AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1443220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1443220 MtrunA17_Chr8g0377531 93.798 129 8 0 1 129 82 210 1.81e-83 244
Msa1443220 MtrunA17_Chr3g0122261 54.815 135 47 4 1 129 1 127 7.77e-42 136
Msa1443220 MtrunA17_Chr5g0434711 54.054 74 27 2 17 90 5 71 1.58e-16 70.1
Msa1443220 MtrunA17_Chr7g0224931 42.308 130 60 3 1 117 21 148 4.40e-16 71.2
Msa1443220 MtrunA17_Chr7g0224971 42.308 130 60 3 1 117 21 148 4.40e-16 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1443220 AT1G27290.2 54.762 126 48 4 1 119 1 124 8.33e-37 124
Msa1443220 AT1G27290.1 54.762 126 48 4 1 119 1 124 8.33e-37 124

Find 104 sgRNAs with CRISPR-Local

Find 177 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACAAGAAATAACTATTTC+TGG 0.134731 tig0030384:+33231 None:intergenic
GAAGCCATGCCCACAAATTA+TGG 0.284897 tig0030384:+33681 None:intergenic
TGTGGAGTTTGACATTATTA+TGG 0.287210 tig0030384:-34169 Msa1443220:three_prime_UTR
AGAGAGGGAAAAGGGTATAA+AGG 0.293809 tig0030384:+35713 None:intergenic
GATGTTATTATTGTTATAAC+AGG 0.298413 tig0030384:-33359 Msa1443220:three_prime_UTR
GGATCGGTTACTTTCGTGAT+TGG 0.305883 tig0030384:-35240 Msa1443220:CDS
CGTCTTTGAGTGGGAGGAGC+TGG 0.317023 tig0030384:-33273 Msa1443220:three_prime_UTR
ATGTTGATGCCGTGGGTTAT+TGG 0.333475 tig0030384:-35213 Msa1443220:CDS
CATATTACATGAAATAATTT+CGG 0.334699 tig0030384:+33383 None:intergenic
TGGATAATTGGTATTAGAAA+TGG 0.340616 tig0030384:+35802 None:intergenic
TTACGTGAGGAATGGATAAT+TGG 0.347004 tig0030384:+35790 None:intergenic
GATGAGAGATTGAGATAGAT+AGG 0.360209 tig0030384:+35876 None:intergenic
GGCCTAGCAAGGCTAGAATT+TGG 0.367401 tig0030384:+34090 None:intergenic
ATGAAGAGATGAGGGAAATA+TGG 0.371552 tig0030384:-33819 Msa1443220:three_prime_UTR
GTGGGTTATTGGATTGGTTA+TGG 0.372418 tig0030384:-35202 Msa1443220:CDS
CCATCTGATCCGAAATTTGT+TGG 0.374348 tig0030384:+33425 None:intergenic
CATCTCATGTCAATGCTTCA+AGG 0.387378 tig0030384:-35857 Msa1443220:intron
GCATGTCTCTAATAAGATAT+TGG 0.394270 tig0030384:-33974 Msa1443220:three_prime_UTR
ATATGTATATATGACATGTT+TGG 0.401400 tig0030384:+33497 None:intergenic
CGCTTTGTTGAATATTGAAA+TGG 0.401439 tig0030384:+35652 None:intergenic
GGCGTTGAGCAGTACGATAT+TGG 0.404711 tig0030384:-33798 Msa1443220:three_prime_UTR
AACAAGAAATAACTATTTCT+GGG 0.409264 tig0030384:+33232 None:intergenic
GGATTTGCGTCGTTTGTTCT+CGG 0.413462 tig0030384:-35288 Msa1443220:CDS
GAGAGGGAAAAGGGTATAAA+GGG 0.413563 tig0030384:+35714 None:intergenic
TGAATCAAGGACAAACTAAC+TGG 0.414033 tig0030384:+34069 None:intergenic
