AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0308200

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0308200 MtrunA17_Chr2g0291021 97.273 330 9 0 1 330 1 330 0.0 663
Msa0308200 MtrunA17_Chr7g0254981 73.050 141 37 1 49 188 50 190 8.39e-69 216
Msa0308200 MtrunA17_Chr7g0261911 60.390 154 52 3 33 186 252 396 6.97e-53 181
Msa0308200 MtrunA17_Chr1g0175091 48.087 183 77 3 35 217 244 408 1.95e-51 176
Msa0308200 MtrunA17_Chr1g0190301 53.216 171 73 3 33 200 156 322 1.24e-48 167
Msa0308200 MtrunA17_Chr7g0258551 51.351 148 69 2 46 193 209 353 2.99e-47 164
Msa0308200 MtrunA17_Chr7g0274471 53.333 150 68 1 48 197 217 364 1.35e-46 163
Msa0308200 MtrunA17_Chr8g0356501 59.398 133 51 2 49 179 37 168 4.32e-44 154
Msa0308200 MtrunA17_Chr5g0409731 52.448 143 58 1 49 181 25 167 6.24e-44 152
Msa0308200 MtrunA17_Chr4g0042451 44.792 192 80 6 47 220 15 198 2.14e-43 152
Msa0308200 MtrunA17_Chr2g0310011 58.779 131 47 2 49 175 35 162 4.51e-42 149
Msa0308200 MtrunA17_Chr7g0242521 46.497 157 75 4 49 200 13 165 5.75e-41 143
Msa0308200 MtrunA17_Chr5g0417521 44.262 183 87 5 47 217 13 192 7.39e-41 146
Msa0308200 MtrunA17_Chr2g0321461 49.682 157 61 3 47 185 43 199 9.79e-41 146
Msa0308200 MtrunA17_Chr6g0461641 48.701 154 63 2 47 185 40 192 1.19e-40 146
Msa0308200 MtrunA17_Chr2g0290991 47.826 138 56 2 49 173 39 173 2.40e-34 127
Msa0308200 MtrunA17_Chr1g0197171 47.059 136 63 2 49 176 44 178 7.00e-33 123
Msa0308200 MtrunA17_Chr1g0175071 46.043 139 66 2 49 184 177 309 1.08e-30 118
Msa0308200 MtrunA17_Chr1g0175081 48.000 75 39 0 35 109 8 82 2.97e-22 89.7
Msa0308200 MtrunA17_Chr7g0258531 37.222 180 94 5 13 180 171 343 7.52e-22 94.7
Msa0308200 MtrunA17_Chr2g0288601 66.071 56 19 0 51 106 73 128 1.03e-16 75.5
Msa0308200 MtrunA17_Chr7g0243081 61.404 57 22 0 51 107 73 129 1.49e-16 79.3
Msa0308200 MtrunA17_Chr6g0479431 60.000 65 24 1 51 113 204 268 2.58e-16 79.0
Msa0308200 MtrunA17_Chr8g0366281 46.535 101 44 3 51 151 175 265 2.77e-16 78.6
Msa0308200 MtrunA17_Chr3g0095611 48.718 78 24 1 51 112 21 98 7.91e-16 75.1
Msa0308200 MtrunA17_Chr1g0198601 56.061 66 29 0 49 114 211 276 1.48e-15 76.6
Msa0308200 MtrunA17_Chr5g0410741 63.636 55 20 0 51 105 221 275 2.24e-15 76.3
Msa0308200 MtrunA17_Chr8g0379121 63.636 55 20 0 51 105 113 167 2.34e-15 76.6
Msa0308200 MtrunA17_Chr1g0162931 53.425 73 33 1 51 123 20 91 2.89e-15 75.1
Msa0308200 MtrunA17_Chr3g0109981 63.636 55 20 0 51 105 276 330 3.19e-15 76.3
Msa0308200 MtrunA17_Chr2g0320231 65.455 55 19 0 51 105 99 153 4.52e-15 75.5
Msa0308200 MtrunA17_Chr1g0154261 61.818 55 21 0 51 105 128 182 5.88e-15 74.3
Msa0308200 MtrunA17_Chr1g0210911 65.385 52 18 0 51 102 24 75 6.45e-15 74.7
Msa0308200 MtrunA17_Chr5g0402631 55.714 70 30 1 49 117 214 283 4.10e-14 72.4
Msa0308200 MtrunA17_Chr2g0283951 67.347 49 16 0 51 99 91 139 1.46e-13 70.9
Msa0308200 MtrunA17_Chr5g0422621 58.182 55 23 0 49 103 198 252 5.22e-13 68.9
Msa0308200 MtrunA17_Chr4g0062921 48.718 78 39 1 39 115 224 301 6.08e-13 69.3
Msa0308200 MtrunA17_Chr4g0009981 50.685 73 33 2 41 113 108 177 1.01e-12 67.4
Msa0308200 MtrunA17_Chr4g0045361 60.000 55 22 0 49 103 274 328 1.17e-12 68.2
Msa0308200 MtrunA17_Chr4g0009901 56.364 55 23 1 49 103 168 221 1.98e-12 66.6
Msa0308200 MtrunA17_Chr4g0031961 48.611 72 35 2 49 119 131 201 7.83e-12 65.1
Msa0308200 MtrunA17_Chr2g0324891 50.820 61 29 1 42 102 154 213 9.01e-12 65.5
Msa0308200 MtrunA17_Chr5g0400241 43.038 79 44 1 27 105 289 366 1.27e-11 65.5
Msa0308200 MtrunA17_Chr7g0255711 58.182 55 22 1 49 103 91 144 1.33e-11 64.3
Msa0308200 MtrunA17_Chr4g0009851 52.308 65 28 2 49 113 169 230 2.08e-11 63.9
Msa0308200 MtrunA17_Chr2g0304841 54.545 55 24 1 49 103 213 266 2.56e-11 64.7
Msa0308200 MtrunA17_Chr4g0067981 58.182 55 22 1 49 103 215 268 3.46e-11 64.3
Msa0308200 MtrunA17_Chr4g0040481 53.704 54 24 1 49 102 158 210 4.84e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0308200 AT2G01060.1 66.779 298 69 9 43 330 9 286 3.04e-119 345
Msa0308200 AT2G01060.2 62.646 257 66 9 84 330 1 237 1.80e-88 265
Msa0308200 AT5G29000.1 57.692 156 63 2 29 183 170 323 2.09e-51 174
Msa0308200 AT5G29000.3 57.692 156 63 2 29 183 170 323 2.09e-51 174
Msa0308200 AT5G29000.2 57.692 156 63 2 29 183 213 366 4.06e-51 175
Msa0308200 AT3G04450.4 52.273 176 76 3 44 212 193 367 7.56e-51 174
Msa0308200 AT3G04450.2 52.273 176 76 3 44 212 193 367 7.56e-51 174
Msa0308200 AT3G04450.1 52.273 176 76 3 44 212 233 407 1.34e-50 174
Msa0308200 AT4G28610.1 52.432 185 80 4 41 225 217 393 4.26e-50 172
Msa0308200 AT5G06800.1 57.534 146 57 3 37 182 182 322 6.49e-50 171
Msa0308200 AT4G13640.1 59.574 141 47 2 49 179 37 177 1.91e-49 167
Msa0308200 AT3G13040.3 50.265 189 86 4 44 227 236 421 1.98e-49 171
Msa0308200 AT3G13040.1 50.265 189 86 4 44 227 236 421 1.98e-49 171
Msa0308200 AT3G13040.2 50.265 189 86 4 44 227 236 421 1.98e-49 171
Msa0308200 AT3G24120.1 58.156 141 49 2 49 179 41 181 7.71e-49 166
Msa0308200 AT5G06800.2 55.705 149 60 3 37 182 182 327 7.97e-49 168
Msa0308200 AT1G79430.2 61.268 142 47 3 49 185 34 172 2.76e-48 166
Msa0308200 AT4G13640.2 58.333 144 47 3 49 179 37 180 3.08e-47 161
Msa0308200 AT3G24120.2 56.944 144 49 3 49 179 41 184 9.96e-47 160
