AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0264360

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0264360 MtrunA17_Chr2g0328161 100.000 253 0 0 1 253 1 253 0.0 513
Msa0264360 MtrunA17_Chr7g0264951 63.200 250 89 2 4 252 62 309 3.99e-110 320
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0264360 AT3G55440.1 81.423 253 47 0 1 253 1 253 4.40e-154 429
Msa0264360 AT2G21170.3 63.600 250 88 2 4 252 65 312 1.79e-109 318
Msa0264360 AT2G21170.1 63.600 250 88 2 4 252 65 312 1.79e-109 318
Msa0264360 AT2G21170.2 62.083 240 88 2 14 252 66 303 6.90e-100 293

Find 118 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAGGTCCATGCTGCTTTA+AGG 0.106869 2_2:+64268204 Msa0264360:intron
TGCTGAAATGCTTGTTAATT+TGG 0.126755 2_2:+64267414 Msa0264360:CDS
TACTACATGCTAGCTCTTTA+TGG 0.167583 2_2:-64268951 None:intergenic
CTTATACATTACGAGGTTTC+AGG 0.215165 2_2:+64269138 Msa0264360:three_prime_UTR
TTATTATAAACAAATATAAT+AGG 0.225661 2_2:+64269027 Msa0264360:three_prime_UTR
GATATTCCTTGGGTTATTCT+TGG 0.231384 2_2:+64267436 Msa0264360:CDS
TTGCATTATAAGAGCCTATT+AGG 0.248380 2_2:+64265740 Msa0264360:five_prime_UTR
GCTTAAAGCATATTCTTCTT+TGG 0.258020 2_2:+64265711 Msa0264360:five_prime_UTR
ATAAGTGTATTGAATCAATT+TGG 0.259889 2_2:+64268850 Msa0264360:three_prime_UTR
TGTGTTATGTCTTGAGGATT+TGG 0.279401 2_2:+64268710 Msa0264360:three_prime_UTR
AAACTGGGATAATGTTGTTT+TGG 0.298614 2_2:+64267950 Msa0264360:CDS
TCTCTGCTGAACTATGTTCT+AGG 0.305712 2_2:+64268810 Msa0264360:three_prime_UTR
ACGTTTATCGCCATTGTCTT+AGG 0.313974 2_2:+64268661 Msa0264360:three_prime_UTR
TCCATGCTGCTTTAAGGAAA+TGG 0.316327 2_2:+64268210 Msa0264360:CDS
CTCATCGGTGGGCGATGAAT+TGG 0.317824 2_2:+64268551 Msa0264360:three_prime_UTR
AGGCTGAGATGCCAATTCTT+TGG 0.323629 2_2:-64268309 None:intergenic
AATTCTTCGTCGGTGGTAAC+TGG 0.328227 2_2:+64265868 Msa0264360:CDS
TATTATAAACAAATATAATA+GGG 0.346860 2_2:+64269028 Msa0264360:three_prime_UTR
TGTTAATTTGGATATTCCTT+GGG 0.361669 2_2:+64267426 Msa0264360:CDS
GCATCTCAGCCTGATATTGA+TGG 0.369973 2_2:+64268320 Msa0264360:CDS
TTGCAGACAAAGTATCAAAC+TGG 0.370984 2_2:+64267934 Msa0264360:intron
TTGTTAATTTGGATATTCCT+TGG 0.377981 2_2:+64267425 Msa0264360:CDS
GGAAAGTCATTGCTTGCATT+GGG 0.383771 2_2:+64267674 Msa0264360:CDS
TTTGGCATATGAGCCAGTTT+GGG 0.383886 2_2:+64267968 Msa0264360:CDS
GCCTCTGTAAGGATTATCTA+TGG 0.388025 2_2:+64268263 Msa0264360:CDS
CCAGTTTGGGCCATTGGGAC+TGG 0.396885 2_2:+64267981 Msa0264360:CDS
AGTGACTGTCCTGTCCTAAT+AGG 0.402245 2_2:-64265754 None:intergenic
CGGAGGAAACTCCAAAGAAT+TGG 0.404230 2_2:+64268298 Msa0264360:CDS
CAAAGGGAAGGAACACATAA+GGG 0.405048 2_2:-64267080 None:intergenic
TATGAGCCAGTTTGGGCCAT+TGG 0.406432 2_2:+64267975 Msa0264360:CDS
TGATGACGACAATTCACTTC+CGG 0.406771 2_2:-64268777 None:intergenic
GTCAATGAACTCTGGCTTCA+AGG 0.409151 2_2:-64268363 None:intergenic
CGAGGTTTCAGGTTTAAACT+TGG 0.409231 2_2:+64269149 Msa0264360:three_prime_UTR
TCTAGAACAACAAGAAGAAA+AGG 0.412429 2_2:+64265795 Msa0264360:five_prime_UTR
GTCGTGACTGCCATAACATT+TGG 0.413861 2_2:+64268599 Msa0264360:three_prime_UTR
GTGCTTACACCGGGGAGATT+AGG 0.414986 2_2:+64267166 Msa0264360:CDS
ATCTATGGAGGTTCTGTAAA+CGG 0.416225 2_2:+64268278 Msa0264360:CDS
ATGAGCCAGTTTGGGCCATT+GGG 0.421695 2_2:+64267976 Msa0264360:CDS
TTGAACGAGGCTCAAGTTCC+CGG 0.427132 2_2:+64266471 Msa0264360:CDS
TTATTACTAACCAAATGTTA+TGG 0.428730 2_2:-64268609 None:intergenic
TTAAGTAAAACACGGGCGTT+CGG 0.431142 2_2:-64268634 None:intergenic
CCAGTCCCAATGGCCCAAAC+TGG 0.435261 2_2:-64267981 None:intergenic
CCATGCTGCTTTAAGGAAAT+GGG 0.440149 2_2:+64268211 Msa0264360:CDS
