AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0339590

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0339590 MtrunA17_Chr2g0328161 100.000 253 0 0 1 253 1 253 0.0 513
Msa0339590 MtrunA17_Chr7g0264951 63.200 250 89 2 4 252 62 309 3.99e-110 320
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0339590 AT3G55440.1 81.423 253 47 0 1 253 1 253 4.40e-154 429
Msa0339590 AT2G21170.3 63.600 250 88 2 4 252 65 312 1.79e-109 318
Msa0339590 AT2G21170.1 63.600 250 88 2 4 252 65 312 1.79e-109 318
Msa0339590 AT2G21170.2 62.083 240 88 2 14 252 66 303 6.90e-100 293

Find 118 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAGGTCCATGCTGCTTTA+AGG 0.106869 2_4:+60793049 Msa0339590:intron
TGCTGAAATGCTTGTTAATT+TGG 0.126755 2_4:+60792259 Msa0339590:CDS
TACTACATGCTAGCTCTTTA+TGG 0.167583 2_4:-60793795 None:intergenic
CTTATACATTACGAGGTTTC+AGG 0.215165 2_4:+60793982 Msa0339590:three_prime_UTR
TTATTATAAACAAATATAAT+AGG 0.225661 2_4:+60793871 Msa0339590:three_prime_UTR
GATATTCCTTGGGTTATTCT+TGG 0.231384 2_4:+60792281 Msa0339590:CDS
TTGCATTATAAGAGCCTATT+AGG 0.248380 2_4:+60790597 Msa0339590:five_prime_UTR
GCTTAAAGCATATTCTTCTT+TGG 0.258020 2_4:+60790568 Msa0339590:five_prime_UTR
ATAAGTGTATTGAATCAATT+TGG 0.259889 2_4:+60793694 Msa0339590:three_prime_UTR
TGTGTTATGTCTTGAGGATT+TGG 0.279401 2_4:+60793555 Msa0339590:three_prime_UTR
AAACTGGGATAATGTTGTTT+TGG 0.298614 2_4:+60792795 Msa0339590:CDS
TCTCTGCTGAACTATGTTCT+AGG 0.305712 2_4:+60793654 Msa0339590:three_prime_UTR
ACGTTTATCGCCATTGTCTT+AGG 0.313974 2_4:+60793506 Msa0339590:three_prime_UTR
TCCATGCTGCTTTAAGGAAA+TGG 0.316327 2_4:+60793055 Msa0339590:CDS
CTCATCGGTGGGCGATGAAT+TGG 0.317824 2_4:+60793396 Msa0339590:three_prime_UTR
AGGCTGAGATGCCAATTCTT+TGG 0.323629 2_4:-60793154 None:intergenic
AATTCTTCGTCGGTGGTAAC+TGG 0.328227 2_4:+60790730 Msa0339590:CDS
CTGTCCTGTCCTGTCCTAAT+AGG 0.332728 2_4:-60790611 None:intergenic
TATTATAAACAAATATAATA+GGG 0.346860 2_4:+60793872 Msa0339590:three_prime_UTR
TGTTAATTTGGATATTCCTT+GGG 0.361669 2_4:+60792271 Msa0339590:CDS
GCATCTCAGCCTGATATTGA+TGG 0.369973 2_4:+60793165 Msa0339590:CDS
TTGCAGACAAAGTATCAAAC+TGG 0.370984 2_4:+60792779 Msa0339590:intron
TTGTTAATTTGGATATTCCT+TGG 0.377981 2_4:+60792270 Msa0339590:CDS
GGAAAGTCATTGCTTGCATT+GGG 0.383771 2_4:+60792519 Msa0339590:CDS
TTTGGCATATGAGCCAGTTT+GGG 0.383886 2_4:+60792813 Msa0339590:CDS
GCCTCTGTAAGGATTATCTA+TGG 0.388025 2_4:+60793108 Msa0339590:CDS
CCAGTTTGGGCCATTGGGAC+TGG 0.396885 2_4:+60792826 Msa0339590:CDS
CGGAGGAAACTCCAAAGAAT+TGG 0.404230 2_4:+60793143 Msa0339590:CDS
CAAAGGGAAGGAACACATAA+GGG 0.405048 2_4:-60791925 None:intergenic
TATGAGCCAGTTTGGGCCAT+TGG 0.406432 2_4:+60792820 Msa0339590:CDS
GTCAATGAACTCTGGCTTCA+AGG 0.409151 2_4:-60793208 None:intergenic
CGAGGTTTCAGGTTTAAACT+TGG 0.409231 2_4:+60793993 Msa0339590:three_prime_UTR
TCTAGAACAACAAGAAGAAA+AGG 0.412429 2_4:+60790657 Msa0339590:five_prime_UTR
GTCGTGACTGCCATAACATT+TGG 0.413861 2_4:+60793444 Msa0339590:three_prime_UTR
GTGCTTACACCGGGGAGATT+AGG 0.414986 2_4:+60792011 Msa0339590:CDS
ATCTATGGAGGTTCTGTAAA+CGG 0.416225 2_4:+60793123 Msa0339590:CDS
ATGAGCCAGTTTGGGCCATT+GGG 0.421695 2_4:+60792821 Msa0339590:CDS
TTGAACGAGGCTCAAGTTCC+CGG 0.427132 2_4:+60791316 Msa0339590:CDS
TTATTACTAACCAAATGTTA+TGG 0.428730 2_4:-60793454 None:intergenic
TTAAGTAAAACACGGGCGTT+CGG 0.431142 2_4:-60793479 None:intergenic
CCAGTCCCAATGGCCCAAAC+TGG 0.435261 2_4:-60792826 None:intergenic
CCATGCTGCTTTAAGGAAAT+GGG 0.440149 2_4:+60793056 Msa0339590:CDS
AAAGGTTGCTTCTCCTGCTC+AGG 0.446019 2_4:+60792849 Msa0339590:CDS
