AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0222930

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0222930 MtrunA17_Chr2g0328161 100.000 253 0 0 1 253 1 253 0.0 513
Msa0222930 MtrunA17_Chr7g0264951 63.200 250 89 2 4 252 62 309 3.99e-110 320
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0222930 AT3G55440.1 81.423 253 47 0 1 253 1 253 4.40e-154 429
Msa0222930 AT2G21170.3 63.600 250 88 2 4 252 65 312 1.79e-109 318
Msa0222930 AT2G21170.1 63.600 250 88 2 4 252 65 312 1.79e-109 318
Msa0222930 AT2G21170.2 62.083 240 88 2 14 252 66 303 6.90e-100 293

Find 117 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAGGTCCATGCTGCTTTA+AGG 0.106869 2_1:+72347302 Msa0222930:intron
TGCTGAAATGCTTGTTAATT+TGG 0.126755 2_1:+72346512 Msa0222930:CDS
TACTACATGCTAGCTCTTTA+TGG 0.167583 2_1:-72348024 None:intergenic
CTTATACATTACGAGGTTTC+AGG 0.215165 2_1:+72348211 Msa0222930:three_prime_UTR
TTATTATAAACAAATATAAT+AGG 0.225661 2_1:+72348100 Msa0222930:three_prime_UTR
GATATTCCTTGGGTTATTCT+TGG 0.231384 2_1:+72346534 Msa0222930:CDS
TTGCATTATAAGAGCCTATT+AGG 0.248380 2_1:+72344884 Msa0222930:five_prime_UTR
GCTTAAAGCATATTCTTCTT+TGG 0.258020 2_1:+72344855 Msa0222930:five_prime_UTR
ATAAGTGTATTGAATCAATT+TGG 0.259889 2_1:+72347923 Msa0222930:three_prime_UTR
AAACTGGGATAATGTTGTTT+TGG 0.298614 2_1:+72347048 Msa0222930:CDS
TCTCTGCTGAACTATGTTCT+AGG 0.305712 2_1:+72347883 Msa0222930:three_prime_UTR
TGTGTTATGTCTTGAGGATT+TGG 0.307049 2_1:+72347783 Msa0222930:three_prime_UTR
TCCATGCTGCTTTAAGGAAA+TGG 0.316327 2_1:+72347308 Msa0222930:CDS
CTCATCGGTGGGCGATGAAT+TGG 0.317824 2_1:+72347649 Msa0222930:three_prime_UTR
AGGCTGAGATGCCAATTCTT+TGG 0.323629 2_1:-72347407 None:intergenic
AATTCTTCGTCGGTGGTAAC+TGG 0.328227 2_1:+72345009 Msa0222930:CDS
TATTATAAACAAATATAATA+GGG 0.346860 2_1:+72348101 Msa0222930:three_prime_UTR
TGTTAATTTGGATATTCCTT+GGG 0.361669 2_1:+72346524 Msa0222930:CDS
GCATCTCAGCCTGATATTGA+TGG 0.369973 2_1:+72347418 Msa0222930:CDS
TTGCAGACAAAGTATCAAAC+TGG 0.370984 2_1:+72347032 Msa0222930:intron
TTGTTAATTTGGATATTCCT+TGG 0.377981 2_1:+72346523 Msa0222930:CDS
GGAAAGTCATTGCTTGCATT+GGG 0.383771 2_1:+72346772 Msa0222930:CDS
TTTGGCATATGAGCCAGTTT+GGG 0.383886 2_1:+72347066 Msa0222930:CDS
GCCTCTGTAAGGATTATCTA+TGG 0.388025 2_1:+72347361 Msa0222930:CDS
CCAGTTTGGGCCATTGGGAC+TGG 0.396885 2_1:+72347079 Msa0222930:CDS
AGTGACTGTCCTGTCCTAAT+AGG 0.402245 2_1:-72344898 None:intergenic
CGGAGGAAACTCCAAAGAAT+TGG 0.404230 2_1:+72347396 Msa0222930:CDS
CAAAGGGAAGGAACACATAA+GGG 0.405048 2_1:-72346178 None:intergenic
TATGAGCCAGTTTGGGCCAT+TGG 0.406432 2_1:+72347073 Msa0222930:CDS
TGATGACGACAATTCACTTC+CGG 0.406771 2_1:-72347850 None:intergenic
GTCAATGAACTCTGGCTTCA+AGG 0.409151 2_1:-72347461 None:intergenic
CGAGGTTTCAGGTTTAAACT+TGG 0.409231 2_1:+72348222 Msa0222930:three_prime_UTR
GTCGTGACTGCCATAACATT+TGG 0.413861 2_1:+72347697 Msa0222930:three_prime_UTR
GTGCTTACACCGGGGAGATT+AGG 0.414986 2_1:+72346264 Msa0222930:CDS
ATCTATGGAGGTTCTGTAAA+CGG 0.416225 2_1:+72347376 Msa0222930:CDS
ATGAGCCAGTTTGGGCCATT+GGG 0.421695 2_1:+72347074 Msa0222930:CDS
TAGTCTAGAACAAGAAGAAA+AGG 0.426640 2_1:+72344936 Msa0222930:five_prime_UTR
TTGAACGAGGCTCAAGTTCC+CGG 0.427132 2_1:+72345565 Msa0222930:CDS
TTATTACTAACCAAATGTTA+TGG 0.428730 2_1:-72347707 None:intergenic
TTAAGTAAAACACGGGCGTT+CGG 0.431142 2_1:-72347732 None:intergenic
CCAGTCCCAATGGCCCAAAC+TGG 0.435261 2_1:-72347079 None:intergenic
CCATGCTGCTTTAAGGAAAT+GGG 0.440149 2_1:+72347309 Msa0222930:CDS
AAAGGTTGCTTCTCCTGCTC+AGG 0.446019 2_1:+72347102 Msa0222930:CDS