AAAGGTTGCTTCTCCTGCTC+AGG 0.446019 2_2:+64268004 Msa0264360:CDS
TGCTGCTTTCCAAGCTGCTT+CGG 0.446387 2_2:+64268441 Msa0264360:three_prime_UTR
GGCGACAGACCGGTTGGTTA+CGG 0.448337 2_2:+64268871 Msa0264360:three_prime_UTR
GAGCAAACAAAGGCAATTGC+AGG 0.449292 2_2:+64267745 Msa0264360:CDS
AGGAAAGTCATTGCTTGCAT+TGG 0.454029 2_2:+64267673 Msa0264360:CDS
TGTTGCTGCTCAAAACGCTT+GGG 0.460320 2_2:+64267131 Msa0264360:CDS
CAGAAATTTCCGAAGCAGCT+TGG 0.467398 2_2:-64268450 None:intergenic
CTGAAGTTGCTGCCTCTGTA+AGG 0.468253 2_2:+64268252 Msa0264360:CDS
TATCATCCGCTACAGTTTGT+GGG 0.468679 2_2:+64267619 Msa0264360:intron
ATCGCCCACCGATGAGGTAA+AGG 0.469952 2_2:-64268544 None:intergenic
CTCACCAACAACATCTTCTC+CGG 0.472241 2_2:-64266490 None:intergenic
GCAAAGGTGGTGCTTACACC+GGG 0.472783 2_2:+64267157 Msa0264360:CDS
AACAAATATAATAGGGGAGT+GGG 0.482103 2_2:+64269035 Msa0264360:three_prime_UTR
ATGTTGCTGCTCAAAACGCT+TGG 0.482349 2_2:+64267130 Msa0264360:CDS
CAGGCTGAACCCATTGATCA+AGG 0.495327 2_2:+64268895 Msa0264360:three_prime_UTR
GTTGCATATGCACTCTCACT+AGG 0.495343 2_2:+64267649 Msa0264360:CDS
CGCAAAGGTGGTGCTTACAC+CGG 0.498016 2_2:+64267156 Msa0264360:CDS
CTAGAACAACAAGAAGAAAA+GGG 0.510864 2_2:+64265796 Msa0264360:five_prime_UTR
TCAGCCTGTCCGTAACCAAC+CGG 0.512292 2_2:-64268880 None:intergenic
TTGGATTGTGTTATGTCTTG+AGG 0.512893 2_2:+64268704 Msa0264360:intron
TCGCCCACCGATGAGGTAAA+GGG 0.517509 2_2:-64268543 None:intergenic
AAACAAATATAATAGGGGAG+TGG 0.521187 2_2:+64269034 Msa0264360:three_prime_UTR
AACGCTTGGGTTCGCAAAGG+TGG 0.525911 2_2:+64267144 Msa0264360:CDS
ACTACCTCTTGAGCCTGAGC+AGG 0.528540 2_2:-64268017 None:intergenic
ACTCTTGAACAACGTGAAGC+TGG 0.531573 2_2:+64267700 Msa0264360:CDS
ACAAAGGGAAGGAACACATA+AGG 0.532183 2_2:-64267081 None:intergenic
TTCTCCTGCTCAGGCTCAAG+AGG 0.539203 2_2:+64268013 Msa0264360:CDS
GTTCCCGGAGAAGATGTTGT+TGG 0.540063 2_2:+64266486 Msa0264360:CDS
CTCTGCTGAACTATGTTCTA+GGG 0.543887 2_2:+64268811 Msa0264360:three_prime_UTR
TAAACACAAGTTCAAAGCAT+TGG 0.547440 2_2:-64268500 None:intergenic
GAGGTTTCAGGTTTAAACTT+GGG 0.552233 2_2:+64269150 Msa0264360:three_prime_UTR
ATTATAAACAAATATAATAG+GGG 0.554042 2_2:+64269029 Msa0264360:three_prime_UTR
TTGGGCCATTGGGACTGGAA+AGG 0.554351 2_2:+64267986 Msa0264360:CDS
CCCATTTCCTTAAAGCAGCA+TGG 0.555610 2_2:-64268211 None:intergenic
GAATCAATTTGGCGACAGAC+CGG 0.559054 2_2:+64268861 Msa0264360:three_prime_UTR
CAATTTGGCGACAGACCGGT+TGG 0.561018 2_2:+64268865 Msa0264360:three_prime_UTR
TCACCAACAACATCTTCTCC+GGG 0.561852 2_2:-64266489 None:intergenic
TTCTTGGCCACTCTGAGCGA+AGG 0.565910 2_2:+64267452 Msa0264360:CDS
TTATAAGAGCCTATTAGGAC+AGG 0.566780 2_2:+64265745 Msa0264360:five_prime_UTR
TTTCCCTTTACCTCATCGGT+GGG 0.569814 2_2:+64268540 Msa0264360:three_prime_UTR
TCGTAATGTATAAGTTGAAG+TGG 0.578365 2_2:-64269129 None:intergenic
TTGATGATGTCAATGAACTC+TGG 0.578436 2_2:-64268371 None:intergenic
GCTTCTAAATGAAACAAATG+AGG 0.602961 2_2:+64267477 Msa0264360:CDS
ACTTCAACTTATACATTACG+AGG 0.604440 2_2:+64269131 Msa0264360:three_prime_UTR
TATCATCAGACCTAAGACAA+TGG 0.607431 2_2:-64268671 None:intergenic
GAAGAGGTGAAGAAAATCGT+TGG 0.610298 2_2:+64266444 Msa0264360:CDS
AGCAACCTTTCCAGTCCCAA+TGG 0.611585 2_2:-64267991 None:intergenic
CAATTCATCGCCCACCGATG+AGG 0.616929 2_2:-64268550 None:intergenic
ATGGGCAGAAAATTCTTCGT+CGG 0.618039 2_2:+64265858 Msa0264360:CDS
TTGCATATGCACTCTCACTA+GGG 0.619513 2_2:+64267650 Msa0264360:CDS
GAATTGTCGTCATCACAGAA+AGG 0.621483 2_2:+64268785 Msa0264360:three_prime_UTR
AAAGGGAAGGAACACATAAG+GGG 0.623658 2_2:-64267079 None:intergenic
