AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0777700

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0777700 MtrunA17_Chr1g0203051 44.113 603 324 4 63 655 22 621 0.0 567
Msa0777700 MtrunA17_Chr2g0287651 34.833 689 437 4 104 790 66 744 1.13e-146 453
Msa0777700 MtrunA17_Chr2g0308841 70.354 226 67 0 442 667 1 226 2.49e-119 361
Msa0777700 MtrunA17_Chr5g0448761 31.866 568 375 6 115 671 62 628 3.41e-98 323
Msa0777700 MtrunA17_Chr1g0202341 29.794 631 417 9 111 725 49 669 8.20e-97 316
Msa0777700 MtrunA17_Chr2g0324011 30.411 559 372 8 119 667 16 567 9.43e-85 281
Msa0777700 MtrunA17_Chr8g0363841 28.681 652 441 11 123 756 27 672 2.78e-79 269
Msa0777700 MtrunA17_Chr1g0203721 27.812 640 412 14 51 667 10 622 3.76e-77 268
Msa0777700 MtrunA17_Chr1g0198761 27.928 777 493 22 67 805 1 748 6.74e-77 265
Msa0777700 MtrunA17_Chr5g0441581 27.972 572 358 12 119 665 38 580 1.45e-74 256
Msa0777700 MtrunA17_Chr6g0458391 28.475 590 371 13 97 667 207 764 2.95e-71 249
Msa0777700 MtrunA17_Chr6g0458391 29.333 225 135 10 24 239 5 214 6.85e-14 75.9
Msa0777700 MtrunA17_Chr2g0287061 30.782 601 347 19 89 671 155 704 1.00e-70 248
Msa0777700 MtrunA17_Chr2g0296781 29.130 563 371 12 128 676 12 560 5.78e-69 242
Msa0777700 MtrunA17_Chr5g0440451 27.496 611 395 13 119 702 49 638 3.67e-67 240
Msa0777700 MtrunA17_Chr5g0448511 29.771 393 269 3 277 664 3 393 2.27e-66 230
Msa0777700 MtrunA17_Chr1g0207551 28.929 560 378 12 118 667 39 588 2.13e-65 233
Msa0777700 MtrunA17_Chr3g0118611 29.139 453 309 6 269 712 96 545 2.12e-64 230
Msa0777700 MtrunA17_Chr6g0477261 30.088 452 295 8 239 676 5 449 8.79e-63 223
Msa0777700 MtrunA17_Chr3g0082121 26.601 609 403 13 82 667 2 589 1.16e-62 224
Msa0777700 MtrunA17_Chr2g0329031 27.350 585 389 14 106 678 7 567 2.85e-61 221
Msa0777700 MtrunA17_Chr3g0080351 26.003 623 415 12 63 667 28 622 7.90e-61 221
Msa0777700 MtrunA17_Chr5g0431001 31.504 419 262 8 119 517 67 480 1.72e-60 213
Msa0777700 MtrunA17_Chr2g0276841 27.163 578 377 15 119 672 76 633 1.24e-57 209
Msa0777700 MtrunA17_Chr2g0290391 25.825 697 436 19 63 729 25 670 6.68e-55 201
Msa0777700 MtrunA17_Chr2g0291581 24.042 574 379 13 118 665 76 618 9.59e-55 201
Msa0777700 MtrunA17_Chr1g0196541 29.197 411 276 5 276 676 155 560 1.17e-53 199
Msa0777700 MtrunA17_Chr2g0291591 27.959 490 309 11 114 582 65 531 7.83e-52 190
Msa0777700 MtrunA17_Chr2g0294841 26.612 605 405 15 86 663 11 603 4.69e-51 191
Msa0777700 MtrunA17_Chr1g0164591 27.796 313 212 5 194 500 3 307 2.62e-36 140
Msa0777700 MtrunA17_Chr8g0389811 33.796 216 127 2 19 234 6 205 1.31e-35 134
Msa0777700 MtrunA17_Chr1g0171741 35.556 225 120 5 15 239 59 258 5.36e-34 131
Msa0777700 MtrunA17_Chr2g0331371 48.592 142 69 1 77 218 23 160 2.71e-33 127
Msa0777700 MtrunA17_Chr7g0258861 29.054 296 198 6 112 401 11 300 7.45e-33 129
Msa0777700 MtrunA17_Chr6g0473421 44.444 117 61 1 77 193 23 135 1.56e-26 106
Msa0777700 MtrunA17_Chr1g0172931 44.231 104 58 0 92 195 55 158 1.64e-24 100
Msa0777700 MtrunA17_Chr8g0340801 40.000 105 63 0 224 328 1 105 3.66e-23 95.1
Msa0777700 MtrunA17_Chr1g0193171 21.805 399 245 14 274 649 304 658 1.84e-12 71.2
Msa0777700 MtrunA17_Chr1g0149131 28.182 110 74 1 392 496 1 110 9.54e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0777700 AT4G38180.1 30.851 564 378 7 117 671 77 637 2.60e-91 305
Msa0777700 AT3G06250.2 29.391 558 339 12 117 667 194 703 7.68e-71 248
Msa0777700 AT3G06250.1 29.391 558 339 12 117 667 194 703 7.68e-71 248
Msa0777700 AT3G06250.3 29.391 558 339 12 117 667 194 703 7.68e-71 248
Msa0777700 AT5G18960.2 29.359 562 337 14 117 667 215 727 6.72e-70 246
Msa0777700 AT5G18960.1 29.359 562 337 14 117 667 215 727 6.72e-70 246
Msa0777700 AT4G15090.1 27.915 566 391 9 118 673 56 614 1.06e-68 243
Msa0777700 AT4G15090.5 27.915 566 391 9 118 673 56 614 1.06e-68 243
Msa0777700 AT4G15090.3 27.915 566 391 9 118 673 56 614 1.06e-68 243
Msa0777700 AT4G15090.2 27.915 566 391 9 118 673 56 614 1.06e-68 243
Msa0777700 AT4G15090.4 27.915 566 391 9 118 673 56 614 1.06e-68 243
Msa0777700 AT3G22170.2 25.980 689 447 16 59 721 14 665 2.77e-67 239
Msa0777700 AT3G22170.1 25.980 689 447 16 59 721 14 665 2.77e-67 239
Msa0777700 AT1G76320.3 28.750 560 371 11 118 667 2 543 1.27e-63 226
Msa0777700 AT1G76320.1 28.750 560 371 11 118 667 2 543 1.64e-63 227
Msa0777700 AT1G76320.4 28.750 560 371 11 118 667 2 543 1.64e-63 227
Msa0777700 AT1G76320.2 28.750 560 371 11 118 667 2 543 1.67e-63 227
Msa0777700 AT2G27110.2 26.246 602 399 11 76 671 20 582 3.08e-63 228
Msa0777700 AT2G27110.1 26.246 602 399 11 76 671 20 582 3.08e-63 228
Msa0777700 AT2G27110.3 28.283 396 276 3 281 671 45 437 2.74e-61 220
Msa0777700 AT1G10240.2 29.066 578 378 16 119 678 54 617 5.52e-60 216
Msa0777700 AT1G10240.1 29.066 578 378 16 119 678 54 617 5.52e-60 216
Msa0777700 AT2G32250.5 27.256 554 351 11 118 665 43 550 4.51e-59 215
Msa0777700 AT2G32250.2 27.256 554 351 11 118 665 43 550 6.13e-59 215
Msa0777700 AT2G32250.4 27.256 554 351 11 118 665 43 550 6.13e-59 215
