AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0555090

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0555090 MtrunA17_Chr4g0040891 98.489 331 1 2 1 330 1 328 0.0 664
Msa0555090 MtrunA17_Chr2g0325131 62.974 343 92 12 1 329 1 322 2.65e-120 350
Msa0555090 MtrunA17_Chr2g0316551 51.420 352 117 16 1 315 1 335 8.14e-90 272
Msa0555090 MtrunA17_Chr8g0380611 52.941 102 43 1 191 292 228 324 1.21e-23 100
Msa0555090 MtrunA17_Chr5g0432701 48.387 93 48 0 197 289 159 251 4.48e-20 89.7
Msa0555090 MtrunA17_Chr4g0036071 37.589 141 65 2 174 291 314 454 5.04e-20 90.5
Msa0555090 MtrunA17_Chr2g0329551 46.000 100 54 0 195 294 219 318 2.16e-19 88.6
Msa0555090 MtrunA17_Chr6g0463571 33.523 176 103 2 136 311 96 257 1.12e-18 85.1
Msa0555090 MtrunA17_Chr3g0107491 45.161 93 51 0 197 289 177 269 2.75e-18 84.7
Msa0555090 MtrunA17_Chr3g0128781 44.681 94 52 0 196 289 107 200 3.46e-17 80.9
Msa0555090 MtrunA17_Chr1g0156051 48.980 98 46 2 196 291 386 481 4.49e-13 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0555090 AT3G58120.1 54.775 356 108 13 1 330 1 329 9.99e-105 310
Msa0555090 AT2G42380.2 53.529 340 128 9 1 330 1 320 9.56e-101 300
Msa0555090 AT2G42380.1 50.000 340 129 10 1 330 1 309 2.70e-89 270
Msa0555090 AT3G58120.2 56.122 294 98 10 1 276 1 281 3.59e-87 263
Msa0555090 AT2G42380.5 53.873 284 104 7 1 276 1 265 6.65e-83 252
Msa0555090 AT2G42380.3 46.939 245 103 7 1 237 1 226 5.48e-53 174
Msa0555090 AT2G42380.4 46.939 245 103 7 1 237 1 226 5.48e-53 174
Msa0555090 AT1G58110.1 29.552 335 162 10 2 292 20 324 8.24e-29 114
Msa0555090 AT1G58110.2 29.552 335 162 10 2 292 20 324 8.24e-29 114
Msa0555090 AT1G43700.1 49.485 97 49 0 195 291 192 288 1.40e-20 91.3
Msa0555090 AT4G38900.2 47.368 95 50 0 195 289 392 486 1.01e-19 90.1
Msa0555090 AT4G38900.3 47.368 95 50 0 195 289 392 486 1.01e-19 90.1
Msa0555090 AT2G40620.1 41.739 115 56 1 195 309 146 249 4.53e-19 87.4
Msa0555090 AT1G06070.1 44.000 100 56 0 195 294 208 307 1.16e-18 86.7
Msa0555090 AT2G21230.4 45.361 97 53 0 195 291 368 464 2.17e-18 86.3
Msa0555090 AT2G21230.1 45.361 97 53 0 195 291 368 464 2.17e-18 86.3
Msa0555090 AT1G06850.1 47.312 93 49 0 197 289 148 240 2.34e-18 85.1
Msa0555090 AT2G31370.5 42.991 107 61 0 188 294 192 298 4.47e-18 84.7
Msa0555090 AT2G31370.2 28.287 251 152 6 63 294 57 298 5.25e-18 84.7
Msa0555090 AT2G31370.4 28.287 251 152 6 63 294 57 298 5.25e-18 84.7
Msa0555090 AT2G31370.1 28.287 251 152 6 63 294 57 298 5.25e-18 84.7
Msa0555090 AT2G31370.7 28.287 251 152 6 63 294 57 298 5.25e-18 84.7
Msa0555090 AT2G31370.3 28.287 251 152 6 63 294 57 298 5.25e-18 84.7
Msa0555090 AT2G31370.6 28.287 251 152 6 63 294 57 298 5.25e-18 84.7
Msa0555090 AT4G38900.1 44.554 101 50 1 195 289 392 492 1.37e-17 84.0
Msa0555090 AT5G04840.1 50.000 84 42 0 206 289 177 260 3.45e-17 81.3
Msa0555090 AT1G35490.2 41.509 106 56 2 185 289 158 258 8.15e-17 80.1
Msa0555090 AT1G35490.1 41.509 106 56 2 185 289 158 258 8.15e-17 80.1
Msa0555090 AT2G21230.3 41.748 103 54 1 195 291 368 470 5.46e-16 79.0
Msa0555090 AT2G21230.2 45.122 82 45 0 195 276 368 449 2.04e-14 74.3
Msa0555090 AT5G04840.2 51.389 72 35 0 206 277 177 248 8.64e-14 70.5
Msa0555090 AT5G07160.1 41.489 94 55 0 196 289 47 140 4.56e-12 63.5
Msa0555090 AT1G06850.2 68.889 45 14 0 196 240 147 191 5.36e-11 62.4
