AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0302440

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0302440 MtrunA17_Chr2g0328161 100.000 253 0 0 1 253 1 253 0.0 513
Msa0302440 MtrunA17_Chr7g0264951 63.200 250 89 2 4 252 62 309 3.99e-110 320
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0302440 AT3G55440.1 81.423 253 47 0 1 253 1 253 4.40e-154 429
Msa0302440 AT2G21170.3 63.600 250 88 2 4 252 65 312 1.79e-109 318
Msa0302440 AT2G21170.1 63.600 250 88 2 4 252 65 312 1.79e-109 318
Msa0302440 AT2G21170.2 62.083 240 88 2 14 252 66 303 6.90e-100 293

Find 119 sgRNAs with CRISPR-Local

Find 217 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAGGTCCATGCTGCTTTA+AGG 0.106869 2_3:-59222720 Msa0302440:intron
TGCTGAAATGCTTGTTAATT+TGG 0.126755 2_3:-59223510 Msa0302440:CDS
TACTACATGCTAGCTCTTTA+TGG 0.167583 2_3:+59221973 None:intergenic
CTTATACATTACGAGGTTTC+AGG 0.215165 2_3:-59221786 Msa0302440:three_prime_UTR
TTATTATAAACAAATATAAT+AGG 0.225661 2_3:-59221897 Msa0302440:three_prime_UTR
GATATTCCTTGGGTTATTCT+TGG 0.231384 2_3:-59223488 Msa0302440:CDS
TTGCATTATAAGAGCCTATT+AGG 0.248380 2_3:-59225189 Msa0302440:five_prime_UTR
GCTTAAAGCATATTCTTCTT+TGG 0.258020 2_3:-59225218 Msa0302440:five_prime_UTR
ATAAGTGTATTGAATCAATT+TGG 0.259889 2_3:-59222074 Msa0302440:three_prime_UTR
TGTGTTATGTCTTGAGGATT+TGG 0.279401 2_3:-59222214 Msa0302440:three_prime_UTR
AAACTGGGATAATGTTGTTT+TGG 0.298614 2_3:-59222974 Msa0302440:CDS
TCTCTGCTGAACTATGTTCT+AGG 0.305712 2_3:-59222114 Msa0302440:three_prime_UTR
ACGTTTATCGCCATTGTCTT+AGG 0.313974 2_3:-59222263 Msa0302440:intron
TCCATGCTGCTTTAAGGAAA+TGG 0.316327 2_3:-59222714 Msa0302440:CDS
CTCATCGGTGGGCGATGAAT+TGG 0.317824 2_3:-59222373 Msa0302440:three_prime_UTR
AGGCTGAGATGCCAATTCTT+TGG 0.323629 2_3:+59222615 None:intergenic
AATTCTTCGTCGGTGGTAAC+TGG 0.328227 2_3:-59225056 Msa0302440:CDS
CTGTCCTGTCCTGTCCTAAT+AGG 0.332728 2_3:+59225175 None:intergenic
TATTATAAACAAATATAATA+GGG 0.346860 2_3:-59221896 Msa0302440:three_prime_UTR
TGTTAATTTGGATATTCCTT+GGG 0.361669 2_3:-59223498 Msa0302440:CDS
GCATCTCAGCCTGATATTGA+TGG 0.369973 2_3:-59222604 Msa0302440:CDS
TTGCAGACAAAGTATCAAAC+TGG 0.370984 2_3:-59222990 Msa0302440:intron
TTGTTAATTTGGATATTCCT+TGG 0.377981 2_3:-59223499 Msa0302440:CDS
GGAAAGTCATTGCTTGCATT+GGG 0.383771 2_3:-59223250 Msa0302440:CDS
TTTGGCATATGAGCCAGTTT+GGG 0.383886 2_3:-59222956 Msa0302440:CDS
GCCTCTGTAAGGATTATCTA+TGG 0.388025 2_3:-59222661 Msa0302440:CDS
CCAGTTTGGGCCATTGGGAC+TGG 0.396885 2_3:-59222943 Msa0302440:CDS
CGGAGGAAACTCCAAAGAAT+TGG 0.404230 2_3:-59222626 Msa0302440:CDS
CAAAGGGAAGGAACACATAA+GGG 0.405048 2_3:+59223844 None:intergenic
TATGAGCCAGTTTGGGCCAT+TGG 0.406432 2_3:-59222949 Msa0302440:CDS
TGATGACGACAATTCACTTC+CGG 0.406771 2_3:+59222147 None:intergenic
GTCAATGAACTCTGGCTTCA+AGG 0.409151 2_3:+59222561 None:intergenic
CGAGGTTTCAGGTTTAAACT+TGG 0.409231 2_3:-59221775 Msa0302440:three_prime_UTR
TCTAGAACAACAAGAAGAAA+AGG 0.412429 2_3:-59225129 Msa0302440:five_prime_UTR
GTCGTGACTGCCATAACATT+TGG 0.413861 2_3:-59222325 Msa0302440:three_prime_UTR
GTGCTTACACCGGGGAGATT+AGG 0.414986 2_3:-59223758 Msa0302440:intron
ATCTATGGAGGTTCTGTAAA+CGG 0.416225 2_3:-59222646 Msa0302440:CDS
ATGAGCCAGTTTGGGCCATT+GGG 0.421695 2_3:-59222948 Msa0302440:CDS
TTGAACGAGGCTCAAGTTCC+CGG 0.427132 2_3:-59224454 Msa0302440:CDS
TTATTACTAACCAAATGTTA+TGG 0.428730 2_3:+59222315 None:intergenic
TTAAGTAAAACACGGGCGTT+CGG 0.431142 2_3:+59222290 None:intergenic
CCAGTCCCAATGGCCCAAAC+TGG 0.435261 2_3:+59222943 None:intergenic
CCATGCTGCTTTAAGGAAAT+GGG 0.440149 2_3:-59222713 Msa0302440:CDS
