AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0239940

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0239940 MtrunA17_Chr2g0291021 97.273 330 9 0 1 330 1 330 0.0 663
Msa0239940 MtrunA17_Chr7g0254981 73.050 141 37 1 49 188 50 190 8.39e-69 216
Msa0239940 MtrunA17_Chr7g0261911 60.390 154 52 3 33 186 252 396 6.97e-53 181
Msa0239940 MtrunA17_Chr1g0175091 48.087 183 77 3 35 217 244 408 1.95e-51 176
Msa0239940 MtrunA17_Chr1g0190301 53.216 171 73 3 33 200 156 322 1.24e-48 167
Msa0239940 MtrunA17_Chr7g0258551 51.351 148 69 2 46 193 209 353 2.99e-47 164
Msa0239940 MtrunA17_Chr7g0274471 53.333 150 68 1 48 197 217 364 1.35e-46 163
Msa0239940 MtrunA17_Chr8g0356501 59.398 133 51 2 49 179 37 168 4.32e-44 154
Msa0239940 MtrunA17_Chr5g0409731 52.448 143 58 1 49 181 25 167 6.24e-44 152
Msa0239940 MtrunA17_Chr4g0042451 44.792 192 80 6 47 220 15 198 2.14e-43 152
Msa0239940 MtrunA17_Chr2g0310011 58.779 131 47 2 49 175 35 162 4.51e-42 149
Msa0239940 MtrunA17_Chr7g0242521 46.497 157 75 4 49 200 13 165 5.75e-41 143
Msa0239940 MtrunA17_Chr5g0417521 44.262 183 87 5 47 217 13 192 7.39e-41 146
Msa0239940 MtrunA17_Chr2g0321461 49.682 157 61 3 47 185 43 199 9.79e-41 146
Msa0239940 MtrunA17_Chr6g0461641 48.701 154 63 2 47 185 40 192 1.19e-40 146
Msa0239940 MtrunA17_Chr2g0290991 47.826 138 56 2 49 173 39 173 2.40e-34 127
Msa0239940 MtrunA17_Chr1g0197171 47.059 136 63 2 49 176 44 178 7.00e-33 123
Msa0239940 MtrunA17_Chr1g0175071 46.043 139 66 2 49 184 177 309 1.08e-30 118
Msa0239940 MtrunA17_Chr1g0175081 48.000 75 39 0 35 109 8 82 2.97e-22 89.7
Msa0239940 MtrunA17_Chr7g0258531 37.222 180 94 5 13 180 171 343 7.52e-22 94.7
Msa0239940 MtrunA17_Chr2g0288601 66.071 56 19 0 51 106 73 128 1.03e-16 75.5
Msa0239940 MtrunA17_Chr7g0243081 61.404 57 22 0 51 107 73 129 1.49e-16 79.3
Msa0239940 MtrunA17_Chr6g0479431 60.000 65 24 1 51 113 204 268 2.58e-16 79.0
Msa0239940 MtrunA17_Chr8g0366281 46.535 101 44 3 51 151 175 265 2.77e-16 78.6
Msa0239940 MtrunA17_Chr3g0095611 48.718 78 24 1 51 112 21 98 7.91e-16 75.1
Msa0239940 MtrunA17_Chr1g0198601 56.061 66 29 0 49 114 211 276 1.48e-15 76.6
Msa0239940 MtrunA17_Chr5g0410741 63.636 55 20 0 51 105 221 275 2.24e-15 76.3
Msa0239940 MtrunA17_Chr8g0379121 63.636 55 20 0 51 105 113 167 2.34e-15 76.6
Msa0239940 MtrunA17_Chr1g0162931 53.425 73 33 1 51 123 20 91 2.89e-15 75.1
Msa0239940 MtrunA17_Chr3g0109981 63.636 55 20 0 51 105 276 330 3.19e-15 76.3
Msa0239940 MtrunA17_Chr2g0320231 65.455 55 19 0 51 105 99 153 4.52e-15 75.5
Msa0239940 MtrunA17_Chr1g0154261 61.818 55 21 0 51 105 128 182 5.88e-15 74.3
Msa0239940 MtrunA17_Chr1g0210911 65.385 52 18 0 51 102 24 75 6.45e-15 74.7
Msa0239940 MtrunA17_Chr5g0402631 55.714 70 30 1 49 117 214 283 4.10e-14 72.4
Msa0239940 MtrunA17_Chr2g0283951 67.347 49 16 0 51 99 91 139 1.46e-13 70.9
Msa0239940 MtrunA17_Chr5g0422621 58.182 55 23 0 49 103 198 252 5.22e-13 68.9
Msa0239940 MtrunA17_Chr4g0062921 48.718 78 39 1 39 115 224 301 6.08e-13 69.3
Msa0239940 MtrunA17_Chr4g0009981 50.685 73 33 2 41 113 108 177 1.01e-12 67.4
Msa0239940 MtrunA17_Chr4g0045361 60.000 55 22 0 49 103 274 328 1.17e-12 68.2
Msa0239940 MtrunA17_Chr4g0009901 56.364 55 23 1 49 103 168 221 1.98e-12 66.6
Msa0239940 MtrunA17_Chr4g0031961 48.611 72 35 2 49 119 131 201 7.83e-12 65.1
Msa0239940 MtrunA17_Chr2g0324891 50.820 61 29 1 42 102 154 213 9.01e-12 65.5
Msa0239940 MtrunA17_Chr5g0400241 43.038 79 44 1 27 105 289 366 1.27e-11 65.5
Msa0239940 MtrunA17_Chr7g0255711 58.182 55 22 1 49 103 91 144 1.33e-11 64.3
Msa0239940 MtrunA17_Chr4g0009851 52.308 65 28 2 49 113 169 230 2.08e-11 63.9
Msa0239940 MtrunA17_Chr2g0304841 54.545 55 24 1 49 103 213 266 2.56e-11 64.7
Msa0239940 MtrunA17_Chr4g0067981 58.182 55 22 1 49 103 215 268 3.46e-11 64.3
Msa0239940 MtrunA17_Chr4g0040481 53.704 54 24 1 49 102 158 210 4.84e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0239940 AT2G01060.1 66.779 298 69 9 43 330 9 286 3.04e-119 345
Msa0239940 AT2G01060.2 62.646 257 66 9 84 330 1 237 1.80e-88 265
Msa0239940 AT5G29000.1 57.692 156 63 2 29 183 170 323 2.09e-51 174
Msa0239940 AT5G29000.3 57.692 156 63 2 29 183 170 323 2.09e-51 174
Msa0239940 AT5G29000.2 57.692 156 63 2 29 183 213 366 4.06e-51 175
Msa0239940 AT3G04450.4 52.273 176 76 3 44 212 193 367 7.56e-51 174
Msa0239940 AT3G04450.2 52.273 176 76 3 44 212 193 367 7.56e-51 174
Msa0239940 AT3G04450.1 52.273 176 76 3 44 212 233 407 1.34e-50 174
Msa0239940 AT4G28610.1 52.432 185 80 4 41 225 217 393 4.26e-50 172
Msa0239940 AT5G06800.1 57.534 146 57 3 37 182 182 322 6.49e-50 171
Msa0239940 AT4G13640.1 59.574 141 47 2 49 179 37 177 1.91e-49 167
Msa0239940 AT3G13040.3 50.265 189 86 4 44 227 236 421 1.98e-49 171
Msa0239940 AT3G13040.1 50.265 189 86 4 44 227 236 421 1.98e-49 171
Msa0239940 AT3G13040.2 50.265 189 86 4 44 227 236 421 1.98e-49 171
Msa0239940 AT3G24120.1 58.156 141 49 2 49 179 41 181 7.71e-49 166
Msa0239940 AT5G06800.2 55.705 149 60 3 37 182 182 327 7.97e-49 168
Msa0239940 AT1G79430.2 61.268 142 47 3 49 185 34 172 2.76e-48 166
Msa0239940 AT4G13640.2 58.333 144 47 3 49 179 37 180 3.08e-47 161
Msa0239940 AT3G24120.2 56.944 144 49 3 49 179 41 184 9.96e-47 160
Msa0239940 AT3G04030.3 48.333 180 72 4 23 185 19 194 8.16e-43 152
Msa0239940 AT1G69580.2 49.693 163 65 3 47 192 32 194 9.30e-43 151
