AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1144240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1144240 MtrunA17_Chr2g0291021 97.273 330 9 0 1 330 1 330 0.0 663
Msa1144240 MtrunA17_Chr7g0254981 73.050 141 37 1 49 188 50 190 8.39e-69 216
Msa1144240 MtrunA17_Chr7g0261911 60.390 154 52 3 33 186 252 396 6.97e-53 181
Msa1144240 MtrunA17_Chr1g0175091 48.087 183 77 3 35 217 244 408 1.95e-51 176
Msa1144240 MtrunA17_Chr1g0190301 53.216 171 73 3 33 200 156 322 1.24e-48 167
Msa1144240 MtrunA17_Chr7g0258551 51.351 148 69 2 46 193 209 353 2.99e-47 164
Msa1144240 MtrunA17_Chr7g0274471 53.333 150 68 1 48 197 217 364 1.35e-46 163
Msa1144240 MtrunA17_Chr8g0356501 59.398 133 51 2 49 179 37 168 4.32e-44 154
Msa1144240 MtrunA17_Chr5g0409731 52.448 143 58 1 49 181 25 167 6.24e-44 152
Msa1144240 MtrunA17_Chr4g0042451 44.792 192 80 6 47 220 15 198 2.14e-43 152
Msa1144240 MtrunA17_Chr2g0310011 58.779 131 47 2 49 175 35 162 4.51e-42 149
Msa1144240 MtrunA17_Chr7g0242521 46.497 157 75 4 49 200 13 165 5.75e-41 143
Msa1144240 MtrunA17_Chr5g0417521 44.262 183 87 5 47 217 13 192 7.39e-41 146
Msa1144240 MtrunA17_Chr2g0321461 49.682 157 61 3 47 185 43 199 9.79e-41 146
Msa1144240 MtrunA17_Chr6g0461641 48.701 154 63 2 47 185 40 192 1.19e-40 146
Msa1144240 MtrunA17_Chr2g0290991 47.826 138 56 2 49 173 39 173 2.40e-34 127
Msa1144240 MtrunA17_Chr1g0197171 47.059 136 63 2 49 176 44 178 7.00e-33 123
Msa1144240 MtrunA17_Chr1g0175071 46.043 139 66 2 49 184 177 309 1.08e-30 118
Msa1144240 MtrunA17_Chr1g0175081 48.000 75 39 0 35 109 8 82 2.97e-22 89.7
Msa1144240 MtrunA17_Chr7g0258531 37.222 180 94 5 13 180 171 343 7.52e-22 94.7
Msa1144240 MtrunA17_Chr2g0288601 66.071 56 19 0 51 106 73 128 1.03e-16 75.5
Msa1144240 MtrunA17_Chr7g0243081 61.404 57 22 0 51 107 73 129 1.49e-16 79.3
Msa1144240 MtrunA17_Chr6g0479431 60.000 65 24 1 51 113 204 268 2.58e-16 79.0
Msa1144240 MtrunA17_Chr8g0366281 46.535 101 44 3 51 151 175 265 2.77e-16 78.6
Msa1144240 MtrunA17_Chr3g0095611 48.718 78 24 1 51 112 21 98 7.91e-16 75.1
Msa1144240 MtrunA17_Chr1g0198601 56.061 66 29 0 49 114 211 276 1.48e-15 76.6
Msa1144240 MtrunA17_Chr5g0410741 63.636 55 20 0 51 105 221 275 2.24e-15 76.3
Msa1144240 MtrunA17_Chr8g0379121 63.636 55 20 0 51 105 113 167 2.34e-15 76.6
Msa1144240 MtrunA17_Chr1g0162931 53.425 73 33 1 51 123 20 91 2.89e-15 75.1
Msa1144240 MtrunA17_Chr3g0109981 63.636 55 20 0 51 105 276 330 3.19e-15 76.3
Msa1144240 MtrunA17_Chr2g0320231 65.455 55 19 0 51 105 99 153 4.52e-15 75.5
Msa1144240 MtrunA17_Chr1g0154261 61.818 55 21 0 51 105 128 182 5.88e-15 74.3
Msa1144240 MtrunA17_Chr1g0210911 65.385 52 18 0 51 102 24 75 6.45e-15 74.7
Msa1144240 MtrunA17_Chr5g0402631 55.714 70 30 1 49 117 214 283 4.10e-14 72.4
Msa1144240 MtrunA17_Chr2g0283951 67.347 49 16 0 51 99 91 139 1.46e-13 70.9
Msa1144240 MtrunA17_Chr5g0422621 58.182 55 23 0 49 103 198 252 5.22e-13 68.9
Msa1144240 MtrunA17_Chr4g0062921 48.718 78 39 1 39 115 224 301 6.08e-13 69.3
Msa1144240 MtrunA17_Chr4g0009981 50.685 73 33 2 41 113 108 177 1.01e-12 67.4
Msa1144240 MtrunA17_Chr4g0045361 60.000 55 22 0 49 103 274 328 1.17e-12 68.2
Msa1144240 MtrunA17_Chr4g0009901 56.364 55 23 1 49 103 168 221 1.98e-12 66.6
Msa1144240 MtrunA17_Chr4g0031961 48.611 72 35 2 49 119 131 201 7.83e-12 65.1
Msa1144240 MtrunA17_Chr2g0324891 50.820 61 29 1 42 102 154 213 9.01e-12 65.5
Msa1144240 MtrunA17_Chr5g0400241 43.038 79 44 1 27 105 289 366 1.27e-11 65.5
Msa1144240 MtrunA17_Chr7g0255711 58.182 55 22 1 49 103 91 144 1.33e-11 64.3
Msa1144240 MtrunA17_Chr4g0009851 52.308 65 28 2 49 113 169 230 2.08e-11 63.9
Msa1144240 MtrunA17_Chr2g0304841 54.545 55 24 1 49 103 213 266 2.56e-11 64.7
Msa1144240 MtrunA17_Chr4g0067981 58.182 55 22 1 49 103 215 268 3.46e-11 64.3
Msa1144240 MtrunA17_Chr4g0040481 53.704 54 24 1 49 102 158 210 4.84e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1144240 AT2G01060.1 66.779 298 69 9 43 330 9 286 3.04e-119 345
Msa1144240 AT2G01060.2 62.646 257 66 9 84 330 1 237 1.80e-88 265
Msa1144240 AT5G29000.1 57.692 156 63 2 29 183 170 323 2.09e-51 174
Msa1144240 AT5G29000.3 57.692 156 63 2 29 183 170 323 2.09e-51 174
Msa1144240 AT5G29000.2 57.692 156 63 2 29 183 213 366 4.06e-51 175
Msa1144240 AT3G04450.4 52.273 176 76 3 44 212 193 367 7.56e-51 174
Msa1144240 AT3G04450.2 52.273 176 76 3 44 212 193 367 7.56e-51 174
Msa1144240 AT3G04450.1 52.273 176 76 3 44 212 233 407 1.34e-50 174
Msa1144240 AT4G28610.1 52.432 185 80 4 41 225 217 393 4.26e-50 172
Msa1144240 AT5G06800.1 57.534 146 57 3 37 182 182 322 6.49e-50 171
Msa1144240 AT4G13640.1 59.574 141 47 2 49 179 37 177 1.91e-49 167
Msa1144240 AT3G13040.3 50.265 189 86 4 44 227 236 421 1.98e-49 171
Msa1144240 AT3G13040.1 50.265 189 86 4 44 227 236 421 1.98e-49 171
Msa1144240 AT3G13040.2 50.265 189 86 4 44 227 236 421 1.98e-49 171
Msa1144240 AT3G24120.1 58.156 141 49 2 49 179 41 181 7.71e-49 166
Msa1144240 AT5G06800.2 55.705 149 60 3 37 182 182 327 7.97e-49 168
Msa1144240 AT1G79430.2 61.268 142 47 3 49 185 34 172 2.76e-48 166
Msa1144240 AT4G13640.2 58.333 144 47 3 49 179 37 180 3.08e-47 161
Msa1144240 AT3G24120.2 56.944 144 49 3 49 179 41 184 9.96e-47 160
Msa1144240 AT3G04030.3 48.333 180 72 4 23 185 19 194 8.16e-43 152
Msa1144240 AT1G69580.2 49.693 163 65 3 47 192 32 194 9.30e-43 151
Msa1144240 AT2G20400.2 53.165 158 71 2 49 206 231 385 1.04e-42 152