TGCTGCTTTCCAAGCTGCTT+CGG 0.446387 2_1:+72347539 Msa0222930:three_prime_UTR
GGCGACAGACCGGTTGGTTA+CGG 0.448337 2_1:+72347944 Msa0222930:three_prime_UTR
GAGCAAACAAAGGCAATTGC+AGG 0.449292 2_1:+72346843 Msa0222930:CDS
AGGAAAGTCATTGCTTGCAT+TGG 0.454029 2_1:+72346771 Msa0222930:CDS
TGTTGCTGCTCAAAACGCTT+GGG 0.460320 2_1:+72346229 Msa0222930:CDS
CAGAAATTTCCGAAGCAGCT+TGG 0.467398 2_1:-72347548 None:intergenic
CTGAAGTTGCTGCCTCTGTA+AGG 0.468253 2_1:+72347350 Msa0222930:CDS
TATCATCCGCTACAGTTTGT+GGG 0.468679 2_1:+72346717 Msa0222930:intron
ATCGCCCACCGATGAGGTAA+AGG 0.469952 2_1:-72347642 None:intergenic
CTCACCAACAACATCTTCTC+CGG 0.472241 2_1:-72345584 None:intergenic
GCAAAGGTGGTGCTTACACC+GGG 0.472783 2_1:+72346255 Msa0222930:CDS
AACAAATATAATAGGGGAGT+GGG 0.482103 2_1:+72348108 Msa0222930:three_prime_UTR
ATGTTGCTGCTCAAAACGCT+TGG 0.482349 2_1:+72346228 Msa0222930:CDS
TTAGATTGTGTTATGTCTTG+AGG 0.492241 2_1:+72347777 Msa0222930:three_prime_UTR
CAGGCTGAACCCATTGATCA+AGG 0.495327 2_1:+72347968 Msa0222930:three_prime_UTR
GTTGCATATGCACTCTCACT+AGG 0.495343 2_1:+72346747 Msa0222930:CDS
CGCAAAGGTGGTGCTTACAC+CGG 0.498016 2_1:+72346254 Msa0222930:CDS
TCAGCCTGTCCGTAACCAAC+CGG 0.512292 2_1:-72347953 None:intergenic
TCGCCCACCGATGAGGTAAA+GGG 0.517509 2_1:-72347641 None:intergenic
AAACAAATATAATAGGGGAG+TGG 0.521187 2_1:+72348107 Msa0222930:three_prime_UTR
AACGCTTGGGTTCGCAAAGG+TGG 0.525911 2_1:+72346242 Msa0222930:CDS
ACTACCTCTTGAGCCTGAGC+AGG 0.528540 2_1:-72347115 None:intergenic
ACTCTTGAACAACGTGAAGC+TGG 0.531573 2_1:+72346798 Msa0222930:CDS
ACAAAGGGAAGGAACACATA+AGG 0.532183 2_1:-72346179 None:intergenic
TTCTCCTGCTCAGGCTCAAG+AGG 0.539203 2_1:+72347111 Msa0222930:CDS
GTTCCCGGAGAAGATGTTGT+TGG 0.540063 2_1:+72345580 Msa0222930:CDS
CTCTGCTGAACTATGTTCTA+GGG 0.543887 2_1:+72347884 Msa0222930:three_prime_UTR
AGTCTAGAACAAGAAGAAAA+GGG 0.544466 2_1:+72344937 Msa0222930:five_prime_UTR
TAAACACAAGTTCAAAGCAT+TGG 0.547440 2_1:-72347598 None:intergenic
GAGGTTTCAGGTTTAAACTT+GGG 0.552233 2_1:+72348223 Msa0222930:three_prime_UTR
ATTATAAACAAATATAATAG+GGG 0.554042 2_1:+72348102 Msa0222930:three_prime_UTR
TTGGGCCATTGGGACTGGAA+AGG 0.554351 2_1:+72347084 Msa0222930:CDS
CCCATTTCCTTAAAGCAGCA+TGG 0.555610 2_1:-72347309 None:intergenic
GAATCAATTTGGCGACAGAC+CGG 0.559054 2_1:+72347934 Msa0222930:three_prime_UTR
CAATTTGGCGACAGACCGGT+TGG 0.561018 2_1:+72347938 Msa0222930:three_prime_UTR
TCACCAACAACATCTTCTCC+GGG 0.561852 2_1:-72345583 None:intergenic
TTCTTGGCCACTCTGAGCGA+AGG 0.565910 2_1:+72346550 Msa0222930:CDS
TTATAAGAGCCTATTAGGAC+AGG 0.566780 2_1:+72344889 Msa0222930:five_prime_UTR
TTTCCCTTTACCTCATCGGT+GGG 0.569814 2_1:+72347638 Msa0222930:three_prime_UTR
CATAACACAATCTAAGACAA+TGG 0.575187 2_1:-72347769 None:intergenic
TCGTAATGTATAAGTTGAAG+TGG 0.578365 2_1:-72348202 None:intergenic
TTGATGATGTCAATGAACTC+TGG 0.578436 2_1:-72347469 None:intergenic
GCTTCTAAATGAAACAAATG+AGG 0.602961 2_1:+72346575 Msa0222930:CDS
ACTTCAACTTATACATTACG+AGG 0.604440 2_1:+72348204 Msa0222930:three_prime_UTR
GAAGAGGTGAAGAAAATCGT+TGG 0.610298 2_1:+72345538 Msa0222930:CDS
AGCAACCTTTCCAGTCCCAA+TGG 0.611585 2_1:-72347089 None:intergenic
CAATTCATCGCCCACCGATG+AGG 0.616929 2_1:-72347648 None:intergenic
ATGGGCAGAAAATTCTTCGT+CGG 0.618039 2_1:+72344999 Msa0222930:CDS
TTGCATATGCACTCTCACTA+GGG 0.619513 2_1:+72346748 Msa0222930:CDS
GAATTGTCGTCATCACAGAA+AGG 0.621483 2_1:+72347858 Msa0222930:three_prime_UTR
AAAGGGAAGGAACACATAAG+GGG 0.623658 2_1:-72346177 None:intergenic
