AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0286170

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0286170 MtrunA17_Chr7g0263231 31.845 672 418 16 46 690 411 1069 1.83e-108 354
Msa0286170 MtrunA17_Chr2g0308821 31.476 664 415 13 59 690 4 659 3.58e-108 343
Msa0286170 MtrunA17_Chr7g0263261 32.053 677 410 18 46 690 352 1010 2.97e-104 342
Msa0286170 MtrunA17_Chr8g0359341 30.396 681 424 17 58 705 59 722 1.91e-102 330
Msa0286170 MtrunA17_Chr8g0354631 31.647 692 421 20 33 690 30 703 2.33e-100 325
Msa0286170 MtrunA17_Chr5g0402891 33.448 580 352 13 134 690 11 579 6.92e-97 312
Msa0286170 MtrunA17_Chr2g0333261 29.868 683 431 17 39 689 12 678 2.13e-96 313
Msa0286170 MtrunA17_Chr8g0383211 28.656 677 401 12 50 689 4 635 5.06e-95 308
Msa0286170 MtrunA17_Chr4g0003151 28.000 675 443 14 55 700 13 673 7.03e-92 300
Msa0286170 MtrunA17_Chr4g0052681 32.472 542 344 11 160 689 27 558 8.39e-86 282
Msa0286170 MtrunA17_Chr8g0383041 32.515 489 310 10 210 689 78 555 5.07e-82 272
Msa0286170 MtrunA17_Chr8g0383081 31.073 531 348 10 166 689 31 550 2.30e-80 267
Msa0286170 MtrunA17_Chr8g0383071 39.502 281 162 4 225 501 34 310 6.72e-64 214
Msa0286170 MtrunA17_Chr4g0052601 32.569 436 260 14 45 468 11 424 2.08e-62 214
Msa0286170 MtrunA17_Chr4g0062411 25.421 653 435 16 65 707 55 665 4.78e-48 180
Msa0286170 MtrunA17_Chr8g0383051 32.740 281 179 5 166 441 46 321 1.19e-40 151
Msa0286170 MtrunA17_Chr4g0053391 34.211 266 149 7 437 695 1 247 1.99e-38 144
Msa0286170 MtrunA17_Chr5g0408131 35.961 203 123 3 487 689 23 218 1.10e-32 126
Msa0286170 MtrunA17_Chr4g0052611 36.449 214 113 5 484 691 9 205 6.29e-32 124
Msa0286170 MtrunA17_Chr1g0192041 33.735 166 105 2 390 553 62 224 9.52e-30 118
Msa0286170 MtrunA17_Chr8g0383181 27.740 292 169 8 67 343 22 286 2.47e-26 110
Msa0286170 MtrunA17_Chr8g0383061 30.435 207 130 6 487 689 1 197 4.42e-19 87.0
Msa0286170 MtrunA17_Chr8g0390371 46.575 73 36 1 623 695 11 80 1.59e-14 69.7
Msa0286170 MtrunA17_Chr3g0108001 45.205 73 37 1 623 695 7 76 2.98e-14 69.3
Msa0286170 MtrunA17_Chr8g0383171 27.374 179 117 5 514 689 63 231 1.73e-13 71.6
Msa0286170 MtrunA17_Chr1g0162951 46.667 60 29 1 642 701 1 57 3.01e-12 62.0
Msa0286170 MtrunA17_Chr5g0440991 29.032 124 79 3 401 515 1 124 3.02e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0286170 AT3G42170.2 29.717 636 410 14 67 689 41 652 1.30e-97 316
Msa0286170 AT3G42170.1 29.384 633 416 13 67 689 63 674 1.54e-97 316
Msa0286170 AT1G18560.1 24.625 666 447 16 57 707 40 665 1.20e-41 162

Find 123 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATAAATTATATAAAGTTAA+AGG 0.209633 2_3:+26727992 None:intergenic
TCTGTTACAAATATAATTTC+TGG 0.238190 2_3:-26729619 Msa0286170:CDS
TTTGCTTGACCACGGATTTA+TGG 0.240127 2_3:-26730272 Msa0286170:CDS
GAAATAAAACTTCTCCAGTT+TGG 0.240607 2_3:-26730752 Msa0286170:CDS
TCTTCCTCAACAATACTAAA+TGG 0.270419 2_3:+26730432 None:intergenic
GGACAAGCAAGTTTAGTATA+TGG 0.273052 2_3:+26729950 None:intergenic
TCATCTCCGGGGTAAATTAA+AGG 0.288608 2_3:+26729379 None:intergenic
CTTCAAAGAAGTGAAGTTAA+AGG 0.299486 2_3:-26730724 Msa0286170:CDS
TATGTCCCTTGCTTCTGTTT+TGG 0.319533 2_3:-26729440 Msa0286170:CDS
ATGATTGATGAGAAGCTTTC+CGG 0.328359 2_3:-26730540 Msa0286170:CDS
GACCACCCTGTGTCTCTTGT+TGG 0.332360 2_3:+26729223 None:intergenic
AGAACTAGTGCTGATCAAAA+TGG 0.335518 2_3:-26729259 Msa0286170:CDS
AGGGGAGAGCAATTGGAGTT+TGG 0.335928 2_3:-26728112 Msa0286170:three_prime_UTR
CCATTTCATTGATGCTGATT+GGG 0.352176 2_3:-26730208 Msa0286170:CDS
AAATAAAACTTCTCCAGTTT+GGG 0.353519 2_3:-26730751 Msa0286170:CDS