TGCTGCTTTCCAAGCTGCTT+CGG 0.446387 2_4:+60793286 Msa0339590:three_prime_UTR
GGCGACAGACCGGTTGGTTA+CGG 0.448337 2_4:+60793715 Msa0339590:three_prime_UTR
GAGCAAACAAAGGCAATTGC+AGG 0.449292 2_4:+60792590 Msa0339590:CDS
AGGAAAGTCATTGCTTGCAT+TGG 0.454029 2_4:+60792518 Msa0339590:CDS
TGTTGCTGCTCAAAACGCTT+GGG 0.460320 2_4:+60791976 Msa0339590:CDS
CAGAAATTTCCGAAGCAGCT+TGG 0.467398 2_4:-60793295 None:intergenic
CTGAAGTTGCTGCCTCTGTA+AGG 0.468253 2_4:+60793097 Msa0339590:CDS
TATCATCCGCTACAGTTTGT+GGG 0.468679 2_4:+60792464 Msa0339590:intron
ATCGCCCACCGATGAGGTAA+AGG 0.469952 2_4:-60793389 None:intergenic
CTCACCAACAACATCTTCTC+CGG 0.472241 2_4:-60791335 None:intergenic
GCAAAGGTGGTGCTTACACC+GGG 0.472783 2_4:+60792002 Msa0339590:CDS
AACAAATATAATAGGGGAGT+GGG 0.482103 2_4:+60793879 Msa0339590:three_prime_UTR
ATGTTGCTGCTCAAAACGCT+TGG 0.482349 2_4:+60791975 Msa0339590:CDS
CAGGCTGAACCCATTGATCA+AGG 0.495327 2_4:+60793739 Msa0339590:three_prime_UTR
GTTGCATATGCACTCTCACT+AGG 0.495343 2_4:+60792494 Msa0339590:CDS
CGCAAAGGTGGTGCTTACAC+CGG 0.498016 2_4:+60792001 Msa0339590:CDS
CTAGAACAACAAGAAGAAAA+GGG 0.510864 2_4:+60790658 Msa0339590:five_prime_UTR
TCAGCCTGTCCGTAACCAAC+CGG 0.512292 2_4:-60793724 None:intergenic
TTGGATTGTGTTATGTCTTG+AGG 0.512893 2_4:+60793549 Msa0339590:intron
TCGCCCACCGATGAGGTAAA+GGG 0.517509 2_4:-60793388 None:intergenic
AAACAAATATAATAGGGGAG+TGG 0.521187 2_4:+60793878 Msa0339590:three_prime_UTR
AACGCTTGGGTTCGCAAAGG+TGG 0.525911 2_4:+60791989 Msa0339590:CDS
ACTACCTCTTGAGCCTGAGC+AGG 0.528540 2_4:-60792862 None:intergenic
ACTCTTGAACAACGTGAAGC+TGG 0.531573 2_4:+60792545 Msa0339590:CDS
ACAAAGGGAAGGAACACATA+AGG 0.532183 2_4:-60791926 None:intergenic
TTCTCCTGCTCAGGCTCAAG+AGG 0.539203 2_4:+60792858 Msa0339590:CDS
GTTCCCGGAGAAGATGTTGT+TGG 0.540063 2_4:+60791331 Msa0339590:CDS
CTCTGCTGAACTATGTTCTA+GGG 0.543887 2_4:+60793655 Msa0339590:three_prime_UTR
TAAACACAAGTTCAAAGCAT+TGG 0.547440 2_4:-60793345 None:intergenic
GAGGTTTCAGGTTTAAACTT+GGG 0.552233 2_4:+60793994 Msa0339590:three_prime_UTR
ATTATAAACAAATATAATAG+GGG 0.554042 2_4:+60793873 Msa0339590:three_prime_UTR
TTGGGCCATTGGGACTGGAA+AGG 0.554351 2_4:+60792831 Msa0339590:CDS
CCCATTTCCTTAAAGCAGCA+TGG 0.555610 2_4:-60793056 None:intergenic
GAATCAATTTGGCGACAGAC+CGG 0.559054 2_4:+60793705 Msa0339590:three_prime_UTR
CAATTTGGCGACAGACCGGT+TGG 0.561018 2_4:+60793709 Msa0339590:three_prime_UTR
TCACCAACAACATCTTCTCC+GGG 0.561852 2_4:-60791334 None:intergenic
TTCTTGGCCACTCTGAGCGA+AGG 0.565910 2_4:+60792297 Msa0339590:CDS
TTATAAGAGCCTATTAGGAC+AGG 0.566780 2_4:+60790602 Msa0339590:five_prime_UTR
TTTCCCTTTACCTCATCGGT+GGG 0.569814 2_4:+60793385 Msa0339590:three_prime_UTR
TCGTAATGTATAAGTTGAAG+TGG 0.578365 2_4:-60793973 None:intergenic
TTGATGATGTCAATGAACTC+TGG 0.578436 2_4:-60793216 None:intergenic
GCTTCTAAATGAAACAAATG+AGG 0.602961 2_4:+60792322 Msa0339590:CDS
ACTTCAACTTATACATTACG+AGG 0.604440 2_4:+60793975 Msa0339590:three_prime_UTR
TATCATCAGACCTAAGACAA+TGG 0.607431 2_4:-60793516 None:intergenic
AGAGCCTATTAGGACAGGAC+AGG 0.608412 2_4:+60790607 Msa0339590:five_prime_UTR
GAAGAGGTGAAGAAAATCGT+TGG 0.610298 2_4:+60791289 Msa0339590:CDS
AGCAACCTTTCCAGTCCCAA+TGG 0.611585 2_4:-60792836 None:intergenic
CAATTCATCGCCCACCGATG+AGG 0.616929 2_4:-60793395 None:intergenic
ATGGGCAGAAAATTCTTCGT+CGG 0.618039 2_4:+60790720 Msa0339590:CDS
TTGCATATGCACTCTCACTA+GGG 0.619513 2_4:+60792495 Msa0339590:CDS
GAATTGTCGTCATCACAGAA+AGG 0.621483 2_4:+60793629 Msa0339590:three_prime_UTR
AAAGGGAAGGAACACATAAG+GGG 0.623658 2_4:-60791924 None:intergenic
GGCAGAAAATTCTTCGTCGG+TGG 0.629014 2_4:+60790723 Msa0339590:CDS
