AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0302110

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 46 sgRNAs with CRISPR-Local

Find 287 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTAGAGGTTCAAAGAAAA+TGG 0.188117 2_3:+58727742 Msa0302110:CDS
GTTGTTGCAACTCACCTTCT+TGG 0.297659 2_3:-58727654 None:intergenic
ATTATCCCAGCTAGGAGTTT+TGG 0.335512 2_3:+58727485 Msa0302110:CDS
GAGGGGATACTTTGATGTTA+TGG 0.339007 2_3:+58724532 Msa0302110:CDS
GCTCAATCCAAGAGATTATC+AGG 0.347554 2_3:+58727623 Msa0302110:CDS
AGGCAAAATTGCACTGAGTT+GGG 0.359026 2_3:+58727562 Msa0302110:CDS
TTCAAAGGTAAAGACTTTAG+AGG 0.378092 2_3:+58727728 Msa0302110:intron
CAGGCAAAATTGCACTGAGT+TGG 0.401144 2_3:+58727561 Msa0302110:CDS
AAGAGAATCTCGACGGCGGC+GGG 0.404826 2_3:+58724177 Msa0302110:five_prime_UTR
GGATTTGCCTGATAATCTCT+TGG 0.405211 2_3:-58727630 None:intergenic
TTGCAATTGTCCCACCCCTT+TGG 0.416532 2_3:-58727062 None:intergenic
TTTAGAGGTTCAAAGAAAAT+GGG 0.440701 2_3:+58727743 Msa0302110:CDS
GAGCTGCCATCATCTTTGAC+AGG 0.454144 2_3:+58727507 Msa0302110:CDS
GTTGCAACTCACCTTCTTGG+AGG 0.459060 2_3:-58727651 None:intergenic
GTTCAAGCAACATGGTTGTA+AGG 0.459408 2_3:+58724559 Msa0302110:CDS
GCAGCTCCAAAACTCCTAGC+TGG 0.475826 2_3:-58727491 None:intergenic
GAAGAGAATCTCGACGGCGG+CGG 0.479467 2_3:+58724176 Msa0302110:five_prime_UTR
AAGTGTCAGTGCGTGCGTAA+TGG 0.481916 2_3:+58727094 Msa0302110:CDS
CAACCATGTTGTGATAAAGT+TGG 0.485000 2_3:+58727770 Msa0302110:CDS
TAATGTTAGCAGGTACCAAA+GGG 0.506277 2_3:+58727047 Msa0302110:intron
AATTGCCCTGTCAAAGATGA+TGG 0.516457 2_3:-58727513 None:intergenic
GATGCTATGATAATGTTGTC+AGG 0.516838 2_3:+58727542 Msa0302110:CDS
AAAGAAAAGAAAATCTCGGC+GGG 0.518112 2_3:+58724144 Msa0302110:five_prime_UTR
GAGTTGAAGAAGCGCGTCTG+AGG 0.522997 2_3:+58724215 Msa0302110:five_prime_UTR
GGCAAAATTGCACTGAGTTG+GGG 0.539731 2_3:+58727563 Msa0302110:CDS
CTAATGTTAGCAGGTACCAA+AGG 0.542601 2_3:+58727046 Msa0302110:intron
CAGCTCCAAAACTCCTAGCT+GGG 0.566741 2_3:-58727490 None:intergenic
CGACGGCGGCGGGGATTGTG+CGG 0.576692 2_3:+58724187 Msa0302110:five_prime_UTR
CCACAAAGAAAAGAAAATCT+CGG 0.578551 2_3:+58724140 Msa0302110:five_prime_UTR
ATCAGGCAAATCCTCCAAGA+AGG 0.580318 2_3:+58727640 Msa0302110:CDS
TCTGAGGATTGTGGTAATCA+TGG 0.586573 2_3:+58724231 Msa0302110:five_prime_UTR
AGCTGCCATCATCTTTGACA+GGG 0.613410 2_3:+58727508 Msa0302110:CDS
GTTAGCAGGTACCAAAGGGG+TGG 0.618021 2_3:+58727051 Msa0302110:intron
AGAAAAGAAAATCTCGGCGG+GGG 0.624131 2_3:+58724146 Msa0302110:five_prime_UTR
AAACCAACTTTATCACAACA+TGG 0.630612 2_3:-58727773 None:intergenic
TTAGCAGGTACCAAAGGGGT+GGG 0.638356 2_3:+58727052 Msa0302110:intron
AGAGAATCTCGACGGCGGCG+GGG 0.643308 2_3:+58724178 Msa0302110:five_prime_UTR
AGAAGCGCGTCTGAGGATTG+TGG 0.648188 2_3:+58724222 Msa0302110:five_prime_UTR
ATGGTTGAGTTCAAGCAACA+TGG 0.663784 2_3:+58724551 Msa0302110:CDS
GAACATGCATTATCCCAGCT+AGG 0.663883 2_3:+58727477 Msa0302110:CDS
TGCGTAATGGAATTTGTCAG+TGG 0.695976 2_3:+58727107 Msa0302110:CDS
GGTGGAGAAGAGAATCTCGA+CGG 0.702917 2_3:+58724170 Msa0302110:five_prime_UTR
AAGAAAAGAAAATCTCGGCG+GGG 0.715935 2_3:+58724145 Msa0302110:five_prime_UTR
CAAAGAAAAGAAAATCTCGG+CGG 0.719905 2_3:+58724143 Msa0302110:five_prime_UTR
GGAGAAGAGAATCTCGACGG+CGG 0.733930 2_3:+58724173 Msa0302110:five_prime_UTR
AATGTTAGCAGGTACCAAAG+GGG 0.753582 2_3:+58727048 Msa0302110:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTATTTATTTTTTGTTTAT+GGG + chr2_3:58724414-58724433 Msa0302110:intron 10.0%