GCCATTTCATTGATGCTGAT+TGG 0.359357 2_3:-26730209 Msa0286170:CDS
TCTGAAGCCACAGTAGATAT+TGG 0.367386 2_3:+26729007 None:intergenic
AGCTAAAAGTTCTACTTTCA+AGG 0.369693 2_3:-26730023 Msa0286170:CDS
CTCTTCCAGATGAAGATGAT+TGG 0.371453 2_3:-26729684 Msa0286170:CDS
AAGCCCTCTTCTCCATCATT+TGG 0.374987 2_3:+26730912 None:intergenic
AGAGTATGCTGATATGCTTC+AGG 0.386838 2_3:-26729305 Msa0286170:CDS
CTTTAATTTGTGGTGGTGAT+TGG 0.392374 2_3:-26728913 Msa0286170:CDS
ATAGAATACATGGTGCTAAC+TGG 0.399590 2_3:-26730231 Msa0286170:CDS
CTTTAAATGTCTCAAAGAAT+GGG 0.401248 2_3:-26730112 Msa0286170:CDS
TTGCTTGTCCAAGATGGTAT+TGG 0.401789 2_3:-26729937 Msa0286170:CDS
TCTTTAAATGTCTCAAAGAA+TGG 0.404654 2_3:-26730113 Msa0286170:CDS
GTCTTGAAGGGGAGAGCAAT+TGG 0.409399 2_3:-26728119 Msa0286170:three_prime_UTR
ATGAGTAAAGATTAAGTTGA+AGG 0.409636 2_3:-26728171 Msa0286170:three_prime_UTR
TTATTCTTCCAATACCATCT+TGG 0.410788 2_3:+26729929 None:intergenic
CCCAATCAGCATCAATGAAA+TGG 0.414858 2_3:+26730208 None:intergenic
TCTTTGAAGTGATCCCAAAC+TGG 0.416027 2_3:+26730738 None:intergenic
TAGTACGTCAATTGAATCAT+TGG 0.416517 2_3:-26730946 Msa0286170:CDS
CCCTTGAATGTCTCTTCAAA+TGG 0.418648 2_3:+26730631 None:intergenic
AGCAAGATTACGAACATTCT+CGG 0.424413 2_3:-26729854 Msa0286170:CDS
ACGAGAAGCCACTGCACATT+GGG 0.435944 2_3:-26730472 Msa0286170:CDS
TTTAGTATTGTTGAGGAAGA+AGG 0.438752 2_3:-26730429 Msa0286170:CDS
GACAAGCAAGTTTAGTATAT+GGG 0.441530 2_3:+26729951 None:intergenic
TTCTTGATTGCCCTACTCGC+TGG 0.443318 2_3:-26729792 Msa0286170:CDS
CCACCATTCCAAGGCACAAA+AGG 0.447229 2_3:+26729090 None:intergenic
TCATGATCATATTTAGCACT+TGG 0.449353 2_3:+26730501 None:intergenic
GACATATGACCATTATCGTT+AGG 0.449440 2_3:+26729112 None:intergenic
ACTTACAGCCCTAACGATAA+TGG 0.449866 2_3:-26729121 Msa0286170:CDS
TCTTCCAGATGAAGATGATT+GGG 0.455559 2_3:-26729683 Msa0286170:CDS
GGACCCTAGGATCAAAATGA+TGG 0.456567 2_3:-26729419 Msa0286170:CDS
ACATTCAAGGGAAGTATGTC+AGG 0.460996 2_3:-26730619 Msa0286170:CDS
ATATAGAGTATTATCTCATA+TGG 0.462323 2_3:-26729050 Msa0286170:CDS
CAACGATGCTGTTATTCAAA+AGG 0.462531 2_3:-26730781 Msa0286170:CDS
AATTATTCTGTTGTTGCAAG+TGG 0.466913 2_3:-26730663 Msa0286170:CDS
GGTGTTGCTTCATTTGTTAG+TGG 0.469430 2_3:+26730840 None:intergenic
TCTGGAAGAGTTGTATAACT+TGG 0.473625 2_3:+26729697 None:intergenic
GCAAGATATAAATTAGAAGT+TGG 0.480417 2_3:+26729586 None:intergenic
GCAACACCGGAAAATGAAGT+TGG 0.483664 2_3:-26730825 Msa0286170:CDS
ACATGGAGTTCCAGCGAGTA+GGG 0.488258 2_3:+26729782 None:intergenic
GAACTAGTGCTGATCAAAAT+GGG 0.493299 2_3:-26729258 Msa0286170:CDS
TTATTCCCTTGATGTTAAGT+AGG 0.495461 2_3:-26728743 Msa0286170:intron
GCCATTTGAAGAGACATTCA+AGG 0.497363 2_3:-26730632 Msa0286170:CDS
AGACATGGCAAATGCGATGA+AGG 0.505997 2_3:-26729500 Msa0286170:CDS
ACCGTTGAAACTTTAATTTG+TGG 0.507663 2_3:-26728923 Msa0286170:CDS
CTTCAGGAGCATAGAGAAGA+AGG 0.509895 2_3:-26729289 Msa0286170:CDS
AACGTACCTACTTAACATCA+AGG 0.512693 2_3:+26728737 None:intergenic
ACATATGACCATTATCGTTA+GGG 0.512841 2_3:+26729113 None:intergenic
TGGATTCATAAGTAGTGGTC+CGG 0.516119 2_3:+26730521 None:intergenic
AGTACGTCAATTGAATCATT+GGG 0.521505 2_3:-26730945 Msa0286170:CDS
TGCTATTCCAATATCTACTG+TGG 0.523521 2_3:-26729014 Msa0286170:CDS
TTCTAATTTATATCTTGCTG+AGG 0.525402 2_3:-26729581 Msa0286170:CDS
CAACACCGGAAAATGAAGTT+GGG 0.527758 2_3:-26730824 Msa0286170:CDS
GGGAATAATGTGATTTCTGT+CGG 0.531032 2_3:+26728758 None:intergenic
AAATAAAATATTTAGTGTGT+CGG 0.531663 2_3:-26730082 Msa0286170:CDS
GTCTATTATATGCATGCTTG+TGG 0.533217 2_3:-26727970 None:intergenic