Msa1144240 AT1G69580.1 49.091 165 62 4 47 192 32 193 1.05e-42 150
Msa1144240 AT2G20400.1 53.797 158 69 2 49 206 231 384 1.33e-42 152
Msa1144240 AT5G18240.1 51.316 152 61 1 47 185 43 194 4.04e-42 150
Msa1144240 AT5G18240.4 51.316 152 61 1 47 185 43 194 4.04e-42 150
Msa1144240 AT3G04030.2 47.778 180 72 5 23 185 19 193 3.73e-40 145
Msa1144240 AT5G18240.5 50.000 152 61 2 47 185 43 192 7.33e-39 142
Msa1144240 AT5G45580.1 45.223 157 56 3 49 182 7 156 4.34e-37 134
Msa1144240 AT3G04030.1 45.556 180 71 5 23 185 19 188 6.82e-37 136
Msa1144240 AT5G45580.2 45.860 157 55 3 49 182 23 172 8.00e-37 134
Msa1144240 AT5G18240.2 48.026 152 60 2 47 185 43 188 3.20e-36 135
Msa1144240 AT5G18240.3 48.026 152 60 2 47 185 43 188 3.20e-36 135
Msa1144240 AT3G12730.1 47.826 138 64 4 49 179 23 159 3.09e-35 128
Msa1144240 AT1G79430.1 54.545 110 42 3 81 185 1 107 4.73e-27 108
Msa1144240 AT5G29000.4 60.674 89 33 1 29 117 213 299 3.09e-25 103
Msa1144240 AT3G04450.3 64.286 70 25 0 44 113 233 302 3.48e-25 103
Msa1144240 AT3G04030.5 49.153 118 47 2 81 185 1 118 1.34e-24 102
Msa1144240 AT5G06800.3 60.563 71 26 1 45 115 188 256 5.54e-24 99.8
Msa1144240 AT3G04030.4 44.915 118 46 3 81 185 1 112 4.54e-19 86.7
Msa1144240 AT5G18240.6 43.220 118 48 2 81 185 1 112 5.52e-19 86.7
Msa1144240 AT2G06020.1 59.649 57 23 0 49 105 86 142 7.10e-18 83.2
Msa1144240 AT2G42660.1 65.517 58 20 0 51 108 52 109 2.94e-17 80.5
Msa1144240 AT2G40260.1 57.333 75 20 1 51 125 84 146 3.08e-17 82.4
Msa1144240 AT4G04605.1 69.231 52 16 0 51 102 16 67 4.16e-17 79.3
Msa1144240 AT1G32240.1 43.396 106 51 2 51 156 215 311 1.79e-16 80.1
Msa1144240 AT1G32240.2 43.396 106 51 2 51 156 215 311 1.99e-16 79.7
Msa1144240 AT5G42630.2 57.576 66 28 0 51 116 107 172 4.46e-16 76.6
Msa1144240 AT5G42630.1 57.576 66 28 0 51 116 107 172 5.78e-16 77.4
Msa1144240 AT3G12730.2 39.623 106 56 4 81 179 1 105 1.29e-15 74.3
Msa1144240 AT5G16560.1 60.000 65 24 1 51 113 221 285 1.62e-15 77.4
Msa1144240 AT1G14600.1 69.231 52 16 0 51 102 25 76 2.03e-15 75.5
Msa1144240 AT4G17695.1 63.636 55 20 0 51 105 166 220 3.12e-15 75.9
Msa1144240 AT2G02060.2 69.231 52 16 0 51 102 31 82 3.28e-15 74.7
Msa1144240 AT2G02060.1 69.231 52 16 0 51 102 31 82 3.28e-15 74.7
Msa1144240 AT2G38300.1 63.636 55 20 0 51 105 56 110 3.76e-15 75.9
Msa1144240 AT4G37180.1 60.000 55 22 0 49 103 210 264 3.44e-14 73.2
Msa1144240 AT4G37180.2 60.000 55 22 0 49 103 217 271 4.20e-14 72.8
Msa1144240 AT4G04580.1 54.545 55 25 0 51 105 16 70 1.20e-13 68.6
Msa1144240 AT1G49560.1 54.545 66 30 0 49 114 192 257 1.52e-13 70.9
Msa1144240 AT3G25790.1 58.182 55 23 0 54 108 201 255 2.98e-13 70.1
Msa1144240 AT1G25550.1 55.385 65 29 0 44 108 204 268 5.27e-13 69.3
Msa1144240 AT3G10760.1 48.101 79 40 1 25 103 81 158 7.96e-13 68.9
Msa1144240 AT1G13300.1 44.086 93 41 2 16 108 159 240 1.40e-12 68.2
Msa1144240 AT1G68670.1 48.052 77 40 0 42 118 208 284 1.82e-12 67.8
Msa1144240 AT5G05090.1 60.377 53 20 1 51 103 83 134 4.25e-12 65.9
Msa1144240 AT4G37180.3 55.172 58 26 0 49 106 210 267 6.17e-12 65.5
Msa1144240 AT5G58080.1 52.174 69 28 3 49 117 194 257 7.07e-12 66.6
Msa1144240 AT5G59570.2 58.491 53 21 1 51 103 143 194 9.86e-12 65.1
Msa1144240 AT5G59570.1 58.491 53 21 1 51 103 143 194 9.86e-12 65.1
Msa1144240 AT3G46640.2 58.491 53 21 1 51 103 146 197 1.21e-11 65.1
Msa1144240 AT3G46640.1 58.491 53 21 1 51 103 146 197 1.21e-11 65.1
Msa1144240 AT3G46640.3 58.491 53 21 1 51 103 146 197 1.24e-11 65.1
Msa1144240 AT2G20570.1 51.471 68 31 2 35 102 140 205 1.35e-11 65.5
Msa1144240 AT2G03500.1 53.226 62 29 0 47 108 231 292 1.63e-11 65.5
Msa1144240 AT2G01760.3 45.333 75 38 2 49 123 135 206 1.66e-11 64.7
Msa1144240 AT5G44190.1 46.154 65 34 1 38 102 136 199 2.79e-11 64.3
Msa1144240 AT2G01760.1 45.333 75 38 2 49 123 200 271 2.96e-11 64.3
Msa1144240 AT5G44190.2 46.154 65 34 1 38 102 136 199 3.20e-11 63.9
Msa1144240 AT2G01760.2 45.333 75 38 2 49 123 228 299 4.22e-11 63.9
Msa1144240 AT2G40970.1 56.364 55 23 1 49 103 105 158 4.60e-11 62.8
Msa1144240 AT3G25790.2 58.696 46 19 0 54 99 201 246 9.79e-11 62.0

Find 99 sgRNAs with CRISPR-Local

Find 301 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCCTGATTCGAGTTTAAT+TGG 0.223090 7_4:-20182481 Msa1144240:CDS
CTCTAAAAGTAATTAAATTA+AGG 0.260834 7_4:+20180383 None:intergenic
GATCACTTTGATCCTTCTTT+AGG 0.297515 7_4:-20180592 Msa1144240:CDS
TTTAGAGAACTGATTTGAAT+TGG 0.300478 7_4:-20180367 Msa1144240:three_prime_UTR
CCATGTTAAGAGCCATTTGC+AGG 0.349218 7_4:-20182039 Msa1144240:intron
TACAAGAAGTGGTGAGGAAT+TGG 0.349818 7_4:-20180563 Msa1144240:CDS
TGCAGAGACAGCTACAATTA+AGG 0.349960 7_4:-20181035 Msa1144240:CDS
TGGATTTCATAGATCTCATC+TGG 0.363640 7_4:-20184369 Msa1144240:five_prime_UTR
CTTCCAATTAAACTCGAATC+AGG 0.368180 7_4:+20182478 None:intergenic
TTAGTTGATCTCTCGCCATT+AGG 0.374486 7_4:+20180733 None:intergenic
CATAACTCTGAGGACACCTT+TGG 0.375653 7_4:+20182085 None:intergenic
CAAAGGTGTCCTCAGAGTTA+TGG 0.377183 7_4:-20182084 Msa1144240:CDS
ATGTGCTGACCATGAACTAA+TGG 0.385679 7_4:+20182451 None:intergenic
GAACCGATGACACCAGATTC+TGG 0.386395 7_4:-20180784 Msa1144240:CDS
AAGACTGACAAGAAAGAAAC+GGG 0.390608 7_4:-20181741 Msa1144240:CDS
TGAGTTGAGATTAGAGTTGT+TGG 0.390955 7_4:+20182378 None:intergenic
GGAAAACAGCGGTTGGTTGC+TGG 0.394253 7_4:+20180623 None:intergenic
AAAGGTGTCCTCAGAGTTAT+GGG 0.402741 7_4:-20182083 Msa1144240:CDS
