AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0333760

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0333760 MtrunA17_Chr2g0331031 96.735 245 8 0 1 245 109 353 3.99e-179 495
Msa0333760 MtrunA17_Chr4g0055931 49.583 240 107 7 1 234 95 326 2.39e-64 203
Msa0333760 MtrunA17_Chr6g0488471 78.788 66 14 0 1 66 97 162 2.53e-33 122
Msa0333760 MtrunA17_Chr8g0389241 77.273 66 14 1 1 66 105 169 2.37e-31 116
Msa0333760 MtrunA17_Chr3g0092621 69.014 71 21 1 1 70 84 154 1.95e-30 111
Msa0333760 MtrunA17_Chr1g0210471 60.000 85 33 1 1 85 124 207 5.61e-30 112
Msa0333760 MtrunA17_Chr4g0062741 73.846 65 17 0 1 65 138 202 5.75e-30 113
Msa0333760 MtrunA17_Chr3g0104071 45.833 144 60 5 1 143 147 273 1.34e-29 113
Msa0333760 MtrunA17_Chr5g0429521 71.212 66 19 0 1 66 132 197 3.66e-29 111
Msa0333760 MtrunA17_Chr1g0162081 52.381 105 42 1 1 105 122 218 1.42e-28 109
Msa0333760 MtrunA17_Chr7g0241761 72.727 66 18 0 1 66 140 205 1.72e-28 109
Msa0333760 MtrunA17_Chr5g0415191 69.512 82 24 1 1 81 102 183 6.55e-28 108
Msa0333760 MtrunA17_Chr5g0436501 60.000 85 32 1 1 83 125 209 2.41e-27 105
Msa0333760 MtrunA17_Chr4g0059701 70.370 81 23 1 1 81 84 163 1.67e-26 103
Msa0333760 MtrunA17_Chr3g0135671 62.319 69 23 1 1 66 91 159 3.91e-24 96.7
Msa0333760 MtrunA17_Chr6g0452791 67.797 59 17 1 1 57 80 138 1.07e-22 91.3
Msa0333760 MtrunA17_Chr5g0409841 46.067 89 46 1 1 87 92 180 7.08e-21 87.8
Msa0333760 MtrunA17_Chr5g0440741 56.923 65 25 1 3 64 102 166 2.08e-20 87.8
Msa0333760 MtrunA17_Chr5g0440731 53.731 67 28 1 1 64 105 171 5.86e-20 86.7
Msa0333760 MtrunA17_Chr5g0409831 58.491 53 22 0 1 53 77 129 7.60e-17 77.0
Msa0333760 MtrunA17_Chr8g0364731 62.069 58 20 1 10 65 10 67 1.62e-15 72.4
Msa0333760 MtrunA17_Chr8g0365011 45.455 77 28 2 3 65 94 170 2.21e-15 73.9
Msa0333760 MtrunA17_Chr1g0192061 53.846 65 25 2 3 63 63 126 1.46e-14 69.7
Msa0333760 MtrunA17_Chr5g0432921 51.562 64 27 2 3 63 115 177 4.71e-14 69.3
Msa0333760 MtrunA17_Chr5g0432941 50.000 64 28 2 3 63 115 177 1.54e-13 67.8
Msa0333760 MtrunA17_Chr8g0370121 53.191 47 22 0 1 47 100 146 3.84e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0333760 AT3G16350.1 55.906 254 100 8 1 245 137 387 5.56e-70 220
Msa0333760 AT5G47390.1 44.322 273 107 8 1 236 96 360 9.24e-58 187
Msa0333760 AT2G38090.1 72.603 73 20 0 1 73 141 213 1.55e-31 118
Msa0333760 AT5G56840.1 73.134 67 17 1 1 66 91 157 4.28e-30 112
Msa0333760 AT5G61620.1 44.444 144 58 4 2 145 109 230 1.68e-29 113
Msa0333760 AT1G49010.1 73.846 65 17 0 1 65 134 198 2.13e-29 112
Msa0333760 AT5G58900.1 72.308 65 18 0 1 65 141 205 2.17e-29 112
Msa0333760 AT1G70000.1 69.048 84 25 1 1 84 98 180 1.56e-28 109
Msa0333760 AT1G70000.2 69.048 84 25 1 1 84 98 180 1.56e-28 109
Msa0333760 AT5G08520.1 69.231 65 20 0 1 65 118 182 1.73e-27 107
Msa0333760 AT5G04760.1 63.636 66 24 0 1 66 99 164 1.35e-26 102
Msa0333760 AT3G11280.2 70.769 65 19 0 1 65 127 191 3.04e-26 103
Msa0333760 AT3G11280.1 70.769 65 19 0 1 65 127 191 3.04e-26 103
Msa0333760 AT5G01200.1 67.692 65 21 0 1 65 146 210 1.31e-25 101
Msa0333760 AT5G05790.2 45.872 109 53 1 1 109 131 233 2.47e-25 101
Msa0333760 AT5G05790.1 45.872 109 53 1 1 109 131 233 2.47e-25 101
Msa0333760 AT1G74840.2 64.835 91 27 2 1 90 96 182 2.06e-24 97.8
Msa0333760 AT1G19000.2 65.169 89 26 2 1 89 102 185 2.88e-24 98.6
Msa0333760 AT1G19000.1 65.169 89 26 2 1 89 102 185 2.88e-24 98.6
Msa0333760 AT1G74840.1 74.286 70 18 0 1 70 96 165 4.07e-24 97.8
Msa0333760 AT3G10580.2 60.606 66 24 1 1 66 95 158 1.58e-21 90.5
Msa0333760 AT4G09450.1 58.571 70 29 0 1 70 91 160 2.12e-21 89.0