GGCAGAAAATTCTTCGTCGG+TGG 0.629014 2_2:+64265861 Msa0264360:CDS
GAAAGTCATTGCTTGCATTG+GGG 0.633688 2_2:+64267675 Msa0264360:CDS
AGGCTGAACCCATTGATCAA+GGG 0.634403 2_2:+64268896 Msa0264360:three_prime_UTR
AAGGGAAGGAACACATAAGG+GGG 0.637952 2_2:-64267078 None:intergenic
TGCAGACAAAGTATCAAACT+GGG 0.639857 2_2:+64267935 Msa0264360:intron
CAGACCGGTTGGTTACGGAC+AGG 0.641537 2_2:+64268876 Msa0264360:three_prime_UTR
CAAAACGCTTGGGTTCGCAA+AGG 0.648775 2_2:+64267141 Msa0264360:CDS
TGTTGTTGCTGAGCAAACAA+AGG 0.653424 2_2:+64267735 Msa0264360:CDS
TCTGTAAGGATTATCTATGG+AGG 0.664957 2_2:+64268266 Msa0264360:CDS
ACGTACAGTTAAGTAAAACA+CGG 0.667629 2_2:-64268642 None:intergenic
TCCATAGATAATCCTTACAG+AGG 0.672902 2_2:-64268264 None:intergenic
GCAGAATGGAACCACTGAAG+AGG 0.675685 2_2:+64266428 Msa0264360:intron
CATATGCACTCTCACTAGGG+AGG 0.678402 2_2:+64267653 Msa0264360:CDS
GAAGCTGCCTTCGCTCAGAG+TGG 0.679228 2_2:-64267459 None:intergenic
GAGTGGCCAAGAATAACCCA+AGG 0.679606 2_2:-64267442 None:intergenic
CGTACAGTTAAGTAAAACAC+GGG 0.684223 2_2:-64268641 None:intergenic
GATAACATACCTAATCTCCC+CGG 0.689404 2_2:-64267175 None:intergenic
TATGGAGGTTCTGTAAACGG+AGG 0.700353 2_2:+64268281 Msa0264360:CDS
AATCGTTGGTACTTTGAACG+AGG 0.707863 2_2:+64266458 Msa0264360:CDS
AAATGGGTTCACGACAACGT+AGG 0.725939 2_2:+64268227 Msa0264360:CDS
ACTTCAGTTCTTCTTCACTG+TGG 0.744514 2_2:-64268399 None:intergenic
AGGGAAGGAACACATAAGGG+GGG 0.750761 2_2:-64267077 None:intergenic
CAAAGGTGGTGCTTACACCG+GGG 0.762885 2_2:+64267158 Msa0264360:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATATTTAGTTAAAAATTTA+AGG - chr2_2:64266855-64266874 None:intergenic 10.0%
!!! TCAATAAAAATAATTTTTGT+TGG - chr2_2:64266982-64267001 None:intergenic 10.0%
!!! AAATTTTAAAAACAGTATAT+AGG - chr2_2:64268047-64268066 None:intergenic 10.0%
!! ATTATAAACAAATATAATAG+GGG + chr2_2:64269029-64269048 Msa0264360:three_prime_UTR 10.0%
!!! TGATTTTCTCAAAATATTAA+CGG - chr2_2:64266158-64266177 None:intergenic 15.0%
!! TGATGAAATAAATCAAATAA+TGG - chr2_2:64267867-64267886 None:intergenic 15.0%
!! GTTTCAGAATTAAGATTTAT+AGG - chr2_2:64266011-64266030 None:intergenic 20.0%
!! AACTATGTTAAAAATCAGAT+CGG + chr2_2:64266028-64266047 Msa0264360:intron 20.0%
!! GTATATAGACTATATATTTC+TGG + chr2_2:64266376-64266395 Msa0264360:intron 20.0%
!!! AGTAAATTTCAGTATTGATT+TGG + chr2_2:64266600-64266619 Msa0264360:intron 20.0%
!!! ATAAGAAATTTTTTTCCAAG+AGG + chr2_2:64266668-64266687 Msa0264360:intron 20.0%
!!! TAAGAAATTTTTTTCCAAGA+GGG + chr2_2:64266669-64266688 Msa0264360:intron 20.0%
!!! TCTCATTTTAATAATGCAAA+GGG - chr2_2:64266721-64266740 None:intergenic 20.0%
!! TTAACTAAATATCAAGTTTC+TGG + chr2_2:64266862-64266881 Msa0264360:intron 20.0%
!! TACAGAGATACAAAAAAAAA+AGG - chr2_2:64266932-64266951 None:intergenic 20.0%
!! TTATTACTAACCAAATGTTA+TGG - chr2_2:64268612-64268631 None:intergenic 20.0%
!! ATAAGTGTATTGAATCAATT+TGG + chr2_2:64268850-64268869 Msa0264360:three_prime_UTR 20.0%
! AATCATCGAAATCAAAAACA+TGG + chr2_2:64265839-64265858 Msa0264360:exon 25.0%
! ATCATCGAAATCAAAAACAT+GGG + chr2_2:64265840-64265859 Msa0264360:exon 25.0%
! CAACTAAAAACTAACTAGAT+CGG - chr2_2:64266113-64266132 None:intergenic 25.0%
!!! ATATTTCTGGTGATTCATAT+TGG + chr2_2:64266389-64266408 Msa0264360:intron 25.0%
!!! GTCTCATTTTAATAATGCAA+AGG - chr2_2:64266722-64266741 None:intergenic 25.0%
! GAGACTTAAATTCATTATAC+AGG + chr2_2:64266737-64266756 Msa0264360:intron 25.0%
!! ACGAAATTTGCTTATCTATT+TGG + chr2_2:64266828-64266847 Msa0264360:intron 25.0%
! AAATAAATCGAAGATCAGTA+CGG - chr2_2:64267200-64267219 None:intergenic 25.0%