ACAATTAAGGATAGAAGCTC+AGG 0.410929 7_4:-20181022 Msa1144240:CDS
GGAAGCAATCCATTAGTTCA+TGG 0.415844 7_4:-20182460 Msa1144240:CDS
TTAGAGAACTGATTTGAATT+GGG 0.421756 7_4:-20180366 Msa1144240:three_prime_UTR
CATTTGAAGCTTTAGTGCTT+CGG 0.424328 7_4:+20181361 None:intergenic
GATATGCTTTCCAATCTAGA+TGG 0.424452 7_4:-20181717 Msa1144240:CDS
TCCTGTTGGGACATATCTCC+TGG 0.428112 7_4:+20180910 None:intergenic
TTATATGCATTCTTAGTTGA+TGG 0.434188 7_4:-20180474 Msa1144240:three_prime_UTR
TCTGCTGAAAGACTCTTTGC+TGG 0.439579 7_4:+20180826 None:intergenic
GGTGCTTCCTCATCAGATAC+TGG 0.444138 7_4:-20180665 Msa1144240:CDS
CTTTCAGAAGCACCCGGCTC+TGG 0.447624 7_4:-20180949 Msa1144240:CDS
CCGACTTGGCAACCAGAATC+TGG 0.448662 7_4:+20180772 None:intergenic
CTCGAATCAGGAACATTAGT+TGG 0.450860 7_4:+20182490 None:intergenic
TCTCCTGCTGACAGTCCTAA+TGG 0.454643 7_4:-20180748 Msa1144240:CDS
TCACGATGAACCATCTAGAT+TGG 0.455940 7_4:+20181707 None:intergenic
GTTGATGCTGTGGCTCAACT+TGG 0.466308 7_4:-20182313 Msa1144240:CDS
AAGAAGGATCAAAGTGATCT+CGG 0.468691 7_4:+20180596 None:intergenic
GAAGCGACTACATGAACAAT+TGG 0.469698 7_4:-20181330 Msa1144240:CDS
CGAAGCACTAAAGCTTCAAA+TGG 0.471790 7_4:-20181360 Msa1144240:CDS
TCCAGGAGATATGTCCCAAC+AGG 0.476349 7_4:-20180911 Msa1144240:CDS
ATAACTCTGAGGACACCTTT+GGG 0.476739 7_4:+20182086 None:intergenic
ATTAAATTGGTAGCCCAAGA+TGG 0.479332 7_4:+20180288 None:intergenic
TCTTATTGTCGAGCTCCTGT+TGG 0.481895 7_4:+20180896 None:intergenic
CTTATTGTCGAGCTCCTGTT+GGG 0.485642 7_4:+20180897 None:intergenic
CTCAGAGTTATGGGTGTACA+AGG 0.485843 7_4:-20182074 Msa1144240:CDS
GGTAGGAGCGCTATGGATCC+TGG 0.493466 7_4:-20182421 Msa1144240:CDS
CTACACAGCTCCGAAATTGA+AGG 0.498072 7_4:-20184416 Msa1144240:five_prime_UTR
CATGGTCAGCACATAGAGTC+TGG 0.500025 7_4:-20182442 Msa1144240:CDS
GAGTGCACATTACCTGCAAA+TGG 0.500488 7_4:+20182027 None:intergenic
AAAGACTGACAAGAAAGAAA+CGG 0.503951 7_4:-20181742 Msa1144240:CDS
TCCTTAGGTGCCACACCCAA+AGG 0.508503 7_4:-20182101 Msa1144240:intron
GATGCTGTGGCTCAACTTGG+TGG 0.512420 7_4:-20182310 Msa1144240:CDS
GCTCGACAATAAGACTGAAC+CGG 0.517717 7_4:-20180887 Msa1144240:CDS
TGGTGCTGATACACCAGAGC+CGG 0.522258 7_4:+20180936 None:intergenic
AATCTGGTGTCATCGGTTCA+TGG 0.523061 7_4:+20180788 None:intergenic
GATCAAAGTGATCTCGGGTA+AGG 0.525714 7_4:+20180602 None:intergenic
CCTGCAAATGGCTCTTAACA+TGG 0.525821 7_4:+20182039 None:intergenic
CCAGATTCTGGTTGCCAAGT+CGG 0.528854 7_4:-20180772 Msa1144240:CDS
GTACCATCGGATGAGCAGTC+AGG 0.531157 7_4:+20181907 None:intergenic
ACTCAAAGCAACGTTTGCGT+TGG 0.531602 7_4:-20182360 Msa1144240:CDS
AGAAGGATCAAAGTGATCTC+GGG 0.531953 7_4:+20180597 None:intergenic
GGTGTATCAGCACCAACTCC+AGG 0.533448 7_4:-20180928 Msa1144240:CDS
AGCGCTATGGATCCTGGTAG+TGG 0.534994 7_4:-20182415 Msa1144240:CDS
CTTGTAGACATGCCTAAAGA+AGG 0.541592 7_4:+20180580 None:intergenic
TTAGGCATGTCTACAAGAAG+TGG 0.546196 7_4:-20180574 Msa1144240:CDS
CGGGTAAGGAAAACAGCGGT+TGG 0.546421 7_4:+20180616 None:intergenic
GGTGCTGATACACCAGAGCC+GGG 0.552630 7_4:+20180937 None:intergenic
TCTGAGGACACCTTTGGGTG+TGG 0.554653 7_4:+20182091 None:intergenic
CAACCAGAATCTGGTGTCAT+CGG 0.558599 7_4:+20180781 None:intergenic
AGAGTCTGGTAGGAGCGCTA+TGG 0.562642 7_4:-20182428 Msa1144240:CDS
GCGGTTGGTTGCTGGTATGA+TGG 0.564870 7_4:+20180631 None:intergenic
TGTCAGCAGGAGAACCGACT+TGG 0.567410 7_4:+20180758 None:intergenic
AATTGGATAAGCTTGGTGGC+AGG 0.568213 7_4:-20180546 Msa1144240:CDS
TGGGACATATCTCCTGGAGT+TGG 0.568536 7_4:+20180916 None:intergenic
TTAAATTGGTAGCCCAAGAT+GGG 0.568543 7_4:+20180289 None:intergenic
AGACTGTTTCCTCCACTACC+AGG 0.568775 7_4:+20182403 None:intergenic
AAGAGCAACAGCGACTCAGT+GGG 0.575024 7_4:-20180975 Msa1144240:CDS
TCGCCATTAGGACTGTCAGC+AGG 0.575904 7_4:+20180745 None:intergenic
TACACAGCTCCGAAATTGAA+GGG 0.581590 7_4:-20184415 Msa1144240:five_prime_UTR
TATGCATTCTTAGTTGATGG+AGG 0.586098 7_4:-20180471 Msa1144240:three_prime_UTR
GGTGAGGAATTGGATAAGCT+TGG 0.592014 7_4:-20180553 Msa1144240:CDS
TTACCTGACTGCTCATCCGA+TGG 0.595863 7_4:-20181910 Msa1144240:intron
GCGACTACATGAACAATTGG+AGG 0.598747 7_4:-20181327 Msa1144240:intron
TGAACGCTTTGTTGATGCTG+TGG 0.601507 7_4:-20182323 Msa1144240:CDS
GGGGTTCTTTCAGAAGCACC+CGG 0.619430 7_4:-20180955 Msa1144240:CDS
GGATTTCATAGATCTCATCT+GGG 0.628659 7_4:-20184368 Msa1144240:intron
GTCAGCACATAGAGTCTGGT+AGG 0.630464 7_4:-20182438 Msa1144240:CDS
AGCACTAAAGCTTCAAATGG+AGG 0.636135 7_4:-20181357 Msa1144240:CDS
GAAGAGCAACAGCGACTCAG+TGG 0.642615 7_4:-20180976 Msa1144240:CDS
CTTGACTCCAGTATCTGATG+AGG 0.648174 7_4:+20180658 None:intergenic
CATGTCTACAAGAAGTGGTG+AGG 0.650625 7_4:-20180569 Msa1144240:CDS
GAGGAATTGGATAAGCTTGG+TGG 0.651592 7_4:-20180550 Msa1144240:CDS
CAATTAAGGATAGAAGCTCA+GGG 0.654933 7_4:-20181021 Msa1144240:CDS
GGGGAAAATACTCACGATCG+GGG 0.658151 7_4:+20182287 None:intergenic
GCTATGGATCCTGGTAGTGG+AGG 0.662575 7_4:-20182412 Msa1144240:CDS
ACACAGCTCCGAAATTGAAG+GGG 0.667923 7_4:-20184414 Msa1144240:five_prime_UTR
GACTGACAAGAAAGAAACGG+GGG 0.678708 7_4:-20181739 Msa1144240:CDS
AGCGTATTTGAAATCAGACA+TGG 0.689566 7_4:-20180686 Msa1144240:CDS
AGAGCAACAGCGACTCAGTG+GGG 0.711050 7_4:-20180974 Msa1144240:CDS
CTTGTACACCCATAACTCTG+AGG 0.715783 7_4:+20182075 None:intergenic
ATCTCGGGTAAGGAAAACAG+CGG 0.741797 7_4:+20180612 None:intergenic