Msa0333760 AT3G10580.1 60.606 66 24 1 1 66 95 158 2.41e-21 90.5
Msa0333760 AT5G23650.1 56.061 66 29 0 1 66 120 185 9.53e-19 84.3
Msa0333760 AT3G10590.1 52.308 65 30 1 2 66 112 175 1.85e-16 75.9

Find 88 sgRNAs with CRISPR-Local

Find 218 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATCCCATTGTAGTCTTAT+TGG 0.183629 2_4:+51777149 None:intergenic
AGTCTCCTTTGCCTAGCTTT+TGG 0.260546 2_4:+51778007 None:intergenic
GGAAGAACATAGATTGTTCT+TGG 0.306357 2_4:-51778037 Msa0333760:CDS
GGAAGTAGTATTTGCTCTTC+TGG 0.308188 2_4:+51777285 None:intergenic
GAAAATGAGGCAGGTAAATA+AGG 0.320772 2_4:+51777090 None:intergenic
AGTTTGAGGCTTTCAAAGTA+TGG 0.334376 2_4:-51776782 Msa0333760:exon
TCCTTTGGAATGACTGGTAT+TGG 0.340477 2_4:+51776994 None:intergenic
CCCCTTTCCTTGAAGTTGTT+AGG 0.350389 2_4:-51776892 Msa0333760:CDS
TCTGGCATCGAAGTTGAATC+TGG 0.363477 2_4:+51777303 None:intergenic
TGGAATGCTGATTGCCTTGA+GGG 0.363738 2_4:+51776865 None:intergenic
GTAGTAACTAAGCCTTCTTT+TGG 0.378146 2_4:-51776751 Msa0333760:three_prime_UTR
GCTCAGGTTAGATAAATTCT+TGG 0.380065 2_4:-51776628 Msa0333760:three_prime_UTR
TCAACATTGACAGGTTCCTT+TGG 0.380571 2_4:+51776979 None:intergenic
TTTAGATCAGAACCGCTAAC+TGG 0.381028 2_4:+51776832 None:intergenic
TCAAAGGGAGCTCCAATTGA+TGG 0.384606 2_4:+51777060 None:intergenic
TACTTAGAAGTTAAACACTA+TGG 0.389922 2_4:+51776676 None:intergenic
GAATGTCTCATCTGAGCATT+GGG 0.393213 2_4:-51776945 Msa0333760:CDS
TCCTTTGTTGACTTCTTCAA+AGG 0.400771 2_4:+51777044 None:intergenic
AAAGCCTCAAACTGCTTGAA+TGG 0.420085 2_4:+51776792 None:intergenic
AAGACTACAATGGGATCAAA+TGG 0.426750 2_4:-51777144 Msa0333760:CDS
CGAGAGGAACATGGACCTCT+TGG 0.431558 2_4:+51776546 None:intergenic
CCTTTGTTGACTTCTTCAAA+GGG 0.434334 2_4:+51777045 None:intergenic
TCCTAACAACTTCAAGGAAA+GGG 0.440079 2_4:+51776891 None:intergenic
TCTTCAGATGTAGCTTCCAT+TGG 0.445737 2_4:+51777186 None:intergenic
AAAAGCTAGGCAAAGGAGAC+TGG 0.448710 2_4:-51778005 Msa0333760:CDS
GGGAAGAATCCTTGCGTCAT+TGG 0.453748 2_4:+51777114 None:intergenic
ACAGGTTCCTTTGGAATGAC+TGG 0.453964 2_4:+51776988 None:intergenic
CACATGGTGTTATGTAGCTC+AGG 0.457543 2_4:-51776644 Msa0333760:three_prime_UTR
GAACATAGATTGTTCTTGGT+TGG 0.461435 2_4:-51778033 Msa0333760:CDS
CATGACTTGCTACTTGAGTA+GGG 0.462555 2_4:+51777947 None:intergenic
AACGCCATTCAAGCAGTTTG+AGG 0.463617 2_4:-51776796 Msa0333760:exon
GCATGACTTGCTACTTGAGT+AGG 0.464793 2_4:+51777946 None:intergenic
GAGGCCAATAAGACTACAAT+GGG 0.480586 2_4:-51777153 Msa0333760:CDS
GAATGAATACTGACCACATC+AGG 0.481403 2_4:+51777853 None:intergenic
AGCTCCAATTGATGGCCATA+TGG 0.483931 2_4:+51777068 None:intergenic
GGGCTCTCCTAACAACTTCA+AGG 0.485368 2_4:+51776885 None:intergenic
CATGCAAATGCTCCAGTTAG+CGG 0.489459 2_4:-51776844 Msa0333760:CDS
ACAAGTTCATCAACATTGAC+AGG 0.494176 2_4:+51776970 None:intergenic
GAGAACTCACAACCTTGTAA+TGG 0.500646 2_4:-51777255 Msa0333760:CDS
ATCTGAGCATTGGGGAAACA+CGG 0.503897 2_4:-51776936 Msa0333760:CDS
TTTCGACATGGCTCCTGATG+TGG 0.516858 2_4:-51777866 Msa0333760:intron
CCTAACAACTTCAAGGAAAG+GGG 0.517543 2_4:+51776892 None:intergenic
CTATGTTCTTCCTCAGTCCA+TGG 0.518156 2_4:+51778048 None:intergenic
TGATGGCCATATGGAAAATG+AGG 0.522407 2_4:+51777077 None:intergenic
AGGAGAGACATCGTATCATC+AGG 0.522529 2_4:-51777025 Msa0333760:CDS
AGGAGCCATGTCGAAAAGAC+TGG 0.523291 2_4:+51777873 None:intergenic
CGCTAACTGGAGCATTTGCA+TGG 0.525907 2_4:+51776845 None:intergenic
GGAATGTCTCATCTGAGCAT+TGG 0.531337 2_4:-51776946 Msa0333760:CDS
AATGAAAGATGGCCAAAAGA+AGG 0.531941 2_4:+51776739 None:intergenic
ATTATAGCACAAATGAAAGA+TGG 0.533034 2_4:+51776728 None:intergenic