Msa0308200 AT3G04030.3 48.333 180 72 4 23 185 19 194 8.16e-43 152
Msa0308200 AT1G69580.2 49.693 163 65 3 47 192 32 194 9.30e-43 151
Msa0308200 AT2G20400.2 53.165 158 71 2 49 206 231 385 1.04e-42 152
Msa0308200 AT1G69580.1 49.091 165 62 4 47 192 32 193 1.05e-42 150
Msa0308200 AT2G20400.1 53.797 158 69 2 49 206 231 384 1.33e-42 152
Msa0308200 AT5G18240.1 51.316 152 61 1 47 185 43 194 4.04e-42 150
Msa0308200 AT5G18240.4 51.316 152 61 1 47 185 43 194 4.04e-42 150
Msa0308200 AT3G04030.2 47.778 180 72 5 23 185 19 193 3.73e-40 145
Msa0308200 AT5G18240.5 50.000 152 61 2 47 185 43 192 7.33e-39 142
Msa0308200 AT5G45580.1 45.223 157 56 3 49 182 7 156 4.34e-37 134
Msa0308200 AT3G04030.1 45.556 180 71 5 23 185 19 188 6.82e-37 136
Msa0308200 AT5G45580.2 45.860 157 55 3 49 182 23 172 8.00e-37 134
Msa0308200 AT5G18240.2 48.026 152 60 2 47 185 43 188 3.20e-36 135
Msa0308200 AT5G18240.3 48.026 152 60 2 47 185 43 188 3.20e-36 135
Msa0308200 AT3G12730.1 47.826 138 64 4 49 179 23 159 3.09e-35 128
Msa0308200 AT1G79430.1 54.545 110 42 3 81 185 1 107 4.73e-27 108
Msa0308200 AT5G29000.4 60.674 89 33 1 29 117 213 299 3.09e-25 103
Msa0308200 AT3G04450.3 64.286 70 25 0 44 113 233 302 3.48e-25 103
Msa0308200 AT3G04030.5 49.153 118 47 2 81 185 1 118 1.34e-24 102
Msa0308200 AT5G06800.3 60.563 71 26 1 45 115 188 256 5.54e-24 99.8
Msa0308200 AT3G04030.4 44.915 118 46 3 81 185 1 112 4.54e-19 86.7
Msa0308200 AT5G18240.6 43.220 118 48 2 81 185 1 112 5.52e-19 86.7
Msa0308200 AT2G06020.1 59.649 57 23 0 49 105 86 142 7.10e-18 83.2
Msa0308200 AT2G42660.1 65.517 58 20 0 51 108 52 109 2.94e-17 80.5
Msa0308200 AT2G40260.1 57.333 75 20 1 51 125 84 146 3.08e-17 82.4
Msa0308200 AT4G04605.1 69.231 52 16 0 51 102 16 67 4.16e-17 79.3
Msa0308200 AT1G32240.1 43.396 106 51 2 51 156 215 311 1.79e-16 80.1
Msa0308200 AT1G32240.2 43.396 106 51 2 51 156 215 311 1.99e-16 79.7
Msa0308200 AT5G42630.2 57.576 66 28 0 51 116 107 172 4.46e-16 76.6
Msa0308200 AT5G42630.1 57.576 66 28 0 51 116 107 172 5.78e-16 77.4
Msa0308200 AT3G12730.2 39.623 106 56 4 81 179 1 105 1.29e-15 74.3
Msa0308200 AT5G16560.1 60.000 65 24 1 51 113 221 285 1.62e-15 77.4
Msa0308200 AT1G14600.1 69.231 52 16 0 51 102 25 76 2.03e-15 75.5
Msa0308200 AT4G17695.1 63.636 55 20 0 51 105 166 220 3.12e-15 75.9
Msa0308200 AT2G02060.2 69.231 52 16 0 51 102 31 82 3.28e-15 74.7
Msa0308200 AT2G02060.1 69.231 52 16 0 51 102 31 82 3.28e-15 74.7
Msa0308200 AT2G38300.1 63.636 55 20 0 51 105 56 110 3.76e-15 75.9
Msa0308200 AT4G37180.1 60.000 55 22 0 49 103 210 264 3.44e-14 73.2
Msa0308200 AT4G37180.2 60.000 55 22 0 49 103 217 271 4.20e-14 72.8
Msa0308200 AT4G04580.1 54.545 55 25 0 51 105 16 70 1.20e-13 68.6
Msa0308200 AT1G49560.1 54.545 66 30 0 49 114 192 257 1.52e-13 70.9
Msa0308200 AT3G25790.1 58.182 55 23 0 54 108 201 255 2.98e-13 70.1
Msa0308200 AT1G25550.1 55.385 65 29 0 44 108 204 268 5.27e-13 69.3
Msa0308200 AT3G10760.1 48.101 79 40 1 25 103 81 158 7.96e-13 68.9
Msa0308200 AT1G13300.1 44.086 93 41 2 16 108 159 240 1.40e-12 68.2
Msa0308200 AT1G68670.1 48.052 77 40 0 42 118 208 284 1.82e-12 67.8
Msa0308200 AT5G05090.1 60.377 53 20 1 51 103 83 134 4.25e-12 65.9
Msa0308200 AT4G37180.3 55.172 58 26 0 49 106 210 267 6.17e-12 65.5
Msa0308200 AT5G58080.1 52.174 69 28 3 49 117 194 257 7.07e-12 66.6
Msa0308200 AT5G59570.2 58.491 53 21 1 51 103 143 194 9.86e-12 65.1
Msa0308200 AT5G59570.1 58.491 53 21 1 51 103 143 194 9.86e-12 65.1
Msa0308200 AT3G46640.2 58.491 53 21 1 51 103 146 197 1.21e-11 65.1
Msa0308200 AT3G46640.1 58.491 53 21 1 51 103 146 197 1.21e-11 65.1
Msa0308200 AT3G46640.3 58.491 53 21 1 51 103 146 197 1.24e-11 65.1
Msa0308200 AT2G20570.1 51.471 68 31 2 35 102 140 205 1.35e-11 65.5
Msa0308200 AT2G03500.1 53.226 62 29 0 47 108 231 292 1.63e-11 65.5
Msa0308200 AT2G01760.3 45.333 75 38 2 49 123 135 206 1.66e-11 64.7
Msa0308200 AT5G44190.1 46.154 65 34 1 38 102 136 199 2.79e-11 64.3
Msa0308200 AT2G01760.1 45.333 75 38 2 49 123 200 271 2.96e-11 64.3
Msa0308200 AT5G44190.2 46.154 65 34 1 38 102 136 199 3.20e-11 63.9
Msa0308200 AT2G01760.2 45.333 75 38 2 49 123 228 299 4.22e-11 63.9
Msa0308200 AT2G40970.1 56.364 55 23 1 49 103 105 158 4.60e-11 62.8
Msa0308200 AT3G25790.2 58.696 46 19 0 54 99 201 246 9.79e-11 62.0

Find 99 sgRNAs with CRISPR-Local

Find 295 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCCTGATTCGAGTTTAAT+TGG 0.223090 2_4:+2335585 Msa0308200:CDS
CTCTAAAAGTAATTAAATTA+AGG 0.260834 2_4:-2337683 None:intergenic
GATCACTTTGATCCTTCTTT+AGG 0.297515 2_4:+2337474 Msa0308200:CDS
TTTAGAGAACTGATTTGAAT+TGG 0.300478 2_4:+2337699 Msa0308200:three_prime_UTR
TTAGTTGATCGCTCGCCATT+AGG 0.329748 2_4:-2337333 None:intergenic
CCATGTTAAGAGCCATTTGC+AGG 0.349218 2_4:+2336027 Msa0308200:CDS
TACAAGAAGTGGTGAGGAAT+TGG 0.349818 2_4:+2337503 Msa0308200:CDS
TGCAGAGACAGCTACAATTA+AGG 0.349960 2_4:+2337031 Msa0308200:CDS
TGGATTTCATAGATCTCATC+TGG 0.363640 2_4:+2333705 Msa0308200:five_prime_UTR
CTTCCAATTAAACTCGAATC+AGG 0.368180 2_4:-2335588 None:intergenic
CATAACTCTGAGGACACCTT+TGG 0.375653 2_4:-2335981 None:intergenic
CAAAGGTGTCCTCAGAGTTA+TGG 0.377183 2_4:+2335982 Msa0308200:CDS
ATGTGCTGACCATGAACTAA+TGG 0.385679 2_4:-2335615 None:intergenic