AGACTGACAAGAAAGAAACG+GGG 0.753105 7_4:-20181740 Msa1144240:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAAATAAATAAATGTTAAT+TGG - chr7_4:20180842-20180861 Msa1144240:CDS 10.0%
!!! TTTTATTAATTTCAATCAAA+TGG - chr7_4:20181931-20181950 Msa1144240:CDS 10.0%
!!! TTGTATTGTTTTTACATTTT+TGG - chr7_4:20180550-20180569 Msa1144240:CDS 15.0%
!!! AAGTTTGATTTTTTAAGTTT+TGG - chr7_4:20180638-20180657 Msa1144240:CDS 15.0%
!! ATACAATATTAACAACAATT+AGG + chr7_4:20180817-20180836 None:intergenic 15.0%
!! ATAAATAAATGTTAATTGGT+TGG - chr7_4:20180846-20180865 Msa1144240:CDS 15.0%
!! ATTAAATTTATGATCTATTG+TGG - chr7_4:20181742-20181761 Msa1144240:CDS 15.0%
!!! GATTTAAAAACTTTTAGATA+AGG + chr7_4:20182649-20182668 None:intergenic 15.0%
!! CATTAAAAAAACAAGAATAA+TGG + chr7_4:20182803-20182822 None:intergenic 15.0%
!!! TAAAAAGTTATGTTTCTTTT+AGG - chr7_4:20183262-20183281 Msa1144240:intron 15.0%
!!! TTTTATTTTGAATCAAGTAA+AGG - chr7_4:20183651-20183670 Msa1144240:intron 15.0%
!!! CTCTAAAAGTAATTAAATTA+AGG + chr7_4:20184403-20184422 None:intergenic 15.0%
!!! AAAGCATTCATTGATTTTTA+GGG - chr7_4:20180945-20180964 Msa1144240:CDS 20.0%
!! ATCACTAAGATAACATATAT+AGG + chr7_4:20181073-20181092 None:intergenic 20.0%
!!! AGTAATTTGATGATATTTGA+AGG - chr7_4:20181237-20181256 Msa1144240:intron 20.0%
!!! GTAATTTGATGATATTTGAA+GGG - chr7_4:20181238-20181257 Msa1144240:intron 20.0%
!!! TAATTTGATGATATTTGAAG+GGG - chr7_4:20181239-20181258 Msa1144240:intron 20.0%
!!! CTTTTTTTAATGACAAATGT+TGG - chr7_4:20181448-20181467 Msa1144240:intron 20.0%
!!! ACAGTTTGAAAAGTTTTTTA+TGG - chr7_4:20182037-20182056 Msa1144240:intron 20.0%
!! AAATCAGTAAGATCTAAATA+TGG + chr7_4:20182925-20182944 None:intergenic 20.0%
!! AGAATGCTTAATACTTTAAA+CGG + chr7_4:20182960-20182979 None:intergenic 20.0%
!! AAAGTTATCAGCAAATTTAA+AGG + chr7_4:20182999-20183018 None:intergenic 20.0%
!!! ACTTTTTAATTGTCTTATAG+AGG - chr7_4:20183014-20183033 Msa1144240:intron 20.0%
!!! CTTTTTAATTGTCTTATAGA+GGG - chr7_4:20183015-20183034 Msa1144240:intron 20.0%
!!! TTTTTAATTGTCTTATAGAG+GGG - chr7_4:20183016-20183035 Msa1144240:intron 20.0%
!! TATCATAATTATACAAGGAA+TGG - chr7_4:20183126-20183145 Msa1144240:intron 20.0%
!! TAGAAAAAATAACAGTTACA+AGG + chr7_4:20183717-20183736 None:intergenic 20.0%
!!! TTATTTTTTCTATGTGAAAC+AGG - chr7_4:20183725-20183744 Msa1144240:intron 20.0%
!! GAAAATTTTGTATACGTAGT+TGG - chr7_4:20180394-20180413 Msa1144240:three_prime_UTR 25.0%
! TTATAGAAATGAAGAAGAGA+AGG + chr7_4:20180485-20180504 None:intergenic 25.0%
! GAGAAAGTAAGGAATAAAAA+GGG + chr7_4:20180508-20180527 None:intergenic 25.0%
! AGAGAAAGTAAGGAATAAAA+AGG + chr7_4:20180509-20180528 None:intergenic 25.0%
! TAAAACCAAATAAGCCAAAA+GGG + chr7_4:20180579-20180598 None:intergenic 25.0%
! GCTTTCAAAAAGTTAAAACA+AGG + chr7_4:20180930-20180949 None:intergenic 25.0%
!!! GAAAGCATTCATTGATTTTT+AGG - chr7_4:20180944-20180963 Msa1144240:CDS 25.0%
! GATGTTAATGTGATGAAAAA+TGG - chr7_4:20180986-20181005 Msa1144240:CDS 25.0%
! TGTTATCTTAGTGATTAATC+AGG - chr7_4:20181078-20181097 Msa1144240:intron 25.0%
! TAGCAAAGAATCTATCTAAT+AGG + chr7_4:20181266-20181285 None:intergenic 25.0%
! AAATTGAATTCAACTTTCTG+TGG - chr7_4:20181345-20181364 Msa1144240:CDS 25.0%
!! TAGTTTAAGTTTCTTAGACA+TGG - chr7_4:20181623-20181642 Msa1144240:intron 25.0%
!! GTGTTTGTTAAATACTGTAT+TGG - chr7_4:20181972-20181991 Msa1144240:intron 25.0%
! CGTTGAAAAAAATTGTACAA+CGG + chr7_4:20182017-20182036 None:intergenic 25.0%
! AACTGAATCTGATTAAGAAT+TGG - chr7_4:20182100-20182119 Msa1144240:intron 25.0%
! ATTTCAGCTAACGAAAAAAT+GGG + chr7_4:20182519-20182538 None:intergenic 25.0%
! TTGGTTACATCATACATATT+TGG - chr7_4:20182552-20182571 Msa1144240:intron 25.0%
!! CAGATATCAAATACTTTTGT+TGG - chr7_4:20182577-20182596 Msa1144240:intron 25.0%
! TAAATTCATCATCTAGCATT+AGG + chr7_4:20182611-20182630 None:intergenic 25.0%
! TGGGTTATCATAATTATACA+AGG - chr7_4:20183121-20183140 Msa1144240:intron 25.0%
!!! TATAGATTTTTTTCCACCAA+CGG + chr7_4:20183305-20183324 None:intergenic 25.0%
! TTATATGCATTCTTAGTTGA+TGG - chr7_4:20184309-20184328 Msa1144240:intron 25.0%
!!! TTTAGAGAACTGATTTGAAT+TGG - chr7_4:20184416-20184435 Msa1144240:five_prime_UTR 25.0%
!!! TTAGAGAACTGATTTGAATT+GGG - chr7_4:20184417-20184436 Msa1144240:five_prime_UTR 25.0%
! AAAAACTCAACCTTGAAAAT+GGG + chr7_4:20184471-20184490 None:intergenic 25.0%
!!! ATTTTCAAGGTTGAGTTTTT+GGG - chr7_4:20184471-20184490 Msa1144240:five_prime_UTR 25.0%
TGAAAGAAAGAGAGAAAGTA+AGG + chr7_4:20180519-20180538 None:intergenic 30.0%
CTAAAACCAAATAAGCCAAA+AGG + chr7_4:20180580-20180599 None:intergenic 30.0%
! TTTTCAGCTTTTGTTGATGT+GGG - chr7_4:20180752-20180771 Msa1144240:CDS 30.0%
ATAGCTAACATCAGTGAATT+GGG + chr7_4:20181029-20181048 None:intergenic 30.0%
TATAGCTAACATCAGTGAAT+TGG + chr7_4:20181030-20181049 None:intergenic 30.0%
TTCACTGATGTTAGCTATAA+AGG - chr7_4:20181031-20181050 Msa1144240:CDS 30.0%
!!! ATTTTCAGCTCCACTTTTTA+GGG - chr7_4:20181125-20181144 Msa1144240:intron 30.0%
TATCTAGAGATAGATGATGA+TGG + chr7_4:20181323-20181342 None:intergenic 30.0%
!!! TTGATTTTTTCTCCTTTGTC+CGG - chr7_4:20181486-20181505 Msa1144240:intron 30.0%
ACTTGGTAAACTTCTTGTAT+GGG + chr7_4:20181571-20181590 None:intergenic 30.0%
AACTTGGTAAACTTCTTGTA+TGG + chr7_4:20181572-20181591 None:intergenic 30.0%
TTCTAAGTCAAACTAGAACT+TGG + chr7_4:20181588-20181607 None:intergenic 30.0%
!! AGTTCTAGTTTGACTTAGAA+TGG - chr7_4:20181588-20181607 Msa1144240:intron 30.0%