TTGGTTGGTCTCCAAAAGCT+AGG 0.538073 2_4:-51778018 Msa0333760:CDS
ATCTGAAGAAAATGTAGAAG+AGG 0.540720 2_4:-51777172 Msa0333760:CDS
ATGAGGCAGGTAAATAAGGT+GGG 0.541274 2_4:+51777094 None:intergenic
CAAGAGGTCCATGTTCCTCT+CGG 0.542677 2_4:-51776545 Msa0333760:three_prime_UTR
CTCCTAACAACTTCAAGGAA+AGG 0.542864 2_4:+51776890 None:intergenic
TCCTGGTAGCATTACTCTGT+CGG 0.543171 2_4:+51777908 None:intergenic
GGCCATATGGAAAATGAGGC+AGG 0.543383 2_4:+51777081 None:intergenic
ATGGAATGCTGATTGCCTTG+AGG 0.546697 2_4:+51776864 None:intergenic
CAAGTCAGAATATGAACCAA+TGG 0.562551 2_4:-51777202 Msa0333760:CDS
CAAGGTTGTGAGTTCTCAGA+AGG 0.569930 2_4:+51777261 None:intergenic
GAATGACTGGTATTGGCTTG+AGG 0.572196 2_4:+51777001 None:intergenic
ACTGGAACGTCTCTTCCTCC+TGG 0.576071 2_4:+51777891 None:intergenic
AGTTGTTAGGAGAGCCCTCA+AGG 0.579322 2_4:-51776879 Msa0333760:CDS
TCTGAGCATTGGGGAAACAC+GGG 0.582582 2_4:-51776935 Msa0333760:CDS
AATGAGGCAGGTAAATAAGG+TGG 0.582975 2_4:+51777093 None:intergenic
GTCAATGTTGATGAACTTGT+GGG 0.584457 2_4:-51776967 Msa0333760:CDS
TAGCCAAACCGAGAGGAACA+TGG 0.589318 2_4:+51776537 None:intergenic
AGAGGCCAATAAGACTACAA+TGG 0.591083 2_4:-51777154 Msa0333760:CDS
TGTCAATGTTGATGAACTTG+TGG 0.593101 2_4:-51776968 Msa0333760:CDS
GGTTGTGAGTTCTCAGAAGG+TGG 0.602477 2_4:+51777264 None:intergenic
GGTCTCCAAAAGCTAGGCAA+AGG 0.614877 2_4:-51778012 Msa0333760:CDS
CTAACAACTTCAAGGAAAGG+GGG 0.629370 2_4:+51776893 None:intergenic
CTAGGCAAAGGAGACTGGCG+AGG 0.631792 2_4:-51778000 Msa0333760:CDS
CTAAGTAGTAAGAAGTCACA+TGG 0.631808 2_4:-51776660 Msa0333760:three_prime_UTR
CCCTTTGAAGAAGTCAACAA+AGG 0.636313 2_4:-51777045 Msa0333760:CDS
TTGAGAGACAGATTTAGTGA+TGG 0.636747 2_4:+51777222 None:intergenic
TCCGACAGAGTAATGCTACC+AGG 0.640061 2_4:-51777909 Msa0333760:CDS
GCCAATACCAGTCATTCCAA+AGG 0.641238 2_4:-51776995 Msa0333760:CDS
ATGACTTGCTACTTGAGTAG+GGG 0.646346 2_4:+51777948 None:intergenic
GGATTCACACCAATGACGCA+AGG 0.652345 2_4:-51777123 Msa0333760:CDS
TAGGCAAAGGAGACTGGCGA+GGG 0.653435 2_4:-51777999 Msa0333760:CDS
TAACAACTTCAAGGAAAGGG+GGG 0.654354 2_4:+51776894 None:intergenic
AATAATCAAATATAGCCAAG+AGG 0.656600 2_4:-51776561 Msa0333760:three_prime_UTR
TTTAGGTGTTCCATGGACTG+AGG 0.681364 2_4:-51778058 None:intergenic
AGCGGTTCTGATCTAAACAG+TGG 0.708017 2_4:-51776826 Msa0333760:CDS
ACAATTATAGCCAAACCGAG+AGG 0.730520 2_4:+51776530 None:intergenic
AATGTCTCATCTGAGCATTG+GGG 0.733687 2_4:-51776944 Msa0333760:CDS
GACAGAGTAATGCTACCAGG+AGG 0.755723 2_4:-51777906 Msa0333760:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAAAAGTTAATAAGAT+AGG + chr2_4:51777251-51777270 None:intergenic 10.0%
!! AATAAAAAAGTTAATAAGAT+AGG + chr2_4:51777251-51777270 None:intergenic 10.0%
!! CATAGATAAAAAATATGTTA+TGG + chr2_4:51776917-51776936 None:intergenic 15.0%
!! CATAGATAAAAAATATGTTA+TGG + chr2_4:51776917-51776936 None:intergenic 15.0%
!! TTATGGTAATTAGCAAATAA+AGG + chr2_4:51777150-51777169 None:intergenic 20.0%
!!! TATTTGCTAATTACCATAAA+CGG - chr2_4:51777151-51777170 Msa0333760:CDS 20.0%
!! TTATGGTAATTAGCAAATAA+AGG + chr2_4:51777150-51777169 None:intergenic 20.0%
!!! TATTTGCTAATTACCATAAA+CGG - chr2_4:51777151-51777170 Msa0333760:CDS 20.0%
! CATATATATTGCTTATAAGC+TGG + chr2_4:51776882-51776901 None:intergenic 25.0%
!!! TATGTTTTGAGAAAATACCT+TGG - chr2_4:51776933-51776952 Msa0333760:CDS 25.0%
! ATAACTGTACTCTGTTTATA+TGG + chr2_4:51776975-51776994 None:intergenic 25.0%
!! ATTTGCTAATTACCATAAAC+GGG - chr2_4:51777152-51777171 Msa0333760:CDS 25.0%
! ATTATAGCACAAATGAAAGA+TGG + chr2_4:51777871-51777890 None:intergenic 25.0%
