AlfalfaGEDB | Msa0280650

M. sativa cultivar zhongmu-4 / Msa0280650

KEGG: K15032
GO: GO:0001101 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006970 GO:0006996 GO:0008150 GO:0008152 GO:0008380 GO:0009507 GO:0009536 GO:0009628 GO:0009651 GO:0009657 GO:0009658 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0010467 GO:0016043 GO:0016070 GO:0019843 GO:0022607 GO:0022613 GO:0022618 GO:0032502 GO:0033993 GO:0034622 GO:0034641 GO:0042221 GO:0042254 GO:0042255 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0050896 GO:0065003 GO:0070925 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0097159 GO:0097305 GO:1901360 GO:1901363 GO:1901700
PFAM: PF02536.17

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0280650	AES65036.2	96.748	492	16	0	1	492	66	557	0.0	976

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0280650	sp\|F4JVI3\|MTEF5_ARATH	61.915	470	167	5	20	479	1	468	0.0	561

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0280650	G7IMU1	96.748	492	16	0	1	492	66	557	0.0	976

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TFs/TRs:

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Gene ID	Type	Classification
Msa0280650	TR	mTERF

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0085190	Msa0280650	0.807550	4.786465e-50	-8.615850e-47
Msa0280650	Msa1110350	0.800067	1.714737e-48	-8.615850e-47
Msa0280650	Msa1154020	0.803600	3.226679e-49	-8.615850e-47
Msa0280650	Msa1409530	0.806684	7.297935e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0280650	MtrunA17_Chr2g0294671	95.968	496	16	1	1	492	1	496	0.0	970
Msa0280650	MtrunA17_Chr6g0448821	30.078	256	174	3	227	478	102	356	5.42e-31	123
Msa0280650	MtrunA17_Chr5g0417641	35.096	208	129	5	277	479	87	293	1.22e-29	118
Msa0280650	MtrunA17_Chr5g0428941	29.455	275	184	6	213	480	213	484	2.62e-29	120
Msa0280650	MtrunA17_Chr5g0428941	23.050	282	168	6	207	484	139	375	4.66e-13	71.2
Msa0280650	MtrunA17_Chr1g0212691	29.474	285	189	6	207	483	175	455	9.59e-29	119
Msa0280650	MtrunA17_Chr1g0212691	23.485	264	176	5	185	426	67	326	1.66e-13	72.8
Msa0280650	MtrunA17_Chr4g0034981	24.913	289	208	7	207	488	309	595	1.79e-26	113
Msa0280650	MtrunA17_Chr4g0034981	25.000	256	179	5	240	486	271	522	4.57e-20	93.6
Msa0280650	MtrunA17_Chr4g0034981	29.949	197	135	3	204	398	378	573	1.12e-14	76.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0280650	AT4G14605.2	61.915	470	167	5	20	479	1	468	0.0	561
Msa0280650	AT4G14605.1	61.915	470	167	5	20	479	1	468	0.0	561
Msa0280650	AT2G21710.1	28.912	294	194	7	207	490	316	604	4.96e-33	133
Msa0280650	AT2G21710.1	30.366	191	130	3	204	392	385	574	2.74e-13	72.8
Msa0280650	AT2G21710.1	28.114	281	182	7	73	352	306	567	6.53e-13	71.6
Msa0280650	AT2G44020.1	27.726	321	186	6	206	483	143	460	1.40e-29	122
Msa0280650	AT4G38160.4	27.372	274	186	5	217	480	52	322	3.79e-27	112
Msa0280650	AT4G38160.4	30.719	153	103	3	203	352	110	262	4.72e-14	73.6
Msa0280650	AT4G38160.1	27.372	274	186	5	217	480	52	322	3.79e-27	112
Msa0280650	AT4G38160.1	30.719	153	103	3	203	352	110	262	4.72e-14	73.6

