950:CDS
TGGATTTCATAGATCTCATC+TGG 0.363640 2_3:-10842252 Msa0277950:five_prime_UTR
CTTCCAATTAAACTCGAATC+AGG 0.368180 2_3:+10840084 None:intergenic
TTAGTTGATCTCTCGCCATT+AGG 0.374486 2_3:+10838340 None:intergenic
CATAACTCTGAGGACACCTT+TGG 0.375653 2_3:+10839691 None:intergenic
CAAAGGTGTCCTCAGAGTTA+TGG 0.377183 2_3:-10839690 Msa0277950:CDS
ATGTGCTGACCATGAACTAA+TGG 0.385679 2_3:+10840057 None:intergenic
GAACCGATGACACCAGATTC+TGG 0.386395 2_3:-10838391 Msa0277950:CDS
AAGACTGACAAGAAAGAAAC+GGG 0.390608 2_3:-10839347 Msa0277950:CDS
TGAGTTGAGATTAGAGTTGT+TGG 0.390955 2_3:+10839984 None:intergenic
GGAAAACAGCGGTTGGTTGC+TGG 0.394253 2_3:+10838230 None:intergenic
AAAGGTGTCCTCAGAGTTAT+GGG 0.402741 2_3:-10839689 Msa0277950:CDS
ACAATTAAGGATAGAAGCTC+AGG 0.410929 2_3:-10838629 Msa0277950:CDS
GGAAGCAATCCATTAGTTCA+TGG 0.415844 2_3:-10840066 Msa0277950:CDS
TTAGAGAACTGATTTGAATT+GGG 0.421756 2_3:-10837973 Msa0277950:three_prime_UTR
CATTTGAAGCTTTAGTGCTT+CGG 0.424328 2_3:+10838968 None:intergenic
GATATGCTTTCCAATCTAGA+TGG 0.424452 2_3:-10839323 Msa0277950:CDS
TCCTGTTGGGACATATCTCC+TGG 0.428112 2_3:+10838517 None:intergenic
TTATATGCATTCTTAGTTGA+TGG 0.434188 2_3:-10838081 Msa0277950:three_prime_UTR
TCTGCTGAAAGACTCTTTGC+TGG 0.439579 2_3:+10838433 None:intergenic
GGTGCTTCCTCATCAGATAC+TGG 0.444138 2_3:-10838272 Msa0277950:CDS
CTTTCAGAAGCACCCGGCTC+TGG 0.447624 2_3:-10838556 Msa0277950:CDS
CCGACTTGGCAACCAGAATC+TGG 0.448662 2_3:+10838379 None:intergenic
CTCGAATCAGGAACATTAGT+TGG 0.450860 2_3:+10840096 None:intergenic
TCTCCTGCTGACAGTCCTAA+TGG 0.454643 2_3:-10838355 Msa0277950:CDS
TCACGATGAACCATCTAGAT+TGG 0.455940 2_3:+10839313 None:intergenic
GTTGATGCTGTGGCTCAACT+TGG 0.466308 2_3:-10839919 Msa0277950:CDS
AAGAAGGATCAAAGTGATCT+CGG 0.468691 2_3:+10838203 None:intergenic
GAAGCGACTACATGAACAAT+TGG 0.469698 2_3:-10838937 Msa0277950:CDS
CGAAGCACTAAAGCTTCAAA+TGG 0.471790 2_3:-10838967 Msa0277950:CDS
TCCAGGAGATATGTCCCAAC+AGG 0.476349 2_3:-10838518 Msa0277950:CDS
ATAACTCTGAGGACACCTTT+GGG 0.476739 2_3:+10839692 None:intergenic
TCTTATTGTCGAGCTCCTGT+TGG 0.481895 2_3:+10838503 None:intergenic
CTTATTGTCGAGCTCCTGTT+GGG 0.485642 2_3:+10838504 None:intergenic
CTCAGAGTTATGGGTGTACA+AGG 0.485843 2_3:-10839680 Msa0277950:CDS
GGTAGGAGCGCTATGGATCC+TGG 0.493466 2_3:-10840027 Msa0277950:CDS
CTACACAGCTCCGAAATTGA+AGG 0.498072 2_3:-10842299 Msa0277950:five_prime_UTR
CATGGTCAGCACATAGAGTC+TGG 0.500025 2_3:-10840048 Msa0277950:CDS
ATTAAATTAGTAGCCCAAGA+TGG 0.500210 2_3:+10837895 None:intergenic
GAGTGCACATTACCTGCAAA+TGG 0.500488 2_3:+10839633 None:intergenic
AAAGACTGACAAGAAAGAAA+CGG 0.503951 2_3:-10839348 Msa0277950:CDS
TCCTTAGGTGCCACACCCAA+AGG 0.508503 2_3:-10839707 Msa0277950:intron
GATGCTGTGGCTCAACTTGG+TGG 0.512420 2_3:-10839916 Msa0277950:CDS
GCTCGACAATAAGACTGAAC+CGG 0.517717 2_3:-10838494 Msa0277950:CDS
TGGTGCTGATACACCAGAGC+CGG 0.522258 2_3:+10838543 None:intergenic
AATCTGGTGTCATCGGTTCA+TGG 0.523061 2_3:+10838395 None:intergenic
GATCAAAGTGATCTCGGGTA+AGG 0.525714 2_3:+10838209 None:intergenic
CCTGCAAATGGCTCTTAACA+TGG 0.525821 2_3:+10839645 None:intergenic
CCAGATTCTGGTTGCCAAGT+CGG 0.528854 2_3:-10838379 Msa0277950:CDS
TTAAATTAGTAGCCCAAGAT+GGG 0.529038 2_3:+10837896 None:intergenic
GTACCATCGGATGAGCAGTC+AGG 0.531157 2_3:+10839513 None:intergenic
ACTCAAAGCAACGTTTGCGT+TGG 0.531602 2_3:-10839966 Msa0277950:CDS
AGAAGGATCAAAGTGATCTC+GGG 0.531953 2_3:+10838204 None:intergenic
GGTGTATCAGCACCAACTCC+AGG 0.533448 2_3:-10838535 Msa0277950:CDS
AGCGCTATGGATCCTGGTAG+TGG 0.534994 2_3:-10840021 Msa0277950:CDS
CTTGTAGACATGCCTAAAGA+AGG 0.541592 2_3:+10838187 None:intergenic
TTAGGCATGTCTACAAGAAG+TGG 0.546196 2_3:-10838181 Msa0277950:CDS
CGGGTAAGGAAAACAGCGGT+TGG 0.546421 2_3:+10838223 None:intergenic
GGTGCTGATACACCAGAGCC+GGG 0.552630 2_3:+10838544 None:intergenic
TCTGAGGACACCTTTGGGTG+TGG 0.554653 2_3:+10839697 None:intergenic
CAACCAGAATCTGGTGTCAT+CGG 0.558599 2_3:+10838388 None:intergenic
AGAGTCTGGTAGGAGCGCTA+TGG 0.562642 2_3:-10840034 Msa0277950:CDS
GCGGTTGGTTGCTGGTATGA+TGG 0.564870 2_3:+10838238 None:intergenic
TGTCAGCAGGAGAACCGACT+TGG 0.567410 2_3:+10838365 None:intergenic