GGCAGAAAATTCTTCGTCGG+TGG 0.629014 2_1:+72345002 Msa0222930:CDS
GAAAGTCATTGCTTGCATTG+GGG 0.633688 2_1:+72346773 Msa0222930:CDS
AGGCTGAACCCATTGATCAA+GGG 0.634403 2_1:+72347969 Msa0222930:three_prime_UTR
AAGGGAAGGAACACATAAGG+GGG 0.637952 2_1:-72346176 None:intergenic
TGCAGACAAAGTATCAAACT+GGG 0.639857 2_1:+72347033 Msa0222930:intron
CAGACCGGTTGGTTACGGAC+AGG 0.641537 2_1:+72347949 Msa0222930:three_prime_UTR
CAAAACGCTTGGGTTCGCAA+AGG 0.648775 2_1:+72346239 Msa0222930:CDS
TGTTGTTGCTGAGCAAACAA+AGG 0.653424 2_1:+72346833 Msa0222930:CDS
TCTGTAAGGATTATCTATGG+AGG 0.664957 2_1:+72347364 Msa0222930:CDS
ACGTACAGTTAAGTAAAACA+CGG 0.667629 2_1:-72347740 None:intergenic
TCCATAGATAATCCTTACAG+AGG 0.672902 2_1:-72347362 None:intergenic
GCAGAATGGAACCACTGAAG+AGG 0.675685 2_1:+72345522 Msa0222930:intron
CATATGCACTCTCACTAGGG+AGG 0.678402 2_1:+72346751 Msa0222930:CDS
GAAGCTGCCTTCGCTCAGAG+TGG 0.679228 2_1:-72346557 None:intergenic
GAGTGGCCAAGAATAACCCA+AGG 0.679606 2_1:-72346540 None:intergenic
CGTACAGTTAAGTAAAACAC+GGG 0.684223 2_1:-72347739 None:intergenic
GATAACATACCTAATCTCCC+CGG 0.689404 2_1:-72346273 None:intergenic
TATGGAGGTTCTGTAAACGG+AGG 0.700353 2_1:+72347379 Msa0222930:CDS
AATCGTTGGTACTTTGAACG+AGG 0.707863 2_1:+72345552 Msa0222930:CDS
AAATGGGTTCACGACAACGT+AGG 0.725939 2_1:+72347325 Msa0222930:CDS
ACTTCAGTTCTTCTTCACTG+TGG 0.744514 2_1:-72347497 None:intergenic
AGGGAAGGAACACATAAGGG+GGG 0.750761 2_1:-72346175 None:intergenic
CAAAGGTGGTGCTTACACCG+GGG 0.762885 2_1:+72346256 Msa0222930:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATATTTAGTTAAAAATTTA+AGG - chr2_1:72345950-72345969 None:intergenic 10.0%
!!! AAATTTTAAAAACAGTATAT+AGG - chr2_1:72347145-72347164 None:intergenic 10.0%
!! ATTATAAACAAATATAATAG+GGG + chr2_1:72348102-72348121 Msa0222930:three_prime_UTR 10.0%
!! AGAGATACAAAAAAAAAAAA+AGG - chr2_1:72346027-72346046 None:intergenic 15.0%
!!! TCAATAAAAATAATTTTCGT+TGG - chr2_1:72346080-72346099 None:intergenic 15.0%
!! AACTATGTTAAAAATCAGAT+CGG + chr2_1:72345122-72345141 Msa0222930:intron 20.0%
!!! TGATTTTCTCAAAATATTAC+CGG - chr2_1:72345252-72345271 None:intergenic 20.0%
!! GTATATAGACTATATATTTC+TGG + chr2_1:72345470-72345489 Msa0222930:intron 20.0%
!!! AGTAAATTTCAGTATTGATT+TGG + chr2_1:72345694-72345713 Msa0222930:intron 20.0%
!!! ATAAGAAATTTTTTTCCAAG+AGG + chr2_1:72345762-72345781 Msa0222930:intron 20.0%
!!! TAAGAAATTTTTTTCCAAGA+GGG + chr2_1:72345763-72345782 Msa0222930:intron 20.0%
!!! TCTCATTTTAATAATGCAAA+GGG - chr2_1:72345816-72345835 None:intergenic 20.0%
!! TTAACTAAATATCAAGTTTC+TGG + chr2_1:72345957-72345976 Msa0222930:intron 20.0%
!! TGGTGAAATAAATCAAATAA+TGG - chr2_1:72346965-72346984 None:intergenic 20.0%
!! TTATTACTAACCAAATGTTA+TGG - chr2_1:72347710-72347729 None:intergenic 20.0%
!! ATAAGTGTATTGAATCAATT+TGG + chr2_1:72347923-72347942 Msa0222930:three_prime_UTR 20.0%
! AATCATCGAAATCAAAAACA+TGG + chr2_1:72344980-72344999 Msa0222930:exon 25.0%
! ATCATCGAAATCAAAAACAT+GGG + chr2_1:72344981-72345000 Msa0222930:exon 25.0%
! CAACTAAAAACTAACTAGAT+CGG - chr2_1:72345207-72345226 None:intergenic 25.0%
!!! ATATTTCTGGTGATTCATAT+TGG + chr2_1:72345483-72345502 Msa0222930:intron 25.0%
!!! GTCTCATTTTAATAATGCAA+AGG - chr2_1:72345817-72345836 None:intergenic 25.0%
! GAGACTTAAATTCATTATAC+AGG + chr2_1:72345832-72345851 Msa0222930:intron 25.0%
!! ACGAAATTTGCTTATCTATT+TGG + chr2_1:72345923-72345942 Msa0222930:intron 25.0%
! AAATAAATCGAAGATCAGTA+CGG - chr2_1:72346298-72346317 None:intergenic 25.0%
!! TACTGATCTTCGATTTATTT+TGG + chr2_1:72346298-72346317 Msa0222930:intron 25.0%