GAACCGATGACACCAGATTC+TGG 0.386395 2_4:+2337282 Msa0308200:CDS
AAGACTGACAAGAAAGAAAC+GGG 0.390608 2_4:+2336325 Msa0308200:CDS
TGAGTTGAGATTAGAGTTGT+TGG 0.390955 2_4:-2335688 None:intergenic
GGAAAACAGCGGTTGGTTGC+TGG 0.394253 2_4:-2337443 None:intergenic
AAAGGTGTCCTCAGAGTTAT+GGG 0.402741 2_4:+2335983 Msa0308200:CDS
ACAATTAAGGATAGAAGCTC+AGG 0.410929 2_4:+2337044 Msa0308200:CDS
GGAAGCAATCCATTAGTTCA+TGG 0.415844 2_4:+2335606 Msa0308200:CDS
TTAGAGAACTGATTTGAATT+GGG 0.421756 2_4:+2337700 Msa0308200:three_prime_UTR
CATTTGAAGCTTTAGTGCTT+CGG 0.424328 2_4:-2336705 None:intergenic
GATATGCTTTCCAATCTAGA+TGG 0.424452 2_4:+2336349 Msa0308200:CDS
TCCTGTTGGGACATATCTCC+TGG 0.428112 2_4:-2337156 None:intergenic
TTATATGCATTCTTAGTTGA+TGG 0.434188 2_4:+2337592 Msa0308200:three_prime_UTR
TCTGCTGAAAGACTCTTTGC+TGG 0.439579 2_4:-2337240 None:intergenic
GGTGCTTCCTCATCAGATAC+TGG 0.444138 2_4:+2337401 Msa0308200:CDS
CTTTCAGAAGCACCCGGCTC+TGG 0.447624 2_4:+2337117 Msa0308200:CDS
CCGACTTGGCAACCAGAATC+TGG 0.448662 2_4:-2337294 None:intergenic
CTCGAATCAGGAACATTAGT+TGG 0.450860 2_4:-2335576 None:intergenic
TCTCCTGCTGACAGTCCTAA+TGG 0.454643 2_4:+2337318 Msa0308200:CDS
TCACGATGAACCATCTAGAT+TGG 0.455940 2_4:-2336359 None:intergenic
GTTGATGCTGTGGCTCAACT+TGG 0.466308 2_4:+2335753 Msa0308200:CDS
AAGAAGGATCAAAGTGATCT+CGG 0.468691 2_4:-2337470 None:intergenic
GAAGCGACTACATGAACAAT+TGG 0.469698 2_4:+2336736 Msa0308200:CDS
CGAAGCACTAAAGCTTCAAA+TGG 0.471790 2_4:+2336706 Msa0308200:CDS
TCCAGGAGATATGTCCCAAC+AGG 0.476349 2_4:+2337155 Msa0308200:CDS
ATAACTCTGAGGACACCTTT+GGG 0.476739 2_4:-2335980 None:intergenic
ATTAAATTGGTAGCCCAAGA+TGG 0.479332 2_4:-2337778 None:intergenic
TCTTATTGTCGAGCTCCTGT+TGG 0.481895 2_4:-2337170 None:intergenic
CTTATTGTCGAGCTCCTGTT+GGG 0.485642 2_4:-2337169 None:intergenic
CTCAGAGTTATGGGTGTACA+AGG 0.485843 2_4:+2335992 Msa0308200:CDS
GGTAGGAGCGCTATGGATCC+TGG 0.493466 2_4:+2335645 Msa0308200:CDS
CTACACAGCTCCGAAATTGA+AGG 0.498072 2_4:+2333658 Msa0308200:five_prime_UTR
CATGGTCAGCACATAGAGTC+TGG 0.500025 2_4:+2335624 Msa0308200:CDS
GAGTGCACATTACCTGCAAA+TGG 0.500488 2_4:-2336039 None:intergenic
AAAGACTGACAAGAAAGAAA+CGG 0.503951 2_4:+2336324 Msa0308200:CDS
TCCTTAGGTGCCACACCCAA+AGG 0.508503 2_4:+2335965 Msa0308200:intron
GATGCTGTGGCTCAACTTGG+TGG 0.512420 2_4:+2335756 Msa0308200:CDS
GCTCGACAATAAGACTGAAC+CGG 0.517717 2_4:+2337179 Msa0308200:CDS
TGGTGCTGATACACCAGAGC+CGG 0.522258 2_4:-2337130 None:intergenic
AATCTGGTGTCATCGGTTCA+TGG 0.523061 2_4:-2337278 None:intergenic
GATCAAAGTGATCTCGGGTA+AGG 0.525714 2_4:-2337464 None:intergenic
CCTGCAAATGGCTCTTAACA+TGG 0.525821 2_4:-2336027 None:intergenic
CCAGATTCTGGTTGCCAAGT+CGG 0.528854 2_4:+2337294 Msa0308200:CDS
GTACCATCGGATGAGCAGTC+AGG 0.531157 2_4:-2336159 None:intergenic
ACTCAAAGCAACGTTTGCGT+TGG 0.531602 2_4:+2335706 Msa0308200:CDS
AGAAGGATCAAAGTGATCTC+GGG 0.531953 2_4:-2337469 None:intergenic
GGTGTATCAGCACCAACTCC+AGG 0.533448 2_4:+2337138 Msa0308200:CDS
AGCGCTATGGATCCTGGTAG+TGG 0.534994 2_4:+2335651 Msa0308200:CDS
CTTGTAGACATGCCTAAAGA+AGG 0.541592 2_4:-2337486 None:intergenic
TTAGGCATGTCTACAAGAAG+TGG 0.546196 2_4:+2337492 Msa0308200:CDS
CGGGTAAGGAAAACAGCGGT+TGG 0.546421 2_4:-2337450 None:intergenic
GGTGCTGATACACCAGAGCC+GGG 0.552630 2_4:-2337129 None:intergenic
TCTGAGGACACCTTTGGGTG+TGG 0.554653 2_4:-2335975 None:intergenic
CAACCAGAATCTGGTGTCAT+CGG 0.558599 2_4:-2337285 None:intergenic
AGAGTCTGGTAGGAGCGCTA+TGG 0.562642 2_4:+2335638 Msa0308200:CDS
GCGGTTGGTTGCTGGTATGA+TGG 0.564870 2_4:-2337435 None:intergenic
TGTCAGCAGGAGAACCGACT+TGG 0.567410 2_4:-2337308 None:intergenic
AATTGGATAAGCTTGGTGGC+AGG 0.568213 2_4:+2337520 Msa0308200:CDS
TGGGACATATCTCCTGGAGT+TGG 0.568536 2_4:-2337150 None:intergenic
TTAAATTGGTAGCCCAAGAT+GGG 0.568543 2_4:-2337777 None:intergenic
AGACTGTTTCCTCCACTACC+AGG 0.568775 2_4:-2335663 None:intergenic
AAGAGCAACAGCGACTCAGT+GGG 0.575024 2_4:+2337091 Msa0308200:CDS
TACACAGCTCCGAAATTGAA+GGG 0.581590 2_4:+2333659 Msa0308200:five_prime_UTR
TATGCATTCTTAGTTGATGG+AGG 0.586098 2_4:+2337595 Msa0308200:three_prime_UTR
TCGCCATTAGGACTGTCAGC+AGG 0.590479 2_4:-2337321 None:intergenic
GGTGAGGAATTGGATAAGCT+TGG 0.592014 2_4:+2337513 Msa0308200:CDS
TTACCTGACTGCTCATCCGA+TGG 0.595863 2_4:+2336156 Msa0308200:CDS
GCGACTACATGAACAATTGG+AGG 0.598747 2_4:+2336739 Msa0308200:CDS
TGAACGCTTTGTTGATGCTG+TGG 0.601507 2_4:+2335743 Msa0308200:CDS
GGGGTTCTTTCAGAAGCACC+CGG 0.619430 2_4:+2337111 Msa0308200:CDS
GGATTTCATAGATCTCATCT+GGG 0.628659 2_4:+2333706 Msa0308200:five_prime_UTR
GTCAGCACATAGAGTCTGGT+AGG 0.630464 2_4:+2335628 Msa0308200:CDS
AGCACTAAAGCTTCAAATGG+AGG 0.636135 2_4:+2336709 Msa0308200:CDS
GAAGAGCAACAGCGACTCAG+TGG 0.642615 2_4:+2337090 Msa0308200:CDS
CTTGACTCCAGTATCTGATG+AGG 0.648174 2_4:-2337408 None:intergenic
CATGTCTACAAGAAGTGGTG+AGG 0.650625 2_4:+2337497 Msa0308200:CDS
GAGGAATTGGATAAGCTTGG+TGG 0.651592 2_4:+2337516 Msa0308200:CDS
CAATTAAGGATAGAAGCTCA+GGG 0.654933 2_4:+2337045 Msa0308200:CDS
GGGGAAAATACTCACGATCG+GGG 0.658151 2_4:-2335779 None:intergenic
GCTATGGATCCTGGTAGTGG+AGG 0.662575 2_4:+2335654 Msa0308200:CDS
ACACAGCTCCGAAATTGAAG+GGG 0.667923 2_4:+2333660 Msa0308200:five_prime_UTR