AATTGGATAAGCTTGGTGGC+AGG 0.568213 2_3:-10838153 Msa0277950:CDS
TGGGACATATCTCCTGGAGT+TGG 0.568536 2_3:+10838523 None:intergenic
AGACTGTTTCCTCCACTACC+AGG 0.568775 2_3:+10840009 None:intergenic
AAGAGCAACAGCGACTCAGT+GGG 0.575024 2_3:-10838582 Msa0277950:CDS
TCGCCATTAGGACTGTCAGC+AGG 0.575904 2_3:+10838352 None:intergenic
TACACAGCTCCGAAATTGAA+GGG 0.581590 2_3:-10842298 Msa0277950:five_prime_UTR
TATGCATTCTTAGTTGATGG+AGG 0.586098 2_3:-10838078 Msa0277950:three_prime_UTR
GGTGAGGAATTGGATAAGCT+TGG 0.592014 2_3:-10838160 Msa0277950:CDS
TTACCTGACTGCTCATCCGA+TGG 0.595863 2_3:-10839516 Msa0277950:intron
GCGACTACATGAACAATTGG+AGG 0.598747 2_3:-10838934 Msa0277950:intron
TGAACGCTTTGTTGATGCTG+TGG 0.601507 2_3:-10839929 Msa0277950:CDS
GGGGTTCTTTCAGAAGCACC+CGG 0.619430 2_3:-10838562 Msa0277950:CDS
GGATTTCATAGATCTCATCT+GGG 0.628659 2_3:-10842251 Msa0277950:intron
GTCAGCACATAGAGTCTGGT+AGG 0.630464 2_3:-10840044 Msa0277950:CDS
AGCACTAAAGCTTCAAATGG+AGG 0.636135 2_3:-10838964 Msa0277950:CDS
GAAGAGCAACAGCGACTCAG+TGG 0.642615 2_3:-10838583 Msa0277950:CDS
CTTGACTCCAGTATCTGATG+AGG 0.648174 2_3:+10838265 None:intergenic
CATGTCTACAAGAAGTGGTG+AGG 0.650625 2_3:-10838176 Msa0277950:CDS
GAGGAATTGGATAAGCTTGG+TGG 0.651592 2_3:-10838157 Msa0277950:CDS
CAATTAAGGATAGAAGCTCA+GGG 0.654933 2_3:-10838628 Msa0277950:CDS
GGGGAAAATACTCACGATCG+GGG 0.658151 2_3:+10839893 None:intergenic
GCTATGGATCCTGGTAGTGG+AGG 0.662575 2_3:-10840018 Msa0277950:CDS
ACACAGCTCCGAAATTGAAG+GGG 0.667923 2_3:-10842297 Msa0277950:five_prime_UTR
GACTGACAAGAAAGAAACGG+GGG 0.678708 2_3:-10839345 Msa0277950:CDS
AGCGTATTTGAAATCAGACA+TGG 0.689566 2_3:-10838293 Msa0277950:CDS
AGAGCAACAGCGACTCAGTG+GGG 0.711050 2_3:-10838581 Msa0277950:CDS
CTTGTACACCCATAACTCTG+AGG 0.715783 2_3:+10839681 None:intergenic
ATCTCGGGTAAGGAAAACAG+CGG 0.741797 2_3:+10838219 None:intergenic
AGACTGACAAGAAAGAAACG+GGG 0.753105 2_3:-10839346 Msa0277950:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAAATAAATAAATGTTAAT+TGG - chr2_3:10838448-10838467 Msa0277950:CDS 10.0%
!!! TTTTATTAATTTCAATCAAA+TGG - chr2_3:10839813-10839832 Msa0277950:intron 10.0%
!!! TTGTATTGTTTTTACATTTT+CGG - chr2_3:10838155-10838174 Msa0277950:CDS 15.0%
!! ATACAATATTAACAACAATT+AGG + chr2_3:10838423-10838442 None:intergenic 15.0%
!! ATAAATAAATGTTAATTGGT+TGG - chr2_3:10838452-10838471 Msa0277950:CDS 15.0%
!! TTAAATAAATGTTAATTGGT+TGG - chr2_3:10838650-10838669 Msa0277950:intron 15.0%
!! ATTAAATTTATGATCTATTG+TGG - chr2_3:10839624-10839643 Msa0277950:intron 15.0%
!!! GATTTAAAAACTTTTAGATA+AGG + chr2_3:10840531-10840550 None:intergenic 15.0%
!! CATTAAAAAAACAAGAATAA+TGG + chr2_3:10840685-10840704 None:intergenic 15.0%
!!! TAAAAAGTTATGTTTCTTTT+AGG - chr2_3:10841144-10841163 Msa0277950:intron 15.0%
!!! TTTTATTTTGAATCAAGTAA+AGG - chr2_3:10841532-10841551 Msa0277950:intron 15.0%
!!! CTCTAAAAGTAATTAAATTA+AGG + chr2_3:10842284-10842303 None:intergenic 15.0%
!!! AAAGCATTCATTGATTTTTA+GGG - chr2_3:10838550-10838569 Msa0277950:CDS 20.0%
!! ATACAACATTAACAACAATT+AGG + chr2_3:10838619-10838638 None:intergenic 20.0%
!!! TTTTGTAAGGATTGATTTTT+AGG - chr2_3:10838743-10838762 Msa0277950:intron 20.0%
!!! TTTGTAAGGATTGATTTTTA+GGG - chr2_3:10838744-10838763 Msa0277950:intron 20.0%
!! ATCACTAAGATAACATATAT+AGG + chr2_3:10838872-10838891 None:intergenic 20.0%
!! TAGCAAAGAATCTATATAAT+AGG + chr2_3:10839148-10839167 None:intergenic 20.0%
!!! CTTTTTTTAATGACAAATGT+TGG - chr2_3:10839330-10839349 Msa0277950:CDS 20.0%
!!! ACAGTTTGAAAAGTTTTTTA+TGG - chr2_3:10839919-10839938 Msa0277950:CDS 20.0%
!! AAATCAGTAAGATCTAAATA+TGG + chr2_3:10840807-10840826 None:intergenic 20.0%
!! AGAATGCTTAATACTTTAAA+CGG + chr2_3:10840842-10840861 None:intergenic 20.0%
!! AAAGTTATCAGCAAATTTAA+AGG + chr2_3:10840881-10840900 None:intergenic 20.0%
!!! ACTTTTTAATTGTCTTATAG+AGG - chr2_3:10840896-10840915 Msa0277950:intron 20.0%
!!! CTTTTTAATTGTCTTATAGA+GGG - chr2_3:10840897-10840916 Msa0277950:intron 20.0%
!!! TTTTTAATTGTCTTATAGAG+GGG - chr2_3:10840898-10840917 Msa0277950:intron 20.0%
!! TATCATAATTATACAAGGAA+TGG - chr2_3:10841008-10841027 Msa0277950:intron 20.0%