! ACAAACACAAATTAAACGAT+AGG - chr2_1:72346472-72346491 None:intergenic 25.0%
!! TTGTTAATTTGGATATTCCT+TGG + chr2_1:72346523-72346542 Msa0222930:CDS 25.0%
!! TGTTAATTTGGATATTCCTT+GGG + chr2_1:72346524-72346543 Msa0222930:CDS 25.0%
!! TGTTGTCTTGATAATATTGA+TGG + chr2_1:72347272-72347291 Msa0222930:intron 25.0%
!! GGATTTTTGTTAAGTTTCTA+TGG + chr2_1:72347670-72347689 Msa0222930:three_prime_UTR 25.0%
!!! TTTCTTTTTTCTCATCTCAA+GGG - chr2_1:72347810-72347829 None:intergenic 25.0%
!!! ATTTCTTTTTTCTCATCTCA+AGG - chr2_1:72347811-72347830 None:intergenic 25.0%
! AAAAATAACCCTTGATCAAT+GGG - chr2_1:72347980-72347999 None:intergenic 25.0%
!! GAATTAACGATTTTTGTCAT+AGG - chr2_1:72348140-72348159 None:intergenic 25.0%
GCTTAAAGCATATTCTTCTT+TGG + chr2_1:72344855-72344874 Msa0222930:five_prime_UTR 30.0%
TTGCATTATAAGAGCCTATT+AGG + chr2_1:72344884-72344903 Msa0222930:five_prime_UTR 30.0%
! TAGTCTAGAACAAGAAGAAA+AGG + chr2_1:72344936-72344955 Msa0222930:five_prime_UTR 30.0%
! AGTCTAGAACAAGAAGAAAA+GGG + chr2_1:72344937-72344956 Msa0222930:five_prime_UTR 30.0%
TTTCACTGTGAAATTCATGT+TGG + chr2_1:72345155-72345174 Msa0222930:intron 30.0%
AACTAACTAGATCGGAAAAA+GGG - chr2_1:72345199-72345218 None:intergenic 30.0%
AAACTAACTAGATCGGAAAA+AGG - chr2_1:72345200-72345219 None:intergenic 30.0%
CATAGATCCTTATTCCATAT+AGG + chr2_1:72345330-72345349 Msa0222930:intron 30.0%
AGCATATCCTATATGGAATA+AGG - chr2_1:72345340-72345359 None:intergenic 30.0%
TTGTTTGAGCATATCCTATA+TGG - chr2_1:72345347-72345366 None:intergenic 30.0%
AAAAGAAAAACAGTTCCACA+AGG - chr2_1:72345421-72345440 None:intergenic 30.0%
!! GTGGAACTGTTTTTCTTTTT+AGG + chr2_1:72345422-72345441 Msa0222930:intron 30.0%
TTTACTGCTACGTAAATATG+CGG - chr2_1:72345792-72345811 None:intergenic 30.0%
AGGATATTGCAATATCAGTA+AGG - chr2_1:72346007-72346026 None:intergenic 30.0%
TCAGTGGTTTATTAATAGTG+TGG + chr2_1:72346425-72346444 Msa0222930:intron 30.0%
! TGCTGAAATGCTTGTTAATT+TGG + chr2_1:72346512-72346531 Msa0222930:CDS 30.0%
GCTTCTAAATGAAACAAATG+AGG + chr2_1:72346575-72346594 Msa0222930:CDS 30.0%
!!! ACAAACTTTTTTGTTGTCTC+TGG + chr2_1:72346599-72346618 Msa0222930:intron 30.0%
!!! CAAACTTTTTTGTTGTCTCT+GGG + chr2_1:72346600-72346619 Msa0222930:intron 30.0%
GGATGATAAAGCAGTAAAAT+GGG - chr2_1:72346705-72346724 None:intergenic 30.0%
TTTGTCTGCAAGTAAAATTG+AGG - chr2_1:72347023-72347042 None:intergenic 30.0%
!! AAACTGGGATAATGTTGTTT+TGG + chr2_1:72347048-72347067 Msa0222930:CDS 30.0%
AAGAATGAGATGTATCAACA+GGG - chr2_1:72347202-72347221 None:intergenic 30.0%
AAAGAATGAGATGTATCAAC+AGG - chr2_1:72347203-72347222 None:intergenic 30.0%
!!! ATATTGATGGTTTTTTGCCT+AGG + chr2_1:72347285-72347304 Msa0222930:intron 30.0%
ACCAAAAATCCATCAATATC+AGG - chr2_1:72347430-72347449 None:intergenic 30.0%
!!! GATATTGATGGATTTTTGGT+TGG + chr2_1:72347430-72347449 Msa0222930:CDS 30.0%
TAAACACAAGTTCAAAGCAT+TGG - chr2_1:72347601-72347620 None:intergenic 30.0%
ACGTACAGTTAAGTAAAACA+CGG - chr2_1:72347743-72347762 None:intergenic 30.0%
! CATAACACAATCTAAGACAA+TGG - chr2_1:72347772-72347791 None:intergenic 30.0%
TTAGATTGTGTTATGTCTTG+AGG + chr2_1:72347777-72347796 Msa0222930:three_prime_UTR 30.0%
!!! GCAGTTGTTTTTACTAAAAC+CGG + chr2_1:72347831-72347850 Msa0222930:three_prime_UTR 30.0%
CAAAAATAACCCTTGATCAA+TGG - chr2_1:72347981-72348000 None:intergenic 30.0%
!!! TCAAGGGTTATTTTTGTTCA+TGG + chr2_1:72347985-72348004 Msa0222930:three_prime_UTR 30.0%
AAACAAATATAATAGGGGAG+TGG + chr2_1:72348107-72348126 Msa0222930:three_prime_UTR 30.0%
