AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0328400

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0328400 MtrunA17_Chr2g0309681 98.214 392 4 3 1 391 1 390 0.0 786
Msa0328400 MtrunA17_Chr8g0356301 77.215 395 73 8 1 390 1 383 0.0 568
Msa0328400 MtrunA17_Chr4g0073991 51.813 193 91 1 199 389 145 337 1.79e-69 223
Msa0328400 MtrunA17_Chr5g0420791 48.958 192 96 1 200 389 110 301 1.21e-68 219
Msa0328400 MtrunA17_Chr8g0366071 48.500 200 98 2 195 389 109 308 7.06e-68 217
Msa0328400 MtrunA17_Chr4g0001371 49.738 191 94 1 199 387 134 324 3.02e-67 217
Msa0328400 MtrunA17_Chr3g0141411 45.495 222 116 3 169 387 97 316 7.56e-65 210
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0328400 AT3G12710.1 70.609 279 63 4 115 389 46 309 3.04e-139 399
Msa0328400 AT5G44680.1 51.392 395 146 8 1 391 1 353 3.33e-135 391
Msa0328400 AT1G75090.1 53.723 188 85 1 202 387 119 306 4.80e-74 234
Msa0328400 AT5G57970.1 50.000 212 99 2 183 387 130 341 1.31e-72 231
Msa0328400 AT5G57970.2 50.000 212 99 2 183 387 130 341 1.31e-72 231
Msa0328400 AT1G80850.1 54.839 186 82 1 202 385 134 319 3.42e-71 226
Msa0328400 AT1G13635.2 50.510 196 95 1 196 389 109 304 3.56e-71 226
Msa0328400 AT1G13635.1 50.510 196 95 1 196 389 109 304 3.56e-71 226
Msa0328400 AT1G15970.1 50.769 195 84 2 202 389 145 334 8.92e-67 216
Msa0328400 AT1G15970.2 50.769 195 84 2 202 389 145 334 8.92e-67 216

Find 112 sgRNAs with CRISPR-Local

Find 181 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCACTTGCAATGCACATTAT+TGG 0.261232 2_4:-40830035 Msa0328400:CDS
CATTTGCTTATCAGTCAAAT+TGG 0.271540 2_4:+40831342 None:intergenic
TTCATTTGACAAATACATTT+GGG 0.277104 2_4:-40830248 Msa0328400:CDS
TATCCCACACAGGCTGTTAA+AGG 0.287777 2_4:+40829288 None:intergenic
AGATCAAGATCATTTATTAA+AGG 0.297955 2_4:-40829611 Msa0328400:three_prime_UTR
TAAGAAGTGGTGGGAGTGTT+AGG 0.297982 2_4:-40829418 Msa0328400:three_prime_UTR
GGTAATGGAGAAAGTGATTT+TGG 0.299287 2_4:+40832771 None:intergenic
ATCTCAAATCAATACAAATT+TGG 0.303182 2_4:-40830205 Msa0328400:CDS
TTTCCTCTCTTTATAGCTAA+TGG 0.311064 2_4:+40832669 None:intergenic
CTAGTAGGTGACTTTGGTTT+TGG 0.311738 2_4:+40832699 None:intergenic
AAAATGAAGATTGCTCATTA+TGG 0.312266 2_4:-40832429 Msa0328400:CDS
GAGTTTCTTCTTTCAAGATT+TGG 0.322751 2_4:+40832810 None:intergenic
CATAATGAAAACTCCAACTT+TGG 0.328226 2_4:-40832595 Msa0328400:CDS
GGGTCAATTAATCTCTTATA+TGG 0.340630 2_4:+40830009 None:intergenic
GTTCATTTGACAAATACATT+TGG 0.343231 2_4:-40830249 Msa0328400:CDS
TGATTAAGTGGTCATTTGTT+AGG 0.343650 2_4:+40830068 None:intergenic
GAAGCATAGCTGCTGTTAGA+AGG 0.354512 2_4:-40832482 Msa0328400:CDS
TACAGTTACTTCTTTAGCCT+TGG 0.358427 2_4:+40832911 None:intergenic
ATGTCTATTAGTTCAGAATA+TGG 0.363323 2_4:-40831319 Msa0328400:CDS
GAAGTAGTAGTAGTCTTGTT+TGG 0.369631 2_4:+40832744 None:intergenic
TGAAGAAACGCCTAGACTTC+AGG 0.370308 2_4:-40831497 Msa0328400:intron
AAGAGAGGAAATGACAATAA+TGG 0.373376 2_4:-40832657 Msa0328400:CDS
CAGCATGTGCATGTGCAAAT+AGG 0.375156 2_4:-40829388 Msa0328400:three_prime_UTR
ACACTCCCACCACTTCTTAA+CGG 0.386475 2_4:+40829422 None:intergenic
TGTTGTGTATAGTATTAGAT+AGG 0.387658 2_4:-40829887 Msa0328400:three_prime_UTR
AGGGAAATGTTGTGAGGGAT+TGG 0.388202 2_4:+40833039 None:intergenic
AGGAGTAATAGTGTTAAGAT+TGG 0.390149 2_4:+40832994 None:intergenic