GAAAGTCATTGCTTGCATTG+GGG 0.633688 2_4:+60792520 Msa0339590:CDS
AGGCTGAACCCATTGATCAA+GGG 0.634403 2_4:+60793740 Msa0339590:three_prime_UTR
AAGGGAAGGAACACATAAGG+GGG 0.637952 2_4:-60791923 None:intergenic
TGCAGACAAAGTATCAAACT+GGG 0.639857 2_4:+60792780 Msa0339590:intron
CAGACCGGTTGGTTACGGAC+AGG 0.641537 2_4:+60793720 Msa0339590:three_prime_UTR
CAAAACGCTTGGGTTCGCAA+AGG 0.648775 2_4:+60791986 Msa0339590:CDS
TGTTGTTGCTGAGCAAACAA+AGG 0.653424 2_4:+60792580 Msa0339590:CDS
TCTGTAAGGATTATCTATGG+AGG 0.664957 2_4:+60793111 Msa0339590:CDS
ACGTACAGTTAAGTAAAACA+CGG 0.667629 2_4:-60793487 None:intergenic
TCCATAGATAATCCTTACAG+AGG 0.672902 2_4:-60793109 None:intergenic
GCAGAATGGAACCACTGAAG+AGG 0.675685 2_4:+60791273 Msa0339590:intron
CATATGCACTCTCACTAGGG+AGG 0.678402 2_4:+60792498 Msa0339590:CDS
GAAGCTGCCTTCGCTCAGAG+TGG 0.679228 2_4:-60792304 None:intergenic
GAGTGGCCAAGAATAACCCA+AGG 0.679606 2_4:-60792287 None:intergenic
CGTACAGTTAAGTAAAACAC+GGG 0.684223 2_4:-60793486 None:intergenic
GATAACATACCTAATCTCCC+CGG 0.689404 2_4:-60792020 None:intergenic
TATGGAGGTTCTGTAAACGG+AGG 0.700353 2_4:+60793126 Msa0339590:CDS
AATCGTTGGTACTTTGAACG+AGG 0.707863 2_4:+60791303 Msa0339590:CDS
AAATGGGTTCACGACAACGT+AGG 0.725939 2_4:+60793072 Msa0339590:CDS
ACTTCAGTTCTTCTTCACTG+TGG 0.744514 2_4:-60793244 None:intergenic
AGGGAAGGAACACATAAGGG+GGG 0.750761 2_4:-60791922 None:intergenic
CAAAGGTGGTGCTTACACCG+GGG 0.762885 2_4:+60792003 Msa0339590:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATATTTAGTTAAAAATTTA+AGG - chr2_4:60791700-60791719 None:intergenic 10.0%
!!! TCAATAAAAATAATTTTTGT+TGG - chr2_4:60791827-60791846 None:intergenic 10.0%
!!! AAATTTTAAAAACAGTATAT+AGG - chr2_4:60792892-60792911 None:intergenic 10.0%
!! ATTATAAACAAATATAATAG+GGG + chr2_4:60793873-60793892 Msa0339590:three_prime_UTR 10.0%
!! TGATGAAATAAATCAAATAA+TGG - chr2_4:60792712-60792731 None:intergenic 15.0%
!! GTTTCAGAATTAAGATTTAT+AGG - chr2_4:60790873-60790892 None:intergenic 20.0%
!! AACTATGTTAAAAATCAGAT+CGG + chr2_4:60790890-60790909 Msa0339590:intron 20.0%
!! GTATATAGACTATATATTTC+TGG + chr2_4:60791221-60791240 Msa0339590:intron 20.0%
!!! AGTAAATTTCAGTATTGATT+TGG + chr2_4:60791445-60791464 Msa0339590:intron 20.0%
!!! ATAAGAAATTTTTTTCCAAG+AGG + chr2_4:60791513-60791532 Msa0339590:intron 20.0%
!!! TAAGAAATTTTTTTCCAAGA+GGG + chr2_4:60791514-60791533 Msa0339590:intron 20.0%
!!! TCTCATTTTAATAATGCAAA+GGG - chr2_4:60791566-60791585 None:intergenic 20.0%
!! TTAACTAAATATCAAGTTTC+TGG + chr2_4:60791707-60791726 Msa0339590:intron 20.0%
!! TACAGAGATACAAAAAAAAA+AGG - chr2_4:60791777-60791796 None:intergenic 20.0%
!! TTATTACTAACCAAATGTTA+TGG - chr2_4:60793457-60793476 None:intergenic 20.0%
!! ATAAGTGTATTGAATCAATT+TGG + chr2_4:60793694-60793713 Msa0339590:three_prime_UTR 20.0%
! AATCATCGAAATCAAAAACA+TGG + chr2_4:60790701-60790720 Msa0339590:exon 25.0%
! ATCATCGAAATCAAAAACAT+GGG + chr2_4:60790702-60790721 Msa0339590:exon 25.0%
! CAACTAAAAACTAACTAGAT+CGG - chr2_4:60790975-60790994 None:intergenic 25.0%
!!! ATATTTCTGGTGATTCATAT+TGG + chr2_4:60791234-60791253 Msa0339590:intron 25.0%
!!! GTCTCATTTTAATAATGCAA+AGG - chr2_4:60791567-60791586 None:intergenic 25.0%
! GAGACTTAAATTCATTATAC+AGG + chr2_4:60791582-60791601 Msa0339590:intron 25.0%
!! ACGAAATTTGCTTATCTATT+TGG + chr2_4:60791673-60791692 Msa0339590:intron 25.0%
! AAATAAATCGAAGATCAGTA+CGG - chr2_4:60792045-60792064 None:intergenic 25.0%
!! TACTGATCTTCGATTTATTT+TGG + chr2_4:60792045-60792064 Msa0339590:intron 25.0%
! ACAAACACAAATTAAACGAT+AGG - chr2_4:60792219-60792238 None:intergenic 25.0%