Msa0239940 AT2G20400.2 53.165 158 71 2 49 206 231 385 1.04e-42 152
Msa0239940 AT1G69580.1 49.091 165 62 4 47 192 32 193 1.05e-42 150
Msa0239940 AT2G20400.1 53.797 158 69 2 49 206 231 384 1.33e-42 152
Msa0239940 AT5G18240.1 51.316 152 61 1 47 185 43 194 4.04e-42 150
Msa0239940 AT5G18240.4 51.316 152 61 1 47 185 43 194 4.04e-42 150
Msa0239940 AT3G04030.2 47.778 180 72 5 23 185 19 193 3.73e-40 145
Msa0239940 AT5G18240.5 50.000 152 61 2 47 185 43 192 7.33e-39 142
Msa0239940 AT5G45580.1 45.223 157 56 3 49 182 7 156 4.34e-37 134
Msa0239940 AT3G04030.1 45.556 180 71 5 23 185 19 188 6.82e-37 136
Msa0239940 AT5G45580.2 45.860 157 55 3 49 182 23 172 8.00e-37 134
Msa0239940 AT5G18240.2 48.026 152 60 2 47 185 43 188 3.20e-36 135
Msa0239940 AT5G18240.3 48.026 152 60 2 47 185 43 188 3.20e-36 135
Msa0239940 AT3G12730.1 47.826 138 64 4 49 179 23 159 3.09e-35 128
Msa0239940 AT1G79430.1 54.545 110 42 3 81 185 1 107 4.73e-27 108
Msa0239940 AT5G29000.4 60.674 89 33 1 29 117 213 299 3.09e-25 103
Msa0239940 AT3G04450.3 64.286 70 25 0 44 113 233 302 3.48e-25 103
Msa0239940 AT3G04030.5 49.153 118 47 2 81 185 1 118 1.34e-24 102
Msa0239940 AT5G06800.3 60.563 71 26 1 45 115 188 256 5.54e-24 99.8
Msa0239940 AT3G04030.4 44.915 118 46 3 81 185 1 112 4.54e-19 86.7
Msa0239940 AT5G18240.6 43.220 118 48 2 81 185 1 112 5.52e-19 86.7
Msa0239940 AT2G06020.1 59.649 57 23 0 49 105 86 142 7.10e-18 83.2
Msa0239940 AT2G42660.1 65.517 58 20 0 51 108 52 109 2.94e-17 80.5
Msa0239940 AT2G40260.1 57.333 75 20 1 51 125 84 146 3.08e-17 82.4
Msa0239940 AT4G04605.1 69.231 52 16 0 51 102 16 67 4.16e-17 79.3
Msa0239940 AT1G32240.1 43.396 106 51 2 51 156 215 311 1.79e-16 80.1
Msa0239940 AT1G32240.2 43.396 106 51 2 51 156 215 311 1.99e-16 79.7
Msa0239940 AT5G42630.2 57.576 66 28 0 51 116 107 172 4.46e-16 76.6
Msa0239940 AT5G42630.1 57.576 66 28 0 51 116 107 172 5.78e-16 77.4
Msa0239940 AT3G12730.2 39.623 106 56 4 81 179 1 105 1.29e-15 74.3
Msa0239940 AT5G16560.1 60.000 65 24 1 51 113 221 285 1.62e-15 77.4
Msa0239940 AT1G14600.1 69.231 52 16 0 51 102 25 76 2.03e-15 75.5
Msa0239940 AT4G17695.1 63.636 55 20 0 51 105 166 220 3.12e-15 75.9
Msa0239940 AT2G02060.2 69.231 52 16 0 51 102 31 82 3.28e-15 74.7
Msa0239940 AT2G02060.1 69.231 52 16 0 51 102 31 82 3.28e-15 74.7
Msa0239940 AT2G38300.1 63.636 55 20 0 51 105 56 110 3.76e-15 75.9
Msa0239940 AT4G37180.1 60.000 55 22 0 49 103 210 264 3.44e-14 73.2
Msa0239940 AT4G37180.2 60.000 55 22 0 49 103 217 271 4.20e-14 72.8
Msa0239940 AT4G04580.1 54.545 55 25 0 51 105 16 70 1.20e-13 68.6
Msa0239940 AT1G49560.1 54.545 66 30 0 49 114 192 257 1.52e-13 70.9
Msa0239940 AT3G25790.1 58.182 55 23 0 54 108 201 255 2.98e-13 70.1
Msa0239940 AT1G25550.1 55.385 65 29 0 44 108 204 268 5.27e-13 69.3
Msa0239940 AT3G10760.1 48.101 79 40 1 25 103 81 158 7.96e-13 68.9
Msa0239940 AT1G13300.1 44.086 93 41 2 16 108 159 240 1.40e-12 68.2
Msa0239940 AT1G68670.1 48.052 77 40 0 42 118 208 284 1.82e-12 67.8
Msa0239940 AT5G05090.1 60.377 53 20 1 51 103 83 134 4.25e-12 65.9
Msa0239940 AT4G37180.3 55.172 58 26 0 49 106 210 267 6.17e-12 65.5
Msa0239940 AT5G58080.1 52.174 69 28 3 49 117 194 257 7.07e-12 66.6
Msa0239940 AT5G59570.2 58.491 53 21 1 51 103 143 194 9.86e-12 65.1
Msa0239940 AT5G59570.1 58.491 53 21 1 51 103 143 194 9.86e-12 65.1
Msa0239940 AT3G46640.2 58.491 53 21 1 51 103 146 197 1.21e-11 65.1
Msa0239940 AT3G46640.1 58.491 53 21 1 51 103 146 197 1.21e-11 65.1
Msa0239940 AT3G46640.3 58.491 53 21 1 51 103 146 197 1.24e-11 65.1
Msa0239940 AT2G20570.1 51.471 68 31 2 35 102 140 205 1.35e-11 65.5
Msa0239940 AT2G03500.1 53.226 62 29 0 47 108 231 292 1.63e-11 65.5
Msa0239940 AT2G01760.3 45.333 75 38 2 49 123 135 206 1.66e-11 64.7
Msa0239940 AT5G44190.1 46.154 65 34 1 38 102 136 199 2.79e-11 64.3
Msa0239940 AT2G01760.1 45.333 75 38 2 49 123 200 271 2.96e-11 64.3
Msa0239940 AT5G44190.2 46.154 65 34 1 38 102 136 199 3.20e-11 63.9
Msa0239940 AT2G01760.2 45.333 75 38 2 49 123 228 299 4.22e-11 63.9
Msa0239940 AT2G40970.1 56.364 55 23 1 49 103 105 158 4.60e-11 62.8
Msa0239940 AT3G25790.2 58.696 46 19 0 54 99 201 246 9.79e-11 62.0

Find 99 sgRNAs with CRISPR-Local

Find 298 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCCTGATTCGAGTTTAAT+TGG 0.223090 2_2:+14552889 Msa0239940:CDS
CTCTAAAAGTAATTAAATTA+AGG 0.260834 2_2:-14554987 None:intergenic
GATCACTTTGATCCTTCTTT+AGG 0.297515 2_2:+14554778 Msa0239940:CDS
TTTAGAGAACTGATTTGAAT+TGG 0.300478 2_2:+14555003 Msa0239940:three_prime_UTR
TTAGTTGATCGCTCGCCATT+AGG 0.329748 2_2:-14554637 None:intergenic
CCATGTTAAGAGCCATTTGC+AGG 0.349218 2_2:+14553331 Msa0239940:CDS
TACAAGAAGTGGTGAGGAAT+TGG 0.349818 2_2:+14554807 Msa0239940:CDS
TGCAGAGACAGCTACAATTA+AGG 0.349960 2_2:+14554335 Msa0239940:CDS
TGGATTTCATAGATCTCATC+TGG 0.363640 2_2:+14551005 Msa0239940:five_prime_UTR
CTTCCAATTAAACTCGAATC+AGG 0.368180 2_2:-14552892 None:intergenic
CATAACTCTGAGGACACCTT+TGG 0.375653 2_2:-14553285 None:intergenic
CAAAGGTGTCCTCAGAGTTA+TGG 0.377183 2_2:+14553286 Msa0239940:CDS
ATGTGCTGACCATGAACTAA+TGG 0.385679 2_2:-14552919 None:intergenic
GAACCGATGACACCAGATTC+TGG 0.386395 2_2:+14554586 Msa0239940:CDS
AAGACTGACAAGAAAGAAAC+GGG 0.390608 2_2:+14553629 Msa0239940:CDS
TGAGTTGAGATTAGAGTTGT+TGG 0.390955 2_2:-14552992 None:intergenic
GGAAAACAGCGGTTGGTTGC+TGG 0.394253 2_2:-14554747 None:intergenic