Msa0777700 AT2G32250.8 27.256 554 351 11 118 665 2 509 6.44e-59 214
Msa0777700 AT2G32250.6 27.256 554 351 11 118 665 43 550 6.97e-59 215
Msa0777700 AT2G32250.1 27.256 554 351 11 118 665 43 550 6.97e-59 215
Msa0777700 AT2G32250.3 27.256 554 351 11 118 665 43 550 6.97e-59 215
Msa0777700 AT2G32250.7 27.256 554 351 11 118 665 2 509 7.42e-59 214
Msa0777700 AT1G80010.1 28.934 591 371 17 118 678 100 671 1.25e-56 207
Msa0777700 AT1G52520.1 26.758 583 355 16 118 665 90 635 4.48e-54 200
Msa0777700 AT4G19990.5 25.153 652 421 14 105 732 13 621 2.46e-52 195
Msa0777700 AT4G19990.3 25.153 652 421 14 105 732 13 621 2.63e-52 195
Msa0777700 AT4G19990.4 25.153 652 421 14 105 732 13 621 2.63e-52 195
Msa0777700 AT4G19990.7 25.153 652 421 14 105 732 27 635 3.42e-52 195
Msa0777700 AT4G19990.6 25.153 652 421 14 105 732 13 621 3.93e-52 195
Msa0777700 AT4G19990.2 25.153 652 421 14 105 732 13 621 3.93e-52 195
Msa0777700 AT4G38170.1 27.027 370 260 4 320 680 46 414 5.29e-50 185
Msa0777700 AT5G28530.3 26.095 571 381 14 119 665 61 614 2.05e-49 186
Msa0777700 AT5G28530.2 26.095 571 381 14 119 665 61 614 2.05e-49 186
Msa0777700 AT5G28530.1 26.270 571 384 14 119 665 61 618 2.14e-49 186
Msa0777700 AT4G19990.1 25.124 605 387 13 151 732 3 564 3.61e-48 182
Msa0777700 AT5G28530.4 24.373 558 344 16 119 663 61 553 2.51e-31 130
Msa0777700 AT5G28530.5 24.194 558 341 16 119 663 61 549 3.22e-31 130

Find 160 sgRNAs with CRISPR-Local

Find 243 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCACACCTGGTATCATTTC+TGG 0.155992 5_2:+56993897 None:intergenic
CCGTCAGTTATTACGGTTAA+AGG 0.199417 5_2:+56993247 None:intergenic
ACACAAATGGTTCACAATAT+TGG 0.219824 5_2:-56992412 Msa0777700:CDS
CACAAATGGTTCACAATATT+GGG 0.257023 5_2:-56992411 Msa0777700:CDS
CATAATCTATGATGTGCTTT+TGG 0.261343 5_2:+56993187 None:intergenic
TGATCCAATTGTTGTCTTGC+AGG 0.279613 5_2:+56993026 None:intergenic
TGAATAAGTTTGAAGAACTA+TGG 0.283312 5_2:-56993072 Msa0777700:CDS
TTGTTGATTCAGGGAGTAGA+AGG 0.286138 5_2:-56994488 Msa0777700:intron
ATTTCAAATGTTCGTGTCTT+AGG 0.296750 5_2:-56992565 Msa0777700:CDS
GGGAATGCTATAGTCATAAA+TGG 0.304640 5_2:-56994168 Msa0777700:CDS
AAGATGCATGTTGGGTGATA+TGG 0.328276 5_2:-56993098 Msa0777700:CDS
TCACCGTTGTTGACTCCTTT+AGG 0.330711 5_2:+56992065 None:intergenic
GTATGTGCAACATACATTAA+AGG 0.333195 5_2:+56993529 None:intergenic
CCTGTGTAAAACTAGATTCT+TGG 0.335728 5_2:+56992472 None:intergenic
ATTGCACCATACGTTTGATA+TGG 0.351243 5_2:+56993691 None:intergenic
CCAAGTTCTGAGGGACCGTT+TGG 0.359223 5_2:-56994007 Msa0777700:CDS
AACATCACCGTCAGTTATTA+CGG 0.370615 5_2:+56993240 None:intergenic
CGCAAACTAGTTTGCAATAC+GGG 0.372515 5_2:+56992779 None:intergenic
CAATATTAACATCGAGGAAA+TGG 0.372866 5_2:-56994286 Msa0777700:CDS
CCAAACGGTCCCTCAGAACT+TGG 0.376804 5_2:+56994007 None:intergenic
TTCAGACGAAGGAGATAATA+TGG 0.381516 5_2:-56994256 Msa0777700:CDS
TTGGCCTGCAAGACAACAAT+TGG 0.382041 5_2:-56993030 Msa0777700:CDS
TCCGACTATCATTCCAACTA+TGG 0.385093 5_2:-56992806 Msa0777700:CDS
CTTCCACCAGGCGTAGCAAT+TGG 0.387040 5_2:+56992146 None:intergenic
GTTTCAAAGTACTGTGGAAA+TGG 0.394957 5_2:-56992626 Msa0777700:CDS
CATTCATAGCAGTCGCGAAT+TGG 0.397674 5_2:+56993278 None:intergenic
TAACCCTCTCTCACGGTAGA+AGG 0.398623 5_2:+56995688 None:intergenic
CAATACGGGTTGACCATAGT+TGG 0.402598 5_2:+56992793 None:intergenic
CCACACCTGGTATCATTTCT+GGG 0.408982 5_2:+56993898 None:intergenic
AGCGATTCATCCGAGTCTAA+TGG 0.410491 5_2:-56994189 Msa0777700:CDS
AGAAAATCTCCCTTGTAAAC+TGG 0.412206 5_2:+56992732 None:intergenic
AGTCGTCGACGAAAGTTTAC+TGG 0.423532 5_2:-56992207 Msa0777700:CDS
TAAGGCACCTATGAAATTGC+TGG 0.427921 5_2:+56992855 None:intergenic
TCATGGTATGCAAGGGTAAA+CGG 0.428944 5_2:-56994048 Msa0777700:CDS
TATGTGCAACATACATTAAA+GGG 0.429560 5_2:+56993530 None:intergenic
AAGGATTTGTATAATCAATC+AGG 0.433589 5_2:-56993625 Msa0777700:CDS
CACATCATAGATTATGTGCT+TGG 0.435520 5_2:-56993180 Msa0777700:CDS
GAGATAATATGGATGACAAT+GGG 0.436783 5_2:-56994245 Msa0777700:CDS
GGGATTCTCAAATTGCTTCA+AGG 0.437802 5_2:-56992391 Msa0777700:CDS
CTGTGTAAAACTAGATTCTT+GGG 0.438174 5_2:+56992473 None:intergenic
CCTACTTGTGTGAGGACTAA+AGG 0.444636 5_2:-56992182 Msa0777700:CDS
TGGCCTTTGATGCAACCTAT+AGG 0.445402 5_2:-56993423 Msa0777700:CDS
AGAGACATATGTTTGGTTGT+TGG 0.446496 5_2:-56993302 Msa0777700:CDS
CTACTTGTGTGAGGACTAAA+GGG 0.449197 5_2:-56992181 Msa0777700:CDS
GCGATTCATCCGAGTCTAAT+GGG 0.449731 5_2:-56994188 Msa0777700:CDS
TGTCTTAGGATGGAGTCGTT+TGG 0.452899 5_2:-56992551 Msa0777700:CDS
TATCTTAAAAGATGCATGTT+GGG 0.464075 5_2:-56993106 Msa0777700:CDS
CAAACTGTTGTCTTTGCGAC+AGG 0.466297 5_2:-56993346 Msa0777700:CDS
CTCGAAATCGAAAATATGTA+AGG 0.466417 5_2:+56992837 None:intergenic
TATGTGCTTGGCATCTTCTA+CGG 0.467035 5_2:-56993168 Msa0777700:CDS
CACGAACATTTGAAATCTAC+AGG 0.467229 5_2:+56992572 None:intergenic
AGACGGAAGAGACATATGTT+TGG 0.472860 5_2:-56993309 Msa0777700:CDS
CGGAATGCATCATCGAATGT+TGG 0.473100 5_2:-56993148 Msa0777700:CDS