GATTTGCGTCGTTTGTTCTC+GGG 0.415303 tig0030384:-35287 Msa1443220:CDS
GTACTGGATCCAACAAATTT+CGG 0.418624 tig0030384:-33434 Msa1443220:three_prime_UTR
AAAATTGCAACTGTTGTTTG+TGG 0.421297 tig0030384:-34187 Msa1443220:three_prime_UTR
GGATAATTGGTATTAGAAAT+GGG 0.429748 tig0030384:+35803 None:intergenic
GTCGTTTGTTCTCGGGATTT+CGG 0.429794 tig0030384:-35280 Msa1443220:CDS
GATCGGTTACTTTCGTGATT+GGG 0.431361 tig0030384:-35239 Msa1443220:CDS
CTCGAAGAACATGGATGTAC+TGG 0.440300 tig0030384:-33450 Msa1443220:three_prime_UTR
AAGATTGAAAGAGAGGGAAA+AGG 0.443660 tig0030384:+35704 None:intergenic
TAGTTATTTCTTGTTTAGAA+AGG 0.447898 tig0030384:-33224 Msa1443220:three_prime_UTR
ATATGACATGTTTGGAAATG+AGG 0.454739 tig0030384:+33505 None:intergenic
CTTGATTCATAACCCAGATA+AGG 0.457849 tig0030384:-34055 Msa1443220:three_prime_UTR
TTTCTGGGAACAAAGTGGTT+TGG 0.463254 tig0030384:+33247 None:intergenic
AGATTGAAAGAGAGGGAAAA+GGG 0.465137 tig0030384:+35705 None:intergenic
GTTACCATAATTTGTGGGCA+TGG 0.468551 tig0030384:-33685 Msa1443220:three_prime_UTR
TATAAGTTACCATAATTTGT+GGG 0.469366 tig0030384:-33690 Msa1443220:three_prime_UTR
TTGAGACGTTCGTCGGCGGC+AGG 0.478548 tig0030384:-35327 Msa1443220:CDS
CTAGAATTTGGCTACTCTCT+AGG 0.481600 tig0030384:+34102 None:intergenic
ATATAAGTTACCATAATTTG+TGG 0.483320 tig0030384:-33691 Msa1443220:three_prime_UTR
GTCTTTGAGTGGGAGGAGCT+GGG 0.483371 tig0030384:-33272 Msa1443220:three_prime_UTR
TGCTTCAAATATGAGTTATG+TGG 0.499574 tig0030384:+33737 None:intergenic
GGCTTTGATGCTGTGTGGAT+CGG 0.501281 tig0030384:-35256 Msa1443220:CDS
ATAGCATCATTCTGTTATGT+TGG 0.502512 tig0030384:-33896 Msa1443220:three_prime_UTR
CTTATCTGGGTTATGAATCA+AGG 0.503210 tig0030384:+34056 None:intergenic
AAAGACGAAGATTGAAAGAG+AGG 0.505181 tig0030384:+35697 None:intergenic
TTGAGAATTCCATGCTTGAC+AGG 0.510579 tig0030384:-34389 Msa1443220:CDS
GATGCCGTGGGTTATTGGAT+TGG 0.512161 tig0030384:-35208 Msa1443220:CDS
CTCAACACCGACGGATTCAC+CGG 0.514030 tig0030384:+35345 None:intergenic
AAGACGAAGATTGAAAGAGA+GGG 0.514704 tig0030384:+35698 None:intergenic
GACGGATTCACCGGCACGAT+CGG 0.516430 tig0030384:+35354 None:intergenic
CAACCATGTCAAAGCTCGAC+AGG 0.517655 tig0030384:-33190 None:intergenic
CCAACAAATTTCGGATCAGA+TGG 0.520077 tig0030384:-33425 Msa1443220:three_prime_UTR
CAAAATAAACAGCAGAAATG+AGG 0.523916 tig0030384:+33945 None:intergenic
TTCTAAAGCACTAAGAATTG+AGG 0.524292 tig0030384:-33631 Msa1443220:three_prime_UTR
ATTCCATGCTTGACAGGTCT+AGG 0.525166 tig0030384:-34383 Msa1443220:intron
GATCGTGCCGGTGAATCCGT+CGG 0.526152 tig0030384:-35352 Msa1443220:CDS