AACAAATATAATAGGGGAGT+GGG + chr2_1:72348108-72348127 Msa0222930:three_prime_UTR 30.0%
TCTCTTAACAAAAATCGAAG+TGG + chr2_1:72348173-72348192 Msa0222930:three_prime_UTR 30.0%
! TCGTAATGTATAAGTTGAAG+TGG - chr2_1:72348205-72348224 None:intergenic 30.0%
ACTTCAACTTATACATTACG+AGG + chr2_1:72348204-72348223 Msa0222930:three_prime_UTR 30.0%
TTATAAGAGCCTATTAGGAC+AGG + chr2_1:72344889-72344908 Msa0222930:five_prime_UTR 35.0%
GGCAATTATGAATGCTAACA+TGG - chr2_1:72345400-72345419 None:intergenic 35.0%
TAGCATTCATAATTGCCTTG+TGG + chr2_1:72345403-72345422 Msa0222930:intron 35.0%
TTCTGGTGATTCATATTGGT+TGG + chr2_1:72345487-72345506 Msa0222930:intron 35.0%
! TCTGGTGATTCATATTGGTT+GGG + chr2_1:72345488-72345507 Msa0222930:intron 35.0%
TTACTGCTACGTAAATATGC+GGG - chr2_1:72345791-72345810 None:intergenic 35.0%
ATTCATTATACAGGCATGCA+TGG + chr2_1:72345841-72345860 Msa0222930:intron 35.0%
TTGTCAGCACATGCAAATAT+TGG - chr2_1:72346122-72346141 None:intergenic 35.0%
!! TGTGCTGACAAATGTTTTTC+TGG + chr2_1:72346131-72346150 Msa0222930:intron 35.0%
!!! ATGTTTTTCTGGTTCTACAG+AGG + chr2_1:72346142-72346161 Msa0222930:intron 35.0%
! CGAAGTAGACTTTTCACAAA+GGG - chr2_1:72346197-72346216 None:intergenic 35.0%
GTTCTGACACATGATTCAAT+CGG - chr2_1:72346379-72346398 None:intergenic 35.0%
AATCATGTGTCAGAACTGTT+TGG + chr2_1:72346383-72346402 Msa0222930:intron 35.0%
! TCTTTCTCGTTTTGTATCAG+TGG + chr2_1:72346409-72346428 Msa0222930:intron 35.0%
GATATTCCTTGGGTTATTCT+TGG + chr2_1:72346534-72346553 Msa0222930:CDS 35.0%
CGGATGATAAAGCAGTAAAA+TGG - chr2_1:72346706-72346725 None:intergenic 35.0%
! TTGCAGACAAAGTATCAAAC+TGG + chr2_1:72347032-72347051 Msa0222930:intron 35.0%
! TGCAGACAAAGTATCAAACT+GGG + chr2_1:72347033-72347052 Msa0222930:intron 35.0%
TCCATAGATAATCCTTACAG+AGG - chr2_1:72347365-72347384 None:intergenic 35.0%
TCTGTAAGGATTATCTATGG+AGG + chr2_1:72347364-72347383 Msa0222930:CDS 35.0%
ATCTATGGAGGTTCTGTAAA+CGG + chr2_1:72347376-72347395 Msa0222930:CDS 35.0%
!!! GCCTGATATTGATGGATTTT+TGG + chr2_1:72347426-72347445 Msa0222930:CDS 35.0%
!!! ATTGATGGATTTTTGGTTGG+CGG + chr2_1:72347433-72347452 Msa0222930:CDS 35.0%
!! TTGATGATGTCAATGAACTC+TGG - chr2_1:72347472-72347491 None:intergenic 35.0%
! ATGTTTTCCCTTTACCTCAT+CGG + chr2_1:72347634-72347653 Msa0222930:three_prime_UTR 35.0%
CGTACAGTTAAGTAAAACAC+GGG - chr2_1:72347742-72347761 None:intergenic 35.0%
TGTGTTATGTCTTGAGGATT+TGG + chr2_1:72347783-72347802 Msa0222930:three_prime_UTR 35.0%
! TACTACATGCTAGCTCTTTA+TGG - chr2_1:72348027-72348046 None:intergenic 35.0%
CTTATACATTACGAGGTTTC+AGG + chr2_1:72348211-72348230 Msa0222930:three_prime_UTR 35.0%
!! GAGGTTTCAGGTTTAAACTT+GGG + chr2_1:72348223-72348242 Msa0222930:three_prime_UTR 35.0%
ATGGGCAGAAAATTCTTCGT+CGG + chr2_1:72344999-72345018 Msa0222930:CDS 40.0%
AGAGTGAAGAGAGTGAAAAG+AGG - chr2_1:72345053-72345072 None:intergenic 40.0%
CATATGTGTTAGTGTCGTCT+TGG + chr2_1:72345288-72345307 Msa0222930:intron 40.0%
! TCGTCTTGGATCAAAAGTGT+TGG + chr2_1:72345302-72345321 Msa0222930:intron 40.0%
! GATTTTCTTCACCTCTTCAG+TGG - chr2_1:72345536-72345555 None:intergenic 40.0%
GAAGAGGTGAAGAAAATCGT+TGG + chr2_1:72345538-72345557 Msa0222930:CDS 40.0%
AATCGTTGGTACTTTGAACG+AGG + chr2_1:72345552-72345571 Msa0222930:CDS 40.0%
! AAGATGTTGTTGGTGAGTCT+TGG + chr2_1:72345590-72345609 Msa0222930:intron 40.0%
AAAGGGAAGGAACACATAAG+GGG - chr2_1:72346180-72346199 None:intergenic 40.0%
CAAAGGGAAGGAACACATAA+GGG - chr2_1:72346181-72346200 None:intergenic 40.0%
ACAAAGGGAAGGAACACATA+AGG - chr2_1:72346182-72346201 None:intergenic 40.0%
