AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0328410

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0328410 MtrunA17_Chr2g0309681 98.214 392 4 3 1 391 1 390 0.0 786
Msa0328410 MtrunA17_Chr8g0356301 77.215 395 73 8 1 390 1 383 0.0 568
Msa0328410 MtrunA17_Chr4g0073991 51.813 193 91 1 199 389 145 337 1.79e-69 223
Msa0328410 MtrunA17_Chr5g0420791 48.958 192 96 1 200 389 110 301 1.21e-68 219
Msa0328410 MtrunA17_Chr8g0366071 48.500 200 98 2 195 389 109 308 7.06e-68 217
Msa0328410 MtrunA17_Chr4g0001371 49.738 191 94 1 199 387 134 324 3.02e-67 217
Msa0328410 MtrunA17_Chr3g0141411 45.495 222 116 3 169 387 97 316 7.56e-65 210
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0328410 AT3G12710.1 70.609 279 63 4 115 389 46 309 3.04e-139 399
Msa0328410 AT5G44680.1 51.392 395 146 8 1 391 1 353 3.33e-135 391
Msa0328410 AT1G75090.1 53.723 188 85 1 202 387 119 306 4.80e-74 234
Msa0328410 AT5G57970.1 50.000 212 99 2 183 387 130 341 1.31e-72 231
Msa0328410 AT5G57970.2 50.000 212 99 2 183 387 130 341 1.31e-72 231
Msa0328410 AT1G80850.1 54.839 186 82 1 202 385 134 319 3.42e-71 226
Msa0328410 AT1G13635.2 50.510 196 95 1 196 389 109 304 3.56e-71 226
Msa0328410 AT1G13635.1 50.510 196 95 1 196 389 109 304 3.56e-71 226
Msa0328410 AT1G15970.1 50.769 195 84 2 202 389 145 334 8.92e-67 216
Msa0328410 AT1G15970.2 50.769 195 84 2 202 389 145 334 8.92e-67 216

Find 112 sgRNAs with CRISPR-Local

Find 181 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCACTTGCAATGCACATTAT+TGG 0.261232 2_4:+40848438 Msa0328410:CDS
CATTTGCTTATCAGTCAAAT+TGG 0.271540 2_4:-40847131 None:intergenic
TTCATTTGACAAATACATTT+GGG 0.277104 2_4:+40848225 Msa0328410:CDS
TATCCCACACAGGCTGTTAA+AGG 0.287777 2_4:-40849185 None:intergenic
AGATCAAGATCATTTATTAA+AGG 0.297955 2_4:+40848862 Msa0328410:three_prime_UTR
TAAGAAGTGGTGGGAGTGTT+AGG 0.297982 2_4:+40849055 Msa0328410:three_prime_UTR
GGTAATGGAGAAAGTGATTT+TGG 0.299287 2_4:-40845702 None:intergenic
ATCTCAAATCAATACAAATT+TGG 0.303182 2_4:+40848268 Msa0328410:CDS
TTTCCTCTCTTTATAGCTAA+TGG 0.311064 2_4:-40845804 None:intergenic
CTAGTAGGTGACTTTGGTTT+TGG 0.311738 2_4:-40845774 None:intergenic
AAAATGAAGATTGCTCATTA+TGG 0.312266 2_4:+40846044 Msa0328410:CDS
GAGTTTCTTCTTTCAAGATT+TGG 0.322751 2_4:-40845663 None:intergenic
CATAATGAAAACTCCAACTT+TGG 0.328226 2_4:+40845878 Msa0328410:CDS
GGGTCAATTAATCTCTTATA+TGG 0.340630 2_4:-40848464 None:intergenic
GTTCATTTGACAAATACATT+TGG 0.343231 2_4:+40848224 Msa0328410:CDS
TGATTAAGTGGTCATTTGTT+AGG 0.343650 2_4:-40848405 None:intergenic
GAAGCATAGCTGCTGTTAGA+AGG 0.354512 2_4:+40845991 Msa0328410:CDS
TACAGTTACTTCTTTAGCCT+TGG 0.358427 2_4:-40845562 None:intergenic
ATGTCTATTAGTTCAGAATA+TGG 0.363323 2_4:+40847154 Msa0328410:CDS
GAAGTAGTAGTAGTCTTGTT+TGG 0.369631 2_4:-40845729 None:intergenic
TGAAGAAACGCCTAGACTTC+AGG 0.370308 2_4:+40846976 Msa0328410:CDS
AAGAGAGGAAATGACAATAA+TGG 0.373376 2_4:+40845816 Msa0328410:CDS
CAGCATGTGCATGTGCAAAT+AGG 0.375156 2_4:+40849085 Msa0328410:three_prime_UTR
ACACTCCCACCACTTCTTAA+CGG 0.386475 2_4:-40849051 None:intergenic
TGTTGTGTATAGTATTAGAT+AGG 0.387658 2_4:+40848586 Msa0328410:three_prime_UTR
AGGGAAATGTTGTGAGGGAT+TGG 0.388202 2_4:-40845434 None:intergenic
AGGAGTAATAGTGTTAAGAT+TGG 0.390149 2_4:-40845479 None:intergenic