Msa0555090 AT2G12900.1 34.021 97 64 0 195 291 114 210 7.47e-11 62.4

Find 104 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTTATTTGACGTATTAAT+TGG 0.161175 4_1:+52817302 Msa0555090:three_prime_UTR
TATTTCTTACTACTAAAATT+TGG 0.212403 4_1:+52816711 Msa0555090:three_prime_UTR
GTCACCACGGGTTGCATATT+TGG 0.213119 4_1:+52815691 Msa0555090:CDS
TGATGTGTTTACTTTATATA+AGG 0.232996 4_1:+52816898 Msa0555090:three_prime_UTR
TGTCTGCTGCTTTAACTATA+TGG 0.238898 4_1:+52817024 Msa0555090:three_prime_UTR
ACGTCTGTGTGCCCCTCTTC+TGG 0.291136 4_1:-52814652 None:intergenic
TCATTATCACATCGTGGTTT+AGG 0.296604 4_1:-52816562 None:intergenic
GTCTGCTGCTTTAACTATAT+GGG 0.299516 4_1:+52817025 Msa0555090:three_prime_UTR
GGTGAATGCAAACAGGAAAA+TGG 0.353861 4_1:+52814975 Msa0555090:CDS
GGCCAAGTTGGTGTCATGTT+TGG 0.363718 4_1:-52814507 None:intergenic
ATCACACATTTCATTTGTTA+AGG 0.364727 4_1:+52814391 Msa0555090:five_prime_UTR
GAATCTGATGAGGATGAAAT+TGG 0.366529 4_1:+52814954 Msa0555090:CDS
ACCACTCAAAGTTGCATGAT+TGG 0.375136 4_1:+52816923 Msa0555090:three_prime_UTR
ACGAAGTGTGACTTCATTAC+AGG 0.377047 4_1:+52815501 Msa0555090:CDS
ACAATCAACAAAACCCTTCA+TGG 0.391610 4_1:+52814601 Msa0555090:CDS
GATGTGTTTACTTTATATAA+GGG 0.394891 4_1:+52816899 Msa0555090:three_prime_UTR
GTTGGTGTCATGTTTGGAAT+TGG 0.398684 4_1:-52814501 None:intergenic
ATGTTTGGAATTGGAACCTT+TGG 0.398984 4_1:-52814492 None:intergenic
AATGATGAAGATGACAATAA+TGG 0.401593 4_1:+52815008 Msa0555090:CDS
CAGTGAAATCTTGCCTCGTA+TGG 0.424007 4_1:+52817218 Msa0555090:three_prime_UTR
TGCCTTTCTAACATTCCCTC+TGG 0.445979 4_1:+52814213 None:intergenic
GAAAAGTTTGAAGCATTACT+TGG 0.449386 4_1:-52814576 None:intergenic
TTAGGAAATTGAATTGATGA+AGG 0.452878 4_1:+52816817 Msa0555090:three_prime_UTR
TAAGGCAATTCCACTGTAGC+TGG 0.460035 4_1:+52814409 Msa0555090:five_prime_UTR
TGAAATTGGTGAATGCAAAC+AGG 0.473536 4_1:+52814968 Msa0555090:CDS
AATAATTAAGATGATTAAGA+AGG 0.473819 4_1:+52816745 Msa0555090:three_prime_UTR
ATTATTGATGCTGCTTTCCA+TGG 0.478771 4_1:+52817268 Msa0555090:three_prime_UTR
AAGAGGGGCACACAGACGTT+CGG 0.489835 4_1:+52814656 Msa0555090:CDS
GTAGCTGATGGTGCTGGTGG+TGG 0.496771 4_1:-52814825 None:intergenic
ATGGAGACAATGAGTTTGAA+AGG 0.498812 4_1:+52814736 Msa0555090:CDS
TGAAGCATTACTTGGTGAGA+TGG 0.500952 4_1:-52814568 None:intergenic
ACTACTAAAATTTGGAAGGA+AGG 0.502414 4_1:+52816719 Msa0555090:three_prime_UTR
TCACATCGTGGTTTAGGTGA+TGG 0.507950 4_1:-52816556 None:intergenic
TTATGATCAGAAGGAGTTGA+AGG 0.508247 4_1:-52814867 None:intergenic
TAAAGATGATTACATATCCA+TGG 0.514742 4_1:-52817285 None:intergenic
TGTTGGGATGGATGAAAGGA+AGG 0.518715 4_1:+52817186 Msa0555090:three_prime_UTR
TTCCAAACATGACACCAACT+TGG 0.521076 4_1:+52814505 Msa0555090:CDS
CTGACCCCAAGAGGGTCAAA+AGG 0.522349 4_1:+52815063 Msa0555090:CDS
ATAATTAAGATGATTAAGAA+GGG 0.523437 4_1:+52816746 Msa0555090:three_prime_UTR
AGTGAAATCTTGCCTCGTAT+GGG 0.524180 4_1:+52817219 Msa0555090:three_prime_UTR
TCTGCTGCTTTAACTATATG+GGG 0.525495 4_1:+52817026 Msa0555090:three_prime_UTR
GAGAAGAAATAAACAGTACT+TGG 0.525538 4_1:+52816992 Msa0555090:three_prime_UTR
TGATGGAGCATTGTCGTCGT+AGG 0.526258 4_1:+52814712 Msa0555090:CDS
TTTGGAATTGGAACCTTTGG+TGG 0.526554 4_1:-52814489 None:intergenic
GGGTTTATGTAATGTAATGT+TGG 0.526786 4_1:+52817046 Msa0555090:three_prime_UTR