!! TTGTTAATTTGGATATTCCT+TGG + chr2_4:60792270-60792289 Msa0339590:CDS 25.0%
!! TGTTAATTTGGATATTCCTT+GGG + chr2_4:60792271-60792290 Msa0339590:CDS 25.0%
!! TGTTGTCTTGATAATATTGA+TGG + chr2_4:60793019-60793038 Msa0339590:intron 25.0%
!! GGATTTTTGTTAAGTTTCTA+TGG + chr2_4:60793417-60793436 Msa0339590:three_prime_UTR 25.0%
!!! TTTCTTTTTTCTCATCTCAA+GGG - chr2_4:60793582-60793601 None:intergenic 25.0%
!!! ATTTCTTTTTTCTCATCTCA+AGG - chr2_4:60793583-60793602 None:intergenic 25.0%
!!! TGCAGTTGTTTTTACTAAAA+CGG + chr2_4:60793602-60793621 Msa0339590:three_prime_UTR 25.0%
! AAAAATAACCCTTGATCAAT+GGG - chr2_4:60793751-60793770 None:intergenic 25.0%
!! GAATTAACGATTTTTGTCAT+AGG - chr2_4:60793911-60793930 None:intergenic 25.0%
GCTTAAAGCATATTCTTCTT+TGG + chr2_4:60790568-60790587 Msa0339590:five_prime_UTR 30.0%
TTGCATTATAAGAGCCTATT+AGG + chr2_4:60790597-60790616 Msa0339590:five_prime_UTR 30.0%
! TCTAGAACAACAAGAAGAAA+AGG + chr2_4:60790657-60790676 Msa0339590:five_prime_UTR 30.0%
CTAGAACAACAAGAAGAAAA+GGG + chr2_4:60790658-60790677 Msa0339590:five_prime_UTR 30.0%
TTTCACTGTGAAATTCATGT+TGG + chr2_4:60790923-60790942 Msa0339590:intron 30.0%
AACTAACTAGATCGGAAAAA+GGG - chr2_4:60790967-60790986 None:intergenic 30.0%
AAACTAACTAGATCGGAAAA+AGG - chr2_4:60790968-60790987 None:intergenic 30.0%
CATAGATCCTTATTCCATAT+AGG + chr2_4:60791081-60791100 Msa0339590:intron 30.0%
AGCATATCCTATATGGAATA+AGG - chr2_4:60791091-60791110 None:intergenic 30.0%
TTGTTTGAGCATATCCTATA+TGG - chr2_4:60791098-60791117 None:intergenic 30.0%
AAAAGAAAAACAGTTCCACA+AGG - chr2_4:60791172-60791191 None:intergenic 30.0%
!! GTGGAACTGTTTTTCTTTTT+AGG + chr2_4:60791173-60791192 Msa0339590:intron 30.0%
TTTACTGCTACGTAAATATG+CGG - chr2_4:60791542-60791561 None:intergenic 30.0%
AGGATATTGCAATATCAGTA+AGG - chr2_4:60791757-60791776 None:intergenic 30.0%
TCAGTGGTTTATTAATAGTG+TGG + chr2_4:60792172-60792191 Msa0339590:intron 30.0%
! TGCTGAAATGCTTGTTAATT+TGG + chr2_4:60792259-60792278 Msa0339590:CDS 30.0%
GCTTCTAAATGAAACAAATG+AGG + chr2_4:60792322-60792341 Msa0339590:CDS 30.0%
!!! ACAAACTTTTTTGTTGTCTC+TGG + chr2_4:60792346-60792365 Msa0339590:intron 30.0%
!!! CAAACTTTTTTGTTGTCTCT+GGG + chr2_4:60792347-60792366 Msa0339590:intron 30.0%
GGATGATAAAGCAGTAAAAT+GGG - chr2_4:60792452-60792471 None:intergenic 30.0%
TTTGTCTGCAAGTAAAATTG+AGG - chr2_4:60792770-60792789 None:intergenic 30.0%
!! AAACTGGGATAATGTTGTTT+TGG + chr2_4:60792795-60792814 Msa0339590:CDS 30.0%
AAGAATGAGATGTATCAACA+GGG - chr2_4:60792949-60792968 None:intergenic 30.0%
AAAGAATGAGATGTATCAAC+AGG - chr2_4:60792950-60792969 None:intergenic 30.0%
!!! ATATTGATGGTTTTTTGCCT+AGG + chr2_4:60793032-60793051 Msa0339590:intron 30.0%
ACCAAAAATCCATCAATATC+AGG - chr2_4:60793177-60793196 None:intergenic 30.0%
!!! GATATTGATGGATTTTTGGT+TGG + chr2_4:60793177-60793196 Msa0339590:CDS 30.0%
TAAACACAAGTTCAAAGCAT+TGG - chr2_4:60793348-60793367 None:intergenic 30.0%
ACGTACAGTTAAGTAAAACA+CGG - chr2_4:60793490-60793509 None:intergenic 30.0%
CAAAAATAACCCTTGATCAA+TGG - chr2_4:60793752-60793771 None:intergenic 30.0%
!!! TCAAGGGTTATTTTTGTTCA+TGG + chr2_4:60793756-60793775 Msa0339590:three_prime_UTR 30.0%
AAACAAATATAATAGGGGAG+TGG + chr2_4:60793878-60793897 Msa0339590:three_prime_UTR 30.0%
AACAAATATAATAGGGGAGT+GGG + chr2_4:60793879-60793898 Msa0339590:three_prime_UTR 30.0%
TCTCTTAACAAAAATCGAAG+TGG + chr2_4:60793944-60793963 Msa0339590:three_prime_UTR 30.0%
! TCGTAATGTATAAGTTGAAG+TGG - chr2_4:60793976-60793995 None:intergenic 30.0%
ACTTCAACTTATACATTACG+AGG + chr2_4:60793975-60793994 Msa0339590:three_prime_UTR 30.0%