!! TAGAAAAAATAACAGTTACA+AGG + chr2_3:10841598-10841617 None:intergenic 20.0%
!!! TTATTTTTTCTATGTGAAAC+AGG - chr2_3:10841606-10841625 Msa0277950:intron 20.0%
!! GAAAATTTTGTATACGTAGT+TGG - chr2_3:10837999-10838018 Msa0277950:three_prime_UTR 25.0%
! GAAGAGAATGAAAATTGAAA+AGG + chr2_3:10838077-10838096 None:intergenic 25.0%
! GAGAAAGTAAGGAATAAAAA+GGG + chr2_3:10838113-10838132 None:intergenic 25.0%
! AGAGAAAGTAAGGAATAAAA+AGG + chr2_3:10838114-10838133 None:intergenic 25.0%
! TAAAACCAAATAGGCAAAAA+GGG + chr2_3:10838184-10838203 None:intergenic 25.0%
!!! TTTTTAGCTTTTGTTGATGT+GGG - chr2_3:10838358-10838377 Msa0277950:CDS 25.0%
! AATCAATGAATGCTTTCAAA+AGG + chr2_3:10838546-10838565 None:intergenic 25.0%
!!! GAAAGCATTCATTGATTTTT+AGG - chr2_3:10838549-10838568 Msa0277950:CDS 25.0%
!! TAGGGTTTTACTTGATATTT+AGG - chr2_3:10838568-10838587 Msa0277950:CDS 25.0%
!! AGGGTTTTACTTGATATTTA+GGG - chr2_3:10838569-10838588 Msa0277950:CDS 25.0%
! GTTGTAAATGAGATGAAAAA+TGG - chr2_3:10838785-10838804 Msa0277950:intron 25.0%
! TGTTATCTTAGTGATTAATC+AGG - chr2_3:10838877-10838896 Msa0277950:intron 25.0%
!!! ATTTTCAACTCCACTTTTTA+GGG - chr2_3:10839024-10839043 Msa0277950:intron 25.0%
! ATTTAGTTATAGCAGTGATT+TGG - chr2_3:10839106-10839125 Msa0277950:intron 25.0%
! AAATTGAATTCAACTTTCTG+TGG - chr2_3:10839227-10839246 Msa0277950:intron 25.0%
!! TAGTTTAAGTTTCTTAGACA+TGG - chr2_3:10839505-10839524 Msa0277950:intron 25.0%
!! GTGTTTGTTAAATACTGTAT+TGG - chr2_3:10839854-10839873 Msa0277950:intron 25.0%
! CGTTGAAAAAAATTGTACAA+CGG + chr2_3:10839899-10839918 None:intergenic 25.0%
! AACTGAATCTGATTAAGAAT+TGG - chr2_3:10839982-10840001 Msa0277950:CDS 25.0%
! ATTTCAGCTAACGAAAAAAT+GGG + chr2_3:10840401-10840420 None:intergenic 25.0%
! TTGGTTACATCATACATATT+TGG - chr2_3:10840434-10840453 Msa0277950:intron 25.0%
!! CAGATATCAAATACTTTTGT+TGG - chr2_3:10840459-10840478 Msa0277950:intron 25.0%
! TAAATTCATCATCTAGCATT+AGG + chr2_3:10840493-10840512 None:intergenic 25.0%
! TGGGTTATCATAATTATACA+AGG - chr2_3:10841003-10841022 Msa0277950:intron 25.0%
!!! TATAGATTTTTTTCCACCAA+CGG + chr2_3:10841186-10841205 None:intergenic 25.0%
! TTATATGCATTCTTAGTTGA+TGG - chr2_3:10842190-10842209 Msa0277950:intron 25.0%
!!! TTTAGAGAACTGATTTGAAT+TGG - chr2_3:10842297-10842316 Msa0277950:five_prime_UTR 25.0%
!!! TTAGAGAACTGATTTGAATT+GGG - chr2_3:10842298-10842317 Msa0277950:five_prime_UTR 25.0%
! AAAAACTCAACCTTGAAAAT+GGG + chr2_3:10842352-10842371 None:intergenic 25.0%
!!! ATTTTCAAGGTTGAGTTTTT+GGG - chr2_3:10842352-10842371 Msa0277950:five_prime_UTR 25.0%
TGAAAGAAAGAGAGAAAGTA+AGG + chr2_3:10838124-10838143 None:intergenic 30.0%
CTAAAACCAAATAGGCAAAA+AGG + chr2_3:10838185-10838204 None:intergenic 30.0%
!!! CTTTTTAGCTTTTGTTGATG+TGG - chr2_3:10838357-10838376 Msa0277950:CDS 30.0%
!!! TTTTAGCTTTTGTTGATGTG+GGG - chr2_3:10838359-10838378 Msa0277950:CDS 30.0%
GCTTTCAAAAGGTTAAAACA+TGG + chr2_3:10838535-10838554 None:intergenic 30.0%
!!! ATTTAGGGTTTTTTCTGTTG+TGG - chr2_3:10838584-10838603 Msa0277950:CDS 30.0%
!!! TTTAGGGTTTTTTCTGTTGT+GGG - chr2_3:10838585-10838604 Msa0277950:CDS 30.0%
CCTTACAAAAACTTCAAACA+TGG + chr2_3:10838733-10838752 None:intergenic 30.0%
!! CCATGTTTGAAGTTTTTGTA+AGG - chr2_3:10838730-10838749 Msa0277950:intron 30.0%
ATAGCTACCATAAGTGAATT+GGG + chr2_3:10838828-10838847 None:intergenic 30.0%
TATAGCTACCATAAGTGAAT+TGG + chr2_3:10838829-10838848 None:intergenic 30.0%
TTCACTTATGGTAGCTATAA+AGG - chr2_3:10838830-10838849 Msa0277950:intron 30.0%
!!! GATTTTCAACTCCACTTTTT+AGG - chr2_3:10839023-10839042 Msa0277950:intron 30.0%
!! AGTGATTTGGTGATATTTGA+AGG - chr2_3:10839119-10839138 Msa0277950:intron 30.0%
!! GTGATTTGGTGATATTTGAA+GGG - chr2_3:10839120-10839139 Msa0277950:intron 30.0%
!! TGATTTGGTGATATTTGAAG+GGG - chr2_3:10839121-10839140 Msa0277950:intron 30.0%
TATCTAGAGATAGATGATGA+TGG + chr2_3:10839205-10839224 None:intergenic 30.0%
!!! TTGATTTTTTCTCCTTTGTC+CGG - chr2_3:10839368-10839387 Msa0277950:intron 30.0%
ACTTGGTAAACTTCTTGTAT+GGG + chr2_3:10839453-10839472 None:intergenic 30.0%
AACTTGGTAAACTTCTTGTA+TGG + chr2_3:10839454-10839473 None:intergenic 30.0%