!! TGTATATGTTATTAAAAAAA+AGG - chr2_3:58726665-58726684 None:intergenic 10.0%
!!! AAATAAATACACTTTTGAAA+AGG - chr2_3:58724405-58724424 None:intergenic 15.0%
!!! GTGTATTTATTTTTTGTTTA+TGG + chr2_3:58724413-58724432 Msa0302110:intron 15.0%
!!! TTTGAATTTTTTGATGATTA+TGG + chr2_3:58724770-58724789 Msa0302110:intron 15.0%
!!! TTATATGTTTTCTTTGATTA+TGG + chr2_3:58724928-58724947 Msa0302110:intron 15.0%
!!! CAACAAATTTTTTATATCAT+TGG - chr2_3:58724979-58724998 None:intergenic 15.0%
!! CAATGATATAAAAAATTTGT+TGG + chr2_3:58724977-58724996 Msa0302110:intron 15.0%
!!! TATAAAAAATTTGTTGGTTT+TGG + chr2_3:58724983-58725002 Msa0302110:intron 15.0%
!!! AATTCCATAACTTAATTTTT+TGG + chr2_3:58725472-58725491 Msa0302110:intron 15.0%
!! AATAAATACAAAAAAGAACT+GGG - chr2_3:58726639-58726658 None:intergenic 15.0%
!! AAATAAATACAAAAAAGAAC+TGG - chr2_3:58726640-58726659 None:intergenic 15.0%
!!! AGACATTTTTTTTTTTGATA+AGG - chr2_3:58726771-58726790 None:intergenic 15.0%
!! ATGTTTAATACAGTATTATA+TGG + chr2_3:58727368-58727387 Msa0302110:intron 15.0%
!!! TGATATCTTAATATTTTCAA+AGG + chr2_3:58727713-58727732 Msa0302110:intron 15.0%
!!! GTAATTGATATGAATTTTTC+AGG + chr2_3:58724357-58724376 Msa0302110:CDS 20.0%
!! TATTAATTTAGTGATGTTGT+TGG + chr2_3:58724490-58724509 Msa0302110:intron 20.0%
!!! TATAAACCCTAATTTTCTAT+GGG - chr2_3:58724657-58724676 None:intergenic 20.0%
!!! ATATAAACCCTAATTTTCTA+TGG - chr2_3:58724658-58724677 None:intergenic 20.0%
!!! GAATTATATGTCTAATTTGT+TGG + chr2_3:58724706-58724725 Msa0302110:intron 20.0%
!! ATAGAATCTTATGATATTCA+TGG + chr2_3:58724874-58724893 Msa0302110:intron 20.0%
!! TATCACAAAATGTGAATAAT+TGG - chr2_3:58725338-58725357 None:intergenic 20.0%
!! CTATTGCGAAAAATAATTTA+AGG + chr2_3:58725380-58725399 Msa0302110:intron 20.0%
!! ATAAATACAAAAAAGAACTG+GGG - chr2_3:58726638-58726657 None:intergenic 20.0%
!!! TTAATTGTTTTTTTTACTGC+AGG + chr2_3:58726883-58726902 Msa0302110:intron 20.0%
!!! ATTTCAAACATAAAAGCATT+TGG - chr2_3:58726963-58726982 None:intergenic 20.0%
!!! TTTTGTTACTAGAAATAGAA+AGG + chr2_3:58726988-58727007 Msa0302110:intron 20.0%
!! TTTGTTACTAGAAATAGAAA+GGG + chr2_3:58726989-58727008 Msa0302110:intron 20.0%
!!! ATACTTTTGCATAATCTATT+TGG + chr2_3:58727134-58727153 Msa0302110:intron 20.0%
!! TCAATTAGTCAATTTAGAAA+AGG - chr2_3:58727687-58727706 None:intergenic 20.0%
!!! CTTCTTCTTCAGTTTTTTTT+AGG + chr2_3:58724322-58724341 Msa0302110:CDS 25.0%
!!! TTATGGGTTTTGTTTTAGAA+AGG + chr2_3:58724430-58724449 Msa0302110:intron 25.0%
!!! TATGGGTTTTGTTTTAGAAA+GGG + chr2_3:58724431-58724450 Msa0302110:intron 25.0%
!! ATTTTTGTACAGAAATGAAG+AGG + chr2_3:58724513-58724532 Msa0302110:intron 25.0%
!! TTTTTGTACAGAAATGAAGA+GGG + chr2_3:58724514-58724533 Msa0302110:intron 25.0%
! ACAATTCCCATAGAAAATTA+GGG + chr2_3:58724648-58724667 Msa0302110:intron 25.0%
! AAATTAGGGTTTATATGAGT+TGG + chr2_3:58724662-58724681 Msa0302110:intron 25.0%
!! GTTGGATTCAATATATCTTT+TGG + chr2_3:58724680-58724699 Msa0302110:intron 25.0%
!! GATTCAATATATCTTTTGGT+TGG + chr2_3:58724684-58724703 Msa0302110:intron 25.0%
! AAAATTCAAAACCATCAATC+CGG - chr2_3:58724760-58724779 None:intergenic 25.0%
! AGAATTTAGATCATTCACTA+AGG + chr2_3:58725195-58725214 Msa0302110:intron 25.0%
! GATAAGAAAATTGTTGAAAC+TGG + chr2_3:58725292-58725311 Msa0302110:intron 25.0%
!!! TTATTTTTCGCAATAGTAAG+TGG - chr2_3:58725376-58725395 None:intergenic 25.0%
! TTGCGAAAAATAATTTAAGG+AGG + chr2_3:58725383-58725402 Msa0302110:intron 25.0%
! CAAAAAATTAAGTTATGGAG+AGG - chr2_3:58725529-58725548 None:intergenic 25.0%