AAAGGTTGCTTCTCCTGCTC+AGG 0.446019 2_3:-59222920 Msa0302440:CDS
TGCTGCTTTCCAAGCTGCTT+CGG 0.446387 2_3:-59222483 Msa0302440:three_prime_UTR
GGCGACAGACCGGTTGGTTA+CGG 0.448337 2_3:-59222053 Msa0302440:three_prime_UTR
GAGCAAACAAAGGCAATTGC+AGG 0.449292 2_3:-59223179 Msa0302440:intron
AGGAAAGTCATTGCTTGCAT+TGG 0.454029 2_3:-59223251 Msa0302440:CDS
TGTTGCTGCTCAAAACGCTT+GGG 0.460320 2_3:-59223793 Msa0302440:CDS
CAGAAATTTCCGAAGCAGCT+TGG 0.467398 2_3:+59222474 None:intergenic
CTGAAGTTGCTGCCTCTGTA+AGG 0.468253 2_3:-59222672 Msa0302440:CDS
TATCATCCGCTACAGTTTGT+GGG 0.468679 2_3:-59223305 Msa0302440:intron
ATCGCCCACCGATGAGGTAA+AGG 0.469952 2_3:+59222380 None:intergenic
CTCACCAACAACATCTTCTC+CGG 0.472241 2_3:+59224435 None:intergenic
GCAAAGGTGGTGCTTACACC+GGG 0.472783 2_3:-59223767 Msa0302440:CDS
AACAAATATAATAGGGGAGT+GGG 0.482103 2_3:-59221889 Msa0302440:three_prime_UTR
ATGTTGCTGCTCAAAACGCT+TGG 0.482349 2_3:-59223794 Msa0302440:CDS
CAGGCTGAACCCATTGATCA+AGG 0.495327 2_3:-59222029 Msa0302440:three_prime_UTR
GTTGCATATGCACTCTCACT+AGG 0.495343 2_3:-59223275 Msa0302440:CDS
CGCAAAGGTGGTGCTTACAC+CGG 0.498016 2_3:-59223768 Msa0302440:CDS
CTAGAACAACAAGAAGAAAA+GGG 0.510864 2_3:-59225128 Msa0302440:five_prime_UTR
TCAGCCTGTCCGTAACCAAC+CGG 0.512292 2_3:+59222044 None:intergenic
TTGGATTGTGTTATGTCTTG+AGG 0.512893 2_3:-59222220 Msa0302440:intron
TCGCCCACCGATGAGGTAAA+GGG 0.517509 2_3:+59222381 None:intergenic
AAACAAATATAATAGGGGAG+TGG 0.521187 2_3:-59221890 Msa0302440:three_prime_UTR
AACGCTTGGGTTCGCAAAGG+TGG 0.525911 2_3:-59223780 Msa0302440:CDS
ACTACCTCTTGAGCCTGAGC+AGG 0.528540 2_3:+59222907 None:intergenic
ACTCTTGAACAACGTGAAGC+TGG 0.531573 2_3:-59223224 Msa0302440:CDS
ACAAAGGGAAGGAACACATA+AGG 0.532183 2_3:+59223843 None:intergenic
TTCTCCTGCTCAGGCTCAAG+AGG 0.539203 2_3:-59222911 Msa0302440:intron
GTTCCCGGAGAAGATGTTGT+TGG 0.540063 2_3:-59224439 Msa0302440:intron
CTCTGCTGAACTATGTTCTA+GGG 0.543887 2_3:-59222113 Msa0302440:three_prime_UTR
TAAACACAAGTTCAAAGCAT+TGG 0.547440 2_3:+59222424 None:intergenic
GAGGTTTCAGGTTTAAACTT+GGG 0.552233 2_3:-59221774 Msa0302440:three_prime_UTR
ATTATAAACAAATATAATAG+GGG 0.554042 2_3:-59221895 Msa0302440:three_prime_UTR
TTGGGCCATTGGGACTGGAA+AGG 0.554351 2_3:-59222938 Msa0302440:CDS
CCCATTTCCTTAAAGCAGCA+TGG 0.555610 2_3:+59222713 None:intergenic
GAATCAATTTGGCGACAGAC+CGG 0.559054 2_3:-59222063 Msa0302440:three_prime_UTR
CAATTTGGCGACAGACCGGT+TGG 0.561018 2_3:-59222059 Msa0302440:three_prime_UTR
TCACCAACAACATCTTCTCC+GGG 0.561852 2_3:+59224436 None:intergenic
TTCTTGGCCACTCTGAGCGA+AGG 0.565910 2_3:-59223472 Msa0302440:CDS
TTATAAGAGCCTATTAGGAC+AGG 0.566780 2_3:-59225184 Msa0302440:five_prime_UTR
TTTCCCTTTACCTCATCGGT+GGG 0.569814 2_3:-59222384 Msa0302440:three_prime_UTR
TCGTAATGTATAAGTTGAAG+TGG 0.578365 2_3:+59221795 None:intergenic
TTGATGATGTCAATGAACTC+TGG 0.578436 2_3:+59222553 None:intergenic
GCTTCTAAATGAAACAAATG+AGG 0.602961 2_3:-59223447 Msa0302440:intron
ACTTCAACTTATACATTACG+AGG 0.604440 2_3:-59221793 Msa0302440:three_prime_UTR
TATCATCAGACCTAAGACAA+TGG 0.607431 2_3:+59222253 None:intergenic
AGAGCCTATTAGGACAGGAC+AGG 0.608412 2_3:-59225179 Msa0302440:five_prime_UTR
GAAGAGGTGAAGAAAATCGT+TGG 0.610298 2_3:-59224481 Msa0302440:CDS
AGCAACCTTTCCAGTCCCAA+TGG 0.611585 2_3:+59222933 None:intergenic
CAATTCATCGCCCACCGATG+AGG 0.616929 2_3:+59222374 None:intergenic
ATGGGCAGAAAATTCTTCGT+CGG 0.618039 2_3:-59225066 Msa0302440:CDS
TTGCATATGCACTCTCACTA+GGG 0.619513 2_3:-59223274 Msa0302440:CDS
GAATTGTCGTCATCACAGAA+AGG 0.621483 2_3:-59222139 Msa0302440:three_prime_UTR
AAAGGGAAGGAACACATAAG+GGG 0.623658 2_3:+59223845 None:intergenic