!! TACTGATCTTCGATTTATTT+TGG + chr2_2:64267200-64267219 Msa0264360:intron 25.0%
! ACAAACACAAATTAAACGAT+AGG - chr2_2:64267374-64267393 None:intergenic 25.0%
!! TTGTTAATTTGGATATTCCT+TGG + chr2_2:64267425-64267444 Msa0264360:CDS 25.0%
!! TGTTAATTTGGATATTCCTT+GGG + chr2_2:64267426-64267445 Msa0264360:CDS 25.0%
!! TGTTGTCTTGATAATATTGA+TGG + chr2_2:64268174-64268193 Msa0264360:intron 25.0%
!! GGATTTTTGTTAAGTTTCTA+TGG + chr2_2:64268572-64268591 Msa0264360:three_prime_UTR 25.0%
!!! TTTCTTTTTTCTCATCTCAA+GGG - chr2_2:64268737-64268756 None:intergenic 25.0%
!!! ATTTCTTTTTTCTCATCTCA+AGG - chr2_2:64268738-64268757 None:intergenic 25.0%
! AAAAATAACCCTTGATCAAT+GGG - chr2_2:64268907-64268926 None:intergenic 25.0%
!! GAATTAACGATTTTTGTCAT+AGG - chr2_2:64269067-64269086 None:intergenic 25.0%
GCTTAAAGCATATTCTTCTT+TGG + chr2_2:64265711-64265730 Msa0264360:five_prime_UTR 30.0%
TTGCATTATAAGAGCCTATT+AGG + chr2_2:64265740-64265759 Msa0264360:five_prime_UTR 30.0%
! TCTAGAACAACAAGAAGAAA+AGG + chr2_2:64265795-64265814 Msa0264360:five_prime_UTR 30.0%
CTAGAACAACAAGAAGAAAA+GGG + chr2_2:64265796-64265815 Msa0264360:five_prime_UTR 30.0%
TTTCACTGTGAAATTCATGT+TGG + chr2_2:64266061-64266080 Msa0264360:intron 30.0%
AACTAACTAGATCGGAAAAA+GGG - chr2_2:64266105-64266124 None:intergenic 30.0%
AAACTAACTAGATCGGAAAA+AGG - chr2_2:64266106-64266125 None:intergenic 30.0%
CATAGATCCTTATTCCATAT+AGG + chr2_2:64266236-64266255 Msa0264360:intron 30.0%
AGCATATCCTATATGGAATA+AGG - chr2_2:64266246-64266265 None:intergenic 30.0%
TTGTTTGAGCATATCCTATA+TGG - chr2_2:64266253-64266272 None:intergenic 30.0%
AAAAGAAAAACAGTTCCACA+AGG - chr2_2:64266327-64266346 None:intergenic 30.0%
!! GTGGAACTGTTTTTCTTTTT+AGG + chr2_2:64266328-64266347 Msa0264360:intron 30.0%
TTTACTGCTACGTAAATATG+CGG - chr2_2:64266697-64266716 None:intergenic 30.0%
AGGATATTGCAATATCAGTA+AGG - chr2_2:64266912-64266931 None:intergenic 30.0%
TCAGTGGTTTATTAATAGTG+TGG + chr2_2:64267327-64267346 Msa0264360:intron 30.0%
! TGCTGAAATGCTTGTTAATT+TGG + chr2_2:64267414-64267433 Msa0264360:CDS 30.0%
GCTTCTAAATGAAACAAATG+AGG + chr2_2:64267477-64267496 Msa0264360:CDS 30.0%
!!! ACAAACTTTTTTGTTGTCTC+TGG + chr2_2:64267501-64267520 Msa0264360:intron 30.0%
!!! CAAACTTTTTTGTTGTCTCT+GGG + chr2_2:64267502-64267521 Msa0264360:intron 30.0%
GGATGATAAAGCAGTAAAAT+GGG - chr2_2:64267607-64267626 None:intergenic 30.0%
TTTGTCTGCAAGTAAAATTG+AGG - chr2_2:64267925-64267944 None:intergenic 30.0%
!! AAACTGGGATAATGTTGTTT+TGG + chr2_2:64267950-64267969 Msa0264360:CDS 30.0%
AAGAATGAGATGTATCAACA+GGG - chr2_2:64268104-64268123 None:intergenic 30.0%
AAAGAATGAGATGTATCAAC+AGG - chr2_2:64268105-64268124 None:intergenic 30.0%
!!! ATATTGATGGTTTTTTGCCT+AGG + chr2_2:64268187-64268206 Msa0264360:intron 30.0%
ACCAAAAATCCATCAATATC+AGG - chr2_2:64268332-64268351 None:intergenic 30.0%
!!! GATATTGATGGATTTTTGGT+TGG + chr2_2:64268332-64268351 Msa0264360:CDS 30.0%
TAAACACAAGTTCAAAGCAT+TGG - chr2_2:64268503-64268522 None:intergenic 30.0%
ACGTACAGTTAAGTAAAACA+CGG - chr2_2:64268645-64268664 None:intergenic 30.0%
!!! GCAGTTGTTTTTACTAAAAC+CGG + chr2_2:64268758-64268777 Msa0264360:three_prime_UTR 30.0%
CAAAAATAACCCTTGATCAA+TGG - chr2_2:64268908-64268927 None:intergenic 30.0%
!!! TCAAGGGTTATTTTTGTTCA+TGG + chr2_2:64268912-64268931 Msa0264360:three_prime_UTR 30.0%
AAACAAATATAATAGGGGAG+TGG + chr2_2:64269034-64269053 Msa0264360:three_prime_UTR 30.0%
AACAAATATAATAGGGGAGT+GGG + chr2_2:64269035-64269054 Msa0264360:three_prime_UTR 30.0%
TCTCTTAACAAAAATCGAAG+TGG + chr2_2:64269100-64269119 Msa0264360:three_prime_UTR 30.0%