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Find 120 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAAGGTTAATACAGCCT+TGG	0.517039	2_3:-15506427	None:intergenic
AAACTATAGTAGGCAAAAGC+TGG	0.426748	2_3:-15506964	None:intergenic
AAAGAAAATGCAAGTTGATT+AGG	0.286687	2_3:+15507728	Msa0280650:CDS
AAAGCCGTGTCTCGAGTGAG+TGG	0.646198	2_3:+15506844	Msa0280650:CDS
AAATAAATAGCATCAAAACC+TGG	0.592850	2_3:-15508128	None:intergenic
AACCCTATTGGCAGCAGAAA+AGG	0.405382	2_3:+15506398	Msa0280650:CDS
AACTATAGTAGGCAAAAGCT+GGG	0.542556	2_3:-15506963	None:intergenic
AAGAAGACCCTTGTAGAGGA+AGG	0.547271	2_3:-15506210	None:intergenic
AAGTGATTAGAGGATGCTTT+TGG	0.393419	2_3:+15508095	Msa0280650:three_prime_UTR
AAGTTAGCAGGGCTGGGAAA+CGG	0.513243	2_3:-15507173	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATGGTACAATAAATAGAGAG+AGG	+	chr2_3:15505222-15505241	Msa0280650:five_prime_UTR	30.0%
TGGTACAATAAATAGAGAGA+GGG	+	chr2_3:15505223-15505242	Msa0280650:five_prime_UTR	30.0%
AAGAAAGAGATTGTACCTTA+TGG	-	chr2_3:15505611-15505630	None:intergenic	30.0%
CTTATCTTCATAAGCTATCT+AGG	-	chr2_3:15505936-15505955	None:intergenic	30.0%
ATTCGTTTGAGCTTATCAAT+TGG	-	chr2_3:15506031-15506050	None:intergenic	30.0%
TTCACTCTAAACACAAAACT+TGG	+	chr2_3:15506535-15506554	Msa0280650:CDS	30.0%
GTCTCATTGACATAATAATC+AGG	+	chr2_3:15506637-15506656	Msa0280650:intron	30.0%
GAATGAATATTGACCAGATT+AGG	+	chr2_3:15506923-15506942	Msa0280650:CDS	30.0%
TTCTCATTATCCTCACTAAA+AGG	+	chr2_3:15507049-15507068	Msa0280650:CDS	30.0%
GATTCAAAGAATTTCATGGT+GGG	-	chr2_3:15507114-15507133	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_3	gene	15505221	15508170	15505221	ID=Msa0280650;Name=Msa0280650
chr2_3	mRNA	15505221	15508170	15505221	ID=Msa0280650-mRNA-1;Parent=Msa0280650;Name=Msa0280650-mRNA-1;_AED=0.05;_eAED=0.05;_QI=263\|1\|1\|1\|1\|1\|4\|421\|492
chr2_3	exon	15505221	15505614	15505221	ID=Msa0280650-mRNA-1:exon:6463;Parent=Msa0280650-mRNA-1
chr2_3	exon	15506200	15506249	15506200	ID=Msa0280650-mRNA-1:exon:6464;Parent=Msa0280650-mRNA-1
chr2_3	exon	15506361	15506567	15506361	ID=Msa0280650-mRNA-1:exon:6465;Parent=Msa0280650-mRNA-1
chr2_3	exon	15506659	15508170	15506659	ID=Msa0280650-mRNA-1:exon:6466;Parent=Msa0280650-mRNA-1
chr2_3	five_prime_UTR	15505221	15505483	15505221	ID=Msa0280650-mRNA-1:five_prime_utr;Parent=Msa0280650-mRNA-1
chr2_3	CDS	15505484	15505614	15505484	ID=Msa0280650-mRNA-1:cds;Parent=Msa0280650-mRNA-1
chr2_3	CDS	15506200	15506249	15506200	ID=Msa0280650-mRNA-1:cds;Parent=Msa0280650-mRNA-1
chr2_3	CDS	15506361	15506567	15506361	ID=Msa0280650-mRNA-1:cds;Parent=Msa0280650-mRNA-1
chr2_3	CDS	15506659	15507749	15506659	ID=Msa0280650-mRNA-1:cds;Parent=Msa0280650-mRNA-1
chr2_3	three_prime_UTR	15507750	15508170	15507750	ID=Msa0280650-mRNA-1:three_prime_utr;Parent=Msa0280650-mRNA-1