TTCTAAGTCAAACTAGAACT+TGG + chr2_3:10839470-10839489 None:intergenic 30.0%
!! AGTTCTAGTTTGACTTAGAA+TGG - chr2_3:10839470-10839489 Msa0277950:intron 30.0%
AAACTATTATTAGTGAACCC+GGG + chr2_3:10839738-10839757 None:intergenic 30.0%
CAAACTATTATTAGTGAACC+CGG + chr2_3:10839739-10839758 None:intergenic 30.0%
ATTTCAATCAAATGGAGCAT+TGG - chr2_3:10839821-10839840 Msa0277950:intron 30.0%
AAATTGTACAACGGATCATT+TGG + chr2_3:10839890-10839909 None:intergenic 30.0%
AACTCTCTTTCTTTCAATCA+TGG + chr2_3:10839961-10839980 None:intergenic 30.0%
ATTGGAATTAGAACATGTTG+AGG - chr2_3:10840000-10840019 Msa0277950:CDS 30.0%
TTGGAATTAGAACATGTTGA+GGG - chr2_3:10840001-10840020 Msa0277950:CDS 30.0%
AGACAGAAACACACAAAAAT+TGG + chr2_3:10840028-10840047 None:intergenic 30.0%
!! AAAGGTTGGTAAAGAAATGA+GGG + chr2_3:10840070-10840089 None:intergenic 30.0%
!! AAAAGGTTGGTAAAGAAATG+AGG + chr2_3:10840071-10840090 None:intergenic 30.0%
ACTTAATTGCAGATCGTAAA+AGG + chr2_3:10840088-10840107 None:intergenic 30.0%
TTTCAGCTAACGAAAAAATG+GGG + chr2_3:10840400-10840419 None:intergenic 30.0%
CATTTCAGCTAACGAAAAAA+TGG + chr2_3:10840402-10840421 None:intergenic 30.0%
TGAAATGTAATGCGTAGAAT+TGG - chr2_3:10840415-10840434 Msa0277950:intron 30.0%
TGCTAGATGATGAATTTATC+AGG - chr2_3:10840495-10840514 Msa0277950:intron 30.0%
! TCTATACTATCCTTTTCCTT+AGG - chr2_3:10840549-10840568 Msa0277950:intron 30.0%
!! ATAATGGATACCACATTAAG+AGG + chr2_3:10840669-10840688 None:intergenic 30.0%
ATTTAAAGGAGGAAACAAAG+TGG + chr2_3:10840867-10840886 None:intergenic 30.0%
GTTATCAGCAAATTTAAAGG+AGG + chr2_3:10840878-10840897 None:intergenic 30.0%
AAAGACTGACAAGAAAGAAA+CGG - chr2_3:10840923-10840942 Msa0277950:intron 30.0%
TGATAACCCAAAAGCAAAAA+GGG + chr2_3:10840993-10841012 None:intergenic 30.0%
ATGATAACCCAAAAGCAAAA+AGG + chr2_3:10840994-10841013 None:intergenic 30.0%
CTAAATAGAACCATCTCATT+TGG - chr2_3:10841080-10841099 Msa0277950:intron 30.0%
TAATAATGTTACATGTCTGC+AGG - chr2_3:10841267-10841286 Msa0277950:intron 30.0%
AATAATGTTACATGTCTGCA+GGG - chr2_3:10841268-10841287 Msa0277950:intron 30.0%
GTGAGATGAAATGATGATAT+TGG - chr2_3:10841447-10841466 Msa0277950:intron 30.0%
AAGGAATAATAGACAGAATG+AGG - chr2_3:10841551-10841570 Msa0277950:intron 30.0%
AGGAATAATAGACAGAATGA+GGG - chr2_3:10841552-10841571 Msa0277950:intron 30.0%
CAAAAACTCAACCTTGAAAA+TGG + chr2_3:10842353-10842372 None:intergenic 30.0%
!! CATTTTCAAGGTTGAGTTTT+TGG - chr2_3:10842351-10842370 Msa0277950:five_prime_UTR 30.0%
! ATTTTCGTCCCCTTCAATTT+CGG + chr2_3:10837985-10838004 None:intergenic 35.0%
TGGATTTCATAGATCTCATC+TGG - chr2_3:10838019-10838038 Msa0277950:three_prime_UTR 35.0%
GGATTTCATAGATCTCATCT+GGG - chr2_3:10838020-10838039 Msa0277950:three_prime_UTR 35.0%
AGCTGAAGCTAAAACCAAAT+AGG + chr2_3:10838193-10838212 None:intergenic 35.0%
AGCACAAAACTACACAAGAA+GGG + chr2_3:10838287-10838306 None:intergenic 35.0%
!!! TTTAGCTTTTGTTGATGTGG+GGG - chr2_3:10838360-10838379 Msa0277950:CDS 35.0%
AAAACCCATAACACTCATCA+TGG + chr2_3:10838507-10838526 None:intergenic 35.0%
TTCAGACATGATGAGTCATA+TGG - chr2_3:10838695-10838714 Msa0277950:intron 35.0%
TCAGACATGATGAGTCATAT+GGG - chr2_3:10838696-10838715 Msa0277950:intron 35.0%
TCTGTTTCCCAATTCACTTA+TGG - chr2_3:10838818-10838837 Msa0277950:intron 35.0%
ACTTATGGTAGCTATAAAGG+TGG - chr2_3:10838833-10838852 Msa0277950:intron 35.0%
!!! AACTCCACTTTTTAGGGTTT+AGG - chr2_3:10839030-10839049 Msa0277950:intron 35.0%
!!! ACTCCACTTTTTAGGGTTTA+GGG - chr2_3:10839031-10839050 Msa0277950:intron 35.0%
ATGAAAGGTTACCTATGCTA+GGG - chr2_3:10839057-10839076 Msa0277950:intron 35.0%
!! GATTTGGTGATATTTGAAGG+GGG - chr2_3:10839122-10839141 Msa0277950:intron 35.0%
!!! TGATTTTTTCTCCTTTGTCC+GGG - chr2_3:10839369-10839388 Msa0277950:intron 35.0%
!! TCAATTGGTTTGACCTAAGA+AGG + chr2_3:10839408-10839427 None:intergenic 35.0%
TTTCCTAGAGATACAAACGA+AGG - chr2_3:10839560-10839579 Msa0277950:intron 35.0%
!!! AAATGGAGCATTGGTTTTCT+GGG - chr2_3:10839830-10839849 Msa0277950:intron 35.0%
TGGAATTAGAACATGTTGAG+GGG - chr2_3:10840002-10840021 Msa0277950:CDS 35.0%
!! AAGGTTGGTAAAGAAATGAG+GGG + chr2_3:10840069-10840088 None:intergenic 35.0%
AATTGCAGATCGTAAAAGGT+TGG + chr2_3:10840084-10840103 None:intergenic 35.0%