GGCAGAAAATTCTTCGTCGG+TGG 0.629014 2_3:-59225063 Msa0302440:CDS
GAAAGTCATTGCTTGCATTG+GGG 0.633688 2_3:-59223249 Msa0302440:CDS
AGGCTGAACCCATTGATCAA+GGG 0.634403 2_3:-59222028 Msa0302440:three_prime_UTR
AAGGGAAGGAACACATAAGG+GGG 0.637952 2_3:+59223846 None:intergenic
TGCAGACAAAGTATCAAACT+GGG 0.639857 2_3:-59222989 Msa0302440:intron
CAGACCGGTTGGTTACGGAC+AGG 0.641537 2_3:-59222048 Msa0302440:three_prime_UTR
CAAAACGCTTGGGTTCGCAA+AGG 0.648775 2_3:-59223783 Msa0302440:CDS
TGTTGTTGCTGAGCAAACAA+AGG 0.653424 2_3:-59223189 Msa0302440:CDS
TCTGTAAGGATTATCTATGG+AGG 0.664957 2_3:-59222658 Msa0302440:CDS
ACGTACAGTTAAGTAAAACA+CGG 0.667629 2_3:+59222282 None:intergenic
TCCATAGATAATCCTTACAG+AGG 0.672902 2_3:+59222660 None:intergenic
GCAGAATGGAACCACTGAAG+AGG 0.675685 2_3:-59224497 Msa0302440:intron
CATATGCACTCTCACTAGGG+AGG 0.678402 2_3:-59223271 Msa0302440:CDS
GAAGCTGCCTTCGCTCAGAG+TGG 0.679228 2_3:+59223465 None:intergenic
GAGTGGCCAAGAATAACCCA+AGG 0.679606 2_3:+59223482 None:intergenic
CGTACAGTTAAGTAAAACAC+GGG 0.684223 2_3:+59222283 None:intergenic
GATAACATACCTAATCTCCC+CGG 0.689404 2_3:+59223749 None:intergenic
TATGGAGGTTCTGTAAACGG+AGG 0.700353 2_3:-59222643 Msa0302440:CDS
AATCGTTGGTACTTTGAACG+AGG 0.707863 2_3:-59224467 Msa0302440:CDS
AAATGGGTTCACGACAACGT+AGG 0.725939 2_3:-59222697 Msa0302440:CDS
ACTTCAGTTCTTCTTCACTG+TGG 0.744514 2_3:+59222525 None:intergenic
AGGGAAGGAACACATAAGGG+GGG 0.750761 2_3:+59223847 None:intergenic
CAAAGGTGGTGCTTACACCG+GGG 0.762885 2_3:-59223766 Msa0302440:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATATTTAGTTAAAAATTTA+AGG + chr2_3:59222952-59222971 None:intergenic 10.0%
!!! TCAATAAAAATAATTTTTGT+TGG + chr2_3:59223079-59223098 None:intergenic 10.0%
!!! AAATTTTAAAAACAGTATAT+AGG + chr2_3:59224144-59224163 None:intergenic 10.0%
!! ATTATAAACAAATATAATAG+GGG - chr2_3:59225126-59225145 Msa0302440:five_prime_UTR 10.0%
!! TGATGAAATAAATCAAATAA+TGG + chr2_3:59223964-59223983 None:intergenic 15.0%
!! GTTTCAGAATTAAGATTTAT+AGG + chr2_3:59222107-59222126 None:intergenic 20.0%
!! AACTATGTTAAAAATCAGAT+CGG - chr2_3:59222124-59222143 Msa0302440:three_prime_UTR 20.0%
!!! TGATTTTCTCAAAATATTAC+CGG + chr2_3:59222254-59222273 None:intergenic 20.0%
!! GTATATAGACTATATATTTC+TGG - chr2_3:59222472-59222491 Msa0302440:three_prime_UTR 20.0%
!!! AGTAAATTTCAGTATTGATT+TGG - chr2_3:59222696-59222715 Msa0302440:CDS 20.0%
!!! ATAAGAAATTTTTTTCCAAG+AGG - chr2_3:59222764-59222783 Msa0302440:intron 20.0%
!!! TAAGAAATTTTTTTCCAAGA+GGG - chr2_3:59222765-59222784 Msa0302440:intron 20.0%
!!! TCTCATTTTAATAATGCAAA+GGG + chr2_3:59222818-59222837 None:intergenic 20.0%
!! TTAACTAAATATCAAGTTTC+TGG - chr2_3:59222959-59222978 Msa0302440:CDS 20.0%
!! TACAGAGATACAAAAAAAAA+AGG + chr2_3:59223029-59223048 None:intergenic 20.0%
!! TTATTACTAACCAAATGTTA+TGG + chr2_3:59224709-59224728 None:intergenic 20.0%
!! ATAAGTGTATTGAATCAATT+TGG - chr2_3:59224947-59224966 Msa0302440:intron 20.0%
! AATCATCGAAATCAAAAACA+TGG - chr2_3:59221936-59221955 Msa0302440:three_prime_UTR 25.0%
! ATCATCGAAATCAAAAACAT+GGG - chr2_3:59221937-59221956 Msa0302440:three_prime_UTR 25.0%
! CAACTAAAAACTAACTAGAT+CGG + chr2_3:59222209-59222228 None:intergenic 25.0%
!!! ATATTTCTGGTGATTCATAT+TGG - chr2_3:59222485-59222504 Msa0302440:three_prime_UTR 25.0%
!!! GTCTCATTTTAATAATGCAA+AGG + chr2_3:59222819-59222838 None:intergenic 25.0%
! GAGACTTAAATTCATTATAC+AGG - chr2_3:59222834-59222853 Msa0302440:intron 25.0%
!! ACGAAATTTGCTTATCTATT+TGG - chr2_3:59222925-59222944 Msa0302440:CDS 25.0%
! AAATAAATCGAAGATCAGTA+CGG + chr2_3:59223297-59223316 None:intergenic 25.0%
!! TACTGATCTTCGATTTATTT+TGG - chr2_3:59223297-59223316 Msa0302440:intron 25.0%