AAACTATTATTAGTGAACCC+GGG + chr7_4:20181856-20181875 None:intergenic 30.0%
CAAACTATTATTAGTGAACC+CGG + chr7_4:20181857-20181876 None:intergenic 30.0%
ATTTCAATCAAATGGAGCAT+TGG - chr7_4:20181939-20181958 Msa1144240:intron 30.0%
AAATTGTACAACGGATCATT+TGG + chr7_4:20182008-20182027 None:intergenic 30.0%
AACTCTCTTTCTTTCAATCA+TGG + chr7_4:20182079-20182098 None:intergenic 30.0%
ATTGGAATTAGAACATGTTG+AGG - chr7_4:20182118-20182137 Msa1144240:intron 30.0%
TTGGAATTAGAACATGTTGA+GGG - chr7_4:20182119-20182138 Msa1144240:intron 30.0%
AGACAGAAACACACAAAAAT+TGG + chr7_4:20182146-20182165 None:intergenic 30.0%
!! AAAGGTTGGTAAAGAAATGA+GGG + chr7_4:20182188-20182207 None:intergenic 30.0%
!! AAAAGGTTGGTAAAGAAATG+AGG + chr7_4:20182189-20182208 None:intergenic 30.0%
ACTTAATTGCAGATCGTAAA+AGG + chr7_4:20182206-20182225 None:intergenic 30.0%
TTTCAGCTAACGAAAAAATG+GGG + chr7_4:20182518-20182537 None:intergenic 30.0%
CATTTCAGCTAACGAAAAAA+TGG + chr7_4:20182520-20182539 None:intergenic 30.0%
TGAAATGTAATGCGTAGAAT+TGG - chr7_4:20182533-20182552 Msa1144240:intron 30.0%
TGCTAGATGATGAATTTATC+AGG - chr7_4:20182613-20182632 Msa1144240:intron 30.0%
! TCTATACTATCCTTTTCCTT+AGG - chr7_4:20182667-20182686 Msa1144240:intron 30.0%
!! ATAATGGATACCACATTAAG+AGG + chr7_4:20182787-20182806 None:intergenic 30.0%
ATTTAAAGGAGGAAACAAAG+TGG + chr7_4:20182985-20183004 None:intergenic 30.0%
GTTATCAGCAAATTTAAAGG+AGG + chr7_4:20182996-20183015 None:intergenic 30.0%
AAAGACTGACAAGAAAGAAA+CGG - chr7_4:20183041-20183060 Msa1144240:intron 30.0%
TGATAACCCAAAAGCAAAAA+GGG + chr7_4:20183111-20183130 None:intergenic 30.0%
ATGATAACCCAAAAGCAAAA+AGG + chr7_4:20183112-20183131 None:intergenic 30.0%
CTAAATAGAACCATCTCATT+TGG - chr7_4:20183198-20183217 Msa1144240:intron 30.0%
!!! ATAGATTTTTTTCCACCAAC+GGG + chr7_4:20183304-20183323 None:intergenic 30.0%
TAATAATGTTACATGTCTGC+AGG - chr7_4:20183386-20183405 Msa1144240:intron 30.0%
AATAATGTTACATGTCTGCA+GGG - chr7_4:20183387-20183406 Msa1144240:intron 30.0%
GTGAGATGAAATGATGATAT+TGG - chr7_4:20183566-20183585 Msa1144240:intron 30.0%
AAGGAATAATAGACAGAATG+AGG - chr7_4:20183670-20183689 Msa1144240:intron 30.0%
AGGAATAATAGACAGAATGA+GGG - chr7_4:20183671-20183690 Msa1144240:intron 30.0%
CAAAAACTCAACCTTGAAAA+TGG + chr7_4:20184472-20184491 None:intergenic 30.0%
!! CATTTTCAAGGTTGAGTTTT+TGG - chr7_4:20184470-20184489 Msa1144240:five_prime_UTR 30.0%
! ATTTTCGTCCCCTTCAATTT+CGG + chr7_4:20180380-20180399 None:intergenic 35.0%
TGGATTTCATAGATCTCATC+TGG - chr7_4:20180414-20180433 Msa1144240:three_prime_UTR 35.0%
GGATTTCATAGATCTCATCT+GGG - chr7_4:20180415-20180434 Msa1144240:three_prime_UTR 35.0%
!!! TACATTTTTGGTGACCCTTT+TGG - chr7_4:20180562-20180581 Msa1144240:CDS 35.0%
AGCACAAAACTACACAAGAA+GGG + chr7_4:20180684-20180703 None:intergenic 35.0%
! CTTTTCAGCTTTTGTTGATG+TGG - chr7_4:20180751-20180770 Msa1144240:CDS 35.0%
! TTTCAGCTTTTGTTGATGTG+GGG - chr7_4:20180753-20180772 Msa1144240:CDS 35.0%
AAAACCCATAACACTCATCA+TGG + chr7_4:20180902-20180921 None:intergenic 35.0%
ACTGATGTTAGCTATAAAGG+TGG - chr7_4:20181034-20181053 Msa1144240:CDS 35.0%
!!! GATTTTCAGCTCCACTTTTT+AGG - chr7_4:20181124-20181143 Msa1144240:intron 35.0%
AAACCCTAAACCCTAAAAAG+TGG + chr7_4:20181138-20181157 None:intergenic 35.0%
!!! ACTTTTTAGGGTTTAGGGTT+TGG - chr7_4:20181137-20181156 Msa1144240:intron 35.0%
!!! CTTTTTAGGGTTTAGGGTTT+GGG - chr7_4:20181138-20181157 Msa1144240:intron 35.0%
!!! TTTTTAGGGTTTAGGGTTTG+GGG - chr7_4:20181139-20181158 Msa1144240:intron 35.0%
!!! TGATTTTTTCTCCTTTGTCC+GGG - chr7_4:20181487-20181506 Msa1144240:intron 35.0%
!! TCAATTGGTTTGACCTAAGA+AGG + chr7_4:20181526-20181545 None:intergenic 35.0%
TTTCCTAGAGATACAAACGA+AGG - chr7_4:20181678-20181697 Msa1144240:intron 35.0%
!!! AAATGGAGCATTGGTTTTCT+GGG - chr7_4:20181948-20181967 Msa1144240:intron 35.0%
TGGAATTAGAACATGTTGAG+GGG - chr7_4:20182120-20182139 Msa1144240:intron 35.0%
!! AAGGTTGGTAAAGAAATGAG+GGG + chr7_4:20182187-20182206 None:intergenic 35.0%
AATTGCAGATCGTAAAAGGT+TGG + chr7_4:20182202-20182221 None:intergenic 35.0%
! GTTCCTGATTCGAGTTTAAT+TGG - chr7_4:20182302-20182321 Msa1144240:CDS 35.0%
CTTCCAATTAAACTCGAATC+AGG + chr7_4:20182308-20182327 None:intergenic 35.0%
! TGAGTTGAGATTAGAGTTGT+TGG + chr7_4:20182408-20182427 None:intergenic 35.0%
! CAGGTAAGTATTTTGCTAGT+CGG + chr7_4:20182860-20182879 None:intergenic 35.0%
AAATCAGATTGACGTACCAT+CGG + chr7_4:20182892-20182911 None:intergenic 35.0%
AAGACTGACAAGAAAGAAAC+GGG - chr7_4:20183042-20183061 Msa1144240:intron 35.0%
GATATGCTTTCCAATCTAGA+TGG - chr7_4:20183066-20183085 Msa1144240:intron 35.0%
! ATAGCTTCCCTTTTTGCTTT+TGG - chr7_4:20183101-20183120 Msa1144240:intron 35.0%
!! TAGCTTCCCTTTTTGCTTTT+GGG - chr7_4:20183102-20183121 Msa1144240:intron 35.0%
! GAGATGGTTCTATTTAGTTC+AGG + chr7_4:20183195-20183214 None:intergenic 35.0%
GGATGTAAAACCAAATGAGA+TGG + chr7_4:20183211-20183230 None:intergenic 35.0%
!!! TCTCATTTGGTTTTACATCC+TGG - chr7_4:20183211-20183230 Msa1144240:intron 35.0%
ATAATGTTACATGTCTGCAG+GGG - chr7_4:20183388-20183407 Msa1144240:intron 35.0%
! CATTTGAAGCTTTAGTGCTT+CGG + chr7_4:20183425-20183444 None:intergenic 35.0%
ACAATTAAGGATAGAAGCTC+AGG - chr7_4:20183761-20183780 Msa1144240:intron 35.0%
CAATTAAGGATAGAAGCTCA+GGG - chr7_4:20183762-20183781 Msa1144240:intron 35.0%
! AGCGTATTTGAAATCAGACA+TGG - chr7_4:20184097-20184116 Msa1144240:intron 35.0%