AAAGGTGTCCTCAGAGTTAT+GGG 0.402741 2_2:+14553287 Msa0239940:CDS
ACAATTAAGGATAGAAGCTC+AGG 0.410929 2_2:+14554348 Msa0239940:CDS
GGAAGCAATCCATTAGTTCA+TGG 0.415844 2_2:+14552910 Msa0239940:CDS
TTAGAGAACTGATTTGAATT+GGG 0.421756 2_2:+14555004 Msa0239940:three_prime_UTR
CATTTGAAGCTTTAGTGCTT+CGG 0.424328 2_2:-14554009 None:intergenic
GATATGCTTTCCAATCTAGA+TGG 0.424452 2_2:+14553653 Msa0239940:CDS
TCCTGTTGGGACATATCTCC+TGG 0.428112 2_2:-14554460 None:intergenic
TTATATGCATTCTTAGTTGA+TGG 0.434188 2_2:+14554896 Msa0239940:three_prime_UTR
TCTGCTGAAAGACTCTTTGC+TGG 0.439579 2_2:-14554544 None:intergenic
GGTGCTTCCTCATCAGATAC+TGG 0.444138 2_2:+14554705 Msa0239940:CDS
CTTTCAGAAGCACCCGGCTC+TGG 0.447624 2_2:+14554421 Msa0239940:CDS
CCGACTTGGCAACCAGAATC+TGG 0.448662 2_2:-14554598 None:intergenic
CTCGAATCAGGAACATTAGT+TGG 0.450860 2_2:-14552880 None:intergenic
TCTCCTGCTGACAGTCCTAA+TGG 0.454643 2_2:+14554622 Msa0239940:CDS
TCACGATGAACCATCTAGAT+TGG 0.455940 2_2:-14553663 None:intergenic
GTTGATGCTGTGGCTCAACT+TGG 0.466308 2_2:+14553057 Msa0239940:CDS
AAGAAGGATCAAAGTGATCT+CGG 0.468691 2_2:-14554774 None:intergenic
GAAGCGACTACATGAACAAT+TGG 0.469698 2_2:+14554040 Msa0239940:CDS
CGAAGCACTAAAGCTTCAAA+TGG 0.471790 2_2:+14554010 Msa0239940:CDS
TCCAGGAGATATGTCCCAAC+AGG 0.476349 2_2:+14554459 Msa0239940:CDS
ATAACTCTGAGGACACCTTT+GGG 0.476739 2_2:-14553284 None:intergenic
ATTAAATTGGTAGCCCAAGA+TGG 0.479332 2_2:-14555082 None:intergenic
TCTTATTGTCGAGCTCCTGT+TGG 0.481895 2_2:-14554474 None:intergenic
CTTATTGTCGAGCTCCTGTT+GGG 0.485642 2_2:-14554473 None:intergenic
CTCAGAGTTATGGGTGTACA+AGG 0.485843 2_2:+14553296 Msa0239940:CDS
GGTAGGAGCGCTATGGATCC+TGG 0.493466 2_2:+14552949 Msa0239940:CDS
CTACACAGCTCCGAAATTGA+AGG 0.498072 2_2:+14550958 Msa0239940:five_prime_UTR
CATGGTCAGCACATAGAGTC+TGG 0.500025 2_2:+14552928 Msa0239940:CDS
GAGTGCACATTACCTGCAAA+TGG 0.500488 2_2:-14553343 None:intergenic
AAAGACTGACAAGAAAGAAA+CGG 0.503951 2_2:+14553628 Msa0239940:CDS
TCCTTAGGTGCCACACCCAA+AGG 0.508503 2_2:+14553269 Msa0239940:intron
GATGCTGTGGCTCAACTTGG+TGG 0.512420 2_2:+14553060 Msa0239940:CDS
GCTCGACAATAAGACTGAAC+CGG 0.517717 2_2:+14554483 Msa0239940:CDS
TGGTGCTGATACACCAGAGC+CGG 0.522258 2_2:-14554434 None:intergenic
AATCTGGTGTCATCGGTTCA+TGG 0.523061 2_2:-14554582 None:intergenic
GATCAAAGTGATCTCGGGTA+AGG 0.525714 2_2:-14554768 None:intergenic
CCTGCAAATGGCTCTTAACA+TGG 0.525821 2_2:-14553331 None:intergenic
CCAGATTCTGGTTGCCAAGT+CGG 0.528854 2_2:+14554598 Msa0239940:CDS
GTACCATCGGATGAGCAGTC+AGG 0.531157 2_2:-14553463 None:intergenic
ACTCAAAGCAACGTTTGCGT+TGG 0.531602 2_2:+14553010 Msa0239940:CDS
AGAAGGATCAAAGTGATCTC+GGG 0.531953 2_2:-14554773 None:intergenic
GGTGTATCAGCACCAACTCC+AGG 0.533448 2_2:+14554442 Msa0239940:CDS
AGCGCTATGGATCCTGGTAG+TGG 0.534994 2_2:+14552955 Msa0239940:CDS
CTTGTAGACATGCCTAAAGA+AGG 0.541592 2_2:-14554790 None:intergenic
TTAGGCATGTCTACAAGAAG+TGG 0.546196 2_2:+14554796 Msa0239940:CDS
CGGGTAAGGAAAACAGCGGT+TGG 0.546421 2_2:-14554754 None:intergenic
GGTGCTGATACACCAGAGCC+GGG 0.552630 2_2:-14554433 None:intergenic
TCTGAGGACACCTTTGGGTG+TGG 0.554653 2_2:-14553279 None:intergenic
CAACCAGAATCTGGTGTCAT+CGG 0.558599 2_2:-14554589 None:intergenic
AGAGTCTGGTAGGAGCGCTA+TGG 0.562642 2_2:+14552942 Msa0239940:CDS
GCGGTTGGTTGCTGGTATGA+TGG 0.564870 2_2:-14554739 None:intergenic
TGTCAGCAGGAGAACCGACT+TGG 0.567410 2_2:-14554612 None:intergenic
AATTGGATAAGCTTGGTGGC+AGG 0.568213 2_2:+14554824 Msa0239940:CDS
TGGGACATATCTCCTGGAGT+TGG 0.568536 2_2:-14554454 None:intergenic
TTAAATTGGTAGCCCAAGAT+GGG 0.568543 2_2:-14555081 None:intergenic
AGACTGTTTCCTCCACTACC+AGG 0.568775 2_2:-14552967 None:intergenic
AAGAGCAACAGCGACTCAGT+GGG 0.575024 2_2:+14554395 Msa0239940:CDS
TACACAGCTCCGAAATTGAA+GGG 0.581590 2_2:+14550959 Msa0239940:five_prime_UTR
TATGCATTCTTAGTTGATGG+AGG 0.586098 2_2:+14554899 Msa0239940:three_prime_UTR
TCGCCATTAGGACTGTCAGC+AGG 0.590479 2_2:-14554625 None:intergenic
GGTGAGGAATTGGATAAGCT+TGG 0.592014 2_2:+14554817 Msa0239940:CDS
TTACCTGACTGCTCATCCGA+TGG 0.595863 2_2:+14553460 Msa0239940:CDS
GCGACTACATGAACAATTGG+AGG 0.598747 2_2:+14554043 Msa0239940:CDS
TGAACGCTTTGTTGATGCTG+TGG 0.601507 2_2:+14553047 Msa0239940:CDS
GGGGTTCTTTCAGAAGCACC+CGG 0.619430 2_2:+14554415 Msa0239940:CDS
GGATTTCATAGATCTCATCT+GGG 0.628659 2_2:+14551006 Msa0239940:five_prime_UTR
GTCAGCACATAGAGTCTGGT+AGG 0.630464 2_2:+14552932 Msa0239940:CDS
AGCACTAAAGCTTCAAATGG+AGG 0.636135 2_2:+14554013 Msa0239940:CDS
GAAGAGCAACAGCGACTCAG+TGG 0.642615 2_2:+14554394 Msa0239940:CDS
CTTGACTCCAGTATCTGATG+AGG 0.648174 2_2:-14554712 None:intergenic
CATGTCTACAAGAAGTGGTG+AGG 0.650625 2_2:+14554801 Msa0239940:CDS
GAGGAATTGGATAAGCTTGG+TGG 0.651592 2_2:+14554820 Msa0239940:CDS
CAATTAAGGATAGAAGCTCA+GGG 0.654933 2_2:+14554349 Msa0239940:CDS
GGGGAAAATACTCACGATCG+GGG 0.658151 2_2:-14553083 None:intergenic
GCTATGGATCCTGGTAGTGG+AGG 0.662575 2_2:+14552958 Msa0239940:CDS
ACACAGCTCCGAAATTGAAG+GGG 0.667923 2_2:+14550960 Msa0239940:five_prime_UTR
GACTGACAAGAAAGAAACGG+GGG 0.678708 2_2:+14553631 Msa0239940:CDS
AGCGTATTTGAAATCAGACA+TGG 0.689566 2_2:+14554684 Msa0239940:CDS
AGAGCAACAGCGACTCAGTG+GGG 0.711050 2_2:+14554396 Msa0239940:CDS
CTTGTACACCCATAACTCTG+AGG 0.715783 2_2:-14553295 None:intergenic
ATCTCGGGTAAGGAAAACAG+CGG 0.741797 2_2:-14554758 None:intergenic