CTAACAGCAGCTATGCTTCC+CGG 0.396009 2_4:-40845987 None:intergenic
TGCTTATCATGATGAAGAAT+GGG 0.396248 2_4:+40846490 Msa0328410:CDS
GTCTTGTTTGGAAGTGGTAA+TGG 0.397911 2_4:-40845717 None:intergenic
AAAGAAAATAGAGTTTGTTA+AGG 0.398022 2_4:-40849233 None:intergenic
AAATAATGCAAAGTCCTTGT+AGG 0.402213 2_4:-40849157 None:intergenic
TTACAGGTTAAGAAAGGCTT+TGG 0.404283 2_4:+40848202 Msa0328410:intron
TTCTTTGATCACTGATTCAC+CGG 0.407327 2_4:+40845968 Msa0328410:CDS
AGAGCACTTGAAGGATCAAT+AGG 0.410745 2_4:-40846101 None:intergenic
AATTTGTATTGATTTGAGAT+TGG 0.419291 2_4:-40848265 None:intergenic
TATGCTGACTATAGTTTACT+TGG 0.422375 2_4:+40848556 Msa0328410:three_prime_UTR
GTTATTAAGAGAAATAGATT+TGG 0.426782 2_4:+40848732 Msa0328410:three_prime_UTR
CTAGTACTAGTAGGTGACTT+TGG 0.431001 2_4:-40845780 None:intergenic
TTTATGTCTTGAGAACTTGT+AGG 0.432881 2_4:-40848485 None:intergenic
GAAGTCTAGGCGTTTCTTCA+AGG 0.433072 2_4:-40846973 None:intergenic
CATTCATTTATGCAGGCAGC+TGG 0.444232 2_4:+40848382 Msa0328410:CDS
CGATCTGACATCAGATTTAA+CGG 0.447600 2_4:+40848980 Msa0328410:three_prime_UTR
TTGCTTATCATGATGAAGAA+TGG 0.451345 2_4:+40846489 Msa0328410:CDS
TCATGATAAGCAATATAGAT+AGG 0.456509 2_4:-40846479 None:intergenic
TTGCATGTTGGTTGAAGTAC+TGG 0.458812 2_4:-40845633 None:intergenic
TTTGAATCAAGAGCACTTGA+AGG 0.459539 2_4:-40846110 None:intergenic
CCACATGTTGCTAGAATCAA+TGG 0.460004 2_4:+40845606 Msa0328410:CDS
GCTTACTTGTCATCATGAAC+TGG 0.468369 2_4:-40846518 None:intergenic
GTAGTAGTCTTGTTTGGAAG+TGG 0.468780 2_4:-40845723 None:intergenic
ATGCCATTACAGGTTAAGAA+AGG 0.470141 2_4:+40848196 Msa0328410:intron
ATTGAAATAAGCAAAGTTAG+AGG 0.477981 2_4:+40847178 Msa0328410:CDS
TGAAAAGAGAGAGGAAAATG+AGG 0.479288 2_4:-40845454 None:intergenic
AAGAGAGGGTTTAGATATGT+TGG 0.481696 2_4:+40848346 Msa0328410:CDS
GCTAATGGTCTAGTACTAGT+AGG 0.483572 2_4:-40845789 None:intergenic
AGCTTCATCACAGCCAATTC+AGG 0.489518 2_4:+40846164 Msa0328410:CDS
TTATGTCTTGAGAACTTGTA+GGG 0.490548 2_4:-40848484 None:intergenic
TTCTTTCAAGATTTGGAACA+TGG 0.493504 2_4:-40845656 None:intergenic
GAAAATAGAGTTTGTTAAGG+TGG 0.496447 2_4:-40849230 None:intergenic
GAAATGTTGTGAGGGATTGG+AGG 0.499447 2_4:-40845431 None:intergenic
ATCCCACACAGGCTGTTAAA+GGG 0.500349 2_4:-40849184 None:intergenic
AGTGGTTCATTCATTTATGC+AGG 0.501189 2_4:+40848375 Msa0328410:CDS
AGAATTTGATGCAGAAATTG+TGG 0.503076 2_4:+40847108 Msa0328410:CDS
AGCAAAGACATGATTAAGAG+AGG 0.510022 2_4:+40848331 Msa0328410:CDS
CAGAATCAAACCTGAAGTCT+AGG 0.512225 2_4:-40846986 None:intergenic
AAGAAGTGGTGGGAGTGTTA+GGG 0.512402 2_4:+40849056 Msa0328410:three_prime_UTR
AAATGTTGTGAGGGATTGGA+GGG 0.520939 2_4:-40845430 None:intergenic
GTGCTCAAGTTGGATCTGAT+TGG 0.533668 2_4:+40846943 Msa0328410:CDS
TAGGCGTTTCTTCAAGGTGG+AGG 0.543952 2_4:-40846967 None:intergenic
AAATGATCTTGATCTTGAGC+TGG 0.546630 2_4:-40848854 None:intergenic
TACTTGTTGAAGAGTGTGTA+GGG 0.548034 2_4:+40849206 Msa0328410:three_prime_UTR
GTTCCCTTTAACAGCCTGTG+TGG 0.551177 2_4:+40849181 Msa0328410:three_prime_UTR
GAAAAGAGAGAGGAAAATGA+GGG 0.552717 2_4:-40845453 None:intergenic
GGGAATCCGTTAAGAAGTGG+TGG 0.561251 2_4:+40849045 Msa0328410:three_prime_UTR
GCAAAGACATGATTAAGAGA+GGG 0.567917 2_4:+40848332 Msa0328410:CDS
AAAGACTACTACTAATCAAG+AGG 0.568854 2_4:+40846130 Msa0328410:CDS