! TACTTAGAAGTTAAACACTA+TGG + chr2_4:51777923-51777942 None:intergenic 25.0%
! AATAATCAAATATAGCCAAG+AGG - chr2_4:51778035-51778054 Msa0333760:CDS 25.0%
! CATATATATTGCTTATAAGC+TGG + chr2_4:51776882-51776901 None:intergenic 25.0%
!!! TATGTTTTGAGAAAATACCT+TGG - chr2_4:51776933-51776952 Msa0333760:CDS 25.0%
! ATAACTGTACTCTGTTTATA+TGG + chr2_4:51776975-51776994 None:intergenic 25.0%
!! ATTTGCTAATTACCATAAAC+GGG - chr2_4:51777152-51777171 Msa0333760:CDS 25.0%
! ATTATAGCACAAATGAAAGA+TGG + chr2_4:51777871-51777890 None:intergenic 25.0%
! TACTTAGAAGTTAAACACTA+TGG + chr2_4:51777923-51777942 None:intergenic 25.0%
! AATAATCAAATATAGCCAAG+AGG - chr2_4:51778035-51778054 Msa0333760:CDS 25.0%
ATCTGAAGAAAATGTAGAAG+AGG - chr2_4:51777424-51777443 Msa0333760:intron 30.0%
ATCTGAAGAAAATGTAGAAG+AGG - chr2_4:51777424-51777443 Msa0333760:intron 30.0%
GGAAGAACATAGATTGTTCT+TGG - chr2_4:51776559-51776578 Msa0333760:three_prime_UTR 35.0%
GAACATAGATTGTTCTTGGT+TGG - chr2_4:51776563-51776582 Msa0333760:three_prime_UTR 35.0%
!! CACGTAATTTTGTTGTGTCA+AGG - chr2_4:51776624-51776643 Msa0333760:three_prime_UTR 35.0%
! GTAGTGGTGTTAATAAGGAT+AGG + chr2_4:51776858-51776877 None:intergenic 35.0%
! AGAAAATACCTTGGTGTGTT+TGG - chr2_4:51776942-51776961 Msa0333760:CDS 35.0%
TTCAATATCCAGTTACAACG+AGG + chr2_4:51777183-51777202 None:intergenic 35.0%
! GGTTGTGATTTTCCATTACA+AGG + chr2_4:51777356-51777375 None:intergenic 35.0%
TTGAGAGACAGATTTAGTGA+TGG + chr2_4:51777377-51777396 None:intergenic 35.0%
CAAGTCAGAATATGAACCAA+TGG - chr2_4:51777394-51777413 Msa0333760:intron 35.0%
!! TGATCCCATTGTAGTCTTAT+TGG + chr2_4:51777450-51777469 None:intergenic 35.0%
AAGACTACAATGGGATCAAA+TGG - chr2_4:51777452-51777471 Msa0333760:intron 35.0%
GAAAATGAGGCAGGTAAATA+AGG + chr2_4:51777509-51777528 None:intergenic 35.0%
! TTTTCCATATGGCCATCAAT+TGG - chr2_4:51777524-51777543 Msa0333760:intron 35.0%
!! CCTTTGTTGACTTCTTCAAA+GGG + chr2_4:51777554-51777573 None:intergenic 35.0%
!! TCCTTTGTTGACTTCTTCAA+AGG + chr2_4:51777555-51777574 None:intergenic 35.0%
ACAAGTTCATCAACATTGAC+AGG + chr2_4:51777629-51777648 None:intergenic 35.0%
! TGTCAATGTTGATGAACTTG+TGG - chr2_4:51777628-51777647 Msa0333760:intron 35.0%
! GTCAATGTTGATGAACTTGT+GGG - chr2_4:51777629-51777648 Msa0333760:intron 35.0%
TCCTAACAACTTCAAGGAAA+GGG + chr2_4:51777708-51777727 None:intergenic 35.0%
! AGTTTGAGGCTTTCAAAGTA+TGG - chr2_4:51777814-51777833 Msa0333760:intron 35.0%
! GTAGTAACTAAGCCTTCTTT+TGG - chr2_4:51777845-51777864 Msa0333760:intron 35.0%
AATGAAAGATGGCCAAAAGA+AGG + chr2_4:51777860-51777879 None:intergenic 35.0%
CTAAGTAGTAAGAAGTCACA+TGG - chr2_4:51777936-51777955 Msa0333760:CDS 35.0%
GCTCAGGTTAGATAAATTCT+TGG - chr2_4:51777968-51777987 Msa0333760:CDS 35.0%
GGAAGAACATAGATTGTTCT+TGG - chr2_4:51776559-51776578 Msa0333760:three_prime_UTR 35.0%
GAACATAGATTGTTCTTGGT+TGG - chr2_4:51776563-51776582 Msa0333760:three_prime_UTR 35.0%
!! CACGTAATTTTGTTGTGTCA+AGG - chr2_4:51776624-51776643 Msa0333760:three_prime_UTR 35.0%
! GTAGTGGTGTTAATAAGGAT+AGG + chr2_4:51776858-51776877 None:intergenic 35.0%
! AGAAAATACCTTGGTGTGTT+TGG - chr2_4:51776942-51776961 Msa0333760:CDS 35.0%
TTCAATATCCAGTTACAACG+AGG + chr2_4:51777183-51777202 None:intergenic 35.0%
! GGTTGTGATTTTCCATTACA+AGG + chr2_4:51777356-51777375 None:intergenic 35.0%
TTGAGAGACAGATTTAGTGA+TGG + chr2_4:51777377-51777396 None:intergenic 35.0%
CAAGTCAGAATATGAACCAA+TGG - chr2_4:51777394-51777413 Msa0333760:intron 35.0%
!! TGATCCCATTGTAGTCTTAT+TGG + chr2_4:51777450-51777469 None:intergenic 35.0%