! ACAAACACAAATTAAACGAT+AGG + chr2_3:59223471-59223490 None:intergenic 25.0%
!! TTGTTAATTTGGATATTCCT+TGG - chr2_3:59223522-59223541 Msa0302440:intron 25.0%
!! TGTTAATTTGGATATTCCTT+GGG - chr2_3:59223523-59223542 Msa0302440:intron 25.0%
!! TGTTGTCTTGATAATATTGA+TGG - chr2_3:59224271-59224290 Msa0302440:intron 25.0%
!! GGATTTTTGTTAAGTTTCTA+TGG - chr2_3:59224669-59224688 Msa0302440:intron 25.0%
!!! TTTCTTTTTTCTCATCTCAA+GGG + chr2_3:59224834-59224853 None:intergenic 25.0%
!!! ATTTCTTTTTTCTCATCTCA+AGG + chr2_3:59224835-59224854 None:intergenic 25.0%
! AAAAATAACCCTTGATCAAT+GGG + chr2_3:59225004-59225023 None:intergenic 25.0%
!! GAATTAACGATTTTTGTCAT+AGG + chr2_3:59225164-59225183 None:intergenic 25.0%
GCTTAAAGCATATTCTTCTT+TGG - chr2_3:59221803-59221822 Msa0302440:three_prime_UTR 30.0%
TTGCATTATAAGAGCCTATT+AGG - chr2_3:59221832-59221851 Msa0302440:three_prime_UTR 30.0%
! TCTAGAACAACAAGAAGAAA+AGG - chr2_3:59221892-59221911 Msa0302440:three_prime_UTR 30.0%
CTAGAACAACAAGAAGAAAA+GGG - chr2_3:59221893-59221912 Msa0302440:three_prime_UTR 30.0%
TTTCACTGTGAAATTCATGT+TGG - chr2_3:59222157-59222176 Msa0302440:three_prime_UTR 30.0%
AACTAACTAGATCGGAAAAA+GGG + chr2_3:59222201-59222220 None:intergenic 30.0%
AAACTAACTAGATCGGAAAA+AGG + chr2_3:59222202-59222221 None:intergenic 30.0%
CATAGATCCTTATTCCATAT+AGG - chr2_3:59222332-59222351 Msa0302440:three_prime_UTR 30.0%
AGCATATCCTATATGGAATA+AGG + chr2_3:59222342-59222361 None:intergenic 30.0%
TTGTTTGAGCATATCCTATA+TGG + chr2_3:59222349-59222368 None:intergenic 30.0%
AAAAGAAAAACAGTTCCACA+AGG + chr2_3:59222423-59222442 None:intergenic 30.0%
!! GTGGAACTGTTTTTCTTTTT+AGG - chr2_3:59222424-59222443 Msa0302440:three_prime_UTR 30.0%
TTTACTGCTACGTAAATATG+CGG + chr2_3:59222794-59222813 None:intergenic 30.0%
AGGATATTGCAATATCAGTA+AGG + chr2_3:59223009-59223028 None:intergenic 30.0%
TCAGTGGTTTATTAATAGTG+TGG - chr2_3:59223424-59223443 Msa0302440:intron 30.0%
! TGCTGAAATGCTTGTTAATT+TGG - chr2_3:59223511-59223530 Msa0302440:CDS 30.0%
GCTTCTAAATGAAACAAATG+AGG - chr2_3:59223574-59223593 Msa0302440:intron 30.0%
!!! ACAAACTTTTTTGTTGTCTC+TGG - chr2_3:59223598-59223617 Msa0302440:intron 30.0%
!!! CAAACTTTTTTGTTGTCTCT+GGG - chr2_3:59223599-59223618 Msa0302440:intron 30.0%
GGATGATAAAGCAGTAAAAT+GGG + chr2_3:59223704-59223723 None:intergenic 30.0%
TTTGTCTGCAAGTAAAATTG+AGG + chr2_3:59224022-59224041 None:intergenic 30.0%
!! AAACTGGGATAATGTTGTTT+TGG - chr2_3:59224047-59224066 Msa0302440:intron 30.0%
AAGAATGAGATGTATCAACA+GGG + chr2_3:59224201-59224220 None:intergenic 30.0%
AAAGAATGAGATGTATCAAC+AGG + chr2_3:59224202-59224221 None:intergenic 30.0%
!!! ATATTGATGGTTTTTTGCCT+AGG - chr2_3:59224284-59224303 Msa0302440:intron 30.0%
ACCAAAAATCCATCAATATC+AGG + chr2_3:59224429-59224448 None:intergenic 30.0%
!!! GATATTGATGGATTTTTGGT+TGG - chr2_3:59224429-59224448 Msa0302440:intron 30.0%
TAAACACAAGTTCAAAGCAT+TGG + chr2_3:59224600-59224619 None:intergenic 30.0%
ACGTACAGTTAAGTAAAACA+CGG + chr2_3:59224742-59224761 None:intergenic 30.0%
!!! GCAGTTGTTTTTACTAAAAC+CGG - chr2_3:59224855-59224874 Msa0302440:intron 30.0%
CAAAAATAACCCTTGATCAA+TGG + chr2_3:59225005-59225024 None:intergenic 30.0%
!!! TCAAGGGTTATTTTTGTTCA+TGG - chr2_3:59225009-59225028 Msa0302440:intron 30.0%
AAACAAATATAATAGGGGAG+TGG - chr2_3:59225131-59225150 Msa0302440:five_prime_UTR 30.0%
AACAAATATAATAGGGGAGT+GGG - chr2_3:59225132-59225151 Msa0302440:five_prime_UTR 30.0%
TCTCTTAACAAAAATCGAAG+TGG - chr2_3:59225197-59225216 Msa0302440:five_prime_UTR 30.0%
! TCGTAATGTATAAGTTGAAG+TGG + chr2_3:59225229-59225248 None:intergenic 30.0%