AACTGACAACATTTGATACA+TGG 0.534684 2_3:+26729765 None:intergenic
TAAAATATTTAGTGTGTCGG+TGG 0.536956 2_3:-26730079 Msa0286170:CDS
ACCACAAATTAAAGTTTCAA+CGG 0.539404 2_3:+26728922 None:intergenic
ATAGTTCAACAACTAAAGCT+TGG 0.540231 2_3:-26729829 Msa0286170:CDS
GCACTTGGATTCATAAGTAG+TGG 0.541124 2_3:+26730516 None:intergenic
AAAGATTAAGTTGAAGGCTG+TGG 0.541132 2_3:-26728165 Msa0286170:three_prime_UTR
GAAGCATATCAGCATACTCT+AGG 0.542630 2_3:+26729308 None:intergenic
GGAGGTTCATCACCAAATGA+TGG 0.548483 2_3:-26730924 Msa0286170:CDS
ATTGGCTTCGAGTATTGCAT+GGG 0.548627 2_3:-26728895 Msa0286170:CDS
GATTGGCTTCGAGTATTGCA+TGG 0.550233 2_3:-26728896 Msa0286170:CDS
TGCTTTCCTTTAATTTACCC+CGG 0.550404 2_3:-26729385 Msa0286170:CDS
TCGATCTTGAAAACGTGGAA+AGG 0.551102 2_3:+26729723 None:intergenic
TCAACACCACGTCGAGGAGG+AGG 0.558551 2_3:+26730150 None:intergenic
CTAAACTTGCTTGTCCAAGA+TGG 0.561967 2_3:-26729943 Msa0286170:CDS
ATTCAACTTCCACCATTCCA+AGG 0.562144 2_3:+26729081 None:intergenic
AAAGAAGTGAAGTTAAAGGA+TGG 0.565368 2_3:-26730720 Msa0286170:CDS
AACGAGAAGCCACTGCACAT+TGG 0.572418 2_3:-26730473 Msa0286170:CDS
TCACCAAATGATGGAGAAGA+GGG 0.573695 2_3:-26730915 Msa0286170:CDS
AAATAAGATTTGCTTGACCA+CGG 0.577624 2_3:-26730280 Msa0286170:CDS
TTATCGTTAGGGCTGTAAGT+TGG 0.583645 2_3:+26729124 None:intergenic
AAATCAAAAGATAGAATACA+TGG 0.583652 2_3:-26730241 Msa0286170:CDS
GTTCAACAACTAAAGCTTGG+TGG 0.587329 2_3:-26729826 Msa0286170:CDS
ATCACCAAATGATGGAGAAG+AGG 0.588933 2_3:-26730916 Msa0286170:CDS
TTTCATATGAGAAAATTAGT+CGG 0.590543 2_3:-26730377 Msa0286170:CDS
GACCCTAGGATCAAAATGAT+GGG 0.591637 2_3:-26729418 Msa0286170:CDS
ACATCCATTTAGTATTGTTG+AGG 0.596544 2_3:-26730436 Msa0286170:CDS
AGAAGTTAAAGTCAACTTTG+AGG 0.600517 2_3:-26730308 Msa0286170:CDS
TTGTGACTTCCATAAATCCG+TGG 0.602894 2_3:+26730263 None:intergenic
AGGGTCCAAAACAGAAGCAA+GGG 0.604905 2_3:+26729435 None:intergenic
CCATTTGAAGAGACATTCAA+GGG 0.606049 2_3:-26730631 Msa0286170:CDS
TAGAAGAATGTAGAGAAGCT+CGG 0.609825 2_3:+26728945 None:intergenic
AAAAGTTTGCAAGCTGCTAG+AGG 0.611700 2_3:-26729650 Msa0286170:CDS
GTTGAAGGCTGTGGCAAATG+TGG 0.614214 2_3:-26728156 Msa0286170:three_prime_UTR
TTGGAGCCAACAAGAGACAC+AGG 0.615353 2_3:-26729229 Msa0286170:CDS
TAGGGTCCAAAACAGAAGCA+AGG 0.615539 2_3:+26729434 None:intergenic
ACGTACCTACTTAACATCAA+GGG 0.621396 2_3:+26728738 None:intergenic
CATGTTCCTCCTCCTCGACG+TGG 0.623565 2_3:-26730156 Msa0286170:CDS
TTTAAATGTCTCAAAGAATG+GGG 0.629030 2_3:-26730111 Msa0286170:CDS
TGATCAAAATGGGCTGACAT+TGG 0.630883 2_3:-26729248 Msa0286170:CDS
CATCATAACCCAATGTGCAG+TGG 0.633413 2_3:+26730464 None:intergenic
TTAGTATATGGGCACAACAA+CGG 0.638288 2_3:+26729962 None:intergenic
GTTGAAACTTTAATTTGTGG+TGG 0.639553 2_3:-26728920 Msa0286170:CDS
GGTGTTTCAAATCAAGCAAG+TGG 0.641921 2_3:-26729560 Msa0286170:CDS
ACTAACAAATGAAGCAACAC+CGG 0.649014 2_3:-26730838 Msa0286170:CDS
ACGTCAATTGAATCATTGGG+AGG 0.653810 2_3:-26730942 Msa0286170:CDS
TACATGGAGTTCCAGCGAGT+AGG 0.658314 2_3:+26729781 None:intergenic
TTGCTGTCTATGAATCTGAA+AGG 0.661595 2_3:-26730332 Msa0286170:CDS
GGTTCTCGATCTTGAAAACG+TGG 0.664593 2_3:+26729718 None:intergenic
GTCTAACTTCATGAAAGACA+TGG 0.678953 2_3:-26729515 Msa0286170:CDS
ATATCAACACCACGTCGAGG+AGG 0.692782 2_3:+26730147 None:intergenic
GCAATATCAACACCACGTCG+AGG 0.705440 2_3:+26730144 None:intergenic
GAATGGTGGAAGTTGAATAG+CGG 0.711081 2_3:-26729076 Msa0286170:CDS
TGGAGCCAACAAGAGACACA+GGG 0.732152 2_3:-26729228 Msa0286170:CDS
AGCCAACAAGAGACACAGGG+TGG 0.786904 2_3:-26729225 Msa0286170:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATAAATTATATAAAGTTAA+AGG + chr2_3:26730938-26730957 None:intergenic 10.0%