TTATAAGAGCCTATTAGGAC+AGG + chr2_4:60790602-60790621 Msa0339590:five_prime_UTR 35.0%
GATTACGTTTAATTCTGCCA+TGG - chr2_4:60791020-60791039 None:intergenic 35.0%
GGCAATTATGAATGCTAACA+TGG - chr2_4:60791151-60791170 None:intergenic 35.0%
TAGCATTCATAATTGCCTTG+TGG + chr2_4:60791154-60791173 Msa0339590:intron 35.0%
TTCTGGTGATTCATATTGGT+TGG + chr2_4:60791238-60791257 Msa0339590:intron 35.0%
! TCTGGTGATTCATATTGGTT+GGG + chr2_4:60791239-60791258 Msa0339590:intron 35.0%
ATTCATTATACAGGCATGCA+TGG + chr2_4:60791591-60791610 Msa0339590:intron 35.0%
TTGTCAGCACATGCAAATAT+TGG - chr2_4:60791869-60791888 None:intergenic 35.0%
!! TGTGCTGACAAATGTTTTTC+TGG + chr2_4:60791878-60791897 Msa0339590:intron 35.0%
!!! ATGTTTTTCTGGTTCTACAG+AGG + chr2_4:60791889-60791908 Msa0339590:intron 35.0%
! CGAAGTAGACTTTTCACAAA+GGG - chr2_4:60791944-60791963 None:intergenic 35.0%
GTTCTGACACATGATTCAAT+CGG - chr2_4:60792126-60792145 None:intergenic 35.0%
AATCATGTGTCAGAACTGTT+TGG + chr2_4:60792130-60792149 Msa0339590:intron 35.0%
! TCTTTCTCGTTTTGTATCAG+TGG + chr2_4:60792156-60792175 Msa0339590:intron 35.0%
GATATTCCTTGGGTTATTCT+TGG + chr2_4:60792281-60792300 Msa0339590:CDS 35.0%
CGGATGATAAAGCAGTAAAA+TGG - chr2_4:60792453-60792472 None:intergenic 35.0%
! TTGCAGACAAAGTATCAAAC+TGG + chr2_4:60792779-60792798 Msa0339590:intron 35.0%
! TGCAGACAAAGTATCAAACT+GGG + chr2_4:60792780-60792799 Msa0339590:intron 35.0%
TCCATAGATAATCCTTACAG+AGG - chr2_4:60793112-60793131 None:intergenic 35.0%
TCTGTAAGGATTATCTATGG+AGG + chr2_4:60793111-60793130 Msa0339590:CDS 35.0%
ATCTATGGAGGTTCTGTAAA+CGG + chr2_4:60793123-60793142 Msa0339590:CDS 35.0%
!!! GCCTGATATTGATGGATTTT+TGG + chr2_4:60793173-60793192 Msa0339590:CDS 35.0%
!!! ATTGATGGATTTTTGGTTGG+CGG + chr2_4:60793180-60793199 Msa0339590:CDS 35.0%
!! TTGATGATGTCAATGAACTC+TGG - chr2_4:60793219-60793238 None:intergenic 35.0%
! ATGTTTTCCCTTTACCTCAT+CGG + chr2_4:60793381-60793400 Msa0339590:three_prime_UTR 35.0%
CGTACAGTTAAGTAAAACAC+GGG - chr2_4:60793489-60793508 None:intergenic 35.0%
TATCATCAGACCTAAGACAA+TGG - chr2_4:60793519-60793538 None:intergenic 35.0%
! CTGATGATACTTGTGTTGTT+TGG + chr2_4:60793530-60793549 Msa0339590:intron 35.0%
TTGGATTGTGTTATGTCTTG+AGG + chr2_4:60793549-60793568 Msa0339590:intron 35.0%
TGTGTTATGTCTTGAGGATT+TGG + chr2_4:60793555-60793574 Msa0339590:three_prime_UTR 35.0%
! TACTACATGCTAGCTCTTTA+TGG - chr2_4:60793798-60793817 None:intergenic 35.0%
CTTATACATTACGAGGTTTC+AGG + chr2_4:60793982-60794001 Msa0339590:three_prime_UTR 35.0%
!! GAGGTTTCAGGTTTAAACTT+GGG + chr2_4:60793994-60794013 Msa0339590:three_prime_UTR 35.0%
ATGGGCAGAAAATTCTTCGT+CGG + chr2_4:60790720-60790739 Msa0339590:CDS 40.0%
AGAGTGAAGAGAGTGAAAAG+AGG - chr2_4:60790774-60790793 None:intergenic 40.0%
CATATGTGTTAGTGTCGTCT+TGG + chr2_4:60791039-60791058 Msa0339590:intron 40.0%
! TCGTCTTGGATCAAAAGTGT+TGG + chr2_4:60791053-60791072 Msa0339590:intron 40.0%
!! GGGTTGATTTTGTTGCAGAA+TGG + chr2_4:60791259-60791278 Msa0339590:intron 40.0%
! GATTTTCTTCACCTCTTCAG+TGG - chr2_4:60791287-60791306 None:intergenic 40.0%
GAAGAGGTGAAGAAAATCGT+TGG + chr2_4:60791289-60791308 Msa0339590:CDS 40.0%
AATCGTTGGTACTTTGAACG+AGG + chr2_4:60791303-60791322 Msa0339590:CDS 40.0%
! ATGTTGTTGGTGAGTCTTAG+TGG + chr2_4:60791344-60791363 Msa0339590:intron 40.0%
AAAGGGAAGGAACACATAAG+GGG - chr2_4:60791927-60791946 None:intergenic 40.0%
CAAAGGGAAGGAACACATAA+GGG - chr2_4:60791928-60791947 None:intergenic 40.0%
ACAAAGGGAAGGAACACATA+AGG - chr2_4:60791929-60791948 None:intergenic 40.0%
! GTAGACTTTTCACAAAGGGA+AGG - chr2_4:60791940-60791959 None:intergenic 40.0%