CTAACAGCAGCTATGCTTCC+CGG 0.396009 2_4:+40832486 None:intergenic
TGCTTATCATGATGAAGAAT+GGG 0.396248 2_4:-40831983 Msa0328400:CDS
GTCTTGTTTGGAAGTGGTAA+TGG 0.397911 2_4:+40832756 None:intergenic
AAAGAAAATAGAGTTTGTTA+AGG 0.398022 2_4:+40829240 None:intergenic
AAATAATGCAAAGTCCTTGT+AGG 0.402213 2_4:+40829316 None:intergenic
TTACAGGTTAAGAAAGGCTT+TGG 0.404283 2_4:-40830271 Msa0328400:intron
TTCTTTGATCACTGATTCAC+CGG 0.407327 2_4:-40832505 Msa0328400:CDS
AGAGCACTTGAAGGATCAAT+AGG 0.410745 2_4:+40832372 None:intergenic
AATTTGTATTGATTTGAGAT+TGG 0.419291 2_4:+40830208 None:intergenic
TATGCTGACTATAGTTTACT+TGG 0.422375 2_4:-40829917 Msa0328400:three_prime_UTR
GTTATTAAGAGAAATAGATT+TGG 0.426782 2_4:-40829741 Msa0328400:three_prime_UTR
CTAGTACTAGTAGGTGACTT+TGG 0.431001 2_4:+40832693 None:intergenic
TTTATGTCTTGAGAACTTGT+AGG 0.432881 2_4:+40829988 None:intergenic
GAAGTCTAGGCGTTTCTTCA+AGG 0.433072 2_4:+40831500 None:intergenic
CATTCATTTATGCAGGCAGC+TGG 0.444232 2_4:-40830091 Msa0328400:CDS
CGATCTGACATCAGATTTAA+CGG 0.447600 2_4:-40829493 Msa0328400:three_prime_UTR
TTGCTTATCATGATGAAGAA+TGG 0.451345 2_4:-40831984 Msa0328400:CDS
TCATGATAAGCAATATAGAT+AGG 0.456509 2_4:+40831994 None:intergenic
TTGCATGTTGGTTGAAGTAC+TGG 0.458812 2_4:+40832840 None:intergenic
TTTGAATCAAGAGCACTTGA+AGG 0.459539 2_4:+40832363 None:intergenic
CCACATGTTGCTAGAATCAA+TGG 0.460004 2_4:-40832867 Msa0328400:CDS
GCTTACTTGTCATCATGAAC+TGG 0.468369 2_4:+40831955 None:intergenic
GTAGTAGTCTTGTTTGGAAG+TGG 0.468780 2_4:+40832750 None:intergenic
ATGCCATTACAGGTTAAGAA+AGG 0.470141 2_4:-40830277 Msa0328400:intron
ATTGAAATAAGCAAAGTTAG+AGG 0.477981 2_4:-40831295 Msa0328400:CDS
TGAAAAGAGAGAGGAAAATG+AGG 0.479288 2_4:+40833019 None:intergenic
AAGAGAGGGTTTAGATATGT+TGG 0.481696 2_4:-40830127 Msa0328400:CDS
GCTAATGGTCTAGTACTAGT+AGG 0.483572 2_4:+40832684 None:intergenic
AGCTTCATCACAGCCAATTC+AGG 0.489518 2_4:-40832309 Msa0328400:intron
TTATGTCTTGAGAACTTGTA+GGG 0.490548 2_4:+40829989 None:intergenic
TTCTTTCAAGATTTGGAACA+TGG 0.493504 2_4:+40832817 None:intergenic
GAAAATAGAGTTTGTTAAGG+TGG 0.496447 2_4:+40829243 None:intergenic
GAAATGTTGTGAGGGATTGG+AGG 0.499447 2_4:+40833042 None:intergenic
ATCCCACACAGGCTGTTAAA+GGG 0.500349 2_4:+40829289 None:intergenic
AGTGGTTCATTCATTTATGC+AGG 0.501189 2_4:-40830098 Msa0328400:CDS
AGAATTTGATGCAGAAATTG+TGG 0.503076 2_4:-40831365 Msa0328400:CDS
AGCAAAGACATGATTAAGAG+AGG 0.510022 2_4:-40830142 Msa0328400:CDS
CAGAATCAAACCTGAAGTCT+AGG 0.512225 2_4:+40831487 None:intergenic
AAGAAGTGGTGGGAGTGTTA+GGG 0.512402 2_4:-40829417 Msa0328400:three_prime_UTR
AAATGTTGTGAGGGATTGGA+GGG 0.520939 2_4:+40833043 None:intergenic
GTGCTCAAGTTGGATCTGAT+TGG 0.533668 2_4:-40831530 Msa0328400:CDS
TAGGCGTTTCTTCAAGGTGG+AGG 0.543952 2_4:+40831506 None:intergenic
AAATGATCTTGATCTTGAGC+TGG 0.546630 2_4:+40829619 None:intergenic
TACTTGTTGAAGAGTGTGTA+GGG 0.548034 2_4:-40829267 Msa0328400:three_prime_UTR
GTTCCCTTTAACAGCCTGTG+TGG 0.551177 2_4:-40829292 Msa0328400:three_prime_UTR
GAAAAGAGAGAGGAAAATGA+GGG 0.552717 2_4:+40833020 None:intergenic
GGGAATCCGTTAAGAAGTGG+TGG 0.561251 2_4:-40829428 Msa0328400:three_prime_UTR
GCAAAGACATGATTAAGAGA+GGG 0.567917 2_4:-40830141 Msa0328400:CDS
AAAGACTACTACTAATCAAG+AGG 0.568854 2_4:-40832343 Msa0328400:CDS