!! AAATAAAATATTTAGTGTGT+CGG - chr2_3:26728845-26728864 Msa0286170:intron 15.0%
!! AAGAGAAAATATATTACTTT+AGG + chr2_3:26730070-26730089 None:intergenic 15.0%
!!! CAATATTTTTTATTAATAGG+TGG - chr2_3:26730138-26730157 Msa0286170:CDS 15.0%
!! ATCAATAAACAACTTAATAA+AGG + chr2_3:26730636-26730655 None:intergenic 15.0%
!! TTTCATATGAGAAAATTAGT+CGG - chr2_3:26728550-26728569 Msa0286170:intron 20.0%
!! AAATCAAAAGATAGAATACA+TGG - chr2_3:26728686-26728705 Msa0286170:intron 20.0%
!! TCTGTTACAAATATAATTTC+TGG - chr2_3:26729308-26729327 Msa0286170:CDS 20.0%
!! AGCTAGAAAAAATATAGAAA+AGG - chr2_3:26729571-26729590 Msa0286170:CDS 20.0%
!!! ATATAGAGTATTATCTCATA+TGG - chr2_3:26729877-26729896 Msa0286170:CDS 20.0%
!! ATAAGAAAAAAAGATGCATT+GGG - chr2_3:26730315-26730334 Msa0286170:CDS 20.0%
!!! TAAAGATAGCATTTTTGTAT+TGG - chr2_3:26730440-26730459 Msa0286170:CDS 20.0%
!!! AAAGATAGCATTTTTGTATT+GGG - chr2_3:26730441-26730460 Msa0286170:CDS 20.0%
! AAATAAAACTTCTCCAGTTT+GGG - chr2_3:26728176-26728195 Msa0286170:three_prime_UTR 25.0%
! TAAAGGATGGAACAAAAAAA+TGG - chr2_3:26728220-26728239 Msa0286170:three_prime_UTR 25.0%
! TCTTTAAATGTCTCAAAGAA+TGG - chr2_3:26728814-26728833 Msa0286170:intron 25.0%
! CTTTAAATGTCTCAAAGAAT+GGG - chr2_3:26728815-26728834 Msa0286170:intron 25.0%
! TTTAAATGTCTCAAAGAATG+GGG - chr2_3:26728816-26728835 Msa0286170:intron 25.0%
! GCAAGATATAAATTAGAAGT+TGG + chr2_3:26729344-26729363 None:intergenic 25.0%
! TTCTAATTTATATCTTGCTG+AGG - chr2_3:26729346-26729365 Msa0286170:CDS 25.0%
! AGGAAAAATTTGACAAGTAT+TGG - chr2_3:26729447-26729466 Msa0286170:CDS 25.0%
! ACCACAAATTAAAGTTTCAA+CGG + chr2_3:26730008-26730027 None:intergenic 25.0%
! GATAAGAAAAAAAGATGCAT+TGG - chr2_3:26730314-26730333 Msa0286170:CDS 25.0%
! CAACTATGATATGACATTTA+CGG - chr2_3:26730541-26730560 Msa0286170:CDS 25.0%
! AACTATGATATGACATTTAC+GGG - chr2_3:26730542-26730561 Msa0286170:CDS 25.0%
!! GCTAAAAGCATAACAAAAAA+AGG + chr2_3:26730599-26730618 None:intergenic 25.0%
!! ATTGATATGTAGCATTTTTC+AGG - chr2_3:26730650-26730669 Msa0286170:CDS 25.0%
!!! ATGAGTAAAGATTAAGTTGA+AGG - chr2_3:26730756-26730775 Msa0286170:CDS 25.0%
!! TGAATACAATTTTGTCTTGA+AGG - chr2_3:26730795-26730814 Msa0286170:CDS 25.0%
!! GAATACAATTTTGTCTTGAA+GGG - chr2_3:26730796-26730815 Msa0286170:CDS 25.0%
!! AATACAATTTTGTCTTGAAG+GGG - chr2_3:26730797-26730816 Msa0286170:CDS 25.0%
TAGTACGTCAATTGAATCAT+TGG - chr2_3:26727981-26728000 Msa0286170:three_prime_UTR 30.0%
AGTACGTCAATTGAATCATT+GGG - chr2_3:26727982-26728001 Msa0286170:three_prime_UTR 30.0%
GAAATAAAACTTCTCCAGTT+TGG - chr2_3:26728175-26728194 Msa0286170:three_prime_UTR 30.0%
CTTCAAAGAAGTGAAGTTAA+AGG - chr2_3:26728203-26728222 Msa0286170:three_prime_UTR 30.0%
AAAGAAGTGAAGTTAAAGGA+TGG - chr2_3:26728207-26728226 Msa0286170:three_prime_UTR 30.0%
AATTATTCTGTTGTTGCAAG+TGG - chr2_3:26728264-26728283 Msa0286170:intron 30.0%
TCATGATCATATTTAGCACT+TGG + chr2_3:26728429-26728448 None:intergenic 30.0%
ACATCCATTTAGTATTGTTG+AGG - chr2_3:26728491-26728510 Msa0286170:intron 30.0%
TCTTCCTCAACAATACTAAA+TGG + chr2_3:26728498-26728517 None:intergenic 30.0%
TTTAGTATTGTTGAGGAAGA+AGG - chr2_3:26728498-26728517 Msa0286170:intron 30.0%
AGAAGTTAAAGTCAACTTTG+AGG - chr2_3:26728619-26728638 Msa0286170:intron 30.0%
! AAATAAGATTTGCTTGACCA+CGG - chr2_3:26728647-26728666 Msa0286170:intron 30.0%
TAAAATATTTAGTGTGTCGG+TGG - chr2_3:26728848-26728867 Msa0286170:intron 30.0%
AGCTAAAAGTTCTACTTTCA+AGG - chr2_3:26728904-26728923 Msa0286170:CDS 30.0%