ACTTCAACTTATACATTACG+AGG - chr2_3:59225228-59225247 Msa0302440:five_prime_UTR 30.0%
TTATAAGAGCCTATTAGGAC+AGG - chr2_3:59221837-59221856 Msa0302440:three_prime_UTR 35.0%
GGCAATTATGAATGCTAACA+TGG + chr2_3:59222402-59222421 None:intergenic 35.0%
TAGCATTCATAATTGCCTTG+TGG - chr2_3:59222405-59222424 Msa0302440:three_prime_UTR 35.0%
TTCTGGTGATTCATATTGGT+TGG - chr2_3:59222489-59222508 Msa0302440:three_prime_UTR 35.0%
! TCTGGTGATTCATATTGGTT+GGG - chr2_3:59222490-59222509 Msa0302440:three_prime_UTR 35.0%
TTACTGCTACGTAAATATGC+GGG + chr2_3:59222793-59222812 None:intergenic 35.0%
ATTCATTATACAGGCATGCA+TGG - chr2_3:59222843-59222862 Msa0302440:intron 35.0%
TTGTCAGCACATGCAAATAT+TGG + chr2_3:59223121-59223140 None:intergenic 35.0%
!! TGTGCTGACAAATGTTTTTC+TGG - chr2_3:59223130-59223149 Msa0302440:intron 35.0%
!!! ATGTTTTTCTGGTTCTACAG+AGG - chr2_3:59223141-59223160 Msa0302440:intron 35.0%
! CGAAGTAGACTTTTCACAAA+GGG + chr2_3:59223196-59223215 None:intergenic 35.0%
GTTCTGACACATGATTCAAT+CGG + chr2_3:59223378-59223397 None:intergenic 35.0%
AATCATGTGTCAGAACTGTT+TGG - chr2_3:59223382-59223401 Msa0302440:intron 35.0%
! TCTTTCTCGTTTTGTATCAG+TGG - chr2_3:59223408-59223427 Msa0302440:intron 35.0%
GATATTCCTTGGGTTATTCT+TGG - chr2_3:59223533-59223552 Msa0302440:intron 35.0%
CGGATGATAAAGCAGTAAAA+TGG + chr2_3:59223705-59223724 None:intergenic 35.0%
! TTGCAGACAAAGTATCAAAC+TGG - chr2_3:59224031-59224050 Msa0302440:intron 35.0%
! TGCAGACAAAGTATCAAACT+GGG - chr2_3:59224032-59224051 Msa0302440:intron 35.0%
TCCATAGATAATCCTTACAG+AGG + chr2_3:59224364-59224383 None:intergenic 35.0%
TCTGTAAGGATTATCTATGG+AGG - chr2_3:59224363-59224382 Msa0302440:intron 35.0%
ATCTATGGAGGTTCTGTAAA+CGG - chr2_3:59224375-59224394 Msa0302440:intron 35.0%
!!! GCCTGATATTGATGGATTTT+TGG - chr2_3:59224425-59224444 Msa0302440:intron 35.0%
!!! ATTGATGGATTTTTGGTTGG+CGG - chr2_3:59224432-59224451 Msa0302440:intron 35.0%
!! TTGATGATGTCAATGAACTC+TGG + chr2_3:59224471-59224490 None:intergenic 35.0%
! ATGTTTTCCCTTTACCTCAT+CGG - chr2_3:59224633-59224652 Msa0302440:intron 35.0%
CGTACAGTTAAGTAAAACAC+GGG + chr2_3:59224741-59224760 None:intergenic 35.0%
TATCATCAGACCTAAGACAA+TGG + chr2_3:59224771-59224790 None:intergenic 35.0%
! CTGATGATACTTGTGTTGTT+TGG - chr2_3:59224782-59224801 Msa0302440:intron 35.0%
TTGGATTGTGTTATGTCTTG+AGG - chr2_3:59224801-59224820 Msa0302440:intron 35.0%
TGTGTTATGTCTTGAGGATT+TGG - chr2_3:59224807-59224826 Msa0302440:intron 35.0%
! TACTACATGCTAGCTCTTTA+TGG + chr2_3:59225051-59225070 None:intergenic 35.0%
CTTATACATTACGAGGTTTC+AGG - chr2_3:59225235-59225254 Msa0302440:five_prime_UTR 35.0%
!! GAGGTTTCAGGTTTAAACTT+GGG - chr2_3:59225247-59225266 Msa0302440:five_prime_UTR 35.0%
ATGGGCAGAAAATTCTTCGT+CGG - chr2_3:59221955-59221974 Msa0302440:three_prime_UTR 40.0%
AGAGTGAAGAGAGTGAAAAG+AGG + chr2_3:59222009-59222028 None:intergenic 40.0%
CATATGTGTTAGTGTCGTCT+TGG - chr2_3:59222290-59222309 Msa0302440:three_prime_UTR 40.0%
! TCGTCTTGGATCAAAAGTGT+TGG - chr2_3:59222304-59222323 Msa0302440:three_prime_UTR 40.0%
!! GGGTTGATTTTGTTGCAGAA+TGG - chr2_3:59222510-59222529 Msa0302440:exon 40.0%
! GATTTTCTTCACCTCTTCAG+TGG + chr2_3:59222538-59222557 None:intergenic 40.0%
GAAGAGGTGAAGAAAATCGT+TGG - chr2_3:59222540-59222559 Msa0302440:CDS 40.0%
AATCGTTGGTACTTTGAACG+AGG - chr2_3:59222554-59222573 Msa0302440:CDS 40.0%
! ATGTTGTTGGTGAGTCTTAG+TGG - chr2_3:59222595-59222614 Msa0302440:CDS 40.0%
AAAGGGAAGGAACACATAAG+GGG + chr2_3:59223179-59223198 None:intergenic 40.0%
CAAAGGGAAGGAACACATAA+GGG + chr2_3:59223180-59223199 None:intergenic 40.0%
ACAAAGGGAAGGAACACATA+AGG + chr2_3:59223181-59223200 None:intergenic 40.0%