AAGACTACAATGGGATCAAA+TGG - chr2_4:51777452-51777471 Msa0333760:intron 35.0%
GAAAATGAGGCAGGTAAATA+AGG + chr2_4:51777509-51777528 None:intergenic 35.0%
! TTTTCCATATGGCCATCAAT+TGG - chr2_4:51777524-51777543 Msa0333760:intron 35.0%
!! CCTTTGTTGACTTCTTCAAA+GGG + chr2_4:51777554-51777573 None:intergenic 35.0%
!! TCCTTTGTTGACTTCTTCAA+AGG + chr2_4:51777555-51777574 None:intergenic 35.0%
ACAAGTTCATCAACATTGAC+AGG + chr2_4:51777629-51777648 None:intergenic 35.0%
! TGTCAATGTTGATGAACTTG+TGG - chr2_4:51777628-51777647 Msa0333760:intron 35.0%
! GTCAATGTTGATGAACTTGT+GGG - chr2_4:51777629-51777648 Msa0333760:intron 35.0%
TCCTAACAACTTCAAGGAAA+GGG + chr2_4:51777708-51777727 None:intergenic 35.0%
! AGTTTGAGGCTTTCAAAGTA+TGG - chr2_4:51777814-51777833 Msa0333760:intron 35.0%
! GTAGTAACTAAGCCTTCTTT+TGG - chr2_4:51777845-51777864 Msa0333760:intron 35.0%
AATGAAAGATGGCCAAAAGA+AGG + chr2_4:51777860-51777879 None:intergenic 35.0%
CTAAGTAGTAAGAAGTCACA+TGG - chr2_4:51777936-51777955 Msa0333760:CDS 35.0%
GCTCAGGTTAGATAAATTCT+TGG - chr2_4:51777968-51777987 Msa0333760:CDS 35.0%
!! ATGACTTGCTACTTGAGTAG+GGG + chr2_4:51776651-51776670 None:intergenic 40.0%
!! CATGACTTGCTACTTGAGTA+GGG + chr2_4:51776652-51776671 None:intergenic 40.0%
GAATGAATACTGACCACATC+AGG + chr2_4:51776746-51776765 None:intergenic 40.0%
! GTGGTGTTAATAAGGATAGG+AGG + chr2_4:51776855-51776874 None:intergenic 40.0%
! CTGGTGTAGTGGTGTTAATA+AGG + chr2_4:51776863-51776882 None:intergenic 40.0%
TTGCTTATAAGCTGGTGTAG+TGG + chr2_4:51776874-51776893 None:intergenic 40.0%
TGAGAAACTCAACAGAGTCA+AGG + chr2_4:51777060-51777079 None:intergenic 40.0%
GGAAGTAGTATTTGCTCTTC+TGG + chr2_4:51777314-51777333 None:intergenic 40.0%
GAGAACTCACAACCTTGTAA+TGG - chr2_4:51777341-51777360 Msa0333760:intron 40.0%
TCTTCAGATGTAGCTTCCAT+TGG + chr2_4:51777413-51777432 None:intergenic 40.0%
AGAGGCCAATAAGACTACAA+TGG - chr2_4:51777442-51777461 Msa0333760:intron 40.0%
GAGGCCAATAAGACTACAAT+GGG - chr2_4:51777443-51777462 Msa0333760:intron 40.0%
ATGAGGCAGGTAAATAAGGT+GGG + chr2_4:51777505-51777524 None:intergenic 40.0%
AATGAGGCAGGTAAATAAGG+TGG + chr2_4:51777506-51777525 None:intergenic 40.0%
! TACCTGCCTCATTTTCCATA+TGG - chr2_4:51777513-51777532 Msa0333760:intron 40.0%
TGATGGCCATATGGAAAATG+AGG + chr2_4:51777522-51777541 None:intergenic 40.0%
! CCCTTTGAAGAAGTCAACAA+AGG - chr2_4:51777551-51777570 Msa0333760:intron 40.0%
!! TCCTTTGGAATGACTGGTAT+TGG + chr2_4:51777605-51777624 None:intergenic 40.0%
TCAACATTGACAGGTTCCTT+TGG + chr2_4:51777620-51777639 None:intergenic 40.0%
GAATGTCTCATCTGAGCATT+GGG - chr2_4:51777651-51777670 Msa0333760:intron 40.0%
! AATGTCTCATCTGAGCATTG+GGG - chr2_4:51777652-51777671 Msa0333760:intron 40.0%
TAACAACTTCAAGGAAAGGG+GGG + chr2_4:51777705-51777724 None:intergenic 40.0%
CTAACAACTTCAAGGAAAGG+GGG + chr2_4:51777706-51777725 None:intergenic 40.0%
CCTAACAACTTCAAGGAAAG+GGG + chr2_4:51777707-51777726 None:intergenic 40.0%
CTCCTAACAACTTCAAGGAA+AGG + chr2_4:51777709-51777728 None:intergenic 40.0%
TTTAGATCAGAACCGCTAAC+TGG + chr2_4:51777767-51777786 None:intergenic 40.0%
AAAGCCTCAAACTGCTTGAA+TGG + chr2_4:51777807-51777826 None:intergenic 40.0%
!! ATGACTTGCTACTTGAGTAG+GGG + chr2_4:51776651-51776670 None:intergenic 40.0%
!! CATGACTTGCTACTTGAGTA+GGG + chr2_4:51776652-51776671 None:intergenic 40.0%
GAATGAATACTGACCACATC+AGG + chr2_4:51776746-51776765 None:intergenic 40.0%
! GTGGTGTTAATAAGGATAGG+AGG + chr2_4:51776855-51776874 None:intergenic 40.0%
! CTGGTGTAGTGGTGTTAATA+AGG + chr2_4:51776863-51776882 None:intergenic 40.0%
TTGCTTATAAGCTGGTGTAG+TGG + chr2_4:51776874-51776893 None:intergenic 40.0%
