AlfalfaGEDB | Msa0289850

M. sativa cultivar zhongmu-4 / Msa0289850

KEGG: -
GO: -
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0289850	GAU20217.1	65.110	728	247	3	31	753	3	728	0.0	1031

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0289850	sp\|Q9SZL8\|FRS5_ARATH	30.420	572	386	7	72	634	69	637	2.59e-91	306

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0289850	A0A445I407	50.393	764	367	5	6	768	61	813	0.0	803

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TFs/TRs:

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Gene ID	Type	Classification
Msa0289850	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0210650	Msa0289850	0.999559	1.037538e-322	-8.615850e-47
Msa0210700	Msa0289850	0.961682	5.500835e-120	-8.615850e-47
Msa0279920	Msa0289850	0.988332	1.317996e-173	-8.615850e-47
Msa0289850	Msa0728840	0.852378	4.802672e-61	-8.615850e-47
Msa0289850	Msa0777700	0.866049	3.786274e-65	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0289850	MtrunA17_Chr1g0203051	44.444	603	322	4	26	618	22	621	0.0	570
Msa0289850	MtrunA17_Chr2g0287651	35.123	689	435	5	67	753	66	744	4.54e-148	456
Msa0289850	MtrunA17_Chr2g0308841	70.354	226	67	0	405	630	1	226	6.49e-120	361
Msa0289850	MtrunA17_Chr5g0448761	31.866	568	375	6	78	634	62	628	3.41e-99	325
Msa0289850	MtrunA17_Chr1g0202341	31.327	565	375	6	74	630	49	608	6.19e-97	316
Msa0289850	MtrunA17_Chr2g0324011	30.054	559	374	7	82	630	16	567	5.97e-85	281
Msa0289850	MtrunA17_Chr8g0363841	29.735	565	384	7	86	641	27	587	6.40e-80	270
Msa0289850	MtrunA17_Chr1g0198761	27.865	768	505	20	30	768	1	748	4.82e-79	270
Msa0289850	MtrunA17_Chr1g0203721	27.812	640	412	14	14	630	10	622	3.70e-78	270
Msa0289850	MtrunA17_Chr5g0441581	26.412	655	426	15	82	703	38	669	9.46e-75	256

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0289850	AT4G38180.1	30.420	572	386	7	72	634	69	637	2.63e-92	306
Msa0289850	AT3G06250.2	29.749	558	337	12	80	630	194	703	1.32e-72	253
Msa0289850	AT3G06250.1	29.749	558	337	12	80	630	194	703	1.32e-72	253
Msa0289850	AT3G06250.3	29.749	558	337	12	80	630	194	703	1.32e-72	253
Msa0289850	AT5G18960.2	29.537	562	336	14	80	630	215	727	7.13e-71	248
Msa0289850	AT5G18960.1	29.537	562	336	14	80	630	215	727	7.13e-71	248
Msa0289850	AT3G22170.2	25.872	688	449	16	22	684	14	665	1.71e-69	245
Msa0289850	AT3G22170.1	25.872	688	449	16	22	684	14	665	1.71e-69	245
Msa0289850	AT4G15090.1	26.821	604	424	10	81	675	56	650	3.90e-68	241
Msa0289850	AT4G15090.5	26.821	604	424	10	81	675	56	650	3.90e-68	241

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Find 153 sgRNAs with CRISPR-Local