! GTAGACTTTTCACAAAGGGA+AGG + chr2_3:59223192-59223211 None:intergenic 40.0%
! GCGAAGTAGACTTTTCACAA+AGG + chr2_3:59223197-59223216 None:intergenic 40.0%
GATAACATACCTAATCTCCC+CGG + chr2_3:59223275-59223294 None:intergenic 40.0%
! TTTTTATCATCACTGCCGCA+AGG - chr2_3:59223334-59223353 Msa0302440:intron 40.0%
! TTTTATCATCACTGCCGCAA+GGG - chr2_3:59223335-59223354 Msa0302440:intron 40.0%
TCATACACAGCTAGCACAAA+GGG + chr2_3:59223641-59223660 None:intergenic 40.0%
TTATCATCCGCTACAGTTTG+TGG - chr2_3:59223715-59223734 Msa0302440:intron 40.0%
TATCATCCGCTACAGTTTGT+GGG - chr2_3:59223716-59223735 Msa0302440:intron 40.0%
TTGCATATGCACTCTCACTA+GGG - chr2_3:59223747-59223766 Msa0302440:intron 40.0%
AGGAAAGTCATTGCTTGCAT+TGG - chr2_3:59223770-59223789 Msa0302440:CDS 40.0%
GGAAAGTCATTGCTTGCATT+GGG - chr2_3:59223771-59223790 Msa0302440:CDS 40.0%
GAAAGTCATTGCTTGCATTG+GGG - chr2_3:59223772-59223791 Msa0302440:CDS 40.0%
TGTTGTTGCTGAGCAAACAA+AGG - chr2_3:59223832-59223851 Msa0302440:CDS 40.0%
!!! TTCATCACTTGCTGTTTTGC+TGG - chr2_3:59223977-59223996 Msa0302440:intron 40.0%
! TTTTGGCATATGAGCCAGTT+TGG - chr2_3:59224064-59224083 Msa0302440:intron 40.0%
TTTGGCATATGAGCCAGTTT+GGG - chr2_3:59224065-59224084 Msa0302440:intron 40.0%
!! TCCATGCTGCTTTAAGGAAA+TGG - chr2_3:59224307-59224326 Msa0302440:intron 40.0%
!! CCATGCTGCTTTAAGGAAAT+GGG - chr2_3:59224308-59224327 Msa0302440:intron 40.0%
GCCTCTGTAAGGATTATCTA+TGG - chr2_3:59224360-59224379 Msa0302440:intron 40.0%
ACTTCAGTTCTTCTTCACTG+TGG + chr2_3:59224499-59224518 None:intergenic 40.0%
TTAAGTAAAACACGGGCGTT+CGG + chr2_3:59224734-59224753 None:intergenic 40.0%
ACGTTTATCGCCATTGTCTT+AGG - chr2_3:59224758-59224777 Msa0302440:intron 40.0%
! TGATGACGACAATTCACTTC+CGG + chr2_3:59224877-59224896 None:intergenic 40.0%
GAATTGTCGTCATCACAGAA+AGG - chr2_3:59224882-59224901 Msa0302440:intron 40.0%
TCTCTGCTGAACTATGTTCT+AGG - chr2_3:59224907-59224926 Msa0302440:intron 40.0%
CTCTGCTGAACTATGTTCTA+GGG - chr2_3:59224908-59224927 Msa0302440:intron 40.0%
!! CGAGGTTTCAGGTTTAAACT+TGG - chr2_3:59225246-59225265 Msa0302440:five_prime_UTR 40.0%
AATTCTTCGTCGGTGGTAAC+TGG - chr2_3:59221965-59221984 Msa0302440:three_prime_UTR 45.0%
TCACCAACAACATCTTCTCC+GGG + chr2_3:59222588-59222607 None:intergenic 45.0%
CTCACCAACAACATCTTCTC+CGG + chr2_3:59222589-59222608 None:intergenic 45.0%
TAAATATGCGGGTTCCCTCT+TGG + chr2_3:59222782-59222801 None:intergenic 45.0%
AAGGGAAGGAACACATAAGG+GGG + chr2_3:59223178-59223197 None:intergenic 45.0%
!! AAGCGTTTTGAGCAGCAACA+TGG + chr2_3:59223228-59223247 None:intergenic 45.0%
ATGTTGCTGCTCAAAACGCT+TGG - chr2_3:59223227-59223246 Msa0302440:CDS 45.0%
TGTTGCTGCTCAAAACGCTT+GGG - chr2_3:59223228-59223247 Msa0302440:CDS 45.0%
CATACACAGCTAGCACAAAG+GGG + chr2_3:59223640-59223659 None:intergenic 45.0%
GTCATACACAGCTAGCACAA+AGG + chr2_3:59223642-59223661 None:intergenic 45.0%
TTGTCTCCCACAAACTGTAG+CGG + chr2_3:59223725-59223744 None:intergenic 45.0%
GTTGCATATGCACTCTCACT+AGG - chr2_3:59223746-59223765 Msa0302440:intron 45.0%
ACTCTTGAACAACGTGAAGC+TGG - chr2_3:59223797-59223816 Msa0302440:CDS 45.0%
GAGCAAACAAAGGCAATTGC+AGG - chr2_3:59223842-59223861 Msa0302440:CDS 45.0%
CCCATTTCCTTAAAGCAGCA+TGG + chr2_3:59224311-59224330 None:intergenic 45.0%
! AAATGGGTTCACGACAACGT+AGG - chr2_3:59224324-59224343 Msa0302440:intron 45.0%
TATGGAGGTTCTGTAAACGG+AGG - chr2_3:59224378-59224397 Msa0302440:intron 45.0%
CGGAGGAAACTCCAAAGAAT+TGG - chr2_3:59224395-59224414 Msa0302440:intron 45.0%
AGGCTGAGATGCCAATTCTT+TGG + chr2_3:59224409-59224428 None:intergenic 45.0%
GCATCTCAGCCTGATATTGA+TGG - chr2_3:59224417-59224436 Msa0302440:intron 45.0%