! TCGTAATGTATAAGTTGAAG+TGG - chr2_2:64269132-64269151 None:intergenic 30.0%
ACTTCAACTTATACATTACG+AGG + chr2_2:64269131-64269150 Msa0264360:three_prime_UTR 30.0%
TTATAAGAGCCTATTAGGAC+AGG + chr2_2:64265745-64265764 Msa0264360:five_prime_UTR 35.0%
GGCAATTATGAATGCTAACA+TGG - chr2_2:64266306-64266325 None:intergenic 35.0%
TAGCATTCATAATTGCCTTG+TGG + chr2_2:64266309-64266328 Msa0264360:intron 35.0%
TTCTGGTGATTCATATTGGT+TGG + chr2_2:64266393-64266412 Msa0264360:intron 35.0%
! TCTGGTGATTCATATTGGTT+GGG + chr2_2:64266394-64266413 Msa0264360:intron 35.0%
ATTCATTATACAGGCATGCA+TGG + chr2_2:64266746-64266765 Msa0264360:intron 35.0%
TTGTCAGCACATGCAAATAT+TGG - chr2_2:64267024-64267043 None:intergenic 35.0%
!! TGTGCTGACAAATGTTTTTC+TGG + chr2_2:64267033-64267052 Msa0264360:intron 35.0%
!!! ATGTTTTTCTGGTTCTACAG+AGG + chr2_2:64267044-64267063 Msa0264360:intron 35.0%
! CGAAGTAGACTTTTCACAAA+GGG - chr2_2:64267099-64267118 None:intergenic 35.0%
GTTCTGACACATGATTCAAT+CGG - chr2_2:64267281-64267300 None:intergenic 35.0%
AATCATGTGTCAGAACTGTT+TGG + chr2_2:64267285-64267304 Msa0264360:intron 35.0%
! TCTTTCTCGTTTTGTATCAG+TGG + chr2_2:64267311-64267330 Msa0264360:intron 35.0%
GATATTCCTTGGGTTATTCT+TGG + chr2_2:64267436-64267455 Msa0264360:CDS 35.0%
CGGATGATAAAGCAGTAAAA+TGG - chr2_2:64267608-64267627 None:intergenic 35.0%
! TTGCAGACAAAGTATCAAAC+TGG + chr2_2:64267934-64267953 Msa0264360:intron 35.0%
! TGCAGACAAAGTATCAAACT+GGG + chr2_2:64267935-64267954 Msa0264360:intron 35.0%
TCCATAGATAATCCTTACAG+AGG - chr2_2:64268267-64268286 None:intergenic 35.0%
TCTGTAAGGATTATCTATGG+AGG + chr2_2:64268266-64268285 Msa0264360:CDS 35.0%
ATCTATGGAGGTTCTGTAAA+CGG + chr2_2:64268278-64268297 Msa0264360:CDS 35.0%
!!! GCCTGATATTGATGGATTTT+TGG + chr2_2:64268328-64268347 Msa0264360:CDS 35.0%
!!! ATTGATGGATTTTTGGTTGG+CGG + chr2_2:64268335-64268354 Msa0264360:CDS 35.0%
!! TTGATGATGTCAATGAACTC+TGG - chr2_2:64268374-64268393 None:intergenic 35.0%
! ATGTTTTCCCTTTACCTCAT+CGG + chr2_2:64268536-64268555 Msa0264360:three_prime_UTR 35.0%
CGTACAGTTAAGTAAAACAC+GGG - chr2_2:64268644-64268663 None:intergenic 35.0%
TATCATCAGACCTAAGACAA+TGG - chr2_2:64268674-64268693 None:intergenic 35.0%
! CTGATGATACTTGTGTTGTT+TGG + chr2_2:64268685-64268704 Msa0264360:intron 35.0%
TTGGATTGTGTTATGTCTTG+AGG + chr2_2:64268704-64268723 Msa0264360:intron 35.0%
TGTGTTATGTCTTGAGGATT+TGG + chr2_2:64268710-64268729 Msa0264360:three_prime_UTR 35.0%
! TACTACATGCTAGCTCTTTA+TGG - chr2_2:64268954-64268973 None:intergenic 35.0%
CTTATACATTACGAGGTTTC+AGG + chr2_2:64269138-64269157 Msa0264360:three_prime_UTR 35.0%
!! GAGGTTTCAGGTTTAAACTT+GGG + chr2_2:64269150-64269169 Msa0264360:three_prime_UTR 35.0%
ATGGGCAGAAAATTCTTCGT+CGG + chr2_2:64265858-64265877 Msa0264360:CDS 40.0%
AGAGTGAAGAGAGTGAAAAG+AGG - chr2_2:64265912-64265931 None:intergenic 40.0%
CATATGTGTTAGTGTCGTCT+TGG + chr2_2:64266194-64266213 Msa0264360:intron 40.0%
! TCGTCTTGGATCAAAAGTGT+TGG + chr2_2:64266208-64266227 Msa0264360:intron 40.0%
!! GGGTTGATTTTGTTGCAGAA+TGG + chr2_2:64266414-64266433 Msa0264360:intron 40.0%
! GATTTTCTTCACCTCTTCAG+TGG - chr2_2:64266442-64266461 None:intergenic 40.0%
GAAGAGGTGAAGAAAATCGT+TGG + chr2_2:64266444-64266463 Msa0264360:CDS 40.0%
AATCGTTGGTACTTTGAACG+AGG + chr2_2:64266458-64266477 Msa0264360:CDS 40.0%
! ATGTTGTTGGTGAGTCTTAG+TGG + chr2_2:64266499-64266518 Msa0264360:intron 40.0%
AAAGGGAAGGAACACATAAG+GGG - chr2_2:64267082-64267101 None:intergenic 40.0%
CAAAGGGAAGGAACACATAA+GGG - chr2_2:64267083-64267102 None:intergenic 40.0%
ACAAAGGGAAGGAACACATA+AGG - chr2_2:64267084-64267103 None:intergenic 40.0%