TGTAGTCAGTGTGGTGTGAT+AGG 0.474905 5_2:-56992107 Msa0777700:CDS
TATTCAGTTGTCGAAGGATG+TGG 0.485194 5_2:-56992357 Msa0777700:CDS
ATACTGTAGACAAAGATAAG+AGG 0.486855 5_2:-56993510 Msa0777700:CDS
GGAATGCATCATCGAATGTT+GGG 0.488653 5_2:-56993147 Msa0777700:CDS
CCTTTAGTCCTCACACAAGT+AGG 0.488782 5_2:+56992182 None:intergenic
TTCTCGCTTCCGCCTCTCCA+AGG 0.489867 5_2:+56993921 None:intergenic
CAGCATCGAACCAGTTTACA+AGG 0.491061 5_2:-56992742 Msa0777700:CDS
GGTGCAATGGCTAATTCATC+AGG 0.491612 5_2:-56993676 Msa0777700:CDS
TCAATCTCAATCAAATCAGT+TGG 0.493229 5_2:+56993739 None:intergenic
TTGCATATAATTTCTACTCA+TGG 0.494106 5_2:-56994065 Msa0777700:CDS
AGAGAACAGGGGATTGACCT+TGG 0.494325 5_2:-56993938 Msa0777700:CDS
TGTTGATTCAGGGAGTAGAA+GGG 0.494387 5_2:-56994487 Msa0777700:intron
GCTTCTGTTGTAGGTTATGG+AGG 0.499576 5_2:-56994340 Msa0777700:intron
CTAAATTCATCAGATATGAA+TGG 0.499723 5_2:-56994452 Msa0777700:CDS
TCGCAAACTAGTTTGCAATA+CGG 0.502777 5_2:+56992778 None:intergenic
TTGCTACGCCTGGTGGAAGT+AGG 0.503186 5_2:-56992142 Msa0777700:CDS
TTTCCTATAGGTTGCATCAA+AGG 0.505303 5_2:+56993420 None:intergenic
CACCGGAGAAAATAACAAAC+GGG 0.508832 5_2:+56993383 None:intergenic
ACGAACCACGTCTCGGTGTG+AGG 0.515344 5_2:-56992936 Msa0777700:CDS
CAAATTGCTTCAAGGTATGC+GGG 0.516691 5_2:-56992383 Msa0777700:CDS
CTGTTGGATGGTTTGCTATG+TGG 0.521362 5_2:-56993790 Msa0777700:CDS
CAAATGTTCGTGTCTTAGGA+TGG 0.523067 5_2:-56992561 Msa0777700:CDS
AATAAACGATCATGTCCTAA+AGG 0.523227 5_2:-56992080 Msa0777700:CDS
GTCGAAGGATGTGGCTGAGA+GGG 0.529678 5_2:-56992348 Msa0777700:CDS
ACACCGGAGAAAATAACAAA+CGG 0.530564 5_2:+56993382 None:intergenic
GAATAAGTTTGAAGAACTAT+GGG 0.530569 5_2:-56993071 Msa0777700:CDS
TAGACATTGTTTCAAAGCGT+TGG 0.530743 5_2:-56993849 Msa0777700:CDS
ATTTCTACTCATGGTATGCA+AGG 0.531446 5_2:-56994056 Msa0777700:CDS
CTGTCGCAAAGACAACAGTT+TGG 0.533935 5_2:+56993347 None:intergenic
TGAAGAACTATGGGAAGAGA+TGG 0.539978 5_2:-56993062 Msa0777700:CDS
CATAACCACGAGCTGTTGGA+TGG 0.541920 5_2:-56993802 Msa0777700:CDS
CGCGACTGCTATGAATGGTA+AGG 0.542367 5_2:-56993272 Msa0777700:CDS
CAAGTTCTGAGGGACCGTTT+GGG 0.542763 5_2:-56994006 Msa0777700:CDS
TGCGCATAACCACGAGCTGT+TGG 0.543217 5_2:-56993806 Msa0777700:CDS
GGTGTTAGCTTTCTATGTGC+CGG 0.543539 5_2:+56993760 None:intergenic
ACTAATCCAATTGCTACGCC+TGG 0.544424 5_2:-56992152 Msa0777700:CDS
GTTGGTATTGATGCTAGCAC+TGG 0.544748 5_2:-56992242 Msa0777700:CDS
AAGAATAAAACAACAACTGT+TGG 0.546824 5_2:-56992260 Msa0777700:CDS
GTTATGCGCAAATTCAAATA+CGG 0.547080 5_2:+56993819 None:intergenic
ATTGAAAAGTATAAGAATGT+TGG 0.559478 5_2:-56993721 Msa0777700:CDS
CGAACCACGTCTCGGTGTGA+GGG 0.561392 5_2:-56992935 Msa0777700:CDS
GCTGCAGAACATTACCCAAA+CGG 0.563552 5_2:+56993992 None:intergenic
TGAAATTCAAGAGATGTCCA+TGG 0.566346 5_2:-56992663 Msa0777700:CDS
GTTCGAGCAAGATACACAAA+TGG 0.566756 5_2:-56992425 Msa0777700:CDS
GGAGATAATATGGATGACAA+TGG 0.571879 5_2:-56994246 Msa0777700:CDS
CAATTCGCGACTGCTATGAA+TGG 0.572191 5_2:-56993277 Msa0777700:CDS
TCTGTTGTAGGTTATGGAGG+AGG 0.573234 5_2:-56994337 Msa0777700:intron
TTCGGAAGACCCAAGTTCTG+AGG 0.573488 5_2:-56994017 Msa0777700:CDS
GGCGCCTTCTACCGTGAGAG+AGG 0.573617 5_2:-56995692 Msa0777700:CDS
TCAAATTGCTTCAAGGTATG+CGG 0.574162 5_2:-56992384 Msa0777700:CDS
TTTCTGTAGATGTAGTTGAA+AGG 0.575027 5_2:-56994110 Msa0777700:CDS
AATCCAATTGCTACGCCTGG+TGG 0.577075 5_2:-56992149 Msa0777700:CDS
ATATCAAACGTATGGTGCAA+TGG 0.580935 5_2:-56993689 Msa0777700:CDS
CTTTAGTCCTCACACAAGTA+GGG 0.582163 5_2:+56992183 None:intergenic
CCACACTGACTACATGATTG+AGG 0.582317 5_2:+56992116 None:intergenic
GAGAACCCAGAAATGATACC+AGG 0.586155 5_2:-56993903 Msa0777700:CDS
GTAAGACCATATCAAACGTA+TGG 0.586746 5_2:-56993697 Msa0777700:CDS
AACTTTGTAGATAGAACCCA+TGG 0.589394 5_2:+56992646 None:intergenic
CAGGTATACGAACCACGTCT+CGG 0.590615 5_2:-56992943 Msa0777700:CDS
GGGGATTGACCTTGGAGAGG+CGG 0.591821 5_2:-56993930 Msa0777700:CDS
ATCAGATATGAATGGATGCA+TGG 0.592737 5_2:-56994444 Msa0777700:CDS
ACCATAGTTGGAATGATAGT+CGG 0.594084 5_2:+56992805 None:intergenic
GCTATAGTCATAAATGGCAT+CGG 0.596967 5_2:-56994162 Msa0777700:CDS
GTAAAACTAGATTCTTGGGA+AGG 0.598080 5_2:+56992477 None:intergenic
GGGAATTATTCAGTTGTCGA+AGG 0.603195 5_2:-56992363 Msa0777700:CDS
TGGGCGACTGCTCATATCAA+AGG 0.603639 5_2:-56992977 Msa0777700:CDS
GAAATTCAAGAGATGTCCAT+GGG 0.603913 5_2:-56992662 Msa0777700:CDS
AGAGGGTTACAAATCCCCTA+TGG 0.604842 5_2:-56995674 Msa0777700:intron
GAATGGATGCATGGATGATG+AGG 0.606403 5_2:-56994435 Msa0777700:CDS
TTGGTATTGATGCTAGCACT+GGG 0.610032 5_2:-56992241 Msa0777700:CDS
ATGCATGTTGGGTGATATGG+AGG 0.613251 5_2:-56993095 Msa0777700:CDS
TCGGAAGACCCAAGTTCTGA+GGG 0.617413 5_2:-56994016 Msa0777700:CDS
TTAGTTTCATCTGACTCTTG+CGG 0.617850 5_2:-56993589 Msa0777700:CDS
ACAGCAATAATATGATCGCA+AGG 0.619104 5_2:+56992524 None:intergenic
CAAACGGTCCCTCAGAACTT+GGG 0.622569 5_2:+56994008 None:intergenic
ATATCTTAAAAGATGCATGT+TGG 0.624704 5_2:-56993107 Msa0777700:CDS
GCAATGGCTAATTCATCAGG+TGG 0.625760 5_2:-56993673 Msa0777700:CDS