AGACTGACAAGAAAGAAACG+GGG 0.753105 2_2:+14553630 Msa0239940:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAAATAAATAAATGTTAAT+TGG + chr2_2:14551433-14551452 Msa0239940:intron 10.0%
!!! TTTTATTAATTTCAATCAAA+TGG + chr2_2:14552518-14552537 Msa0239940:intron 10.0%
!!! TTGTATTGTTTTTACATTTT+TGG + chr2_2:14551141-14551160 Msa0239940:intron 15.0%
!!! AAGTTTGATTTTTTAAGTTT+TGG + chr2_2:14551229-14551248 Msa0239940:intron 15.0%
!! ATACAATATTAACAACAATT+AGG - chr2_2:14551408-14551427 None:intergenic 15.0%
!! ATAAATAAATGTTAATTGGT+TGG + chr2_2:14551437-14551456 Msa0239940:intron 15.0%
!! ATTAAATTTATGATCTATTG+TGG + chr2_2:14552329-14552348 Msa0239940:intron 15.0%
!!! GATTTAAAAACTTTTAGATA+AGG - chr2_2:14553236-14553255 None:intergenic 15.0%
!! CATTAAAAAAACAAGAATAA+TGG - chr2_2:14553390-14553409 None:intergenic 15.0%
!!! TAAAAAGTTATGTTTCTTTT+AGG + chr2_2:14553849-14553868 Msa0239940:intron 15.0%
!!! TTTTATTTTGAATCAAGTAA+AGG + chr2_2:14554238-14554257 Msa0239940:intron 15.0%
!!! CTCTAAAAGTAATTAAATTA+AGG - chr2_2:14554990-14555009 None:intergenic 15.0%
!!! AAAGCATTCATTGATTTTTA+GGG + chr2_2:14551536-14551555 Msa0239940:intron 20.0%
!! ATCACTAAGATAACATATAT+AGG - chr2_2:14551664-14551683 None:intergenic 20.0%
!!! AGTAATTTGATGATATTTGA+AGG + chr2_2:14551824-14551843 Msa0239940:intron 20.0%
!!! GTAATTTGATGATATTTGAA+GGG + chr2_2:14551825-14551844 Msa0239940:intron 20.0%
!!! TAATTTGATGATATTTGAAG+GGG + chr2_2:14551826-14551845 Msa0239940:intron 20.0%
!!! CTTTTTTTAATGACAAATGT+TGG + chr2_2:14552035-14552054 Msa0239940:intron 20.0%
!!! ACAGTTTGAAAAGTTTTTTA+TGG + chr2_2:14552624-14552643 Msa0239940:intron 20.0%
!! AAATCAGTAAGATCTAAATA+TGG - chr2_2:14553512-14553531 None:intergenic 20.0%
!! AGAATGCTTAATACTTTAAA+CGG - chr2_2:14553547-14553566 None:intergenic 20.0%
!! AAAGTTATCAGCAAATTTAA+AGG - chr2_2:14553586-14553605 None:intergenic 20.0%
!!! ACTTTTTAATTGTCTTATAG+AGG + chr2_2:14553601-14553620 Msa0239940:intron 20.0%
!!! CTTTTTAATTGTCTTATAGA+GGG + chr2_2:14553602-14553621 Msa0239940:intron 20.0%
!!! TTTTTAATTGTCTTATAGAG+GGG + chr2_2:14553603-14553622 Msa0239940:intron 20.0%
!! TATCATAATTATACAAGGAA+TGG + chr2_2:14553713-14553732 Msa0239940:intron 20.0%
!! TAGAAAAAATAACAGTTACA+AGG - chr2_2:14554304-14554323 None:intergenic 20.0%
!!! TTATTTTTTCTATGTGAAAC+AGG + chr2_2:14554312-14554331 Msa0239940:intron 20.0%
!! GAAAATTTTGTATACGTAGT+TGG + chr2_2:14550985-14551004 Msa0239940:five_prime_UTR 25.0%
! TTATAGAAATGAAGAAGAGA+AGG - chr2_2:14551076-14551095 None:intergenic 25.0%
! GAGAAAGTAAGGAATAAAAA+GGG - chr2_2:14551099-14551118 None:intergenic 25.0%
! AGAGAAAGTAAGGAATAAAA+AGG - chr2_2:14551100-14551119 None:intergenic 25.0%
! TAAAACCAAATAAGCCAAAA+GGG - chr2_2:14551170-14551189 None:intergenic 25.0%
! GCTTTCAAAAAGTTAAAACA+AGG - chr2_2:14551521-14551540 None:intergenic 25.0%
!!! GAAAGCATTCATTGATTTTT+AGG + chr2_2:14551535-14551554 Msa0239940:intron 25.0%
! GATGTTAATGTGATGAAAAA+TGG + chr2_2:14551577-14551596 Msa0239940:intron 25.0%
! TGTTATCTTAGTGATTAATC+AGG + chr2_2:14551669-14551688 Msa0239940:intron 25.0%
!!! ATTTTCAACTCCACTTTTTA+GGG + chr2_2:14551716-14551735 Msa0239940:intron 25.0%
! TAGCAAAGAATCTATCTAAT+AGG - chr2_2:14551853-14551872 None:intergenic 25.0%
! AAATTGAATTCAACTTTCTG+TGG + chr2_2:14551932-14551951 Msa0239940:intron 25.0%
!! TAGTTTAAGTTTCTTAGACA+TGG + chr2_2:14552210-14552229 Msa0239940:intron 25.0%
!! GTGTTTGTTAAATACTGTAT+TGG + chr2_2:14552559-14552578 Msa0239940:intron 25.0%
! CGTTGAAAAAAATTGTACAA+CGG - chr2_2:14552604-14552623 None:intergenic 25.0%
! AACTGAATCTGATTAAGAAT+TGG + chr2_2:14552687-14552706 Msa0239940:intron 25.0%
! ATTTCAGCTAACGAAAAAAT+GGG - chr2_2:14553106-14553125 None:intergenic 25.0%
! TTGGTTACATCATACATATT+TGG + chr2_2:14553139-14553158 Msa0239940:intron 25.0%
!! CAGATATCAAATACTTTTGT+TGG + chr2_2:14553164-14553183 Msa0239940:intron 25.0%
! TAAATTCATCATCTAGCATT+AGG - chr2_2:14553198-14553217 None:intergenic 25.0%
! TGGGTTATCATAATTATACA+AGG + chr2_2:14553708-14553727 Msa0239940:intron 25.0%
!!! TATAGATTTTTTTCCACCAA+CGG - chr2_2:14553892-14553911 None:intergenic 25.0%
! TTATATGCATTCTTAGTTGA+TGG + chr2_2:14554896-14554915 Msa0239940:three_prime_UTR 25.0%
!!! TTTAGAGAACTGATTTGAAT+TGG + chr2_2:14555003-14555022 Msa0239940:three_prime_UTR 25.0%
!!! TTAGAGAACTGATTTGAATT+GGG + chr2_2:14555004-14555023 Msa0239940:three_prime_UTR 25.0%
! AAAAACTCAACCTTGAAAAT+GGG - chr2_2:14555058-14555077 None:intergenic 25.0%
!!! ATTTTCAAGGTTGAGTTTTT+GGG + chr2_2:14555058-14555077 Msa0239940:three_prime_UTR 25.0%
TGAAAGAAAGAGAGAAAGTA+AGG - chr2_2:14551110-14551129 None:intergenic 30.0%
CTAAAACCAAATAAGCCAAA+AGG - chr2_2:14551171-14551190 None:intergenic 30.0%
! TTTTCAGCTTTTGTTGATGT+GGG + chr2_2:14551343-14551362 Msa0239940:intron 30.0%
ATAGCTAACATCAGTGAATT+GGG - chr2_2:14551620-14551639 None:intergenic 30.0%
TATAGCTAACATCAGTGAAT+TGG - chr2_2:14551621-14551640 None:intergenic 30.0%
TTCACTGATGTTAGCTATAA+AGG + chr2_2:14551622-14551641 Msa0239940:intron 30.0%
!!! GATTTTCAACTCCACTTTTT+AGG + chr2_2:14551715-14551734 Msa0239940:intron 30.0%
TATCTAGAGATAGATGATGA+TGG - chr2_2:14551910-14551929 None:intergenic 30.0%
!!! TTGATTTTTTCTCCTTTGTC+CGG + chr2_2:14552073-14552092 Msa0239940:intron 30.0%
ACTTGGTAAACTTCTTGTAT+GGG - chr2_2:14552158-14552177 None:intergenic 30.0%