!!! CTCTCCATAACTTAATTTTT+TGG + chr2_3:58725527-58725546 Msa0302110:intron 25.0%
! AGAGCCAAAAAATTAAGTTA+TGG - chr2_3:58725534-58725553 None:intergenic 25.0%
! CAGATAAATAAAAACAACAC+CGG - chr2_3:58726556-58726575 None:intergenic 25.0%
! TAAATACAAAAAAGAACTGG+GGG - chr2_3:58726637-58726656 None:intergenic 25.0%
!!! TTCTGTTCCTTTTTTATGTT+TGG + chr2_3:58726738-58726757 Msa0302110:intron 25.0%
!!! AAAAAGACAGTTTTCTAGAA+AGG - chr2_3:58726864-58726883 None:intergenic 25.0%
! AATTGAATTCTAATGTTAGC+AGG + chr2_3:58727037-58727056 Msa0302110:intron 25.0%
!!! TCAATGTGGTTATTCTTTTT+TGG + chr2_3:58727436-58727455 Msa0302110:intron 25.0%
!!! CAATGTGGTTATTCTTTTTT+GGG + chr2_3:58727437-58727456 Msa0302110:intron 25.0%
!!! TTTTCAAGTTATTGATCACT+TGG + chr2_3:58727598-58727617 Msa0302110:CDS 25.0%
! TCAATTTAGAAAAGGTTTAG+AGG - chr2_3:58727679-58727698 None:intergenic 25.0%
! TTTAGAGGTTCAAAGAAAAT+GGG + chr2_3:58727743-58727762 Msa0302110:CDS 25.0%
CCACAAAGAAAAGAAAATCT+CGG + chr2_3:58724140-58724159 Msa0302110:five_prime_UTR 30.0%
! CATCATGCATGTTAATTTTG+AGG + chr2_3:58724265-58724284 Msa0302110:exon 30.0%
AAAAAAACTGAAGAAGAAGC+TGG - chr2_3:58724321-58724340 None:intergenic 30.0%
! TTTTGTACAGAAATGAAGAG+GGG + chr2_3:58724515-58724534 Msa0302110:intron 30.0%
GACAATTCCCATAGAAAATT+AGG + chr2_3:58724647-58724666 Msa0302110:intron 30.0%
!! AATTTGTTGGCTTTCTCATT+TGG + chr2_3:58724719-58724738 Msa0302110:intron 30.0%
! TTCATGGTTCATGTGTATTT+TGG + chr2_3:58724890-58724909 Msa0302110:intron 30.0%
!!! TCATGTGTATTTTGGTGAAT+TGG + chr2_3:58724898-58724917 Msa0302110:intron 30.0%
!!! ATTTGTTGGTTTTGGTCATT+TGG + chr2_3:58724991-58725010 Msa0302110:intron 30.0%
!!! TTTGTTGGTTTTGGTCATTT+GGG + chr2_3:58724992-58725011 Msa0302110:intron 30.0%
AGATTGTACAGAATTACTGA+TGG - chr2_3:58725164-58725183 None:intergenic 30.0%
GTAATTCTGTACAATCTGAT+TGG + chr2_3:58725167-58725186 Msa0302110:intron 30.0%
CAAAATGTGAATAATTGGCA+TGG - chr2_3:58725333-58725352 None:intergenic 30.0%
GGAGCCAAAAAATTAAGTTA+TGG - chr2_3:58725479-58725498 None:intergenic 30.0%
AAAATTAAGTTATGGAGAGG+AGG - chr2_3:58725526-58725545 None:intergenic 30.0%
! CAATGGGTTTATGAAAAATC+CGG + chr2_3:58725914-58725933 Msa0302110:intron 30.0%
!! TGATTTTAATATACCAGACC+CGG - chr2_3:58725968-58725987 None:intergenic 30.0%
!! AAGTTTGGCTCTATATAACT+TGG + chr2_3:58726381-58726400 Msa0302110:intron 30.0%
ATAAATAAAAACAACACCGG+TGG - chr2_3:58726553-58726572 None:intergenic 30.0%
AAATACAAAAAAGAACTGGG+GGG - chr2_3:58726636-58726655 None:intergenic 30.0%
CAAGAGTCCAAACATAAAAA+AGG - chr2_3:58726748-58726767 None:intergenic 30.0%
!!! CACCAGATTTTTTTACAACA+GGG - chr2_3:58726929-58726948 None:intergenic 30.0%
!!! ACACCAGATTTTTTTACAAC+AGG - chr2_3:58726930-58726949 None:intergenic 30.0%
! TTCAAGAGATAATTTGTCCA+AGG + chr2_3:58727159-58727178 Msa0302110:intron 30.0%
CTGATAAAGTTAACAGCAAA+AGG - chr2_3:58727264-58727283 None:intergenic 30.0%
GACTAACTCTAATTTCAATG+TGG + chr2_3:58727422-58727441 Msa0302110:intron 30.0%
TTCAAAGGTAAAGACTTTAG+AGG + chr2_3:58727728-58727747 Msa0302110:intron 30.0%
! CTTTAGAGGTTCAAAGAAAA+TGG + chr2_3:58727742-58727761 Msa0302110:CDS 30.0%
AAACCAACTTTATCACAACA+TGG - chr2_3:58727776-58727795 None:intergenic 30.0%
! CCGAGATTTTCTTTTCTTTG+TGG - chr2_3:58724143-58724162 None:intergenic 35.0%
CAAAGAAAAGAAAATCTCGG+CGG + chr2_3:58724143-58724162 Msa0302110:five_prime_UTR 35.0%
AAAGAAAAGAAAATCTCGGC+GGG + chr2_3:58724144-58724163 Msa0302110:five_prime_UTR 35.0%