TAACTGCGTCAAGCTCAAGC+AGG 0.569731 2_4:-40829713 Msa0328400:three_prime_UTR
GCATGTGCAAATAGGAGGCA+AGG 0.570710 2_4:-40829380 Msa0328400:three_prime_UTR
CAAAGTCCTTGTAGGTCACA+TGG 0.570861 2_4:+40829324 None:intergenic
AAGCATAGCTGCTGTTAGAA+GGG 0.572624 2_4:-40832481 Msa0328400:CDS
GCCTGTGGCAAGTGATTAAG+TGG 0.581468 2_4:+40830056 None:intergenic
TCATTTGACAAATACATTTG+GGG 0.586610 2_4:-40830247 Msa0328400:CDS
AGATCAACTTTAAATGATCA+CGG 0.586944 2_4:+40829674 None:intergenic
TGCTGTTAGAAGGGAACAAG+TGG 0.590285 2_4:-40832472 Msa0328400:CDS
TTCCCTTTAACAGCCTGTGT+GGG 0.590416 2_4:-40829291 Msa0328400:three_prime_UTR
CATGTGCATGTGCAAATAGG+AGG 0.591580 2_4:-40829385 Msa0328400:three_prime_UTR
TCTAGCAACATGTGGTGTTG+TGG 0.592032 2_4:+40832875 None:intergenic
GTCTAGGCGTTTCTTCAAGG+TGG 0.595897 2_4:+40831503 None:intergenic
CATTTGACAAATACATTTGG+GGG 0.598419 2_4:-40830246 Msa0328400:CDS
ATAAATGAATGAACCACTGT+TGG 0.599012 2_4:+40830103 None:intergenic
CATGTGCAAATAGGAGGCAA+GGG 0.600226 2_4:-40829379 Msa0328400:three_prime_UTR
AGATTGGAATGAAAAGAGAG+AGG 0.603429 2_4:+40833010 None:intergenic
AAATGAGGGAAATGTTGTGA+GGG 0.604544 2_4:+40833034 None:intergenic
GAGAGACCATGTGACCTACA+AGG 0.606245 2_4:-40829330 Msa0328400:three_prime_UTR
AAGGCTAAAGAAGTAACTGT+AGG 0.608755 2_4:-40832909 Msa0328400:CDS
ACCACTTAATCACTTGCCAC+AGG 0.609569 2_4:-40830057 Msa0328400:CDS
ACAAAACATGTGTAGCTCCA+AGG 0.617528 2_4:-40832928 Msa0328400:exon
CAGGGGAATCCGTTAAGAAG+TGG 0.621578 2_4:-40829431 Msa0328400:three_prime_UTR
TAGATATGTTGGTCCAACAG+TGG 0.633858 2_4:-40830116 Msa0328400:CDS
CCATTGATTCTAGCAACATG+TGG 0.642807 2_4:+40832867 None:intergenic
TTTGGAACATGGTTGCATGT+TGG 0.648256 2_4:+40832828 None:intergenic
TGAAAACTCCAACTTTGGAG+AGG 0.649673 2_4:-40832590 Msa0328400:CDS
AAAATGAGGGAAATGTTGTG+AGG 0.657957 2_4:+40833033 None:intergenic
ATACTTGTTGAAGAGTGTGT+AGG 0.661793 2_4:-40829268 Msa0328400:three_prime_UTR
AGACCATTAGCTATAAAGAG+AGG 0.661843 2_4:-40832672 Msa0328400:CDS
ATGTGCATTGCAAGTGCCTG+TGG 0.666497 2_4:+40830041 None:intergenic
GGAATCCGTTAAGAAGTGGT+GGG 0.668441 2_4:-40829427 Msa0328400:three_prime_UTR
GCTTATCATGATGAAGAATG+GGG 0.675468 2_4:-40831982 Msa0328400:CDS
ATGTTGCTAGAATCAATGGA+AGG 0.678120 2_4:-40832863 Msa0328400:CDS
TCTTTGATCACTGATTCACC+GGG 0.686123 2_4:-40832504 Msa0328400:CDS
TGGCACTTCAACAAGCACAA+AGG 0.702109 2_4:-40832452 Msa0328400:CDS
CTTCAACAAGTATCCCACAC+AGG 0.710515 2_4:+40829278 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTAAAATAATAAGAAG+TGG + chr2_4:40831180-40831199 None:intergenic 10.0%
!! TAAAAAATATTTACCTGAAT+TGG + chr2_4:40829997-40830016 None:intergenic 15.0%
!! TTAAATGTAATTACATGTAT+TGG - chr2_4:40830483-40830502 Msa0328400:intron 15.0%
!!! TTTTTTTTACCTGTAAAATT+TGG + chr2_4:40831039-40831058 None:intergenic 15.0%
!! ATGATTTATATACAAAGTAA+AGG + chr2_4:40831677-40831696 None:intergenic 15.0%
!!! TCTTAAAAAATTGTTTTGAA+TGG - chr2_4:40831956-40831975 Msa0328400:intron 15.0%
!!! AAACTAGTTTTAAATAATCT+TGG + chr2_4:40832774-40832793 None:intergenic 15.0%
!!! CTATTTTCTCAAAATATTAC+TGG + chr2_4:40830458-40830477 None:intergenic 20.0%
!!! TTTGAACTTCTAATTTTAAG+TGG - chr2_4:40830738-40830757 Msa0328400:intron 20.0%
!!! TACATTAATTTTTTTGCTCT+AGG - chr2_4:40830862-40830881 Msa0328400:intron 20.0%
!!! ACATTAATTTTTTTGCTCTA+GGG - chr2_4:40830863-40830882 Msa0328400:intron 20.0%