TAACTGCGTCAAGCTCAAGC+AGG 0.569731 2_4:+40848760 Msa0328410:three_prime_UTR
GCATGTGCAAATAGGAGGCA+AGG 0.570710 2_4:+40849093 Msa0328410:three_prime_UTR
CAAAGTCCTTGTAGGTCACA+TGG 0.570861 2_4:-40849149 None:intergenic
AAGCATAGCTGCTGTTAGAA+GGG 0.572624 2_4:+40845992 Msa0328410:CDS
GCCTGTGGCAAGTGATTAAG+TGG 0.581468 2_4:-40848417 None:intergenic
TCATTTGACAAATACATTTG+GGG 0.586610 2_4:+40848226 Msa0328410:CDS
AGATCAACTTTAAATGATCA+CGG 0.586944 2_4:-40848799 None:intergenic
TGCTGTTAGAAGGGAACAAG+TGG 0.590285 2_4:+40846001 Msa0328410:CDS
TTCCCTTTAACAGCCTGTGT+GGG 0.590416 2_4:+40849182 Msa0328410:three_prime_UTR
CATGTGCATGTGCAAATAGG+AGG 0.591580 2_4:+40849088 Msa0328410:three_prime_UTR
TCTAGCAACATGTGGTGTTG+TGG 0.592032 2_4:-40845598 None:intergenic
GTCTAGGCGTTTCTTCAAGG+TGG 0.595897 2_4:-40846970 None:intergenic
CATTTGACAAATACATTTGG+GGG 0.598419 2_4:+40848227 Msa0328410:CDS
ATAAATGAATGAACCACTGT+TGG 0.599012 2_4:-40848370 None:intergenic
CATGTGCAAATAGGAGGCAA+GGG 0.600226 2_4:+40849094 Msa0328410:three_prime_UTR
AGATTGGAATGAAAAGAGAG+AGG 0.603429 2_4:-40845463 None:intergenic
AAATGAGGGAAATGTTGTGA+GGG 0.604544 2_4:-40845439 None:intergenic
GAGAGACCATGTGACCTACA+AGG 0.606245 2_4:+40849143 Msa0328410:three_prime_UTR
AAGGCTAAAGAAGTAACTGT+AGG 0.608755 2_4:+40845564 Msa0328410:CDS
ACCACTTAATCACTTGCCAC+AGG 0.609569 2_4:+40848416 Msa0328410:CDS
ACAAAACATGTGTAGCTCCA+AGG 0.617528 2_4:+40845545 Msa0328410:exon
CAGGGGAATCCGTTAAGAAG+TGG 0.621578 2_4:+40849042 Msa0328410:three_prime_UTR
TAGATATGTTGGTCCAACAG+TGG 0.633858 2_4:+40848357 Msa0328410:CDS
CCATTGATTCTAGCAACATG+TGG 0.642807 2_4:-40845606 None:intergenic
TTTGGAACATGGTTGCATGT+TGG 0.648256 2_4:-40845645 None:intergenic
TGAAAACTCCAACTTTGGAG+AGG 0.649673 2_4:+40845883 Msa0328410:CDS
AAAATGAGGGAAATGTTGTG+AGG 0.657957 2_4:-40845440 None:intergenic
ATACTTGTTGAAGAGTGTGT+AGG 0.661793 2_4:+40849205 Msa0328410:three_prime_UTR
AGACCATTAGCTATAAAGAG+AGG 0.661843 2_4:+40845801 Msa0328410:CDS
ATGTGCATTGCAAGTGCCTG+TGG 0.666497 2_4:-40848432 None:intergenic
GGAATCCGTTAAGAAGTGGT+GGG 0.668441 2_4:+40849046 Msa0328410:three_prime_UTR
GCTTATCATGATGAAGAATG+GGG 0.675468 2_4:+40846491 Msa0328410:CDS
ATGTTGCTAGAATCAATGGA+AGG 0.678120 2_4:+40845610 Msa0328410:CDS
TCTTTGATCACTGATTCACC+GGG 0.686123 2_4:+40845969 Msa0328410:CDS
TGGCACTTCAACAAGCACAA+AGG 0.702109 2_4:+40846021 Msa0328410:CDS
CTTCAACAAGTATCCCACAC+AGG 0.710515 2_4:-40849195 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTAAAATAATAAGAAG+TGG - chr2_4:40847363-40847382 None:intergenic 10.0%
!! TAAAAAATATTTACCTGAAT+TGG - chr2_4:40846180-40846199 None:intergenic 15.0%
!! TTAAATGTAATTACATGTAT+TGG + chr2_4:40846666-40846685 Msa0328410:intron 15.0%
!!! TTTTTTTTACCTGTAAAATT+TGG - chr2_4:40847222-40847241 None:intergenic 15.0%
!! ATGATTTATATACAAAGTAA+AGG - chr2_4:40847860-40847879 None:intergenic 15.0%
!!! TCTTAAAAAATTGTTTTGAA+TGG + chr2_4:40848139-40848158 Msa0328410:intron 15.0%
!!! AAACTAGTTTTAAATAATCT+TGG - chr2_4:40848957-40848976 None:intergenic 15.0%
!!! CTATTTTCTCAAAATATTAC+TGG - chr2_4:40846641-40846660 None:intergenic 20.0%
!!! TTTGAACTTCTAATTTTAAG+TGG + chr2_4:40846921-40846940 Msa0328410:CDS 20.0%
!!! TACATTAATTTTTTTGCTCT+AGG + chr2_4:40847045-40847064 Msa0328410:intron 20.0%
!!! ACATTAATTTTTTTGCTCTA+GGG + chr2_4:40847046-40847065 Msa0328410:intron 20.0%