TACTGGACCCTACTTGTGTG+AGG 0.626363 5_2:-56992190 Msa0777700:CDS
TTTCTACTCATGGTATGCAA+GGG 0.627306 5_2:-56994055 Msa0777700:CDS
GCTTTGAAACAATGTCTATG+TGG 0.628276 5_2:+56993854 None:intergenic
CCTTTAACCGTAATAACTGA+CGG 0.628350 5_2:-56993247 Msa0777700:CDS
GCGCCTTCTACCGTGAGAGA+GGG 0.628935 5_2:-56995691 Msa0777700:CDS
ATGGACGAAGCTTCAGACGA+AGG 0.631163 5_2:-56994267 Msa0777700:CDS
AGGTTTAGTTACGAGAGAGA+CGG 0.633832 5_2:-56993326 Msa0777700:CDS
TACAAAGTTTCAAAGTACTG+TGG 0.634037 5_2:-56992632 Msa0777700:CDS
TTGGTTATGATGATTAACAC+CGG 0.637737 5_2:+56993366 None:intergenic
ACTGTGGAAATGGAAGTGTC+TGG 0.638067 5_2:-56992616 Msa0777700:CDS
TATAGCATTCCCATTAGACT+CGG 0.641750 5_2:+56994179 None:intergenic
GAGGGTTTACATAGTCACAT+AGG 0.642304 5_2:-56992917 Msa0777700:CDS
AATGGGGCTAATGAGTGTGA+TGG 0.645283 5_2:-56994228 Msa0777700:CDS
CCCAGAAATGATACCAGGTG+TGG 0.648094 5_2:-56993898 Msa0777700:CDS
AATCTACTTGCTCACCATAG+GGG 0.651568 5_2:+56995660 None:intergenic
TGTCGAAGGATGTGGCTGAG+AGG 0.652941 5_2:-56992349 Msa0777700:CDS
ATAATCAATCAGGAAGACAA+AGG 0.657924 5_2:-56993615 Msa0777700:CDS
GGATTTGTAACCCTCTCTCA+CGG 0.661323 5_2:+56995681 None:intergenic
TAAACCCTCACACCGAGACG+TGG 0.661419 5_2:+56992931 None:intergenic
GCAAACCATCCAACAGCTCG+TGG 0.662133 5_2:+56993797 None:intergenic
ACAGGGGATTGACCTTGGAG+AGG 0.670051 5_2:-56993933 Msa0777700:CDS
TGTGTGAGGACTAAAGGGTG+TGG 0.670824 5_2:-56992176 Msa0777700:CDS
TGAGGACGCCTACTTCCACC+AGG 0.673688 5_2:+56992134 None:intergenic
AGATAATATGGATGACAATG+GGG 0.688595 5_2:-56994244 Msa0777700:CDS
CCTCAATCATGTAGTCAGTG+TGG 0.690892 5_2:-56992116 Msa0777700:CDS
CACTAAGAAAATCTACAATG+CGG 0.712293 5_2:+56992300 None:intergenic
AGCATCGAACCAGTTTACAA+GGG 0.716912 5_2:-56992741 Msa0777700:CDS
TAATGACAATATTAACATCG+AGG 0.732342 5_2:-56994292 Msa0777700:CDS
TGTCCTAAAGGAGTCAACAA+CGG 0.735045 5_2:-56992068 Msa0777700:CDS
TGGTATTGATGCTAGCACTG+GGG 0.762460 5_2:-56992240 Msa0777700:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATTAAATAAAAGTAAAAAT+GGG + chr5_2:56992111-56992130 None:intergenic 10.0%
!! TCATTAAATAAAAGTAAAAA+TGG + chr5_2:56992112-56992131 None:intergenic 10.0%
!!! ATAAAAATAAAACTGAATTT+TGG + chr5_2:56992428-56992447 None:intergenic 10.0%
!!! TTACAGATTTTTTTTTTAAA+TGG + chr5_2:56992706-56992725 None:intergenic 10.0%
!!! TTTTTCTTAAAACTCAATTT+TGG + chr5_2:56992228-56992247 None:intergenic 15.0%
!! AATAAATTGATGCTAATTAT+TGG - chr5_2:56992820-56992839 Msa0777700:CDS 15.0%
!!! TATCTCTTTTTAATTAATGT+GGG + chr5_2:56992966-56992985 None:intergenic 15.0%
!!! TTATCTCTTTTTAATTAATG+TGG + chr5_2:56992967-56992986 None:intergenic 15.0%
!!! TTTTTTTAAATGGTGAGAAT+TGG + chr5_2:56992696-56992715 None:intergenic 20.0%
!!! TTTTTTAGAATCAATAAGCT+GGG + chr5_2:56992800-56992819 None:intergenic 20.0%
!!! TTTTTTTAGAATCAATAAGC+TGG + chr5_2:56992801-56992820 None:intergenic 20.0%
!! AATGAATGAAAAAGGTTAAT+GGG + chr5_2:56993016-56993035 None:intergenic 20.0%
!! AAATGAATGAAAAAGGTTAA+TGG + chr5_2:56993017-56993036 None:intergenic 20.0%
!! GTAAGTAAAAAGTGATTATT+AGG - chr5_2:56993291-56993310 Msa0777700:CDS 20.0%
!!! ATTGAAAAGTATAAGAATGT+TGG - chr5_2:56993980-56993999 Msa0777700:CDS 20.0%
!!! CGTATGAATTATGAAATTTT+TGG - chr5_2:56994247-56994266 Msa0777700:CDS 20.0%
!!! ATTGTATTTGTTTTTCCTAT+AGG + chr5_2:56994296-56994315 None:intergenic 20.0%
!!! TTTTGGAAATATACTCTTTA+TGG + chr5_2:56994500-56994519 None:intergenic 20.0%
! CATATTTGTTTGTAAGAAGA+TGG + chr5_2:56992285-56992304 None:intergenic 25.0%
! ACCATTATATTTGACATTGA+TGG - chr5_2:56992539-56992558 Msa0777700:CDS 25.0%
!! CCACATTTTCAATATATACA+CGG + chr5_2:56992913-56992932 None:intergenic 25.0%
! AGTAGGAAAATGAATGAAAA+AGG + chr5_2:56993024-56993043 None:intergenic 25.0%
! TCATCTATAATATCATCAGT+AGG + chr5_2:56993041-56993060 None:intergenic 25.0%
! TAATGTTGAAAATGTGTCTA+AGG + chr5_2:56993108-56993127 None:intergenic 25.0%
!!! ATCATATGTATTGTTGATTC+AGG - chr5_2:56993203-56993222 Msa0777700:CDS 25.0%
!!! TCATATGTATTGTTGATTCA+GGG - chr5_2:56993204-56993223 Msa0777700:CDS 25.0%
! CTAAATTCATCAGATATGAA+TGG - chr5_2:56993249-56993268 Msa0777700:CDS 25.0%
! TAAAATTATGCTTCTGTTGT+AGG - chr5_2:56993352-56993371 Msa0777700:CDS 25.0%
! TAATGACAATATTAACATCG+AGG - chr5_2:56993409-56993428 Msa0777700:CDS 25.0%
! TTGCATATAATTTCTACTCA+TGG - chr5_2:56993636-56993655 Msa0777700:CDS 25.0%
! AAGGATTTGTATAATCAATC+AGG - chr5_2:56994076-56994095 Msa0777700:CDS 25.0%
! TATGTGCAACATACATTAAA+GGG + chr5_2:56994174-56994193 None:intergenic 25.0%
!!! TGAAATTTTTGGTGATGTAT+TGG - chr5_2:56994258-56994277 Msa0777700:CDS 25.0%
! ATATCTTAAAAGATGCATGT+TGG - chr5_2:56994594-56994613 Msa0777700:intron 25.0%
! TATCTTAAAAGATGCATGTT+GGG - chr5_2:56994595-56994614 Msa0777700:intron 25.0%
! TGAATAAGTTTGAAGAACTA+TGG - chr5_2:56994629-56994648 Msa0777700:intron 25.0%
! GAATAAGTTTGAAGAACTAT+GGG - chr5_2:56994630-56994649 Msa0777700:intron 25.0%
! TGTATGCAAAAAAAAGAATG+TGG - chr5_2:56994704-56994723 Msa0777700:intron 25.0%
! GTATGCAAAAAAAAGAATGT+GGG - chr5_2:56994705-56994724 Msa0777700:intron 25.0%