GACTGACAAGAAAGAAACGG+GGG 0.678708 2_4:+2336327 Msa0308200:CDS
AGCGTATTTGAAATCAGACA+TGG 0.689566 2_4:+2337380 Msa0308200:CDS
AGAGCAACAGCGACTCAGTG+GGG 0.711050 2_4:+2337092 Msa0308200:CDS
CTTGTACACCCATAACTCTG+AGG 0.715783 2_4:-2335991 None:intergenic
ATCTCGGGTAAGGAAAACAG+CGG 0.741797 2_4:-2337454 None:intergenic
AGACTGACAAGAAAGAAACG+GGG 0.753105 2_4:+2336326 Msa0308200:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAAATAAATAAATGTTAAT+TGG + chr2_4:2334132-2334151 Msa0308200:intron 10.0%
!!! TTTTATTAATTTCAATCAAA+TGG + chr2_4:2335215-2335234 Msa0308200:intron 10.0%
!!! TTGTATTGTTTTTACATTTT+TGG + chr2_4:2333841-2333860 Msa0308200:intron 15.0%
!!! AAAGTTTGATTTTTAAGTTT+TGG + chr2_4:2333928-2333947 Msa0308200:intron 15.0%
!! ATACAATATTAACAACAATT+AGG - chr2_4:2334107-2334126 None:intergenic 15.0%
!! ATAAATAAATGTTAATTGGT+TGG + chr2_4:2334136-2334155 Msa0308200:intron 15.0%
!! ATTAAATTTATGATCTATTG+TGG + chr2_4:2335026-2335045 Msa0308200:intron 15.0%
!!! GATTTAAAAACTTTTAGATA+AGG - chr2_4:2335932-2335951 None:intergenic 15.0%
!! CATTAAAAAAACAAGAATAA+TGG - chr2_4:2336086-2336105 None:intergenic 15.0%
!!! TAAAAAGTTATGTTTCTTTT+AGG + chr2_4:2336545-2336564 Msa0308200:intron 15.0%
!!! TTTTATTTTGAATCAAGTAA+AGG + chr2_4:2336934-2336953 Msa0308200:intron 15.0%
!!! CTCTAAAAGTAATTAAATTA+AGG - chr2_4:2337686-2337705 None:intergenic 15.0%
!!! AAAGCATTCATTGATTTTTA+GGG + chr2_4:2334235-2334254 Msa0308200:intron 20.0%
!! ATCACTAAGATAACATATAT+AGG - chr2_4:2334363-2334382 None:intergenic 20.0%
!!! AGTAATTTGATGATATTTGA+AGG + chr2_4:2334521-2334540 Msa0308200:intron 20.0%
!!! GTAATTTGATGATATTTGAA+GGG + chr2_4:2334522-2334541 Msa0308200:intron 20.0%
!!! TAATTTGATGATATTTGAAG+GGG + chr2_4:2334523-2334542 Msa0308200:intron 20.0%
!!! CTTTTTTTAATGACAAATGT+TGG + chr2_4:2334732-2334751 Msa0308200:intron 20.0%
!!! ACAGTTTGAAAAGTTTTTTA+TGG + chr2_4:2335320-2335339 Msa0308200:intron 20.0%
!! AAATCAGTAAGATCTAAATA+TGG - chr2_4:2336208-2336227 None:intergenic 20.0%
!! AGAATGCTTAATACTTTAAA+CGG - chr2_4:2336243-2336262 None:intergenic 20.0%
!! AAAGTTATCAGCAAATTTAA+AGG - chr2_4:2336282-2336301 None:intergenic 20.0%
!!! ACTTTTTAATTGTCTTATAG+AGG + chr2_4:2336297-2336316 Msa0308200:intron 20.0%
!!! CTTTTTAATTGTCTTATAGA+GGG + chr2_4:2336298-2336317 Msa0308200:intron 20.0%
!!! TTTTTAATTGTCTTATAGAG+GGG + chr2_4:2336299-2336318 Msa0308200:intron 20.0%
!! TATCATAATTATACAAGGAA+TGG + chr2_4:2336409-2336428 Msa0308200:intron 20.0%
!! TAGAAAAAATAACAGTTACA+AGG - chr2_4:2337000-2337019 None:intergenic 20.0%
!!! TTATTTTTTCTATGTGAAAC+AGG + chr2_4:2337008-2337027 Msa0308200:intron 20.0%
!! GAAAATTTTGTATACGTAGT+TGG + chr2_4:2333685-2333704 Msa0308200:five_prime_UTR 25.0%
! TTATAGAAATGAAGAAGAGA+AGG - chr2_4:2333776-2333795 None:intergenic 25.0%
! GAGAAAGTAAGGAATAAAAA+GGG - chr2_4:2333799-2333818 None:intergenic 25.0%
! AGAGAAAGTAAGGAATAAAA+AGG - chr2_4:2333800-2333819 None:intergenic 25.0%
! TAAAACCAAATAAGCCAAAA+GGG - chr2_4:2333870-2333889 None:intergenic 25.0%
! GCTTTCAAAAAGTTAAAACA+AGG - chr2_4:2334220-2334239 None:intergenic 25.0%
!!! GAAAGCATTCATTGATTTTT+AGG + chr2_4:2334234-2334253 Msa0308200:intron 25.0%
! GATGTTAATGTGATGAAAAA+TGG + chr2_4:2334276-2334295 Msa0308200:intron 25.0%
! TGTTATCTTAGTGATTAATC+AGG + chr2_4:2334368-2334387 Msa0308200:intron 25.0%
!!! ATTTTCAACTCCACTTTTTA+GGG + chr2_4:2334415-2334434 Msa0308200:intron 25.0%
! TAGCAAAGAATCTATCTAAT+AGG - chr2_4:2334550-2334569 None:intergenic 25.0%
! AAATTGAATTCAACTTTCTG+TGG + chr2_4:2334629-2334648 Msa0308200:intron 25.0%
!! TAGTTTAAGTTTCTTAGACA+TGG + chr2_4:2334907-2334926 Msa0308200:intron 25.0%
!! GTGTTTGTTAAATACTGTAT+TGG + chr2_4:2335255-2335274 Msa0308200:intron 25.0%
! CGTTGAAAAAAATTGTACAA+CGG - chr2_4:2335300-2335319 None:intergenic 25.0%
! AACTGAATCTGATTAAGAAT+TGG + chr2_4:2335383-2335402 Msa0308200:intron 25.0%
! ATTTCAGCTAACGAAAAAAT+GGG - chr2_4:2335802-2335821 None:intergenic 25.0%
! TTGGTTACATCATACATATT+TGG + chr2_4:2335835-2335854 Msa0308200:intron 25.0%
!! CAGATATCAAATACTTTTGT+TGG + chr2_4:2335860-2335879 Msa0308200:intron 25.0%
! TAAATTCATCATCTAGCATT+AGG - chr2_4:2335894-2335913 None:intergenic 25.0%
! TGGGTTATCATAATTATACA+AGG + chr2_4:2336404-2336423 Msa0308200:intron 25.0%
!!! TATAGATTTTTTTCCACCAA+CGG - chr2_4:2336588-2336607 None:intergenic 25.0%
! TTATATGCATTCTTAGTTGA+TGG + chr2_4:2337592-2337611 Msa0308200:three_prime_UTR 25.0%
!!! TTTAGAGAACTGATTTGAAT+TGG + chr2_4:2337699-2337718 Msa0308200:three_prime_UTR 25.0%
!!! TTAGAGAACTGATTTGAATT+GGG + chr2_4:2337700-2337719 Msa0308200:three_prime_UTR 25.0%
! AAAAACTCAACCTTGAAAAT+GGG - chr2_4:2337754-2337773 None:intergenic 25.0%
!!! ATTTTCAAGGTTGAGTTTTT+GGG + chr2_4:2337754-2337773 Msa0308200:three_prime_UTR 25.0%
TGAAAGAAAGAGAGAAAGTA+AGG - chr2_4:2333810-2333829 None:intergenic 30.0%
CTAAAACCAAATAAGCCAAA+AGG - chr2_4:2333871-2333890 None:intergenic 30.0%
! TTTTCAGCTTTTGTTGATGT+GGG + chr2_4:2334042-2334061 Msa0308200:intron 30.0%
ATAGCTAACATCAGTGAATT+GGG - chr2_4:2334319-2334338 None:intergenic 30.0%
TATAGCTAACATCAGTGAAT+TGG - chr2_4:2334320-2334339 None:intergenic 30.0%
TTCACTGATGTTAGCTATAA+AGG + chr2_4:2334321-2334340 Msa0308200:intron 30.0%