! GTTCCTGATTCGAGTTTAAT+TGG - chr2_3:10840184-10840203 Msa0277950:intron 35.0%
CTTCCAATTAAACTCGAATC+AGG + chr2_3:10840190-10840209 None:intergenic 35.0%
! TGAGTTGAGATTAGAGTTGT+TGG + chr2_3:10840290-10840309 None:intergenic 35.0%
! CAGGTAAGTATTTTGCTAGT+CGG + chr2_3:10840742-10840761 None:intergenic 35.0%
AAATCAGATTGACGTACCAT+CGG + chr2_3:10840774-10840793 None:intergenic 35.0%
AAGACTGACAAGAAAGAAAC+GGG - chr2_3:10840924-10840943 Msa0277950:intron 35.0%
GATATGCTTTCCAATCTAGA+TGG - chr2_3:10840948-10840967 Msa0277950:intron 35.0%
! ATAGCTTCCCTTTTTGCTTT+TGG - chr2_3:10840983-10841002 Msa0277950:intron 35.0%
!! TAGCTTCCCTTTTTGCTTTT+GGG - chr2_3:10840984-10841003 Msa0277950:intron 35.0%
! GAGATGGTTCTATTTAGTTC+AGG + chr2_3:10841077-10841096 None:intergenic 35.0%
GGATGTAAAACCAAATGAGA+TGG + chr2_3:10841093-10841112 None:intergenic 35.0%
!!! TCTCATTTGGTTTTACATCC+TGG - chr2_3:10841093-10841112 Msa0277950:intron 35.0%
ATAATGTTACATGTCTGCAG+GGG - chr2_3:10841269-10841288 Msa0277950:intron 35.0%
! CATTTGAAGCTTTAGTGCTT+CGG + chr2_3:10841306-10841325 None:intergenic 35.0%
ACAATTAAGGATAGAAGCTC+AGG - chr2_3:10841642-10841661 Msa0277950:intron 35.0%
CAATTAAGGATAGAAGCTCA+GGG - chr2_3:10841643-10841662 Msa0277950:intron 35.0%
! AGCGTATTTGAAATCAGACA+TGG - chr2_3:10841978-10841997 Msa0277950:intron 35.0%
AAGAAGGATCAAAGTGATCT+CGG + chr2_3:10842071-10842090 None:intergenic 35.0%
GATCACTTTGATCCTTCTTT+AGG - chr2_3:10842072-10842091 Msa0277950:intron 35.0%
! TGTGATTTTGTGTAATCTGC+AGG - chr2_3:10842145-10842164 Msa0277950:intron 35.0%
! GTGATTTTGTGTAATCTGCA+GGG - chr2_3:10842146-10842165 Msa0277950:intron 35.0%
!! TATGCATTCTTAGTTGATGG+AGG - chr2_3:10842193-10842212 Msa0277950:intron 35.0%
! GTCATATGTTCCCATTTTCA+AGG - chr2_3:10842339-10842358 Msa0277950:five_prime_UTR 35.0%
TACACAGCTCCGAAATTGAA+GGG - chr2_3:10837973-10837992 Msa0277950:three_prime_UTR 40.0%
GAGCACAAAACTACACAAGA+AGG + chr2_3:10838288-10838307 None:intergenic 40.0%
!!! TGTAGTTTTGTGCTCTGTGT+TGG - chr2_3:10838293-10838312 Msa0277950:CDS 40.0%
!!! TTGCTGGGTTTTTTCTGTTG+TGG - chr2_3:10838388-10838407 Msa0277950:CDS 40.0%
!!! TGCTGGGTTTTTTCTGTTGT+GGG - chr2_3:10838389-10838408 Msa0277950:CDS 40.0%
TTCAGCCATGATGAGTGTTA+TGG - chr2_3:10838499-10838518 Msa0277950:CDS 40.0%
TCAGCCATGATGAGTGTTAT+GGG - chr2_3:10838500-10838519 Msa0277950:CDS 40.0%
!!! CTCCACTTTTTAGGGTTTAG+GGG - chr2_3:10839032-10839051 Msa0277950:intron 40.0%
!!! TCCACTTTTTAGGGTTTAGG+GGG - chr2_3:10839033-10839052 Msa0277950:intron 40.0%
GATGAAAGGTTACCTATGCT+AGG - chr2_3:10839056-10839075 Msa0277950:intron 40.0%
TAACATCCTTTCCCTAGCAT+AGG + chr2_3:10839071-10839090 None:intergenic 40.0%
TTTGAACCCAAGACCTTCTT+AGG - chr2_3:10839392-10839411 Msa0277950:intron 40.0%
GTTTGACCTAAGAAGGTCTT+GGG + chr2_3:10839401-10839420 None:intergenic 40.0%
AAACTTCTTGTATGGGTGGA+TGG + chr2_3:10839446-10839465 None:intergenic 40.0%
TGGTAAACTTCTTGTATGGG+TGG + chr2_3:10839450-10839469 None:intergenic 40.0%
AAAGTCAAGCCTCATGGAAT+AGG + chr2_3:10839541-10839560 None:intergenic 40.0%
TAGGAAAAAGTCAAGCCTCA+TGG + chr2_3:10839547-10839566 None:intergenic 40.0%
CATCCTTCGTTTGTATCTCT+AGG + chr2_3:10839566-10839585 None:intergenic 40.0%
!!! CAAATGGAGCATTGGTTTTC+TGG - chr2_3:10839829-10839848 Msa0277950:intron 40.0%
!!! AATGGAGCATTGGTTTTCTG+GGG - chr2_3:10839831-10839850 Msa0277950:intron 40.0%
CTCGAATCAGGAACATTAGT+TGG + chr2_3:10840178-10840197 None:intergenic 40.0%
GGAAGCAATCCATTAGTTCA+TGG - chr2_3:10840205-10840224 Msa0277950:intron 40.0%
! ATGTGCTGACCATGAACTAA+TGG + chr2_3:10840217-10840236 None:intergenic 40.0%
ATGGGGAAAATACTCACGAT+CGG + chr2_3:10840383-10840402 None:intergenic 40.0%
! ATAACTCTGAGGACACCTTT+GGG + chr2_3:10840582-10840601 None:intergenic 40.0%
!! AAAGGTGTCCTCAGAGTTAT+GGG - chr2_3:10840582-10840601 Msa0277950:intron 40.0%
! TGCTAGTCGGTATTTCTGTA+TGG + chr2_3:10840729-10840748 None:intergenic 40.0%
AGACTGACAAGAAAGAAACG+GGG - chr2_3:10840925-10840944 Msa0277950:intron 40.0%
TCACGATGAACCATCTAGAT+TGG + chr2_3:10840961-10840980 None:intergenic 40.0%
ACTAGTGCAAATTTGACTCC+AGG + chr2_3:10841114-10841133 None:intergenic 40.0%
