AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0308220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0308220 MtrunA17_Chr2g0291021 97.273 330 9 0 1 330 1 330 0.0 663
Msa0308220 MtrunA17_Chr7g0254981 73.050 141 37 1 49 188 50 190 8.39e-69 216
Msa0308220 MtrunA17_Chr7g0261911 60.390 154 52 3 33 186 252 396 6.97e-53 181
Msa0308220 MtrunA17_Chr1g0175091 48.087 183 77 3 35 217 244 408 1.95e-51 176
Msa0308220 MtrunA17_Chr1g0190301 53.216 171 73 3 33 200 156 322 1.24e-48 167
Msa0308220 MtrunA17_Chr7g0258551 51.351 148 69 2 46 193 209 353 2.99e-47 164
Msa0308220 MtrunA17_Chr7g0274471 53.333 150 68 1 48 197 217 364 1.35e-46 163
Msa0308220 MtrunA17_Chr8g0356501 59.398 133 51 2 49 179 37 168 4.32e-44 154
Msa0308220 MtrunA17_Chr5g0409731 52.448 143 58 1 49 181 25 167 6.24e-44 152
Msa0308220 MtrunA17_Chr4g0042451 44.792 192 80 6 47 220 15 198 2.14e-43 152
Msa0308220 MtrunA17_Chr2g0310011 58.779 131 47 2 49 175 35 162 4.51e-42 149
Msa0308220 MtrunA17_Chr7g0242521 46.497 157 75 4 49 200 13 165 5.75e-41 143
Msa0308220 MtrunA17_Chr5g0417521 44.262 183 87 5 47 217 13 192 7.39e-41 146
Msa0308220 MtrunA17_Chr2g0321461 49.682 157 61 3 47 185 43 199 9.79e-41 146
Msa0308220 MtrunA17_Chr6g0461641 48.701 154 63 2 47 185 40 192 1.19e-40 146
Msa0308220 MtrunA17_Chr2g0290991 47.826 138 56 2 49 173 39 173 2.40e-34 127
Msa0308220 MtrunA17_Chr1g0197171 47.059 136 63 2 49 176 44 178 7.00e-33 123
Msa0308220 MtrunA17_Chr1g0175071 46.043 139 66 2 49 184 177 309 1.08e-30 118
Msa0308220 MtrunA17_Chr1g0175081 48.000 75 39 0 35 109 8 82 2.97e-22 89.7
Msa0308220 MtrunA17_Chr7g0258531 37.222 180 94 5 13 180 171 343 7.52e-22 94.7
Msa0308220 MtrunA17_Chr2g0288601 66.071 56 19 0 51 106 73 128 1.03e-16 75.5
Msa0308220 MtrunA17_Chr7g0243081 61.404 57 22 0 51 107 73 129 1.49e-16 79.3
Msa0308220 MtrunA17_Chr6g0479431 60.000 65 24 1 51 113 204 268 2.58e-16 79.0
Msa0308220 MtrunA17_Chr8g0366281 46.535 101 44 3 51 151 175 265 2.77e-16 78.6
Msa0308220 MtrunA17_Chr3g0095611 48.718 78 24 1 51 112 21 98 7.91e-16 75.1
Msa0308220 MtrunA17_Chr1g0198601 56.061 66 29 0 49 114 211 276 1.48e-15 76.6
Msa0308220 MtrunA17_Chr5g0410741 63.636 55 20 0 51 105 221 275 2.24e-15 76.3
Msa0308220 MtrunA17_Chr8g0379121 63.636 55 20 0 51 105 113 167 2.34e-15 76.6
Msa0308220 MtrunA17_Chr1g0162931 53.425 73 33 1 51 123 20 91 2.89e-15 75.1
Msa0308220 MtrunA17_Chr3g0109981 63.636 55 20 0 51 105 276 330 3.19e-15 76.3
Msa0308220 MtrunA17_Chr2g0320231 65.455 55 19 0 51 105 99 153 4.52e-15 75.5
Msa0308220 MtrunA17_Chr1g0154261 61.818 55 21 0 51 105 128 182 5.88e-15 74.3
Msa0308220 MtrunA17_Chr1g0210911 65.385 52 18 0 51 102 24 75 6.45e-15 74.7
Msa0308220 MtrunA17_Chr5g0402631 55.714 70 30 1 49 117 214 283 4.10e-14 72.4
Msa0308220 MtrunA17_Chr2g0283951 67.347 49 16 0 51 99 91 139 1.46e-13 70.9
Msa0308220 MtrunA17_Chr5g0422621 58.182 55 23 0 49 103 198 252 5.22e-13 68.9
Msa0308220 MtrunA17_Chr4g0062921 48.718 78 39 1 39 115 224 301 6.08e-13 69.3
Msa0308220 MtrunA17_Chr4g0009981 50.685 73 33 2 41 113 108 177 1.01e-12 67.4
Msa0308220 MtrunA17_Chr4g0045361 60.000 55 22 0 49 103 274 328 1.17e-12 68.2
Msa0308220 MtrunA17_Chr4g0009901 56.364 55 23 1 49 103 168 221 1.98e-12 66.6
Msa0308220 MtrunA17_Chr4g0031961 48.611 72 35 2 49 119 131 201 7.83e-12 65.1
Msa0308220 MtrunA17_Chr2g0324891 50.820 61 29 1 42 102 154 213 9.01e-12 65.5
Msa0308220 MtrunA17_Chr5g0400241 43.038 79 44 1 27 105 289 366 1.27e-11 65.5
Msa0308220 MtrunA17_Chr7g0255711 58.182 55 22 1 49 103 91 144 1.33e-11 64.3
Msa0308220 MtrunA17_Chr4g0009851 52.308 65 28 2 49 113 169 230 2.08e-11 63.9
Msa0308220 MtrunA17_Chr2g0304841 54.545 55 24 1 49 103 213 266 2.56e-11 64.7
Msa0308220 MtrunA17_Chr4g0067981 58.182 55 22 1 49 103 215 268 3.46e-11 64.3
Msa0308220 MtrunA17_Chr4g0040481 53.704 54 24 1 49 102 158 210 4.84e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0308220 AT2G01060.1 66.779 298 69 9 43 330 9 286 3.04e-119 345
Msa0308220 AT2G01060.2 62.646 257 66 9 84 330 1 237 1.80e-88 265
Msa0308220 AT5G29000.1 57.692 156 63 2 29 183 170 323 2.09e-51 174
Msa0308220 AT5G29000.3 57.692 156 63 2 29 183 170 323 2.09e-51 174
Msa0308220 AT5G29000.2 57.692 156 63 2 29 183 213 366 4.06e-51 175
Msa0308220 AT3G04450.4 52.273 176 76 3 44 212 193 367 7.56e-51 174
Msa0308220 AT3G04450.2 52.273 176 76 3 44 212 193 367 7.56e-51 174
Msa0308220 AT3G04450.1 52.273 176 76 3 44 212 233 407 1.34e-50 174
Msa0308220 AT4G28610.1 52.432 185 80 4 41 225 217 393 4.26e-50 172
Msa0308220 AT5G06800.1 57.534 146 57 3 37 182 182 322 6.49e-50 171
Msa0308220 AT4G13640.1 59.574 141 47 2 49 179 37 177 1.91e-49 167
Msa0308220 AT3G13040.3 50.265 189 86 4 44 227 236 421 1.98e-49 171
Msa0308220 AT3G13040.1 50.265 189 86 4 44 227 236 421 1.98e-49 171
Msa0308220 AT3G13040.2 50.265 189 86 4 44 227 236 421 1.98e-49 171
Msa0308220 AT3G24120.1 58.156 141 49 2 49 179 41 181 7.71e-49 166
Msa0308220 AT5G06800.2 55.705 149 60 3 37 182 182 327 7.97e-49 168
Msa0308220 AT1G79430.2 61.268 142 47 3 49 185 34 172 2.76e-48 166
Msa0308220 AT4G13640.2 58.333 144 47 3 49 179 37 180 3.08e-47 161
Msa0308220 AT3G24120.2 56.944 144 49 3 49 179 41 184 9.96e-47 160
Msa0308220 AT3G04030.3 48.333 180 72 4 23 185 19 194 8.16e-43 152