! TTCCATCCAACACTAATTTT+TGG + chr2_3:26728932-26728951 None:intergenic 30.0%
GACAAGCAAGTTTAGTATAT+GGG + chr2_3:26728979-26728998 None:intergenic 30.0%
TTATTCTTCCAATACCATCT+TGG + chr2_3:26729001-26729020 None:intergenic 30.0%
ATAGTTCAACAACTAAAGCT+TGG - chr2_3:26729098-26729117 Msa0286170:CDS 30.0%
AACTGACAACATTTGATACA+TGG + chr2_3:26729165-26729184 None:intergenic 30.0%
!!! ATTTTTTCTAGCTTCATCTC+CGG + chr2_3:26729564-26729583 None:intergenic 30.0%
ACATATGACCATTATCGTTA+GGG + chr2_3:26729817-26729836 None:intergenic 30.0%
!! ACCGTTGAAACTTTAATTTG+TGG - chr2_3:26730004-26730023 Msa0286170:CDS 30.0%
!! GTTGAAACTTTAATTTGTGG+TGG - chr2_3:26730007-26730026 Msa0286170:CDS 30.0%
! TTTTTATTAATAGGTGGAGC+AGG - chr2_3:26730144-26730163 Msa0286170:CDS 30.0%
TTATTCCCTTGATGTTAAGT+AGG - chr2_3:26730184-26730203 Msa0286170:CDS 30.0%
AAATTGACAAGAACCTATTG+TGG - chr2_3:26730224-26730243 Msa0286170:CDS 30.0%
AGTGATATCAAATCCACAAT+AGG + chr2_3:26730240-26730259 None:intergenic 30.0%
! AAGCTTTTGTGGATATATGT+TGG - chr2_3:26730417-26730436 Msa0286170:CDS 30.0%
! CATGCTATATTTCTAATCAG+TGG + chr2_3:26730473-26730492 None:intergenic 30.0%
ATGTATGTTGAGTAAGCTTA+TGG - chr2_3:26730490-26730509 Msa0286170:CDS 30.0%
AAGCTTATGGAATCTTCATA+AGG - chr2_3:26730503-26730522 Msa0286170:CDS 30.0%
! CTCAGCTAATTGAATTTTGT+TGG - chr2_3:26730865-26730884 Msa0286170:CDS 30.0%
ACTAACAAATGAAGCAACAC+CGG - chr2_3:26728089-26728108 Msa0286170:three_prime_UTR 35.0%
! TCATTCCCAACTTCATTTTC+CGG + chr2_3:26728111-26728130 None:intergenic 35.0%
CAACGATGCTGTTATTCAAA+AGG - chr2_3:26728146-26728165 Msa0286170:three_prime_UTR 35.0%
CCATTTGAAGAGACATTCAA+GGG - chr2_3:26728296-26728315 Msa0286170:intron 35.0%
!! TCATCAATCATGCTTTTTGC+TGG + chr2_3:26728378-26728397 None:intergenic 35.0%
ATGATTGATGAGAAGCTTTC+CGG - chr2_3:26728387-26728406 Msa0286170:intron 35.0%
TTGCTGTCTATGAATCTGAA+AGG - chr2_3:26728595-26728614 Msa0286170:intron 35.0%
! ATAGAATACATGGTGCTAAC+TGG - chr2_3:26728696-26728715 Msa0286170:intron 35.0%
! CCATTTCATTGATGCTGATT+GGG - chr2_3:26728719-26728738 Msa0286170:intron 35.0%
! AAGGCACCAAAAATTAGTGT+TGG - chr2_3:26728923-26728942 Msa0286170:CDS 35.0%
! CACCAAAAATTAGTGTTGGA+TGG - chr2_3:26728927-26728946 Msa0286170:CDS 35.0%
TTAGTATATGGGCACAACAA+CGG + chr2_3:26728968-26728987 None:intergenic 35.0%
GGACAAGCAAGTTTAGTATA+TGG + chr2_3:26728980-26728999 None:intergenic 35.0%
AGCAAGATTACGAACATTCT+CGG - chr2_3:26729073-26729092 Msa0286170:CDS 35.0%
TCTGGAAGAGTTGTATAACT+TGG + chr2_3:26729233-26729252 None:intergenic 35.0%
TCTTCCAGATGAAGATGATT+GGG - chr2_3:26729244-26729263 Msa0286170:CDS 35.0%
! TTTTCCCAATCATCTTCATC+TGG + chr2_3:26729251-26729270 None:intergenic 35.0%
GTCTAACTTCATGAAAGACA+TGG - chr2_3:26729412-26729431 Msa0286170:CDS 35.0%
! TGCTTTCCTTTAATTTACCC+CGG - chr2_3:26729542-26729561 Msa0286170:CDS 35.0%
!!! TTTTTTCTAGCTTCATCTCC+GGG + chr2_3:26729563-26729582 None:intergenic 35.0%
!! AGAACTAGTGCTGATCAAAA+TGG - chr2_3:26729668-26729687 Msa0286170:CDS 35.0%
!! GAACTAGTGCTGATCAAAAT+GGG - chr2_3:26729669-26729688 Msa0286170:CDS 35.0%
! TGAACTTCAGATTTTACAGC+AGG + chr2_3:26729767-26729786 None:intergenic 35.0%
GACATATGACCATTATCGTT+AGG + chr2_3:26729818-26729837 None:intergenic 35.0%
TGCTATTCCAATATCTACTG+TGG - chr2_3:26729913-26729932 Msa0286170:CDS 35.0%
TAGAAGAATGTAGAGAAGCT+CGG + chr2_3:26729985-26730004 None:intergenic 35.0%
!! CTTTAATTTGTGGTGGTGAT+TGG - chr2_3:26730014-26730033 Msa0286170:CDS 35.0%
GGGAATAATGTGATTTCTGT+CGG + chr2_3:26730172-26730191 None:intergenic 35.0%