AACTTGGTAAACTTCTTGTA+TGG - chr2_2:14552159-14552178 None:intergenic 30.0%
TTCTAAGTCAAACTAGAACT+TGG - chr2_2:14552175-14552194 None:intergenic 30.0%
!! AGTTCTAGTTTGACTTAGAA+TGG + chr2_2:14552175-14552194 Msa0239940:intron 30.0%
AAACTATTATTAGTGAACCC+GGG - chr2_2:14552443-14552462 None:intergenic 30.0%
CAAACTATTATTAGTGAACC+CGG - chr2_2:14552444-14552463 None:intergenic 30.0%
ATTTCAATCAAATGGAGCAT+TGG + chr2_2:14552526-14552545 Msa0239940:intron 30.0%
AAATTGTACAACGGATCATT+TGG - chr2_2:14552595-14552614 None:intergenic 30.0%
AACTCTCTTTCTTTCAATCA+TGG - chr2_2:14552666-14552685 None:intergenic 30.0%
ATTGGAATTAGAACATGTTG+AGG + chr2_2:14552705-14552724 Msa0239940:intron 30.0%
TTGGAATTAGAACATGTTGA+GGG + chr2_2:14552706-14552725 Msa0239940:intron 30.0%
AGACAGAAACACACAAAAAT+TGG - chr2_2:14552733-14552752 None:intergenic 30.0%
!! AAAGGTTGGTAAAGAAATGA+GGG - chr2_2:14552775-14552794 None:intergenic 30.0%
!! AAAAGGTTGGTAAAGAAATG+AGG - chr2_2:14552776-14552795 None:intergenic 30.0%
ACTTAATTGCAGATCGTAAA+AGG - chr2_2:14552793-14552812 None:intergenic 30.0%
TTTCAGCTAACGAAAAAATG+GGG - chr2_2:14553105-14553124 None:intergenic 30.0%
CATTTCAGCTAACGAAAAAA+TGG - chr2_2:14553107-14553126 None:intergenic 30.0%
TGAAATGTAATGCGTAGAAT+TGG + chr2_2:14553120-14553139 Msa0239940:intron 30.0%
TGCTAGATGATGAATTTATC+AGG + chr2_2:14553200-14553219 Msa0239940:intron 30.0%
! TCTATACTATCCTTTTCCTT+AGG + chr2_2:14553254-14553273 Msa0239940:intron 30.0%
!! ATAATGGATACCACATTAAG+AGG - chr2_2:14553374-14553393 None:intergenic 30.0%
ATTTAAAGGAGGAAACAAAG+TGG - chr2_2:14553572-14553591 None:intergenic 30.0%
GTTATCAGCAAATTTAAAGG+AGG - chr2_2:14553583-14553602 None:intergenic 30.0%
AAAGACTGACAAGAAAGAAA+CGG + chr2_2:14553628-14553647 Msa0239940:CDS 30.0%
TGATAACCCAAAAGCAAAAA+GGG - chr2_2:14553698-14553717 None:intergenic 30.0%
ATGATAACCCAAAAGCAAAA+AGG - chr2_2:14553699-14553718 None:intergenic 30.0%
CTAAATAGAACCATCTCATT+TGG + chr2_2:14553785-14553804 Msa0239940:intron 30.0%
!!! ATAGATTTTTTTCCACCAAC+GGG - chr2_2:14553891-14553910 None:intergenic 30.0%
TAATAATGTTACATGTCTGC+AGG + chr2_2:14553973-14553992 Msa0239940:intron 30.0%
AATAATGTTACATGTCTGCA+GGG + chr2_2:14553974-14553993 Msa0239940:intron 30.0%
GTGAGATGAAATGATGATAT+TGG + chr2_2:14554153-14554172 Msa0239940:intron 30.0%
AAGGAATAATAGACAGAATG+AGG + chr2_2:14554257-14554276 Msa0239940:intron 30.0%
AGGAATAATAGACAGAATGA+GGG + chr2_2:14554258-14554277 Msa0239940:intron 30.0%
CAAAAACTCAACCTTGAAAA+TGG - chr2_2:14555059-14555078 None:intergenic 30.0%
!! CATTTTCAAGGTTGAGTTTT+TGG + chr2_2:14555057-14555076 Msa0239940:three_prime_UTR 30.0%
! ATTTTCGTCCCCTTCAATTT+CGG - chr2_2:14550971-14550990 None:intergenic 35.0%
TGGATTTCATAGATCTCATC+TGG + chr2_2:14551005-14551024 Msa0239940:five_prime_UTR 35.0%
GGATTTCATAGATCTCATCT+GGG + chr2_2:14551006-14551025 Msa0239940:five_prime_UTR 35.0%
!!! TACATTTTTGGTGACCCTTT+TGG + chr2_2:14551153-14551172 Msa0239940:intron 35.0%
AGCACAAAACTACACAAGAA+GGG - chr2_2:14551275-14551294 None:intergenic 35.0%
! CTTTTCAGCTTTTGTTGATG+TGG + chr2_2:14551342-14551361 Msa0239940:intron 35.0%
! TTTCAGCTTTTGTTGATGTG+GGG + chr2_2:14551344-14551363 Msa0239940:intron 35.0%
AAAACCCATAACACTCATCA+TGG - chr2_2:14551493-14551512 None:intergenic 35.0%
ACTGATGTTAGCTATAAAGG+TGG + chr2_2:14551625-14551644 Msa0239940:intron 35.0%
!!! AACTCCACTTTTTAGGGTTT+GGG + chr2_2:14551722-14551741 Msa0239940:intron 35.0%
!!! TGATTTTTTCTCCTTTGTCC+GGG + chr2_2:14552074-14552093 Msa0239940:intron 35.0%
!! TCAATTGGTTTGACCTAAGA+AGG - chr2_2:14552113-14552132 None:intergenic 35.0%
TTTCCTAGAGATACAAACGA+AGG + chr2_2:14552265-14552284 Msa0239940:intron 35.0%
!!! AAATGGAGCATTGGTTTTCT+GGG + chr2_2:14552535-14552554 Msa0239940:intron 35.0%
TGGAATTAGAACATGTTGAG+GGG + chr2_2:14552707-14552726 Msa0239940:intron 35.0%
!! AAGGTTGGTAAAGAAATGAG+GGG - chr2_2:14552774-14552793 None:intergenic 35.0%
AATTGCAGATCGTAAAAGGT+TGG - chr2_2:14552789-14552808 None:intergenic 35.0%
! GTTCCTGATTCGAGTTTAAT+TGG + chr2_2:14552889-14552908 Msa0239940:CDS 35.0%
CTTCCAATTAAACTCGAATC+AGG - chr2_2:14552895-14552914 None:intergenic 35.0%
! TGAGTTGAGATTAGAGTTGT+TGG - chr2_2:14552995-14553014 None:intergenic 35.0%
! CAGGTAAGTATTTTGCTAGT+CGG - chr2_2:14553447-14553466 None:intergenic 35.0%
AAATCAGATTGACGTACCAT+CGG - chr2_2:14553479-14553498 None:intergenic 35.0%
AAGACTGACAAGAAAGAAAC+GGG + chr2_2:14553629-14553648 Msa0239940:CDS 35.0%
GATATGCTTTCCAATCTAGA+TGG + chr2_2:14553653-14553672 Msa0239940:CDS 35.0%
! ATAGCTTCCCTTTTTGCTTT+TGG + chr2_2:14553688-14553707 Msa0239940:intron 35.0%
!! TAGCTTCCCTTTTTGCTTTT+GGG + chr2_2:14553689-14553708 Msa0239940:intron 35.0%
! GAGATGGTTCTATTTAGTTC+AGG - chr2_2:14553782-14553801 None:intergenic 35.0%
GGATGTAAAACCAAATGAGA+TGG - chr2_2:14553798-14553817 None:intergenic 35.0%
!!! TCTCATTTGGTTTTACATCC+TGG + chr2_2:14553798-14553817 Msa0239940:intron 35.0%
ATAATGTTACATGTCTGCAG+GGG + chr2_2:14553975-14553994 Msa0239940:intron 35.0%
! CATTTGAAGCTTTAGTGCTT+CGG - chr2_2:14554012-14554031 None:intergenic 35.0%
ACAATTAAGGATAGAAGCTC+AGG + chr2_2:14554348-14554367 Msa0239940:CDS 35.0%
CAATTAAGGATAGAAGCTCA+GGG + chr2_2:14554349-14554368 Msa0239940:CDS 35.0%
! AGCGTATTTGAAATCAGACA+TGG + chr2_2:14554684-14554703 Msa0239940:CDS 35.0%