TATGAAGGTCACTGAGTCAC+TGG 0.527916 4_1:-52814679 None:intergenic
AGAAACTCGTCCACCCATGA+AGG 0.530492 4_1:-52814615 None:intergenic
CAAGACAAAATCTTCAAAGA+TGG 0.531158 4_1:+52815779 Msa0555090:CDS
CATCATCGATGCTTCTATGA+AGG 0.532117 4_1:-52814694 None:intergenic
TGGTGCTGGTGGTGGCATCA+TGG 0.538097 4_1:-52814817 None:intergenic
CTTCATAGAAGCATCGATGA+TGG 0.538192 4_1:+52814695 Msa0555090:CDS
TCCAATCATGCAACTTTGAG+TGG 0.539330 4_1:-52816924 None:intergenic
TAAACAGTACTTGGTGGTGT+TGG 0.549994 4_1:+52817001 Msa0555090:three_prime_UTR
ATGTGTTTACTTTATATAAG+GGG 0.553319 4_1:+52816900 Msa0555090:three_prime_UTR
AGAGGGAGATAGAGAGGCTG+AGG 0.553494 4_1:+52816473 Msa0555090:CDS
ATTTCTCGTCCACCAGAAGA+GGG 0.554513 4_1:+52814640 Msa0555090:CDS
GAGCATTGTCGTCGTAGGGA+TGG 0.556038 4_1:+52814717 Msa0555090:CDS
TCTTACTACTAAAATTTGGA+AGG 0.559578 4_1:+52816715 Msa0555090:three_prime_UTR
AGTATCAGTGCTGTCACCAC+GGG 0.561461 4_1:+52815679 Msa0555090:CDS
CAATCAACAAAACCCTTCAT+GGG 0.563412 4_1:+52814602 Msa0555090:CDS
GATTTCTCGTCCACCAGAAG+AGG 0.564070 4_1:+52814639 Msa0555090:CDS
TGATGACAAAGAGGAAGATG+AGG 0.565693 4_1:+52814899 Msa0555090:CDS
AATGAAGAAGCATGTAGCAT+AGG 0.566269 4_1:-52814270 None:intergenic
TGACAAAGAGGAAGATGAGG+AGG 0.566303 4_1:+52814902 Msa0555090:CDS
AAGAAATAAACAGTACTTGG+TGG 0.568573 4_1:+52816995 Msa0555090:three_prime_UTR
GAAGAAAATTCAGGCCAAGT+TGG 0.570645 4_1:-52814519 None:intergenic
TTAGTGTAGACGAGGTTTCG+AGG 0.573188 4_1:+52814787 Msa0555090:CDS
AATATCAAGAATGACAACAT+AGG 0.574028 4_1:-52816645 None:intergenic
ACGTGATGGGAGAATATAAG+TGG 0.580132 4_1:+52816843 Msa0555090:three_prime_UTR
CATTGAAGAGGGAGATAGAG+AGG 0.587016 4_1:+52816467 Msa0555090:CDS
TTGATGCTGTTATGATCAGA+AGG 0.588109 4_1:-52814876 None:intergenic
AACAAACTAGCCAGCTACAG+TGG 0.588237 4_1:-52814419 None:intergenic
TTGCATATTTGGATCATCAG+AGG 0.590830 4_1:+52815702 Msa0555090:CDS
AGTTGGAAAACAAAGACTAG+TGG 0.592807 4_1:+52817158 Msa0555090:three_prime_UTR
TATGATCAGAAGGAGTTGAA+GGG 0.593074 4_1:-52814866 None:intergenic
CTCATCAAGAAGCATTGAAG+AGG 0.593087 4_1:+52816455 Msa0555090:CDS
TCTGTGTGCCCCTCTTCTGG+TGG 0.594474 4_1:-52814649 None:intergenic
AAAGACATGTATGTATGTGA+AGG 0.595881 4_1:-52814238 None:intergenic
GACAATGTAGCTGATGGTGC+TGG 0.596193 4_1:-52814831 None:intergenic
GTCCATGTTTAGTGTAGACG+AGG 0.601046 4_1:+52814779 Msa0555090:CDS
CAGCATCAATGATGACAAAG+AGG 0.602367 4_1:+52814890 Msa0555090:CDS
GATGGAGCATTGTCGTCGTA+GGG 0.605532 4_1:+52814713 Msa0555090:CDS
TCAACAAAACCCTTCATGGG+TGG 0.609845 4_1:+52814605 Msa0555090:CDS
TCTTATCAATGTTTGAGACA+TGG 0.612460 4_1:+52816606 Msa0555090:exon
ATTGATGAAGGATAACGTGA+TGG 0.621428 4_1:+52816829 Msa0555090:three_prime_UTR
AACCTCGTCTACACTAAACA+TGG 0.622608 4_1:-52814781 None:intergenic
AATGTAGCTGATGGTGCTGG+TGG 0.623330 4_1:-52814828 None:intergenic
AAGTATCAGTGCTGTCACCA+CGG 0.625333 4_1:+52815678 Msa0555090:CDS
TATGGCTCAATTGCCACCAA+AGG 0.629635 4_1:+52814476 Msa0555090:exon
TCATCAAGAAGCATTGAAGA+GGG 0.631177 4_1:+52816456 Msa0555090:CDS
GAAACTCGTCCACCCATGAA+GGG 0.631276 4_1:-52814614 None:intergenic
CTGCACAAAGATCAAGAGTG+AGG 0.635769 4_1:+52815452 Msa0555090:CDS
TTGATGAAGGATAACGTGAT+GGG 0.648021 4_1:+52816830 Msa0555090:three_prime_UTR
TTGATATTGATGTCACTGAG+TGG 0.663712 4_1:-52816968 None:intergenic
TTTCTCGTCCACCAGAAGAG+GGG 0.668213 4_1:+52814641 Msa0555090:CDS