ACGTACCTACTTAACATCAA+GGG + chr2_3:26730192-26730211 None:intergenic 35.0%
AACGTACCTACTTAACATCA+AGG + chr2_3:26730193-26730212 None:intergenic 35.0%
TCTCAGAAGTTCTTATCCAT+AGG + chr2_3:26730296-26730315 None:intergenic 35.0%
!!! AAGGATTTTTAGCATAGTCC+AGG - chr2_3:26730572-26730591 Msa0286170:CDS 35.0%
AGCATAACAAAAAAAGGTCC+TGG + chr2_3:26730593-26730612 None:intergenic 35.0%
CTGAAAAACAAACGAGAAAG+TGG + chr2_3:26730684-26730703 None:intergenic 35.0%
!! AAAGATTAAGTTGAAGGCTG+TGG - chr2_3:26730762-26730781 Msa0286170:CDS 35.0%
ACGTCAATTGAATCATTGGG+AGG - chr2_3:26727985-26728004 Msa0286170:three_prime_UTR 40.0%
! ATCACCAAATGATGGAGAAG+AGG - chr2_3:26728011-26728030 Msa0286170:three_prime_UTR 40.0%
! TCACCAAATGATGGAGAAGA+GGG - chr2_3:26728012-26728031 Msa0286170:three_prime_UTR 40.0%
!! GGTGTTGCTTCATTTGTTAG+TGG + chr2_3:26728090-26728109 None:intergenic 40.0%
CAACACCGGAAAATGAAGTT+GGG - chr2_3:26728103-26728122 Msa0286170:three_prime_UTR 40.0%
!! TCTTTGAAGTGATCCCAAAC+TGG + chr2_3:26728192-26728211 None:intergenic 40.0%
GCCATTTGAAGAGACATTCA+AGG - chr2_3:26728295-26728314 Msa0286170:intron 40.0%
CCCTTGAATGTCTCTTCAAA+TGG + chr2_3:26728299-26728318 None:intergenic 40.0%
! ACATTCAAGGGAAGTATGTC+AGG - chr2_3:26728308-26728327 Msa0286170:intron 40.0%
TGGATTCATAAGTAGTGGTC+CGG + chr2_3:26728409-26728428 None:intergenic 40.0%
GCACTTGGATTCATAAGTAG+TGG + chr2_3:26728414-26728433 None:intergenic 40.0%
TTTGCTTGACCACGGATTTA+TGG - chr2_3:26728655-26728674 Msa0286170:intron 40.0%
TTGTGACTTCCATAAATCCG+TGG + chr2_3:26728667-26728686 None:intergenic 40.0%
GCCATTTCATTGATGCTGAT+TGG - chr2_3:26728718-26728737 Msa0286170:intron 40.0%
CCCAATCAGCATCAATGAAA+TGG + chr2_3:26728722-26728741 None:intergenic 40.0%
! CTAAACTTGCTTGTCCAAGA+TGG - chr2_3:26728984-26729003 Msa0286170:CDS 40.0%
TTGCTTGTCCAAGATGGTAT+TGG - chr2_3:26728990-26729009 Msa0286170:CDS 40.0%
GTTCAACAACTAAAGCTTGG+TGG - chr2_3:26729101-26729120 Msa0286170:CDS 40.0%
TCGATCTTGAAAACGTGGAA+AGG + chr2_3:26729207-26729226 None:intergenic 40.0%
CTCTTCCAGATGAAGATGAT+TGG - chr2_3:26729243-26729262 Msa0286170:CDS 40.0%
AAAAGTTTGCAAGCTGCTAG+AGG - chr2_3:26729277-26729296 Msa0286170:CDS 40.0%
GGTGTTTCAAATCAAGCAAG+TGG - chr2_3:26729367-26729386 Msa0286170:CDS 40.0%
! TATGTCCCTTGCTTCTGTTT+TGG - chr2_3:26729487-26729506 Msa0286170:CDS 40.0%
GACCCTAGGATCAAAATGAT+GGG - chr2_3:26729509-26729528 Msa0286170:CDS 40.0%
!! CTCCCATCATTTTGATCCTA+GGG + chr2_3:26729514-26729533 None:intergenic 40.0%
!! ACTCCCATCATTTTGATCCT+AGG + chr2_3:26729515-26729534 None:intergenic 40.0%
TCATCTCCGGGGTAAATTAA+AGG + chr2_3:26729551-26729570 None:intergenic 40.0%
!!! TTTTTCTAGCTTCATCTCCG+GGG + chr2_3:26729562-26729581 None:intergenic 40.0%
GAAGCATATCAGCATACTCT+AGG + chr2_3:26729622-26729641 None:intergenic 40.0%
AGAGTATGCTGATATGCTTC+AGG - chr2_3:26729622-26729641 Msa0286170:CDS 40.0%
!! TGATCAAAATGGGCTGACAT+TGG - chr2_3:26729679-26729698 Msa0286170:CDS 40.0%
TTATCGTTAGGGCTGTAAGT+TGG + chr2_3:26729806-26729825 None:intergenic 40.0%
ACTTACAGCCCTAACGATAA+TGG - chr2_3:26729806-26729825 Msa0286170:CDS 40.0%
! TCATATGTCCTTTTGTGCCT+TGG - chr2_3:26729829-26729848 Msa0286170:CDS 40.0%
ATTCAACTTCCACCATTCCA+AGG + chr2_3:26729849-26729868 None:intergenic 40.0%
! GAATGGTGGAAGTTGAATAG+CGG - chr2_3:26729851-26729870 Msa0286170:CDS 40.0%
! TCTGAAGCCACAGTAGATAT+TGG + chr2_3:26729923-26729942 None:intergenic 40.0%
! ATTGGCTTCGAGTATTGCAT+GGG - chr2_3:26730032-26730051 Msa0286170:CDS 40.0%