!!! TTGTTGGTTTTGGTCATTTG+GGG + chr2_3:58724993-58725012 Msa0302110:intron 35.0%
!!! TCCTTTTTTGATCGGTTTGT+TGG + chr2_3:58725839-58725858 Msa0302110:intron 35.0%
ACCAACAAACCGATCAAAAA+AGG - chr2_3:58725843-58725862 None:intergenic 35.0%
TAAACCCATTGAAACAGATC+CGG - chr2_3:58725905-58725924 None:intergenic 35.0%
TGAAAAATCCGGTTTCGATT+TGG + chr2_3:58725925-58725944 Msa0302110:intron 35.0%
GAAAAATCCGGTTTCGATTT+GGG + chr2_3:58725926-58725945 Msa0302110:intron 35.0%
TCTGTTATGTTCAACGTTGT+TGG + chr2_3:58726015-58726034 Msa0302110:intron 35.0%
TGGTCGTATTGTGTTTGTTT+CGG + chr2_3:58726054-58726073 Msa0302110:intron 35.0%
AAGCATATCTATGATCTTGG+AGG - chr2_3:58726109-58726128 None:intergenic 35.0%
ACGAAGCATATCTATGATCT+TGG - chr2_3:58726112-58726131 None:intergenic 35.0%
TCATAGATATGCTTCGTAAC+AGG + chr2_3:58726115-58726134 Msa0302110:intron 35.0%
!!! GGTTTTGAGTTGCTTTCTAT+TGG + chr2_3:58726271-58726290 Msa0302110:intron 35.0%
! GAGTTGCTTTCTATTGGATT+TGG + chr2_3:58726277-58726296 Msa0302110:intron 35.0%
CTATTGGATTTGGCTAGAAA+TGG + chr2_3:58726287-58726306 Msa0302110:intron 35.0%
TGTGGTTTCAAAATCCTCAT+CGG + chr2_3:58726405-58726424 Msa0302110:intron 35.0%
! TTTAGTTCGAAAGAGACGTT+AGG + chr2_3:58726447-58726466 Msa0302110:intron 35.0%
GATGTCTAACAGATGATGTA+TGG - chr2_3:58726531-58726550 None:intergenic 35.0%
ATTTATCTGTATCACCCGTT+TGG + chr2_3:58726567-58726586 Msa0302110:intron 35.0%
TTTATCTGTATCACCCGTTT+GGG + chr2_3:58726568-58726587 Msa0302110:intron 35.0%
GAACAAATCACATACCTTCA+TGG - chr2_3:58726840-58726859 None:intergenic 35.0%
GTCCCTGTTGTAAAAAAATC+TGG + chr2_3:58726924-58726943 Msa0302110:intron 35.0%
TAATGTTAGCAGGTACCAAA+GGG + chr2_3:58727047-58727066 Msa0302110:intron 35.0%
AGTAAATTTCAGGACTCTTG+AGG + chr2_3:58727343-58727362 Msa0302110:intron 35.0%
ATAATGCATGTTCCTGTGAT+TGG - chr2_3:58727470-58727489 None:intergenic 35.0%
! GATGCTATGATAATGTTGTC+AGG + chr2_3:58727542-58727561 Msa0302110:CDS 35.0%
CAACCATGTTGTGATAAAGT+TGG + chr2_3:58727770-58727789 Msa0302110:CDS 35.0%
AAGAAAAGAAAATCTCGGCG+GGG + chr2_3:58724145-58724164 Msa0302110:five_prime_UTR 40.0%
TCTGAGGATTGTGGTAATCA+TGG + chr2_3:58724231-58724250 Msa0302110:five_prime_UTR 40.0%
GAGGGGATACTTTGATGTTA+TGG + chr2_3:58724532-58724551 Msa0302110:CDS 40.0%
ATGGTTGAGTTCAAGCAACA+TGG + chr2_3:58724551-58724570 Msa0302110:CDS 40.0%
GTTCAAGCAACATGGTTGTA+AGG + chr2_3:58724559-58724578 Msa0302110:CDS 40.0%
! TCGAAATGCTTCAGGTTTGA+TGG + chr2_3:58725018-58725037 Msa0302110:intron 40.0%
GTTACTCTGTTGAACTCCAA+GGG - chr2_3:58725230-58725249 None:intergenic 40.0%
TGTTACTCTGTTGAACTCCA+AGG - chr2_3:58725231-58725250 None:intergenic 40.0%
CGTGGAAGAAGAAGAAAAAG+AGG - chr2_3:58725500-58725519 None:intergenic 40.0%
GAGAATCTTGTGTTGGAAGA+GGG - chr2_3:58725703-58725722 None:intergenic 40.0%
AGAGAATCTTGTGTTGGAAG+AGG - chr2_3:58725704-58725723 None:intergenic 40.0%
TCTTCCAACACAAGATTCTC+TGG + chr2_3:58725703-58725722 Msa0302110:intron 40.0%
!!! TGTGGTGGTCCTTTTTTGAT+CGG + chr2_3:58725831-58725850 Msa0302110:intron 40.0%
TCGTATTGTGTTTGTTTCGG+TGG + chr2_3:58726057-58726076 Msa0302110:intron 40.0%
TTGTGTTTGTTTCGGTGGTT+TGG + chr2_3:58726062-58726081 Msa0302110:intron 40.0%
AACAACAGACAACAAGTGCA+TGG - chr2_3:58726160-58726179 None:intergenic 40.0%
ACCAATTGAATCCAGATCGA+GGG - chr2_3:58726254-58726273 None:intergenic 40.0%
AACCAATTGAATCCAGATCG+AGG - chr2_3:58726255-58726274 None:intergenic 40.0%
TATAGAGCCAAACTTCGTGA+AGG - chr2_3:58726376-58726395 None:intergenic 40.0%