!!! AAGATTTTTTCAGAGCATTA+CGG + chr5_2:56995002-56995021 None:intergenic 25.0%
! AAGAATAAAACAACAACTGT+TGG - chr5_2:56995441-56995460 Msa0777700:intron 25.0%
!!! TTGTTTTTGGACAAAAGGTA+TGG + chr5_2:56992172-56992191 None:intergenic 30.0%
TGTTGAATGAGAAGAAGATA+AGG + chr5_2:56992204-56992223 None:intergenic 30.0%
! ACAGAACAGATTTGTTCATA+AGG + chr5_2:56992317-56992336 None:intergenic 30.0%
AACAGAAGAATTCAGTTTGA+AGG + chr5_2:56992464-56992483 None:intergenic 30.0%
GCCATCAATGTCAAATATAA+TGG + chr5_2:56992543-56992562 None:intergenic 30.0%
CCGTGTATATATTGAAAATG+TGG - chr5_2:56992910-56992929 Msa0777700:CDS 30.0%
CAATATTAACATCGAGGAAA+TGG - chr5_2:56993415-56993434 Msa0777700:CDS 30.0%
GAGATAATATGGATGACAAT+GGG - chr5_2:56993456-56993475 Msa0777700:CDS 30.0%
AGATAATATGGATGACAATG+GGG - chr5_2:56993457-56993476 Msa0777700:CDS 30.0%
!! CATCGGTGATATTTTAAAGA+TGG - chr5_2:56993556-56993575 Msa0777700:CDS 30.0%
TTTCTGTAGATGTAGTTGAA+AGG - chr5_2:56993591-56993610 Msa0777700:CDS 30.0%
!! AGCAAACTTTTGTTTGTTCA+CGG - chr5_2:56993729-56993748 Msa0777700:CDS 30.0%
GTTATGCGCAAATTCAAATA+CGG + chr5_2:56993885-56993904 None:intergenic 30.0%
TCAATCTCAATCAAATCAGT+TGG + chr5_2:56993965-56993984 None:intergenic 30.0%
!! CAAGTTAGGTTTTGTGAAAA+AGG - chr5_2:56994057-56994076 Msa0777700:CDS 30.0%
ATAATCAATCAGGAAGACAA+AGG - chr5_2:56994086-56994105 Msa0777700:CDS 30.0%
GTATGTGCAACATACATTAA+AGG + chr5_2:56994175-56994194 None:intergenic 30.0%
ATACTGTAGACAAAGATAAG+AGG - chr5_2:56994191-56994210 Msa0777700:CDS 30.0%
TTGGTTATGATGATTAACAC+CGG + chr5_2:56994338-56994357 None:intergenic 30.0%
! CATAATCTATGATGTGCTTT+TGG + chr5_2:56994517-56994536 None:intergenic 30.0%
GAAGAGATGGTTGAAAAATT+TGG - chr5_2:56994652-56994671 Msa0777700:intron 30.0%
!!! ATCAAAGGAGAATTTTTTGC+AGG - chr5_2:56994739-56994758 Msa0777700:intron 30.0%
! ATTTTGTCCAGCAATTTCAT+AGG - chr5_2:56994839-56994858 Msa0777700:intron 30.0%
CTCGAAATCGAAAATATGTA+AGG + chr5_2:56994867-56994886 None:intergenic 30.0%
!! TACAAAGTTTCAAAGTACTG+TGG - chr5_2:56995069-56995088 Msa0777700:intron 30.0%
ATTTCAAATGTTCGTGTCTT+AGG - chr5_2:56995136-56995155 Msa0777700:intron 30.0%
!!! TATTGCTGTTTTGATGTATC+TGG - chr5_2:56995191-56995210 Msa0777700:intron 30.0%
CTGTGTAAAACTAGATTCTT+GGG + chr5_2:56995231-56995250 None:intergenic 30.0%
ACACAAATGGTTCACAATAT+TGG - chr5_2:56995289-56995308 Msa0777700:intron 30.0%
CACAAATGGTTCACAATATT+GGG - chr5_2:56995290-56995309 Msa0777700:intron 30.0%
CACTAAGAAAATCTACAATG+CGG + chr5_2:56995404-56995423 None:intergenic 30.0%
AATAAACGATCATGTCCTAA+AGG - chr5_2:56995621-56995640 Msa0777700:intron 30.0%
AAATCTACTTGCTCACCATA+GGG + chr5_2:56992045-56992064 None:intergenic 35.0%
AAAATCTACTTGCTCACCAT+AGG + chr5_2:56992046-56992065 None:intergenic 35.0%
! GTGAGCAAGTAGATTTTTAC+CGG - chr5_2:56992049-56992068 Msa0777700:CDS 35.0%
!!! GAGTGTTGTTTTTGGACAAA+AGG + chr5_2:56992177-56992196 None:intergenic 35.0%
ATCAGATATGAATGGATGCA+TGG - chr5_2:56993257-56993276 Msa0777700:CDS 35.0%
!!! AGGTATTTACTTTTGAAGCG+AGG - chr5_2:56993311-56993330 Msa0777700:CDS 35.0%
TATGCTTCTGTTGTAGGTTA+TGG - chr5_2:56993358-56993377 Msa0777700:CDS 35.0%
TTCAGACGAAGGAGATAATA+TGG - chr5_2:56993445-56993464 Msa0777700:CDS 35.0%
GGAGATAATATGGATGACAA+TGG - chr5_2:56993455-56993474 Msa0777700:CDS 35.0%
TATAGCATTCCCATTAGACT+CGG + chr5_2:56993525-56993544 None:intergenic 35.0%
GGGAATGCTATAGTCATAAA+TGG - chr5_2:56993533-56993552 Msa0777700:CDS 35.0%
GCTATAGTCATAAATGGCAT+CGG - chr5_2:56993539-56993558 Msa0777700:CDS 35.0%
ATTTCTACTCATGGTATGCA+AGG - chr5_2:56993645-56993664 Msa0777700:CDS 35.0%
TTTCTACTCATGGTATGCAA+GGG - chr5_2:56993646-56993665 Msa0777700:CDS 35.0%
!! GCAAACTTTTGTTTGTTCAC+GGG - chr5_2:56993730-56993749 Msa0777700:CDS 35.0%
!! GCTTTGAAACAATGTCTATG+TGG + chr5_2:56993850-56993869 None:intergenic 35.0%
TAGACATTGTTTCAAAGCGT+TGG - chr5_2:56993852-56993871 Msa0777700:CDS 35.0%
!! TTTGCTATGTGGTCTTTTAC+CGG - chr5_2:56993922-56993941 Msa0777700:CDS 35.0%
GTAAGACCATATCAAACGTA+TGG - chr5_2:56994004-56994023 Msa0777700:CDS 35.0%
ATTGCACCATACGTTTGATA+TGG + chr5_2:56994013-56994032 None:intergenic 35.0%
ATATCAAACGTATGGTGCAA+TGG - chr5_2:56994012-56994031 Msa0777700:CDS 35.0%
TTAGTTTCATCTGACTCTTG+CGG - chr5_2:56994112-56994131 Msa0777700:CDS 35.0%
TTTCCTATAGGTTGCATCAA+AGG + chr5_2:56994284-56994303 None:intergenic 35.0%
ACACCGGAGAAAATAACAAA+CGG + chr5_2:56994322-56994341 None:intergenic 35.0%
AGAGACATATGTTTGGTTGT+TGG - chr5_2:56994399-56994418 Msa0777700:intron 35.0%
CCTTTAACCGTAATAACTGA+CGG - chr5_2:56994454-56994473 Msa0777700:CDS 35.0%
AACATCACCGTCAGTTATTA+CGG + chr5_2:56994464-56994483 None:intergenic 35.0%
CACATCATAGATTATGTGCT+TGG - chr5_2:56994521-56994540 Msa0777700:intron 35.0%
ACCATAGTTGGAATGATAGT+CGG + chr5_2:56994899-56994918 None:intergenic 35.0%
! TCGCAAACTAGTTTGCAATA+CGG + chr5_2:56994926-56994945 None:intergenic 35.0%
AGAAAATCTCCCTTGTAAAC+TGG + chr5_2:56994972-56994991 None:intergenic 35.0%