!!! TTTTTTCCACCAACGGTTTG+AGG + chr2_3:10841179-10841198 None:intergenic 40.0%
TAATGTTACATGTCTGCAGG+GGG - chr2_3:10841270-10841289 Msa0277950:intron 40.0%
! CGAAGCACTAAAGCTTCAAA+TGG - chr2_3:10841304-10841323 Msa0277950:intron 40.0%
AGCACTAAAGCTTCAAATGG+AGG - chr2_3:10841307-10841326 Msa0277950:intron 40.0%
GAAGCGACTACATGAACAAT+TGG - chr2_3:10841334-10841353 Msa0277950:intron 40.0%
CAGATCAGATCAGTTCAACA+AGG + chr2_3:10841370-10841389 None:intergenic 40.0%
TGCAGAGACAGCTACAATTA+AGG - chr2_3:10841629-10841648 Msa0277950:intron 40.0%
!! GCTCTTTAGCAGCTTTTTCT+GGG + chr2_3:10841813-10841832 None:intergenic 40.0%
!! TGCTCTTTAGCAGCTTTTTC+TGG + chr2_3:10841814-10841833 None:intergenic 40.0%
TTAGTTGATCTCTCGCCATT+AGG + chr2_3:10841934-10841953 None:intergenic 40.0%
TAAAAAGCAACGAGTAAGCG+TGG - chr2_3:10841951-10841970 Msa0277950:intron 40.0%
AGAAGGATCAAAGTGATCTC+GGG + chr2_3:10842070-10842089 None:intergenic 40.0%
CTTGTAGACATGCCTAAAGA+AGG + chr2_3:10842087-10842106 None:intergenic 40.0%
TTAGGCATGTCTACAAGAAG+TGG - chr2_3:10842090-10842109 Msa0277950:intron 40.0%
TACAAGAAGTGGTGAGGAAT+TGG - chr2_3:10842101-10842120 Msa0277950:intron 40.0%
CTACACAGCTCCGAAATTGA+AGG - chr2_3:10837972-10837991 Msa0277950:three_prime_UTR 45.0%
ACACAGCTCCGAAATTGAAG+GGG - chr2_3:10837974-10837993 Msa0277950:three_prime_UTR 45.0%
!!! CCACTTTTTAGGGTTTAGGG+GGG - chr2_3:10839034-10839053 Msa0277950:intron 45.0%
AGGTTACCTATGCTAGGGAA+AGG - chr2_3:10839062-10839081 Msa0277950:intron 45.0%
GCTAGCAGCATCAAGGATTT+AGG + chr2_3:10839178-10839197 None:intergenic 45.0%
GGTTTGACCTAAGAAGGTCT+TGG + chr2_3:10839402-10839421 None:intergenic 45.0%
TCTTGTATGGGTGGATGGAT+GGG + chr2_3:10839441-10839460 None:intergenic 45.0%
TTCTTGTATGGGTGGATGGA+TGG + chr2_3:10839442-10839461 None:intergenic 45.0%
AACAGTGTGCCTATTCCATG+AGG - chr2_3:10839529-10839548 Msa0277950:CDS 45.0%
! TTTAGACCCATGCTTTTGCC+CGG - chr2_3:10839717-10839736 Msa0277950:intron 45.0%
TGCTGTGTGCAGAAAGTCAT+TGG - chr2_3:10839783-10839802 Msa0277950:intron 45.0%
!!! ATGGAGCATTGGTTTTCTGG+GGG - chr2_3:10839832-10839851 Msa0277950:intron 45.0%
ACTCAAAGCAACGTTTGCGT+TGG - chr2_3:10840305-10840324 Msa0277950:intron 45.0%
! TGAACGCTTTGTTGATGCTG+TGG - chr2_3:10840342-10840361 Msa0277950:intron 45.0%
TGGGGAAAATACTCACGATC+GGG + chr2_3:10840382-10840401 None:intergenic 45.0%
! CATAACTCTGAGGACACCTT+TGG + chr2_3:10840583-10840602 None:intergenic 45.0%
!! CAAAGGTGTCCTCAGAGTTA+TGG - chr2_3:10840581-10840600 Msa0277950:intron 45.0%
CTTGTACACCCATAACTCTG+AGG + chr2_3:10840593-10840612 None:intergenic 45.0%
CTCAGAGTTATGGGTGTACA+AGG - chr2_3:10840591-10840610 Msa0277950:intron 45.0%
CCTGCAAATGGCTCTTAACA+TGG + chr2_3:10840629-10840648 None:intergenic 45.0%
CCATGTTAAGAGCCATTTGC+AGG - chr2_3:10840626-10840645 Msa0277950:intron 45.0%
GAGTGCACATTACCTGCAAA+TGG + chr2_3:10840641-10840660 None:intergenic 45.0%
TTGCAGGTAATGTGCACTCT+AGG - chr2_3:10840642-10840661 Msa0277950:intron 45.0%
CACTCTAGGTCCTCTTAATG+TGG - chr2_3:10840656-10840675 Msa0277950:intron 45.0%
GACTGACAAGAAAGAAACGG+GGG - chr2_3:10840926-10840945 Msa0277950:intron 45.0%
CATTCAATGCCTCAAACCGT+TGG - chr2_3:10841167-10841186 Msa0277950:intron 45.0%
GCGACTACATGAACAATTGG+AGG - chr2_3:10841337-10841356 Msa0277950:intron 45.0%
CTTATTGTCGAGCTCCTGTT+GGG + chr2_3:10841770-10841789 None:intergenic 45.0%
TCTTATTGTCGAGCTCCTGT+TGG + chr2_3:10841771-10841790 None:intergenic 45.0%
GCTCGACAATAAGACTGAAC+CGG - chr2_3:10841777-10841796 Msa0277950:intron 45.0%
!! TAGCAGCTTTTTCTGGGTCA+GGG + chr2_3:10841807-10841826 None:intergenic 45.0%
!! TTAGCAGCTTTTTCTGGGTC+AGG + chr2_3:10841808-10841827 None:intergenic 45.0%
TCTGCTGAAAGACTCTTTGC+TGG + chr2_3:10841841-10841860 None:intergenic 45.0%
!! AATCTGGTGTCATCGGTTCA+TGG + chr2_3:10841879-10841898 None:intergenic 45.0%
!! CAACCAGAATCTGGTGTCAT+CGG + chr2_3:10841886-10841905 None:intergenic 45.0%
CTTGACTCCAGTATCTGATG+AGG + chr2_3:10842009-10842028 None:intergenic 45.0%
ATCTCGGGTAAGGAAAACAG+CGG + chr2_3:10842055-10842074 None:intergenic 45.0%
GATCAAAGTGATCTCGGGTA+AGG + chr2_3:10842065-10842084 None:intergenic 45.0%