Find 362 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATTAGTTTCATCTGACTT+TGG	0.470983	2_3:-36259168	Msa0289850:CDS
AACATCACCGTCAGTTATTA+CGG	0.364947	2_3:+36258816	None:intergenic
AACTTTGTAGATAGAACCCA+TGG	0.589394	2_3:+36258222	None:intergenic
AAGAATAAACCAACAGCTGT+TGG	0.571265	2_3:-36257836	Msa0289850:CDS
AAGATGCATGTTGGGTGATA+TGG	0.328276	2_3:-36258674	Msa0289850:CDS
AAGGATTTGTATAACCAATC+AGG	0.433589	2_3:-36259201	Msa0289850:CDS
AATAAACGATCATGTCCTAA+AGG	0.523227	2_3:-36257656	Msa0289850:CDS
AATCCAATTGCCACGCCTGG+TGG	0.547004	2_3:-36257725	Msa0289850:CDS
AATGGGGCTAATGAGTGTGA+TGG	0.645283	2_3:-36259804	Msa0289850:CDS
ACACAAATGGTTCACAATAT+TGG	0.219824	2_3:-36257988	Msa0289850:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CAATATTAACATCGAGGAAA+TGG	-	chr2_3:36257606-36257625	Msa0289850:CDS	30.0%
GAGATAATATGGATGACAAT+GGG	-	chr2_3:36257647-36257666	Msa0289850:CDS	30.0%
AGATAATATGGATGACAATG+GGG	-	chr2_3:36257648-36257667	Msa0289850:CDS	30.0%
TTTCTGTAGATGTAGTTGAA+AGG	-	chr2_3:36257782-36257801	Msa0289850:CDS	30.0%
GTTATGCGCAAATTCAAATA+CGG	+	chr2_3:36258076-36258095	None:intergenic	30.0%
AAGGATTTGTATAACCAATC+AGG	-	chr2_3:36258267-36258286	Msa0289850:CDS	30.0%
GTATGTGCAACATACATTAA+AGG	+	chr2_3:36258366-36258385	None:intergenic	30.0%
ATACTGTAGACAAAGATAAG+AGG	-	chr2_3:36258382-36258401	Msa0289850:CDS	30.0%
TTTCCTATAAGTTGCATCAA+AGG	+	chr2_3:36258475-36258494	None:intergenic	30.0%
TTGGTTATGATGATTAACAC+CGG	+	chr2_3:36258529-36258548	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_3	gene	36257568	36259922	36257568	ID=Msa0289850;Name=Msa0289850
chr2_3	mRNA	36257568	36259922	36257568	ID=Msa0289850-mRNA-1;Parent=Msa0289850;Name=Msa0289850-mRNA-1;_AED=0.08;_eAED=0.09;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|784
chr2_3	exon	36257568	36259922	36257568	ID=Msa0289850-mRNA-1:exon:11918;Parent=Msa0289850-mRNA-1
chr2_3	CDS	36257568	36259922	36257568	ID=Msa0289850-mRNA-1:cds;Parent=Msa0289850-mRNA-1