!!! AACTTATCTACATAAGTTTT+AGG - chr2_4:40847739-40847758 None:intergenic 20.0%
!! AAGTAAAGGATAAATAAACA+AGG - chr2_4:40847846-40847865 None:intergenic 20.0%
!! ATGTATTTACAATTGATCAA+AGG + chr2_4:40847936-40847955 Msa0328410:intron 20.0%
!! TTCATTTGACAAATACATTT+GGG + chr2_4:40848225-40848244 Msa0328410:CDS 20.0%
!!! AATTTGTATTGATTTGAGAT+TGG - chr2_4:40848268-40848287 None:intergenic 20.0%
!! ATCTCAAATCAATACAAATT+TGG + chr2_4:40848268-40848287 Msa0328410:CDS 20.0%
!! AACAAGAAAAATGTTACTAA+TGG + chr2_4:40848708-40848727 Msa0328410:three_prime_UTR 20.0%
!! GTTATTAAGAGAAATAGATT+TGG + chr2_4:40848732-40848751 Msa0328410:three_prime_UTR 20.0%
!! AGATCAAGATCATTTATTAA+AGG + chr2_4:40848862-40848881 Msa0328410:three_prime_UTR 20.0%
!!! TTGTTAATTTTAAGTTCAAG+CGG - chr2_4:40848934-40848953 None:intergenic 20.0%
!!! AAAGTGTATGAGATTTTTTG+AGG - chr2_4:40845502-40845521 None:intergenic 25.0%
!! GGAGTTTTCATTATGTTTTT+TGG - chr2_4:40845873-40845892 None:intergenic 25.0%
!!! AAGTCAAAGAGTTTTAAAGA+AGG + chr2_4:40845909-40845928 Msa0328410:CDS 25.0%
!!! AGTCAAAGAGTTTTAAAGAA+GGG + chr2_4:40845910-40845929 Msa0328410:CDS 25.0%
! AAAATGAAGATTGCTCATTA+TGG + chr2_4:40846044-40846063 Msa0328410:CDS 25.0%
! TCATGATAAGCAATATAGAT+AGG - chr2_4:40846482-40846501 None:intergenic 25.0%
!! TAGTTTATATGTGTTTCTGA+AGG - chr2_4:40846811-40846830 None:intergenic 25.0%
! TTCAAATAACATCCTGAATA+TGG - chr2_4:40846907-40846926 None:intergenic 25.0%
! ATGTCTATTAGTTCAGAATA+TGG + chr2_4:40847154-40847173 Msa0328410:CDS 25.0%
! ATTGAAATAAGCAAAGTTAG+AGG + chr2_4:40847178-40847197 Msa0328410:CDS 25.0%
!! TAATGCTAACCAAATTTTAC+AGG + chr2_4:40847210-40847229 Msa0328410:CDS 25.0%
! AACAGAAACTTATAAGCTAT+TGG - chr2_4:40847427-40847446 None:intergenic 25.0%
!!! TTGCAGTTAATTTTAACACA+AGG + chr2_4:40847546-40847565 Msa0328410:intron 25.0%
!!! GTTATTTTCATTAACTATCC+TGG + chr2_4:40847692-40847711 Msa0328410:intron 25.0%
! GCTTATTTACAATTGATCAA+AGG + chr2_4:40847958-40847977 Msa0328410:intron 25.0%
! GTTCATTTGACAAATACATT+TGG + chr2_4:40848224-40848243 Msa0328410:CDS 25.0%
! TCATTTGACAAATACATTTG+GGG + chr2_4:40848226-40848245 Msa0328410:CDS 25.0%
! TGTTGTGTATAGTATTAGAT+AGG + chr2_4:40848586-40848605 Msa0328410:three_prime_UTR 25.0%
! AGATCAACTTTAAATGATCA+CGG - chr2_4:40848802-40848821 None:intergenic 25.0%
AGGAGTAATAGTGTTAAGAT+TGG - chr2_4:40845482-40845501 None:intergenic 30.0%
! TTCTTTCAAGATTTGGAACA+TGG - chr2_4:40845659-40845678 None:intergenic 30.0%
GAGTTTCTTCTTTCAAGATT+TGG - chr2_4:40845666-40845685 None:intergenic 30.0%
TTTCCTCTCTTTATAGCTAA+TGG - chr2_4:40845807-40845826 None:intergenic 30.0%
AAGAGAGGAAATGACAATAA+TGG + chr2_4:40845816-40845835 Msa0328410:CDS 30.0%
CATAATGAAAACTCCAACTT+TGG + chr2_4:40845878-40845897 Msa0328410:CDS 30.0%
!!! AGTTTTAAAGAAGGGTCTTT+TGG + chr2_4:40845918-40845937 Msa0328410:CDS 30.0%
AAAGACTACTACTAATCAAG+AGG + chr2_4:40846130-40846149 Msa0328410:CDS 30.0%
!! ACATGTGTGACTTTAGAATT+AGG - chr2_4:40846257-40846276 None:intergenic 30.0%
TTGCTTATCATGATGAAGAA+TGG + chr2_4:40846489-40846508 Msa0328410:CDS 30.0%
TGCTTATCATGATGAAGAAT+GGG + chr2_4:40846490-40846509 Msa0328410:CDS 30.0%
TATGTTACACACAACTATGA+AGG + chr2_4:40846555-40846574 Msa0328410:intron 30.0%