ATTGAAGCATGAATCTGATG+AGG 0.673573 4_1:+52814944 Msa0555090:CDS
ATGTATGTGAAGGAGCCAGA+GGG 0.675920 4_1:-52814228 None:intergenic
TATGTATGTGAAGGAGCCAG+AGG 0.683769 4_1:-52814229 None:intergenic
GAATGCAAACAGGAAAATGG+AGG 0.685882 4_1:+52814978 Msa0555090:CDS
CGTGGTTTAGGTGATGGAGA+TGG 0.696148 4_1:-52816550 None:intergenic
TGAGTATCATTATCACATCG+TGG 0.696934 4_1:-52816568 None:intergenic
AACAAATCAAAACCCATACG+AGG 0.699316 4_1:-52817231 None:intergenic
TTAGATGACAATGTAGCTGA+TGG 0.701045 4_1:-52814837 None:intergenic
TGATCCAAATATGCAACCCG+TGG 0.752305 4_1:-52815695 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATAATTAAACAATTTTG+AGG - chr4_1:52816053-52816072 None:intergenic 10.0%
!!! TGTTTAATTATTTTTACTAT+AGG + chr4_1:52816059-52816078 Msa0555090:intron 10.0%
!! GAATGAAAAAACTATATAAT+AGG + chr4_1:52816108-52816127 Msa0555090:intron 15.0%
!!! ATCTATAAGTATTATTTTGA+AGG - chr4_1:52816390-52816409 None:intergenic 15.0%
!!! CTTGATATTTTATTTTTAGA+AGG + chr4_1:52816659-52816678 Msa0555090:three_prime_UTR 15.0%
!!! AATTTTAGTAGTAAGAAATA+GGG - chr4_1:52816711-52816730 None:intergenic 15.0%
!!! AAATTTTAGTAGTAAGAAAT+AGG - chr4_1:52816712-52816731 None:intergenic 15.0%
!! TATTTCTTACTACTAAAATT+TGG + chr4_1:52816711-52816730 Msa0555090:three_prime_UTR 15.0%
!! AATAATTAAGATGATTAAGA+AGG + chr4_1:52816745-52816764 Msa0555090:three_prime_UTR 15.0%
!! ATAATTAAGATGATTAAGAA+GGG + chr4_1:52816746-52816765 Msa0555090:three_prime_UTR 15.0%
!!! TAATACACATATTTGGTTTT+AGG - chr4_1:52815131-52815150 None:intergenic 20.0%
!!! TATAATGTATTGATGAATGA+AGG + chr4_1:52815652-52815671 Msa0555090:intron 20.0%
!!! TATATATTGTTATTGATACG+TGG + chr4_1:52815903-52815922 Msa0555090:intron 20.0%
!! CTATATAATAGGCTTATAAA+TGG + chr4_1:52816119-52816138 Msa0555090:intron 20.0%
!!! GATATTTTATTTTTAGAAGG+TGG + chr4_1:52816662-52816681 Msa0555090:three_prime_UTR 20.0%
!!! GGTGGATATTTTTATTTTTT+TGG + chr4_1:52816680-52816699 Msa0555090:three_prime_UTR 20.0%
!!! CAATGGTAAGAAATTTTATT+AGG + chr4_1:52816799-52816818 Msa0555090:three_prime_UTR 20.0%
!! TGATGTGTTTACTTTATATA+AGG + chr4_1:52816898-52816917 Msa0555090:three_prime_UTR 20.0%
!! GATGTGTTTACTTTATATAA+GGG + chr4_1:52816899-52816918 Msa0555090:three_prime_UTR 20.0%
!! ATGTGTTTACTTTATATAAG+GGG + chr4_1:52816900-52816919 Msa0555090:three_prime_UTR 20.0%
!! CAACAAATACTAACAAAAAA+AGG - chr4_1:52817086-52817105 None:intergenic 20.0%
!!! TCTTTATTTGACGTATTAAT+TGG + chr4_1:52817302-52817321 Msa0555090:three_prime_UTR 20.0%
!! ATCACACATTTCATTTGTTA+AGG + chr4_1:52814391-52814410 Msa0555090:five_prime_UTR 25.0%
!! ATCTCTTTTTCTCTCAAATA+TGG + chr4_1:52814458-52814477 Msa0555090:exon 25.0%
!!! TTTTAAGGTTAGGCATTTTT+TGG - chr4_1:52814548-52814567 None:intergenic 25.0%
! AATGATGAAGATGACAATAA+TGG + chr4_1:52815008-52815027 Msa0555090:CDS 25.0%
! AACCAAATATGTGTATTACA+TGG + chr4_1:52815133-52815152 Msa0555090:intron 25.0%
!! TTATTAATTCTAGCTAGCTA+TGG + chr4_1:52815216-52815235 Msa0555090:intron 25.0%
!!! ATCTTTGAAGATTTTGTCTT+GGG - chr4_1:52815780-52815799 None:intergenic 25.0%
! GAAAGTTTAGACAGAATTAT+AGG + chr4_1:52815956-52815975 Msa0555090:intron 25.0%
! AATATCAAGAATGACAACAT+AGG - chr4_1:52816648-52816667 None:intergenic 25.0%
! TCTTACTACTAAAATTTGGA+AGG + chr4_1:52816715-52816734 Msa0555090:three_prime_UTR 25.0%