Gene Sequence

>Msa0280650

ATGTTTGTTGTTGCATCTTCCATTTGCAGTGATATTCCACTCTTAAAACCAATAAAGATGAAAACTTTCTCCTCAATTCAACCACCCCACAATTTCCATTCCGTGCCATTTTTCTCTTACACTTCCATAAGGACTCAACTTCCTTCCTCTACAAGGGTCTTCTTTTCTCAGGAAAAATCTGACACAGATGAGTCATTAAACGTAAAACTGTTGCCTCCAACCCTATTGGCAGCAGAAAAGGAAGAAGCCAAGGCTGTATTAACCTTGTTTTTGAAGAAACGAGGTTTGAGCAATGCAAATGCAGCAAGAACGATTAACAAGTCAGATCCTTTTATTGATCACCTTCTCTCGAAGCTTCACTCTAAACACAAAACTTGGTACCTTTCAGGGAGAGAGCTTACAACTCTTGAGATCAGGGATGCTCTTATCTCTTATCTCGAATCACTTTACGAAGAGCATGGAGAAATTCTAGTGGATGTAGTGGAAAACTATCCAAATCCACCTGTTAAGGATAAATCGGACGCACCAATTCCACCTCCTAGTCCTAGTCCAGCGGTAGACTCTAAGAAAGTTAAAGCCGTGTCTCGAGTGAGTGGTATAGACCCTGCTGAGGGAAATCTTCGTCCTCATATTGCTTATCTGATGGAACTCGGAATGAATATTGACCAGATTAGGACTATTATGCGACGATTCCCAGCTTTTGCCTACTATAGTTTGGAGGGTAAAATTAAGCCAGTGGTTGAGTTTTTTCTTGAACTTGGAGTACCAAAAGAAAAAATTCTCATTATCCTCACTAAAAGGCCTCAATTATGTGGAATCAGTCTATCTAAAAATCTTAAGCCCACCATGAAATTCTTTGAATCTTTAGGTGTTGACAAGGAACAATGGGCGAAAGTGATCTACCGTTTCCCAGCCCTGCTAACTTATAGCACGCAGAAGATTAATGAAAGTGTAGATTTTCTCCGTGAATTTGGTGTCTCAGAAGAGAACATAGGTAAGATTTTAACTCGCTGCCCAACTATTGTTAGTTACAGTGTAGAGGACAATCTTCGACCGACAGCAATGTACTTCCGTTCTCTAGGGGTTGATGTTGGCCTTCTTCTATTCAATTGTCCACAAAATTTTGGTCTTAGCATTGAGGCCAATATAAAGCCCGTAACACAATTTTTCTTGGAGAGAGGATACACTGTGGAAGAAATTGGGATTATGATTATGAGATATGGAATGTTGTACACATTCAGCTTGACTGAGAATTTGATGCCGAAATGGGATTACTTTTTGACTATGGATTACCCAAAATCTGAGTTGGTTAAATTCCCTCAATTTTTTGGATACAGTTTAGAGCAAAGAATTAAACCGAGATATGCACGCGTGAAAAATTCTGGGGTGAGATTACTGTTGAATCAGGTTCTTTCACTGTCAAGCAGCAACTTTGACGAGATCTTAAGAAAGAAAATAAAGAAAATGCAAGTTGATTAG

Protein sequence

>Msa0280650

MFVVASSICSDIPLLKPIKMKTFSSIQPPHNFHSVPFFSYTSIRTQLPSSTRVFFSQEKSDTDESLNVKLLPPTLLAAEKEEAKAVLTLFLKKRGLSNANAARTINKSDPFIDHLLSKLHSKHKTWYLSGRELTTLEIRDALISYLESLYEEHGEILVDVVENYPNPPVKDKSDAPIPPPSPSPAVDSKKVKAVSRVSGIDPAEGNLRPHIAYLMELGMNIDQIRTIMRRFPAFAYYSLEGKIKPVVEFFLELGVPKEKILIILTKRPQLCGISLSKNLKPTMKFFESLGVDKEQWAKVIYRFPALLTYSTQKINESVDFLREFGVSEENIGKILTRCPTIVSYSVEDNLRPTAMYFRSLGVDVGLLLFNCPQNFGLSIEANIKPVTQFFLERGYTVEEIGIMIMRYGMLYTFSLTENLMPKWDYFLTMDYPKSELVKFPQFFGYSLEQRIKPRYARVKNSGVRLLLNQVLSLSSSNFDEILRKKIKKMQVD*