TGCTCATTGTTCTTGACCTA+TGG - chr2_3:26730277-26730296 Msa0286170:CDS 40.0%
! CTGTGATGCATAAGCTTTTG+TGG - chr2_3:26730406-26730425 Msa0286170:CDS 40.0%
GACATTTACGGGCATGTATA+AGG - chr2_3:26730553-26730572 Msa0286170:CDS 40.0%
GGAGGTTCATCACCAAATGA+TGG - chr2_3:26728003-26728022 Msa0286170:three_prime_UTR 45.0%
AAGCCCTCTTCTCCATCATT+TGG + chr2_3:26728018-26728037 None:intergenic 45.0%
GCAACACCGGAAAATGAAGT+TGG - chr2_3:26728102-26728121 Msa0286170:three_prime_UTR 45.0%
CATCATAACCCAATGTGCAG+TGG + chr2_3:26728466-26728485 None:intergenic 45.0%
! AAAAAGTGCGTGACAGTGTG+AGG - chr2_3:26729033-26729052 Msa0286170:CDS 45.0%
GGTTCTCGATCTTGAAAACG+TGG + chr2_3:26729212-26729231 None:intergenic 45.0%
AGACATGGCAAATGCGATGA+AGG - chr2_3:26729427-26729446 Msa0286170:CDS 45.0%
AGGGTCCAAAACAGAAGCAA+GGG + chr2_3:26729495-26729514 None:intergenic 45.0%
TAGGGTCCAAAACAGAAGCA+AGG + chr2_3:26729496-26729515 None:intergenic 45.0%
!! TTGCTTCTGTTTTGGACCCT+AGG - chr2_3:26729495-26729514 Msa0286170:CDS 45.0%
GGACCCTAGGATCAAAATGA+TGG - chr2_3:26729508-26729527 Msa0286170:CDS 45.0%
! CTTCAGGAGCATAGAGAAGA+AGG - chr2_3:26729638-26729657 Msa0286170:CDS 45.0%
! TGTCCTTTTGTGCCTTGGAA+TGG - chr2_3:26729834-26729853 Msa0286170:CDS 45.0%
! TCAGAGTCAACTTTTAGCGC+CGG - chr2_3:26729938-26729957 Msa0286170:CDS 45.0%
!! GATTGGCTTCGAGTATTGCA+TGG - chr2_3:26730031-26730050 Msa0286170:CDS 45.0%
!!! TATCAATATTTTTTATTAAT+AGG - chr2_3:26730135-26730154 Msa0286170:CDS 5.0%
AACGAGAAGCCACTGCACAT+TGG - chr2_3:26728454-26728473 Msa0286170:intron 50.0%
ACGAGAAGCCACTGCACATT+GGG - chr2_3:26728455-26728474 Msa0286170:intron 50.0%
ATATCAACACCACGTCGAGG+AGG + chr2_3:26728783-26728802 None:intergenic 50.0%
GCAATATCAACACCACGTCG+AGG + chr2_3:26728786-26728805 None:intergenic 50.0%
TTCTTGATTGCCCTACTCGC+TGG - chr2_3:26729135-26729154 Msa0286170:CDS 50.0%
ACATGGAGTTCCAGCGAGTA+GGG + chr2_3:26729148-26729167 None:intergenic 50.0%
TACATGGAGTTCCAGCGAGT+AGG + chr2_3:26729149-26729168 None:intergenic 50.0%
TTGGAGCCAACAAGAGACAC+AGG - chr2_3:26729698-26729717 Msa0286170:CDS 50.0%
TGGAGCCAACAAGAGACACA+GGG - chr2_3:26729699-26729718 Msa0286170:CDS 50.0%
CCACCATTCCAAGGCACAAA+AGG + chr2_3:26729840-26729859 None:intergenic 50.0%
! CCTTTTGTGCCTTGGAATGG+TGG - chr2_3:26729837-26729856 Msa0286170:CDS 50.0%
! CAGAGTCAACTTTTAGCGCC+GGG - chr2_3:26729939-26729958 Msa0286170:CDS 50.0%
! AGAGTCAACTTTTAGCGCCG+GGG - chr2_3:26729940-26729959 Msa0286170:CDS 50.0%
TGAGTCAATAACTCTCCCCC+CGG + chr2_3:26729960-26729979 None:intergenic 50.0%
GTTGAAGGCTGTGGCAAATG+TGG - chr2_3:26730771-26730790 Msa0286170:CDS 50.0%
GTCTTGAAGGGGAGAGCAAT+TGG - chr2_3:26730808-26730827 Msa0286170:CDS 50.0%
AGGGGAGAGCAATTGGAGTT+TGG - chr2_3:26730815-26730834 Msa0286170:CDS 50.0%
AGCCAACAAGAGACACAGGG+TGG - chr2_3:26729702-26729721 Msa0286170:CDS 55.0%
GACCACCCTGTGTCTCTTGT+TGG + chr2_3:26729707-26729726 None:intergenic 55.0%
!! GAGTCAACTTTTAGCGCCGG+GGG - chr2_3:26729941-26729960 Msa0286170:CDS 55.0%
!! AGTCAACTTTTAGCGCCGGG+GGG - chr2_3:26729942-26729961 Msa0286170:CDS 55.0%
CATGTTCCTCCTCCTCGACG+TGG - chr2_3:26728771-26728790 Msa0286170:CDS 60.0%
TCAACACCACGTCGAGGAGG+AGG + chr2_3:26728780-26728799 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 26727973 26730976 26727973 ID=Msa0286170;Name=Msa0286170
chr2_3 mRNA 26727973 26730976 26727973 ID=Msa0286170-mRNA-1;Parent=Msa0286170;Name=Msa0286170-mRNA-1;_AED=0.05;_eAED=0.18;_QI=0|1|0.66|1|0.5|0.33|3|283|712
chr2_3 exon 26727973 26728245 26727973 ID=Msa0286170-mRNA-1:exon:9730;Parent=Msa0286170-mRNA-1