TATTGAGATGTGTCAAACAT+CGG + chr2_4:40846707-40846726 Msa0328410:intron 30.0%
ATGCCTTCAATCTCAAATAT+TGG + chr2_4:40846732-40846751 Msa0328410:intron 30.0%
! ACACCAATATTTGAGATTGA+AGG - chr2_4:40846738-40846757 None:intergenic 30.0%
AGAATTTGATGCAGAAATTG+TGG + chr2_4:40847108-40847127 Msa0328410:CDS 30.0%
CATTTGCTTATCAGTCAAAT+TGG - chr2_4:40847134-40847153 None:intergenic 30.0%
ATTGATCAATCAACTCATGT+GGG - chr2_4:40847456-40847475 None:intergenic 30.0%
!! AATATGTGACAGCATTGATA+AGG - chr2_4:40847492-40847511 None:intergenic 30.0%
ATTCAGCTGTTTATCAAGAT+AGG - chr2_4:40847574-40847593 None:intergenic 30.0%
TATCTTGATAAACAGCTGAA+TGG + chr2_4:40847573-40847592 Msa0328410:intron 30.0%
CACTAATCAACGTATTTAGT+CGG + chr2_4:40847778-40847797 Msa0328410:intron 30.0%
AGGCTTATATACAAAGACAA+AGG + chr2_4:40847978-40847997 Msa0328410:intron 30.0%
!! CTTTGAAATTATGCCATTAC+AGG + chr2_4:40848186-40848205 Msa0328410:intron 30.0%
CATTTGACAAATACATTTGG+GGG + chr2_4:40848227-40848246 Msa0328410:CDS 30.0%
ATAAATGAATGAACCACTGT+TGG - chr2_4:40848373-40848392 None:intergenic 30.0%
TGATTAAGTGGTCATTTGTT+AGG - chr2_4:40848408-40848427 None:intergenic 30.0%
GGGTCAATTAATCTCTTATA+TGG - chr2_4:40848467-40848486 None:intergenic 30.0%
TTATGTCTTGAGAACTTGTA+GGG - chr2_4:40848487-40848506 None:intergenic 30.0%
TTTATGTCTTGAGAACTTGT+AGG - chr2_4:40848488-40848507 None:intergenic 30.0%
! TATGCTGACTATAGTTTACT+TGG + chr2_4:40848556-40848575 Msa0328410:three_prime_UTR 30.0%
AAATAATGCAAAGTCCTTGT+AGG - chr2_4:40849160-40849179 None:intergenic 30.0%
AAATGAGGGAAATGTTGTGA+GGG - chr2_4:40845442-40845461 None:intergenic 35.0%
AAAATGAGGGAAATGTTGTG+AGG - chr2_4:40845443-40845462 None:intergenic 35.0%
GAAAAGAGAGAGGAAAATGA+GGG - chr2_4:40845456-40845475 None:intergenic 35.0%
TGAAAAGAGAGAGGAAAATG+AGG - chr2_4:40845457-40845476 None:intergenic 35.0%
AGATTGGAATGAAAAGAGAG+AGG - chr2_4:40845466-40845485 None:intergenic 35.0%
TACAGTTACTTCTTTAGCCT+TGG - chr2_4:40845565-40845584 None:intergenic 35.0%
AAGGCTAAAGAAGTAACTGT+AGG + chr2_4:40845564-40845583 Msa0328410:CDS 35.0%
ATGTTGCTAGAATCAATGGA+AGG + chr2_4:40845610-40845629 Msa0328410:CDS 35.0%
!!! GGTAATGGAGAAAGTGATTT+TGG - chr2_4:40845705-40845724 None:intergenic 35.0%
!! GAAGTAGTAGTAGTCTTGTT+TGG - chr2_4:40845732-40845751 None:intergenic 35.0%
!!! TTTGGTTTTGGAGAAATTGG+TGG - chr2_4:40845765-40845784 None:intergenic 35.0%
!!! GACTTTGGTTTTGGAGAAAT+TGG - chr2_4:40845768-40845787 None:intergenic 35.0%
AGACCATTAGCTATAAAGAG+AGG + chr2_4:40845801-40845820 Msa0328410:CDS 35.0%
!! TTCTTTGATCACTGATTCAC+CGG + chr2_4:40845968-40845987 Msa0328410:CDS 35.0%
!! TTTGAATCAAGAGCACTTGA+AGG - chr2_4:40846113-40846132 None:intergenic 35.0%
ATTGCACTCGGTATCAAAAA+AGG - chr2_4:40846404-40846423 None:intergenic 35.0%
AGCTAAAGCAAAATTGCACT+CGG - chr2_4:40846416-40846435 None:intergenic 35.0%
GCTTATCATGATGAAGAATG+GGG + chr2_4:40846491-40846510 Msa0328410:CDS 35.0%
GTAACATAGAGAGAGAAGTA+GGG - chr2_4:40846543-40846562 None:intergenic 35.0%
TGTAACATAGAGAGAGAAGT+AGG - chr2_4:40846544-40846563 None:intergenic 35.0%
!! TATTGGTGTCATCTTTGTGT+TGG + chr2_4:40846683-40846702 Msa0328410:intron 35.0%
ATGATGTTGTGTCCATATTC+AGG + chr2_4:40846892-40846911 Msa0328410:intron 35.0%
!! ATTTTAAGTGGTGCTCAAGT+TGG + chr2_4:40846933-40846952 Msa0328410:CDS 35.0%