TGAGAAACTCAACAGAGTCA+AGG + chr2_4:51777060-51777079 None:intergenic 40.0%
GGAAGTAGTATTTGCTCTTC+TGG + chr2_4:51777314-51777333 None:intergenic 40.0%
GAGAACTCACAACCTTGTAA+TGG - chr2_4:51777341-51777360 Msa0333760:intron 40.0%
TCTTCAGATGTAGCTTCCAT+TGG + chr2_4:51777413-51777432 None:intergenic 40.0%
AGAGGCCAATAAGACTACAA+TGG - chr2_4:51777442-51777461 Msa0333760:intron 40.0%
GAGGCCAATAAGACTACAAT+GGG - chr2_4:51777443-51777462 Msa0333760:intron 40.0%
ATGAGGCAGGTAAATAAGGT+GGG + chr2_4:51777505-51777524 None:intergenic 40.0%
AATGAGGCAGGTAAATAAGG+TGG + chr2_4:51777506-51777525 None:intergenic 40.0%
! TACCTGCCTCATTTTCCATA+TGG - chr2_4:51777513-51777532 Msa0333760:intron 40.0%
TGATGGCCATATGGAAAATG+AGG + chr2_4:51777522-51777541 None:intergenic 40.0%
! CCCTTTGAAGAAGTCAACAA+AGG - chr2_4:51777551-51777570 Msa0333760:intron 40.0%
!! TCCTTTGGAATGACTGGTAT+TGG + chr2_4:51777605-51777624 None:intergenic 40.0%
TCAACATTGACAGGTTCCTT+TGG + chr2_4:51777620-51777639 None:intergenic 40.0%
GAATGTCTCATCTGAGCATT+GGG - chr2_4:51777651-51777670 Msa0333760:intron 40.0%
! AATGTCTCATCTGAGCATTG+GGG - chr2_4:51777652-51777671 Msa0333760:intron 40.0%
TAACAACTTCAAGGAAAGGG+GGG + chr2_4:51777705-51777724 None:intergenic 40.0%
CTAACAACTTCAAGGAAAGG+GGG + chr2_4:51777706-51777725 None:intergenic 40.0%
CCTAACAACTTCAAGGAAAG+GGG + chr2_4:51777707-51777726 None:intergenic 40.0%
CTCCTAACAACTTCAAGGAA+AGG + chr2_4:51777709-51777728 None:intergenic 40.0%
TTTAGATCAGAACCGCTAAC+TGG + chr2_4:51777767-51777786 None:intergenic 40.0%
AAAGCCTCAAACTGCTTGAA+TGG + chr2_4:51777807-51777826 None:intergenic 40.0%
CTATGTTCTTCCTCAGTCCA+TGG + chr2_4:51776551-51776570 None:intergenic 45.0%
!! TTGGTTGGTCTCCAAAAGCT+AGG - chr2_4:51776578-51776597 Msa0333760:three_prime_UTR 45.0%
!! AGTCTCCTTTGCCTAGCTTT+TGG + chr2_4:51776592-51776611 None:intergenic 45.0%
AAAAGCTAGGCAAAGGAGAC+TGG - chr2_4:51776591-51776610 Msa0333760:three_prime_UTR 45.0%
!! GCATGACTTGCTACTTGAGT+AGG + chr2_4:51776653-51776672 None:intergenic 45.0%
TCCTGGTAGCATTACTCTGT+CGG + chr2_4:51776691-51776710 None:intergenic 45.0%
! ACGTTCCAGTCTTTTCGACA+TGG - chr2_4:51776718-51776737 Msa0333760:three_prime_UTR 45.0%
GATTCAAGCCAAACACACCA+AGG + chr2_4:51776953-51776972 None:intergenic 45.0%
AACGAGGATGAACCCGTTTA+TGG + chr2_4:51777167-51777186 None:intergenic 45.0%
! TCTGGCATCGAAGTTGAATC+TGG + chr2_4:51777296-51777315 None:intergenic 45.0%
CAAGGTTGTGAGTTCTCAGA+AGG + chr2_4:51777338-51777357 None:intergenic 45.0%
! AGCTCCAATTGATGGCCATA+TGG + chr2_4:51777531-51777550 None:intergenic 45.0%
TCAAAGGGAGCTCCAATTGA+TGG + chr2_4:51777539-51777558 None:intergenic 45.0%
AGGAGAGACATCGTATCATC+AGG - chr2_4:51777571-51777590 Msa0333760:intron 45.0%
!! GAATGACTGGTATTGGCTTG+AGG + chr2_4:51777598-51777617 None:intergenic 45.0%
GCCAATACCAGTCATTCCAA+AGG - chr2_4:51777601-51777620 Msa0333760:intron 45.0%
!! ACAGGTTCCTTTGGAATGAC+TGG + chr2_4:51777611-51777630 None:intergenic 45.0%
GGAATGTCTCATCTGAGCAT+TGG - chr2_4:51777650-51777669 Msa0333760:intron 45.0%
!! ATCTGAGCATTGGGGAAACA+CGG - chr2_4:51777660-51777679 Msa0333760:intron 45.0%
CCCCTTTCCTTGAAGTTGTT+AGG - chr2_4:51777704-51777723 Msa0333760:intron 45.0%
! TGGAATGCTGATTGCCTTGA+GGG + chr2_4:51777734-51777753 None:intergenic 45.0%
! ATGGAATGCTGATTGCCTTG+AGG + chr2_4:51777735-51777754 None:intergenic 45.0%
CATGCAAATGCTCCAGTTAG+CGG - chr2_4:51777752-51777771 Msa0333760:intron 45.0%
! AGCGGTTCTGATCTAAACAG+TGG - chr2_4:51777770-51777789 Msa0333760:intron 45.0%
AACGCCATTCAAGCAGTTTG+AGG - chr2_4:51777800-51777819 Msa0333760:intron 45.0%