GGCTCTATATAACTTGGATG+TGG + chr2_3:58726387-58726406 Msa0302110:intron 40.0%
ATCATCTGTTAGACATCCAC+CGG + chr2_3:58726534-58726553 Msa0302110:intron 40.0%
AAAGTGTATGCCAACCCAAA+CGG - chr2_3:58726585-58726604 None:intergenic 40.0%
CTCCTGACATATACTGGAAT+CGG + chr2_3:58726689-58726708 Msa0302110:intron 40.0%
!! TACCGATTCCAGTATATGTC+AGG - chr2_3:58726694-58726713 None:intergenic 40.0%
CTAATGTTAGCAGGTACCAA+AGG + chr2_3:58727046-58727065 Msa0302110:intron 40.0%
AATGTTAGCAGGTACCAAAG+GGG + chr2_3:58727048-58727067 Msa0302110:intron 40.0%
! TGCGTAATGGAATTTGTCAG+TGG + chr2_3:58727107-58727126 Msa0302110:CDS 40.0%
! GATAATTTGTCCAAGGAGAC+TGG + chr2_3:58727166-58727185 Msa0302110:intron 40.0%
TAAATGATACCGCACTGAAG+AGG + chr2_3:58727313-58727332 Msa0302110:intron 40.0%
! ATTATCCCAGCTAGGAGTTT+TGG + chr2_3:58727485-58727504 Msa0302110:CDS 40.0%
AATTGCCCTGTCAAAGATGA+TGG - chr2_3:58727516-58727535 None:intergenic 40.0%
! CTTTGACAGGGCAATTTTGT+TGG + chr2_3:58727520-58727539 Msa0302110:CDS 40.0%
AGGCAAAATTGCACTGAGTT+GGG + chr2_3:58727562-58727581 Msa0302110:CDS 40.0%
GCTCAATCCAAGAGATTATC+AGG + chr2_3:58727623-58727642 Msa0302110:CDS 40.0%
GGATTTGCCTGATAATCTCT+TGG - chr2_3:58727633-58727652 None:intergenic 40.0%
AGAAAAGAAAATCTCGGCGG+GGG + chr2_3:58724146-58724165 Msa0302110:five_prime_UTR 45.0%
TTGGTGCATCGAAATGCTTC+CGG + chr2_3:58724738-58724757 Msa0302110:intron 45.0%
TCGAAATGCTTCCGGATTGA+TGG + chr2_3:58724746-58724765 Msa0302110:intron 45.0%
!! TTGGGGTATCGAAATGCTTC+AGG + chr2_3:58725010-58725029 Msa0302110:intron 45.0%
ACTAAGGACATAGCTACCCT+TGG + chr2_3:58725211-58725230 Msa0302110:intron 45.0%
TCCACACACCTCACATCAAA+TGG + chr2_3:58725253-58725272 Msa0302110:intron 45.0%
TCCATTTGATGTGAGGTGTG+TGG - chr2_3:58725257-58725276 None:intergenic 45.0%
CCTCACATCAAATGGAACAG+CGG + chr2_3:58725261-58725280 Msa0302110:intron 45.0%
TTCGCAATAGTAAGTGGCAG+TGG - chr2_3:58725370-58725389 None:intergenic 45.0%
!! AGCGATCTGTTTCTAGAGAG+AGG - chr2_3:58725559-58725578 None:intergenic 45.0%
!! GAGAGGTTGTTTCTAGAGAG+AGG - chr2_3:58725586-58725605 None:intergenic 45.0%
TTTGGTTGTGAGGGAAGAGA+TGG - chr2_3:58725617-58725636 None:intergenic 45.0%
! CGTGAAAGGTTTGGTTGTGA+GGG - chr2_3:58725626-58725645 None:intergenic 45.0%
! TCGTGAAAGGTTTGGTTGTG+AGG - chr2_3:58725627-58725646 None:intergenic 45.0%
GAGCTACATCGTGAAAGGTT+TGG - chr2_3:58725635-58725654 None:intergenic 45.0%
AACCTTTCACGATGTAGCTC+CGG + chr2_3:58725635-58725654 Msa0302110:intron 45.0%
TCCAACACAAGATTCTCTGG+TGG + chr2_3:58725706-58725725 Msa0302110:intron 45.0%
CCAACACAAGATTCTCTGGT+GGG + chr2_3:58725707-58725726 Msa0302110:intron 45.0%
!! ACAAGATTCTCTGGTGGGTT+TGG + chr2_3:58725712-58725731 Msa0302110:intron 45.0%
ACCACCACACAAAAATGCAC+CGG - chr2_3:58725758-58725777 None:intergenic 45.0%
GTAACACACAAACGCGTTGA+CGG - chr2_3:58725781-58725800 None:intergenic 45.0%
TGTTACACCACAAAACGCGT+CGG + chr2_3:58725795-58725814 Msa0302110:intron 45.0%
!! GTCGGTTCTAGTTTCTTGTG+TGG + chr2_3:58725813-58725832 Msa0302110:intron 45.0%
!! GGTTCTAGTTTCTTGTGTGG+TGG + chr2_3:58725816-58725835 Msa0302110:intron 45.0%
TCGTTCCGGATCTGTTTCAA+TGG + chr2_3:58725897-58725916 Msa0302110:intron 45.0%
CGTTCCGGATCTGTTTCAAT+GGG + chr2_3:58725898-58725917 Msa0302110:intron 45.0%
GAGATGACCCAAATCGAAAC+CGG - chr2_3:58725936-58725955 None:intergenic 45.0%
TGCTTCGTAACAGGTCTTCT+AGG + chr2_3:58726124-58726143 Msa0302110:intron 45.0%
CAGGTCTTCTAGGCTAGAAA+TGG + chr2_3:58726134-58726153 Msa0302110:intron 45.0%