!!! AACTTATCTACATAAGTTTT+AGG + chr2_4:40831556-40831575 None:intergenic 20.0%
!! AAGTAAAGGATAAATAAACA+AGG + chr2_4:40831663-40831682 None:intergenic 20.0%
!! ATGTATTTACAATTGATCAA+AGG - chr2_4:40831753-40831772 Msa0328400:intron 20.0%
!! TTCATTTGACAAATACATTT+GGG - chr2_4:40832042-40832061 Msa0328400:intron 20.0%
!!! AATTTGTATTGATTTGAGAT+TGG + chr2_4:40832085-40832104 None:intergenic 20.0%
!! ATCTCAAATCAATACAAATT+TGG - chr2_4:40832085-40832104 Msa0328400:intron 20.0%
!! AACAAGAAAAATGTTACTAA+TGG - chr2_4:40832525-40832544 Msa0328400:CDS 20.0%
!! GTTATTAAGAGAAATAGATT+TGG - chr2_4:40832549-40832568 Msa0328400:CDS 20.0%
!! AGATCAAGATCATTTATTAA+AGG - chr2_4:40832679-40832698 Msa0328400:CDS 20.0%
!!! TTGTTAATTTTAAGTTCAAG+CGG + chr2_4:40832751-40832770 None:intergenic 20.0%
!!! AAAGTGTATGAGATTTTTTG+AGG + chr2_4:40829319-40829338 None:intergenic 25.0%
!! GGAGTTTTCATTATGTTTTT+TGG + chr2_4:40829690-40829709 None:intergenic 25.0%
!!! AAGTCAAAGAGTTTTAAAGA+AGG - chr2_4:40829726-40829745 Msa0328400:three_prime_UTR 25.0%
!!! AGTCAAAGAGTTTTAAAGAA+GGG - chr2_4:40829727-40829746 Msa0328400:three_prime_UTR 25.0%
! AAAATGAAGATTGCTCATTA+TGG - chr2_4:40829861-40829880 Msa0328400:three_prime_UTR 25.0%
! TCATGATAAGCAATATAGAT+AGG + chr2_4:40830299-40830318 None:intergenic 25.0%
!! TAGTTTATATGTGTTTCTGA+AGG + chr2_4:40830628-40830647 None:intergenic 25.0%
! TTCAAATAACATCCTGAATA+TGG + chr2_4:40830724-40830743 None:intergenic 25.0%
! ATGTCTATTAGTTCAGAATA+TGG - chr2_4:40830971-40830990 Msa0328400:intron 25.0%
! ATTGAAATAAGCAAAGTTAG+AGG - chr2_4:40830995-40831014 Msa0328400:intron 25.0%
!! TAATGCTAACCAAATTTTAC+AGG - chr2_4:40831027-40831046 Msa0328400:intron 25.0%
! AACAGAAACTTATAAGCTAT+TGG + chr2_4:40831244-40831263 None:intergenic 25.0%
!!! TTGCAGTTAATTTTAACACA+AGG - chr2_4:40831363-40831382 Msa0328400:CDS 25.0%
!!! GTTATTTTCATTAACTATCC+TGG - chr2_4:40831509-40831528 Msa0328400:CDS 25.0%
! GCTTATTTACAATTGATCAA+AGG - chr2_4:40831775-40831794 Msa0328400:intron 25.0%
! GTTCATTTGACAAATACATT+TGG - chr2_4:40832041-40832060 Msa0328400:intron 25.0%
! TCATTTGACAAATACATTTG+GGG - chr2_4:40832043-40832062 Msa0328400:intron 25.0%
! TGTTGTGTATAGTATTAGAT+AGG - chr2_4:40832403-40832422 Msa0328400:CDS 25.0%
! AGATCAACTTTAAATGATCA+CGG + chr2_4:40832619-40832638 None:intergenic 25.0%
AGGAGTAATAGTGTTAAGAT+TGG + chr2_4:40829299-40829318 None:intergenic 30.0%
! TTCTTTCAAGATTTGGAACA+TGG + chr2_4:40829476-40829495 None:intergenic 30.0%
GAGTTTCTTCTTTCAAGATT+TGG + chr2_4:40829483-40829502 None:intergenic 30.0%
TTTCCTCTCTTTATAGCTAA+TGG + chr2_4:40829624-40829643 None:intergenic 30.0%
AAGAGAGGAAATGACAATAA+TGG - chr2_4:40829633-40829652 Msa0328400:three_prime_UTR 30.0%
CATAATGAAAACTCCAACTT+TGG - chr2_4:40829695-40829714 Msa0328400:three_prime_UTR 30.0%
!!! AGTTTTAAAGAAGGGTCTTT+TGG - chr2_4:40829735-40829754 Msa0328400:three_prime_UTR 30.0%
AAAGACTACTACTAATCAAG+AGG - chr2_4:40829947-40829966 Msa0328400:three_prime_UTR 30.0%
!! ACATGTGTGACTTTAGAATT+AGG + chr2_4:40830074-40830093 None:intergenic 30.0%
TTGCTTATCATGATGAAGAA+TGG - chr2_4:40830306-40830325 Msa0328400:intron 30.0%
TGCTTATCATGATGAAGAAT+GGG - chr2_4:40830307-40830326 Msa0328400:intron 30.0%
TATGTTACACACAACTATGA+AGG - chr2_4:40830372-40830391 Msa0328400:intron 30.0%