!! CACATGGTGTTATGTAGCTC+AGG - chr2_4:51777952-51777971 Msa0333760:CDS 45.0%
CTATGTTCTTCCTCAGTCCA+TGG + chr2_4:51776551-51776570 None:intergenic 45.0%
!! TTGGTTGGTCTCCAAAAGCT+AGG - chr2_4:51776578-51776597 Msa0333760:three_prime_UTR 45.0%
!! AGTCTCCTTTGCCTAGCTTT+TGG + chr2_4:51776592-51776611 None:intergenic 45.0%
AAAAGCTAGGCAAAGGAGAC+TGG - chr2_4:51776591-51776610 Msa0333760:three_prime_UTR 45.0%
!! GCATGACTTGCTACTTGAGT+AGG + chr2_4:51776653-51776672 None:intergenic 45.0%
TCCTGGTAGCATTACTCTGT+CGG + chr2_4:51776691-51776710 None:intergenic 45.0%
! ACGTTCCAGTCTTTTCGACA+TGG - chr2_4:51776718-51776737 Msa0333760:three_prime_UTR 45.0%
GATTCAAGCCAAACACACCA+AGG + chr2_4:51776953-51776972 None:intergenic 45.0%
AACGAGGATGAACCCGTTTA+TGG + chr2_4:51777167-51777186 None:intergenic 45.0%
! TCTGGCATCGAAGTTGAATC+TGG + chr2_4:51777296-51777315 None:intergenic 45.0%
CAAGGTTGTGAGTTCTCAGA+AGG + chr2_4:51777338-51777357 None:intergenic 45.0%
! AGCTCCAATTGATGGCCATA+TGG + chr2_4:51777531-51777550 None:intergenic 45.0%
TCAAAGGGAGCTCCAATTGA+TGG + chr2_4:51777539-51777558 None:intergenic 45.0%
AGGAGAGACATCGTATCATC+AGG - chr2_4:51777571-51777590 Msa0333760:intron 45.0%
!! GAATGACTGGTATTGGCTTG+AGG + chr2_4:51777598-51777617 None:intergenic 45.0%
GCCAATACCAGTCATTCCAA+AGG - chr2_4:51777601-51777620 Msa0333760:intron 45.0%
!! ACAGGTTCCTTTGGAATGAC+TGG + chr2_4:51777611-51777630 None:intergenic 45.0%
GGAATGTCTCATCTGAGCAT+TGG - chr2_4:51777650-51777669 Msa0333760:intron 45.0%
!! ATCTGAGCATTGGGGAAACA+CGG - chr2_4:51777660-51777679 Msa0333760:intron 45.0%
CCCCTTTCCTTGAAGTTGTT+AGG - chr2_4:51777704-51777723 Msa0333760:intron 45.0%
! TGGAATGCTGATTGCCTTGA+GGG + chr2_4:51777734-51777753 None:intergenic 45.0%
! ATGGAATGCTGATTGCCTTG+AGG + chr2_4:51777735-51777754 None:intergenic 45.0%
CATGCAAATGCTCCAGTTAG+CGG - chr2_4:51777752-51777771 Msa0333760:intron 45.0%
! AGCGGTTCTGATCTAAACAG+TGG - chr2_4:51777770-51777789 Msa0333760:intron 45.0%
AACGCCATTCAAGCAGTTTG+AGG - chr2_4:51777800-51777819 Msa0333760:intron 45.0%
!! CACATGGTGTTATGTAGCTC+AGG - chr2_4:51777952-51777971 Msa0333760:CDS 45.0%
GGTCTCCAAAAGCTAGGCAA+AGG - chr2_4:51776584-51776603 Msa0333760:three_prime_UTR 50.0%
TCCGACAGAGTAATGCTACC+AGG - chr2_4:51776687-51776706 Msa0333760:three_prime_UTR 50.0%
GACAGAGTAATGCTACCAGG+AGG - chr2_4:51776690-51776709 Msa0333760:three_prime_UTR 50.0%
AGGAGCCATGTCGAAAAGAC+TGG + chr2_4:51776726-51776745 None:intergenic 50.0%
TTTCGACATGGCTCCTGATG+TGG - chr2_4:51776730-51776749 Msa0333760:three_prime_UTR 50.0%
GGGTTCATCCTCGTTGTAAC+TGG - chr2_4:51777172-51777191 Msa0333760:CDS 50.0%
GGTTGTGAGTTCTCAGAAGG+TGG + chr2_4:51777335-51777354 None:intergenic 50.0%
GGATTCACACCAATGACGCA+AGG - chr2_4:51777473-51777492 Msa0333760:intron 50.0%
GGGAAGAATCCTTGCGTCAT+TGG + chr2_4:51777485-51777504 None:intergenic 50.0%
GGCCATATGGAAAATGAGGC+AGG + chr2_4:51777518-51777537 None:intergenic 50.0%
!! TCTGAGCATTGGGGAAACAC+GGG - chr2_4:51777661-51777680 Msa0333760:intron 50.0%
AACTTCAAGGAAAGGGGGGA+AGG + chr2_4:51777701-51777720 None:intergenic 50.0%
GGGCTCTCCTAACAACTTCA+AGG + chr2_4:51777714-51777733 None:intergenic 50.0%
AGTTGTTAGGAGAGCCCTCA+AGG - chr2_4:51777717-51777736 Msa0333760:intron 50.0%
! CGCTAACTGGAGCATTTGCA+TGG + chr2_4:51777754-51777773 None:intergenic 50.0%
CAAGAGGTCCATGTTCCTCT+CGG - chr2_4:51778051-51778070 Msa0333760:CDS 50.0%
AAGAGGTCCATGTTCCTCTC+GGG - chr2_4:51778052-51778071 Msa0333760:CDS 50.0%
GGTCTCCAAAAGCTAGGCAA+AGG - chr2_4:51776584-51776603 Msa0333760:three_prime_UTR 50.0%