! GTAGACTTTTCACAAAGGGA+AGG - chr2_2:64267095-64267114 None:intergenic 40.0%
! GCGAAGTAGACTTTTCACAA+AGG - chr2_2:64267100-64267119 None:intergenic 40.0%
GATAACATACCTAATCTCCC+CGG - chr2_2:64267178-64267197 None:intergenic 40.0%
! TTTTTATCATCACTGCCGCA+AGG + chr2_2:64267237-64267256 Msa0264360:intron 40.0%
! TTTTATCATCACTGCCGCAA+GGG + chr2_2:64267238-64267257 Msa0264360:intron 40.0%
TCATACACAGCTAGCACAAA+GGG - chr2_2:64267544-64267563 None:intergenic 40.0%
TTATCATCCGCTACAGTTTG+TGG + chr2_2:64267618-64267637 Msa0264360:intron 40.0%
TATCATCCGCTACAGTTTGT+GGG + chr2_2:64267619-64267638 Msa0264360:intron 40.0%
TTGCATATGCACTCTCACTA+GGG + chr2_2:64267650-64267669 Msa0264360:CDS 40.0%
AGGAAAGTCATTGCTTGCAT+TGG + chr2_2:64267673-64267692 Msa0264360:CDS 40.0%
GGAAAGTCATTGCTTGCATT+GGG + chr2_2:64267674-64267693 Msa0264360:CDS 40.0%
GAAAGTCATTGCTTGCATTG+GGG + chr2_2:64267675-64267694 Msa0264360:CDS 40.0%
TGTTGTTGCTGAGCAAACAA+AGG + chr2_2:64267735-64267754 Msa0264360:CDS 40.0%
!!! TTCATCACTTGCTGTTTTGC+TGG + chr2_2:64267880-64267899 Msa0264360:intron 40.0%
! TTTTGGCATATGAGCCAGTT+TGG + chr2_2:64267967-64267986 Msa0264360:CDS 40.0%
TTTGGCATATGAGCCAGTTT+GGG + chr2_2:64267968-64267987 Msa0264360:CDS 40.0%
!! TCCATGCTGCTTTAAGGAAA+TGG + chr2_2:64268210-64268229 Msa0264360:CDS 40.0%
!! CCATGCTGCTTTAAGGAAAT+GGG + chr2_2:64268211-64268230 Msa0264360:CDS 40.0%
GCCTCTGTAAGGATTATCTA+TGG + chr2_2:64268263-64268282 Msa0264360:CDS 40.0%
ACTTCAGTTCTTCTTCACTG+TGG - chr2_2:64268402-64268421 None:intergenic 40.0%
TTAAGTAAAACACGGGCGTT+CGG - chr2_2:64268637-64268656 None:intergenic 40.0%
ACGTTTATCGCCATTGTCTT+AGG + chr2_2:64268661-64268680 Msa0264360:three_prime_UTR 40.0%
! TGATGACGACAATTCACTTC+CGG - chr2_2:64268780-64268799 None:intergenic 40.0%
GAATTGTCGTCATCACAGAA+AGG + chr2_2:64268785-64268804 Msa0264360:three_prime_UTR 40.0%
TCTCTGCTGAACTATGTTCT+AGG + chr2_2:64268810-64268829 Msa0264360:three_prime_UTR 40.0%
CTCTGCTGAACTATGTTCTA+GGG + chr2_2:64268811-64268830 Msa0264360:three_prime_UTR 40.0%
!! CGAGGTTTCAGGTTTAAACT+TGG + chr2_2:64269149-64269168 Msa0264360:three_prime_UTR 40.0%
AGTGACTGTCCTGTCCTAAT+AGG - chr2_2:64265757-64265776 None:intergenic 45.0%
AATTCTTCGTCGGTGGTAAC+TGG + chr2_2:64265868-64265887 Msa0264360:CDS 45.0%
TCACCAACAACATCTTCTCC+GGG - chr2_2:64266492-64266511 None:intergenic 45.0%
CTCACCAACAACATCTTCTC+CGG - chr2_2:64266493-64266512 None:intergenic 45.0%
GTAAATATGCGGTTCCCTCT+TGG - chr2_2:64266686-64266705 None:intergenic 45.0%
AAGGGAAGGAACACATAAGG+GGG - chr2_2:64267081-64267100 None:intergenic 45.0%
!! AAGCGTTTTGAGCAGCAACA+TGG - chr2_2:64267131-64267150 None:intergenic 45.0%
ATGTTGCTGCTCAAAACGCT+TGG + chr2_2:64267130-64267149 Msa0264360:CDS 45.0%
TGTTGCTGCTCAAAACGCTT+GGG + chr2_2:64267131-64267150 Msa0264360:CDS 45.0%
CATACACAGCTAGCACAAAG+GGG - chr2_2:64267543-64267562 None:intergenic 45.0%
GTCATACACAGCTAGCACAA+AGG - chr2_2:64267545-64267564 None:intergenic 45.0%
TTGTCTCCCACAAACTGTAG+CGG - chr2_2:64267628-64267647 None:intergenic 45.0%
GTTGCATATGCACTCTCACT+AGG + chr2_2:64267649-64267668 Msa0264360:CDS 45.0%
ACTCTTGAACAACGTGAAGC+TGG + chr2_2:64267700-64267719 Msa0264360:CDS 45.0%
GAGCAAACAAAGGCAATTGC+AGG + chr2_2:64267745-64267764 Msa0264360:CDS 45.0%
CCCATTTCCTTAAAGCAGCA+TGG - chr2_2:64268214-64268233 None:intergenic 45.0%
! AAATGGGTTCACGACAACGT+AGG + chr2_2:64268227-64268246 Msa0264360:CDS 45.0%
TATGGAGGTTCTGTAAACGG+AGG + chr2_2:64268281-64268300 Msa0264360:CDS 45.0%
CGGAGGAAACTCCAAAGAAT+TGG + chr2_2:64268298-64268317 Msa0264360:CDS 45.0%