Msa0308220 AT1G69580.2 49.693 163 65 3 47 192 32 194 9.30e-43 151
Msa0308220 AT2G20400.2 53.165 158 71 2 49 206 231 385 1.04e-42 152
Msa0308220 AT1G69580.1 49.091 165 62 4 47 192 32 193 1.05e-42 150
Msa0308220 AT2G20400.1 53.797 158 69 2 49 206 231 384 1.33e-42 152
Msa0308220 AT5G18240.1 51.316 152 61 1 47 185 43 194 4.04e-42 150
Msa0308220 AT5G18240.4 51.316 152 61 1 47 185 43 194 4.04e-42 150
Msa0308220 AT3G04030.2 47.778 180 72 5 23 185 19 193 3.73e-40 145
Msa0308220 AT5G18240.5 50.000 152 61 2 47 185 43 192 7.33e-39 142
Msa0308220 AT5G45580.1 45.223 157 56 3 49 182 7 156 4.34e-37 134
Msa0308220 AT3G04030.1 45.556 180 71 5 23 185 19 188 6.82e-37 136
Msa0308220 AT5G45580.2 45.860 157 55 3 49 182 23 172 8.00e-37 134
Msa0308220 AT5G18240.2 48.026 152 60 2 47 185 43 188 3.20e-36 135
Msa0308220 AT5G18240.3 48.026 152 60 2 47 185 43 188 3.20e-36 135
Msa0308220 AT3G12730.1 47.826 138 64 4 49 179 23 159 3.09e-35 128
Msa0308220 AT1G79430.1 54.545 110 42 3 81 185 1 107 4.73e-27 108
Msa0308220 AT5G29000.4 60.674 89 33 1 29 117 213 299 3.09e-25 103
Msa0308220 AT3G04450.3 64.286 70 25 0 44 113 233 302 3.48e-25 103
Msa0308220 AT3G04030.5 49.153 118 47 2 81 185 1 118 1.34e-24 102
Msa0308220 AT5G06800.3 60.563 71 26 1 45 115 188 256 5.54e-24 99.8
Msa0308220 AT3G04030.4 44.915 118 46 3 81 185 1 112 4.54e-19 86.7
Msa0308220 AT5G18240.6 43.220 118 48 2 81 185 1 112 5.52e-19 86.7
Msa0308220 AT2G06020.1 59.649 57 23 0 49 105 86 142 7.10e-18 83.2
Msa0308220 AT2G42660.1 65.517 58 20 0 51 108 52 109 2.94e-17 80.5
Msa0308220 AT2G40260.1 57.333 75 20 1 51 125 84 146 3.08e-17 82.4
Msa0308220 AT4G04605.1 69.231 52 16 0 51 102 16 67 4.16e-17 79.3
Msa0308220 AT1G32240.1 43.396 106 51 2 51 156 215 311 1.79e-16 80.1
Msa0308220 AT1G32240.2 43.396 106 51 2 51 156 215 311 1.99e-16 79.7
Msa0308220 AT5G42630.2 57.576 66 28 0 51 116 107 172 4.46e-16 76.6
Msa0308220 AT5G42630.1 57.576 66 28 0 51 116 107 172 5.78e-16 77.4
Msa0308220 AT3G12730.2 39.623 106 56 4 81 179 1 105 1.29e-15 74.3
Msa0308220 AT5G16560.1 60.000 65 24 1 51 113 221 285 1.62e-15 77.4
Msa0308220 AT1G14600.1 69.231 52 16 0 51 102 25 76 2.03e-15 75.5
Msa0308220 AT4G17695.1 63.636 55 20 0 51 105 166 220 3.12e-15 75.9
Msa0308220 AT2G02060.2 69.231 52 16 0 51 102 31 82 3.28e-15 74.7
Msa0308220 AT2G02060.1 69.231 52 16 0 51 102 31 82 3.28e-15 74.7
Msa0308220 AT2G38300.1 63.636 55 20 0 51 105 56 110 3.76e-15 75.9
Msa0308220 AT4G37180.1 60.000 55 22 0 49 103 210 264 3.44e-14 73.2
Msa0308220 AT4G37180.2 60.000 55 22 0 49 103 217 271 4.20e-14 72.8
Msa0308220 AT4G04580.1 54.545 55 25 0 51 105 16 70 1.20e-13 68.6
Msa0308220 AT1G49560.1 54.545 66 30 0 49 114 192 257 1.52e-13 70.9
Msa0308220 AT3G25790.1 58.182 55 23 0 54 108 201 255 2.98e-13 70.1
Msa0308220 AT1G25550.1 55.385 65 29 0 44 108 204 268 5.27e-13 69.3
Msa0308220 AT3G10760.1 48.101 79 40 1 25 103 81 158 7.96e-13 68.9
Msa0308220 AT1G13300.1 44.086 93 41 2 16 108 159 240 1.40e-12 68.2
Msa0308220 AT1G68670.1 48.052 77 40 0 42 118 208 284 1.82e-12 67.8
Msa0308220 AT5G05090.1 60.377 53 20 1 51 103 83 134 4.25e-12 65.9
Msa0308220 AT4G37180.3 55.172 58 26 0 49 106 210 267 6.17e-12 65.5
Msa0308220 AT5G58080.1 52.174 69 28 3 49 117 194 257 7.07e-12 66.6
Msa0308220 AT5G59570.2 58.491 53 21 1 51 103 143 194 9.86e-12 65.1
Msa0308220 AT5G59570.1 58.491 53 21 1 51 103 143 194 9.86e-12 65.1
Msa0308220 AT3G46640.2 58.491 53 21 1 51 103 146 197 1.21e-11 65.1
Msa0308220 AT3G46640.1 58.491 53 21 1 51 103 146 197 1.21e-11 65.1
Msa0308220 AT3G46640.3 58.491 53 21 1 51 103 146 197 1.24e-11 65.1
Msa0308220 AT2G20570.1 51.471 68 31 2 35 102 140 205 1.35e-11 65.5
Msa0308220 AT2G03500.1 53.226 62 29 0 47 108 231 292 1.63e-11 65.5
Msa0308220 AT2G01760.3 45.333 75 38 2 49 123 135 206 1.66e-11 64.7
Msa0308220 AT5G44190.1 46.154 65 34 1 38 102 136 199 2.79e-11 64.3
Msa0308220 AT2G01760.1 45.333 75 38 2 49 123 200 271 2.96e-11 64.3
Msa0308220 AT5G44190.2 46.154 65 34 1 38 102 136 199 3.20e-11 63.9
Msa0308220 AT2G01760.2 45.333 75 38 2 49 123 228 299 4.22e-11 63.9
Msa0308220 AT2G40970.1 56.364 55 23 1 49 103 105 158 4.60e-11 62.8
Msa0308220 AT3G25790.2 58.696 46 19 0 54 99 201 246 9.79e-11 62.0

Find 99 sgRNAs with CRISPR-Local

Find 297 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCCTGATTCGAGTTTAAT+TGG 0.223090 2_4:+2371757 Msa0308220:CDS
CTCTAAAAGTAATTAAATTA+AGG 0.260834 2_4:-2373855 None:intergenic
GATCACTTTGATCCTTCTTT+AGG 0.297515 2_4:+2373646 Msa0308220:CDS
TTTAGAGAACTGATTTGAAT+TGG 0.300478 2_4:+2373871 Msa0308220:three_prime_UTR
TTAGTTGATCGCTCGCCATT+AGG 0.329748 2_4:-2373505 None:intergenic
CCATGTTAAGAGCCATTTGC+AGG 0.349218 2_4:+2372199 Msa0308220:CDS
TACAAGAAGTGGTGAGGAAT+TGG 0.349818 2_4:+2373675 Msa0308220:CDS
TGCAGAGACAGCTACAATTA+AGG 0.349960 2_4:+2373203 Msa0308220:CDS
TGGATTTCATAGATCTCATC+TGG 0.363640 2_4:+2369874 Msa0308220:five_prime_UTR
CTTCCAATTAAACTCGAATC+AGG 0.368180 2_4:-2371760 None:intergenic
CATAACTCTGAGGACACCTT+TGG 0.375653 2_4:-2372153 None:intergenic
CAAAGGTGTCCTCAGAGTTA+TGG 0.377183 2_4:+2372154 Msa0308220:CDS
ATGTGCTGACCATGAACTAA+TGG 0.385679 2_4:-2371787 None:intergenic
GAACCGATGACACCAGATTC+TGG 0.386395 2_4:+2373454 Msa0308220:CDS