TTGTCTGTTGTTGCTGCGAA+TGG + chr2_3:58726168-58726187 Msa0302110:intron 45.0%
TCGAGGGATGGTCAATCAAT+GGG - chr2_3:58726238-58726257 None:intergenic 45.0%
ATCGAGGGATGGTCAATCAA+TGG - chr2_3:58726239-58726258 None:intergenic 45.0%
ATTGAATCCAGATCGAGGGA+TGG - chr2_3:58726250-58726269 None:intergenic 45.0%
TCCCTCGATCTGGATTCAAT+TGG + chr2_3:58726250-58726269 Msa0302110:intron 45.0%
ATAACCCCACTATGATGCGA+CGG - chr2_3:58726325-58726344 None:intergenic 45.0%
TCTCTCACCTTCACGAAGTT+TGG + chr2_3:58726366-58726385 Msa0302110:intron 45.0%
TTGGATCCATTCCGTGTTGA+CGG + chr2_3:58726500-58726519 Msa0302110:intron 45.0%
GTATGGACAAACCGTCAACA+CGG - chr2_3:58726514-58726533 None:intergenic 45.0%
AAGTGTATGCCAACCCAAAC+GGG - chr2_3:58726584-58726603 None:intergenic 45.0%
! TACACTTTGCGTGGATTGAC+TGG + chr2_3:58726597-58726616 Msa0302110:intron 45.0%
! ACACTTTGCGTGGATTGACT+GGG + chr2_3:58726598-58726617 Msa0302110:intron 45.0%
CATGAGCTCCTGACATATAC+TGG + chr2_3:58726683-58726702 Msa0302110:intron 45.0%
! CGGTAAGACACTGCAACTTT+TGG + chr2_3:58726709-58726728 Msa0302110:intron 45.0%
ACCATTTCAAGCAGCCATGA+AGG + chr2_3:58726823-58726842 Msa0302110:intron 45.0%
ACCTTCATGGCTGCTTGAAA+TGG - chr2_3:58726827-58726846 None:intergenic 45.0%
AACACAATCACCAGTCTCCT+TGG - chr2_3:58727179-58727198 None:intergenic 45.0%
AGGCTCGAGGAGTAAATTTC+AGG + chr2_3:58727333-58727352 Msa0302110:intron 45.0%
TTGGGAACCATTCCAATCAC+AGG + chr2_3:58727455-58727474 Msa0302110:intron 45.0%
GCATGTTCCTGTGATTGGAA+TGG - chr2_3:58727465-58727484 None:intergenic 45.0%
GAACATGCATTATCCCAGCT+AGG + chr2_3:58727477-58727496 Msa0302110:CDS 45.0%
AGCTGCCATCATCTTTGACA+GGG + chr2_3:58727508-58727527 Msa0302110:CDS 45.0%
CAGGCAAAATTGCACTGAGT+TGG + chr2_3:58727561-58727580 Msa0302110:CDS 45.0%
GGCAAAATTGCACTGAGTTG+GGG + chr2_3:58727563-58727582 Msa0302110:CDS 45.0%
ATCAGGCAAATCCTCCAAGA+AGG + chr2_3:58727640-58727659 Msa0302110:CDS 45.0%
GTTGTTGCAACTCACCTTCT+TGG - chr2_3:58727657-58727676 None:intergenic 45.0%
GGTGGAGAAGAGAATCTCGA+CGG + chr2_3:58724170-58724189 Msa0302110:five_prime_UTR 50.0%
CCGCTGTTCCATTTGATGTG+AGG - chr2_3:58725264-58725283 None:intergenic 50.0%
CATCAAATGGAACAGCGGTC+AGG + chr2_3:58725266-58725285 Msa0302110:intron 50.0%
AAATGGAACAGCGGTCAGGA+AGG + chr2_3:58725270-58725289 Msa0302110:intron 50.0%
! CCACGAGAGACATTGAGTTG+TGG - chr2_3:58725443-58725462 None:intergenic 50.0%
CCACAACTCAATGTCTCTCG+TGG + chr2_3:58725440-58725459 Msa0302110:intron 50.0%
CCCACCAGAGAATCTTGTGT+TGG - chr2_3:58725710-58725729 None:intergenic 50.0%
!! AGATTCTCTGGTGGGTTTGG+TGG + chr2_3:58725715-58725734 Msa0302110:intron 50.0%
! AAAATGCACCGGCACTGCAA+TGG - chr2_3:58725747-58725766 None:intergenic 50.0%
!!! AGTGCCGGTGCATTTTTGTG+TGG + chr2_3:58725751-58725770 Msa0302110:intron 50.0%
! ACTAGAACCGACGCGTTTTG+TGG - chr2_3:58725805-58725824 None:intergenic 50.0%
GAACCGTGAACAAGCCACAA+CGG - chr2_3:58725870-58725889 None:intergenic 50.0%
!! TTGTGGCTTGTTCACGGTTC+CGG + chr2_3:58725870-58725889 Msa0302110:intron 50.0%
ACAGATCCGGAACGAAGATC+CGG - chr2_3:58725892-58725911 None:intergenic 50.0%
!! GGGTCATCTCAGTATCGATC+CGG + chr2_3:58725946-58725965 Msa0302110:intron 50.0%
!! GGTCATCTCAGTATCGATCC+GGG + chr2_3:58725947-58725966 Msa0302110:intron 50.0%
TTGACTACGTCGCGATGGTT+AGG + chr2_3:58725991-58726010 Msa0302110:intron 50.0%
!! TTGGTGTCGATGTCGTGTGT+TGG + chr2_3:58726034-58726053 Msa0302110:intron 50.0%
CTGTTGTTGCTGCGAATGGT+AGG + chr2_3:58726172-58726191 Msa0302110:intron 50.0%