!! TTTTCAGAGCATTACGGAAA+AGG + chr5_2:56994996-56995015 None:intergenic 35.0%
TGAAATTCAAGAGATGTCCA+TGG - chr5_2:56995038-56995057 Msa0777700:intron 35.0%
GAAATTCAAGAGATGTCCAT+GGG - chr5_2:56995039-56995058 Msa0777700:intron 35.0%
AACTTTGTAGATAGAACCCA+TGG + chr5_2:56995058-56995077 None:intergenic 35.0%
!! GTTTCAAAGTACTGTGGAAA+TGG - chr5_2:56995075-56995094 Msa0777700:intron 35.0%
! CACGAACATTTGAAATCTAC+AGG + chr5_2:56995132-56995151 None:intergenic 35.0%
ACAGCAATAATATGATCGCA+AGG + chr5_2:56995180-56995199 None:intergenic 35.0%
GTAAAACTAGATTCTTGGGA+AGG + chr5_2:56995227-56995246 None:intergenic 35.0%
CCTGTGTAAAACTAGATTCT+TGG + chr5_2:56995232-56995251 None:intergenic 35.0%
!!! CCAAGAATCTAGTTTTACAC+AGG - chr5_2:56995229-56995248 Msa0777700:intron 35.0%
!!! AGAATCTAGTTTTACACAGG+TGG - chr5_2:56995232-56995251 Msa0777700:intron 35.0%
TCAAATTGCTTCAAGGTATG+CGG - chr5_2:56995317-56995336 Msa0777700:intron 35.0%
!! TTCTTAGTGCTGAACTTTTG+AGG - chr5_2:56995415-56995434 Msa0777700:intron 35.0%
AATCTACTTGCTCACCATAG+GGG + chr5_2:56992044-56992063 None:intergenic 40.0%
!!! AAGGACGTGAGTGTTGTTTT+TGG + chr5_2:56992185-56992204 None:intergenic 40.0%
ATCCAAACCAAGCAAAGCTA+AGG - chr5_2:56992377-56992396 Msa0777700:CDS 40.0%
TCCAAACCAAGCAAAGCTAA+GGG - chr5_2:56992378-56992397 Msa0777700:CDS 40.0%
! AAGGGACTCTTTGTGTTGTT+AGG - chr5_2:56992396-56992415 Msa0777700:CDS 40.0%
! TTGATGGCCATATGTGTATG+CGG - chr5_2:56992555-56992574 Msa0777700:CDS 40.0%
! ATAAGCACCGCATACACATA+TGG + chr5_2:56992565-56992584 None:intergenic 40.0%
GTGTCTAAGGAGATCAAACT+AGG + chr5_2:56993095-56993114 None:intergenic 40.0%
!! TTGTTGATTCAGGGAGTAGA+AGG - chr5_2:56993213-56993232 Msa0777700:CDS 40.0%
!! TGTTGATTCAGGGAGTAGAA+GGG - chr5_2:56993214-56993233 Msa0777700:CDS 40.0%
TCATGGTATGCAAGGGTAAA+CGG - chr5_2:56993653-56993672 Msa0777700:CDS 40.0%
!! GGGTAAACGGATTTTCTGTT+CGG - chr5_2:56993666-56993685 Msa0777700:CDS 40.0%
!! ACTTTTGTTTGTTCACGGGA+AGG - chr5_2:56993734-56993753 Msa0777700:CDS 40.0%
!!! GGAAGGTTTTAGAGAGAACA+GGG - chr5_2:56993751-56993770 Msa0777700:CDS 40.0%
!!! GAAGGTTTTAGAGAGAACAG+GGG - chr5_2:56993752-56993771 Msa0777700:CDS 40.0%
! TCAGGTGGTTTTCACAAGTT+AGG - chr5_2:56994043-56994062 Msa0777700:CDS 40.0%
!!! AGAGGCTTGAACGTCTTTTT+TGG - chr5_2:56994209-56994228 Msa0777700:CDS 40.0%
!!! GAACGTCTTTTTTGGTGTGA+TGG - chr5_2:56994217-56994236 Msa0777700:CDS 40.0%
!!! TGCCCGTTTGTTATTTTCTC+CGG - chr5_2:56994316-56994335 Msa0777700:CDS 40.0%
CACCGGAGAAAATAACAAAC+GGG + chr5_2:56994321-56994340 None:intergenic 40.0%
AGGTTTAGTTACGAGAGAGA+CGG - chr5_2:56994375-56994394 Msa0777700:intron 40.0%
AGACGGAAGAGACATATGTT+TGG - chr5_2:56994392-56994411 Msa0777700:intron 40.0%
CCGTCAGTTATTACGGTTAA+AGG + chr5_2:56994457-56994476 None:intergenic 40.0%
TATGTGCTTGGCATCTTCTA+CGG - chr5_2:56994533-56994552 Msa0777700:intron 40.0%
GGAATGCATCATCGAATGTT+GGG - chr5_2:56994554-56994573 Msa0777700:intron 40.0%
AAGATGCATGTTGGGTGATA+TGG - chr5_2:56994603-56994622 Msa0777700:intron 40.0%
TGAAGAACTATGGGAAGAGA+TGG - chr5_2:56994639-56994658 Msa0777700:intron 40.0%
TGATCCAATTGTTGTCTTGC+AGG + chr5_2:56994678-56994697 None:intergenic 40.0%
GAGGGTTTACATAGTCACAT+AGG - chr5_2:56994784-56994803 Msa0777700:intron 40.0%
! TAAGGCACCTATGAAATTGC+TGG + chr5_2:56994849-56994868 None:intergenic 40.0%
TCCGACTATCATTCCAACTA+TGG - chr5_2:56994895-56994914 Msa0777700:intron 40.0%
! CGCAAACTAGTTTGCAATAC+GGG + chr5_2:56994925-56994944 None:intergenic 40.0%
! AGCATCGAACCAGTTTACAA+GGG - chr5_2:56994960-56994979 Msa0777700:intron 40.0%
CAAATGTTCGTGTCTTAGGA+TGG - chr5_2:56995140-56995159 Msa0777700:intron 40.0%
GTTCGAGCAAGATACACAAA+TGG - chr5_2:56995276-56995295 Msa0777700:intron 40.0%
GGGATTCTCAAATTGCTTCA+AGG - chr5_2:56995310-56995329 Msa0777700:intron 40.0%
CAAATTGCTTCAAGGTATGC+GGG - chr5_2:56995318-56995337 Msa0777700:intron 40.0%
GGGAATTATTCAGTTGTCGA+AGG - chr5_2:56995338-56995357 Msa0777700:intron 40.0%
TATTCAGTTGTCGAAGGATG+TGG - chr5_2:56995344-56995363 Msa0777700:intron 40.0%
!! TTGGTATTGATGCTAGCACT+GGG - chr5_2:56995460-56995479 Msa0777700:intron 40.0%
CTTTAGTCCTCACACAAGTA+GGG + chr5_2:56995521-56995540 None:intergenic 40.0%
CTACTTGTGTGAGGACTAAA+GGG - chr5_2:56995520-56995539 Msa0777700:intron 40.0%
TGTCCTAAAGGAGTCAACAA+CGG - chr5_2:56995633-56995652 Msa0777700:intron 40.0%
GGATTTGTAACCCTCTCTCA+CGG + chr5_2:56992023-56992042 None:intergenic 45.0%
AGAGGGTTACAAATCCCCTA+TGG - chr5_2:56992027-56992046 Msa0777700:CDS 45.0%
CCTGCTGAACTTGTAATTGC+CGG + chr5_2:56992071-56992090 None:intergenic 45.0%
!! CTTTGCTTGGTTTGGATCTC+GGG + chr5_2:56992374-56992393 None:intergenic 45.0%
!! GCTTTGCTTGGTTTGGATCT+CGG + chr5_2:56992375-56992394 None:intergenic 45.0%
!! TCCCTTAGCTTTGCTTGGTT+TGG + chr5_2:56992382-56992401 None:intergenic 45.0%
!! AAGAGTCCCTTAGCTTTGCT+TGG + chr5_2:56992387-56992406 None:intergenic 45.0%
GTGAACTGTCACATGGATGA+AGG + chr5_2:56992891-56992910 None:intergenic 45.0%