AAGACTGACAAGAAAGAAAC+GGG 0.390608 2_4:+2372497 Msa0308220:CDS
TGAGTTGAGATTAGAGTTGT+TGG 0.390955 2_4:-2371860 None:intergenic
GGAAAACAGCGGTTGGTTGC+TGG 0.394253 2_4:-2373615 None:intergenic
AAAGGTGTCCTCAGAGTTAT+GGG 0.402741 2_4:+2372155 Msa0308220:CDS
ACAATTAAGGATAGAAGCTC+AGG 0.410929 2_4:+2373216 Msa0308220:CDS
GGAAGCAATCCATTAGTTCA+TGG 0.415844 2_4:+2371778 Msa0308220:CDS
TTAGAGAACTGATTTGAATT+GGG 0.421756 2_4:+2373872 Msa0308220:three_prime_UTR
CATTTGAAGCTTTAGTGCTT+CGG 0.424328 2_4:-2372877 None:intergenic
GATATGCTTTCCAATCTAGA+TGG 0.424452 2_4:+2372521 Msa0308220:CDS
TCCTGTTGGGACATATCTCC+TGG 0.428112 2_4:-2373328 None:intergenic
TTATATGCATTCTTAGTTGA+TGG 0.434188 2_4:+2373764 Msa0308220:three_prime_UTR
TCTGCTGAAAGACTCTTTGC+TGG 0.439579 2_4:-2373412 None:intergenic
GGTGCTTCCTCATCAGATAC+TGG 0.444138 2_4:+2373573 Msa0308220:CDS
CTTTCAGAAGCACCCGGCTC+TGG 0.447624 2_4:+2373289 Msa0308220:CDS
CCGACTTGGCAACCAGAATC+TGG 0.448662 2_4:-2373466 None:intergenic
CTCGAATCAGGAACATTAGT+TGG 0.450860 2_4:-2371748 None:intergenic
TCTCCTGCTGACAGTCCTAA+TGG 0.454643 2_4:+2373490 Msa0308220:CDS
TCACGATGAACCATCTAGAT+TGG 0.455940 2_4:-2372531 None:intergenic
GTTGATGCTGTGGCTCAACT+TGG 0.466308 2_4:+2371925 Msa0308220:CDS
AAGAAGGATCAAAGTGATCT+CGG 0.468691 2_4:-2373642 None:intergenic
GAAGCGACTACATGAACAAT+TGG 0.469698 2_4:+2372908 Msa0308220:CDS
CGAAGCACTAAAGCTTCAAA+TGG 0.471790 2_4:+2372878 Msa0308220:CDS
TCCAGGAGATATGTCCCAAC+AGG 0.476349 2_4:+2373327 Msa0308220:CDS
ATAACTCTGAGGACACCTTT+GGG 0.476739 2_4:-2372152 None:intergenic
ATTAAATTGGTAGCCCAAGA+TGG 0.479332 2_4:-2373950 None:intergenic
TCTTATTGTCGAGCTCCTGT+TGG 0.481895 2_4:-2373342 None:intergenic
CTTATTGTCGAGCTCCTGTT+GGG 0.485642 2_4:-2373341 None:intergenic
CTCAGAGTTATGGGTGTACA+AGG 0.485843 2_4:+2372164 Msa0308220:CDS
GGTAGGAGCGCTATGGATCC+TGG 0.493466 2_4:+2371817 Msa0308220:CDS
CTACACAGCTCCGAAATTGA+AGG 0.498072 2_4:+2369827 Msa0308220:five_prime_UTR
CATGGTCAGCACATAGAGTC+TGG 0.500025 2_4:+2371796 Msa0308220:CDS
GAGTGCACATTACCTGCAAA+TGG 0.500488 2_4:-2372211 None:intergenic
AAAGACTGACAAGAAAGAAA+CGG 0.503951 2_4:+2372496 Msa0308220:CDS
TCCTTAGGTGCCACACCCAA+AGG 0.508503 2_4:+2372137 Msa0308220:intron
GATGCTGTGGCTCAACTTGG+TGG 0.512420 2_4:+2371928 Msa0308220:CDS
GCTCGACAATAAGACTGAAC+CGG 0.517717 2_4:+2373351 Msa0308220:CDS
TGGTGCTGATACACCAGAGC+CGG 0.522258 2_4:-2373302 None:intergenic
AATCTGGTGTCATCGGTTCA+TGG 0.523061 2_4:-2373450 None:intergenic
GATCAAAGTGATCTCGGGTA+AGG 0.525714 2_4:-2373636 None:intergenic
CCTGCAAATGGCTCTTAACA+TGG 0.525821 2_4:-2372199 None:intergenic
CCAGATTCTGGTTGCCAAGT+CGG 0.528854 2_4:+2373466 Msa0308220:CDS
GTACCATCGGATGAGCAGTC+AGG 0.531157 2_4:-2372331 None:intergenic
ACTCAAAGCAACGTTTGCGT+TGG 0.531602 2_4:+2371878 Msa0308220:CDS
AGAAGGATCAAAGTGATCTC+GGG 0.531953 2_4:-2373641 None:intergenic
GGTGTATCAGCACCAACTCC+AGG 0.533448 2_4:+2373310 Msa0308220:CDS
AGCGCTATGGATCCTGGTAG+TGG 0.534994 2_4:+2371823 Msa0308220:CDS
CTTGTAGACATGCCTAAAGA+AGG 0.541592 2_4:-2373658 None:intergenic
TTAGGCATGTCTACAAGAAG+TGG 0.546196 2_4:+2373664 Msa0308220:CDS
CGGGTAAGGAAAACAGCGGT+TGG 0.546421 2_4:-2373622 None:intergenic
GGTGCTGATACACCAGAGCC+GGG 0.552630 2_4:-2373301 None:intergenic
TCTGAGGACACCTTTGGGTG+TGG 0.554653 2_4:-2372147 None:intergenic
CAACCAGAATCTGGTGTCAT+CGG 0.558599 2_4:-2373457 None:intergenic
AGAGTCTGGTAGGAGCGCTA+TGG 0.562642 2_4:+2371810 Msa0308220:CDS
GCGGTTGGTTGCTGGTATGA+TGG 0.564870 2_4:-2373607 None:intergenic
TGTCAGCAGGAGAACCGACT+TGG 0.567410 2_4:-2373480 None:intergenic
AATTGGATAAGCTTGGTGGC+AGG 0.568213 2_4:+2373692 Msa0308220:CDS
TGGGACATATCTCCTGGAGT+TGG 0.568536 2_4:-2373322 None:intergenic
TTAAATTGGTAGCCCAAGAT+GGG 0.568543 2_4:-2373949 None:intergenic
AGACTGTTTCCTCCACTACC+AGG 0.568775 2_4:-2371835 None:intergenic
AAGAGCAACAGCGACTCAGT+GGG 0.575024 2_4:+2373263 Msa0308220:CDS
TACACAGCTCCGAAATTGAA+GGG 0.581590 2_4:+2369828 Msa0308220:five_prime_UTR
TATGCATTCTTAGTTGATGG+AGG 0.586098 2_4:+2373767 Msa0308220:three_prime_UTR
TCGCCATTAGGACTGTCAGC+AGG 0.590479 2_4:-2373493 None:intergenic
GGTGAGGAATTGGATAAGCT+TGG 0.592014 2_4:+2373685 Msa0308220:CDS
TTACCTGACTGCTCATCCGA+TGG 0.595863 2_4:+2372328 Msa0308220:CDS
GCGACTACATGAACAATTGG+AGG 0.598747 2_4:+2372911 Msa0308220:CDS
TGAACGCTTTGTTGATGCTG+TGG 0.601507 2_4:+2371915 Msa0308220:CDS
GGGGTTCTTTCAGAAGCACC+CGG 0.619430 2_4:+2373283 Msa0308220:CDS
GGATTTCATAGATCTCATCT+GGG 0.628659 2_4:+2369875 Msa0308220:five_prime_UTR
GTCAGCACATAGAGTCTGGT+AGG 0.630464 2_4:+2371800 Msa0308220:CDS
AGCACTAAAGCTTCAAATGG+AGG 0.636135 2_4:+2372881 Msa0308220:CDS
GAAGAGCAACAGCGACTCAG+TGG 0.642615 2_4:+2373262 Msa0308220:CDS
CTTGACTCCAGTATCTGATG+AGG 0.648174 2_4:-2373580 None:intergenic
CATGTCTACAAGAAGTGGTG+AGG 0.650625 2_4:+2373669 Msa0308220:CDS
GAGGAATTGGATAAGCTTGG+TGG 0.651592 2_4:+2373688 Msa0308220:CDS
CAATTAAGGATAGAAGCTCA+GGG 0.654933 2_4:+2373217 Msa0308220:CDS
GGGGAAAATACTCACGATCG+GGG 0.658151 2_4:-2371951 None:intergenic
GCTATGGATCCTGGTAGTGG+AGG 0.662575 2_4:+2371826 Msa0308220:CDS
ACACAGCTCCGAAATTGAAG+GGG 0.667923 2_4:+2369829 Msa0308220:five_prime_UTR
GACTGACAAGAAAGAAACGG+GGG 0.678708 2_4:+2372499 Msa0308220:CDS