TGATTGACCATCCCTCGATC+TGG + chr2_3:58726240-58726259 Msa0302110:intron 50.0%
TGATGCGACGGAAAGCTTCA+CGG - chr2_3:58726313-58726332 None:intergenic 50.0%
AGCTTTCCGTCGCATCATAG+TGG + chr2_3:58726316-58726335 Msa0302110:intron 50.0%
GCTTTCCGTCGCATCATAGT+GGG + chr2_3:58726317-58726336 Msa0302110:intron 50.0%
CTTTCCGTCGCATCATAGTG+GGG + chr2_3:58726318-58726337 Msa0302110:intron 50.0%
GTGGGGTTATGTATCCGTGT+TGG + chr2_3:58726335-58726354 Msa0302110:intron 50.0%
GGGTTATGTATCCGTGTTGG+CGG + chr2_3:58726338-58726357 Msa0302110:intron 50.0%
GAGAGATTCTACCGCCAACA+CGG - chr2_3:58726352-58726371 None:intergenic 50.0%
CAACATCAGACAGACCGATG+AGG - chr2_3:58726422-58726441 None:intergenic 50.0%
CTCATCGGTCTGTCTGATGT+TGG + chr2_3:58726420-58726439 Msa0302110:intron 50.0%
!! TTAGGCTTGTTGCTGTCGCA+AGG + chr2_3:58726465-58726484 Msa0302110:intron 50.0%
TTGCTGTCGCAAGGAGTTGT+TGG + chr2_3:58726474-58726493 Msa0302110:intron 50.0%
GTCAACACGGAATGGATCCA+AGG - chr2_3:58726501-58726520 None:intergenic 50.0%
GACAAACCGTCAACACGGAA+TGG - chr2_3:58726509-58726528 None:intergenic 50.0%
TCTGTATCACCCGTTTGGGT+TGG + chr2_3:58726572-58726591 Msa0302110:intron 50.0%
! TTGCGTGGATTGACTGGGTT+AGG + chr2_3:58726603-58726622 Msa0302110:intron 50.0%
TTAGCAGGTACCAAAGGGGT+GGG + chr2_3:58727052-58727071 Msa0302110:intron 50.0%
TTGCAATTGTCCCACCCCTT+TGG - chr2_3:58727065-58727084 None:intergenic 50.0%
AAGTGTCAGTGCGTGCGTAA+TGG + chr2_3:58727094-58727113 Msa0302110:CDS 50.0%
CAGCTCCAAAACTCCTAGCT+GGG - chr2_3:58727493-58727512 None:intergenic 50.0%
GAGCTGCCATCATCTTTGAC+AGG + chr2_3:58727507-58727526 Msa0302110:CDS 50.0%
GTTGCAACTCACCTTCTTGG+AGG - chr2_3:58727654-58727673 None:intergenic 50.0%
AAAGAAAATCTCGGCGGGGG+TGG + chr2_3:58724149-58724168 Msa0302110:five_prime_UTR 55.0%
GGAGAAGAGAATCTCGACGG+CGG + chr2_3:58724173-58724192 Msa0302110:five_prime_UTR 55.0%
! GAGTTGAAGAAGCGCGTCTG+AGG + chr2_3:58724215-58724234 Msa0302110:five_prime_UTR 55.0%
! AGAAGCGCGTCTGAGGATTG+TGG + chr2_3:58724222-58724241 Msa0302110:five_prime_UTR 55.0%
GTTATGGAGAGGAGGTCACG+TGG - chr2_3:58725518-58725537 None:intergenic 55.0%
GGTTGTGAGGGAAGAGATGG+CGG - chr2_3:58725614-58725633 None:intergenic 55.0%
!! TTCTCTGGTGGGTTTGGTGG+CGG + chr2_3:58725718-58725737 Msa0302110:intron 55.0%
! GCCGGTGCATTTTTGTGTGG+TGG + chr2_3:58725754-58725773 Msa0302110:intron 55.0%
!! GTTTGTTGGTTCCGCCGTTG+TGG + chr2_3:58725853-58725872 Msa0302110:intron 55.0%
ACGGTTCCGGATCTTCGTTC+CGG + chr2_3:58725883-58725902 Msa0302110:intron 55.0%
!! TCTCAGTATCGATCCGGGTC+TGG + chr2_3:58725952-58725971 Msa0302110:intron 55.0%
! CACTGTTGACTACGTCGCGA+TGG + chr2_3:58725986-58726005 Msa0302110:intron 55.0%
GAGGAGCAGTACAACGCTGA+GGG - chr2_3:58726090-58726109 None:intergenic 55.0%
GTTGCTGCGAATGGTAGGTG+CGG + chr2_3:58726177-58726196 Msa0302110:intron 55.0%
TTGCTGCGAATGGTAGGTGC+GGG + chr2_3:58726178-58726197 Msa0302110:intron 55.0%
CGCAAGGAGTTGTTGGTCCT+TGG + chr2_3:58726481-58726500 Msa0302110:intron 55.0%
! GGGTTGGCATACACTTTGCG+TGG + chr2_3:58726588-58726607 Msa0302110:intron 55.0%
GTTAGCAGGTACCAAAGGGG+TGG + chr2_3:58727051-58727070 Msa0302110:intron 55.0%
GCAGCTCCAAAACTCCTAGC+TGG - chr2_3:58727494-58727513 None:intergenic 55.0%
GAAGAGAATCTCGACGGCGG+CGG + chr2_3:58724176-58724195 Msa0302110:five_prime_UTR 60.0%
AAGAGAATCTCGACGGCGGC+GGG + chr2_3:58724177-58724196 Msa0302110:five_prime_UTR 60.0%
!! AAGAGATGGCGGTGGTGGAG+AGG - chr2_3:58725603-58725622 None:intergenic 60.0%
! TGTGAGGGAAGAGATGGCGG+TGG - chr2_3:58725611-58725630 None:intergenic 60.0%