! GCGAAGTAGACTTTTCACAA+AGG - chr2_4:60791945-60791964 None:intergenic 40.0%
GATAACATACCTAATCTCCC+CGG - chr2_4:60792023-60792042 None:intergenic 40.0%
! TTTTTATCATCACTGCCGCA+AGG + chr2_4:60792082-60792101 Msa0339590:intron 40.0%
! TTTTATCATCACTGCCGCAA+GGG + chr2_4:60792083-60792102 Msa0339590:intron 40.0%
TCATACACAGCTAGCACAAA+GGG - chr2_4:60792389-60792408 None:intergenic 40.0%
TTATCATCCGCTACAGTTTG+TGG + chr2_4:60792463-60792482 Msa0339590:intron 40.0%
TATCATCCGCTACAGTTTGT+GGG + chr2_4:60792464-60792483 Msa0339590:intron 40.0%
TTGCATATGCACTCTCACTA+GGG + chr2_4:60792495-60792514 Msa0339590:CDS 40.0%
AGGAAAGTCATTGCTTGCAT+TGG + chr2_4:60792518-60792537 Msa0339590:CDS 40.0%
GGAAAGTCATTGCTTGCATT+GGG + chr2_4:60792519-60792538 Msa0339590:CDS 40.0%
GAAAGTCATTGCTTGCATTG+GGG + chr2_4:60792520-60792539 Msa0339590:CDS 40.0%
TGTTGTTGCTGAGCAAACAA+AGG + chr2_4:60792580-60792599 Msa0339590:CDS 40.0%
!!! TTCATCACTTGCTGTTTTGC+TGG + chr2_4:60792725-60792744 Msa0339590:intron 40.0%
! TTTTGGCATATGAGCCAGTT+TGG + chr2_4:60792812-60792831 Msa0339590:CDS 40.0%
TTTGGCATATGAGCCAGTTT+GGG + chr2_4:60792813-60792832 Msa0339590:CDS 40.0%
!! TCCATGCTGCTTTAAGGAAA+TGG + chr2_4:60793055-60793074 Msa0339590:CDS 40.0%
!! CCATGCTGCTTTAAGGAAAT+GGG + chr2_4:60793056-60793075 Msa0339590:CDS 40.0%
GCCTCTGTAAGGATTATCTA+TGG + chr2_4:60793108-60793127 Msa0339590:CDS 40.0%
ACTTCAGTTCTTCTTCACTG+TGG - chr2_4:60793247-60793266 None:intergenic 40.0%
TTAAGTAAAACACGGGCGTT+CGG - chr2_4:60793482-60793501 None:intergenic 40.0%
ACGTTTATCGCCATTGTCTT+AGG + chr2_4:60793506-60793525 Msa0339590:three_prime_UTR 40.0%
GAATTGTCGTCATCACAGAA+AGG + chr2_4:60793629-60793648 Msa0339590:three_prime_UTR 40.0%
TCTCTGCTGAACTATGTTCT+AGG + chr2_4:60793654-60793673 Msa0339590:three_prime_UTR 40.0%
CTCTGCTGAACTATGTTCTA+GGG + chr2_4:60793655-60793674 Msa0339590:three_prime_UTR 40.0%
!! CGAGGTTTCAGGTTTAAACT+TGG + chr2_4:60793993-60794012 Msa0339590:three_prime_UTR 40.0%
AATTCTTCGTCGGTGGTAAC+TGG + chr2_4:60790730-60790749 Msa0339590:CDS 45.0%
TCACCAACAACATCTTCTCC+GGG - chr2_4:60791337-60791356 None:intergenic 45.0%
CTCACCAACAACATCTTCTC+CGG - chr2_4:60791338-60791357 None:intergenic 45.0%
GTAAATATGCGGTTCCCTCT+TGG - chr2_4:60791531-60791550 None:intergenic 45.0%
AAGGGAAGGAACACATAAGG+GGG - chr2_4:60791926-60791945 None:intergenic 45.0%
!! AAGCGTTTTGAGCAGCAACA+TGG - chr2_4:60791976-60791995 None:intergenic 45.0%
ATGTTGCTGCTCAAAACGCT+TGG + chr2_4:60791975-60791994 Msa0339590:CDS 45.0%
TGTTGCTGCTCAAAACGCTT+GGG + chr2_4:60791976-60791995 Msa0339590:CDS 45.0%
CATACACAGCTAGCACAAAG+GGG - chr2_4:60792388-60792407 None:intergenic 45.0%
GTCATACACAGCTAGCACAA+AGG - chr2_4:60792390-60792409 None:intergenic 45.0%
TTGTCTCCCACAAACTGTAG+CGG - chr2_4:60792473-60792492 None:intergenic 45.0%
GTTGCATATGCACTCTCACT+AGG + chr2_4:60792494-60792513 Msa0339590:CDS 45.0%
ACTCTTGAACAACGTGAAGC+TGG + chr2_4:60792545-60792564 Msa0339590:CDS 45.0%
GAGCAAACAAAGGCAATTGC+AGG + chr2_4:60792590-60792609 Msa0339590:CDS 45.0%
CCCATTTCCTTAAAGCAGCA+TGG - chr2_4:60793059-60793078 None:intergenic 45.0%
! AAATGGGTTCACGACAACGT+AGG + chr2_4:60793072-60793091 Msa0339590:CDS 45.0%
TATGGAGGTTCTGTAAACGG+AGG + chr2_4:60793126-60793145 Msa0339590:CDS 45.0%
CGGAGGAAACTCCAAAGAAT+TGG + chr2_4:60793143-60793162 Msa0339590:CDS 45.0%
AGGCTGAGATGCCAATTCTT+TGG - chr2_4:60793157-60793176 None:intergenic 45.0%
GCATCTCAGCCTGATATTGA+TGG + chr2_4:60793165-60793184 Msa0339590:CDS 45.0%
! GTCAATGAACTCTGGCTTCA+AGG - chr2_4:60793211-60793230 None:intergenic 45.0%