AAGAAGGATCAAAGTGATCT+CGG + chr7_4:20184190-20184209 None:intergenic 35.0%
GATCACTTTGATCCTTCTTT+AGG - chr7_4:20184191-20184210 Msa1144240:intron 35.0%
! TGTGATTTTGTGTAATCTGC+AGG - chr7_4:20184264-20184283 Msa1144240:intron 35.0%
! GTGATTTTGTGTAATCTGCA+GGG - chr7_4:20184265-20184284 Msa1144240:intron 35.0%
!! TATGCATTCTTAGTTGATGG+AGG - chr7_4:20184312-20184331 Msa1144240:intron 35.0%
! GTCATATGTTCCCATTTTCA+AGG - chr7_4:20184458-20184477 Msa1144240:five_prime_UTR 35.0%
TACACAGCTCCGAAATTGAA+GGG - chr7_4:20180368-20180387 Msa1144240:three_prime_UTR 40.0%
CAGCACAAAACTACACAAGA+AGG + chr7_4:20180685-20180704 None:intergenic 40.0%
!!! TGTGTAGTTTTGTGCTGTGT+TGG - chr7_4:20180688-20180707 Msa1144240:CDS 40.0%
! TTCAGCTTTTGTTGATGTGG+GGG - chr7_4:20180754-20180773 Msa1144240:CDS 40.0%
!!! TTGCTGGGTTTTTTCTGTTG+TGG - chr7_4:20180782-20180801 Msa1144240:CDS 40.0%
!!! TGCTGGGTTTTTTCTGTTGT+GGG - chr7_4:20180783-20180802 Msa1144240:CDS 40.0%
TTCAGCCATGATGAGTGTTA+TGG - chr7_4:20180894-20180913 Msa1144240:CDS 40.0%
TCAGCCATGATGAGTGTTAT+GGG - chr7_4:20180895-20180914 Msa1144240:CDS 40.0%
!!! AGCTCCACTTTTTAGGGTTT+AGG - chr7_4:20181131-20181150 Msa1144240:intron 40.0%
!!! GCTCCACTTTTTAGGGTTTA+GGG - chr7_4:20181132-20181151 Msa1144240:intron 40.0%
!!! TTTTAGGGTTTAGGGTTTGG+GGG - chr7_4:20181140-20181159 Msa1144240:intron 40.0%
ACGAAAGGTTACCTATGCTA+GGG - chr7_4:20181175-20181194 Msa1144240:intron 40.0%
TAACATCCTTTCCCTAGCAT+AGG + chr7_4:20181189-20181208 None:intergenic 40.0%
TTTGAACCCAAGACCTTCTT+AGG - chr7_4:20181510-20181529 Msa1144240:intron 40.0%
GTTTGACCTAAGAAGGTCTT+GGG + chr7_4:20181519-20181538 None:intergenic 40.0%
AAACTTCTTGTATGGGTGGA+TGG + chr7_4:20181564-20181583 None:intergenic 40.0%
TGGTAAACTTCTTGTATGGG+TGG + chr7_4:20181568-20181587 None:intergenic 40.0%
AAAGTCAAGCCTCATGGAAT+AGG + chr7_4:20181659-20181678 None:intergenic 40.0%
TAGGAAAAAGTCAAGCCTCA+TGG + chr7_4:20181665-20181684 None:intergenic 40.0%
CATCCTTCGTTTGTATCTCT+AGG + chr7_4:20181684-20181703 None:intergenic 40.0%
!!! CAAATGGAGCATTGGTTTTC+TGG - chr7_4:20181947-20181966 Msa1144240:intron 40.0%
!!! AATGGAGCATTGGTTTTCTG+GGG - chr7_4:20181949-20181968 Msa1144240:intron 40.0%
CTCGAATCAGGAACATTAGT+TGG + chr7_4:20182296-20182315 None:intergenic 40.0%
GGAAGCAATCCATTAGTTCA+TGG - chr7_4:20182323-20182342 Msa1144240:CDS 40.0%
! ATGTGCTGACCATGAACTAA+TGG + chr7_4:20182335-20182354 None:intergenic 40.0%
ATGGGGAAAATACTCACGAT+CGG + chr7_4:20182501-20182520 None:intergenic 40.0%
! ATAACTCTGAGGACACCTTT+GGG + chr7_4:20182700-20182719 None:intergenic 40.0%
!! AAAGGTGTCCTCAGAGTTAT+GGG - chr7_4:20182700-20182719 Msa1144240:intron 40.0%
! TGCTAGTCGGTATTTCTGTA+TGG + chr7_4:20182847-20182866 None:intergenic 40.0%
AGACTGACAAGAAAGAAACG+GGG - chr7_4:20183043-20183062 Msa1144240:intron 40.0%
TCACGATGAACCATCTAGAT+TGG + chr7_4:20183079-20183098 None:intergenic 40.0%
ACTAGTGCAAATTTGACTCC+AGG + chr7_4:20183232-20183251 None:intergenic 40.0%
TAATGTTACATGTCTGCAGG+GGG - chr7_4:20183389-20183408 Msa1144240:intron 40.0%
! CGAAGCACTAAAGCTTCAAA+TGG - chr7_4:20183423-20183442 Msa1144240:intron 40.0%
AGCACTAAAGCTTCAAATGG+AGG - chr7_4:20183426-20183445 Msa1144240:intron 40.0%
GAAGCGACTACATGAACAAT+TGG - chr7_4:20183453-20183472 Msa1144240:intron 40.0%
CAGATCAGATCAGTTCAACA+AGG + chr7_4:20183489-20183508 None:intergenic 40.0%
TGCAGAGACAGCTACAATTA+AGG - chr7_4:20183748-20183767 Msa1144240:intron 40.0%
!! GCTCTTTAGCAGCTTTTTCT+GGG + chr7_4:20183932-20183951 None:intergenic 40.0%
!! TGCTCTTTAGCAGCTTTTTC+TGG + chr7_4:20183933-20183952 None:intergenic 40.0%
TTAGTTGATCTCTCGCCATT+AGG + chr7_4:20184053-20184072 None:intergenic 40.0%
TAAAAAGCAACGAGTAAGCG+TGG - chr7_4:20184070-20184089 Msa1144240:intron 40.0%
AGAAGGATCAAAGTGATCTC+GGG + chr7_4:20184189-20184208 None:intergenic 40.0%
CTTGTAGACATGCCTAAAGA+AGG + chr7_4:20184206-20184225 None:intergenic 40.0%
TTAGGCATGTCTACAAGAAG+TGG - chr7_4:20184209-20184228 Msa1144240:intron 40.0%
TACAAGAAGTGGTGAGGAAT+TGG - chr7_4:20184220-20184239 Msa1144240:intron 40.0%
CTACACAGCTCCGAAATTGA+AGG - chr7_4:20180367-20180386 Msa1144240:three_prime_UTR 45.0%
ACACAGCTCCGAAATTGAAG+GGG - chr7_4:20180369-20180388 Msa1144240:three_prime_UTR 45.0%
!!! GGTGACCCTTTTGGCTTATT+TGG - chr7_4:20180571-20180590 Msa1144240:CDS 45.0%
! TTTAGGGTTTAGGGTTTGGG+GGG - chr7_4:20181141-20181160 Msa1144240:intron 45.0%
GACGAAAGGTTACCTATGCT+AGG - chr7_4:20181174-20181193 Msa1144240:intron 45.0%
AGGTTACCTATGCTAGGGAA+AGG - chr7_4:20181180-20181199 Msa1144240:intron 45.0%
! GCTAGCAGCATCAACGATTT+TGG + chr7_4:20181296-20181315 None:intergenic 45.0%
GGTTTGACCTAAGAAGGTCT+TGG + chr7_4:20181520-20181539 None:intergenic 45.0%
TCTTGTATGGGTGGATGGAT+GGG + chr7_4:20181559-20181578 None:intergenic 45.0%
TTCTTGTATGGGTGGATGGA+TGG + chr7_4:20181560-20181579 None:intergenic 45.0%
AACAGTGTGCCTATTCCATG+AGG - chr7_4:20181647-20181666 Msa1144240:intron 45.0%
! TTTAGACCCATGCTTTTGCC+CGG - chr7_4:20181835-20181854 Msa1144240:intron 45.0%
TGCTGTGTGCAGAAAGTCAT+TGG - chr7_4:20181901-20181920 Msa1144240:intron 45.0%
!!! ATGGAGCATTGGTTTTCTGG+GGG - chr7_4:20181950-20181969 Msa1144240:intron 45.0%
ACTCAAAGCAACGTTTGCGT+TGG - chr7_4:20182423-20182442 Msa1144240:CDS 45.0%
! TGAACGCTTTGTTGATGCTG+TGG - chr7_4:20182460-20182479 Msa1144240:CDS 45.0%