!!! GATTTTCAACTCCACTTTTT+AGG + chr2_4:2334414-2334433 Msa0308200:intron 30.0%
TATCTAGAGATAGATGATGA+TGG - chr2_4:2334607-2334626 None:intergenic 30.0%
!!! TTGATTTTTTCTCCTTTGTC+CGG + chr2_4:2334770-2334789 Msa0308200:intron 30.0%
ACTTGGTAAACTTCTTGTAT+GGG - chr2_4:2334855-2334874 None:intergenic 30.0%
AACTTGGTAAACTTCTTGTA+TGG - chr2_4:2334856-2334875 None:intergenic 30.0%
TTCTAAGTCAAACTAGAACT+TGG - chr2_4:2334872-2334891 None:intergenic 30.0%
!! AGTTCTAGTTTGACTTAGAA+TGG + chr2_4:2334872-2334891 Msa0308200:intron 30.0%
AAACTATTATTAGTGAACCC+GGG - chr2_4:2335140-2335159 None:intergenic 30.0%
CAAACTATTATTAGTGAACC+CGG - chr2_4:2335141-2335160 None:intergenic 30.0%
ATTTCAATCAAATGGAGCAT+TGG + chr2_4:2335223-2335242 Msa0308200:intron 30.0%
AAATTGTACAACGGATCATT+TGG - chr2_4:2335291-2335310 None:intergenic 30.0%
AACTCTCTTTCTTTCAATCA+TGG - chr2_4:2335362-2335381 None:intergenic 30.0%
ATTGGAATTAGAACATGTTG+AGG + chr2_4:2335401-2335420 Msa0308200:intron 30.0%
TTGGAATTAGAACATGTTGA+GGG + chr2_4:2335402-2335421 Msa0308200:intron 30.0%
AGACAGAAACACACAAAAAT+TGG - chr2_4:2335429-2335448 None:intergenic 30.0%
!! AAAGGTTGGTAAAGAAATGA+GGG - chr2_4:2335471-2335490 None:intergenic 30.0%
!! AAAAGGTTGGTAAAGAAATG+AGG - chr2_4:2335472-2335491 None:intergenic 30.0%
ACTTAATTGCAGATCGTAAA+AGG - chr2_4:2335489-2335508 None:intergenic 30.0%
TTTCAGCTAACGAAAAAATG+GGG - chr2_4:2335801-2335820 None:intergenic 30.0%
CATTTCAGCTAACGAAAAAA+TGG - chr2_4:2335803-2335822 None:intergenic 30.0%
TGAAATGTAATGCGTAGAAT+TGG + chr2_4:2335816-2335835 Msa0308200:intron 30.0%
TGCTAGATGATGAATTTATC+AGG + chr2_4:2335896-2335915 Msa0308200:intron 30.0%
! TCTATACTATCCTTTTCCTT+AGG + chr2_4:2335950-2335969 Msa0308200:intron 30.0%
!! ATAATGGATACCACATTAAG+AGG - chr2_4:2336070-2336089 None:intergenic 30.0%
ATTTAAAGGAGGAAACAAAG+TGG - chr2_4:2336268-2336287 None:intergenic 30.0%
GTTATCAGCAAATTTAAAGG+AGG - chr2_4:2336279-2336298 None:intergenic 30.0%
AAAGACTGACAAGAAAGAAA+CGG + chr2_4:2336324-2336343 Msa0308200:CDS 30.0%
TGATAACCCAAAAGCAAAAA+GGG - chr2_4:2336394-2336413 None:intergenic 30.0%
ATGATAACCCAAAAGCAAAA+AGG - chr2_4:2336395-2336414 None:intergenic 30.0%
CTAAATAGAACCATCTCATT+TGG + chr2_4:2336481-2336500 Msa0308200:intron 30.0%
!!! ATAGATTTTTTTCCACCAAC+GGG - chr2_4:2336587-2336606 None:intergenic 30.0%
TAATAATGTTACATGTCTGC+AGG + chr2_4:2336669-2336688 Msa0308200:intron 30.0%
AATAATGTTACATGTCTGCA+GGG + chr2_4:2336670-2336689 Msa0308200:intron 30.0%
GTGAGATGAAATGATGATAT+TGG + chr2_4:2336849-2336868 Msa0308200:intron 30.0%
AAGGAATAATAGACAGAATG+AGG + chr2_4:2336953-2336972 Msa0308200:intron 30.0%
AGGAATAATAGACAGAATGA+GGG + chr2_4:2336954-2336973 Msa0308200:intron 30.0%
CAAAAACTCAACCTTGAAAA+TGG - chr2_4:2337755-2337774 None:intergenic 30.0%
!! CATTTTCAAGGTTGAGTTTT+TGG + chr2_4:2337753-2337772 Msa0308200:three_prime_UTR 30.0%
! ATTTTCGTCCCCTTCAATTT+CGG - chr2_4:2333671-2333690 None:intergenic 35.0%
TGGATTTCATAGATCTCATC+TGG + chr2_4:2333705-2333724 Msa0308200:five_prime_UTR 35.0%
GGATTTCATAGATCTCATCT+GGG + chr2_4:2333706-2333725 Msa0308200:five_prime_UTR 35.0%
!!! TACATTTTTGGTGACCCTTT+TGG + chr2_4:2333853-2333872 Msa0308200:intron 35.0%
AGCACAAAACTACACAAGAA+GGG - chr2_4:2333974-2333993 None:intergenic 35.0%
! CTTTTCAGCTTTTGTTGATG+TGG + chr2_4:2334041-2334060 Msa0308200:intron 35.0%
! TTTCAGCTTTTGTTGATGTG+GGG + chr2_4:2334043-2334062 Msa0308200:intron 35.0%
AAAACCCATAACACTCATCA+TGG - chr2_4:2334192-2334211 None:intergenic 35.0%
ACTGATGTTAGCTATAAAGG+TGG + chr2_4:2334324-2334343 Msa0308200:intron 35.0%
!!! AACTCCACTTTTTAGGGTTT+GGG + chr2_4:2334421-2334440 Msa0308200:intron 35.0%
!!! ACTTTTTAGGGTTTGGGTTT+GGG + chr2_4:2334427-2334446 Msa0308200:intron 35.0%
!!! TGATTTTTTCTCCTTTGTCC+GGG + chr2_4:2334771-2334790 Msa0308200:intron 35.0%
!! TCAATTGGTTTGACCTAAGA+AGG - chr2_4:2334810-2334829 None:intergenic 35.0%
TTTCCTAGAGATACAAACGA+AGG + chr2_4:2334962-2334981 Msa0308200:intron 35.0%
!!! AAATGGAGCATTGGTTTTCT+GGG + chr2_4:2335232-2335251 Msa0308200:intron 35.0%
TGGAATTAGAACATGTTGAG+GGG + chr2_4:2335403-2335422 Msa0308200:intron 35.0%
!! AAGGTTGGTAAAGAAATGAG+GGG - chr2_4:2335470-2335489 None:intergenic 35.0%
AATTGCAGATCGTAAAAGGT+TGG - chr2_4:2335485-2335504 None:intergenic 35.0%
! GTTCCTGATTCGAGTTTAAT+TGG + chr2_4:2335585-2335604 Msa0308200:CDS 35.0%
CTTCCAATTAAACTCGAATC+AGG - chr2_4:2335591-2335610 None:intergenic 35.0%
! TGAGTTGAGATTAGAGTTGT+TGG - chr2_4:2335691-2335710 None:intergenic 35.0%
! CAGGTAAGTATTTTGCTAGT+CGG - chr2_4:2336143-2336162 None:intergenic 35.0%
AAATCAGATTGACGTACCAT+CGG - chr2_4:2336175-2336194 None:intergenic 35.0%
AAGACTGACAAGAAAGAAAC+GGG + chr2_4:2336325-2336344 Msa0308200:CDS 35.0%
GATATGCTTTCCAATCTAGA+TGG + chr2_4:2336349-2336368 Msa0308200:CDS 35.0%
! ATAGCTTCCCTTTTTGCTTT+TGG + chr2_4:2336384-2336403 Msa0308200:intron 35.0%
!! TAGCTTCCCTTTTTGCTTTT+GGG + chr2_4:2336385-2336404 Msa0308200:intron 35.0%
! GAGATGGTTCTATTTAGTTC+AGG - chr2_4:2336478-2336497 None:intergenic 35.0%
GGATGTAAAACCAAATGAGA+TGG - chr2_4:2336494-2336513 None:intergenic 35.0%
!!! TCTCATTTGGTTTTACATCC+TGG + chr2_4:2336494-2336513 Msa0308200:intron 35.0%
ATAATGTTACATGTCTGCAG+GGG + chr2_4:2336671-2336690 Msa0308200:intron 35.0%