! GTAGACTTTTCACAAAGGGA+AGG - chr2_1:72346193-72346212 None:intergenic 40.0%
! GCGAAGTAGACTTTTCACAA+AGG - chr2_1:72346198-72346217 None:intergenic 40.0%
GATAACATACCTAATCTCCC+CGG - chr2_1:72346276-72346295 None:intergenic 40.0%
! TTTTTATCATCACTGCCGCA+AGG + chr2_1:72346335-72346354 Msa0222930:intron 40.0%
! TTTTATCATCACTGCCGCAA+GGG + chr2_1:72346336-72346355 Msa0222930:intron 40.0%
TCATACACAGCTAGCACAAA+GGG - chr2_1:72346642-72346661 None:intergenic 40.0%
TTATCATCCGCTACAGTTTG+TGG + chr2_1:72346716-72346735 Msa0222930:intron 40.0%
TATCATCCGCTACAGTTTGT+GGG + chr2_1:72346717-72346736 Msa0222930:intron 40.0%
TTGCATATGCACTCTCACTA+GGG + chr2_1:72346748-72346767 Msa0222930:CDS 40.0%
AGGAAAGTCATTGCTTGCAT+TGG + chr2_1:72346771-72346790 Msa0222930:CDS 40.0%
GGAAAGTCATTGCTTGCATT+GGG + chr2_1:72346772-72346791 Msa0222930:CDS 40.0%
GAAAGTCATTGCTTGCATTG+GGG + chr2_1:72346773-72346792 Msa0222930:CDS 40.0%
TGTTGTTGCTGAGCAAACAA+AGG + chr2_1:72346833-72346852 Msa0222930:CDS 40.0%
! TTTTGGCATATGAGCCAGTT+TGG + chr2_1:72347065-72347084 Msa0222930:CDS 40.0%
TTTGGCATATGAGCCAGTTT+GGG + chr2_1:72347066-72347085 Msa0222930:CDS 40.0%
!! TCCATGCTGCTTTAAGGAAA+TGG + chr2_1:72347308-72347327 Msa0222930:CDS 40.0%
!! CCATGCTGCTTTAAGGAAAT+GGG + chr2_1:72347309-72347328 Msa0222930:CDS 40.0%
GCCTCTGTAAGGATTATCTA+TGG + chr2_1:72347361-72347380 Msa0222930:CDS 40.0%
ACTTCAGTTCTTCTTCACTG+TGG - chr2_1:72347500-72347519 None:intergenic 40.0%
TTAAGTAAAACACGGGCGTT+CGG - chr2_1:72347735-72347754 None:intergenic 40.0%
! TGATGACGACAATTCACTTC+CGG - chr2_1:72347853-72347872 None:intergenic 40.0%
GAATTGTCGTCATCACAGAA+AGG + chr2_1:72347858-72347877 Msa0222930:three_prime_UTR 40.0%
TCTCTGCTGAACTATGTTCT+AGG + chr2_1:72347883-72347902 Msa0222930:three_prime_UTR 40.0%
CTCTGCTGAACTATGTTCTA+GGG + chr2_1:72347884-72347903 Msa0222930:three_prime_UTR 40.0%
!! CGAGGTTTCAGGTTTAAACT+TGG + chr2_1:72348222-72348241 Msa0222930:three_prime_UTR 40.0%
AGTGACTGTCCTGTCCTAAT+AGG - chr2_1:72344901-72344920 None:intergenic 45.0%
AATTCTTCGTCGGTGGTAAC+TGG + chr2_1:72345009-72345028 Msa0222930:CDS 45.0%
!! GGGCTGATTTTGTTGCAGAA+TGG + chr2_1:72345508-72345527 Msa0222930:intron 45.0%
TCACCAACAACATCTTCTCC+GGG - chr2_1:72345586-72345605 None:intergenic 45.0%
CTCACCAACAACATCTTCTC+CGG - chr2_1:72345587-72345606 None:intergenic 45.0%
! ATGTTGTTGGTGAGTCTTGG+TGG + chr2_1:72345593-72345612 Msa0222930:intron 45.0%
TAAATATGCGGGTTCCCTCT+TGG - chr2_1:72345780-72345799 None:intergenic 45.0%
AAGGGAAGGAACACATAAGG+GGG - chr2_1:72346179-72346198 None:intergenic 45.0%
!! AAGCGTTTTGAGCAGCAACA+TGG - chr2_1:72346229-72346248 None:intergenic 45.0%
ATGTTGCTGCTCAAAACGCT+TGG + chr2_1:72346228-72346247 Msa0222930:CDS 45.0%
TGTTGCTGCTCAAAACGCTT+GGG + chr2_1:72346229-72346248 Msa0222930:CDS 45.0%
CATACACAGCTAGCACAAAG+GGG - chr2_1:72346641-72346660 None:intergenic 45.0%
GTCATACACAGCTAGCACAA+AGG - chr2_1:72346643-72346662 None:intergenic 45.0%
TTGTCTCCCACAAACTGTAG+CGG - chr2_1:72346726-72346745 None:intergenic 45.0%
GTTGCATATGCACTCTCACT+AGG + chr2_1:72346747-72346766 Msa0222930:CDS 45.0%
ACTCTTGAACAACGTGAAGC+TGG + chr2_1:72346798-72346817 Msa0222930:CDS 45.0%
GAGCAAACAAAGGCAATTGC+AGG + chr2_1:72346843-72346862 Msa0222930:CDS 45.0%
!!! TTCACCACTTGCTGTTTTGC+TGG + chr2_1:72346978-72346997 Msa0222930:intron 45.0%
ATTGCCAGCAAAACAGCAAG+TGG - chr2_1:72346985-72347004 None:intergenic 45.0%
CCCATTTCCTTAAAGCAGCA+TGG - chr2_1:72347312-72347331 None:intergenic 45.0%
! AAATGGGTTCACGACAACGT+AGG + chr2_1:72347325-72347344 Msa0222930:CDS 45.0%