! TTAGGAAATTGAATTGATGA+AGG + chr4_1:52816817-52816836 Msa0555090:three_prime_UTR 25.0%
! TAAAGATGATTACATATCCA+TGG - chr4_1:52817288-52817307 None:intergenic 25.0%
AAAGACATGTATGTATGTGA+AGG - chr4_1:52814241-52814260 None:intergenic 30.0%
! TTTTGGTGAGAAGAAAATTC+AGG - chr4_1:52814531-52814550 None:intergenic 30.0%
! GAAAAGTTTGAAGCATTACT+TGG - chr4_1:52814579-52814598 None:intergenic 30.0%
AGCCATGTAATACACATATT+TGG - chr4_1:52815138-52815157 None:intergenic 30.0%
TTACATTGAACATGACATGA+TGG + chr4_1:52815538-52815557 Msa0555090:intron 30.0%
!! CATCTTTGAAGATTTTGTCT+TGG - chr4_1:52815781-52815800 None:intergenic 30.0%
CAAGACAAAATCTTCAAAGA+TGG + chr4_1:52815779-52815798 Msa0555090:CDS 30.0%
GGTGAAATGAGAATATGTTA+AGG - chr4_1:52815815-52815834 None:intergenic 30.0%
ATACTACATGACAGTGTAAA+AGG - chr4_1:52815836-52815855 None:intergenic 30.0%
GTCATTAAGATTACTACTAG+TGG + chr4_1:52816351-52816370 Msa0555090:intron 30.0%
!! TGTTCTTGATGTTTTGTTGT+TGG - chr4_1:52816510-52816529 None:intergenic 30.0%
! TCTTATCAATGTTTGAGACA+TGG + chr4_1:52816606-52816625 Msa0555090:exon 30.0%
ACTACTAAAATTTGGAAGGA+AGG + chr4_1:52816719-52816738 Msa0555090:three_prime_UTR 30.0%
GACAAAAAGTTGTAATGCAA+TGG + chr4_1:52816782-52816801 Msa0555090:three_prime_UTR 30.0%
GAGAAGAAATAAACAGTACT+TGG + chr4_1:52816992-52817011 Msa0555090:three_prime_UTR 30.0%
AAGAAATAAACAGTACTTGG+TGG + chr4_1:52816995-52817014 Msa0555090:three_prime_UTR 30.0%
GGGTTTATGTAATGTAATGT+TGG + chr4_1:52817046-52817065 Msa0555090:three_prime_UTR 30.0%
!!! TGCTGTTTTTCAATTCAAGT+TGG + chr4_1:52817141-52817160 Msa0555090:three_prime_UTR 30.0%
! AATGAAGAAGCATGTAGCAT+AGG - chr4_1:52814273-52814292 None:intergenic 35.0%
! ATGTTTGGAATTGGAACCTT+TGG - chr4_1:52814495-52814514 None:intergenic 35.0%
!! TACTTGGTGAGATGGTTTTA+AGG - chr4_1:52814563-52814582 None:intergenic 35.0%
!!! TGAAGGGTTTTGTTGATTGT+TGG - chr4_1:52814601-52814620 None:intergenic 35.0%
ACAATCAACAAAACCCTTCA+TGG + chr4_1:52814601-52814620 Msa0555090:CDS 35.0%
CAATCAACAAAACCCTTCAT+GGG + chr4_1:52814602-52814621 Msa0555090:CDS 35.0%
ATGGAGACAATGAGTTTGAA+AGG + chr4_1:52814736-52814755 Msa0555090:CDS 35.0%
TTAGATGACAATGTAGCTGA+TGG - chr4_1:52814840-52814859 None:intergenic 35.0%
TATGATCAGAAGGAGTTGAA+GGG - chr4_1:52814869-52814888 None:intergenic 35.0%
TTATGATCAGAAGGAGTTGA+AGG - chr4_1:52814870-52814889 None:intergenic 35.0%
TTGATGCTGTTATGATCAGA+AGG - chr4_1:52814879-52814898 None:intergenic 35.0%
ATTGAAGCATGAATCTGATG+AGG + chr4_1:52814944-52814963 Msa0555090:CDS 35.0%
GAATCTGATGAGGATGAAAT+TGG + chr4_1:52814954-52814973 Msa0555090:CDS 35.0%
TGAAATTGGTGAATGCAAAC+AGG + chr4_1:52814968-52814987 Msa0555090:CDS 35.0%
AAAAATTACTGACCCCAAGA+GGG + chr4_1:52815055-52815074 Msa0555090:CDS 35.0%
! GTTCCTTTTCATTCATGTGA+TGG + chr4_1:52815376-52815395 Msa0555090:intron 35.0%
TCACCATCACATGAATGAAA+AGG - chr4_1:52815382-52815401 None:intergenic 35.0%
TTGCATATTTGGATCATCAG+AGG + chr4_1:52815702-52815721 Msa0555090:CDS 35.0%
TAGGCTTATAAATGGAACAG+AGG + chr4_1:52816127-52816146 Msa0555090:intron 35.0%
AATGGAACAGAGGATAACAA+CGG + chr4_1:52816137-52816156 Msa0555090:intron 35.0%
!!! CGGAATTAGCTTCTTTTTTG+TGG + chr4_1:52816157-52816176 Msa0555090:intron 35.0%
CAGAGCAATTATTAGAGAAG+AGG + chr4_1:52816211-52816230 Msa0555090:intron 35.0%