AGGCTGAGATGCCAATTCTT+TGG - chr2_2:64268312-64268331 None:intergenic 45.0%
GCATCTCAGCCTGATATTGA+TGG + chr2_2:64268320-64268339 Msa0264360:CDS 45.0%
! GTCAATGAACTCTGGCTTCA+AGG - chr2_2:64268366-64268385 None:intergenic 45.0%
CAGAAATTTCCGAAGCAGCT+TGG - chr2_2:64268453-64268472 None:intergenic 45.0%
! TTTTCCCTTTACCTCATCGG+TGG + chr2_2:64268539-64268558 Msa0264360:three_prime_UTR 45.0%
TTTCCCTTTACCTCATCGGT+GGG + chr2_2:64268540-64268559 Msa0264360:three_prime_UTR 45.0%
GTCGTGACTGCCATAACATT+TGG + chr2_2:64268599-64268618 Msa0264360:three_prime_UTR 45.0%
GAATCAATTTGGCGACAGAC+CGG + chr2_2:64268861-64268880 Msa0264360:three_prime_UTR 45.0%
AGGCTGAACCCATTGATCAA+GGG + chr2_2:64268896-64268915 Msa0264360:three_prime_UTR 45.0%
!! ATATTTAGTTAAAAATTTAA+GGG - chr2_2:64266854-64266873 None:intergenic 5.0%
!! TTATTATAAACAAATATAAT+AGG + chr2_2:64269027-64269046 Msa0264360:three_prime_UTR 5.0%
!! TATTATAAACAAATATAATA+GGG + chr2_2:64269028-64269047 Msa0264360:three_prime_UTR 5.0%
GGCAGAAAATTCTTCGTCGG+TGG + chr2_2:64265861-64265880 Msa0264360:CDS 50.0%
GCAGAATGGAACCACTGAAG+AGG + chr2_2:64266428-64266447 Msa0264360:intron 50.0%
TTGAACGAGGCTCAAGTTCC+CGG + chr2_2:64266471-64266490 Msa0264360:CDS 50.0%
GTTCCCGGAGAAGATGTTGT+TGG + chr2_2:64266486-64266505 Msa0264360:CDS 50.0%
GGCAACTGCAGTGAATTCAG+TGG - chr2_2:64266891-64266910 None:intergenic 50.0%
AGGGAAGGAACACATAAGGG+GGG - chr2_2:64267080-64267099 None:intergenic 50.0%
CAAAACGCTTGGGTTCGCAA+AGG + chr2_2:64267141-64267160 Msa0264360:CDS 50.0%
CGAAAAGACCGAAACCCTTG+CGG - chr2_2:64267255-64267274 None:intergenic 50.0%
GAGTGGCCAAGAATAACCCA+AGG - chr2_2:64267445-64267464 None:intergenic 50.0%
CATATGCACTCTCACTAGGG+AGG + chr2_2:64267653-64267672 Msa0264360:CDS 50.0%
TATGAGCCAGTTTGGGCCAT+TGG + chr2_2:64267975-64267994 Msa0264360:CDS 50.0%
ATGAGCCAGTTTGGGCCATT+GGG + chr2_2:64267976-64267995 Msa0264360:CDS 50.0%
AGCAACCTTTCCAGTCCCAA+TGG - chr2_2:64267994-64268013 None:intergenic 50.0%
AAAGGTTGCTTCTCCTGCTC+AGG + chr2_2:64268004-64268023 Msa0264360:CDS 50.0%
CCTTAAAGCAGCATGGACCT+AGG - chr2_2:64268207-64268226 None:intergenic 50.0%
!! CCTAGGTCCATGCTGCTTTA+AGG + chr2_2:64268204-64268223 Msa0264360:intron 50.0%
CTGAAGTTGCTGCCTCTGTA+AGG + chr2_2:64268252-64268271 Msa0264360:CDS 50.0%
!! TGCTGCTTTCCAAGCTGCTT+CGG + chr2_2:64268441-64268460 Msa0264360:three_prime_UTR 50.0%
CAGGCTGAACCCATTGATCA+AGG + chr2_2:64268895-64268914 Msa0264360:three_prime_UTR 50.0%
AACGCTTGGGTTCGCAAAGG+TGG + chr2_2:64267144-64267163 Msa0264360:CDS 55.0%
CGCAAAGGTGGTGCTTACAC+CGG + chr2_2:64267156-64267175 Msa0264360:CDS 55.0%
GCAAAGGTGGTGCTTACACC+GGG + chr2_2:64267157-64267176 Msa0264360:CDS 55.0%
CAAAGGTGGTGCTTACACCG+GGG + chr2_2:64267158-64267177 Msa0264360:CDS 55.0%
GTGCTTACACCGGGGAGATT+AGG + chr2_2:64267166-64267185 Msa0264360:CDS 55.0%
CATCACTGCCGCAAGGGTTT+CGG + chr2_2:64267244-64267263 Msa0264360:intron 55.0%
TTCTTGGCCACTCTGAGCGA+AGG + chr2_2:64267452-64267471 Msa0264360:CDS 55.0%
TTGGGCCATTGGGACTGGAA+AGG + chr2_2:64267986-64268005 Msa0264360:CDS 55.0%
TTCTCCTGCTCAGGCTCAAG+AGG + chr2_2:64268013-64268032 Msa0264360:CDS 55.0%
ACTACCTCTTGAGCCTGAGC+AGG - chr2_2:64268020-64268039 None:intergenic 55.0%
TCGCCCACCGATGAGGTAAA+GGG - chr2_2:64268546-64268565 None:intergenic 55.0%
ATCGCCCACCGATGAGGTAA+AGG - chr2_2:64268547-64268566 None:intergenic 55.0%
CAATTCATCGCCCACCGATG+AGG - chr2_2:64268553-64268572 None:intergenic 55.0%
CTCATCGGTGGGCGATGAAT+TGG + chr2_2:64268551-64268570 Msa0264360:three_prime_UTR 55.0%
CAATTTGGCGACAGACCGGT+TGG + chr2_2:64268865-64268884 Msa0264360:three_prime_UTR 55.0%