ATACACGGTGAACTGTCACA+TGG + chr5_2:56992898-56992917 None:intergenic 45.0%
CATTGAGGCAGTTGCAAATC+AGG + chr5_2:56993064-56993083 None:intergenic 45.0%
TTGCAACTGCCTCAATGTTG+TGG - chr5_2:56993067-56993086 Msa0777700:CDS 45.0%
! AACTAGGTGCCACAACATTG+AGG + chr5_2:56993079-56993098 None:intergenic 45.0%
GAATGGATGCATGGATGATG+AGG - chr5_2:56993266-56993285 Msa0777700:CDS 45.0%
GCTTCTGTTGTAGGTTATGG+AGG - chr5_2:56993361-56993380 Msa0777700:CDS 45.0%
TCTGTTGTAGGTTATGGAGG+AGG - chr5_2:56993364-56993383 Msa0777700:CDS 45.0%
AATGGGGCTAATGAGTGTGA+TGG - chr5_2:56993473-56993492 Msa0777700:CDS 45.0%
AGCGATTCATCCGAGTCTAA+TGG - chr5_2:56993512-56993531 Msa0777700:CDS 45.0%
GCGATTCATCCGAGTCTAAT+GGG - chr5_2:56993513-56993532 Msa0777700:CDS 45.0%
GCTGCAGAACATTACCCAAA+CGG + chr5_2:56993712-56993731 None:intergenic 45.0%
!!! GGGAAGGTTTTAGAGAGAAC+AGG - chr5_2:56993750-56993769 Msa0777700:CDS 45.0%
GAGAACCCAGAAATGATACC+AGG - chr5_2:56993798-56993817 Msa0777700:CDS 45.0%
CCACACCTGGTATCATTTCT+GGG + chr5_2:56993806-56993825 None:intergenic 45.0%
TCCACACCTGGTATCATTTC+TGG + chr5_2:56993807-56993826 None:intergenic 45.0%
! TTTTGCAGAACATCCACACC+TGG + chr5_2:56993819-56993838 None:intergenic 45.0%
!! CTGTTGGATGGTTTGCTATG+TGG - chr5_2:56993911-56993930 Msa0777700:CDS 45.0%
!! GGTGTTAGCTTTCTATGTGC+CGG + chr5_2:56993944-56993963 None:intergenic 45.0%
GGTGCAATGGCTAATTCATC+AGG - chr5_2:56994025-56994044 Msa0777700:CDS 45.0%
GCAATGGCTAATTCATCAGG+TGG - chr5_2:56994028-56994047 Msa0777700:CDS 45.0%
TGGCCTTTGATGCAACCTAT+AGG - chr5_2:56994278-56994297 Msa0777700:CDS 45.0%
CTGTCGCAAAGACAACAGTT+TGG + chr5_2:56994357-56994376 None:intergenic 45.0%
CAAACTGTTGTCTTTGCGAC+AGG - chr5_2:56994355-56994374 Msa0777700:intron 45.0%
CATTCATAGCAGTCGCGAAT+TGG + chr5_2:56994426-56994445 None:intergenic 45.0%
CAATTCGCGACTGCTATGAA+TGG - chr5_2:56994424-56994443 Msa0777700:intron 45.0%
CGGAATGCATCATCGAATGT+TGG - chr5_2:56994553-56994572 Msa0777700:intron 45.0%
ATGCATGTTGGGTGATATGG+AGG - chr5_2:56994606-56994625 Msa0777700:intron 45.0%
TTGGCCTGCAAGACAACAAT+TGG - chr5_2:56994671-56994690 Msa0777700:intron 45.0%
! CAATACGGGTTGACCATAGT+TGG + chr5_2:56994911-56994930 None:intergenic 45.0%
! CAGCATCGAACCAGTTTACA+AGG - chr5_2:56994959-56994978 Msa0777700:intron 45.0%
!! ACTGTGGAAATGGAAGTGTC+TGG - chr5_2:56995085-56995104 Msa0777700:intron 45.0%
TGTCTTAGGATGGAGTCGTT+TGG - chr5_2:56995150-56995169 Msa0777700:intron 45.0%
!! GTTGGTATTGATGCTAGCAC+TGG - chr5_2:56995459-56995478 Msa0777700:intron 45.0%
!! TGGTATTGATGCTAGCACTG+GGG - chr5_2:56995461-56995480 Msa0777700:intron 45.0%
AGTCGTCGACGAAAGTTTAC+TGG - chr5_2:56995494-56995513 Msa0777700:intron 45.0%
CCTTTAGTCCTCACACAAGT+AGG + chr5_2:56995522-56995541 None:intergenic 45.0%
CCTACTTGTGTGAGGACTAA+AGG - chr5_2:56995519-56995538 Msa0777700:intron 45.0%
ACTAATCCAATTGCTACGCC+TGG - chr5_2:56995549-56995568 Msa0777700:intron 45.0%
! CCACACTGACTACATGATTG+AGG + chr5_2:56995588-56995607 None:intergenic 45.0%
CCTCAATCATGTAGTCAGTG+TGG - chr5_2:56995585-56995604 Msa0777700:intron 45.0%
TGTAGTCAGTGTGGTGTGAT+AGG - chr5_2:56995594-56995613 Msa0777700:intron 45.0%
!! TCACCGTTGTTGACTCCTTT+AGG + chr5_2:56995639-56995658 None:intergenic 45.0%
TAACCCTCTCTCACGGTAGA+AGG + chr5_2:56992016-56992035 None:intergenic 50.0%
CCGGCAATTACAAGTTCAGC+AGG - chr5_2:56992068-56992087 Msa0777700:CDS 50.0%
ATGGACGAAGCTTCAGACGA+AGG - chr5_2:56993434-56993453 Msa0777700:CDS 50.0%
TTCGGAAGACCCAAGTTCTG+AGG - chr5_2:56993684-56993703 Msa0777700:CDS 50.0%
! TCGGAAGACCCAAGTTCTGA+GGG - chr5_2:56993685-56993704 Msa0777700:CDS 50.0%
CAAACGGTCCCTCAGAACTT+GGG + chr5_2:56993696-56993715 None:intergenic 50.0%
!! CAAGTTCTGAGGGACCGTTT+GGG - chr5_2:56993695-56993714 Msa0777700:CDS 50.0%
AGAGAACAGGGGATTGACCT+TGG - chr5_2:56993763-56993782 Msa0777700:CDS 50.0%
CCCAGAAATGATACCAGGTG+TGG - chr5_2:56993803-56993822 Msa0777700:CDS 50.0%
CATAACCACGAGCTGTTGGA+TGG - chr5_2:56993899-56993918 Msa0777700:CDS 50.0%
CGCGACTGCTATGAATGGTA+AGG - chr5_2:56994429-56994448 Msa0777700:intron 50.0%
TGGGCGACTGCTCATATCAA+AGG - chr5_2:56994724-56994743 Msa0777700:intron 50.0%
CAGGTATACGAACCACGTCT+CGG - chr5_2:56994758-56994777 Msa0777700:intron 50.0%
TACTGGACCCTACTTGTGTG+AGG - chr5_2:56995511-56995530 Msa0777700:intron 50.0%
TGTGTGAGGACTAAAGGGTG+TGG - chr5_2:56995525-56995544 Msa0777700:intron 50.0%
AATCCAATTGCTACGCCTGG+TGG - chr5_2:56995552-56995571 Msa0777700:intron 50.0%
CCAAACGGTCCCTCAGAACT+TGG + chr5_2:56993697-56993716 None:intergenic 55.0%
!! CCAAGTTCTGAGGGACCGTT+TGG - chr5_2:56993694-56993713 Msa0777700:CDS 55.0%
ACAGGGGATTGACCTTGGAG+AGG - chr5_2:56993768-56993787 Msa0777700:CDS 55.0%
TGCGCATAACCACGAGCTGT+TGG - chr5_2:56993895-56993914 Msa0777700:CDS 55.0%
GCAAACCATCCAACAGCTCG+TGG + chr5_2:56993907-56993926 None:intergenic 55.0%
TAAACCCTCACACCGAGACG+TGG + chr5_2:56994773-56994792 None:intergenic 55.0%
TGTCGAAGGATGTGGCTGAG+AGG - chr5_2:56995352-56995371 Msa0777700:intron 55.0%