CAGAAATTTCCGAAGCAGCT+TGG - chr2_4:60793298-60793317 None:intergenic 45.0%
! TTTTCCCTTTACCTCATCGG+TGG + chr2_4:60793384-60793403 Msa0339590:three_prime_UTR 45.0%
TTTCCCTTTACCTCATCGGT+GGG + chr2_4:60793385-60793404 Msa0339590:three_prime_UTR 45.0%
GTCGTGACTGCCATAACATT+TGG + chr2_4:60793444-60793463 Msa0339590:three_prime_UTR 45.0%
GAATCAATTTGGCGACAGAC+CGG + chr2_4:60793705-60793724 Msa0339590:three_prime_UTR 45.0%
AGGCTGAACCCATTGATCAA+GGG + chr2_4:60793740-60793759 Msa0339590:three_prime_UTR 45.0%
!! ATATTTAGTTAAAAATTTAA+GGG - chr2_4:60791699-60791718 None:intergenic 5.0%
!! TTATTATAAACAAATATAAT+AGG + chr2_4:60793871-60793890 Msa0339590:three_prime_UTR 5.0%
!! TATTATAAACAAATATAATA+GGG + chr2_4:60793872-60793891 Msa0339590:three_prime_UTR 5.0%
AGAGCCTATTAGGACAGGAC+AGG + chr2_4:60790607-60790626 Msa0339590:five_prime_UTR 50.0%
CTGTCCTGTCCTGTCCTAAT+AGG - chr2_4:60790614-60790633 None:intergenic 50.0%
GGCAGAAAATTCTTCGTCGG+TGG + chr2_4:60790723-60790742 Msa0339590:CDS 50.0%
ACACGTACACGTTGACACCA+TGG + chr2_4:60791000-60791019 Msa0339590:intron 50.0%
GCAGAATGGAACCACTGAAG+AGG + chr2_4:60791273-60791292 Msa0339590:intron 50.0%
TTGAACGAGGCTCAAGTTCC+CGG + chr2_4:60791316-60791335 Msa0339590:CDS 50.0%
GTTCCCGGAGAAGATGTTGT+TGG + chr2_4:60791331-60791350 Msa0339590:CDS 50.0%
GGCAACTGCAGTGAATTCAG+TGG - chr2_4:60791736-60791755 None:intergenic 50.0%
AGGGAAGGAACACATAAGGG+GGG - chr2_4:60791925-60791944 None:intergenic 50.0%
CAAAACGCTTGGGTTCGCAA+AGG + chr2_4:60791986-60792005 Msa0339590:CDS 50.0%
CGAAAAGACCGAAACCCTTG+CGG - chr2_4:60792100-60792119 None:intergenic 50.0%
GAGTGGCCAAGAATAACCCA+AGG - chr2_4:60792290-60792309 None:intergenic 50.0%
CATATGCACTCTCACTAGGG+AGG + chr2_4:60792498-60792517 Msa0339590:CDS 50.0%
TATGAGCCAGTTTGGGCCAT+TGG + chr2_4:60792820-60792839 Msa0339590:CDS 50.0%
ATGAGCCAGTTTGGGCCATT+GGG + chr2_4:60792821-60792840 Msa0339590:CDS 50.0%
AGCAACCTTTCCAGTCCCAA+TGG - chr2_4:60792839-60792858 None:intergenic 50.0%
AAAGGTTGCTTCTCCTGCTC+AGG + chr2_4:60792849-60792868 Msa0339590:CDS 50.0%
CCTTAAAGCAGCATGGACCT+AGG - chr2_4:60793052-60793071 None:intergenic 50.0%
!! CCTAGGTCCATGCTGCTTTA+AGG + chr2_4:60793049-60793068 Msa0339590:intron 50.0%
CTGAAGTTGCTGCCTCTGTA+AGG + chr2_4:60793097-60793116 Msa0339590:CDS 50.0%
!! TGCTGCTTTCCAAGCTGCTT+CGG + chr2_4:60793286-60793305 Msa0339590:three_prime_UTR 50.0%
CAGGCTGAACCCATTGATCA+AGG + chr2_4:60793739-60793758 Msa0339590:three_prime_UTR 50.0%
AACGCTTGGGTTCGCAAAGG+TGG + chr2_4:60791989-60792008 Msa0339590:CDS 55.0%
CGCAAAGGTGGTGCTTACAC+CGG + chr2_4:60792001-60792020 Msa0339590:CDS 55.0%
GCAAAGGTGGTGCTTACACC+GGG + chr2_4:60792002-60792021 Msa0339590:CDS 55.0%
CAAAGGTGGTGCTTACACCG+GGG + chr2_4:60792003-60792022 Msa0339590:CDS 55.0%
GTGCTTACACCGGGGAGATT+AGG + chr2_4:60792011-60792030 Msa0339590:CDS 55.0%
CATCACTGCCGCAAGGGTTT+CGG + chr2_4:60792089-60792108 Msa0339590:intron 55.0%
TTCTTGGCCACTCTGAGCGA+AGG + chr2_4:60792297-60792316 Msa0339590:CDS 55.0%
TTGGGCCATTGGGACTGGAA+AGG + chr2_4:60792831-60792850 Msa0339590:CDS 55.0%
TTCTCCTGCTCAGGCTCAAG+AGG + chr2_4:60792858-60792877 Msa0339590:CDS 55.0%
ACTACCTCTTGAGCCTGAGC+AGG - chr2_4:60792865-60792884 None:intergenic 55.0%
TCGCCCACCGATGAGGTAAA+GGG - chr2_4:60793391-60793410 None:intergenic 55.0%
ATCGCCCACCGATGAGGTAA+AGG - chr2_4:60793392-60793411 None:intergenic 55.0%
CAATTCATCGCCCACCGATG+AGG - chr2_4:60793398-60793417 None:intergenic 55.0%
CTCATCGGTGGGCGATGAAT+TGG + chr2_4:60793396-60793415 Msa0339590:three_prime_UTR 55.0%
CAATTTGGCGACAGACCGGT+TGG + chr2_4:60793709-60793728 Msa0339590:three_prime_UTR 55.0%