AATTCACTCGTTGCCAAAAA+AGG + chr2_4:40847384-40847403 Msa0328410:intron 35.0%
TTGATCAATCAACTCATGTG+GGG - chr2_4:40847455-40847474 None:intergenic 35.0%
CATTGATCAATCAACTCATG+TGG - chr2_4:40847457-40847476 None:intergenic 35.0%
! CTCTTATTTACATGAGTTCC+AGG - chr2_4:40847713-40847732 None:intergenic 35.0%
!! TAGTGAGTGTTGATAGCTTA+TGG - chr2_4:40847763-40847782 None:intergenic 35.0%
!! AATTAGAGCATTGAGAAGTC+AGG - chr2_4:40848034-40848053 None:intergenic 35.0%
ATGCCATTACAGGTTAAGAA+AGG + chr2_4:40848196-40848215 Msa0328410:intron 35.0%
AAGCCTTTCTTAACCTGTAA+TGG - chr2_4:40848202-40848221 None:intergenic 35.0%
TTACAGGTTAAGAAAGGCTT+TGG + chr2_4:40848202-40848221 Msa0328410:intron 35.0%
!! TCTGATTTTGATGTCTTCAC+TGG - chr2_4:40848304-40848323 None:intergenic 35.0%
AGCAAAGACATGATTAAGAG+AGG + chr2_4:40848331-40848350 Msa0328410:CDS 35.0%
GCAAAGACATGATTAAGAGA+GGG + chr2_4:40848332-40848351 Msa0328410:CDS 35.0%
AAGAGAGGGTTTAGATATGT+TGG + chr2_4:40848346-40848365 Msa0328410:CDS 35.0%
AGTGGTTCATTCATTTATGC+AGG + chr2_4:40848375-40848394 Msa0328410:CDS 35.0%
AAATGATCTTGATCTTGAGC+TGG - chr2_4:40848857-40848876 None:intergenic 35.0%
CGATCTGACATCAGATTTAA+CGG + chr2_4:40848980-40848999 Msa0328410:three_prime_UTR 35.0%
! ATACTTGTTGAAGAGTGTGT+AGG + chr2_4:40849205-40849224 Msa0328410:three_prime_UTR 35.0%
! TACTTGTTGAAGAGTGTGTA+GGG + chr2_4:40849206-40849225 Msa0328410:three_prime_UTR 35.0%
AAATGTTGTGAGGGATTGGA+GGG - chr2_4:40845433-40845452 None:intergenic 40.0%
ACAAAACATGTGTAGCTCCA+AGG + chr2_4:40845545-40845564 Msa0328410:exon 40.0%
!! CCATTGATTCTAGCAACATG+TGG - chr2_4:40845609-40845628 None:intergenic 40.0%
CCACATGTTGCTAGAATCAA+TGG + chr2_4:40845606-40845625 Msa0328410:CDS 40.0%
! TTGCATGTTGGTTGAAGTAC+TGG - chr2_4:40845636-40845655 None:intergenic 40.0%
! TTTGGAACATGGTTGCATGT+TGG - chr2_4:40845648-40845667 None:intergenic 40.0%
!! GTCTTGTTTGGAAGTGGTAA+TGG - chr2_4:40845720-40845739 None:intergenic 40.0%
!! GTAGTAGTCTTGTTTGGAAG+TGG - chr2_4:40845726-40845745 None:intergenic 40.0%
!! CTAGTAGGTGACTTTGGTTT+TGG - chr2_4:40845777-40845796 None:intergenic 40.0%
CTAGTACTAGTAGGTGACTT+TGG - chr2_4:40845783-40845802 None:intergenic 40.0%
GCTAATGGTCTAGTACTAGT+AGG - chr2_4:40845792-40845811 None:intergenic 40.0%
! TGAAAACTCCAACTTTGGAG+AGG + chr2_4:40845883-40845902 Msa0328410:CDS 40.0%
!! GACTTTTTCCTCTCCAAAGT+TGG - chr2_4:40845894-40845913 None:intergenic 40.0%
!! TCTTTGATCACTGATTCACC+GGG + chr2_4:40845969-40845988 Msa0328410:CDS 40.0%
AAGCATAGCTGCTGTTAGAA+GGG + chr2_4:40845992-40846011 Msa0328410:CDS 40.0%
! AGAGCACTTGAAGGATCAAT+AGG - chr2_4:40846104-40846123 None:intergenic 40.0%
GCTTACTTGTCATCATGAAC+TGG - chr2_4:40846521-40846540 None:intergenic 40.0%
!! ATTGGTGTCGAATACTGACA+CGG + chr2_4:40846750-40846769 Msa0328410:intron 40.0%
CAGAATCAAACCTGAAGTCT+AGG - chr2_4:40846989-40847008 None:intergenic 40.0%
!!! AACTTGTCTGCAGCCTTTTT+TGG - chr2_4:40847400-40847419 None:intergenic 40.0%
GCATTGAGAAGTCAGGATAA+TGG - chr2_4:40848027-40848046 None:intergenic 40.0%
AAACACCTGCGTTATTCGTA+AGG - chr2_4:40848094-40848113 None:intergenic 40.0%
TAGATATGTTGGTCCAACAG+TGG + chr2_4:40848357-40848376 Msa0328410:CDS 40.0%
GCACTTGCAATGCACATTAT+TGG + chr2_4:40848438-40848457 Msa0328410:CDS 40.0%