! CATTTGAAGCTTTAGTGCTT+CGG - chr2_4:2336708-2336727 None:intergenic 35.0%
ACAATTAAGGATAGAAGCTC+AGG + chr2_4:2337044-2337063 Msa0308200:CDS 35.0%
CAATTAAGGATAGAAGCTCA+GGG + chr2_4:2337045-2337064 Msa0308200:CDS 35.0%
! AGCGTATTTGAAATCAGACA+TGG + chr2_4:2337380-2337399 Msa0308200:CDS 35.0%
AAGAAGGATCAAAGTGATCT+CGG - chr2_4:2337473-2337492 None:intergenic 35.0%
GATCACTTTGATCCTTCTTT+AGG + chr2_4:2337474-2337493 Msa0308200:CDS 35.0%
! TGTGATTTTGTGTAATCTGC+AGG + chr2_4:2337547-2337566 Msa0308200:exon 35.0%
! GTGATTTTGTGTAATCTGCA+GGG + chr2_4:2337548-2337567 Msa0308200:exon 35.0%
!! TATGCATTCTTAGTTGATGG+AGG + chr2_4:2337595-2337614 Msa0308200:three_prime_UTR 35.0%
! GTCATATGTTCCCATTTTCA+AGG + chr2_4:2337741-2337760 Msa0308200:three_prime_UTR 35.0%
TACACAGCTCCGAAATTGAA+GGG + chr2_4:2333659-2333678 Msa0308200:five_prime_UTR 40.0%
CAGCACAAAACTACACAAGA+AGG - chr2_4:2333975-2333994 None:intergenic 40.0%
!!! TGTGTAGTTTTGTGCTGTGT+TGG + chr2_4:2333978-2333997 Msa0308200:intron 40.0%
! TTCAGCTTTTGTTGATGTGG+GGG + chr2_4:2334044-2334063 Msa0308200:intron 40.0%
!!! TTGCTGGGTTTTTTCTGTTG+TGG + chr2_4:2334072-2334091 Msa0308200:intron 40.0%
!!! TGCTGGGTTTTTTCTGTTGT+GGG + chr2_4:2334073-2334092 Msa0308200:intron 40.0%
TTCAGCCATGATGAGTGTTA+TGG + chr2_4:2334184-2334203 Msa0308200:intron 40.0%
TCAGCCATGATGAGTGTTAT+GGG + chr2_4:2334185-2334204 Msa0308200:intron 40.0%
!!! CAACTCCACTTTTTAGGGTT+TGG + chr2_4:2334420-2334439 Msa0308200:intron 40.0%
CAAACCCAAACCCTAAAAAG+TGG - chr2_4:2334428-2334447 None:intergenic 40.0%
!!! CACTTTTTAGGGTTTGGGTT+TGG + chr2_4:2334426-2334445 Msa0308200:intron 40.0%
!!! CTTTTTAGGGTTTGGGTTTG+GGG + chr2_4:2334428-2334447 Msa0308200:intron 40.0%
!!! TTTTTAGGGTTTGGGTTTGG+GGG + chr2_4:2334429-2334448 Msa0308200:intron 40.0%
ACGAAAGGTTACCTATGCTA+GGG + chr2_4:2334459-2334478 Msa0308200:intron 40.0%
TAACATCCTTTCCCTAGCAT+AGG - chr2_4:2334473-2334492 None:intergenic 40.0%
TTTGAACCCAAGACCTTCTT+AGG + chr2_4:2334794-2334813 Msa0308200:intron 40.0%
GTTTGACCTAAGAAGGTCTT+GGG - chr2_4:2334803-2334822 None:intergenic 40.0%
AAACTTCTTGTATGGGTGGA+TGG - chr2_4:2334848-2334867 None:intergenic 40.0%
TGGTAAACTTCTTGTATGGG+TGG - chr2_4:2334852-2334871 None:intergenic 40.0%
AAAGTCAAGCCTCATGGAAT+AGG - chr2_4:2334943-2334962 None:intergenic 40.0%
TAGGAAAAAGTCAAGCCTCA+TGG - chr2_4:2334949-2334968 None:intergenic 40.0%
CATCCTTCGTTTGTATCTCT+AGG - chr2_4:2334968-2334987 None:intergenic 40.0%
!!! CAAATGGAGCATTGGTTTTC+TGG + chr2_4:2335231-2335250 Msa0308200:intron 40.0%
!!! AATGGAGCATTGGTTTTCTG+GGG + chr2_4:2335233-2335252 Msa0308200:intron 40.0%
CTCGAATCAGGAACATTAGT+TGG - chr2_4:2335579-2335598 None:intergenic 40.0%
GGAAGCAATCCATTAGTTCA+TGG + chr2_4:2335606-2335625 Msa0308200:CDS 40.0%
! ATGTGCTGACCATGAACTAA+TGG - chr2_4:2335618-2335637 None:intergenic 40.0%
ATGGGGAAAATACTCACGAT+CGG - chr2_4:2335784-2335803 None:intergenic 40.0%
! ATAACTCTGAGGACACCTTT+GGG - chr2_4:2335983-2336002 None:intergenic 40.0%
!! AAAGGTGTCCTCAGAGTTAT+GGG + chr2_4:2335983-2336002 Msa0308200:CDS 40.0%
! TGCTAGTCGGTATTTCTGTA+TGG - chr2_4:2336130-2336149 None:intergenic 40.0%
AGACTGACAAGAAAGAAACG+GGG + chr2_4:2336326-2336345 Msa0308200:CDS 40.0%
TCACGATGAACCATCTAGAT+TGG - chr2_4:2336362-2336381 None:intergenic 40.0%
ACTAGTGCAAATTTGACTCC+AGG - chr2_4:2336515-2336534 None:intergenic 40.0%
TAATGTTACATGTCTGCAGG+GGG + chr2_4:2336672-2336691 Msa0308200:intron 40.0%
! CGAAGCACTAAAGCTTCAAA+TGG + chr2_4:2336706-2336725 Msa0308200:CDS 40.0%
AGCACTAAAGCTTCAAATGG+AGG + chr2_4:2336709-2336728 Msa0308200:CDS 40.0%
GAAGCGACTACATGAACAAT+TGG + chr2_4:2336736-2336755 Msa0308200:CDS 40.0%
CAGATCAGATCAGTTCAACA+AGG - chr2_4:2336772-2336791 None:intergenic 40.0%
TGCAGAGACAGCTACAATTA+AGG + chr2_4:2337031-2337050 Msa0308200:CDS 40.0%
!! GCTCTTTAGCAGCTTTTTCT+GGG - chr2_4:2337215-2337234 None:intergenic 40.0%
!! TGCTCTTTAGCAGCTTTTTC+TGG - chr2_4:2337216-2337235 None:intergenic 40.0%
TAAAAAGCAACGAGTAAGCG+TGG + chr2_4:2337353-2337372 Msa0308200:CDS 40.0%
AGAAGGATCAAAGTGATCTC+GGG - chr2_4:2337472-2337491 None:intergenic 40.0%
CTTGTAGACATGCCTAAAGA+AGG - chr2_4:2337489-2337508 None:intergenic 40.0%
TTAGGCATGTCTACAAGAAG+TGG + chr2_4:2337492-2337511 Msa0308200:CDS 40.0%
TACAAGAAGTGGTGAGGAAT+TGG + chr2_4:2337503-2337522 Msa0308200:CDS 40.0%
CTACACAGCTCCGAAATTGA+AGG + chr2_4:2333658-2333677 Msa0308200:five_prime_UTR 45.0%
ACACAGCTCCGAAATTGAAG+GGG + chr2_4:2333660-2333679 Msa0308200:five_prime_UTR 45.0%
!!! GGTGACCCTTTTGGCTTATT+TGG + chr2_4:2333862-2333881 Msa0308200:intron 45.0%
!!! TTTTAGGGTTTGGGTTTGGG+GGG + chr2_4:2334430-2334449 Msa0308200:intron 45.0%
GACGAAAGGTTACCTATGCT+AGG + chr2_4:2334458-2334477 Msa0308200:intron 45.0%
AGGTTACCTATGCTAGGGAA+AGG + chr2_4:2334464-2334483 Msa0308200:intron 45.0%
! GCTAGCAGCATCAACGATTT+TGG - chr2_4:2334580-2334599 None:intergenic 45.0%
GGTTTGACCTAAGAAGGTCT+TGG - chr2_4:2334804-2334823 None:intergenic 45.0%
TCTTGTATGGGTGGATGGAT+GGG - chr2_4:2334843-2334862 None:intergenic 45.0%
TTCTTGTATGGGTGGATGGA+TGG - chr2_4:2334844-2334863 None:intergenic 45.0%
AACAGTGTGCCTATTCCATG+AGG + chr2_4:2334931-2334950 Msa0308200:intron 45.0%