AAGAAGGATCAAAGTGATCT+CGG - chr2_2:14554777-14554796 None:intergenic 35.0%
GATCACTTTGATCCTTCTTT+AGG + chr2_2:14554778-14554797 Msa0239940:CDS 35.0%
! TGTGATTTTGTGTAATCTGC+AGG + chr2_2:14554851-14554870 Msa0239940:exon 35.0%
! GTGATTTTGTGTAATCTGCA+GGG + chr2_2:14554852-14554871 Msa0239940:exon 35.0%
!! TATGCATTCTTAGTTGATGG+AGG + chr2_2:14554899-14554918 Msa0239940:three_prime_UTR 35.0%
! GTCATATGTTCCCATTTTCA+AGG + chr2_2:14555045-14555064 Msa0239940:three_prime_UTR 35.0%
TACACAGCTCCGAAATTGAA+GGG + chr2_2:14550959-14550978 Msa0239940:five_prime_UTR 40.0%
CAGCACAAAACTACACAAGA+AGG - chr2_2:14551276-14551295 None:intergenic 40.0%
!!! TGTGTAGTTTTGTGCTGTGT+TGG + chr2_2:14551279-14551298 Msa0239940:intron 40.0%
! TTCAGCTTTTGTTGATGTGG+GGG + chr2_2:14551345-14551364 Msa0239940:intron 40.0%
!!! TTGCTGGGTTTTTTCTGTTG+TGG + chr2_2:14551373-14551392 Msa0239940:intron 40.0%
!!! TGCTGGGTTTTTTCTGTTGT+GGG + chr2_2:14551374-14551393 Msa0239940:intron 40.0%
TTCAGCCATGATGAGTGTTA+TGG + chr2_2:14551485-14551504 Msa0239940:intron 40.0%
TCAGCCATGATGAGTGTTAT+GGG + chr2_2:14551486-14551505 Msa0239940:intron 40.0%
!!! CAACTCCACTTTTTAGGGTT+TGG + chr2_2:14551721-14551740 Msa0239940:intron 40.0%
!!! ACTCCACTTTTTAGGGTTTG+GGG + chr2_2:14551723-14551742 Msa0239940:intron 40.0%
AAACCCCAAACCCTAAAAAG+TGG - chr2_2:14551729-14551748 None:intergenic 40.0%
!!! ACTTTTTAGGGTTTGGGGTT+TGG + chr2_2:14551728-14551747 Msa0239940:intron 40.0%
!!! CTTTTTAGGGTTTGGGGTTT+GGG + chr2_2:14551729-14551748 Msa0239940:intron 40.0%
!!! TTTTTAGGGTTTGGGGTTTG+GGG + chr2_2:14551730-14551749 Msa0239940:intron 40.0%
ACGAAAGGTTACCTATGCTA+GGG + chr2_2:14551762-14551781 Msa0239940:intron 40.0%
TAACATCCTTTCCCTAGCAT+AGG - chr2_2:14551776-14551795 None:intergenic 40.0%
TTTGAACCCAAGACCTTCTT+AGG + chr2_2:14552097-14552116 Msa0239940:intron 40.0%
GTTTGACCTAAGAAGGTCTT+GGG - chr2_2:14552106-14552125 None:intergenic 40.0%
AAACTTCTTGTATGGGTGGA+TGG - chr2_2:14552151-14552170 None:intergenic 40.0%
TGGTAAACTTCTTGTATGGG+TGG - chr2_2:14552155-14552174 None:intergenic 40.0%
AAAGTCAAGCCTCATGGAAT+AGG - chr2_2:14552246-14552265 None:intergenic 40.0%
TAGGAAAAAGTCAAGCCTCA+TGG - chr2_2:14552252-14552271 None:intergenic 40.0%
CATCCTTCGTTTGTATCTCT+AGG - chr2_2:14552271-14552290 None:intergenic 40.0%
!!! CAAATGGAGCATTGGTTTTC+TGG + chr2_2:14552534-14552553 Msa0239940:intron 40.0%
!!! AATGGAGCATTGGTTTTCTG+GGG + chr2_2:14552536-14552555 Msa0239940:intron 40.0%
CTCGAATCAGGAACATTAGT+TGG - chr2_2:14552883-14552902 None:intergenic 40.0%
GGAAGCAATCCATTAGTTCA+TGG + chr2_2:14552910-14552929 Msa0239940:CDS 40.0%
! ATGTGCTGACCATGAACTAA+TGG - chr2_2:14552922-14552941 None:intergenic 40.0%
ATGGGGAAAATACTCACGAT+CGG - chr2_2:14553088-14553107 None:intergenic 40.0%
! ATAACTCTGAGGACACCTTT+GGG - chr2_2:14553287-14553306 None:intergenic 40.0%
!! AAAGGTGTCCTCAGAGTTAT+GGG + chr2_2:14553287-14553306 Msa0239940:CDS 40.0%
! TGCTAGTCGGTATTTCTGTA+TGG - chr2_2:14553434-14553453 None:intergenic 40.0%
AGACTGACAAGAAAGAAACG+GGG + chr2_2:14553630-14553649 Msa0239940:CDS 40.0%
TCACGATGAACCATCTAGAT+TGG - chr2_2:14553666-14553685 None:intergenic 40.0%
ACTAGTGCAAATTTGACTCC+AGG - chr2_2:14553819-14553838 None:intergenic 40.0%
TAATGTTACATGTCTGCAGG+GGG + chr2_2:14553976-14553995 Msa0239940:intron 40.0%
! CGAAGCACTAAAGCTTCAAA+TGG + chr2_2:14554010-14554029 Msa0239940:CDS 40.0%
AGCACTAAAGCTTCAAATGG+AGG + chr2_2:14554013-14554032 Msa0239940:CDS 40.0%
GAAGCGACTACATGAACAAT+TGG + chr2_2:14554040-14554059 Msa0239940:CDS 40.0%
CAGATCAGATCAGTTCAACA+AGG - chr2_2:14554076-14554095 None:intergenic 40.0%
TGCAGAGACAGCTACAATTA+AGG + chr2_2:14554335-14554354 Msa0239940:CDS 40.0%
!! GCTCTTTAGCAGCTTTTTCT+GGG - chr2_2:14554519-14554538 None:intergenic 40.0%
!! TGCTCTTTAGCAGCTTTTTC+TGG - chr2_2:14554520-14554539 None:intergenic 40.0%
TAAAAAGCAACGAGTAAGCG+TGG + chr2_2:14554657-14554676 Msa0239940:CDS 40.0%
AGAAGGATCAAAGTGATCTC+GGG - chr2_2:14554776-14554795 None:intergenic 40.0%
CTTGTAGACATGCCTAAAGA+AGG - chr2_2:14554793-14554812 None:intergenic 40.0%
TTAGGCATGTCTACAAGAAG+TGG + chr2_2:14554796-14554815 Msa0239940:CDS 40.0%
TACAAGAAGTGGTGAGGAAT+TGG + chr2_2:14554807-14554826 Msa0239940:CDS 40.0%
CTACACAGCTCCGAAATTGA+AGG + chr2_2:14550958-14550977 Msa0239940:five_prime_UTR 45.0%
ACACAGCTCCGAAATTGAAG+GGG + chr2_2:14550960-14550979 Msa0239940:five_prime_UTR 45.0%
!!! GGTGACCCTTTTGGCTTATT+TGG + chr2_2:14551162-14551181 Msa0239940:intron 45.0%
!!! TTTTAGGGTTTGGGGTTTGG+GGG + chr2_2:14551731-14551750 Msa0239940:intron 45.0%
GACGAAAGGTTACCTATGCT+AGG + chr2_2:14551761-14551780 Msa0239940:intron 45.0%
AGGTTACCTATGCTAGGGAA+AGG + chr2_2:14551767-14551786 Msa0239940:intron 45.0%
! GCTAGCAGCATCAACGATTT+TGG - chr2_2:14551883-14551902 None:intergenic 45.0%
GGTTTGACCTAAGAAGGTCT+TGG - chr2_2:14552107-14552126 None:intergenic 45.0%
TCTTGTATGGGTGGATGGAT+GGG - chr2_2:14552146-14552165 None:intergenic 45.0%
TTCTTGTATGGGTGGATGGA+TGG - chr2_2:14552147-14552166 None:intergenic 45.0%
AACAGTGTGCCTATTCCATG+AGG + chr2_2:14552234-14552253 Msa0239940:intron 45.0%
! TTTAGACCCATGCTTTTGCC+CGG + chr2_2:14552422-14552441 Msa0239940:intron 45.0%
TGCTGTGTGCAGAAAGTCAT+TGG + chr2_2:14552488-14552507 Msa0239940:intron 45.0%
!!! ATGGAGCATTGGTTTTCTGG+GGG + chr2_2:14552537-14552556 Msa0239940:intron 45.0%