TATTGAGATGTGTCAAACAT+CGG - chr2_4:40830524-40830543 Msa0328400:intron 30.0%
ATGCCTTCAATCTCAAATAT+TGG - chr2_4:40830549-40830568 Msa0328400:intron 30.0%
! ACACCAATATTTGAGATTGA+AGG + chr2_4:40830555-40830574 None:intergenic 30.0%
AGAATTTGATGCAGAAATTG+TGG - chr2_4:40830925-40830944 Msa0328400:intron 30.0%
CATTTGCTTATCAGTCAAAT+TGG + chr2_4:40830951-40830970 None:intergenic 30.0%
ATTGATCAATCAACTCATGT+GGG + chr2_4:40831273-40831292 None:intergenic 30.0%
!! AATATGTGACAGCATTGATA+AGG + chr2_4:40831309-40831328 None:intergenic 30.0%
ATTCAGCTGTTTATCAAGAT+AGG + chr2_4:40831391-40831410 None:intergenic 30.0%
TATCTTGATAAACAGCTGAA+TGG - chr2_4:40831390-40831409 Msa0328400:intron 30.0%
CACTAATCAACGTATTTAGT+CGG - chr2_4:40831595-40831614 Msa0328400:intron 30.0%
AGGCTTATATACAAAGACAA+AGG - chr2_4:40831795-40831814 Msa0328400:intron 30.0%
!! CTTTGAAATTATGCCATTAC+AGG - chr2_4:40832003-40832022 Msa0328400:intron 30.0%
CATTTGACAAATACATTTGG+GGG - chr2_4:40832044-40832063 Msa0328400:intron 30.0%
ATAAATGAATGAACCACTGT+TGG + chr2_4:40832190-40832209 None:intergenic 30.0%
TGATTAAGTGGTCATTTGTT+AGG + chr2_4:40832225-40832244 None:intergenic 30.0%
GGGTCAATTAATCTCTTATA+TGG + chr2_4:40832284-40832303 None:intergenic 30.0%
TTATGTCTTGAGAACTTGTA+GGG + chr2_4:40832304-40832323 None:intergenic 30.0%
TTTATGTCTTGAGAACTTGT+AGG + chr2_4:40832305-40832324 None:intergenic 30.0%
! TATGCTGACTATAGTTTACT+TGG - chr2_4:40832373-40832392 Msa0328400:CDS 30.0%
AAATAATGCAAAGTCCTTGT+AGG + chr2_4:40832977-40832996 None:intergenic 30.0%
AAATGAGGGAAATGTTGTGA+GGG + chr2_4:40829259-40829278 None:intergenic 35.0%
AAAATGAGGGAAATGTTGTG+AGG + chr2_4:40829260-40829279 None:intergenic 35.0%
GAAAAGAGAGAGGAAAATGA+GGG + chr2_4:40829273-40829292 None:intergenic 35.0%
TGAAAAGAGAGAGGAAAATG+AGG + chr2_4:40829274-40829293 None:intergenic 35.0%
AGATTGGAATGAAAAGAGAG+AGG + chr2_4:40829283-40829302 None:intergenic 35.0%
TACAGTTACTTCTTTAGCCT+TGG + chr2_4:40829382-40829401 None:intergenic 35.0%
AAGGCTAAAGAAGTAACTGT+AGG - chr2_4:40829381-40829400 Msa0328400:three_prime_UTR 35.0%
ATGTTGCTAGAATCAATGGA+AGG - chr2_4:40829427-40829446 Msa0328400:three_prime_UTR 35.0%
!!! GGTAATGGAGAAAGTGATTT+TGG + chr2_4:40829522-40829541 None:intergenic 35.0%
!! GAAGTAGTAGTAGTCTTGTT+TGG + chr2_4:40829549-40829568 None:intergenic 35.0%
!!! TTTGGTTTTGGAGAAATTGG+TGG + chr2_4:40829582-40829601 None:intergenic 35.0%
!!! GACTTTGGTTTTGGAGAAAT+TGG + chr2_4:40829585-40829604 None:intergenic 35.0%
AGACCATTAGCTATAAAGAG+AGG - chr2_4:40829618-40829637 Msa0328400:three_prime_UTR 35.0%
!! TTCTTTGATCACTGATTCAC+CGG - chr2_4:40829785-40829804 Msa0328400:three_prime_UTR 35.0%
!! TTTGAATCAAGAGCACTTGA+AGG + chr2_4:40829930-40829949 None:intergenic 35.0%
ATTGCACTCGGTATCAAAAA+AGG + chr2_4:40830221-40830240 None:intergenic 35.0%
AGCTAAAGCAAAATTGCACT+CGG + chr2_4:40830233-40830252 None:intergenic 35.0%
GCTTATCATGATGAAGAATG+GGG - chr2_4:40830308-40830327 Msa0328400:intron 35.0%
GTAACATAGAGAGAGAAGTA+GGG + chr2_4:40830360-40830379 None:intergenic 35.0%
TGTAACATAGAGAGAGAAGT+AGG + chr2_4:40830361-40830380 None:intergenic 35.0%
!! TATTGGTGTCATCTTTGTGT+TGG - chr2_4:40830500-40830519 Msa0328400:intron 35.0%
ATGATGTTGTGTCCATATTC+AGG - chr2_4:40830709-40830728 Msa0328400:intron 35.0%