! TTTAGACCCATGCTTTTGCC+CGG + chr2_4:2335119-2335138 Msa0308200:intron 45.0%
TGCTGTGTGCAGAAAGTCAT+TGG + chr2_4:2335185-2335204 Msa0308200:intron 45.0%
ACTCAAAGCAACGTTTGCGT+TGG + chr2_4:2335706-2335725 Msa0308200:CDS 45.0%
! TGAACGCTTTGTTGATGCTG+TGG + chr2_4:2335743-2335762 Msa0308200:CDS 45.0%
TGGGGAAAATACTCACGATC+GGG - chr2_4:2335783-2335802 None:intergenic 45.0%
! CATAACTCTGAGGACACCTT+TGG - chr2_4:2335984-2336003 None:intergenic 45.0%
!! CAAAGGTGTCCTCAGAGTTA+TGG + chr2_4:2335982-2336001 Msa0308200:CDS 45.0%
CTTGTACACCCATAACTCTG+AGG - chr2_4:2335994-2336013 None:intergenic 45.0%
CTCAGAGTTATGGGTGTACA+AGG + chr2_4:2335992-2336011 Msa0308200:CDS 45.0%
CCTGCAAATGGCTCTTAACA+TGG - chr2_4:2336030-2336049 None:intergenic 45.0%
CCATGTTAAGAGCCATTTGC+AGG + chr2_4:2336027-2336046 Msa0308200:CDS 45.0%
GAGTGCACATTACCTGCAAA+TGG - chr2_4:2336042-2336061 None:intergenic 45.0%
TTGCAGGTAATGTGCACTCT+AGG + chr2_4:2336043-2336062 Msa0308200:intron 45.0%
CACTCTAGGTCCTCTTAATG+TGG + chr2_4:2336057-2336076 Msa0308200:intron 45.0%
GACTGACAAGAAAGAAACGG+GGG + chr2_4:2336327-2336346 Msa0308200:CDS 45.0%
ATTCAATGCCTCAAACCCGT+TGG + chr2_4:2336569-2336588 Msa0308200:intron 45.0%
!! TTTTTCCACCAACGGGTTTG+AGG - chr2_4:2336580-2336599 None:intergenic 45.0%
GCGACTACATGAACAATTGG+AGG + chr2_4:2336739-2336758 Msa0308200:CDS 45.0%
CTTATTGTCGAGCTCCTGTT+GGG - chr2_4:2337172-2337191 None:intergenic 45.0%
TCTTATTGTCGAGCTCCTGT+TGG - chr2_4:2337173-2337192 None:intergenic 45.0%
GCTCGACAATAAGACTGAAC+CGG + chr2_4:2337179-2337198 Msa0308200:CDS 45.0%
!! TAGCAGCTTTTTCTGGGTCA+GGG - chr2_4:2337209-2337228 None:intergenic 45.0%
!! TTAGCAGCTTTTTCTGGGTC+AGG - chr2_4:2337210-2337229 None:intergenic 45.0%
TCTGCTGAAAGACTCTTTGC+TGG - chr2_4:2337243-2337262 None:intergenic 45.0%
!! AATCTGGTGTCATCGGTTCA+TGG - chr2_4:2337281-2337300 None:intergenic 45.0%
!! CAACCAGAATCTGGTGTCAT+CGG - chr2_4:2337288-2337307 None:intergenic 45.0%
TTAGTTGATCGCTCGCCATT+AGG - chr2_4:2337336-2337355 None:intergenic 45.0%
CTTGACTCCAGTATCTGATG+AGG - chr2_4:2337411-2337430 None:intergenic 45.0%
ATCTCGGGTAAGGAAAACAG+CGG - chr2_4:2337457-2337476 None:intergenic 45.0%
GATCAAAGTGATCTCGGGTA+AGG - chr2_4:2337467-2337486 None:intergenic 45.0%
CATGTCTACAAGAAGTGGTG+AGG + chr2_4:2337497-2337516 Msa0308200:CDS 45.0%
GGTGAGGAATTGGATAAGCT+TGG + chr2_4:2337513-2337532 Msa0308200:CDS 45.0%
GAGGAATTGGATAAGCTTGG+TGG + chr2_4:2337516-2337535 Msa0308200:CDS 45.0%
! AATTGGATAAGCTTGGTGGC+AGG + chr2_4:2337520-2337539 Msa0308200:CDS 45.0%
!! TTGAGTTTTTGGGCCCATCT+TGG + chr2_4:2337764-2337783 Msa0308200:three_prime_UTR 45.0%
!! TGAGTTTTTGGGCCCATCTT+GGG + chr2_4:2337765-2337784 Msa0308200:three_prime_UTR 45.0%
! AGCTTTTGTTGATGTGGGGG+AGG + chr2_4:2334047-2334066 Msa0308200:intron 50.0%
! TTTAGGGTTTGGGTTTGGGG+GGG + chr2_4:2334431-2334450 Msa0308200:intron 50.0%
TTGGGTTCAAACCCGGACAA+AGG - chr2_4:2334785-2334804 None:intergenic 50.0%
GAAGGTCTTGGGTTCAAACC+CGG - chr2_4:2334792-2334811 None:intergenic 50.0%
ATGGGTGGGGGTAGATCAAT+TGG - chr2_4:2334825-2334844 None:intergenic 50.0%
! TTAGACCCATGCTTTTGCCC+GGG + chr2_4:2335120-2335139 Msa0308200:intron 50.0%
CATGGTCAGCACATAGAGTC+TGG + chr2_4:2335624-2335643 Msa0308200:CDS 50.0%
GTCAGCACATAGAGTCTGGT+AGG + chr2_4:2335628-2335647 Msa0308200:CDS 50.0%
AGACTGTTTCCTCCACTACC+AGG - chr2_4:2335666-2335685 None:intergenic 50.0%
GTTGATGCTGTGGCTCAACT+TGG + chr2_4:2335753-2335772 Msa0308200:CDS 50.0%
GGGGAAAATACTCACGATCG+GGG - chr2_4:2335782-2335801 None:intergenic 50.0%
TTACCTGACTGCTCATCCGA+TGG + chr2_4:2336156-2336175 Msa0308200:CDS 50.0%
AAGAGCAACAGCGACTCAGT+GGG + chr2_4:2337091-2337110 Msa0308200:CDS 50.0%
TGGGACATATCTCCTGGAGT+TGG - chr2_4:2337153-2337172 None:intergenic 50.0%
TCCAGGAGATATGTCCCAAC+AGG + chr2_4:2337155-2337174 Msa0308200:CDS 50.0%
TCCTGTTGGGACATATCTCC+TGG - chr2_4:2337159-2337178 None:intergenic 50.0%
!! AGCAGCTTTTTCTGGGTCAG+GGG - chr2_4:2337208-2337227 None:intergenic 50.0%
GAACCGATGACACCAGATTC+TGG + chr2_4:2337282-2337301 Msa0308200:CDS 50.0%
! CCAGATTCTGGTTGCCAAGT+CGG + chr2_4:2337294-2337313 Msa0308200:CDS 50.0%
TCTCCTGCTGACAGTCCTAA+TGG + chr2_4:2337318-2337337 Msa0308200:CDS 50.0%
GGTGCTTCCTCATCAGATAC+TGG + chr2_4:2337401-2337420 Msa0308200:CDS 50.0%
! TGATGTGGGGGAGGTTTTGC+TGG + chr2_4:2334056-2334075 Msa0308200:intron 55.0%
! GATGTGGGGGAGGTTTTGCT+GGG + chr2_4:2334057-2334076 Msa0308200:intron 55.0%
! TTAGGGTTTGGGTTTGGGGG+GGG + chr2_4:2334432-2334451 Msa0308200:intron 55.0%
TATGGGTGGATGGATGGGTG+GGG - chr2_4:2334838-2334857 None:intergenic 55.0%
GTATGGGTGGATGGATGGGT+GGG - chr2_4:2334839-2334858 None:intergenic 55.0%
TGTATGGGTGGATGGATGGG+TGG - chr2_4:2334840-2334859 None:intergenic 55.0%
! GTGAACCCGGGCAAAAGCAT+GGG - chr2_4:2335128-2335147 None:intergenic 55.0%
AGTGAACCCGGGCAAAAGCA+TGG - chr2_4:2335129-2335148 None:intergenic 55.0%
GAGGGGTGACTCAAACATGC+AGG - chr2_4:2335453-2335472 None:intergenic 55.0%
!! AGAGTCTGGTAGGAGCGCTA+TGG + chr2_4:2335638-2335657 Msa0308200:CDS 55.0%
AGCGCTATGGATCCTGGTAG+TGG + chr2_4:2335651-2335670 Msa0308200:CDS 55.0%
!! GCTATGGATCCTGGTAGTGG+AGG + chr2_4:2335654-2335673 Msa0308200:CDS 55.0%
GATGCTGTGGCTCAACTTGG+TGG + chr2_4:2335756-2335775 Msa0308200:CDS 55.0%