TATGGAGGTTCTGTAAACGG+AGG + chr2_1:72347379-72347398 Msa0222930:CDS 45.0%
CGGAGGAAACTCCAAAGAAT+TGG + chr2_1:72347396-72347415 Msa0222930:CDS 45.0%
AGGCTGAGATGCCAATTCTT+TGG - chr2_1:72347410-72347429 None:intergenic 45.0%
GCATCTCAGCCTGATATTGA+TGG + chr2_1:72347418-72347437 Msa0222930:CDS 45.0%
! GTCAATGAACTCTGGCTTCA+AGG - chr2_1:72347464-72347483 None:intergenic 45.0%
CAGAAATTTCCGAAGCAGCT+TGG - chr2_1:72347551-72347570 None:intergenic 45.0%
! TTTTCCCTTTACCTCATCGG+TGG + chr2_1:72347637-72347656 Msa0222930:three_prime_UTR 45.0%
TTTCCCTTTACCTCATCGGT+GGG + chr2_1:72347638-72347657 Msa0222930:three_prime_UTR 45.0%
GTCGTGACTGCCATAACATT+TGG + chr2_1:72347697-72347716 Msa0222930:three_prime_UTR 45.0%
GAATCAATTTGGCGACAGAC+CGG + chr2_1:72347934-72347953 Msa0222930:three_prime_UTR 45.0%
AGGCTGAACCCATTGATCAA+GGG + chr2_1:72347969-72347988 Msa0222930:three_prime_UTR 45.0%
!! ATATTTAGTTAAAAATTTAA+GGG - chr2_1:72345949-72345968 None:intergenic 5.0%
!! TTATTATAAACAAATATAAT+AGG + chr2_1:72348100-72348119 Msa0222930:three_prime_UTR 5.0%
!! TATTATAAACAAATATAATA+GGG + chr2_1:72348101-72348120 Msa0222930:three_prime_UTR 5.0%
GGCAGAAAATTCTTCGTCGG+TGG + chr2_1:72345002-72345021 Msa0222930:CDS 50.0%
TGACACGTACACGTTGACAC+CGG + chr2_1:72345230-72345249 Msa0222930:intron 50.0%
GCAGAATGGAACCACTGAAG+AGG + chr2_1:72345522-72345541 Msa0222930:intron 50.0%
TTGAACGAGGCTCAAGTTCC+CGG + chr2_1:72345565-72345584 Msa0222930:CDS 50.0%
GTTCCCGGAGAAGATGTTGT+TGG + chr2_1:72345580-72345599 Msa0222930:CDS 50.0%
AGGGAAGGAACACATAAGGG+GGG - chr2_1:72346178-72346197 None:intergenic 50.0%
CAAAACGCTTGGGTTCGCAA+AGG + chr2_1:72346239-72346258 Msa0222930:CDS 50.0%
CGAAAAGACCGAAACCCTTG+CGG - chr2_1:72346353-72346372 None:intergenic 50.0%
GAGTGGCCAAGAATAACCCA+AGG - chr2_1:72346543-72346562 None:intergenic 50.0%
CATATGCACTCTCACTAGGG+AGG + chr2_1:72346751-72346770 Msa0222930:CDS 50.0%
TATGAGCCAGTTTGGGCCAT+TGG + chr2_1:72347073-72347092 Msa0222930:CDS 50.0%
ATGAGCCAGTTTGGGCCATT+GGG + chr2_1:72347074-72347093 Msa0222930:CDS 50.0%
AGCAACCTTTCCAGTCCCAA+TGG - chr2_1:72347092-72347111 None:intergenic 50.0%
AAAGGTTGCTTCTCCTGCTC+AGG + chr2_1:72347102-72347121 Msa0222930:CDS 50.0%
CCTTAAAGCAGCATGGACCT+AGG - chr2_1:72347305-72347324 None:intergenic 50.0%
!! CCTAGGTCCATGCTGCTTTA+AGG + chr2_1:72347302-72347321 Msa0222930:intron 50.0%
CTGAAGTTGCTGCCTCTGTA+AGG + chr2_1:72347350-72347369 Msa0222930:CDS 50.0%
!! TGCTGCTTTCCAAGCTGCTT+CGG + chr2_1:72347539-72347558 Msa0222930:three_prime_UTR 50.0%
CAGGCTGAACCCATTGATCA+AGG + chr2_1:72347968-72347987 Msa0222930:three_prime_UTR 50.0%
AACGCTTGGGTTCGCAAAGG+TGG + chr2_1:72346242-72346261 Msa0222930:CDS 55.0%
CGCAAAGGTGGTGCTTACAC+CGG + chr2_1:72346254-72346273 Msa0222930:CDS 55.0%
GCAAAGGTGGTGCTTACACC+GGG + chr2_1:72346255-72346274 Msa0222930:CDS 55.0%
CAAAGGTGGTGCTTACACCG+GGG + chr2_1:72346256-72346275 Msa0222930:CDS 55.0%
GTGCTTACACCGGGGAGATT+AGG + chr2_1:72346264-72346283 Msa0222930:CDS 55.0%
CATCACTGCCGCAAGGGTTT+CGG + chr2_1:72346342-72346361 Msa0222930:intron 55.0%
TTCTTGGCCACTCTGAGCGA+AGG + chr2_1:72346550-72346569 Msa0222930:CDS 55.0%
TTGGGCCATTGGGACTGGAA+AGG + chr2_1:72347084-72347103 Msa0222930:CDS 55.0%
TTCTCCTGCTCAGGCTCAAG+AGG + chr2_1:72347111-72347130 Msa0222930:CDS 55.0%
ACTACCTCTTGAGCCTGAGC+AGG - chr2_1:72347118-72347137 None:intergenic 55.0%
TCGCCCACCGATGAGGTAAA+GGG - chr2_1:72347644-72347663 None:intergenic 55.0%
ATCGCCCACCGATGAGGTAA+AGG - chr2_1:72347645-72347664 None:intergenic 55.0%
CAATTCATCGCCCACCGATG+AGG - chr2_1:72347651-72347670 None:intergenic 55.0%