!! ATTTTAAGTGGTGCTCAAGT+TGG - chr2_4:40830750-40830769 Msa0328400:intron 35.0%
AATTCACTCGTTGCCAAAAA+AGG - chr2_4:40831201-40831220 Msa0328400:intron 35.0%
TTGATCAATCAACTCATGTG+GGG + chr2_4:40831272-40831291 None:intergenic 35.0%
CATTGATCAATCAACTCATG+TGG + chr2_4:40831274-40831293 None:intergenic 35.0%
! CTCTTATTTACATGAGTTCC+AGG + chr2_4:40831530-40831549 None:intergenic 35.0%
!! TAGTGAGTGTTGATAGCTTA+TGG + chr2_4:40831580-40831599 None:intergenic 35.0%
!! AATTAGAGCATTGAGAAGTC+AGG + chr2_4:40831851-40831870 None:intergenic 35.0%
ATGCCATTACAGGTTAAGAA+AGG - chr2_4:40832013-40832032 Msa0328400:intron 35.0%
AAGCCTTTCTTAACCTGTAA+TGG + chr2_4:40832019-40832038 None:intergenic 35.0%
TTACAGGTTAAGAAAGGCTT+TGG - chr2_4:40832019-40832038 Msa0328400:intron 35.0%
!! TCTGATTTTGATGTCTTCAC+TGG + chr2_4:40832121-40832140 None:intergenic 35.0%
AGCAAAGACATGATTAAGAG+AGG - chr2_4:40832148-40832167 Msa0328400:intron 35.0%
GCAAAGACATGATTAAGAGA+GGG - chr2_4:40832149-40832168 Msa0328400:intron 35.0%
AAGAGAGGGTTTAGATATGT+TGG - chr2_4:40832163-40832182 Msa0328400:intron 35.0%
AGTGGTTCATTCATTTATGC+AGG - chr2_4:40832192-40832211 Msa0328400:intron 35.0%
AAATGATCTTGATCTTGAGC+TGG + chr2_4:40832674-40832693 None:intergenic 35.0%
CGATCTGACATCAGATTTAA+CGG - chr2_4:40832797-40832816 Msa0328400:CDS 35.0%
! ATACTTGTTGAAGAGTGTGT+AGG - chr2_4:40833022-40833041 Msa0328400:five_prime_UTR 35.0%
! TACTTGTTGAAGAGTGTGTA+GGG - chr2_4:40833023-40833042 Msa0328400:five_prime_UTR 35.0%
AAATGTTGTGAGGGATTGGA+GGG + chr2_4:40829250-40829269 None:intergenic 40.0%
ACAAAACATGTGTAGCTCCA+AGG - chr2_4:40829362-40829381 Msa0328400:three_prime_UTR 40.0%
!! CCATTGATTCTAGCAACATG+TGG + chr2_4:40829426-40829445 None:intergenic 40.0%
CCACATGTTGCTAGAATCAA+TGG - chr2_4:40829423-40829442 Msa0328400:three_prime_UTR 40.0%
! TTGCATGTTGGTTGAAGTAC+TGG + chr2_4:40829453-40829472 None:intergenic 40.0%
! TTTGGAACATGGTTGCATGT+TGG + chr2_4:40829465-40829484 None:intergenic 40.0%
!! GTCTTGTTTGGAAGTGGTAA+TGG + chr2_4:40829537-40829556 None:intergenic 40.0%
!! GTAGTAGTCTTGTTTGGAAG+TGG + chr2_4:40829543-40829562 None:intergenic 40.0%
!! CTAGTAGGTGACTTTGGTTT+TGG + chr2_4:40829594-40829613 None:intergenic 40.0%
CTAGTACTAGTAGGTGACTT+TGG + chr2_4:40829600-40829619 None:intergenic 40.0%
GCTAATGGTCTAGTACTAGT+AGG + chr2_4:40829609-40829628 None:intergenic 40.0%
! TGAAAACTCCAACTTTGGAG+AGG - chr2_4:40829700-40829719 Msa0328400:three_prime_UTR 40.0%
!! GACTTTTTCCTCTCCAAAGT+TGG + chr2_4:40829711-40829730 None:intergenic 40.0%
!! TCTTTGATCACTGATTCACC+GGG - chr2_4:40829786-40829805 Msa0328400:three_prime_UTR 40.0%
AAGCATAGCTGCTGTTAGAA+GGG - chr2_4:40829809-40829828 Msa0328400:three_prime_UTR 40.0%
! AGAGCACTTGAAGGATCAAT+AGG + chr2_4:40829921-40829940 None:intergenic 40.0%
GCTTACTTGTCATCATGAAC+TGG + chr2_4:40830338-40830357 None:intergenic 40.0%
!! ATTGGTGTCGAATACTGACA+CGG - chr2_4:40830567-40830586 Msa0328400:intron 40.0%
CAGAATCAAACCTGAAGTCT+AGG + chr2_4:40830806-40830825 None:intergenic 40.0%
!!! AACTTGTCTGCAGCCTTTTT+TGG + chr2_4:40831217-40831236 None:intergenic 40.0%
GCATTGAGAAGTCAGGATAA+TGG + chr2_4:40831844-40831863 None:intergenic 40.0%
AAACACCTGCGTTATTCGTA+AGG + chr2_4:40831911-40831930 None:intergenic 40.0%
TAGATATGTTGGTCCAACAG+TGG - chr2_4:40832174-40832193 Msa0328400:intron 40.0%