GGGTGTGGCACCTAAGGAAA+AGG - chr2_4:2335963-2335982 None:intergenic 55.0%
! TCCTTAGGTGCCACACCCAA+AGG + chr2_4:2335965-2335984 Msa0308200:intron 55.0%
ACCTTTGGGTGTGGCACCTA+AGG - chr2_4:2335969-2335988 None:intergenic 55.0%
TCTGAGGACACCTTTGGGTG+TGG - chr2_4:2335978-2335997 None:intergenic 55.0%
GTACCATCGGATGAGCAGTC+AGG - chr2_4:2336162-2336181 None:intergenic 55.0%
CAATGCCTCAAACCCGTTGG+TGG + chr2_4:2336572-2336591 Msa0308200:intron 55.0%
GAAGAGCAACAGCGACTCAG+TGG + chr2_4:2337090-2337109 Msa0308200:CDS 55.0%
AGAGCAACAGCGACTCAGTG+GGG + chr2_4:2337092-2337111 Msa0308200:CDS 55.0%
!! GGGGTTCTTTCAGAAGCACC+CGG + chr2_4:2337111-2337130 Msa0308200:CDS 55.0%
!! TGGTGCTGATACACCAGAGC+CGG - chr2_4:2337133-2337152 None:intergenic 55.0%
GGTGTATCAGCACCAACTCC+AGG + chr2_4:2337138-2337157 Msa0308200:CDS 55.0%
! TTTTCTGGGTCAGGGGTTGC+CGG - chr2_4:2337201-2337220 None:intergenic 55.0%
!! CCGACTTGGCAACCAGAATC+TGG - chr2_4:2337297-2337316 None:intergenic 55.0%
TGTCAGCAGGAGAACCGACT+TGG - chr2_4:2337311-2337330 None:intergenic 55.0%
TCGCCATTAGGACTGTCAGC+AGG - chr2_4:2337324-2337343 None:intergenic 55.0%
! GCGGTTGGTTGCTGGTATGA+TGG - chr2_4:2337438-2337457 None:intergenic 55.0%
! GGAAAACAGCGGTTGGTTGC+TGG - chr2_4:2337446-2337465 None:intergenic 55.0%
CGGGTAAGGAAAACAGCGGT+TGG - chr2_4:2337453-2337472 None:intergenic 55.0%
! TAGGGTTTGGGTTTGGGGGG+GGG + chr2_4:2334433-2334452 Msa0308200:intron 60.0%
ATGGGTGGATGGATGGGTGG+GGG - chr2_4:2334837-2334856 None:intergenic 60.0%
!! GGTAGGAGCGCTATGGATCC+TGG + chr2_4:2335645-2335664 Msa0308200:CDS 60.0%
!! CTTTCAGAAGCACCCGGCTC+TGG + chr2_4:2337117-2337136 Msa0308200:CDS 60.0%
!! GGTGCTGATACACCAGAGCC+GGG - chr2_4:2337132-2337151 None:intergenic 60.0%
! AGGGTTTGGGTTTGGGGGGG+GGG + chr2_4:2334434-2334453 Msa0308200:intron 65.0%
! GGGTTTGGGTTTGGGGGGGG+GGG + chr2_4:2334435-2334454 Msa0308200:intron 70.0%
! GGTTTGGGTTTGGGGGGGGG+GGG + chr2_4:2334436-2334455 Msa0308200:intron 70.0%
TTTGGGGGGGGGGGGACGAA+AGG + chr2_4:2334444-2334463 Msa0308200:intron 70.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 2333588 2337793 2333588 ID=Msa0308200;Name=Msa0308200
chr2_4 mRNA 2333588 2337793 2333588 ID=Msa0308200-mRNA-1;Parent=Msa0308200;Name=Msa0308200-mRNA-1;_AED=0.06;_eAED=0.06;_QI=151|1|1|1|0.83|0.71|7|242|330
chr2_4 exon 2333588 2333727 2333588 ID=Msa0308200-mRNA-1:exon:546;Parent=Msa0308200-mRNA-1
chr2_4 exon 2335556 2335786 2335556 ID=Msa0308200-mRNA-1:exon:547;Parent=Msa0308200-mRNA-1
chr2_4 exon 2335972 2336048 2335972 ID=Msa0308200-mRNA-1:exon:548;Parent=Msa0308200-mRNA-1
chr2_4 exon 2336135 2336177 2336135 ID=Msa0308200-mRNA-1:exon:549;Parent=Msa0308200-mRNA-1
chr2_4 exon 2336317 2336377 2336317 ID=Msa0308200-mRNA-1:exon:550;Parent=Msa0308200-mRNA-1
chr2_4 exon 2336691 2336760 2336691 ID=Msa0308200-mRNA-1:exon:551;Parent=Msa0308200-mRNA-1
chr2_4 exon 2337030 2337793 2337030 ID=Msa0308200-mRNA-1:exon:552;Parent=Msa0308200-mRNA-1
chr2_4 five_prime_UTR 2333588 2333727 2333588 ID=Msa0308200-mRNA-1:five_prime_utr;Parent=Msa0308200-mRNA-1
chr2_4 five_prime_UTR 2335556 2335566 2335556 ID=Msa0308200-mRNA-1:five_prime_utr;Parent=Msa0308200-mRNA-1
chr2_4 CDS 2335567 2335786 2335567 ID=Msa0308200-mRNA-1:cds;Parent=Msa0308200-mRNA-1
chr2_4 CDS 2335972 2336048 2335972 ID=Msa0308200-mRNA-1:cds;Parent=Msa0308200-mRNA-1
chr2_4 CDS 2336135 2336177 2336135 ID=Msa0308200-mRNA-1:cds;Parent=Msa0308200-mRNA-1
chr2_4 CDS 2336317 2336377 2336317 ID=Msa0308200-mRNA-1:cds;Parent=Msa0308200-mRNA-1
chr2_4 CDS 2336691 2336760 2336691 ID=Msa0308200-mRNA-1:cds;Parent=Msa0308200-mRNA-1
chr2_4 CDS 2337030 2337551 2337030 ID=Msa0308200-mRNA-1:cds;Parent=Msa0308200-mRNA-1
chr2_4 three_prime_UTR 2337552 2337793 2337552 ID=Msa0308200-mRNA-1:three_prime_utr;Parent=Msa0308200-mRNA-1
Gene Sequence

>Msa0308200

ATGTATCAGCCAACTAATGTTCCTGATTCGAGTTTAATTGGAAGCAATCCATTAGTTCATGGTCAGCACATAGAGTCTGGTAGGAGCGCTATGGATCCTGGTAGTGGAGGAAACAGTCTTGCCAACAACTCTAATCTCAACTCAAAGCAACGTTTGCGTTGGACACATGAGCTACATGAACGCTTTGTTGATGCTGTGGCTCAACTTGGTGGCCCCGATCGTGCCACACCCAAAGGTGTCCTCAGAGTTATGGGTGTACAAGGCTTAACAATTTACCATGTTAAGAGCCATTTGCAGAAATACCGACTAGCAAAATACTTACCTGACTGCTCATCCGATGAGGGGAAAAAGACTGACAAGAAAGAAACGGGGGATATGCTTTCCAATCTAGATGGTTCATCGGGGATGCAGATTACCGAAGCACTAAAGCTTCAAATGGAGGTACAGAAGCGACTACATGAACAATTGGAGGTGCAGAGACAGCTACAATTAAGGATAGAAGCTCAGGGAAAATATTTGAAAAAGATAATCGAAGAGCAACAGCGACTCAGTGGGGTTCTTTCAGAAGCACCCGGCTCTGGTGTATCAGCACCAACTCCAGGAGATATGTCCCAACAGGAGCTCGACAATAAGACTGAACCGGCAACCCCTGACCCAGAAAAAGCTGCTAAAGAGCATGCACCAGCAAAGAGTCTTTCAGCAGAATCTTTTTCATCTCACCATGAACCGATGACACCAGATTCTGGTTGCCAAGTCGGTTCTCCTGCTGACAGTCCTAATGGCGAGCGATCAACTAAAAAGCAACGAGTAAGCGTGGAAGAAGCGTATTTGAAATCAGACATGGTGCTTCCTCATCAGATACTGGAGTCAAGCATGCCATCATACCAGCAACCAACCGCTGTTTTCCTTACCCGAGATCACTTTGATCCTTCTTTAGGCATGTCTACAAGAAGTGGTGAGGAATTGGATAAGCTTGGTGGCAGGAATATGTGA
Protein sequence

>Msa0308200

MYQPTNVPDSSLIGSNPLVHGQHIESGRSAMDPGSGGNSLANNSNLNSKQRLRWTHELHERFVDAVAQLGGPDRATPKGVLRVMGVQGLTIYHVKSHLQKYRLAKYLPDCSSDEGKKTDKKETGDMLSNLDGSSGMQITEALKLQMEVQKRLHEQLEVQRQLQLRIEAQGKYLKKIIEEQQRLSGVLSEAPGSGVSAPTPGDMSQQELDNKTEPATPDPEKAAKEHAPAKSLSAESFSSHHEPMTPDSGCQVGSPADSPNGERSTKKQRVSVEEAYLKSDMVLPHQILESSMPSYQQPTAVFLTRDHFDPSLGMSTRSGEELDKLGGRNM*