AGCGTATTTGAAATCAGACA+TGG 0.689566 2_4:+2373552 Msa0308220:CDS
AGAGCAACAGCGACTCAGTG+GGG 0.711050 2_4:+2373264 Msa0308220:CDS
CTTGTACACCCATAACTCTG+AGG 0.715783 2_4:-2372163 None:intergenic
ATCTCGGGTAAGGAAAACAG+CGG 0.741797 2_4:-2373626 None:intergenic
AGACTGACAAGAAAGAAACG+GGG 0.753105 2_4:+2372498 Msa0308220:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAAATAAATAAATGTTAAT+TGG + chr2_4:2370302-2370321 Msa0308220:intron 10.0%
!!! TTTTATTAATTTCAATCAAA+TGG + chr2_4:2371387-2371406 Msa0308220:intron 10.0%
!!! TTGTATTGTTTTTACATTTT+TGG + chr2_4:2370010-2370029 Msa0308220:intron 15.0%
!!! AAGTTTGATTTTTTAAGTTT+TGG + chr2_4:2370098-2370117 Msa0308220:intron 15.0%
!! ATACAATATTAACAACAATT+AGG - chr2_4:2370277-2370296 None:intergenic 15.0%
!! ATAAATAAATGTTAATTGGT+TGG + chr2_4:2370306-2370325 Msa0308220:intron 15.0%
!! ATTAAATTTATGATCTATTG+TGG + chr2_4:2371198-2371217 Msa0308220:intron 15.0%
!!! GATTTAAAAACTTTTAGATA+AGG - chr2_4:2372104-2372123 None:intergenic 15.0%
!! CATTAAAAAAACAAGAATAA+TGG - chr2_4:2372258-2372277 None:intergenic 15.0%
!!! TAAAAAGTTATGTTTCTTTT+AGG + chr2_4:2372717-2372736 Msa0308220:intron 15.0%
!!! TTTTATTTTGAATCAAGTAA+AGG + chr2_4:2373106-2373125 Msa0308220:intron 15.0%
!!! CTCTAAAAGTAATTAAATTA+AGG - chr2_4:2373858-2373877 None:intergenic 15.0%
!!! AAAGCATTCATTGATTTTTA+GGG + chr2_4:2370405-2370424 Msa0308220:intron 20.0%
!! ATCACTAAGATAACATATAT+AGG - chr2_4:2370533-2370552 None:intergenic 20.0%
!!! AGTAATTTGATGATATTTGA+AGG + chr2_4:2370693-2370712 Msa0308220:intron 20.0%
!!! GTAATTTGATGATATTTGAA+GGG + chr2_4:2370694-2370713 Msa0308220:intron 20.0%
!!! TAATTTGATGATATTTGAAG+GGG + chr2_4:2370695-2370714 Msa0308220:intron 20.0%
!!! CTTTTTTTAATGACAAATGT+TGG + chr2_4:2370904-2370923 Msa0308220:intron 20.0%
!!! ACAGTTTGAAAAGTTTTTTA+TGG + chr2_4:2371492-2371511 Msa0308220:intron 20.0%
!! AAATCAGTAAGATCTAAATA+TGG - chr2_4:2372380-2372399 None:intergenic 20.0%
!! AGAATGCTTAATACTTTAAA+CGG - chr2_4:2372415-2372434 None:intergenic 20.0%
!! AAAGTTATCAGCAAATTTAA+AGG - chr2_4:2372454-2372473 None:intergenic 20.0%
!!! ACTTTTTAATTGTCTTATAG+AGG + chr2_4:2372469-2372488 Msa0308220:intron 20.0%
!!! CTTTTTAATTGTCTTATAGA+GGG + chr2_4:2372470-2372489 Msa0308220:intron 20.0%
!!! TTTTTAATTGTCTTATAGAG+GGG + chr2_4:2372471-2372490 Msa0308220:intron 20.0%
!! TATCATAATTATACAAGGAA+TGG + chr2_4:2372581-2372600 Msa0308220:intron 20.0%
!! TAGAAAAAATAACAGTTACA+AGG - chr2_4:2373172-2373191 None:intergenic 20.0%
!!! TTATTTTTTCTATGTGAAAC+AGG + chr2_4:2373180-2373199 Msa0308220:intron 20.0%
!! GAAAATTTTGTATACGTAGT+TGG + chr2_4:2369854-2369873 Msa0308220:five_prime_UTR 25.0%
! TTATAGAAATGAAGAAGAGA+AGG - chr2_4:2369945-2369964 None:intergenic 25.0%
! GAGAAAGTAAGGAATAAAAA+GGG - chr2_4:2369968-2369987 None:intergenic 25.0%
! AGAGAAAGTAAGGAATAAAA+AGG - chr2_4:2369969-2369988 None:intergenic 25.0%
! TAAAACCAAATAAGCCAAAA+GGG - chr2_4:2370039-2370058 None:intergenic 25.0%
! GCTTTCAAAAAGTTAAAACA+AGG - chr2_4:2370390-2370409 None:intergenic 25.0%
!!! GAAAGCATTCATTGATTTTT+AGG + chr2_4:2370404-2370423 Msa0308220:intron 25.0%
! GATGTTAATGTGATGAAAAA+TGG + chr2_4:2370446-2370465 Msa0308220:intron 25.0%
! TGTTATCTTAGTGATTAATC+AGG + chr2_4:2370538-2370557 Msa0308220:intron 25.0%
!!! ATTTTCAACTCCACTTTTTA+GGG + chr2_4:2370585-2370604 Msa0308220:intron 25.0%
! TAGCAAAGAATCTATCTAAT+AGG - chr2_4:2370722-2370741 None:intergenic 25.0%
! AAATTGAATTCAACTTTCTG+TGG + chr2_4:2370801-2370820 Msa0308220:intron 25.0%
!! TAGTTTAAGTTTCTTAGACA+TGG + chr2_4:2371079-2371098 Msa0308220:intron 25.0%
!! GTGTTTGTTAAATACTGTAT+TGG + chr2_4:2371427-2371446 Msa0308220:intron 25.0%
! CGTTGAAAAAAATTGTACAA+CGG - chr2_4:2371472-2371491 None:intergenic 25.0%
! AACTGAATCTGATTAAGAAT+TGG + chr2_4:2371555-2371574 Msa0308220:intron 25.0%
! ATTTCAGCTAACGAAAAAAT+GGG - chr2_4:2371974-2371993 None:intergenic 25.0%
! TTGGTTACATCATACATATT+TGG + chr2_4:2372007-2372026 Msa0308220:intron 25.0%
!! CAGATATCAAATACTTTTGT+TGG + chr2_4:2372032-2372051 Msa0308220:intron 25.0%
! TAAATTCATCATCTAGCATT+AGG - chr2_4:2372066-2372085 None:intergenic 25.0%
! TGGGTTATCATAATTATACA+AGG + chr2_4:2372576-2372595 Msa0308220:intron 25.0%
!!! TATAGATTTTTTTCCACCAA+CGG - chr2_4:2372760-2372779 None:intergenic 25.0%
! TTATATGCATTCTTAGTTGA+TGG + chr2_4:2373764-2373783 Msa0308220:three_prime_UTR 25.0%
!!! TTTAGAGAACTGATTTGAAT+TGG + chr2_4:2373871-2373890 Msa0308220:three_prime_UTR 25.0%
!!! TTAGAGAACTGATTTGAATT+GGG + chr2_4:2373872-2373891 Msa0308220:three_prime_UTR 25.0%
! AAAAACTCAACCTTGAAAAT+GGG - chr2_4:2373926-2373945 None:intergenic 25.0%
!!! ATTTTCAAGGTTGAGTTTTT+GGG + chr2_4:2373926-2373945 Msa0308220:three_prime_UTR 25.0%
TGAAAGAAAGAGAGAAAGTA+AGG - chr2_4:2369979-2369998 None:intergenic 30.0%
CTAAAACCAAATAAGCCAAA+AGG - chr2_4:2370040-2370059 None:intergenic 30.0%
! TTTTCAGCTTTTGTTGATGT+GGG + chr2_4:2370212-2370231 Msa0308220:intron 30.0%
ATAGCTAACATCAGTGAATT+GGG - chr2_4:2370489-2370508 None:intergenic 30.0%
TATAGCTAACATCAGTGAAT+TGG - chr2_4:2370490-2370509 None:intergenic 30.0%
TTCACTGATGTTAGCTATAA+AGG + chr2_4:2370491-2370510 Msa0308220:intron 30.0%
!!! GATTTTCAACTCCACTTTTT+AGG + chr2_4:2370584-2370603 Msa0308220:intron 30.0%