! GTCAATGAACTCTGGCTTCA+AGG + chr2_3:59224463-59224482 None:intergenic 45.0%
CAGAAATTTCCGAAGCAGCT+TGG + chr2_3:59224550-59224569 None:intergenic 45.0%
! TTTTCCCTTTACCTCATCGG+TGG - chr2_3:59224636-59224655 Msa0302440:intron 45.0%
TTTCCCTTTACCTCATCGGT+GGG - chr2_3:59224637-59224656 Msa0302440:intron 45.0%
GTCGTGACTGCCATAACATT+TGG - chr2_3:59224696-59224715 Msa0302440:intron 45.0%
GAATCAATTTGGCGACAGAC+CGG - chr2_3:59224958-59224977 Msa0302440:intron 45.0%
AGGCTGAACCCATTGATCAA+GGG - chr2_3:59224993-59225012 Msa0302440:intron 45.0%
!! ATATTTAGTTAAAAATTTAA+GGG + chr2_3:59222951-59222970 None:intergenic 5.0%
!! TTATTATAAACAAATATAAT+AGG - chr2_3:59225124-59225143 Msa0302440:five_prime_UTR 5.0%
!! TATTATAAACAAATATAATA+GGG - chr2_3:59225125-59225144 Msa0302440:five_prime_UTR 5.0%
AGAGCCTATTAGGACAGGAC+AGG - chr2_3:59221842-59221861 Msa0302440:three_prime_UTR 50.0%
CTGTCCTGTCCTGTCCTAAT+AGG + chr2_3:59221849-59221868 None:intergenic 50.0%
GGCAGAAAATTCTTCGTCGG+TGG - chr2_3:59221958-59221977 Msa0302440:three_prime_UTR 50.0%
TGACACGTACACGTTGACAC+CGG - chr2_3:59222232-59222251 Msa0302440:intron 50.0%
GCAGAATGGAACCACTGAAG+AGG - chr2_3:59222524-59222543 Msa0302440:exon 50.0%
TTGAACGAGGCTCAAGTTCC+CGG - chr2_3:59222567-59222586 Msa0302440:CDS 50.0%
GTTCCCGGAGAAGATGTTGT+TGG - chr2_3:59222582-59222601 Msa0302440:CDS 50.0%
GGCAACTGCAGTGAATTCAG+TGG + chr2_3:59222988-59223007 None:intergenic 50.0%
AGGGAAGGAACACATAAGGG+GGG + chr2_3:59223177-59223196 None:intergenic 50.0%
CAAAACGCTTGGGTTCGCAA+AGG - chr2_3:59223238-59223257 Msa0302440:CDS 50.0%
CGAAAAGACCGAAACCCTTG+CGG + chr2_3:59223352-59223371 None:intergenic 50.0%
GAGTGGCCAAGAATAACCCA+AGG + chr2_3:59223542-59223561 None:intergenic 50.0%
CATATGCACTCTCACTAGGG+AGG - chr2_3:59223750-59223769 Msa0302440:intron 50.0%
TATGAGCCAGTTTGGGCCAT+TGG - chr2_3:59224072-59224091 Msa0302440:intron 50.0%
ATGAGCCAGTTTGGGCCATT+GGG - chr2_3:59224073-59224092 Msa0302440:intron 50.0%
AGCAACCTTTCCAGTCCCAA+TGG + chr2_3:59224091-59224110 None:intergenic 50.0%
AAAGGTTGCTTCTCCTGCTC+AGG - chr2_3:59224101-59224120 Msa0302440:intron 50.0%
CCTTAAAGCAGCATGGACCT+AGG + chr2_3:59224304-59224323 None:intergenic 50.0%
!! CCTAGGTCCATGCTGCTTTA+AGG - chr2_3:59224301-59224320 Msa0302440:intron 50.0%
CTGAAGTTGCTGCCTCTGTA+AGG - chr2_3:59224349-59224368 Msa0302440:intron 50.0%
!! TGCTGCTTTCCAAGCTGCTT+CGG - chr2_3:59224538-59224557 Msa0302440:intron 50.0%
CAGGCTGAACCCATTGATCA+AGG - chr2_3:59224992-59225011 Msa0302440:intron 50.0%
AACGCTTGGGTTCGCAAAGG+TGG - chr2_3:59223241-59223260 Msa0302440:CDS 55.0%
CGCAAAGGTGGTGCTTACAC+CGG - chr2_3:59223253-59223272 Msa0302440:CDS 55.0%
GCAAAGGTGGTGCTTACACC+GGG - chr2_3:59223254-59223273 Msa0302440:CDS 55.0%
CAAAGGTGGTGCTTACACCG+GGG - chr2_3:59223255-59223274 Msa0302440:CDS 55.0%
GTGCTTACACCGGGGAGATT+AGG - chr2_3:59223263-59223282 Msa0302440:CDS 55.0%
CATCACTGCCGCAAGGGTTT+CGG - chr2_3:59223341-59223360 Msa0302440:intron 55.0%
TTCTTGGCCACTCTGAGCGA+AGG - chr2_3:59223549-59223568 Msa0302440:intron 55.0%
TTGGGCCATTGGGACTGGAA+AGG - chr2_3:59224083-59224102 Msa0302440:intron 55.0%
TTCTCCTGCTCAGGCTCAAG+AGG - chr2_3:59224110-59224129 Msa0302440:intron 55.0%
ACTACCTCTTGAGCCTGAGC+AGG + chr2_3:59224117-59224136 None:intergenic 55.0%
TCGCCCACCGATGAGGTAAA+GGG + chr2_3:59224643-59224662 None:intergenic 55.0%
ATCGCCCACCGATGAGGTAA+AGG + chr2_3:59224644-59224663 None:intergenic 55.0%
CAATTCATCGCCCACCGATG+AGG + chr2_3:59224650-59224669 None:intergenic 55.0%
CTCATCGGTGGGCGATGAAT+TGG - chr2_3:59224648-59224667 Msa0302440:intron 55.0%