GTCGAAGGATGTGGCTGAGA+GGG - chr5_2:56995353-56995372 Msa0777700:intron 55.0%
CTTCCACCAGGCGTAGCAAT+TGG + chr5_2:56995558-56995577 None:intergenic 55.0%
TTGCTACGCCTGGTGGAAGT+AGG - chr5_2:56995559-56995578 Msa0777700:intron 55.0%
GCGCCTTCTACCGTGAGAGA+GGG - chr5_2:56992010-56992029 Msa0777700:CDS 60.0%
GGGGATTGACCTTGGAGAGG+CGG - chr5_2:56993771-56993790 Msa0777700:CDS 60.0%
TTCTCGCTTCCGCCTCTCCA+AGG + chr5_2:56993783-56993802 None:intergenic 60.0%
! ACGAACCACGTCTCGGTGTG+AGG - chr5_2:56994765-56994784 Msa0777700:intron 60.0%
! CGAACCACGTCTCGGTGTGA+GGG - chr5_2:56994766-56994785 Msa0777700:intron 60.0%
TGAGGACGCCTACTTCCACC+AGG + chr5_2:56995570-56995589 None:intergenic 60.0%
GGCGCCTTCTACCGTGAGAG+AGG - chr5_2:56992009-56992028 Msa0777700:CDS 65.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 56992007 56995716 56992007 ID=Msa0777700;Name=Msa0777700
chr5_2 mRNA 56992007 56995716 56992007 ID=Msa0777700-mRNA-1;Parent=Msa0777700;Name=Msa0777700-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|0.5|0.33|0.66|1|1|3|0|816
chr5_2 exon 56995675 56995716 56995675 ID=Msa0777700-mRNA-1:exon:16833;Parent=Msa0777700-mRNA-1
chr5_2 exon 56994433 56994498 56994433 ID=Msa0777700-mRNA-1:exon:16832;Parent=Msa0777700-mRNA-1
chr5_2 exon 56992007 56994349 56992007 ID=Msa0777700-mRNA-1:exon:16831;Parent=Msa0777700-mRNA-1
chr5_2 CDS 56995675 56995716 56995675 ID=Msa0777700-mRNA-1:cds;Parent=Msa0777700-mRNA-1
chr5_2 CDS 56994433 56994498 56994433 ID=Msa0777700-mRNA-1:cds;Parent=Msa0777700-mRNA-1
chr5_2 CDS 56992007 56994349 56992007 ID=Msa0777700-mRNA-1:cds;Parent=Msa0777700-mRNA-1
Gene Sequence

>Msa0777700

ATGGCGCCTTCTACCGTGAGAGAGGGTTACAAATCCCCTATGGGAGTAGAAGGGAGTGATCAGTCTCTAAATTCATCAGATATGAATGGATGCATGGATGATGAGGAAGTTATGGAGGAGGTAACAGAAAGTTCAGCTGACAGTAATGACAATATTAACATCGAGGAAATGGACGAAGCTTCAGACGAAGGAGATAATATGGATGACAATGGGGCTAATGAGTGTGATGGAGATTTAGAAGCGAGTAGCGATTCATCCGAGTCTAATGGGAATGCTATAGTCATAAATGGCATCGGTGATATTTTAAAGATGGACATGAAAAACATTTCTGTAGATGTAGTTGAAAGGTTTGACTTTGCTGATTTAGATGTTGCATATAATTTCTACTCATGGTATGCAAGGGTAAACGGATTTTCTGTTCGGAAGACCCAAGTTCTGAGGGACCGTTTGGGTAATGTTCTGCAGCAAACTTTTGTTTGTTCACGGGAAGGTTTTAGAGAGAACAGGGGATTGACCTTGGAGAGGCGGAAGCGAGAACCCAGAAATGATACCAGGTGTGGATGTTCTGCAAAATGTCATGTCCACATAGACATTGTTTCAAAGCGTTGGTATGTGACCGTATTTGAATTTGCGCATAACCACGAGCTGTTGGATGGTTTGCTATGTGGTCTTTTACCGGCACATAGAAAGCTAACACCAACTGATTTGATTGAGATTGAAAAGTATAAGAATGTTGGTGTAAGACCATATCAAACGTATGGTGCAATGGCTAATTCATCAGGTGGTTTTCACAAGTTAGGTTTTGTGAAAAAGGATTTGTATAATCAATCAGGAAGACAAAGGAAATTAGTTTCATCTGACTCTTGCGGTGCACTGAAGTATCTTCATATGCTTCGTACAAAAGACCCTTTAATGTATGTTGCACATACTGTAGACAAAGATAAGAGGCTTGAACGTCTTTTTTGGTGTGATGGAGAAAGTCGTATGAATTATGAAATTTTTGGTGATGTATTGGCCTTTGATGCAACCTATAGGAAAAACAAATACAATTGCCCGTTTGTTATTTTCTCCGGTGTTAATCATCATAACCAAACTGTTGTCTTTGCGACAGGTTTAGTTACGAGAGAGACGGAAGAGACATATGTTTGGTTGTTGGACCAATTCGCGACTGCTATGAATGGTAAGGCTCCTTTAACCGTAATAACTGACGGTGATGTTGCAATGAAAAATGCCATAAAGAGTATATTTCCAAAAGCACATCATAGATTATGTGCTTGGCATCTTCTACGGAATGCATCATCGAATGTTGGGATTGCTGATTTCATGTCATATCTTAAAAGATGCATGTTGGGTGATATGGAGGTGAATAAGTTTGAAGAACTATGGGAAGAGATGGTTGAAAAATTTGGCCTGCAAGACAACAATTGGATCAATGAGATGTATGCAAAAAAAAGAATGTGGGCGACTGCTCATATCAAAGGAGAATTTTTTGCAGGTATACGAACCACGTCTCGGTGTGAGGGTTTACATAGTCACATAGGTCAATTTGTGCATTCGAGAATTAACTTGACTGATTTTGTCCAGCAATTTCATAGGTGCCTTACATATTTTCGATTTCGAGAGATTGAGTCCGACTATCATTCCAACTATGGTCAACCCGTATTGCAAACTAGTTTGCGATTCATTGAGAGATCAGCATCGAACCAGTTTACAAGGGAGATTTTCTTCCTTTTCCGTAATGCTCTGAAAAAATCTTTGATGTTGAGAATTATTGAAATTCAAGAGATGTCCATGGGTTCTATCTACAAAGTTTCAAAGTACTGTGGAAATGGAAGTGTCTGGCACGTTACACATTGTGAAACTCCTGTAGATTTCAAATGTTCGTGTCTTAGGATGGAGTCGTTTGGACTGCCTTGCGATCATATTATTGCTGTTTTGATGTATCTGGATTTTGATCACCTTCCCAAGAATCTAGTTTTACACAGGTGGACAAAGTTCGCTACAGATACAGTTCGAGCAAGATACACAAATGGTTCACAATATTGGGATTCTCAAATTGCTTCAAGGTATGCGGGAATTATTCAGTTGTCGAAGGATGTGGCTGAGAGGGTTTATCAAGATGTAAAAGAGTACAACCGCATTGTAGATTTTCTTAGTGCTGAACTTTTGAGGTTGAAGAATAAAACAACAACTGTTGGTATTGATGCTAGCACTGGGGTACACAATGTAGTCGTCGACGAAAGTTTACTGGACCCTACTTGTGTGAGGACTAAAGGGTGTGGAACTAATCCAATTGCTACGCCTGGTGGAAGTAGGCGTCCTCAATCATGTAGTCAGTGTGGTGTGATAGGACACAATAAACGATCATGTCCTAAAGGAGTCAACAACGGTGAAAATGCATGCACTTCGAGTGCGACGATTTATGAAATTAATGAAGATGATGAAGAATGA
Protein sequence

>Msa0777700

MAPSTVREGYKSPMGVEGSDQSLNSSDMNGCMDDEEVMEEVTESSADSNDNINIEEMDEASDEGDNMDDNGANECDGDLEASSDSSESNGNAIVINGIGDILKMDMKNISVDVVERFDFADLDVAYNFYSWYARVNGFSVRKTQVLRDRLGNVLQQTFVCSREGFRENRGLTLERRKREPRNDTRCGCSAKCHVHIDIVSKRWYVTVFEFAHNHELLDGLLCGLLPAHRKLTPTDLIEIEKYKNVGVRPYQTYGAMANSSGGFHKLGFVKKDLYNQSGRQRKLVSSDSCGALKYLHMLRTKDPLMYVAHTVDKDKRLERLFWCDGESRMNYEIFGDVLAFDATYRKNKYNCPFVIFSGVNHHNQTVVFATGLVTRETEETYVWLLDQFATAMNGKAPLTVITDGDVAMKNAIKSIFPKAHHRLCAWHLLRNASSNVGIADFMSYLKRCMLGDMEVNKFEELWEEMVEKFGLQDNNWINEMYAKKRMWATAHIKGEFFAGIRTTSRCEGLHSHIGQFVHSRINLTDFVQQFHRCLTYFRFREIESDYHSNYGQPVLQTSLRFIERSASNQFTREIFFLFRNALKKSLMLRIIEIQEMSMGSIYKVSKYCGNGSVWHVTHCETPVDFKCSCLRMESFGLPCDHIIAVLMYLDFDHLPKNLVLHRWTKFATDTVRARYTNGSQYWDSQIASRYAGIIQLSKDVAERVYQDVKEYNRIVDFLSAELLRLKNKTTTVGIDASTGVHNVVVDESLLDPTCVRTKGCGTNPIATPGGSRRPQSCSQCGVIGHNKRSCPKGVNNGENACTSSATIYEINEDDEE*