TATCTAGAGATAGATGATGA+TGG - chr2_4:2370779-2370798 None:intergenic 30.0%
!!! TTGATTTTTTCTCCTTTGTC+CGG + chr2_4:2370942-2370961 Msa0308220:intron 30.0%
ACTTGGTAAACTTCTTGTAT+GGG - chr2_4:2371027-2371046 None:intergenic 30.0%
AACTTGGTAAACTTCTTGTA+TGG - chr2_4:2371028-2371047 None:intergenic 30.0%
TTCTAAGTCAAACTAGAACT+TGG - chr2_4:2371044-2371063 None:intergenic 30.0%
!! AGTTCTAGTTTGACTTAGAA+TGG + chr2_4:2371044-2371063 Msa0308220:intron 30.0%
AAACTATTATTAGTGAACCC+GGG - chr2_4:2371312-2371331 None:intergenic 30.0%
CAAACTATTATTAGTGAACC+CGG - chr2_4:2371313-2371332 None:intergenic 30.0%
ATTTCAATCAAATGGAGCAT+TGG + chr2_4:2371395-2371414 Msa0308220:intron 30.0%
AAATTGTACAACGGATCATT+TGG - chr2_4:2371463-2371482 None:intergenic 30.0%
AACTCTCTTTCTTTCAATCA+TGG - chr2_4:2371534-2371553 None:intergenic 30.0%
ATTGGAATTAGAACATGTTG+AGG + chr2_4:2371573-2371592 Msa0308220:intron 30.0%
TTGGAATTAGAACATGTTGA+GGG + chr2_4:2371574-2371593 Msa0308220:intron 30.0%
AGACAGAAACACACAAAAAT+TGG - chr2_4:2371601-2371620 None:intergenic 30.0%
!! AAAGGTTGGTAAAGAAATGA+GGG - chr2_4:2371643-2371662 None:intergenic 30.0%
!! AAAAGGTTGGTAAAGAAATG+AGG - chr2_4:2371644-2371663 None:intergenic 30.0%
ACTTAATTGCAGATCGTAAA+AGG - chr2_4:2371661-2371680 None:intergenic 30.0%
TTTCAGCTAACGAAAAAATG+GGG - chr2_4:2371973-2371992 None:intergenic 30.0%
CATTTCAGCTAACGAAAAAA+TGG - chr2_4:2371975-2371994 None:intergenic 30.0%
TGAAATGTAATGCGTAGAAT+TGG + chr2_4:2371988-2372007 Msa0308220:intron 30.0%
TGCTAGATGATGAATTTATC+AGG + chr2_4:2372068-2372087 Msa0308220:intron 30.0%
! TCTATACTATCCTTTTCCTT+AGG + chr2_4:2372122-2372141 Msa0308220:intron 30.0%
!! ATAATGGATACCACATTAAG+AGG - chr2_4:2372242-2372261 None:intergenic 30.0%
ATTTAAAGGAGGAAACAAAG+TGG - chr2_4:2372440-2372459 None:intergenic 30.0%
GTTATCAGCAAATTTAAAGG+AGG - chr2_4:2372451-2372470 None:intergenic 30.0%
AAAGACTGACAAGAAAGAAA+CGG + chr2_4:2372496-2372515 Msa0308220:CDS 30.0%
TGATAACCCAAAAGCAAAAA+GGG - chr2_4:2372566-2372585 None:intergenic 30.0%
ATGATAACCCAAAAGCAAAA+AGG - chr2_4:2372567-2372586 None:intergenic 30.0%
CTAAATAGAACCATCTCATT+TGG + chr2_4:2372653-2372672 Msa0308220:intron 30.0%
!!! ATAGATTTTTTTCCACCAAC+GGG - chr2_4:2372759-2372778 None:intergenic 30.0%
TAATAATGTTACATGTCTGC+AGG + chr2_4:2372841-2372860 Msa0308220:intron 30.0%
AATAATGTTACATGTCTGCA+GGG + chr2_4:2372842-2372861 Msa0308220:intron 30.0%
GTGAGATGAAATGATGATAT+TGG + chr2_4:2373021-2373040 Msa0308220:intron 30.0%
AAGGAATAATAGACAGAATG+AGG + chr2_4:2373125-2373144 Msa0308220:intron 30.0%
AGGAATAATAGACAGAATGA+GGG + chr2_4:2373126-2373145 Msa0308220:intron 30.0%
CAAAAACTCAACCTTGAAAA+TGG - chr2_4:2373927-2373946 None:intergenic 30.0%
!! CATTTTCAAGGTTGAGTTTT+TGG + chr2_4:2373925-2373944 Msa0308220:three_prime_UTR 30.0%
! ATTTTCGTCCCCTTCAATTT+CGG - chr2_4:2369840-2369859 None:intergenic 35.0%
TGGATTTCATAGATCTCATC+TGG + chr2_4:2369874-2369893 Msa0308220:five_prime_UTR 35.0%
GGATTTCATAGATCTCATCT+GGG + chr2_4:2369875-2369894 Msa0308220:five_prime_UTR 35.0%
!!! TACATTTTTGGTGACCCTTT+TGG + chr2_4:2370022-2370041 Msa0308220:intron 35.0%
AGCACAAAACTACACAAGAA+GGG - chr2_4:2370144-2370163 None:intergenic 35.0%
! CTTTTCAGCTTTTGTTGATG+TGG + chr2_4:2370211-2370230 Msa0308220:intron 35.0%
! TTTCAGCTTTTGTTGATGTG+GGG + chr2_4:2370213-2370232 Msa0308220:intron 35.0%
AAAACCCATAACACTCATCA+TGG - chr2_4:2370362-2370381 None:intergenic 35.0%
ACTGATGTTAGCTATAAAGG+TGG + chr2_4:2370494-2370513 Msa0308220:intron 35.0%
!!! AACTCCACTTTTTAGGGTTT+GGG + chr2_4:2370591-2370610 Msa0308220:intron 35.0%
!!! TGATTTTTTCTCCTTTGTCC+GGG + chr2_4:2370943-2370962 Msa0308220:intron 35.0%
!! TCAATTGGTTTGACCTAAGA+AGG - chr2_4:2370982-2371001 None:intergenic 35.0%
TTTCCTAGAGATACAAACGA+AGG + chr2_4:2371134-2371153 Msa0308220:intron 35.0%
!!! AAATGGAGCATTGGTTTTCT+GGG + chr2_4:2371404-2371423 Msa0308220:intron 35.0%
TGGAATTAGAACATGTTGAG+GGG + chr2_4:2371575-2371594 Msa0308220:intron 35.0%
!! AAGGTTGGTAAAGAAATGAG+GGG - chr2_4:2371642-2371661 None:intergenic 35.0%
AATTGCAGATCGTAAAAGGT+TGG - chr2_4:2371657-2371676 None:intergenic 35.0%
! GTTCCTGATTCGAGTTTAAT+TGG + chr2_4:2371757-2371776 Msa0308220:CDS 35.0%
CTTCCAATTAAACTCGAATC+AGG - chr2_4:2371763-2371782 None:intergenic 35.0%
! TGAGTTGAGATTAGAGTTGT+TGG - chr2_4:2371863-2371882 None:intergenic 35.0%
! CAGGTAAGTATTTTGCTAGT+CGG - chr2_4:2372315-2372334 None:intergenic 35.0%
AAATCAGATTGACGTACCAT+CGG - chr2_4:2372347-2372366 None:intergenic 35.0%
AAGACTGACAAGAAAGAAAC+GGG + chr2_4:2372497-2372516 Msa0308220:CDS 35.0%
GATATGCTTTCCAATCTAGA+TGG + chr2_4:2372521-2372540 Msa0308220:CDS 35.0%
! ATAGCTTCCCTTTTTGCTTT+TGG + chr2_4:2372556-2372575 Msa0308220:intron 35.0%
!! TAGCTTCCCTTTTTGCTTTT+GGG + chr2_4:2372557-2372576 Msa0308220:intron 35.0%
! GAGATGGTTCTATTTAGTTC+AGG - chr2_4:2372650-2372669 None:intergenic 35.0%
GGATGTAAAACCAAATGAGA+TGG - chr2_4:2372666-2372685 None:intergenic 35.0%
!!! TCTCATTTGGTTTTACATCC+TGG + chr2_4:2372666-2372685 Msa0308220:intron 35.0%
ATAATGTTACATGTCTGCAG+GGG + chr2_4:2372843-2372862 Msa0308220:intron 35.0%
! CATTTGAAGCTTTAGTGCTT+CGG - chr2_4:2372880-2372899 None:intergenic 35.0%
ACAATTAAGGATAGAAGCTC+AGG + chr2_4:2373216-2373235 Msa0308220:CDS 35.0%