!! TCATCAAGAAGCATTGAAGA+GGG + chr4_1:52816456-52816475 Msa0555090:CDS 35.0%
TCATTATCACATCGTGGTTT+AGG - chr4_1:52816565-52816584 None:intergenic 35.0%
TGAGTATCATTATCACATCG+TGG - chr4_1:52816571-52816590 None:intergenic 35.0%
ATTGATGAAGGATAACGTGA+TGG + chr4_1:52816829-52816848 Msa0555090:three_prime_UTR 35.0%
TTGATGAAGGATAACGTGAT+GGG + chr4_1:52816830-52816849 Msa0555090:three_prime_UTR 35.0%
!! AAGCTTTTGCTATCAAACTG+TGG + chr4_1:52816867-52816886 Msa0555090:three_prime_UTR 35.0%
TTGATATTGATGTCACTGAG+TGG - chr4_1:52816971-52816990 None:intergenic 35.0%
!! TGTCTGCTGCTTTAACTATA+TGG + chr4_1:52817024-52817043 Msa0555090:three_prime_UTR 35.0%
!! GTCTGCTGCTTTAACTATAT+GGG + chr4_1:52817025-52817044 Msa0555090:three_prime_UTR 35.0%
!! TCTGCTGCTTTAACTATATG+GGG + chr4_1:52817026-52817045 Msa0555090:three_prime_UTR 35.0%
AGTTGGAAAACAAAGACTAG+TGG + chr4_1:52817158-52817177 Msa0555090:three_prime_UTR 35.0%
!! AAAGACTAGTGGAGTTTTGT+TGG + chr4_1:52817169-52817188 Msa0555090:three_prime_UTR 35.0%
!!! AAGACTAGTGGAGTTTTGTT+GGG + chr4_1:52817170-52817189 Msa0555090:three_prime_UTR 35.0%
AACAAATCAAAACCCATACG+AGG - chr4_1:52817234-52817253 None:intergenic 35.0%
!! ATTATTGATGCTGCTTTCCA+TGG + chr4_1:52817268-52817287 Msa0555090:three_prime_UTR 35.0%
!!! GTAGCTGGCTAGTTTGTTTT+TGG + chr4_1:52814424-52814443 Msa0555090:five_prime_UTR 40.0%
! TTTGGAATTGGAACCTTTGG+TGG - chr4_1:52814492-52814511 None:intergenic 40.0%
!! GTTGGTGTCATGTTTGGAAT+TGG - chr4_1:52814504-52814523 None:intergenic 40.0%
TTCCAAACATGACACCAACT+TGG + chr4_1:52814505-52814524 Msa0555090:CDS 40.0%
GAAGAAAATTCAGGCCAAGT+TGG - chr4_1:52814522-52814541 None:intergenic 40.0%
!!! GGTGAGATGGTTTTAAGGTT+AGG - chr4_1:52814558-52814577 None:intergenic 40.0%
! TGAAGCATTACTTGGTGAGA+TGG - chr4_1:52814571-52814590 None:intergenic 40.0%
CATCATCGATGCTTCTATGA+AGG - chr4_1:52814697-52814716 None:intergenic 40.0%
!! CTTCATAGAAGCATCGATGA+TGG + chr4_1:52814695-52814714 Msa0555090:CDS 40.0%
AACCTCGTCTACACTAAACA+TGG - chr4_1:52814784-52814803 None:intergenic 40.0%
CAGCATCAATGATGACAAAG+AGG + chr4_1:52814890-52814909 Msa0555090:CDS 40.0%
TGATGACAAAGAGGAAGATG+AGG + chr4_1:52814899-52814918 Msa0555090:CDS 40.0%
GGTGAATGCAAACAGGAAAA+TGG + chr4_1:52814975-52814994 Msa0555090:CDS 40.0%
GAATGCAAACAGGAAAATGG+AGG + chr4_1:52814978-52814997 Msa0555090:CDS 40.0%
! CTCTTGGGGTCAGTAATTTT+TGG - chr4_1:52815056-52815075 None:intergenic 40.0%
CAAAAATTACTGACCCCAAG+AGG + chr4_1:52815054-52815073 Msa0555090:CDS 40.0%
! TAGTACCTTTTGACCCTCTT+GGG - chr4_1:52815071-52815090 None:intergenic 40.0%
ATTGAAACATGAGCTAGCAG+TGG + chr4_1:52815324-52815343 Msa0555090:intron 40.0%
ACGAAGTGTGACTTCATTAC+AGG + chr4_1:52815501-52815520 Msa0555090:CDS 40.0%
! GAAGATTTTGTCTTGGGCTA+GGG - chr4_1:52815774-52815793 None:intergenic 40.0%
! TGAAGATTTTGTCTTGGGCT+AGG - chr4_1:52815775-52815794 None:intergenic 40.0%
GCAATTGCAAGCTTAGTTAG+TGG - chr4_1:52816026-52816045 None:intergenic 40.0%
!! CTCATCAAGAAGCATTGAAG+AGG + chr4_1:52816455-52816474 Msa0555090:CDS 40.0%
! ACGTGATGGGAGAATATAAG+TGG + chr4_1:52816843-52816862 Msa0555090:three_prime_UTR 40.0%
ACCACTCAAAGTTGCATGAT+TGG + chr4_1:52816923-52816942 Msa0555090:three_prime_UTR 40.0%
TCCAATCATGCAACTTTGAG+TGG - chr4_1:52816927-52816946 None:intergenic 40.0%