!!! TAGAAGCGTGCTTTTTCAAC+AGG + chr2_4:40849023-40849042 Msa0328410:three_prime_UTR 40.0%
!!! AGAAGCGTGCTTTTTCAACA+GGG + chr2_4:40849024-40849043 Msa0328410:three_prime_UTR 40.0%
GAAATGTTGTGAGGGATTGG+AGG - chr2_4:40845434-40845453 None:intergenic 45.0%
AGGGAAATGTTGTGAGGGAT+TGG - chr2_4:40845437-40845456 None:intergenic 45.0%
! TCTAGCAACATGTGGTGTTG+TGG - chr2_4:40845601-40845620 None:intergenic 45.0%
GAAGCATAGCTGCTGTTAGA+AGG + chr2_4:40845991-40846010 Msa0328410:CDS 45.0%
TGCTGTTAGAAGGGAACAAG+TGG + chr2_4:40846001-40846020 Msa0328410:CDS 45.0%
TGGCACTTCAACAAGCACAA+AGG + chr2_4:40846021-40846040 Msa0328410:CDS 45.0%
AGCTTCATCACAGCCAATTC+AGG + chr2_4:40846164-40846183 Msa0328410:CDS 45.0%
AGGACACAACTATGAAGCAC+CGG + chr2_4:40846575-40846594 Msa0328410:intron 45.0%
TCTGATGTATGTGCATGTGC+CGG - chr2_4:40846597-40846616 None:intergenic 45.0%
!! GTGCTCAAGTTGGATCTGAT+TGG + chr2_4:40846943-40846962 Msa0328410:CDS 45.0%
GAAGTCTAGGCGTTTCTTCA+AGG - chr2_4:40846976-40846995 None:intergenic 45.0%
TGAAGAAACGCCTAGACTTC+AGG + chr2_4:40846976-40846995 Msa0328410:CDS 45.0%
!! CGGTTAAAGCATCGATGAGT+CGG + chr2_4:40847798-40847817 Msa0328410:intron 45.0%
!! TAGCCAGTGAGTGTTGATAC+AGG + chr2_4:40848064-40848083 Msa0328410:intron 45.0%
TAGCCTGTATCAACACTCAC+TGG - chr2_4:40848070-40848089 None:intergenic 45.0%
GCTATCCTTACGAATAACGC+AGG + chr2_4:40848086-40848105 Msa0328410:intron 45.0%
CATTCATTTATGCAGGCAGC+TGG + chr2_4:40848382-40848401 Msa0328410:CDS 45.0%
ACCACTTAATCACTTGCCAC+AGG + chr2_4:40848416-40848435 Msa0328410:CDS 45.0%
!!! GAAGCGTGCTTTTTCAACAG+GGG + chr2_4:40849025-40849044 Msa0328410:three_prime_UTR 45.0%
GGAATCCGTTAAGAAGTGGT+GGG + chr2_4:40849046-40849065 Msa0328410:three_prime_UTR 45.0%
ACACTCCCACCACTTCTTAA+CGG - chr2_4:40849054-40849073 None:intergenic 45.0%
!! TAAGAAGTGGTGGGAGTGTT+AGG + chr2_4:40849055-40849074 Msa0328410:three_prime_UTR 45.0%
!! AAGAAGTGGTGGGAGTGTTA+GGG + chr2_4:40849056-40849075 Msa0328410:three_prime_UTR 45.0%
CAGCATGTGCATGTGCAAAT+AGG + chr2_4:40849085-40849104 Msa0328410:three_prime_UTR 45.0%
CATGTGCATGTGCAAATAGG+AGG + chr2_4:40849088-40849107 Msa0328410:three_prime_UTR 45.0%
CATGTGCAAATAGGAGGCAA+GGG + chr2_4:40849094-40849113 Msa0328410:three_prime_UTR 45.0%
CAAAGTCCTTGTAGGTCACA+TGG - chr2_4:40849152-40849171 None:intergenic 45.0%
TTCCCTTTAACAGCCTGTGT+GGG + chr2_4:40849182-40849201 Msa0328410:three_prime_UTR 45.0%
ATCCCACACAGGCTGTTAAA+GGG - chr2_4:40849187-40849206 None:intergenic 45.0%
TATCCCACACAGGCTGTTAA+AGG - chr2_4:40849188-40849207 None:intergenic 45.0%
CTTCAACAAGTATCCCACAC+AGG - chr2_4:40849198-40849217 None:intergenic 45.0%
CTAACAGCAGCTATGCTTCC+CGG - chr2_4:40845990-40846009 None:intergenic 50.0%
TAGGCGTTTCTTCAAGGTGG+AGG - chr2_4:40846970-40846989 None:intergenic 50.0%
GTCTAGGCGTTTCTTCAAGG+TGG - chr2_4:40846973-40846992 None:intergenic 50.0%
GCCTGTGGCAAGTGATTAAG+TGG - chr2_4:40848420-40848439 None:intergenic 50.0%
ATGTGCATTGCAAGTGCCTG+TGG - chr2_4:40848435-40848454 None:intergenic 50.0%
TAACTGCGTCAAGCTCAAGC+AGG + chr2_4:40848760-40848779 Msa0328410:three_prime_UTR 50.0%
CAGGGGAATCCGTTAAGAAG+TGG + chr2_4:40849042-40849061 Msa0328410:three_prime_UTR 50.0%
GGGAATCCGTTAAGAAGTGG+TGG + chr2_4:40849045-40849064 Msa0328410:three_prime_UTR 50.0%
GCATGTGCAAATAGGAGGCA+AGG + chr2_4:40849093-40849112 Msa0328410:three_prime_UTR 50.0%