CAATTAAGGATAGAAGCTCA+GGG + chr2_4:2373217-2373236 Msa0308220:CDS 35.0%
! AGCGTATTTGAAATCAGACA+TGG + chr2_4:2373552-2373571 Msa0308220:CDS 35.0%
AAGAAGGATCAAAGTGATCT+CGG - chr2_4:2373645-2373664 None:intergenic 35.0%
GATCACTTTGATCCTTCTTT+AGG + chr2_4:2373646-2373665 Msa0308220:CDS 35.0%
! TGTGATTTTGTGTAATCTGC+AGG + chr2_4:2373719-2373738 Msa0308220:exon 35.0%
! GTGATTTTGTGTAATCTGCA+GGG + chr2_4:2373720-2373739 Msa0308220:exon 35.0%
!! TATGCATTCTTAGTTGATGG+AGG + chr2_4:2373767-2373786 Msa0308220:three_prime_UTR 35.0%
! GTCATATGTTCCCATTTTCA+AGG + chr2_4:2373913-2373932 Msa0308220:three_prime_UTR 35.0%
TACACAGCTCCGAAATTGAA+GGG + chr2_4:2369828-2369847 Msa0308220:five_prime_UTR 40.0%
CAGCACAAAACTACACAAGA+AGG - chr2_4:2370145-2370164 None:intergenic 40.0%
!!! TGTGTAGTTTTGTGCTGTGT+TGG + chr2_4:2370148-2370167 Msa0308220:intron 40.0%
! TTCAGCTTTTGTTGATGTGG+GGG + chr2_4:2370214-2370233 Msa0308220:intron 40.0%
!!! TTGCTGGGTTTTTTCTGTTG+TGG + chr2_4:2370242-2370261 Msa0308220:intron 40.0%
!!! TGCTGGGTTTTTTCTGTTGT+GGG + chr2_4:2370243-2370262 Msa0308220:intron 40.0%
TTCAGCCATGATGAGTGTTA+TGG + chr2_4:2370354-2370373 Msa0308220:intron 40.0%
TCAGCCATGATGAGTGTTAT+GGG + chr2_4:2370355-2370374 Msa0308220:intron 40.0%
!!! CAACTCCACTTTTTAGGGTT+TGG + chr2_4:2370590-2370609 Msa0308220:intron 40.0%
!!! ACTCCACTTTTTAGGGTTTG+GGG + chr2_4:2370592-2370611 Msa0308220:intron 40.0%
AAACCCCAAACCCTAAAAAG+TGG - chr2_4:2370598-2370617 None:intergenic 40.0%
!!! ACTTTTTAGGGTTTGGGGTT+TGG + chr2_4:2370597-2370616 Msa0308220:intron 40.0%
!!! CTTTTTAGGGTTTGGGGTTT+GGG + chr2_4:2370598-2370617 Msa0308220:intron 40.0%
!!! TTTTTAGGGTTTGGGGTTTG+GGG + chr2_4:2370599-2370618 Msa0308220:intron 40.0%
ACGAAAGGTTACCTATGCTA+GGG + chr2_4:2370631-2370650 Msa0308220:intron 40.0%
TAACATCCTTTCCCTAGCAT+AGG - chr2_4:2370645-2370664 None:intergenic 40.0%
TTTGAACCCAAGACCTTCTT+AGG + chr2_4:2370966-2370985 Msa0308220:intron 40.0%
GTTTGACCTAAGAAGGTCTT+GGG - chr2_4:2370975-2370994 None:intergenic 40.0%
AAACTTCTTGTATGGGTGGA+TGG - chr2_4:2371020-2371039 None:intergenic 40.0%
TGGTAAACTTCTTGTATGGG+TGG - chr2_4:2371024-2371043 None:intergenic 40.0%
AAAGTCAAGCCTCATGGAAT+AGG - chr2_4:2371115-2371134 None:intergenic 40.0%
TAGGAAAAAGTCAAGCCTCA+TGG - chr2_4:2371121-2371140 None:intergenic 40.0%
CATCCTTCGTTTGTATCTCT+AGG - chr2_4:2371140-2371159 None:intergenic 40.0%
!!! CAAATGGAGCATTGGTTTTC+TGG + chr2_4:2371403-2371422 Msa0308220:intron 40.0%
!!! AATGGAGCATTGGTTTTCTG+GGG + chr2_4:2371405-2371424 Msa0308220:intron 40.0%
CTCGAATCAGGAACATTAGT+TGG - chr2_4:2371751-2371770 None:intergenic 40.0%
GGAAGCAATCCATTAGTTCA+TGG + chr2_4:2371778-2371797 Msa0308220:CDS 40.0%
! ATGTGCTGACCATGAACTAA+TGG - chr2_4:2371790-2371809 None:intergenic 40.0%
ATGGGGAAAATACTCACGAT+CGG - chr2_4:2371956-2371975 None:intergenic 40.0%
! ATAACTCTGAGGACACCTTT+GGG - chr2_4:2372155-2372174 None:intergenic 40.0%
!! AAAGGTGTCCTCAGAGTTAT+GGG + chr2_4:2372155-2372174 Msa0308220:CDS 40.0%
! TGCTAGTCGGTATTTCTGTA+TGG - chr2_4:2372302-2372321 None:intergenic 40.0%
AGACTGACAAGAAAGAAACG+GGG + chr2_4:2372498-2372517 Msa0308220:CDS 40.0%
TCACGATGAACCATCTAGAT+TGG - chr2_4:2372534-2372553 None:intergenic 40.0%
ACTAGTGCAAATTTGACTCC+AGG - chr2_4:2372687-2372706 None:intergenic 40.0%
TAATGTTACATGTCTGCAGG+GGG + chr2_4:2372844-2372863 Msa0308220:intron 40.0%
! CGAAGCACTAAAGCTTCAAA+TGG + chr2_4:2372878-2372897 Msa0308220:CDS 40.0%
AGCACTAAAGCTTCAAATGG+AGG + chr2_4:2372881-2372900 Msa0308220:CDS 40.0%
GAAGCGACTACATGAACAAT+TGG + chr2_4:2372908-2372927 Msa0308220:CDS 40.0%
CAGATCAGATCAGTTCAACA+AGG - chr2_4:2372944-2372963 None:intergenic 40.0%
TGCAGAGACAGCTACAATTA+AGG + chr2_4:2373203-2373222 Msa0308220:CDS 40.0%
!! GCTCTTTAGCAGCTTTTTCT+GGG - chr2_4:2373387-2373406 None:intergenic 40.0%
!! TGCTCTTTAGCAGCTTTTTC+TGG - chr2_4:2373388-2373407 None:intergenic 40.0%
TAAAAAGCAACGAGTAAGCG+TGG + chr2_4:2373525-2373544 Msa0308220:CDS 40.0%
AGAAGGATCAAAGTGATCTC+GGG - chr2_4:2373644-2373663 None:intergenic 40.0%
CTTGTAGACATGCCTAAAGA+AGG - chr2_4:2373661-2373680 None:intergenic 40.0%
TTAGGCATGTCTACAAGAAG+TGG + chr2_4:2373664-2373683 Msa0308220:CDS 40.0%
TACAAGAAGTGGTGAGGAAT+TGG + chr2_4:2373675-2373694 Msa0308220:CDS 40.0%
CTACACAGCTCCGAAATTGA+AGG + chr2_4:2369827-2369846 Msa0308220:five_prime_UTR 45.0%
ACACAGCTCCGAAATTGAAG+GGG + chr2_4:2369829-2369848 Msa0308220:five_prime_UTR 45.0%
!!! GGTGACCCTTTTGGCTTATT+TGG + chr2_4:2370031-2370050 Msa0308220:intron 45.0%
!!! TTTTAGGGTTTGGGGTTTGG+GGG + chr2_4:2370600-2370619 Msa0308220:intron 45.0%
GACGAAAGGTTACCTATGCT+AGG + chr2_4:2370630-2370649 Msa0308220:intron 45.0%
AGGTTACCTATGCTAGGGAA+AGG + chr2_4:2370636-2370655 Msa0308220:intron 45.0%
! GCTAGCAGCATCAACGATTT+TGG - chr2_4:2370752-2370771 None:intergenic 45.0%
GGTTTGACCTAAGAAGGTCT+TGG - chr2_4:2370976-2370995 None:intergenic 45.0%
TCTTGTATGGGTGGATGGAT+GGG - chr2_4:2371015-2371034 None:intergenic 45.0%
TTCTTGTATGGGTGGATGGA+TGG - chr2_4:2371016-2371035 None:intergenic 45.0%
AACAGTGTGCCTATTCCATG+AGG + chr2_4:2371103-2371122 Msa0308220:intron 45.0%
! TTTAGACCCATGCTTTTGCC+CGG + chr2_4:2371291-2371310 Msa0308220:intron 45.0%
TGCTGTGTGCAGAAAGTCAT+TGG + chr2_4:2371357-2371376 Msa0308220:intron 45.0%