TCAGCCTGTCCGTAACCAAC+CGG - chr2_4:60793727-60793746 None:intergenic 55.0%
GAAGCTGCCTTCGCTCAGAG+TGG - chr2_4:60792307-60792326 None:intergenic 60.0%
CCAGTCCCAATGGCCCAAAC+TGG - chr2_4:60792829-60792848 None:intergenic 60.0%
CCAGTTTGGGCCATTGGGAC+TGG + chr2_4:60792826-60792845 Msa0339590:CDS 60.0%
! GGCGACAGACCGGTTGGTTA+CGG + chr2_4:60793715-60793734 Msa0339590:three_prime_UTR 60.0%
! CAGACCGGTTGGTTACGGAC+AGG + chr2_4:60793720-60793739 Msa0339590:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 60790528 60794027 60790528 ID=Msa0339590;Name=Msa0339590
chr2_4 mRNA 60790528 60794027 60790528 ID=Msa0339590-mRNA-1;Parent=Msa0339590;Name=Msa0339590-mRNA-1;_AED=0.08;_eAED=0.08;_QI=192|1|1|1|0.85|0.75|8|739|253
chr2_4 exon 60790528 60790758 60790528 ID=Msa0339590-mRNA-1:exon:18942;Parent=Msa0339590-mRNA-1
chr2_4 exon 60791277 60791352 60791277 ID=Msa0339590-mRNA-1:exon:18943;Parent=Msa0339590-mRNA-1
chr2_4 exon 60791909 60792032 60791909 ID=Msa0339590-mRNA-1:exon:18944;Parent=Msa0339590-mRNA-1
chr2_4 exon 60792259 60792343 60792259 ID=Msa0339590-mRNA-1:exon:18945;Parent=Msa0339590-mRNA-1
chr2_4 exon 60792479 60792611 60792479 ID=Msa0339590-mRNA-1:exon:18946;Parent=Msa0339590-mRNA-1
chr2_4 exon 60792785 60792879 60792785 ID=Msa0339590-mRNA-1:exon:18947;Parent=Msa0339590-mRNA-1
chr2_4 exon 60793054 60793527 60793054 ID=Msa0339590-mRNA-1:exon:18948;Parent=Msa0339590-mRNA-1
chr2_4 exon 60793553 60794027 60793553 ID=Msa0339590-mRNA-1:exon:18949;Parent=Msa0339590-mRNA-1
chr2_4 five_prime_UTR 60790528 60790719 60790528 ID=Msa0339590-mRNA-1:five_prime_utr;Parent=Msa0339590-mRNA-1
chr2_4 CDS 60790720 60790758 60790720 ID=Msa0339590-mRNA-1:cds;Parent=Msa0339590-mRNA-1
chr2_4 CDS 60791277 60791352 60791277 ID=Msa0339590-mRNA-1:cds;Parent=Msa0339590-mRNA-1
chr2_4 CDS 60791909 60792032 60791909 ID=Msa0339590-mRNA-1:cds;Parent=Msa0339590-mRNA-1
chr2_4 CDS 60792259 60792343 60792259 ID=Msa0339590-mRNA-1:cds;Parent=Msa0339590-mRNA-1
chr2_4 CDS 60792479 60792611 60792479 ID=Msa0339590-mRNA-1:cds;Parent=Msa0339590-mRNA-1
chr2_4 CDS 60792785 60792879 60792785 ID=Msa0339590-mRNA-1:cds;Parent=Msa0339590-mRNA-1
chr2_4 CDS 60793054 60793263 60793054 ID=Msa0339590-mRNA-1:cds;Parent=Msa0339590-mRNA-1
chr2_4 three_prime_UTR 60793264 60793527 60793264 ID=Msa0339590-mRNA-1:three_prime_utr;Parent=Msa0339590-mRNA-1
chr2_4 three_prime_UTR 60793553 60794027 60793553 ID=Msa0339590-mRNA-1:three_prime_utr;Parent=Msa0339590-mRNA-1
Gene Sequence

>Msa0339590

ATGGGCAGAAAATTCTTCGTCGGTGGTAACTGGAAATGCAATGGAACCACTGAAGAGGTGAAGAAAATCGTTGGTACTTTGAACGAGGCTCAAGTTCCCGGAGAAGATGTTGTTGAGGTTGTTGTGAGCCCCCCTTATGTGTTCCTTCCCTTTGTGAAAAGTCTACTTCGCTCTGACTTCCATGTTGCTGCTCAAAACGCTTGGGTTCGCAAAGGTGGTGCTTACACCGGGGAGATTAGTGCTGAAATGCTTGTTAATTTGGATATTCCTTGGGTTATTCTTGGCCACTCTGAGCGAAGGCAGCTTCTAAATGAAACAAATGAGTTTGTGGGAGACAAAGTTGCATATGCACTCTCACTAGGGAGGAAAGTCATTGCTTGCATTGGGGAGACTCTTGAACAACGTGAAGCTGGTACTACAATAGCTGTTGTTGCTGAGCAAACAAAGGCAATTGCAGACAAAGTATCAAACTGGGATAATGTTGTTTTGGCATATGAGCCAGTTTGGGCCATTGGGACTGGAAAGGTTGCTTCTCCTGCTCAGGCTCAAGAGGTCCATGCTGCTTTAAGGAAATGGGTTCACGACAACGTAGGTGCTGAAGTTGCTGCCTCTGTAAGGATTATCTATGGAGGTTCTGTAAACGGAGGAAACTCCAAAGAATTGGCATCTCAGCCTGATATTGATGGATTTTTGGTTGGCGGTGCTTCCTTGAAGCCAGAGTTCATTGACATCATCAACTCTGCCACAGTGAAGAAGAACTGA
Protein sequence

>Msa0339590

MGRKFFVGGNWKCNGTTEEVKKIVGTLNEAQVPGEDVVEVVVSPPYVFLPFVKSLLRSDFHVAAQNAWVRKGGAYTGEISAEMLVNLDIPWVILGHSERRQLLNETNEFVGDKVAYALSLGRKVIACIGETLEQREAGTTIAVVAEQTKAIADKVSNWDNVVLAYEPVWAIGTGKVASPAQAQEVHAALRKWVHDNVGAEVAASVRIIYGGSVNGGNSKELASQPDIDGFLVGGASLKPEFIDIINSATVKKN*