CATGTCTACAAGAAGTGGTG+AGG - chr2_3:10842095-10842114 Msa0277950:intron 45.0%
GGTGAGGAATTGGATAAGCT+TGG - chr2_3:10842111-10842130 Msa0277950:intron 45.0%
GAGGAATTGGATAAGCTTGG+TGG - chr2_3:10842114-10842133 Msa0277950:intron 45.0%
! AATTGGATAAGCTTGGTGGC+AGG - chr2_3:10842118-10842137 Msa0277950:intron 45.0%
!! TTGAGTTTTTGGGCCCATCT+TGG - chr2_3:10842362-10842381 Msa0277950:five_prime_UTR 45.0%
!! TGAGTTTTTGGGCCCATCTT+GGG - chr2_3:10842363-10842382 Msa0277950:five_prime_UTR 45.0%
!!! TTTTTTAAATAAATGTTAAT+TGG - chr2_3:10838646-10838665 Msa0277950:CDS 5.0%
! GGCGACCCTTTTTGCCTATT+TGG - chr2_3:10838176-10838195 Msa0277950:CDS 50.0%
! AGCTTTTGTTGATGTGGGGG+AGG - chr2_3:10838363-10838382 Msa0277950:CDS 50.0%
CCCCCCTAAACCCTAAAAAG+TGG + chr2_3:10839037-10839056 None:intergenic 50.0%
TAGGGTTTAGGGGGGATGAA+AGG - chr2_3:10839042-10839061 Msa0277950:intron 50.0%
!! TGGACTAGCTAGCAGCATCA+AGG + chr2_3:10839185-10839204 None:intergenic 50.0%
TTGGGTTCAAACCCGGACAA+AGG + chr2_3:10839383-10839402 None:intergenic 50.0%
GAAGGTCTTGGGTTCAAACC+CGG + chr2_3:10839390-10839409 None:intergenic 50.0%
ATGGGTGGGGGTAGATCAAT+TGG + chr2_3:10839423-10839442 None:intergenic 50.0%
! TTAGACCCATGCTTTTGCCC+GGG - chr2_3:10839718-10839737 Msa0277950:intron 50.0%
CATGGTCAGCACATAGAGTC+TGG - chr2_3:10840223-10840242 Msa0277950:intron 50.0%
GTCAGCACATAGAGTCTGGT+AGG - chr2_3:10840227-10840246 Msa0277950:intron 50.0%
AGACTGTTTCCTCCACTACC+AGG + chr2_3:10840265-10840284 None:intergenic 50.0%
GTTGATGCTGTGGCTCAACT+TGG - chr2_3:10840352-10840371 Msa0277950:intron 50.0%
GGGGAAAATACTCACGATCG+GGG + chr2_3:10840381-10840400 None:intergenic 50.0%
TTACCTGACTGCTCATCCGA+TGG - chr2_3:10840755-10840774 Msa0277950:intron 50.0%
TCAATGCCTCAAACCGTTGG+TGG - chr2_3:10841170-10841189 Msa0277950:intron 50.0%
AAGAGCAACAGCGACTCAGT+GGG - chr2_3:10841689-10841708 Msa0277950:intron 50.0%
TGGGACATATCTCCTGGAGT+TGG + chr2_3:10841751-10841770 None:intergenic 50.0%
TCCAGGAGATATGTCCCAAC+AGG - chr2_3:10841753-10841772 Msa0277950:intron 50.0%
TCCTGTTGGGACATATCTCC+TGG + chr2_3:10841757-10841776 None:intergenic 50.0%
!! AGCAGCTTTTTCTGGGTCAG+GGG + chr2_3:10841806-10841825 None:intergenic 50.0%
GAACCGATGACACCAGATTC+TGG - chr2_3:10841880-10841899 Msa0277950:intron 50.0%
! CCAGATTCTGGTTGCCAAGT+CGG - chr2_3:10841892-10841911 Msa0277950:intron 50.0%
TCTCCTGCTGACAGTCCTAA+TGG - chr2_3:10841916-10841935 Msa0277950:intron 50.0%
GGTGCTTCCTCATCAGATAC+TGG - chr2_3:10841999-10842018 Msa0277950:intron 50.0%
! TGATGTGGGGGAGGTTTTGC+TGG - chr2_3:10838372-10838391 Msa0277950:CDS 55.0%
! GATGTGGGGGAGGTTTTGCT+GGG - chr2_3:10838373-10838392 Msa0277950:CDS 55.0%
TATGGGTGGATGGATGGGTG+GGG + chr2_3:10839436-10839455 None:intergenic 55.0%
GTATGGGTGGATGGATGGGT+GGG + chr2_3:10839437-10839456 None:intergenic 55.0%
TGTATGGGTGGATGGATGGG+TGG + chr2_3:10839438-10839457 None:intergenic 55.0%
! GTGAACCCGGGCAAAAGCAT+GGG + chr2_3:10839726-10839745 None:intergenic 55.0%
AGTGAACCCGGGCAAAAGCA+TGG + chr2_3:10839727-10839746 None:intergenic 55.0%
GAGGGGTGACTCAAACATGC+AGG + chr2_3:10840052-10840071 None:intergenic 55.0%
!! AGAGTCTGGTAGGAGCGCTA+TGG - chr2_3:10840237-10840256 Msa0277950:intron 55.0%
AGCGCTATGGATCCTGGTAG+TGG - chr2_3:10840250-10840269 Msa0277950:intron 55.0%
!! GCTATGGATCCTGGTAGTGG+AGG - chr2_3:10840253-10840272 Msa0277950:intron 55.0%
GATGCTGTGGCTCAACTTGG+TGG - chr2_3:10840355-10840374 Msa0277950:intron 55.0%
GGGTGTGGCACCTAAGGAAA+AGG + chr2_3:10840562-10840581 None:intergenic 55.0%
! TCCTTAGGTGCCACACCCAA+AGG - chr2_3:10840564-10840583 Msa0277950:intron 55.0%
ACCTTTGGGTGTGGCACCTA+AGG + chr2_3:10840568-10840587 None:intergenic 55.0%
TCTGAGGACACCTTTGGGTG+TGG + chr2_3:10840577-10840596 None:intergenic 55.0%
GTACCATCGGATGAGCAGTC+AGG + chr2_3:10840761-10840780 None:intergenic 55.0%
GAAGAGCAACAGCGACTCAG+TGG - chr2_3:10841688-10841707 Msa0277950:intron 55.0%
AGAGCAACAGCGACTCAGTG+GGG - chr2_3:10841690-10841709 Msa0277950:intron 55.0%
!! GGGGTTCTTTCAGAAGCACC+CGG - chr2_3:10841709-10841728 Msa0277950:intron 55.0%
!! TGGTGCTGATACACCAGAGC+CGG + chr2_3:10841731-10841750 None:intergenic 55.0%