TCCGACAGAGTAATGCTACC+AGG - chr2_4:51776687-51776706 Msa0333760:three_prime_UTR 50.0%
GACAGAGTAATGCTACCAGG+AGG - chr2_4:51776690-51776709 Msa0333760:three_prime_UTR 50.0%
AGGAGCCATGTCGAAAAGAC+TGG + chr2_4:51776726-51776745 None:intergenic 50.0%
TTTCGACATGGCTCCTGATG+TGG - chr2_4:51776730-51776749 Msa0333760:three_prime_UTR 50.0%
GGGTTCATCCTCGTTGTAAC+TGG - chr2_4:51777172-51777191 Msa0333760:CDS 50.0%
GGTTGTGAGTTCTCAGAAGG+TGG + chr2_4:51777335-51777354 None:intergenic 50.0%
GGATTCACACCAATGACGCA+AGG - chr2_4:51777473-51777492 Msa0333760:intron 50.0%
GGGAAGAATCCTTGCGTCAT+TGG + chr2_4:51777485-51777504 None:intergenic 50.0%
GGCCATATGGAAAATGAGGC+AGG + chr2_4:51777518-51777537 None:intergenic 50.0%
!! TCTGAGCATTGGGGAAACAC+GGG - chr2_4:51777661-51777680 Msa0333760:intron 50.0%
AACTTCAAGGAAAGGGGGGA+AGG + chr2_4:51777701-51777720 None:intergenic 50.0%
GGGCTCTCCTAACAACTTCA+AGG + chr2_4:51777714-51777733 None:intergenic 50.0%
AGTTGTTAGGAGAGCCCTCA+AGG - chr2_4:51777717-51777736 Msa0333760:intron 50.0%
! CGCTAACTGGAGCATTTGCA+TGG + chr2_4:51777754-51777773 None:intergenic 50.0%
TAGGCAAAGGAGACTGGCGA+GGG - chr2_4:51776597-51776616 Msa0333760:three_prime_UTR 55.0%
ACTGGAACGTCTCTTCCTCC+TGG + chr2_4:51776708-51776727 None:intergenic 55.0%
CGAGAGGAACATGGACCTCT+TGG + chr2_4:51778053-51778072 None:intergenic 55.0%
TAGGCAAAGGAGACTGGCGA+GGG - chr2_4:51776597-51776616 Msa0333760:three_prime_UTR 55.0%
ACTGGAACGTCTCTTCCTCC+TGG + chr2_4:51776708-51776727 None:intergenic 55.0%
CGAGAGGAACATGGACCTCT+TGG + chr2_4:51778053-51778072 None:intergenic 55.0%
CTAGGCAAAGGAGACTGGCG+AGG - chr2_4:51776596-51776615 Msa0333760:three_prime_UTR 60.0%
CTAGGCAAAGGAGACTGGCG+AGG - chr2_4:51776596-51776615 Msa0333760:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 51776545 51778073 51776545 ID=Msa0333760;Name=Msa0333760
chr2_4 mRNA 51776545 51778073 51776545 ID=Msa0333760-mRNA-1;Parent=Msa0333760;Name=Msa0333760-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|1|0.5|1|1|0.5|2|253|245
chr2_4 exon 51776545 51777328 51776545 ID=Msa0333760-mRNA-1:exon:15494;Parent=Msa0333760-mRNA-1
chr2_4 exon 51777867 51778073 51777867 ID=Msa0333760-mRNA-1:exon:15493;Parent=Msa0333760-mRNA-1
chr2_4 CDS 51777867 51778073 51777867 ID=Msa0333760-mRNA-1:cds;Parent=Msa0333760-mRNA-1
chr2_4 CDS 51776798 51777328 51776798 ID=Msa0333760-mRNA-1:cds;Parent=Msa0333760-mRNA-1
chr2_4 three_prime_UTR 51776545 51776797 51776545 ID=Msa0333760-mRNA-1:three_prime_utr;Parent=Msa0333760-mRNA-1
Gene Sequence

>Msa0333760

GTTCCATGGACTGAGGAAGAACATAGATTGTTCTTGGTTGGTCTCCAAAAGCTAGGCAAAGGAGACTGGCGAGGGATAGCACGTAATTTTGTTGTGTCAAGGACCCCTACTCAAGTAGCAAGTCATGCACAAAAGTACTTTATCCGACAGAGTAATGCTACCAGGAGGAAGAGACGTTCCAGTCTTTTCGACATGGCTCCTGATGTGTGTCCAGATTCAACTTCGATGCCAGAAGAGCAAATACTACTTCCACCTTCTGAGAACTCACAACCTTGTAATGGAAAATCACAACCATCACTAAATCTGTCTCTCAAGTCAGAATATGAACCAATGGAAGCTACATCTGAAGAAAATGTAGAAGAGGCCAATAAGACTACAATGGGATCAAATGGATTCACACCAATGACGCAAGGATTCTTCCCACCTTATTTACCTGCCTCATTTTCCATATGGCCATCAATTGGAGCTCCCTTTGAAGAAGTCAACAAAGGAGAGACATCGTATCATCAGGTCCTCAAGCCAATACCAGTCATTCCAAAGGAACCTGTCAATGTTGATGAACTTGTGGGAATGTCTCATCTGAGCATTGGGGAAACACGGGCTCTTGATAGAGAGCCTTCCCCCCTTTCCTTGAAGTTGTTAGGAGAGCCCTCAAGGCAATCAGCATTCCATGCAAATGCTCCAGTTAGCGGTTCTGATCTAAACAGTGGCAAGAACAACGCCATTCAAGCAGTTTGA
Protein sequence

>Msa0333760

VPWTEEEHRLFLVGLQKLGKGDWRGIARNFVVSRTPTQVASHAQKYFIRQSNATRRKRRSSLFDMAPDVCPDSTSMPEEQILLPPSENSQPCNGKSQPSLNLSLKSEYEPMEATSEENVEEANKTTMGSNGFTPMTQGFFPPYLPASFSIWPSIGAPFEEVNKGETSYHQVLKPIPVIPKEPVNVDELVGMSHLSIGETRALDREPSPLSLKLLGEPSRQSAFHANAPVSGSDLNSGKNNAIQAV*