ACTCAAAGCAACGTTTGCGT+TGG + chr2_4:2371878-2371897 Msa0308220:CDS 45.0%
! TGAACGCTTTGTTGATGCTG+TGG + chr2_4:2371915-2371934 Msa0308220:CDS 45.0%
TGGGGAAAATACTCACGATC+GGG - chr2_4:2371955-2371974 None:intergenic 45.0%
! CATAACTCTGAGGACACCTT+TGG - chr2_4:2372156-2372175 None:intergenic 45.0%
!! CAAAGGTGTCCTCAGAGTTA+TGG + chr2_4:2372154-2372173 Msa0308220:CDS 45.0%
CTTGTACACCCATAACTCTG+AGG - chr2_4:2372166-2372185 None:intergenic 45.0%
CTCAGAGTTATGGGTGTACA+AGG + chr2_4:2372164-2372183 Msa0308220:CDS 45.0%
CCTGCAAATGGCTCTTAACA+TGG - chr2_4:2372202-2372221 None:intergenic 45.0%
CCATGTTAAGAGCCATTTGC+AGG + chr2_4:2372199-2372218 Msa0308220:CDS 45.0%
GAGTGCACATTACCTGCAAA+TGG - chr2_4:2372214-2372233 None:intergenic 45.0%
TTGCAGGTAATGTGCACTCT+AGG + chr2_4:2372215-2372234 Msa0308220:intron 45.0%
CACTCTAGGTCCTCTTAATG+TGG + chr2_4:2372229-2372248 Msa0308220:intron 45.0%
GACTGACAAGAAAGAAACGG+GGG + chr2_4:2372499-2372518 Msa0308220:CDS 45.0%
ATTCAATGCCTCAAACCCGT+TGG + chr2_4:2372741-2372760 Msa0308220:intron 45.0%
!! TTTTTCCACCAACGGGTTTG+AGG - chr2_4:2372752-2372771 None:intergenic 45.0%
GCGACTACATGAACAATTGG+AGG + chr2_4:2372911-2372930 Msa0308220:CDS 45.0%
CTTATTGTCGAGCTCCTGTT+GGG - chr2_4:2373344-2373363 None:intergenic 45.0%
TCTTATTGTCGAGCTCCTGT+TGG - chr2_4:2373345-2373364 None:intergenic 45.0%
GCTCGACAATAAGACTGAAC+CGG + chr2_4:2373351-2373370 Msa0308220:CDS 45.0%
!! TAGCAGCTTTTTCTGGGTCA+GGG - chr2_4:2373381-2373400 None:intergenic 45.0%
!! TTAGCAGCTTTTTCTGGGTC+AGG - chr2_4:2373382-2373401 None:intergenic 45.0%
TCTGCTGAAAGACTCTTTGC+TGG - chr2_4:2373415-2373434 None:intergenic 45.0%
!! AATCTGGTGTCATCGGTTCA+TGG - chr2_4:2373453-2373472 None:intergenic 45.0%
!! CAACCAGAATCTGGTGTCAT+CGG - chr2_4:2373460-2373479 None:intergenic 45.0%
TTAGTTGATCGCTCGCCATT+AGG - chr2_4:2373508-2373527 None:intergenic 45.0%
CTTGACTCCAGTATCTGATG+AGG - chr2_4:2373583-2373602 None:intergenic 45.0%
ATCTCGGGTAAGGAAAACAG+CGG - chr2_4:2373629-2373648 None:intergenic 45.0%
GATCAAAGTGATCTCGGGTA+AGG - chr2_4:2373639-2373658 None:intergenic 45.0%
CATGTCTACAAGAAGTGGTG+AGG + chr2_4:2373669-2373688 Msa0308220:CDS 45.0%
GGTGAGGAATTGGATAAGCT+TGG + chr2_4:2373685-2373704 Msa0308220:CDS 45.0%
GAGGAATTGGATAAGCTTGG+TGG + chr2_4:2373688-2373707 Msa0308220:CDS 45.0%
! AATTGGATAAGCTTGGTGGC+AGG + chr2_4:2373692-2373711 Msa0308220:CDS 45.0%
!! TTGAGTTTTTGGGCCCATCT+TGG + chr2_4:2373936-2373955 Msa0308220:three_prime_UTR 45.0%
!! TGAGTTTTTGGGCCCATCTT+GGG + chr2_4:2373937-2373956 Msa0308220:three_prime_UTR 45.0%
! AGCTTTTGTTGATGTGGGGG+AGG + chr2_4:2370217-2370236 Msa0308220:intron 50.0%
! TTTAGGGTTTGGGGTTTGGG+GGG + chr2_4:2370601-2370620 Msa0308220:intron 50.0%
TTGGGTTCAAACCCGGACAA+AGG - chr2_4:2370957-2370976 None:intergenic 50.0%
GAAGGTCTTGGGTTCAAACC+CGG - chr2_4:2370964-2370983 None:intergenic 50.0%
ATGGGTGGGGGTAGATCAAT+TGG - chr2_4:2370997-2371016 None:intergenic 50.0%
! TTAGACCCATGCTTTTGCCC+GGG + chr2_4:2371292-2371311 Msa0308220:intron 50.0%
CATGGTCAGCACATAGAGTC+TGG + chr2_4:2371796-2371815 Msa0308220:CDS 50.0%
GTCAGCACATAGAGTCTGGT+AGG + chr2_4:2371800-2371819 Msa0308220:CDS 50.0%
AGACTGTTTCCTCCACTACC+AGG - chr2_4:2371838-2371857 None:intergenic 50.0%
GTTGATGCTGTGGCTCAACT+TGG + chr2_4:2371925-2371944 Msa0308220:CDS 50.0%
GGGGAAAATACTCACGATCG+GGG - chr2_4:2371954-2371973 None:intergenic 50.0%
TTACCTGACTGCTCATCCGA+TGG + chr2_4:2372328-2372347 Msa0308220:CDS 50.0%
AAGAGCAACAGCGACTCAGT+GGG + chr2_4:2373263-2373282 Msa0308220:CDS 50.0%
TGGGACATATCTCCTGGAGT+TGG - chr2_4:2373325-2373344 None:intergenic 50.0%
TCCAGGAGATATGTCCCAAC+AGG + chr2_4:2373327-2373346 Msa0308220:CDS 50.0%
TCCTGTTGGGACATATCTCC+TGG - chr2_4:2373331-2373350 None:intergenic 50.0%
!! AGCAGCTTTTTCTGGGTCAG+GGG - chr2_4:2373380-2373399 None:intergenic 50.0%
GAACCGATGACACCAGATTC+TGG + chr2_4:2373454-2373473 Msa0308220:CDS 50.0%
! CCAGATTCTGGTTGCCAAGT+CGG + chr2_4:2373466-2373485 Msa0308220:CDS 50.0%
TCTCCTGCTGACAGTCCTAA+TGG + chr2_4:2373490-2373509 Msa0308220:CDS 50.0%
GGTGCTTCCTCATCAGATAC+TGG + chr2_4:2373573-2373592 Msa0308220:CDS 50.0%
! TGATGTGGGGGAGGTTTTGC+TGG + chr2_4:2370226-2370245 Msa0308220:intron 55.0%
! GATGTGGGGGAGGTTTTGCT+GGG + chr2_4:2370227-2370246 Msa0308220:intron 55.0%
! TTAGGGTTTGGGGTTTGGGG+GGG + chr2_4:2370602-2370621 Msa0308220:intron 55.0%
TATGGGTGGATGGATGGGTG+GGG - chr2_4:2371010-2371029 None:intergenic 55.0%
GTATGGGTGGATGGATGGGT+GGG - chr2_4:2371011-2371030 None:intergenic 55.0%
TGTATGGGTGGATGGATGGG+TGG - chr2_4:2371012-2371031 None:intergenic 55.0%
! GTGAACCCGGGCAAAAGCAT+GGG - chr2_4:2371300-2371319 None:intergenic 55.0%
AGTGAACCCGGGCAAAAGCA+TGG - chr2_4:2371301-2371320 None:intergenic 55.0%
GAGGGGTGACTCAAACATGC+AGG - chr2_4:2371625-2371644 None:intergenic 55.0%
!! AGAGTCTGGTAGGAGCGCTA+TGG + chr2_4:2371810-2371829 Msa0308220:CDS 55.0%
AGCGCTATGGATCCTGGTAG+TGG + chr2_4:2371823-2371842 Msa0308220:CDS 55.0%
!! GCTATGGATCCTGGTAGTGG+AGG + chr2_4:2371826-2371845 Msa0308220:CDS 55.0%
GATGCTGTGGCTCAACTTGG+TGG + chr2_4:2371928-2371947 Msa0308220:CDS 55.0%
GGGTGTGGCACCTAAGGAAA+AGG - chr2_4:2372135-2372154 None:intergenic 55.0%
! TCCTTAGGTGCCACACCCAA+AGG + chr2_4:2372137-2372156 Msa0308220:intron 55.0%