TGGGGAAAATACTCACGATC+GGG + chr7_4:20182500-20182519 None:intergenic 45.0%
! CATAACTCTGAGGACACCTT+TGG + chr7_4:20182701-20182720 None:intergenic 45.0%
!! CAAAGGTGTCCTCAGAGTTA+TGG - chr7_4:20182699-20182718 Msa1144240:intron 45.0%
CTTGTACACCCATAACTCTG+AGG + chr7_4:20182711-20182730 None:intergenic 45.0%
CTCAGAGTTATGGGTGTACA+AGG - chr7_4:20182709-20182728 Msa1144240:intron 45.0%
CCTGCAAATGGCTCTTAACA+TGG + chr7_4:20182747-20182766 None:intergenic 45.0%
CCATGTTAAGAGCCATTTGC+AGG - chr7_4:20182744-20182763 Msa1144240:intron 45.0%
GAGTGCACATTACCTGCAAA+TGG + chr7_4:20182759-20182778 None:intergenic 45.0%
TTGCAGGTAATGTGCACTCT+AGG - chr7_4:20182760-20182779 Msa1144240:intron 45.0%
CACTCTAGGTCCTCTTAATG+TGG - chr7_4:20182774-20182793 Msa1144240:intron 45.0%
GACTGACAAGAAAGAAACGG+GGG - chr7_4:20183044-20183063 Msa1144240:intron 45.0%
ATTCAATGCCTCAAACCCGT+TGG - chr7_4:20183286-20183305 Msa1144240:intron 45.0%
!! TTTTTCCACCAACGGGTTTG+AGG + chr7_4:20183297-20183316 None:intergenic 45.0%
GCGACTACATGAACAATTGG+AGG - chr7_4:20183456-20183475 Msa1144240:intron 45.0%
CTTATTGTCGAGCTCCTGTT+GGG + chr7_4:20183889-20183908 None:intergenic 45.0%
TCTTATTGTCGAGCTCCTGT+TGG + chr7_4:20183890-20183909 None:intergenic 45.0%
GCTCGACAATAAGACTGAAC+CGG - chr7_4:20183896-20183915 Msa1144240:intron 45.0%
!! TAGCAGCTTTTTCTGGGTCA+GGG + chr7_4:20183926-20183945 None:intergenic 45.0%
!! TTAGCAGCTTTTTCTGGGTC+AGG + chr7_4:20183927-20183946 None:intergenic 45.0%
TCTGCTGAAAGACTCTTTGC+TGG + chr7_4:20183960-20183979 None:intergenic 45.0%
!! AATCTGGTGTCATCGGTTCA+TGG + chr7_4:20183998-20184017 None:intergenic 45.0%
!! CAACCAGAATCTGGTGTCAT+CGG + chr7_4:20184005-20184024 None:intergenic 45.0%
CTTGACTCCAGTATCTGATG+AGG + chr7_4:20184128-20184147 None:intergenic 45.0%
ATCTCGGGTAAGGAAAACAG+CGG + chr7_4:20184174-20184193 None:intergenic 45.0%
GATCAAAGTGATCTCGGGTA+AGG + chr7_4:20184184-20184203 None:intergenic 45.0%
CATGTCTACAAGAAGTGGTG+AGG - chr7_4:20184214-20184233 Msa1144240:intron 45.0%
GGTGAGGAATTGGATAAGCT+TGG - chr7_4:20184230-20184249 Msa1144240:intron 45.0%
GAGGAATTGGATAAGCTTGG+TGG - chr7_4:20184233-20184252 Msa1144240:intron 45.0%
! AATTGGATAAGCTTGGTGGC+AGG - chr7_4:20184237-20184256 Msa1144240:intron 45.0%
!! TTGAGTTTTTGGGCCCATCT+TGG - chr7_4:20184481-20184500 Msa1144240:five_prime_UTR 45.0%
!! TGAGTTTTTGGGCCCATCTT+GGG - chr7_4:20184482-20184501 Msa1144240:five_prime_UTR 45.0%
! AGCTTTTGTTGATGTGGGGG+AGG - chr7_4:20180757-20180776 Msa1144240:CDS 50.0%
! TTAGGGTTTAGGGTTTGGGG+GGG - chr7_4:20181142-20181161 Msa1144240:intron 50.0%
TTGGGTTCAAACCCGGACAA+AGG + chr7_4:20181501-20181520 None:intergenic 50.0%
GAAGGTCTTGGGTTCAAACC+CGG + chr7_4:20181508-20181527 None:intergenic 50.0%
ATGGGTGGGGGTAGATCAAT+TGG + chr7_4:20181541-20181560 None:intergenic 50.0%
! TTAGACCCATGCTTTTGCCC+GGG - chr7_4:20181836-20181855 Msa1144240:intron 50.0%
CATGGTCAGCACATAGAGTC+TGG - chr7_4:20182341-20182360 Msa1144240:CDS 50.0%
GTCAGCACATAGAGTCTGGT+AGG - chr7_4:20182345-20182364 Msa1144240:CDS 50.0%
AGACTGTTTCCTCCACTACC+AGG + chr7_4:20182383-20182402 None:intergenic 50.0%
GTTGATGCTGTGGCTCAACT+TGG - chr7_4:20182470-20182489 Msa1144240:CDS 50.0%
GGGGAAAATACTCACGATCG+GGG + chr7_4:20182499-20182518 None:intergenic 50.0%
TTACCTGACTGCTCATCCGA+TGG - chr7_4:20182873-20182892 Msa1144240:intron 50.0%
AAGAGCAACAGCGACTCAGT+GGG - chr7_4:20183808-20183827 Msa1144240:intron 50.0%
TGGGACATATCTCCTGGAGT+TGG + chr7_4:20183870-20183889 None:intergenic 50.0%
TCCAGGAGATATGTCCCAAC+AGG - chr7_4:20183872-20183891 Msa1144240:intron 50.0%
TCCTGTTGGGACATATCTCC+TGG + chr7_4:20183876-20183895 None:intergenic 50.0%
!! AGCAGCTTTTTCTGGGTCAG+GGG + chr7_4:20183925-20183944 None:intergenic 50.0%
GAACCGATGACACCAGATTC+TGG - chr7_4:20183999-20184018 Msa1144240:intron 50.0%
! CCAGATTCTGGTTGCCAAGT+CGG - chr7_4:20184011-20184030 Msa1144240:intron 50.0%
TCTCCTGCTGACAGTCCTAA+TGG - chr7_4:20184035-20184054 Msa1144240:intron 50.0%
GGTGCTTCCTCATCAGATAC+TGG - chr7_4:20184118-20184137 Msa1144240:intron 50.0%
! TGATGTGGGGGAGGTTTTGC+TGG - chr7_4:20180766-20180785 Msa1144240:CDS 55.0%
! GATGTGGGGGAGGTTTTGCT+GGG - chr7_4:20180767-20180786 Msa1144240:CDS 55.0%
! TAGGGTTTAGGGTTTGGGGG+GGG - chr7_4:20181143-20181162 Msa1144240:intron 55.0%
TATGGGTGGATGGATGGGTG+GGG + chr7_4:20181554-20181573 None:intergenic 55.0%
GTATGGGTGGATGGATGGGT+GGG + chr7_4:20181555-20181574 None:intergenic 55.0%
TGTATGGGTGGATGGATGGG+TGG + chr7_4:20181556-20181575 None:intergenic 55.0%
! GTGAACCCGGGCAAAAGCAT+GGG + chr7_4:20181844-20181863 None:intergenic 55.0%
AGTGAACCCGGGCAAAAGCA+TGG + chr7_4:20181845-20181864 None:intergenic 55.0%
GAGGGGTGACTCAAACATGC+AGG + chr7_4:20182170-20182189 None:intergenic 55.0%
!! AGAGTCTGGTAGGAGCGCTA+TGG - chr7_4:20182355-20182374 Msa1144240:CDS 55.0%
AGCGCTATGGATCCTGGTAG+TGG - chr7_4:20182368-20182387 Msa1144240:CDS 55.0%
!! GCTATGGATCCTGGTAGTGG+AGG - chr7_4:20182371-20182390 Msa1144240:CDS 55.0%
GATGCTGTGGCTCAACTTGG+TGG - chr7_4:20182473-20182492 Msa1144240:CDS 55.0%
GGGTGTGGCACCTAAGGAAA+AGG + chr7_4:20182680-20182699 None:intergenic 55.0%
! TCCTTAGGTGCCACACCCAA+AGG - chr7_4:20182682-20182701 Msa1144240:intron 55.0%
ACCTTTGGGTGTGGCACCTA+AGG + chr7_4:20182686-20182705 None:intergenic 55.0%
TCTGAGGACACCTTTGGGTG+TGG + chr7_4:20182695-20182714 None:intergenic 55.0%
GTACCATCGGATGAGCAGTC+AGG + chr7_4:20182879-20182898 None:intergenic 55.0%