ACTCAAAGCAACGTTTGCGT+TGG + chr2_2:14553010-14553029 Msa0239940:CDS 45.0%
! TGAACGCTTTGTTGATGCTG+TGG + chr2_2:14553047-14553066 Msa0239940:CDS 45.0%
TGGGGAAAATACTCACGATC+GGG - chr2_2:14553087-14553106 None:intergenic 45.0%
! CATAACTCTGAGGACACCTT+TGG - chr2_2:14553288-14553307 None:intergenic 45.0%
!! CAAAGGTGTCCTCAGAGTTA+TGG + chr2_2:14553286-14553305 Msa0239940:CDS 45.0%
CTTGTACACCCATAACTCTG+AGG - chr2_2:14553298-14553317 None:intergenic 45.0%
CTCAGAGTTATGGGTGTACA+AGG + chr2_2:14553296-14553315 Msa0239940:CDS 45.0%
CCTGCAAATGGCTCTTAACA+TGG - chr2_2:14553334-14553353 None:intergenic 45.0%
CCATGTTAAGAGCCATTTGC+AGG + chr2_2:14553331-14553350 Msa0239940:CDS 45.0%
GAGTGCACATTACCTGCAAA+TGG - chr2_2:14553346-14553365 None:intergenic 45.0%
TTGCAGGTAATGTGCACTCT+AGG + chr2_2:14553347-14553366 Msa0239940:intron 45.0%
CACTCTAGGTCCTCTTAATG+TGG + chr2_2:14553361-14553380 Msa0239940:intron 45.0%
GACTGACAAGAAAGAAACGG+GGG + chr2_2:14553631-14553650 Msa0239940:CDS 45.0%
ATTCAATGCCTCAAACCCGT+TGG + chr2_2:14553873-14553892 Msa0239940:intron 45.0%
!! TTTTTCCACCAACGGGTTTG+AGG - chr2_2:14553884-14553903 None:intergenic 45.0%
GCGACTACATGAACAATTGG+AGG + chr2_2:14554043-14554062 Msa0239940:CDS 45.0%
CTTATTGTCGAGCTCCTGTT+GGG - chr2_2:14554476-14554495 None:intergenic 45.0%
TCTTATTGTCGAGCTCCTGT+TGG - chr2_2:14554477-14554496 None:intergenic 45.0%
GCTCGACAATAAGACTGAAC+CGG + chr2_2:14554483-14554502 Msa0239940:CDS 45.0%
!! TAGCAGCTTTTTCTGGGTCA+GGG - chr2_2:14554513-14554532 None:intergenic 45.0%
!! TTAGCAGCTTTTTCTGGGTC+AGG - chr2_2:14554514-14554533 None:intergenic 45.0%
TCTGCTGAAAGACTCTTTGC+TGG - chr2_2:14554547-14554566 None:intergenic 45.0%
!! AATCTGGTGTCATCGGTTCA+TGG - chr2_2:14554585-14554604 None:intergenic 45.0%
!! CAACCAGAATCTGGTGTCAT+CGG - chr2_2:14554592-14554611 None:intergenic 45.0%
TTAGTTGATCGCTCGCCATT+AGG - chr2_2:14554640-14554659 None:intergenic 45.0%
CTTGACTCCAGTATCTGATG+AGG - chr2_2:14554715-14554734 None:intergenic 45.0%
ATCTCGGGTAAGGAAAACAG+CGG - chr2_2:14554761-14554780 None:intergenic 45.0%
GATCAAAGTGATCTCGGGTA+AGG - chr2_2:14554771-14554790 None:intergenic 45.0%
CATGTCTACAAGAAGTGGTG+AGG + chr2_2:14554801-14554820 Msa0239940:CDS 45.0%
GGTGAGGAATTGGATAAGCT+TGG + chr2_2:14554817-14554836 Msa0239940:CDS 45.0%
GAGGAATTGGATAAGCTTGG+TGG + chr2_2:14554820-14554839 Msa0239940:CDS 45.0%
! AATTGGATAAGCTTGGTGGC+AGG + chr2_2:14554824-14554843 Msa0239940:CDS 45.0%
!! TTGAGTTTTTGGGCCCATCT+TGG + chr2_2:14555068-14555087 Msa0239940:three_prime_UTR 45.0%
!! TGAGTTTTTGGGCCCATCTT+GGG + chr2_2:14555069-14555088 Msa0239940:three_prime_UTR 45.0%
! AGCTTTTGTTGATGTGGGGG+AGG + chr2_2:14551348-14551367 Msa0239940:intron 50.0%
! TTTAGGGTTTGGGGTTTGGG+GGG + chr2_2:14551732-14551751 Msa0239940:intron 50.0%
TTGGGTTCAAACCCGGACAA+AGG - chr2_2:14552088-14552107 None:intergenic 50.0%
GAAGGTCTTGGGTTCAAACC+CGG - chr2_2:14552095-14552114 None:intergenic 50.0%
ATGGGTGGGGGTAGATCAAT+TGG - chr2_2:14552128-14552147 None:intergenic 50.0%
! TTAGACCCATGCTTTTGCCC+GGG + chr2_2:14552423-14552442 Msa0239940:intron 50.0%
CATGGTCAGCACATAGAGTC+TGG + chr2_2:14552928-14552947 Msa0239940:CDS 50.0%
GTCAGCACATAGAGTCTGGT+AGG + chr2_2:14552932-14552951 Msa0239940:CDS 50.0%
AGACTGTTTCCTCCACTACC+AGG - chr2_2:14552970-14552989 None:intergenic 50.0%
GTTGATGCTGTGGCTCAACT+TGG + chr2_2:14553057-14553076 Msa0239940:CDS 50.0%
GGGGAAAATACTCACGATCG+GGG - chr2_2:14553086-14553105 None:intergenic 50.0%
TTACCTGACTGCTCATCCGA+TGG + chr2_2:14553460-14553479 Msa0239940:CDS 50.0%
AAGAGCAACAGCGACTCAGT+GGG + chr2_2:14554395-14554414 Msa0239940:CDS 50.0%
TGGGACATATCTCCTGGAGT+TGG - chr2_2:14554457-14554476 None:intergenic 50.0%
TCCAGGAGATATGTCCCAAC+AGG + chr2_2:14554459-14554478 Msa0239940:CDS 50.0%
TCCTGTTGGGACATATCTCC+TGG - chr2_2:14554463-14554482 None:intergenic 50.0%
!! AGCAGCTTTTTCTGGGTCAG+GGG - chr2_2:14554512-14554531 None:intergenic 50.0%
GAACCGATGACACCAGATTC+TGG + chr2_2:14554586-14554605 Msa0239940:CDS 50.0%
! CCAGATTCTGGTTGCCAAGT+CGG + chr2_2:14554598-14554617 Msa0239940:CDS 50.0%
TCTCCTGCTGACAGTCCTAA+TGG + chr2_2:14554622-14554641 Msa0239940:CDS 50.0%
GGTGCTTCCTCATCAGATAC+TGG + chr2_2:14554705-14554724 Msa0239940:CDS 50.0%
! TGATGTGGGGGAGGTTTTGC+TGG + chr2_2:14551357-14551376 Msa0239940:intron 55.0%
! GATGTGGGGGAGGTTTTGCT+GGG + chr2_2:14551358-14551377 Msa0239940:intron 55.0%
! TTAGGGTTTGGGGTTTGGGG+GGG + chr2_2:14551733-14551752 Msa0239940:intron 55.0%
TATGGGTGGATGGATGGGTG+GGG - chr2_2:14552141-14552160 None:intergenic 55.0%
GTATGGGTGGATGGATGGGT+GGG - chr2_2:14552142-14552161 None:intergenic 55.0%
TGTATGGGTGGATGGATGGG+TGG - chr2_2:14552143-14552162 None:intergenic 55.0%
! GTGAACCCGGGCAAAAGCAT+GGG - chr2_2:14552431-14552450 None:intergenic 55.0%
AGTGAACCCGGGCAAAAGCA+TGG - chr2_2:14552432-14552451 None:intergenic 55.0%
GAGGGGTGACTCAAACATGC+AGG - chr2_2:14552757-14552776 None:intergenic 55.0%
!! AGAGTCTGGTAGGAGCGCTA+TGG + chr2_2:14552942-14552961 Msa0239940:CDS 55.0%
AGCGCTATGGATCCTGGTAG+TGG + chr2_2:14552955-14552974 Msa0239940:CDS 55.0%
!! GCTATGGATCCTGGTAGTGG+AGG + chr2_2:14552958-14552977 Msa0239940:CDS 55.0%
GATGCTGTGGCTCAACTTGG+TGG + chr2_2:14553060-14553079 Msa0239940:CDS 55.0%
GGGTGTGGCACCTAAGGAAA+AGG - chr2_2:14553267-14553286 None:intergenic 55.0%
! TCCTTAGGTGCCACACCCAA+AGG + chr2_2:14553269-14553288 Msa0239940:intron 55.0%