GCACTTGCAATGCACATTAT+TGG - chr2_4:40832255-40832274 Msa0328400:intron 40.0%
!!! TAGAAGCGTGCTTTTTCAAC+AGG - chr2_4:40832840-40832859 Msa0328400:CDS 40.0%
!!! AGAAGCGTGCTTTTTCAACA+GGG - chr2_4:40832841-40832860 Msa0328400:CDS 40.0%
GAAATGTTGTGAGGGATTGG+AGG + chr2_4:40829251-40829270 None:intergenic 45.0%
AGGGAAATGTTGTGAGGGAT+TGG + chr2_4:40829254-40829273 None:intergenic 45.0%
! TCTAGCAACATGTGGTGTTG+TGG + chr2_4:40829418-40829437 None:intergenic 45.0%
GAAGCATAGCTGCTGTTAGA+AGG - chr2_4:40829808-40829827 Msa0328400:three_prime_UTR 45.0%
TGCTGTTAGAAGGGAACAAG+TGG - chr2_4:40829818-40829837 Msa0328400:three_prime_UTR 45.0%
TGGCACTTCAACAAGCACAA+AGG - chr2_4:40829838-40829857 Msa0328400:three_prime_UTR 45.0%
AGCTTCATCACAGCCAATTC+AGG - chr2_4:40829981-40830000 Msa0328400:three_prime_UTR 45.0%
AGGACACAACTATGAAGCAC+CGG - chr2_4:40830392-40830411 Msa0328400:intron 45.0%
TCTGATGTATGTGCATGTGC+CGG + chr2_4:40830414-40830433 None:intergenic 45.0%
!! GTGCTCAAGTTGGATCTGAT+TGG - chr2_4:40830760-40830779 Msa0328400:intron 45.0%
GAAGTCTAGGCGTTTCTTCA+AGG + chr2_4:40830793-40830812 None:intergenic 45.0%
TGAAGAAACGCCTAGACTTC+AGG - chr2_4:40830793-40830812 Msa0328400:intron 45.0%
!! CGGTTAAAGCATCGATGAGT+CGG - chr2_4:40831615-40831634 Msa0328400:intron 45.0%
!! TAGCCAGTGAGTGTTGATAC+AGG - chr2_4:40831881-40831900 Msa0328400:intron 45.0%
TAGCCTGTATCAACACTCAC+TGG + chr2_4:40831887-40831906 None:intergenic 45.0%
GCTATCCTTACGAATAACGC+AGG - chr2_4:40831903-40831922 Msa0328400:intron 45.0%
CATTCATTTATGCAGGCAGC+TGG - chr2_4:40832199-40832218 Msa0328400:intron 45.0%
ACCACTTAATCACTTGCCAC+AGG - chr2_4:40832233-40832252 Msa0328400:intron 45.0%
!!! GAAGCGTGCTTTTTCAACAG+GGG - chr2_4:40832842-40832861 Msa0328400:CDS 45.0%
GGAATCCGTTAAGAAGTGGT+GGG - chr2_4:40832863-40832882 Msa0328400:CDS 45.0%
ACACTCCCACCACTTCTTAA+CGG + chr2_4:40832871-40832890 None:intergenic 45.0%
!! TAAGAAGTGGTGGGAGTGTT+AGG - chr2_4:40832872-40832891 Msa0328400:CDS 45.0%
!! AAGAAGTGGTGGGAGTGTTA+GGG - chr2_4:40832873-40832892 Msa0328400:CDS 45.0%
CAGCATGTGCATGTGCAAAT+AGG - chr2_4:40832902-40832921 Msa0328400:CDS 45.0%
CATGTGCATGTGCAAATAGG+AGG - chr2_4:40832905-40832924 Msa0328400:CDS 45.0%
CATGTGCAAATAGGAGGCAA+GGG - chr2_4:40832911-40832930 Msa0328400:CDS 45.0%
CAAAGTCCTTGTAGGTCACA+TGG + chr2_4:40832969-40832988 None:intergenic 45.0%
TTCCCTTTAACAGCCTGTGT+GGG - chr2_4:40832999-40833018 Msa0328400:five_prime_UTR 45.0%
ATCCCACACAGGCTGTTAAA+GGG + chr2_4:40833004-40833023 None:intergenic 45.0%
TATCCCACACAGGCTGTTAA+AGG + chr2_4:40833005-40833024 None:intergenic 45.0%
CTTCAACAAGTATCCCACAC+AGG + chr2_4:40833015-40833034 None:intergenic 45.0%
CTAACAGCAGCTATGCTTCC+CGG + chr2_4:40829807-40829826 None:intergenic 50.0%
TAGGCGTTTCTTCAAGGTGG+AGG + chr2_4:40830787-40830806 None:intergenic 50.0%
GTCTAGGCGTTTCTTCAAGG+TGG + chr2_4:40830790-40830809 None:intergenic 50.0%
GCCTGTGGCAAGTGATTAAG+TGG + chr2_4:40832237-40832256 None:intergenic 50.0%
ATGTGCATTGCAAGTGCCTG+TGG + chr2_4:40832252-40832271 None:intergenic 50.0%
TAACTGCGTCAAGCTCAAGC+AGG - chr2_4:40832577-40832596 Msa0328400:CDS 50.0%
CAGGGGAATCCGTTAAGAAG+TGG - chr2_4:40832859-40832878 Msa0328400:CDS 50.0%
GGGAATCCGTTAAGAAGTGG+TGG - chr2_4:40832862-40832881 Msa0328400:CDS 50.0%