CGCCGGAGCTACATCGTGAA+AGG - chr2_3:58725640-58725659 None:intergenic 60.0%
CCGTGAACAAGCCACAACGG+CGG - chr2_3:58725867-58725886 None:intergenic 60.0%
!! CCGCCGTTGTGGCTTGTTCA+CGG + chr2_3:58725864-58725883 Msa0302110:intron 60.0%
GGAGGAGCAGTACAACGCTG+AGG - chr2_3:58726091-58726110 None:intergenic 60.0%
AGGTGCGGGTGTTTCCGTGT+TGG + chr2_3:58726192-58726211 Msa0302110:intron 60.0%
TGCGGGTGTTTCCGTGTTGG+CGG + chr2_3:58726195-58726214 Msa0302110:intron 60.0%
TACCGCACTGAAGAGGCTCG+AGG + chr2_3:58727320-58727339 Msa0302110:intron 60.0%
CTCCTCGAGCCTCTTCAGTG+CGG - chr2_3:58727325-58727344 None:intergenic 60.0%
GAAAATCTCGGCGGGGGTGG+TGG + chr2_3:58724152-58724171 Msa0302110:five_prime_UTR 65.0%
AGAGAATCTCGACGGCGGCG+GGG + chr2_3:58724178-58724197 Msa0302110:five_prime_UTR 65.0%
! GAGGGAAGAGATGGCGGTGG+TGG - chr2_3:58725608-58725627 None:intergenic 65.0%
ACGATGTAGCTCCGGCGTCG+AGG + chr2_3:58725643-58725662 Msa0302110:intron 65.0%
!! TCTGGTGGGTTTGGTGGCGG+TGG + chr2_3:58725721-58725740 Msa0302110:intron 65.0%
! TTCCGTGTTGGCGGTGCTCG+TGG + chr2_3:58726204-58726223 Msa0302110:intron 65.0%
! CACCACGAGCACCGCCAACA+CGG - chr2_3:58726209-58726228 None:intergenic 65.0%
CGATCGCGCTACCTCGACGC+CGG - chr2_3:58725657-58725676 None:intergenic 70.0%
GGCGGTGGCCATTGCAGTGC+CGG + chr2_3:58725736-58725755 Msa0302110:intron 70.0%
CGACGGCGGCGGGGATTGTG+CGG + chr2_3:58724187-58724206 Msa0302110:five_prime_UTR 75.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 58724125 58727797 58724125 ID=Msa0302110;Name=Msa0302110
chr2_3 mRNA 58724125 58727797 58724125 ID=Msa0302110-mRNA-1;Parent=Msa0302110;Name=Msa0302110-mRNA-1;_AED=0.48;_eAED=0.48;_QI=148|0.25|0.4|0.6|0.5|0.6|5|0|159
chr2_3 exon 58724125 58724378 58724125 ID=Msa0302110-mRNA-1:exon:18972;Parent=Msa0302110-mRNA-1
chr2_3 exon 58724525 58724580 58724525 ID=Msa0302110-mRNA-1:exon:18973;Parent=Msa0302110-mRNA-1
chr2_3 exon 58727059 58727128 58727059 ID=Msa0302110-mRNA-1:exon:18974;Parent=Msa0302110-mRNA-1
chr2_3 exon 58727477 58727661 58727477 ID=Msa0302110-mRNA-1:exon:18975;Parent=Msa0302110-mRNA-1
chr2_3 exon 58727735 58727797 58727735 ID=Msa0302110-mRNA-1:exon:18976;Parent=Msa0302110-mRNA-1
chr2_3 five_prime_UTR 58724125 58724272 58724125 ID=Msa0302110-mRNA-1:five_prime_utr;Parent=Msa0302110-mRNA-1
chr2_3 CDS 58724273 58724378 58724273 ID=Msa0302110-mRNA-1:cds;Parent=Msa0302110-mRNA-1
chr2_3 CDS 58724525 58724580 58724525 ID=Msa0302110-mRNA-1:cds;Parent=Msa0302110-mRNA-1
chr2_3 CDS 58727059 58727128 58727059 ID=Msa0302110-mRNA-1:cds;Parent=Msa0302110-mRNA-1
chr2_3 CDS 58727477 58727661 58727477 ID=Msa0302110-mRNA-1:cds;Parent=Msa0302110-mRNA-1
chr2_3 CDS 58727735 58727797 58727735 ID=Msa0302110-mRNA-1:cds;Parent=Msa0302110-mRNA-1
Gene Sequence

>Msa0302110

ATGTTAATTTTGAGGTTACTGTTTCACGACGTTCGTCTTCTTCGTCCAGCTTCTTCTTCAGTTTTTTTTAGGTTTCTCTCTAAAGTAATTGATATGAATTTTTCAGAAATGAAGAGGGGATACTTTGATGTTATGGTTGAGTTCAAGCAACATGGTTGTAAGGTACCAAAGGGGTGGGACAATTGCAAAGTTAAGCAAAGTGTCAGTGCGTGCGTAATGGAATTTGTCAGTGGAACATGCATTATCCCAGCTAGGAGTTTTGGAGCTGCCATCATCTTTGACAGGGCAATTTTGTTGGATGCTATGATAATGTTGTCAGGCAAAATTGCACTGAGTTGGGGCAAACTGCATTTTTTTCAAGTTATTGATCACTTGGCTGCTCAATCCAAGAGATTATCAGGCAAATCCTCCAAGAAGGTAAAGACTTTAGAGGTTCAAAGAAAATGGGAAGACAACCATGTTGTGATAAAGTTGGTTTGA
Protein sequence

>Msa0302110

MLILRLLFHDVRLLRPASSSVFFRFLSKVIDMNFSEMKRGYFDVMVEFKQHGCKVPKGWDNCKVKQSVSACVMEFVSGTCIIPARSFGAAIIFDRAILLDAMIMLSGKIALSWGKLHFFQVIDHLAAQSKRLSGKSSKKVKTLEVQRKWEDNHVVIKLV*