ACCTTTGGGTGTGGCACCTA+AGG - chr2_2:14553273-14553292 None:intergenic 55.0%
TCTGAGGACACCTTTGGGTG+TGG - chr2_2:14553282-14553301 None:intergenic 55.0%
GTACCATCGGATGAGCAGTC+AGG - chr2_2:14553466-14553485 None:intergenic 55.0%
CAATGCCTCAAACCCGTTGG+TGG + chr2_2:14553876-14553895 Msa0239940:intron 55.0%
GAAGAGCAACAGCGACTCAG+TGG + chr2_2:14554394-14554413 Msa0239940:CDS 55.0%
AGAGCAACAGCGACTCAGTG+GGG + chr2_2:14554396-14554415 Msa0239940:CDS 55.0%
!! GGGGTTCTTTCAGAAGCACC+CGG + chr2_2:14554415-14554434 Msa0239940:CDS 55.0%
!! TGGTGCTGATACACCAGAGC+CGG - chr2_2:14554437-14554456 None:intergenic 55.0%
GGTGTATCAGCACCAACTCC+AGG + chr2_2:14554442-14554461 Msa0239940:CDS 55.0%
! TTTTCTGGGTCAGGGGTTGC+CGG - chr2_2:14554505-14554524 None:intergenic 55.0%
!! CCGACTTGGCAACCAGAATC+TGG - chr2_2:14554601-14554620 None:intergenic 55.0%
TGTCAGCAGGAGAACCGACT+TGG - chr2_2:14554615-14554634 None:intergenic 55.0%
TCGCCATTAGGACTGTCAGC+AGG - chr2_2:14554628-14554647 None:intergenic 55.0%
! GCGGTTGGTTGCTGGTATGA+TGG - chr2_2:14554742-14554761 None:intergenic 55.0%
! GGAAAACAGCGGTTGGTTGC+TGG - chr2_2:14554750-14554769 None:intergenic 55.0%
CGGGTAAGGAAAACAGCGGT+TGG - chr2_2:14554757-14554776 None:intergenic 55.0%
! TAGGGTTTGGGGTTTGGGGG+GGG + chr2_2:14551734-14551753 Msa0239940:intron 60.0%
ATGGGTGGATGGATGGGTGG+GGG - chr2_2:14552140-14552159 None:intergenic 60.0%
!! GGTAGGAGCGCTATGGATCC+TGG + chr2_2:14552949-14552968 Msa0239940:CDS 60.0%
!! CTTTCAGAAGCACCCGGCTC+TGG + chr2_2:14554421-14554440 Msa0239940:CDS 60.0%
!! GGTGCTGATACACCAGAGCC+GGG - chr2_2:14554436-14554455 None:intergenic 60.0%
! AGGGTTTGGGGTTTGGGGGG+GGG + chr2_2:14551735-14551754 Msa0239940:intron 65.0%
! GGGTTTGGGGTTTGGGGGGG+GGG + chr2_2:14551736-14551755 Msa0239940:intron 70.0%
! GGTTTGGGGTTTGGGGGGGG+GGG + chr2_2:14551737-14551756 Msa0239940:intron 70.0%
! GTTTGGGGTTTGGGGGGGGG+GGG + chr2_2:14551738-14551757 Msa0239940:intron 70.0%
! TTTGGGGTTTGGGGGGGGGG+GGG + chr2_2:14551739-14551758 Msa0239940:intron 70.0%
TTGGGGGGGGGGGGGACGAA+AGG + chr2_2:14551747-14551766 Msa0239940:intron 75.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 14550888 14555097 14550888 ID=Msa0239940;Name=Msa0239940
chr2_2 mRNA 14550888 14555097 14550888 ID=Msa0239940-mRNA-1;Parent=Msa0239940;Name=Msa0239940-mRNA-1;_AED=0.06;_eAED=0.06;_QI=151|1|1|1|0.83|0.71|7|242|330
chr2_2 exon 14550888 14551027 14550888 ID=Msa0239940-mRNA-1:exon:6016;Parent=Msa0239940-mRNA-1
chr2_2 exon 14552860 14553090 14552860 ID=Msa0239940-mRNA-1:exon:6017;Parent=Msa0239940-mRNA-1
chr2_2 exon 14553276 14553352 14553276 ID=Msa0239940-mRNA-1:exon:6018;Parent=Msa0239940-mRNA-1
chr2_2 exon 14553439 14553481 14553439 ID=Msa0239940-mRNA-1:exon:6019;Parent=Msa0239940-mRNA-1
chr2_2 exon 14553621 14553681 14553621 ID=Msa0239940-mRNA-1:exon:6020;Parent=Msa0239940-mRNA-1
chr2_2 exon 14553995 14554064 14553995 ID=Msa0239940-mRNA-1:exon:6021;Parent=Msa0239940-mRNA-1
chr2_2 exon 14554334 14555097 14554334 ID=Msa0239940-mRNA-1:exon:6022;Parent=Msa0239940-mRNA-1
chr2_2 five_prime_UTR 14550888 14551027 14550888 ID=Msa0239940-mRNA-1:five_prime_utr;Parent=Msa0239940-mRNA-1
chr2_2 five_prime_UTR 14552860 14552870 14552860 ID=Msa0239940-mRNA-1:five_prime_utr;Parent=Msa0239940-mRNA-1
chr2_2 CDS 14552871 14553090 14552871 ID=Msa0239940-mRNA-1:cds;Parent=Msa0239940-mRNA-1
chr2_2 CDS 14553276 14553352 14553276 ID=Msa0239940-mRNA-1:cds;Parent=Msa0239940-mRNA-1
chr2_2 CDS 14553439 14553481 14553439 ID=Msa0239940-mRNA-1:cds;Parent=Msa0239940-mRNA-1
chr2_2 CDS 14553621 14553681 14553621 ID=Msa0239940-mRNA-1:cds;Parent=Msa0239940-mRNA-1
chr2_2 CDS 14553995 14554064 14553995 ID=Msa0239940-mRNA-1:cds;Parent=Msa0239940-mRNA-1
chr2_2 CDS 14554334 14554855 14554334 ID=Msa0239940-mRNA-1:cds;Parent=Msa0239940-mRNA-1
chr2_2 three_prime_UTR 14554856 14555097 14554856 ID=Msa0239940-mRNA-1:three_prime_utr;Parent=Msa0239940-mRNA-1
Gene Sequence

>Msa0239940

ATGTATCAGCCAACTAATGTTCCTGATTCGAGTTTAATTGGAAGCAATCCATTAGTTCATGGTCAGCACATAGAGTCTGGTAGGAGCGCTATGGATCCTGGTAGTGGAGGAAACAGTCTTGCCAACAACTCTAATCTCAACTCAAAGCAACGTTTGCGTTGGACACATGAGCTACATGAACGCTTTGTTGATGCTGTGGCTCAACTTGGTGGCCCCGATCGTGCCACACCCAAAGGTGTCCTCAGAGTTATGGGTGTACAAGGCTTAACAATTTACCATGTTAAGAGCCATTTGCAGAAATACCGACTAGCAAAATACTTACCTGACTGCTCATCCGATGAGGGGAAAAAGACTGACAAGAAAGAAACGGGGGATATGCTTTCCAATCTAGATGGTTCATCGGGGATGCAGATTACCGAAGCACTAAAGCTTCAAATGGAGGTACAGAAGCGACTACATGAACAATTGGAGGTGCAGAGACAGCTACAATTAAGGATAGAAGCTCAGGGAAAATATTTGAAAAAGATAATCGAAGAGCAACAGCGACTCAGTGGGGTTCTTTCAGAAGCACCCGGCTCTGGTGTATCAGCACCAACTCCAGGAGATATGTCCCAACAGGAGCTCGACAATAAGACTGAACCGGCAACCCCTGACCCAGAAAAAGCTGCTAAAGAGCATGCACCAGCAAAGAGTCTTTCAGCAGAATCTTTTTCATCTCACCATGAACCGATGACACCAGATTCTGGTTGCCAAGTCGGTTCTCCTGCTGACAGTCCTAATGGCGAGCGATCAACTAAAAAGCAACGAGTAAGCGTGGAAGAAGCGTATTTGAAATCAGACATGGTGCTTCCTCATCAGATACTGGAGTCAAGCATGCCATCATACCAGCAACCAACCGCTGTTTTCCTTACCCGAGATCACTTTGATCCTTCTTTAGGCATGTCTACAAGAAGTGGTGAGGAATTGGATAAGCTTGGTGGCAGGAATATGTGA
Protein sequence

>Msa0239940

MYQPTNVPDSSLIGSNPLVHGQHIESGRSAMDPGSGGNSLANNSNLNSKQRLRWTHELHERFVDAVAQLGGPDRATPKGVLRVMGVQGLTIYHVKSHLQKYRLAKYLPDCSSDEGKKTDKKETGDMLSNLDGSSGMQITEALKLQMEVQKRLHEQLEVQRQLQLRIEAQGKYLKKIIEEQQRLSGVLSEAPGSGVSAPTPGDMSQQELDNKTEPATPDPEKAAKEHAPAKSLSAESFSSHHEPMTPDSGCQVGSPADSPNGERSTKKQRVSVEEAYLKSDMVLPHQILESSMPSYQQPTAVFLTRDHFDPSLGMSTRSGEELDKLGGRNM*