!! TAAACAGTACTTGGTGGTGT+TGG + chr4_1:52817001-52817020 Msa0555090:three_prime_UTR 40.0%
!! GTTTTGTTGGGATGGATGAA+AGG + chr4_1:52817182-52817201 Msa0555090:three_prime_UTR 40.0%
AGTGAAATCTTGCCTCGTAT+GGG + chr4_1:52817219-52817238 Msa0555090:three_prime_UTR 40.0%
ATGTATGTGAAGGAGCCAGA+GGG - chr4_1:52814231-52814250 None:intergenic 45.0%
TATGTATGTGAAGGAGCCAG+AGG - chr4_1:52814232-52814251 None:intergenic 45.0%
TAAGGCAATTCCACTGTAGC+TGG + chr4_1:52814409-52814428 Msa0555090:five_prime_UTR 45.0%
AACAAACTAGCCAGCTACAG+TGG - chr4_1:52814422-52814441 None:intergenic 45.0%
TATGGCTCAATTGCCACCAA+AGG + chr4_1:52814476-52814495 Msa0555090:exon 45.0%
TCAACAAAACCCTTCATGGG+TGG + chr4_1:52814605-52814624 Msa0555090:CDS 45.0%
ATTTCTCGTCCACCAGAAGA+GGG + chr4_1:52814640-52814659 Msa0555090:CDS 45.0%
TATGAAGGTCACTGAGTCAC+TGG - chr4_1:52814682-52814701 None:intergenic 45.0%
GTCCATGTTTAGTGTAGACG+AGG + chr4_1:52814779-52814798 Msa0555090:CDS 45.0%
TTAGTGTAGACGAGGTTTCG+AGG + chr4_1:52814787-52814806 Msa0555090:CDS 45.0%
TGACAAAGAGGAAGATGAGG+AGG + chr4_1:52814902-52814921 Msa0555090:CDS 45.0%
!! GGGGTCAGTAATTTTTGGAC+TGG - chr4_1:52815051-52815070 None:intergenic 45.0%
! AGTACCTTTTGACCCTCTTG+GGG - chr4_1:52815070-52815089 None:intergenic 45.0%
! GTAGTACCTTTTGACCCTCT+TGG - chr4_1:52815072-52815091 None:intergenic 45.0%
CTGCACAAAGATCAAGAGTG+AGG + chr4_1:52815452-52815471 Msa0555090:CDS 45.0%
AAGTATCAGTGCTGTCACCA+CGG + chr4_1:52815678-52815697 Msa0555090:CDS 45.0%
TGATCCAAATATGCAACCCG+TGG - chr4_1:52815698-52815717 None:intergenic 45.0%
TTGTGGAACTTTGCACCGTA+CGG + chr4_1:52816174-52816193 Msa0555090:intron 45.0%
GATTCAACTCATGTGCCGTA+CGG - chr4_1:52816192-52816211 None:intergenic 45.0%
CATTGAAGAGGGAGATAGAG+AGG + chr4_1:52816467-52816486 Msa0555090:CDS 45.0%
TCACATCGTGGTTTAGGTGA+TGG - chr4_1:52816559-52816578 None:intergenic 45.0%
!!! CTAGTGGAGTTTTGTTGGGA+TGG + chr4_1:52817174-52817193 Msa0555090:three_prime_UTR 45.0%
TGTTGGGATGGATGAAAGGA+AGG + chr4_1:52817186-52817205 Msa0555090:three_prime_UTR 45.0%
CAGTGAAATCTTGCCTCGTA+TGG + chr4_1:52817218-52817237 Msa0555090:three_prime_UTR 45.0%
!!! AATTTAAAATAAATAAGTAA+AGG - chr4_1:52815283-52815302 None:intergenic 5.0%
!!! ATTTATTTTAAATTTTTTTC+TGG + chr4_1:52815289-52815308 Msa0555090:intron 5.0%
!! GGCCAAGTTGGTGTCATGTT+TGG - chr4_1:52814510-52814529 None:intergenic 50.0%
GAAACTCGTCCACCCATGAA+GGG - chr4_1:52814617-52814636 None:intergenic 50.0%
AGAAACTCGTCCACCCATGA+AGG - chr4_1:52814618-52814637 None:intergenic 50.0%
GATTTCTCGTCCACCAGAAG+AGG + chr4_1:52814639-52814658 Msa0555090:CDS 50.0%
TTTCTCGTCCACCAGAAGAG+GGG + chr4_1:52814641-52814660 Msa0555090:CDS 50.0%
!! TGATGGAGCATTGTCGTCGT+AGG + chr4_1:52814712-52814731 Msa0555090:CDS 50.0%
!! GATGGAGCATTGTCGTCGTA+GGG + chr4_1:52814713-52814732 Msa0555090:CDS 50.0%
! AATGTAGCTGATGGTGCTGG+TGG - chr4_1:52814831-52814850 None:intergenic 50.0%
! GACAATGTAGCTGATGGTGC+TGG - chr4_1:52814834-52814853 None:intergenic 50.0%
ACATGAGCTAGCAGTGGTGA+TGG + chr4_1:52815330-52815349 Msa0555090:intron 50.0%
AGTATCAGTGCTGTCACCAC+GGG + chr4_1:52815679-52815698 Msa0555090:CDS 50.0%
! GTCACCACGGGTTGCATATT+TGG + chr4_1:52815691-52815710 Msa0555090:CDS 50.0%
CTAGTTGCAGACACAGCACA+TGG - chr4_1:52816323-52816342 None:intergenic 50.0%