GGTGTATCAGCACCAACTCC+AGG - chr2_3:10841736-10841755 Msa0277950:intron 55.0%
! TTTTCTGGGTCAGGGGTTGC+CGG + chr2_3:10841799-10841818 None:intergenic 55.0%
!! CCGACTTGGCAACCAGAATC+TGG + chr2_3:10841895-10841914 None:intergenic 55.0%
TGTCAGCAGGAGAACCGACT+TGG + chr2_3:10841909-10841928 None:intergenic 55.0%
TCGCCATTAGGACTGTCAGC+AGG + chr2_3:10841922-10841941 None:intergenic 55.0%
! GCGGTTGGTTGCTGGTATGA+TGG + chr2_3:10842036-10842055 None:intergenic 55.0%
! GGAAAACAGCGGTTGGTTGC+TGG + chr2_3:10842044-10842063 None:intergenic 55.0%
CGGGTAAGGAAAACAGCGGT+TGG + chr2_3:10842051-10842070 None:intergenic 55.0%
ATGGGTGGATGGATGGGTGG+GGG + chr2_3:10839435-10839454 None:intergenic 60.0%
!! GGTAGGAGCGCTATGGATCC+TGG - chr2_3:10840244-10840263 Msa0277950:intron 60.0%
!! CTTTCAGAAGCACCCGGCTC+TGG - chr2_3:10841715-10841734 Msa0277950:intron 60.0%
!! GGTGCTGATACACCAGAGCC+GGG + chr2_3:10841730-10841749 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 10837902 10842391 10837902 ID=Msa0277950;Name=Msa0277950
chr2_3 mRNA 10837902 10842391 10837902 ID=Msa0277950-mRNA-1;Parent=Msa0277950;Name=Msa0277950-mRNA-1;_AED=0.06;_eAED=0.06;_QI=151|1|1|1|0.83|0.71|7|242|330
chr2_3 exon 10837902 10838665 10837902 ID=Msa0277950-mRNA-1:exon:4802;Parent=Msa0277950-mRNA-1
chr2_3 exon 10838935 10839004 10838935 ID=Msa0277950-mRNA-1:exon:4801;Parent=Msa0277950-mRNA-1
chr2_3 exon 10839317 10839377 10839317 ID=Msa0277950-mRNA-1:exon:4800;Parent=Msa0277950-mRNA-1
chr2_3 exon 10839517 10839559 10839517 ID=Msa0277950-mRNA-1:exon:4799;Parent=Msa0277950-mRNA-1
chr2_3 exon 10839646 10839722 10839646 ID=Msa0277950-mRNA-1:exon:4798;Parent=Msa0277950-mRNA-1
chr2_3 exon 10839908 10840138 10839908 ID=Msa0277950-mRNA-1:exon:4797;Parent=Msa0277950-mRNA-1
chr2_3 exon 10842252 10842391 10842252 ID=Msa0277950-mRNA-1:exon:4796;Parent=Msa0277950-mRNA-1
chr2_3 five_prime_UTR 10842252 10842391 10842252 ID=Msa0277950-mRNA-1:five_prime_utr;Parent=Msa0277950-mRNA-1
chr2_3 five_prime_UTR 10840128 10840138 10840128 ID=Msa0277950-mRNA-1:five_prime_utr;Parent=Msa0277950-mRNA-1
chr2_3 CDS 10839908 10840127 10839908 ID=Msa0277950-mRNA-1:cds;Parent=Msa0277950-mRNA-1
chr2_3 CDS 10839646 10839722 10839646 ID=Msa0277950-mRNA-1:cds;Parent=Msa0277950-mRNA-1
chr2_3 CDS 10839517 10839559 10839517 ID=Msa0277950-mRNA-1:cds;Parent=Msa0277950-mRNA-1
chr2_3 CDS 10839317 10839377 10839317 ID=Msa0277950-mRNA-1:cds;Parent=Msa0277950-mRNA-1
chr2_3 CDS 10838935 10839004 10838935 ID=Msa0277950-mRNA-1:cds;Parent=Msa0277950-mRNA-1
chr2_3 CDS 10838144 10838665 10838144 ID=Msa0277950-mRNA-1:cds;Parent=Msa0277950-mRNA-1
chr2_3 three_prime_UTR 10837902 10838143 10837902 ID=Msa0277950-mRNA-1:three_prime_utr;Parent=Msa0277950-mRNA-1
Gene Sequence

>Msa0277950

ATGTATCAGCCAACTAATGTTCCTGATTCGAGTTTAATTGGAAGCAATCCATTAGTTCATGGTCAGCACATAGAGTCTGGTAGGAGCGCTATGGATCCTGGTAGTGGAGGAAACAGTCTTGCCAACAACTCTAATCTCAACTCAAAGCAACGTTTGCGTTGGACACATGAGCTACATGAACGCTTTGTTGATGCTGTGGCTCAACTTGGTGGCCCCGATCGTGCCACACCCAAAGGTGTCCTCAGAGTTATGGGTGTACAAGGCTTAACAATTTACCATGTTAAGAGCCATTTGCAGAAATACCGACTAGCAAAATACTTACCTGACTGCTCATCCGATGAGGGGAAAAAGACTGACAAGAAAGAAACGGGGGATATGCTTTCCAATCTAGATGGTTCATCGGGGATGCAGATTACCGAAGCACTAAAGCTTCAAATGGAGGTACAGAAGCGACTACATGAACAATTGGAGGTGCAGAGACAGCTACAATTAAGGATAGAAGCTCAGGGAAAATATTTGAAAAAGATAATCGAAGAGCAACAGCGACTCAGTGGGGTTCTTTCAGAAGCACCCGGCTCTGGTGTATCAGCACCAACTCCAGGAGATATGTCCCAACAGGAGCTCGACAATAAGACTGAACCGGCAACCCCTGACCCAGAAAAAGCTGCTAAAGAGCATGCACCAGCAAAGAGTCTTTCAGCAGAATCTTTTTCATCTCACCATGAACCGATGACACCAGATTCTGGTTGCCAAGTCGGTTCTCCTGCTGACAGTCCTAATGGCGAGAGATCAACTAAAAAGCAACGAGTAAGCGTGGAAGAAGCGTATTTGAAATCAGACATGGTGCTTCCTCATCAGATACTGGAGTCAAGCATGCCATCATACCAGCAACCAACCGCTGTTTTCCTTACCCGAGATCACTTTGATCCTTCTTTAGGCATGTCTACAAGAAGTGGTGAGGAATTGGATAAGCTTGGTGGCAGGAATATGTGA
Protein sequence

>Msa0277950

MYQPTNVPDSSLIGSNPLVHGQHIESGRSAMDPGSGGNSLANNSNLNSKQRLRWTHELHERFVDAVAQLGGPDRATPKGVLRVMGVQGLTIYHVKSHLQKYRLAKYLPDCSSDEGKKTDKKETGDMLSNLDGSSGMQITEALKLQMEVQKRLHEQLEVQRQLQLRIEAQGKYLKKIIEEQQRLSGVLSEAPGSGVSAPTPGDMSQQELDNKTEPATPDPEKAAKEHAPAKSLSAESFSSHHEPMTPDSGCQVGSPADSPNGERSTKKQRVSVEEAYLKSDMVLPHQILESSMPSYQQPTAVFLTRDHFDPSLGMSTRSGEELDKLGGRNM*