GCATGTGCAAATAGGAGGCA+AGG - chr2_4:40832910-40832929 Msa0328400:CDS 50.0%
GAGAGACCATGTGACCTACA+AGG - chr2_4:40832960-40832979 Msa0328400:five_prime_UTR 50.0%
GTTCCCTTTAACAGCCTGTG+TGG - chr2_4:40832998-40833017 Msa0328400:five_prime_UTR 50.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 40829247 40833065 40829247 ID=Msa0328400;Name=Msa0328400
chr2_4 mRNA 40829247 40833065 40829247 ID=Msa0328400-mRNA-1;Parent=Msa0328400;Name=Msa0328400-mRNA-1;_AED=0.02;_eAED=0.02;_QI=122|1|1|1|1|1|5|777|391
chr2_4 exon 40829247 40830287 40829247 ID=Msa0328400-mRNA-1:exon:12467;Parent=Msa0328400-mRNA-1
chr2_4 exon 40831264 40831399 40831264 ID=Msa0328400-mRNA-1:exon:12466;Parent=Msa0328400-mRNA-1
chr2_4 exon 40831498 40831581 40831498 ID=Msa0328400-mRNA-1:exon:12465;Parent=Msa0328400-mRNA-1
chr2_4 exon 40831961 40832018 40831961 ID=Msa0328400-mRNA-1:exon:12464;Parent=Msa0328400-mRNA-1
chr2_4 exon 40832310 40833065 40832310 ID=Msa0328400-mRNA-1:exon:12463;Parent=Msa0328400-mRNA-1
chr2_4 five_prime_UTR 40832944 40833065 40832944 ID=Msa0328400-mRNA-1:five_prime_utr;Parent=Msa0328400-mRNA-1
chr2_4 CDS 40832310 40832943 40832310 ID=Msa0328400-mRNA-1:cds;Parent=Msa0328400-mRNA-1
chr2_4 CDS 40831961 40832018 40831961 ID=Msa0328400-mRNA-1:cds;Parent=Msa0328400-mRNA-1
chr2_4 CDS 40831498 40831581 40831498 ID=Msa0328400-mRNA-1:cds;Parent=Msa0328400-mRNA-1
chr2_4 CDS 40831264 40831399 40831264 ID=Msa0328400-mRNA-1:cds;Parent=Msa0328400-mRNA-1
chr2_4 CDS 40830024 40830287 40830024 ID=Msa0328400-mRNA-1:cds;Parent=Msa0328400-mRNA-1
chr2_4 three_prime_UTR 40829247 40830023 40829247 ID=Msa0328400-mRNA-1:three_prime_utr;Parent=Msa0328400-mRNA-1
Gene Sequence

>Msa0328400

ATGTGTAGCTCCAAGGCTAAAGAAGTAACTGTAGGCATAGAATCTTCCACAACACCACATGTTGCTAGAATCAATGGAAGGCCAGTACTTCAACCAACATGCAACCATGTTCCAAATCTTGAAAGAAGAAACTCAATTAAGAAATCAACACCAAAATCACTTTCTCCATTACCACTTCCAAACAAGACTACTACTACTTCTTCATTGACACCACCAATTTCTCCAAAACCAAAGTCACCTACTAGTACTAGACCATTAGCTATAAAGAGAGGAAATGACAATAATGGACTTAATTTAAGCTGTGAAAAGATTTCAATACCAAAAAACATAATGAAAACTCCAACTTTGGAGAGGAAAAAGTCAAAGAGTTTTAAAGAAGGGTCTTTTGGTATTGAAGCTTCGTTGAGTTATTCTTCTTCTTTGATCACTGATTCACCGGGAAGCATAGCTGCTGTTAGAAGGGAACAAGTGGCACTTCAACAAGCACAAAGGAAAATGAAGATTGCTCATTATGGAAGATCAAAGTCTGCTAAATTTGAAAGAGTTTTTCCTATTGATCCTTCAAGTGCTCTTGATTCAAAGACTACTACTAATCAAGAGGAGAAGAGATGCAGCTTCATCACAGCCAATTCAGATCCTATCTATATTGCTTATCATGATGAAGAATGGGGAGTTCCAGTTCATGATGACAAGATGTTATTTGAACTTCTAATTTTAAGTGGTGCTCAAGTTGGATCTGATTGGACCTCCACCTTGAAGAAACGCCTAGACTTCAGAGCTGCATTTTCAGAATTTGATGCAGAAATTGTGGCCAATTTGACTGATAAGCAAATGATGTCTATTAGTTCAGAATATGGCATTGAAATAAGCAAAGTTAGAGGAGTTGTTGATAATGCTAACCAAATTTTACAGGTTAAGAAAGGCTTTGGTTCATTTGACAAATACATTTGGGGGTTTGTGAATCATAAACCAATCTCAAATCAATACAAATTTGGTCACAAGATTCCAGTGAAGACATCAAAATCAGAGAGCATAAGCAAAGACATGATTAAGAGAGGGTTTAGATATGTTGGTCCAACAGTGGTTCATTCATTTATGCAGGCAGCTGGCCTAACAAATGACCACTTAATCACTTGCCACAGGCACTTGCAATGCACATTATTGGCAGCCATATAA
Protein sequence

>Msa0328400

MCSSKAKEVTVGIESSTTPHVARINGRPVLQPTCNHVPNLERRNSIKKSTPKSLSPLPLPNKTTTTSSLTPPISPKPKSPTSTRPLAIKRGNDNNGLNLSCEKISIPKNIMKTPTLERKKSKSFKEGSFGIEASLSYSSSLITDSPGSIAAVRREQVALQQAQRKMKIAHYGRSKSAKFERVFPIDPSSALDSKTTTNQEEKRCSFITANSDPIYIAYHDEEWGVPVHDDKMLFELLILSGAQVGSDWTSTLKKRLDFRAAFSEFDAEIVANLTDKQMMSISSEYGIEISKVRGVVDNANQILQVKKGFGSFDKYIWGFVNHKPISNQYKFGHKIPVKTSKSESISKDMIKRGFRYVGPTVVHSFMQAAGLTNDHLITCHRHLQCTLLAAI*