TCAGCCTGTCCGTAACCAAC+CGG - chr2_2:64268883-64268902 None:intergenic 55.0%
GAAGCTGCCTTCGCTCAGAG+TGG - chr2_2:64267462-64267481 None:intergenic 60.0%
CCAGTCCCAATGGCCCAAAC+TGG - chr2_2:64267984-64268003 None:intergenic 60.0%
CCAGTTTGGGCCATTGGGAC+TGG + chr2_2:64267981-64268000 Msa0264360:CDS 60.0%
! GGCGACAGACCGGTTGGTTA+CGG + chr2_2:64268871-64268890 Msa0264360:three_prime_UTR 60.0%
! CAGACCGGTTGGTTACGGAC+AGG + chr2_2:64268876-64268895 Msa0264360:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 64265671 64269183 64265671 ID=Msa0264360;Name=Msa0264360
chr2_2 mRNA 64265671 64269183 64265671 ID=Msa0264360-mRNA-1;Parent=Msa0264360;Name=Msa0264360-mRNA-1;_AED=0.08;_eAED=0.08;_QI=187|1|1|1|0.85|0.75|8|740|253
chr2_2 exon 64265671 64265896 64265671 ID=Msa0264360-mRNA-1:exon:20000;Parent=Msa0264360-mRNA-1
chr2_2 exon 64266432 64266507 64266432 ID=Msa0264360-mRNA-1:exon:20001;Parent=Msa0264360-mRNA-1
chr2_2 exon 64267064 64267187 64267064 ID=Msa0264360-mRNA-1:exon:20002;Parent=Msa0264360-mRNA-1
chr2_2 exon 64267414 64267498 64267414 ID=Msa0264360-mRNA-1:exon:20003;Parent=Msa0264360-mRNA-1
chr2_2 exon 64267634 64267766 64267634 ID=Msa0264360-mRNA-1:exon:20004;Parent=Msa0264360-mRNA-1
chr2_2 exon 64267940 64268034 64267940 ID=Msa0264360-mRNA-1:exon:20005;Parent=Msa0264360-mRNA-1
chr2_2 exon 64268209 64268682 64268209 ID=Msa0264360-mRNA-1:exon:20006;Parent=Msa0264360-mRNA-1
chr2_2 exon 64268708 64269183 64268708 ID=Msa0264360-mRNA-1:exon:20007;Parent=Msa0264360-mRNA-1
chr2_2 five_prime_UTR 64265671 64265857 64265671 ID=Msa0264360-mRNA-1:five_prime_utr;Parent=Msa0264360-mRNA-1
chr2_2 CDS 64265858 64265896 64265858 ID=Msa0264360-mRNA-1:cds;Parent=Msa0264360-mRNA-1
chr2_2 CDS 64266432 64266507 64266432 ID=Msa0264360-mRNA-1:cds;Parent=Msa0264360-mRNA-1
chr2_2 CDS 64267064 64267187 64267064 ID=Msa0264360-mRNA-1:cds;Parent=Msa0264360-mRNA-1
chr2_2 CDS 64267414 64267498 64267414 ID=Msa0264360-mRNA-1:cds;Parent=Msa0264360-mRNA-1
chr2_2 CDS 64267634 64267766 64267634 ID=Msa0264360-mRNA-1:cds;Parent=Msa0264360-mRNA-1
chr2_2 CDS 64267940 64268034 64267940 ID=Msa0264360-mRNA-1:cds;Parent=Msa0264360-mRNA-1
chr2_2 CDS 64268209 64268418 64268209 ID=Msa0264360-mRNA-1:cds;Parent=Msa0264360-mRNA-1
chr2_2 three_prime_UTR 64268419 64268682 64268419 ID=Msa0264360-mRNA-1:three_prime_utr;Parent=Msa0264360-mRNA-1
chr2_2 three_prime_UTR 64268708 64269183 64268708 ID=Msa0264360-mRNA-1:three_prime_utr;Parent=Msa0264360-mRNA-1
Gene Sequence

>Msa0264360

ATGGGCAGAAAATTCTTCGTCGGTGGTAACTGGAAATGCAATGGAACCACTGAAGAGGTGAAGAAAATCGTTGGTACTTTGAACGAGGCTCAAGTTCCCGGAGAAGATGTTGTTGAGGTTGTTGTGAGCCCCCCTTATGTGTTCCTTCCCTTTGTGAAAAGTCTACTTCGCTCTGACTTCCATGTTGCTGCTCAAAACGCTTGGGTTCGCAAAGGTGGTGCTTACACCGGGGAGATTAGTGCTGAAATGCTTGTTAATTTGGATATTCCTTGGGTTATTCTTGGCCACTCTGAGCGAAGGCAGCTTCTAAATGAAACAAATGAGTTTGTGGGAGACAAAGTTGCATATGCACTCTCACTAGGGAGGAAAGTCATTGCTTGCATTGGGGAGACTCTTGAACAACGTGAAGCTGGTACTACAATAGCTGTTGTTGCTGAGCAAACAAAGGCAATTGCAGACAAAGTATCAAACTGGGATAATGTTGTTTTGGCATATGAGCCAGTTTGGGCCATTGGGACTGGAAAGGTTGCTTCTCCTGCTCAGGCTCAAGAGGTCCATGCTGCTTTAAGGAAATGGGTTCACGACAACGTAGGTGCTGAAGTTGCTGCCTCTGTAAGGATTATCTATGGAGGTTCTGTAAACGGAGGAAACTCCAAAGAATTGGCATCTCAGCCTGATATTGATGGATTTTTGGTTGGCGGTGCTTCCTTGAAGCCAGAGTTCATTGACATCATCAACTCTGCCACAGTGAAGAAGAACTGA
Protein sequence

>Msa0264360

MGRKFFVGGNWKCNGTTEEVKKIVGTLNEAQVPGEDVVEVVVSPPYVFLPFVKSLLRSDFHVAAQNAWVRKGGAYTGEISAEMLVNLDIPWVILGHSERRQLLNETNEFVGDKVAYALSLGRKVIACIGETLEQREAGTTIAVVAEQTKAIADKVSNWDNVVLAYEPVWAIGTGKVASPAQAQEVHAALRKWVHDNVGAEVAASVRIIYGGSVNGGNSKELASQPDIDGFLVGGASLKPEFIDIINSATVKKN*