!! CGTGGTTTAGGTGATGGAGA+TGG - chr4_1:52816553-52816572 None:intergenic 50.0%
! AAGAGGGGCACACAGACGTT+CGG + chr4_1:52814656-52814675 Msa0555090:CDS 55.0%
! GAGCATTGTCGTCGTAGGGA+TGG + chr4_1:52814717-52814736 Msa0555090:CDS 55.0%
CTGACCCCAAGAGGGTCAAA+AGG + chr4_1:52815063-52815082 Msa0555090:CDS 55.0%
AGAGGGAGATAGAGAGGCTG+AGG + chr4_1:52816473-52816492 Msa0555090:CDS 55.0%
TCTGTGTGCCCCTCTTCTGG+TGG - chr4_1:52814652-52814671 None:intergenic 60.0%
ACGTCTGTGTGCCCCTCTTC+TGG - chr4_1:52814655-52814674 None:intergenic 60.0%
!! TGGTGCTGGTGGTGGCATCA+TGG - chr4_1:52814820-52814839 None:intergenic 60.0%
!! GTAGCTGATGGTGCTGGTGG+TGG - chr4_1:52814828-52814847 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 52814220 52817338 52814220 ID=Msa0555090;Name=Msa0555090
chr4_1 mRNA 52814220 52817338 52814220 ID=Msa0555090-mRNA-1;Parent=Msa0555090;Name=Msa0555090-mRNA-1;_AED=0.04;_eAED=0.04;_QI=257|1|1|1|1|1|4|717|330
chr4_1 exon 52814220 52815084 52814220 ID=Msa0555090-mRNA-1:exon:15330;Parent=Msa0555090-mRNA-1
chr4_1 exon 52815432 52815522 52815432 ID=Msa0555090-mRNA-1:exon:15331;Parent=Msa0555090-mRNA-1
chr4_1 exon 52815674 52815800 52815674 ID=Msa0555090-mRNA-1:exon:15332;Parent=Msa0555090-mRNA-1
chr4_1 exon 52816455 52817338 52816455 ID=Msa0555090-mRNA-1:exon:15333;Parent=Msa0555090-mRNA-1
chr4_1 five_prime_UTR 52814220 52814476 52814220 ID=Msa0555090-mRNA-1:five_prime_utr;Parent=Msa0555090-mRNA-1
chr4_1 CDS 52814477 52815084 52814477 ID=Msa0555090-mRNA-1:cds;Parent=Msa0555090-mRNA-1
chr4_1 CDS 52815432 52815522 52815432 ID=Msa0555090-mRNA-1:cds;Parent=Msa0555090-mRNA-1
chr4_1 CDS 52815674 52815800 52815674 ID=Msa0555090-mRNA-1:cds;Parent=Msa0555090-mRNA-1
chr4_1 CDS 52816455 52816621 52816455 ID=Msa0555090-mRNA-1:cds;Parent=Msa0555090-mRNA-1
chr4_1 three_prime_UTR 52816622 52817338 52816622 ID=Msa0555090-mRNA-1:three_prime_utr;Parent=Msa0555090-mRNA-1
Gene Sequence

>Msa0555090

ATGGCTCAATTGCCACCAAAGGTTCCAATTCCAAACATGACACCAACTTGGCCTGAATTTTCTTCTCACCAAAAAATGCCTAACCTTAAAACCATCTCACCAAGTAATGCTTCAAACTTTTCCAACAATCAACAAAACCCTTCATGGGTGGACGAGTTTCTTGATTTCTCGTCCACCAGAAGAGGGGCACACAGACGTTCGGCCAGTGACTCAGTGACCTTCATAGAAGCATCGATGATGGAGCATTGTCGTCGTAGGGATGGAGACAATGAGTTTGAAAGGTTTGATGATGAACAATTGATGTCCATGTTTAGTGTAGACGAGGTTTCGAGGAATAATACCATGATGCCACCACCAGCACCATCAGCTACATTGTCATCTAACTCTAACCCTTCAACTCCTTCTGATCATAACAGCATCAATGATGACAAAGAGGAAGATGAGGAGGAAAAGATGCAACAGAAACAATTGAAGCATGAATCTGATGAGGATGAAATTGGTGAATGCAAACAGGAAAATGGAGGAGTTGTGAATGATGAAGATGACAATAATGGAAATACAACAACTTTTTCCAGTCCAAAAATTACTGACCCCAAGAGGGTCAAAAGAATCTTAGCAAATAGACAATCTGCACAAAGATCAAGAGTGAGGAAACTGCAATACATATCAGAGCTTGAACGAAGTGTGACTTCATTACAGGCTGAAGTATCAGTGCTGTCACCACGGGTTGCATATTTGGATCATCAGAGGTTGCTTCTAAATGTTGATAACAGTGCTATCAAGCAAAGAATCGCAGCCCTAGCCCAAGACAAAATCTTCAAAGATGCTCATCAAGAAGCATTGAAGAGGGAGATAGAGAGGCTGAGGCAAGTGTATCACCAACAACAAAACATCAAGAACAATGCTACAGCAGCAGTGTCACCATCTCCATCACCTAAACCACGATGTGATAATGATACTCACATTGAAAATGAACATCTTATCAATGTTTGA
Protein sequence

>Msa0555090

MAQLPPKVPIPNMTPTWPEFSSHQKMPNLKTISPSNASNFSNNQQNPSWVDEFLDFSSTRRGAHRRSASDSVTFIEASMMEHCRRRDGDNEFERFDDEQLMSMFSVDEVSRNNTMMPPPAPSATLSSNSNPSTPSDHNSINDDKEEDEEEKMQQKQLKHESDEDEIGECKQENGGVVNDEDDNNGNTTTFSSPKITDPKRVKRILANRQSAQRSRVRKLQYISELERSVTSLQAEVSVLSPRVAYLDHQRLLLNVDNSAIKQRIAALAQDKIFKDAHQEALKREIERLRQVYHQQQNIKNNATAAVSPSPSPKPRCDNDTHIENEHLINV*