GAGAGACCATGTGACCTACA+AGG + chr2_4:40849143-40849162 Msa0328410:three_prime_UTR 50.0%
GTTCCCTTTAACAGCCTGTG+TGG + chr2_4:40849181-40849200 Msa0328410:three_prime_UTR 50.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 40845430 40849248 40845430 ID=Msa0328410;Name=Msa0328410
chr2_4 mRNA 40845430 40849248 40845430 ID=Msa0328410-mRNA-1;Parent=Msa0328410;Name=Msa0328410-mRNA-1;_AED=0.02;_eAED=0.02;_QI=122|1|1|1|1|1|5|777|391
chr2_4 exon 40845430 40846185 40845430 ID=Msa0328410-mRNA-1:exon:12458;Parent=Msa0328410-mRNA-1
chr2_4 exon 40846477 40846534 40846477 ID=Msa0328410-mRNA-1:exon:12459;Parent=Msa0328410-mRNA-1
chr2_4 exon 40846914 40846997 40846914 ID=Msa0328410-mRNA-1:exon:12460;Parent=Msa0328410-mRNA-1
chr2_4 exon 40847096 40847231 40847096 ID=Msa0328410-mRNA-1:exon:12461;Parent=Msa0328410-mRNA-1
chr2_4 exon 40848208 40849248 40848208 ID=Msa0328410-mRNA-1:exon:12462;Parent=Msa0328410-mRNA-1
chr2_4 five_prime_UTR 40845430 40845551 40845430 ID=Msa0328410-mRNA-1:five_prime_utr;Parent=Msa0328410-mRNA-1
chr2_4 CDS 40845552 40846185 40845552 ID=Msa0328410-mRNA-1:cds;Parent=Msa0328410-mRNA-1
chr2_4 CDS 40846477 40846534 40846477 ID=Msa0328410-mRNA-1:cds;Parent=Msa0328410-mRNA-1
chr2_4 CDS 40846914 40846997 40846914 ID=Msa0328410-mRNA-1:cds;Parent=Msa0328410-mRNA-1
chr2_4 CDS 40847096 40847231 40847096 ID=Msa0328410-mRNA-1:cds;Parent=Msa0328410-mRNA-1
chr2_4 CDS 40848208 40848471 40848208 ID=Msa0328410-mRNA-1:cds;Parent=Msa0328410-mRNA-1
chr2_4 three_prime_UTR 40848472 40849248 40848472 ID=Msa0328410-mRNA-1:three_prime_utr;Parent=Msa0328410-mRNA-1
Gene Sequence

>Msa0328410

ATGTGTAGCTCCAAGGCTAAAGAAGTAACTGTAGGCATAGAATCTTCCACAACACCACATGTTGCTAGAATCAATGGAAGGCCAGTACTTCAACCAACATGCAACCATGTTCCAAATCTTGAAAGAAGAAACTCAATTAAGAAATCAACACCAAAATCACTTTCTCCATTACCACTTCCAAACAAGACTACTACTACTTCTTCATTGACACCACCAATTTCTCCAAAACCAAAGTCACCTACTAGTACTAGACCATTAGCTATAAAGAGAGGAAATGACAATAATGGACTTAATTTAAGCTGTGAAAAGATTTCAATACCAAAAAACATAATGAAAACTCCAACTTTGGAGAGGAAAAAGTCAAAGAGTTTTAAAGAAGGGTCTTTTGGTATTGAAGCTTCGTTGAGTTATTCTTCTTCTTTGATCACTGATTCACCGGGAAGCATAGCTGCTGTTAGAAGGGAACAAGTGGCACTTCAACAAGCACAAAGGAAAATGAAGATTGCTCATTATGGAAGATCAAAGTCTGCTAAATTTGAAAGAGTTTTTCCTATTGATCCTTCAAGTGCTCTTGATTCAAAGACTACTACTAATCAAGAGGAGAAGAGATGCAGCTTCATCACAGCCAATTCAGATCCTATCTATATTGCTTATCATGATGAAGAATGGGGAGTTCCAGTTCATGATGACAAGATGTTATTTGAACTTCTAATTTTAAGTGGTGCTCAAGTTGGATCTGATTGGACCTCCACCTTGAAGAAACGCCTAGACTTCAGAGCTGCATTTTCAGAATTTGATGCAGAAATTGTGGCCAATTTGACTGATAAGCAAATGATGTCTATTAGTTCAGAATATGGCATTGAAATAAGCAAAGTTAGAGGAGTTGTTGATAATGCTAACCAAATTTTACAGGTTAAGAAAGGCTTTGGTTCATTTGACAAATACATTTGGGGGTTTGTGAATCATAAACCAATCTCAAATCAATACAAATTTGGTCACAAGATTCCAGTGAAGACATCAAAATCAGAGAGCATAAGCAAAGACATGATTAAGAGAGGGTTTAGATATGTTGGTCCAACAGTGGTTCATTCATTTATGCAGGCAGCTGGCCTAACAAATGACCACTTAATCACTTGCCACAGGCACTTGCAATGCACATTATTGGCAGCCATATAA
Protein sequence

>Msa0328410

MCSSKAKEVTVGIESSTTPHVARINGRPVLQPTCNHVPNLERRNSIKKSTPKSLSPLPLPNKTTTTSSLTPPISPKPKSPTSTRPLAIKRGNDNNGLNLSCEKISIPKNIMKTPTLERKKSKSFKEGSFGIEASLSYSSSLITDSPGSIAAVRREQVALQQAQRKMKIAHYGRSKSAKFERVFPIDPSSALDSKTTTNQEEKRCSFITANSDPIYIAYHDEEWGVPVHDDKMLFELLILSGAQVGSDWTSTLKKRLDFRAAFSEFDAEIVANLTDKQMMSISSEYGIEISKVRGVVDNANQILQVKKGFGSFDKYIWGFVNHKPISNQYKFGHKIPVKTSKSESISKDMIKRGFRYVGPTVVHSFMQAAGLTNDHLITCHRHLQCTLLAAI*