CTCATCGGTGGGCGATGAAT+TGG + chr2_1:72347649-72347668 Msa0222930:three_prime_UTR 55.0%
CAATTTGGCGACAGACCGGT+TGG + chr2_1:72347938-72347957 Msa0222930:three_prime_UTR 55.0%
TCAGCCTGTCCGTAACCAAC+CGG - chr2_1:72347956-72347975 None:intergenic 55.0%
GAAGCTGCCTTCGCTCAGAG+TGG - chr2_1:72346560-72346579 None:intergenic 60.0%
CCAGTCCCAATGGCCCAAAC+TGG - chr2_1:72347082-72347101 None:intergenic 60.0%
CCAGTTTGGGCCATTGGGAC+TGG + chr2_1:72347079-72347098 Msa0222930:CDS 60.0%
! GGCGACAGACCGGTTGGTTA+CGG + chr2_1:72347944-72347963 Msa0222930:three_prime_UTR 60.0%
! CAGACCGGTTGGTTACGGAC+AGG + chr2_1:72347949-72347968 Msa0222930:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 72344815 72348256 72344815 ID=Msa0222930;Name=Msa0222930
chr2_1 mRNA 72344815 72348256 72344815 ID=Msa0222930-mRNA-1;Parent=Msa0222930;Name=Msa0222930-mRNA-1;_AED=0.07;_eAED=0.07;_QI=184|1|1|1|1|1|7|740|253
chr2_1 exon 72344815 72345037 72344815 ID=Msa0222930-mRNA-1:exon:20901;Parent=Msa0222930-mRNA-1
chr2_1 exon 72345526 72345601 72345526 ID=Msa0222930-mRNA-1:exon:20902;Parent=Msa0222930-mRNA-1
chr2_1 exon 72346162 72346285 72346162 ID=Msa0222930-mRNA-1:exon:20903;Parent=Msa0222930-mRNA-1
chr2_1 exon 72346512 72346596 72346512 ID=Msa0222930-mRNA-1:exon:20904;Parent=Msa0222930-mRNA-1
chr2_1 exon 72346732 72346864 72346732 ID=Msa0222930-mRNA-1:exon:20905;Parent=Msa0222930-mRNA-1
chr2_1 exon 72347038 72347132 72347038 ID=Msa0222930-mRNA-1:exon:20906;Parent=Msa0222930-mRNA-1
chr2_1 exon 72347307 72348256 72347307 ID=Msa0222930-mRNA-1:exon:20907;Parent=Msa0222930-mRNA-1
chr2_1 five_prime_UTR 72344815 72344998 72344815 ID=Msa0222930-mRNA-1:five_prime_utr;Parent=Msa0222930-mRNA-1
chr2_1 CDS 72344999 72345037 72344999 ID=Msa0222930-mRNA-1:cds;Parent=Msa0222930-mRNA-1
chr2_1 CDS 72345526 72345601 72345526 ID=Msa0222930-mRNA-1:cds;Parent=Msa0222930-mRNA-1
chr2_1 CDS 72346162 72346285 72346162 ID=Msa0222930-mRNA-1:cds;Parent=Msa0222930-mRNA-1
chr2_1 CDS 72346512 72346596 72346512 ID=Msa0222930-mRNA-1:cds;Parent=Msa0222930-mRNA-1
chr2_1 CDS 72346732 72346864 72346732 ID=Msa0222930-mRNA-1:cds;Parent=Msa0222930-mRNA-1
chr2_1 CDS 72347038 72347132 72347038 ID=Msa0222930-mRNA-1:cds;Parent=Msa0222930-mRNA-1
chr2_1 CDS 72347307 72347516 72347307 ID=Msa0222930-mRNA-1:cds;Parent=Msa0222930-mRNA-1
chr2_1 three_prime_UTR 72347517 72348256 72347517 ID=Msa0222930-mRNA-1:three_prime_utr;Parent=Msa0222930-mRNA-1
Gene Sequence

>Msa0222930

ATGGGCAGAAAATTCTTCGTCGGTGGTAACTGGAAATGCAATGGAACCACTGAAGAGGTGAAGAAAATCGTTGGTACTTTGAACGAGGCTCAAGTTCCCGGAGAAGATGTTGTTGAGGTTGTTGTGAGCCCCCCTTATGTGTTCCTTCCCTTTGTGAAAAGTCTACTTCGCTCTGACTTCCATGTTGCTGCTCAAAACGCTTGGGTTCGCAAAGGTGGTGCTTACACCGGGGAGATTAGTGCTGAAATGCTTGTTAATTTGGATATTCCTTGGGTTATTCTTGGCCACTCTGAGCGAAGGCAGCTTCTAAATGAAACAAATGAGTTTGTGGGAGACAAAGTTGCATATGCACTCTCACTAGGGAGGAAAGTCATTGCTTGCATTGGGGAGACTCTTGAACAACGTGAAGCTGGTACTACAATAGCTGTTGTTGCTGAGCAAACAAAGGCAATTGCAGACAAAGTATCAAACTGGGATAATGTTGTTTTGGCATATGAGCCAGTTTGGGCCATTGGGACTGGAAAGGTTGCTTCTCCTGCTCAGGCTCAAGAGGTCCATGCTGCTTTAAGGAAATGGGTTCACGACAACGTAGGTGCTGAAGTTGCTGCCTCTGTAAGGATTATCTATGGAGGTTCTGTAAACGGAGGAAACTCCAAAGAATTGGCATCTCAGCCTGATATTGATGGATTTTTGGTTGGCGGTGCTTCCTTGAAGCCAGAGTTCATTGACATCATCAACTCTGCCACAGTGAAGAAGAACTGA
Protein sequence

>Msa0222930

MGRKFFVGGNWKCNGTTEEVKKIVGTLNEAQVPGEDVVEVVVSPPYVFLPFVKSLLRSDFHVAAQNAWVRKGGAYTGEISAEMLVNLDIPWVILGHSERRQLLNETNEFVGDKVAYALSLGRKVIACIGETLEQREAGTTIAVVAEQTKAIADKVSNWDNVVLAYEPVWAIGTGKVASPAQAQEVHAALRKWVHDNVGAEVAASVRIIYGGSVNGGNSKELASQPDIDGFLVGGASLKPEFIDIINSATVKKN*