CAATGCCTCAAACCCGTTGG+TGG - chr7_4:20183289-20183308 Msa1144240:intron 55.0%
GAAGAGCAACAGCGACTCAG+TGG - chr7_4:20183807-20183826 Msa1144240:intron 55.0%
AGAGCAACAGCGACTCAGTG+GGG - chr7_4:20183809-20183828 Msa1144240:intron 55.0%
!! GGGGTTCTTTCAGAAGCACC+CGG - chr7_4:20183828-20183847 Msa1144240:intron 55.0%
!! TGGTGCTGATACACCAGAGC+CGG + chr7_4:20183850-20183869 None:intergenic 55.0%
GGTGTATCAGCACCAACTCC+AGG - chr7_4:20183855-20183874 Msa1144240:intron 55.0%
! TTTTCTGGGTCAGGGGTTGC+CGG + chr7_4:20183918-20183937 None:intergenic 55.0%
!! CCGACTTGGCAACCAGAATC+TGG + chr7_4:20184014-20184033 None:intergenic 55.0%
TGTCAGCAGGAGAACCGACT+TGG + chr7_4:20184028-20184047 None:intergenic 55.0%
TCGCCATTAGGACTGTCAGC+AGG + chr7_4:20184041-20184060 None:intergenic 55.0%
! GCGGTTGGTTGCTGGTATGA+TGG + chr7_4:20184155-20184174 None:intergenic 55.0%
! GGAAAACAGCGGTTGGTTGC+TGG + chr7_4:20184163-20184182 None:intergenic 55.0%
CGGGTAAGGAAAACAGCGGT+TGG + chr7_4:20184170-20184189 None:intergenic 55.0%
! AGGGTTTAGGGTTTGGGGGG+GGG - chr7_4:20181144-20181163 Msa1144240:intron 60.0%
ATGGGTGGATGGATGGGTGG+GGG + chr7_4:20181553-20181572 None:intergenic 60.0%
!! GGTAGGAGCGCTATGGATCC+TGG - chr7_4:20182362-20182381 Msa1144240:CDS 60.0%
!! CTTTCAGAAGCACCCGGCTC+TGG - chr7_4:20183834-20183853 Msa1144240:intron 60.0%
!! GGTGCTGATACACCAGAGCC+GGG + chr7_4:20183849-20183868 None:intergenic 60.0%
! GGGTTTAGGGTTTGGGGGGG+GGG - chr7_4:20181145-20181164 Msa1144240:intron 65.0%
! GGTTTAGGGTTTGGGGGGGG+GGG - chr7_4:20181146-20181165 Msa1144240:intron 65.0%
! GTTTAGGGTTTGGGGGGGGG+GGG - chr7_4:20181147-20181166 Msa1144240:intron 65.0%
! TTTAGGGTTTGGGGGGGGGG+GGG - chr7_4:20181148-20181167 Msa1144240:intron 65.0%
TTAGGGTTTGGGGGGGGGGG+GGG - chr7_4:20181149-20181168 Msa1144240:intron 70.0%
TAGGGTTTGGGGGGGGGGGG+GGG - chr7_4:20181150-20181169 Msa1144240:intron 75.0%
! AGGGTTTGGGGGGGGGGGGG+GGG - chr7_4:20181151-20181170 Msa1144240:intron 80.0%
! GGGTTTGGGGGGGGGGGGGG+GGG - chr7_4:20181152-20181171 Msa1144240:intron 85.0%
! GGGGGGGGGGGGGGGACGAA+AGG - chr7_4:20181160-20181179 Msa1144240:intron 85.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 20180295 20184510 20180295 ID=Msa1144240;Name=Msa1144240
chr7_4 mRNA 20180295 20184510 20180295 ID=Msa1144240-mRNA-1;Parent=Msa1144240;Name=Msa1144240-mRNA-1;_AED=0.06;_eAED=0.06;_QI=153|1|1|1|0.83|0.71|7|242|330
chr7_4 exon 20180295 20181058 20180295 ID=Msa1144240-mRNA-1:exon:4783;Parent=Msa1144240-mRNA-1
chr7_4 exon 20181328 20181397 20181328 ID=Msa1144240-mRNA-1:exon:4782;Parent=Msa1144240-mRNA-1
chr7_4 exon 20181711 20181771 20181711 ID=Msa1144240-mRNA-1:exon:4781;Parent=Msa1144240-mRNA-1
chr7_4 exon 20181911 20181953 20181911 ID=Msa1144240-mRNA-1:exon:4780;Parent=Msa1144240-mRNA-1
chr7_4 exon 20182040 20182116 20182040 ID=Msa1144240-mRNA-1:exon:4779;Parent=Msa1144240-mRNA-1
chr7_4 exon 20182302 20182532 20182302 ID=Msa1144240-mRNA-1:exon:4778;Parent=Msa1144240-mRNA-1
chr7_4 exon 20184369 20184510 20184369 ID=Msa1144240-mRNA-1:exon:4777;Parent=Msa1144240-mRNA-1
chr7_4 five_prime_UTR 20184369 20184510 20184369 ID=Msa1144240-mRNA-1:five_prime_utr;Parent=Msa1144240-mRNA-1
chr7_4 five_prime_UTR 20182522 20182532 20182522 ID=Msa1144240-mRNA-1:five_prime_utr;Parent=Msa1144240-mRNA-1
chr7_4 CDS 20182302 20182521 20182302 ID=Msa1144240-mRNA-1:cds;Parent=Msa1144240-mRNA-1
chr7_4 CDS 20182040 20182116 20182040 ID=Msa1144240-mRNA-1:cds;Parent=Msa1144240-mRNA-1
chr7_4 CDS 20181911 20181953 20181911 ID=Msa1144240-mRNA-1:cds;Parent=Msa1144240-mRNA-1
chr7_4 CDS 20181711 20181771 20181711 ID=Msa1144240-mRNA-1:cds;Parent=Msa1144240-mRNA-1
chr7_4 CDS 20181328 20181397 20181328 ID=Msa1144240-mRNA-1:cds;Parent=Msa1144240-mRNA-1
chr7_4 CDS 20180537 20181058 20180537 ID=Msa1144240-mRNA-1:cds;Parent=Msa1144240-mRNA-1
chr7_4 three_prime_UTR 20180295 20180536 20180295 ID=Msa1144240-mRNA-1:three_prime_utr;Parent=Msa1144240-mRNA-1
Gene Sequence

>Msa1144240

ATGTATCAGCCAACTAATGTTCCTGATTCGAGTTTAATTGGAAGCAATCCATTAGTTCATGGTCAGCACATAGAGTCTGGTAGGAGCGCTATGGATCCTGGTAGTGGAGGAAACAGTCTTGCCAACAACTCTAATCTCAACTCAAAGCAACGTTTGCGTTGGACACATGAGCTACATGAACGCTTTGTTGATGCTGTGGCTCAACTTGGTGGCCCCGATCGTGCCACACCCAAAGGTGTCCTCAGAGTTATGGGTGTACAAGGCTTAACAATTTACCATGTTAAGAGCCATTTGCAGAAATACCGACTAGCAAAATACTTACCTGACTGCTCATCCGATGAGGGGAAAAAGACTGACAAGAAAGAAACGGGGGATATGCTTTCCAATCTAGATGGTTCATCGGGGATGCAGATTACCGAAGCACTAAAGCTTCAAATGGAGGTACAGAAGCGACTACATGAACAATTGGAGGTGCAGAGACAGCTACAATTAAGGATAGAAGCTCAGGGAAAATATTTGAAAAAGATAATCGAAGAGCAACAGCGACTCAGTGGGGTTCTTTCAGAAGCACCCGGCTCTGGTGTATCAGCACCAACTCCAGGAGATATGTCCCAACAGGAGCTCGACAATAAGACTGAACCGGCAACCCCTGACCCAGAAAAAGCTGCTAAAGAGCATGCACCAGCAAAGAGTCTTTCAGCAGAATCTTTTTCATCTCACCATGAACCGATGACACCAGATTCTGGTTGCCAAGTCGGTTCTCCTGCTGACAGTCCTAATGGCGAGAGATCAACTAAAAAGCAACGAGTAAGCGTGGAAGAAGCGTATTTGAAATCAGACATGGTGCTTCCTCATCAGATACTGGAGTCAAGCATGCCATCATACCAGCAACCAACCGCTGTTTTCCTTACCCGAGATCACTTTGATCCTTCTTTAGGCATGTCTACAAGAAGTGGTGAGGAATTGGATAAGCTTGGTGGCAGGAATATGTGA
Protein sequence

>Msa1144240

MYQPTNVPDSSLIGSNPLVHGQHIESGRSAMDPGSGGNSLANNSNLNSKQRLRWTHELHERFVDAVAQLGGPDRATPKGVLRVMGVQGLTIYHVKSHLQKYRLAKYLPDCSSDEGKKTDKKETGDMLSNLDGSSGMQITEALKLQMEVQKRLHEQLEVQRQLQLRIEAQGKYLKKIIEEQQRLSGVLSEAPGSGVSAPTPGDMSQQELDNKTEPATPDPEKAAKEHAPAKSLSAESFSSHHEPMTPDSGCQVGSPADSPNGERSTKKQRVSVEEAYLKSDMVLPHQILESSMPSYQQPTAVFLTRDHFDPSLGMSTRSGEELDKLGGRNM*