ACCTTTGGGTGTGGCACCTA+AGG - chr2_4:2372141-2372160 None:intergenic 55.0%
TCTGAGGACACCTTTGGGTG+TGG - chr2_4:2372150-2372169 None:intergenic 55.0%
GTACCATCGGATGAGCAGTC+AGG - chr2_4:2372334-2372353 None:intergenic 55.0%
CAATGCCTCAAACCCGTTGG+TGG + chr2_4:2372744-2372763 Msa0308220:intron 55.0%
GAAGAGCAACAGCGACTCAG+TGG + chr2_4:2373262-2373281 Msa0308220:CDS 55.0%
AGAGCAACAGCGACTCAGTG+GGG + chr2_4:2373264-2373283 Msa0308220:CDS 55.0%
!! GGGGTTCTTTCAGAAGCACC+CGG + chr2_4:2373283-2373302 Msa0308220:CDS 55.0%
!! TGGTGCTGATACACCAGAGC+CGG - chr2_4:2373305-2373324 None:intergenic 55.0%
GGTGTATCAGCACCAACTCC+AGG + chr2_4:2373310-2373329 Msa0308220:CDS 55.0%
! TTTTCTGGGTCAGGGGTTGC+CGG - chr2_4:2373373-2373392 None:intergenic 55.0%
!! CCGACTTGGCAACCAGAATC+TGG - chr2_4:2373469-2373488 None:intergenic 55.0%
TGTCAGCAGGAGAACCGACT+TGG - chr2_4:2373483-2373502 None:intergenic 55.0%
TCGCCATTAGGACTGTCAGC+AGG - chr2_4:2373496-2373515 None:intergenic 55.0%
! GCGGTTGGTTGCTGGTATGA+TGG - chr2_4:2373610-2373629 None:intergenic 55.0%
! GGAAAACAGCGGTTGGTTGC+TGG - chr2_4:2373618-2373637 None:intergenic 55.0%
CGGGTAAGGAAAACAGCGGT+TGG - chr2_4:2373625-2373644 None:intergenic 55.0%
! TAGGGTTTGGGGTTTGGGGG+GGG + chr2_4:2370603-2370622 Msa0308220:intron 60.0%
ATGGGTGGATGGATGGGTGG+GGG - chr2_4:2371009-2371028 None:intergenic 60.0%
!! GGTAGGAGCGCTATGGATCC+TGG + chr2_4:2371817-2371836 Msa0308220:CDS 60.0%
!! CTTTCAGAAGCACCCGGCTC+TGG + chr2_4:2373289-2373308 Msa0308220:CDS 60.0%
!! GGTGCTGATACACCAGAGCC+GGG - chr2_4:2373304-2373323 None:intergenic 60.0%
! AGGGTTTGGGGTTTGGGGGG+GGG + chr2_4:2370604-2370623 Msa0308220:intron 65.0%
! GGGTTTGGGGTTTGGGGGGG+GGG + chr2_4:2370605-2370624 Msa0308220:intron 70.0%
! GGTTTGGGGTTTGGGGGGGG+GGG + chr2_4:2370606-2370625 Msa0308220:intron 70.0%
! GTTTGGGGTTTGGGGGGGGG+GGG + chr2_4:2370607-2370626 Msa0308220:intron 70.0%
! TTTGGGGTTTGGGGGGGGGG+GGG + chr2_4:2370608-2370627 Msa0308220:intron 70.0%
TTGGGGGGGGGGGGGACGAA+AGG + chr2_4:2370616-2370635 Msa0308220:intron 75.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 2369757 2373965 2369757 ID=Msa0308220;Name=Msa0308220
chr2_4 mRNA 2369757 2373965 2369757 ID=Msa0308220-mRNA-1;Parent=Msa0308220;Name=Msa0308220-mRNA-1;_AED=0.06;_eAED=0.06;_QI=151|1|1|1|0.83|0.71|7|242|330
chr2_4 exon 2369757 2369896 2369757 ID=Msa0308220-mRNA-1:exon:553;Parent=Msa0308220-mRNA-1
chr2_4 exon 2371728 2371958 2371728 ID=Msa0308220-mRNA-1:exon:554;Parent=Msa0308220-mRNA-1
chr2_4 exon 2372144 2372220 2372144 ID=Msa0308220-mRNA-1:exon:555;Parent=Msa0308220-mRNA-1
chr2_4 exon 2372307 2372349 2372307 ID=Msa0308220-mRNA-1:exon:556;Parent=Msa0308220-mRNA-1
chr2_4 exon 2372489 2372549 2372489 ID=Msa0308220-mRNA-1:exon:557;Parent=Msa0308220-mRNA-1
chr2_4 exon 2372863 2372932 2372863 ID=Msa0308220-mRNA-1:exon:558;Parent=Msa0308220-mRNA-1
chr2_4 exon 2373202 2373965 2373202 ID=Msa0308220-mRNA-1:exon:559;Parent=Msa0308220-mRNA-1
chr2_4 five_prime_UTR 2369757 2369896 2369757 ID=Msa0308220-mRNA-1:five_prime_utr;Parent=Msa0308220-mRNA-1
chr2_4 five_prime_UTR 2371728 2371738 2371728 ID=Msa0308220-mRNA-1:five_prime_utr;Parent=Msa0308220-mRNA-1
chr2_4 CDS 2371739 2371958 2371739 ID=Msa0308220-mRNA-1:cds;Parent=Msa0308220-mRNA-1
chr2_4 CDS 2372144 2372220 2372144 ID=Msa0308220-mRNA-1:cds;Parent=Msa0308220-mRNA-1
chr2_4 CDS 2372307 2372349 2372307 ID=Msa0308220-mRNA-1:cds;Parent=Msa0308220-mRNA-1
chr2_4 CDS 2372489 2372549 2372489 ID=Msa0308220-mRNA-1:cds;Parent=Msa0308220-mRNA-1
chr2_4 CDS 2372863 2372932 2372863 ID=Msa0308220-mRNA-1:cds;Parent=Msa0308220-mRNA-1
chr2_4 CDS 2373202 2373723 2373202 ID=Msa0308220-mRNA-1:cds;Parent=Msa0308220-mRNA-1
chr2_4 three_prime_UTR 2373724 2373965 2373724 ID=Msa0308220-mRNA-1:three_prime_utr;Parent=Msa0308220-mRNA-1
Gene Sequence

>Msa0308220

ATGTATCAGCCAACTAATGTTCCTGATTCGAGTTTAATTGGAAGCAATCCATTAGTTCATGGTCAGCACATAGAGTCTGGTAGGAGCGCTATGGATCCTGGTAGTGGAGGAAACAGTCTTGCCAACAACTCTAATCTCAACTCAAAGCAACGTTTGCGTTGGACACATGAGCTACATGAACGCTTTGTTGATGCTGTGGCTCAACTTGGTGGCCCCGATCGTGCCACACCCAAAGGTGTCCTCAGAGTTATGGGTGTACAAGGCTTAACAATTTACCATGTTAAGAGCCATTTGCAGAAATACCGACTAGCAAAATACTTACCTGACTGCTCATCCGATGAGGGGAAAAAGACTGACAAGAAAGAAACGGGGGATATGCTTTCCAATCTAGATGGTTCATCGGGGATGCAGATTACCGAAGCACTAAAGCTTCAAATGGAGGTACAGAAGCGACTACATGAACAATTGGAGGTGCAGAGACAGCTACAATTAAGGATAGAAGCTCAGGGAAAATATTTGAAAAAGATAATCGAAGAGCAACAGCGACTCAGTGGGGTTCTTTCAGAAGCACCCGGCTCTGGTGTATCAGCACCAACTCCAGGAGATATGTCCCAACAGGAGCTCGACAATAAGACTGAACCGGCAACCCCTGACCCAGAAAAAGCTGCTAAAGAGCATGCACCAGCAAAGAGTCTTTCAGCAGAATCTTTTTCATCTCACCATGAACCGATGACACCAGATTCTGGTTGCCAAGTCGGTTCTCCTGCTGACAGTCCTAATGGCGAGCGATCAACTAAAAAGCAACGAGTAAGCGTGGAAGAAGCGTATTTGAAATCAGACATGGTGCTTCCTCATCAGATACTGGAGTCAAGCATGCCATCATACCAGCAACCAACCGCTGTTTTCCTTACCCGAGATCACTTTGATCCTTCTTTAGGCATGTCTACAAGAAGTGGTGAGGAATTGGATAAGCTTGGTGGCAGGAATATGTGA
Protein sequence

>Msa0308220

MYQPTNVPDSSLIGSNPLVHGQHIESGRSAMDPGSGGNSLANNSNLNSKQRLRWTHELHERFVDAVAQLGGPDRATPKGVLRVMGVQGLTIYHVKSHLQKYRLAKYLPDCSSDEGKKTDKKETGDMLSNLDGSSGMQITEALKLQMEVQKRLHEQLEVQRQLQLRIEAQGKYLKKIIEEQQRLSGVLSEAPGSGVSAPTPGDMSQQELDNKTEPATPDPEKAAKEHAPAKSLSAESFSSHHEPMTPDSGCQVGSPADSPNGERSTKKQRVSVEEAYLKSDMVLPHQILESSMPSYQQPTAVFLTRDHFDPSLGMSTRSGEELDKLGGRNM*