CAATTTGGCGACAGACCGGT+TGG - chr2_3:59224962-59224981 Msa0302440:intron 55.0%
TCAGCCTGTCCGTAACCAAC+CGG + chr2_3:59224980-59224999 None:intergenic 55.0%
GAAGCTGCCTTCGCTCAGAG+TGG + chr2_3:59223559-59223578 None:intergenic 60.0%
CCAGTCCCAATGGCCCAAAC+TGG + chr2_3:59224081-59224100 None:intergenic 60.0%
CCAGTTTGGGCCATTGGGAC+TGG - chr2_3:59224078-59224097 Msa0302440:intron 60.0%
! GGCGACAGACCGGTTGGTTA+CGG - chr2_3:59224968-59224987 Msa0302440:intron 60.0%
! CAGACCGGTTGGTTACGGAC+AGG - chr2_3:59224973-59224992 Msa0302440:intron 60.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 59221763 59225280 59221763 ID=Msa0302440;Name=Msa0302440
chr2_3 mRNA 59221763 59225280 59221763 ID=Msa0302440-mRNA-1;Parent=Msa0302440;Name=Msa0302440-mRNA-1;_AED=0.08;_eAED=0.08;_QI=192|1|1|1|0.85|0.75|8|740|253
chr2_3 exon 59221763 59222238 59221763 ID=Msa0302440-mRNA-1:exon:19178;Parent=Msa0302440-mRNA-1
chr2_3 exon 59222264 59222737 59222264 ID=Msa0302440-mRNA-1:exon:19177;Parent=Msa0302440-mRNA-1
chr2_3 exon 59222912 59223006 59222912 ID=Msa0302440-mRNA-1:exon:19176;Parent=Msa0302440-mRNA-1
chr2_3 exon 59223180 59223312 59223180 ID=Msa0302440-mRNA-1:exon:19175;Parent=Msa0302440-mRNA-1
chr2_3 exon 59223448 59223532 59223448 ID=Msa0302440-mRNA-1:exon:19174;Parent=Msa0302440-mRNA-1
chr2_3 exon 59223759 59223882 59223759 ID=Msa0302440-mRNA-1:exon:19173;Parent=Msa0302440-mRNA-1
chr2_3 exon 59224440 59224515 59224440 ID=Msa0302440-mRNA-1:exon:19172;Parent=Msa0302440-mRNA-1
chr2_3 exon 59225050 59225280 59225050 ID=Msa0302440-mRNA-1:exon:19171;Parent=Msa0302440-mRNA-1
chr2_3 five_prime_UTR 59225089 59225280 59225089 ID=Msa0302440-mRNA-1:five_prime_utr;Parent=Msa0302440-mRNA-1
chr2_3 CDS 59225050 59225088 59225050 ID=Msa0302440-mRNA-1:cds;Parent=Msa0302440-mRNA-1
chr2_3 CDS 59224440 59224515 59224440 ID=Msa0302440-mRNA-1:cds;Parent=Msa0302440-mRNA-1
chr2_3 CDS 59223759 59223882 59223759 ID=Msa0302440-mRNA-1:cds;Parent=Msa0302440-mRNA-1
chr2_3 CDS 59223448 59223532 59223448 ID=Msa0302440-mRNA-1:cds;Parent=Msa0302440-mRNA-1
chr2_3 CDS 59223180 59223312 59223180 ID=Msa0302440-mRNA-1:cds;Parent=Msa0302440-mRNA-1
chr2_3 CDS 59222912 59223006 59222912 ID=Msa0302440-mRNA-1:cds;Parent=Msa0302440-mRNA-1
chr2_3 CDS 59222528 59222737 59222528 ID=Msa0302440-mRNA-1:cds;Parent=Msa0302440-mRNA-1
chr2_3 three_prime_UTR 59222264 59222527 59222264 ID=Msa0302440-mRNA-1:three_prime_utr;Parent=Msa0302440-mRNA-1
chr2_3 three_prime_UTR 59221763 59222238 59221763 ID=Msa0302440-mRNA-1:three_prime_utr;Parent=Msa0302440-mRNA-1
Gene Sequence

>Msa0302440

ATGGGCAGAAAATTCTTCGTCGGTGGTAACTGGAAATGCAATGGAACCACTGAAGAGGTGAAGAAAATCGTTGGTACTTTGAACGAGGCTCAAGTTCCCGGAGAAGATGTTGTTGAGGTTGTTGTGAGCCCCCCTTATGTGTTCCTTCCCTTTGTGAAAAGTCTACTTCGCTCTGACTTCCATGTTGCTGCTCAAAACGCTTGGGTTCGCAAAGGTGGTGCTTACACCGGGGAGATTAGTGCTGAAATGCTTGTTAATTTGGATATTCCTTGGGTTATTCTTGGCCACTCTGAGCGAAGGCAGCTTCTAAATGAAACAAATGAGTTTGTGGGAGACAAAGTTGCATATGCACTCTCACTAGGGAGGAAAGTCATTGCTTGCATTGGGGAGACTCTTGAACAACGTGAAGCTGGTACTACAATAGCTGTTGTTGCTGAGCAAACAAAGGCAATTGCAGACAAAGTATCAAACTGGGATAATGTTGTTTTGGCATATGAGCCAGTTTGGGCCATTGGGACTGGAAAGGTTGCTTCTCCTGCTCAGGCTCAAGAGGTCCATGCTGCTTTAAGGAAATGGGTTCACGACAACGTAGGTGCTGAAGTTGCTGCCTCTGTAAGGATTATCTATGGAGGTTCTGTAAACGGAGGAAACTCCAAAGAATTGGCATCTCAGCCTGATATTGATGGATTTTTGGTTGGCGGTGCTTCCTTGAAGCCAGAGTTCATTGACATCATCAACTCTGCCACAGTGAAGAAGAACTGA
Protein sequence

>Msa0302440

MGRKFFVGGNWKCNGTTEEVKKIVGTLNEAQVPGEDVVEVVVSPPYVFLPFVKSLLRSDFHVAAQNAWVRKGGAYTGEISAEMLVNLDIPWVILGHSERRQLLNETNEFVGDKVAYALSLGRKVIACIGETLEQREAGTTIAVVAEQTKAIADKVSNWDNVVLAYEPVWAIGTGKVASPAQAQEVHAALRKWVHDNVGAEVAASVRIIYGGSVNGGNSKELASQPDIDGFLVGGASLKPEFIDIINSATVKKN*