Gene Sequence

>Msa0289850

ATGGAGGAGGTAACAGAAAGTTCAGCTGACAGTAATGACAATATTAACATCGAGGAAATGGACGAAGCTTCAGACGAAGGAGATAATATGGATGACAATGGGGCTAATGAGTGTGATGGAGATTTAGAAGCGAGTAGCGATTCATCCGAGTCTAATGGGAATGCTATAGTCATAAATGGCATCGGTGATATTTTAAAGATGGACATGAAAAACATTTCTGTAGATGTAGTTGAAAGGTTTGACTTTGCTGATTTAGATGTTGCATATAATTTCTACTCATGGTATGCAAGGGTAAACGGATTTTCTGTTCGGAAGACCCAAGTTCTGAGGGACCGTTTGGGTAATGTTCTGCAGCAAACTTTTGTTTGTTCGCGGGAAGGTTTTAGAGAGGACAGGGGATTGACCTTGGAGAGGCGGAAGCGAGAACCCAGAAATGATACCAGGTGTGGATGTTCTGCAAAATGTCGTGTCCACATAGACATTGTTTCGAAGCGTTGGTATGTGACCGTATTTGAATTTGCGCATAACCACGAGTTGTTGGATGGTTTGCTATGTGGTCTTTTACCGGCACATAGAAAGCTAACAGCAACTGATTTGATTAAGATTGAAAAGTATAAGAATGTTGGTGTAAGACCATATCAAACGTATGGTGCAATGGCTAATTCATCAGGTGGTTTTCACAAGTTAGGTTTTGTGAAAAAGGATTTGTATAACCAATCAGGAAGACAAAGGAAATTAGTTTCATCTGACTTTGGCGGTGCACTGAAGTATCTTCATATGCTTCGTACAAAAGACCCTTTAATGTATGTTGCACATACTGTAGACAAAGATAAGAGGCTTGAACGTCTTTTTTGGTGTGATGGAGAAAGTCGTATGAATTATGAAATTTTTGGTGATGTATTGGCCTTTGATGCAACTTATAGGAAAAACAAATACAATTGCCCGTTTGTTATTTTCTCCGGTGTTAATCATCATAACCAAACTGTTGTCTTTGCGACAGGTTTAGTTACGAGAGAGACGGAAGAGACATATGTTTGGTTGTTGGACCAATTTGCGACTGCTATGAATGGTAAGGCTCCTTTATCCGTAATAACTGACGGTGATGTTGCAATGAAAAATGCCATAAAGAGTATATTTCCAAAAGCACATCATAGATTATGTGCTTGGCATCTTCTACGGAATGCATCATCGAATGTTGGGATTGCCGATTTCATGTCATATCTTAAAAGATGCATGTTGGGTGATATGGAGGTGAATAAGTTTGAAGAACTATGGGAAGAAATGGTTGAAAAATTTGGCCTGCAAGACAACAATTGGATCAATGAGATGTATGCAAAAAAAAGAATGTGGGCGACTGCTCATATCAAAGGAGAATTTTTTGCAGGTATACGAACCACATCTCGGTGTGAGGGTTTACATAGTCACATAGGTCAATTTGTGCATTCAAGAATTAACTTGACTGATTTTGTCCAGCAATTTCATAGATGCCTTACATATTTTCGATTTCGAGAGATTGAGTCCGACTATCGTTCCAACTATGGTCAACCCGTATTGCAAACTAGTTTGCGATTCATTGAGAGATCAGCATCGAACCAGTTTACAAGGGAGATTTTCTTCCTTTTCCGTAATGCTCTGAAAAAATCTTTGATGTTGAGAATTATTGAAATTCAAGAGATGTCCATGGGTTCTATCTACAAAGTTTCAAAGTACTGTGGAAATGGAAGTGTCTGGCACGTTACACATTGTGAAACTCCTGTAGATTTCAAATGTTCGTGTCTTAGGATGGAGTCGTTTGGACTGCCTTGCGATCATATTATTGCTGTTTTGATGTATCTGGATTTTGATCACCTTCCCAAGAATCTAGTTTTACACAGGTGGACAAAGTTCGCTACAGATACAGTTCGAGCAAGATACACAAATGGTTCACAATATTGGGATTCTCAAATTGCTTCAAGGTATGCGGGAATTATTCAGTTGTCGAAGGATGTGGCTGAGAGGGTTTATCAAGATGTAGAGGAGTACAACCGCATTGTAGATTTTCTTAGTGCTGAACTTTTGAGGTTGAAGAATAAACCAACAGCTGTTGGTATTGATGCTAGCACTGGGGTACACAATGTAGTCGTCGACGAAAGTTTACTGGACCCTACTTGTGTGAGGACTAAAGGGTGTGGATCTAATCCAATTGCCACGCCTGGTGGAAGTAGGCGTCCTCAATCATGTAGTCAGTGTGGTGTGATAGGACACAATAAACGATCATGTCCTAAAGGAGTCAACAACTTTGAAAATGCATGCACTTCGAGTGCGACAATTTATGAAATTAATGAAGATGATGAAGAAGGAGATCTTGAGCTGTGA

Protein sequence

>Msa0289850

MEEVTESSADSNDNINIEEMDEASDEGDNMDDNGANECDGDLEASSDSSESNGNAIVINGIGDILKMDMKNISVDVVERFDFADLDVAYNFYSWYARVNGFSVRKTQVLRDRLGNVLQQTFVCSREGFREDRGLTLERRKREPRNDTRCGCSAKCRVHIDIVSKRWYVTVFEFAHNHELLDGLLCGLLPAHRKLTATDLIKIEKYKNVGVRPYQTYGAMANSSGGFHKLGFVKKDLYNQSGRQRKLVSSDFGGALKYLHMLRTKDPLMYVAHTVDKDKRLERLFWCDGESRMNYEIFGDVLAFDATYRKNKYNCPFVIFSGVNHHNQTVVFATGLVTRETEETYVWLLDQFATAMNGKAPLSVITDGDVAMKNAIKSIFPKAHHRLCAWHLLRNASSNVGIADFMSYLKRCMLGDMEVNKFEELWEEMVEKFGLQDNNWINEMYAKKRMWATAHIKGEFFAGIRTTSRCEGLHSHIGQFVHSRINLTDFVQQFHRCLTYFRFREIESDYRSNYGQPVLQTSLRFIERSASNQFTREIFFLFRNALKKSLMLRIIEIQEMSMGSIYKVSKYCGNGSVWHVTHCETPVDFKCSCLRMESFGLPCDHIIAVLMYLDFDHLPKNLVLHRWTKFATDTVRARYTNGSQYWDSQIASRYAGIIQLSKDVAERVYQDVEEYNRIVDFLSAELLRLKNKPTAVGIDASTGVHNVVVDESLLDPTCVRTKGCGSNPIATPGGSRRPQSCSQCGVIGHNKRSCPKGVNNFENACTSSATIYEINEDDEEGDLEL*