chr2_3 exon 26728744 26728792 26728744 ID=Msa0286170-mRNA-1:exon:9729;Parent=Msa0286170-mRNA-1
chr2_3 exon 26728877 26730976 26728877 ID=Msa0286170-mRNA-1:exon:9728;Parent=Msa0286170-mRNA-1
chr2_3 CDS 26728877 26730976 26728877 ID=Msa0286170-mRNA-1:cds;Parent=Msa0286170-mRNA-1
chr2_3 CDS 26728754 26728792 26728754 ID=Msa0286170-mRNA-1:cds;Parent=Msa0286170-mRNA-1
chr2_3 three_prime_UTR 26728744 26728753 26728744 ID=Msa0286170-mRNA-1:three_prime_utr;Parent=Msa0286170-mRNA-1
chr2_3 three_prime_UTR 26727973 26728245 26727973 ID=Msa0286170-mRNA-1:three_prime_utr;Parent=Msa0286170-mRNA-1
Gene Sequence

>Msa0286170

ATGTCAGCTAGTACGTCAATTGAATCATTGGGAGGTTCATCACCAAATGATGGAGAAGAGGGCTTAAGTTTGTTGAATCATATCATTGACTTAGATGCGAATACAAACGAAGAACCACTAACAAATGAAGCAACACCGGAAAATGAAGTTGGGAATGAAGAGAATGTCGAGAACAACGATGCTGTTATTCAAAAGGCTAAGAGAAATAAAACTTCTCCAGTTTGGGATCACTTCAAAGAAGTGAAGTTAAAGGATGGAACAAAAAAATGGCAATGTTTACATTGTAAAAGAAATTATTCTGTTGTTGCAAGTGGATCGACTAGCCATTTGAAGAGACATTCAAGGGAAGTATGTCAGGTTTATAAGAAGCTAGCAGCACAACAACAAAAATTAAACTTGAAGCCAGCAAAAAGCATGATTGATGAGAAGCTTTCCGGACCACTACTTATGAATCCAAGTGCTAAATATGATCATGAGAGACAACGAGAAGCCACTGCACATTGGGTTATGATGCATGAACATCCATTTAGTATTGTTGAGGAAGAAGGTTTTCTTTTTATGATGAAATGTTCTAATTTTTCATATGAGAAAATTAGTCGGAAAACATTGAAGAATGATTGTGTTGCTGTCTATGAATCTGAAAGGAAGAAGTTAAAGTCAACTTTGAGGACAATAAATAAGATTTGCTTGACCACGGATTTATGGAAGTCACAAAATCAAAAGATAGAATACATGGTGCTAACTGGCCATTTCATTGATGCTGATTGGGTTTTGCAAAAGCGCGTTCTCAGTTTTGTTCATGTTCCTCCTCCTCGACGTGGTGTTGATATTGCTGATGCAATCTTTAAATGTCTCAAAGAATGGGGCATTGAAAATAAAATATTTAGTGTGTCGGTGGATAATGCATACTACAACGATAGATGTTTGAAAGAGCTAAAAGTTCTACTTTCAAGGCACCAAAAATTAGTGTTGGATGGAAAGTTGTTTCATGTCCGTTGTTGTGCCCATATACTAAACTTGCTTGTCCAAGATGGTATTGGAAGAATAAGAGAAATAGTTGAAAAAGTGCGTGACAGTGTGAGGTTTATCAATCAGTCTGAAGCAAGATTACGAACATTCTCGGAAATAGTTCAACAACTAAAGCTTGGTGGTAAAAAATTAATTCTTGATTGCCCTACTCGCTGGAACTCCATGTATCAAATGTTGTCAGTTGCAATGCAGTTCAAAGAAGCCTTTCCACGTTTTCAAGATCGAGAACCAAGTTATACAACTCTTCCAGATGAAGATGATTGGGAAAAAGTTGAAAAAGTTTGCAAGCTGCTAGAGGTATTTAATTCTGTTACAAATATAATTTCTGGTAGTGAATATCCAACTTCTAATTTATATCTTGCTGAGGTGTTTCAAATCAAGCAAGTGGTAGATCAAGCTGTTCAAGATGAGTCTAACTTCATGAAAGACATGGCAAATGCGATGAAGGAAAAATTTGACAAGTATTGGAGTCAGTGTAATCTTGTTATGTCCCTTGCTTCTGTTTTGGACCCTAGGATCAAAATGATGGGAGTAAACATGTGCTTTCCTTTAATTTACCCCGGAGATGAAGCTAGAAAAAATATAGAAAAGGTGCACAAGACTCTTAATGATATGTACCTAGAGTATGCTGATATGCTTCAGGAGCATAGAGAAGAAGGCTCTAGTAGAACTAGTGCTGATCAAAATGGGCTGACATTGGAGCCAACAAGAGACACAGGGTGGTCAAAGTTAATGAATTATGTCAAAGAACAACAAGCAATTCCTGCTGTAAAATCTGAAGTTCAAGAGTATCTGAATGAGCCAACTTACAGCCCTAACGATAATGGTCATATGTCCTTTTGTGCCTTGGAATGGTGGAAGTTGAATAGCGGAAAATATAGAGTATTATCTCATATGGCAGCAGATGTTCTTGCTATTCCAATATCTACTGTGGCTTCAGAGTCAACTTTTAGCGCCGGGGGGAGAGTTATTGACTCATTCCGAGCTTCTCTACATTCTTCTACCGTTGAAACTTTAATTTGTGGTGGTGATTGGCTTCGAGTATTGCATGGGATTAGAAACAAGCCTAAAGTGGAGCAGGTTCCGACAGAAATCACATTATTCCCTTGA
Protein sequence

>Msa0286170

MSASTSIESLGGSSPNDGEEGLSLLNHIIDLDANTNEEPLTNEATPENEVGNEENVENNDAVIQKAKRNKTSPVWDHFKEVKLKDGTKKWQCLHCKRNYSVVASGSTSHLKRHSREVCQVYKKLAAQQQKLNLKPAKSMIDEKLSGPLLMNPSAKYDHERQREATAHWVMMHEHPFSIVEEEGFLFMMKCSNFSYEKISRKTLKNDCVAVYESERKKLKSTLRTINKICLTTDLWKSQNQKIEYMVLTGHFIDADWVLQKRVLSFVHVPPPRRGVDIADAIFKCLKEWGIENKIFSVSVDNAYYNDRCLKELKVLLSRHQKLVLDGKLFHVRCCAHILNLLVQDGIGRIREIVEKVRDSVRFINQSEARLRTFSEIVQQLKLGGKKLILDCPTRWNSMYQMLSVAMQFKEAFPRFQDREPSYTTLPDEDDWEKVEKVCKLLEVFNSVTNIISGSEYPTSNLYLAEVFQIKQVVDQAVQDESNFMKDMANAMKEKFDKYWSQCNLVMSLASVLDPRIKMMGVNMCFPLIYPGDEARKNIEKVHKTLNDMYLEYADMLQEHREEGSSRTSADQNGLTLEPTRDTGWSKLMNYVKEQQAIPAVKSEVQEYLNEPTYSPNDNGHMSFCALEWWKLNSGKYRVLSHMAADVLAIPISTVASESTFSAGGRVIDSFRASLHSSTVETLICGGDWLRVLHGIRNKPKVEQVPTEITLFP*