TTGATTCATAACCCAGATAA+GGG 0.528184 tig0030384:-34054 Msa1443220:three_prime_UTR
TTTGACAAGAGGGGTTTGCT+TGG 0.532173 tig0030384:+35745 None:intergenic
TGGCGAAGCAAAGCAAAGAA+TGG 0.536106 tig0030384:+35131 None:intergenic
GTTCTATAGAGTGCATGAGC+AGG 0.538940 tig0030384:+33768 None:intergenic
CACATTGCCACGTCTTTGAG+TGG 0.543568 tig0030384:-33283 Msa1443220:three_prime_UTR
AAACTGGGTTACGTGAGGAA+TGG 0.550237 tig0030384:+35782 None:intergenic
GAAATTGTTATGAAGAGATG+AGG 0.551132 tig0030384:-33828 Msa1443220:three_prime_UTR
GTCGGTGTTGAGACGTTCGT+CGG 0.552778 tig0030384:-35334 Msa1443220:CDS
CGGCACGATCGGAGATCGAT+CGG 0.564859 tig0030384:+35365 None:intergenic
ACGCCTGTCGAGCTTTGACA+TGG 0.570004 tig0030384:+33187 None:intergenic
ACATTGCCACGTCTTTGAGT+GGG 0.581057 tig0030384:-33282 Msa1443220:three_prime_UTR
ACATAACTCATATTTGAAGC+AGG 0.582255 tig0030384:-33735 Msa1443220:three_prime_UTR
CGATCGATCTCCGATCGTGC+CGG 0.583698 tig0030384:-35364 Msa1443220:CDS
TGTTATAACAGGTATTGATG+TGG 0.584670 tig0030384:-33348 Msa1443220:three_prime_UTR
GTACCTAGACCTGTCAAGCA+TGG 0.588585 tig0030384:+34380 None:intergenic
TGGGTTTATGTTGATGCCGT+GGG 0.591957 tig0030384:-35220 Msa1443220:CDS
CATAACCCAGATAAGGGTCA+AGG 0.592729 tig0030384:-34048 Msa1443220:three_prime_UTR
TGGTGAACTGATTGATGCAT+GGG 0.593183 tig0030384:-34149 Msa1443220:three_prime_UTR
AGACATGCTTCAATGAGTTG+AGG 0.594995 tig0030384:+33989 None:intergenic
GATCGATCGGAATAAGATAA+CGG 0.597785 tig0030384:+35378 None:intergenic
AACTATTTCTGGGAACAAAG+TGG 0.598692 tig0030384:+33242 None:intergenic
TTGGGTTTATGTTGATGCCG+TGG 0.602091 tig0030384:-35221 Msa1443220:CDS
ATGGTGAACTGATTGATGCA+TGG 0.603148 tig0030384:-34150 Msa1443220:three_prime_UTR
ACAAACTAACTGGCCTAGCA+AGG 0.603397 tig0030384:+34079 None:intergenic
AGCCAAATTCTAGCCTTGCT+AGG 0.604360 tig0030384:-34092 Msa1443220:three_prime_UTR
CATATTTGAAGCAGGAATCG+TGG 0.613855 tig0030384:-33727 Msa1443220:three_prime_UTR
TTGCCACGTCTTTGAGTGGG+AGG 0.614025 tig0030384:-33279 Msa1443220:three_prime_UTR
GTTTGTTCTCGGGATTTCGG+TGG 0.621102 tig0030384:-35277 Msa1443220:CDS
GCAGGTCAGATAACTCCGGC+GGG 0.623444 tig0030384:-35309 Msa1443220:CDS
AAATTGTTATGAAGAGATGA+GGG 0.626191 tig0030384:-33827 Msa1443220:three_prime_UTR
GACGAACGTCTCAACACCGA+CGG 0.630029 tig0030384:+35336 None:intergenic
GGTGTTGAGACGTTCGTCGG+CGG 0.630502 tig0030384:-35331 Msa1443220:CDS
TCGCCACGTAAAGATTCTCC+CGG 0.632756 tig0030384:-35114 Msa1443220:intron
TCGGTGGCTTTGATGCTGTG+TGG 0.645493 tig0030384:-35261 Msa1443220:CDS
GGCAGGTCAGATAACTCCGG+CGG 0.647696 tig0030384:-35310 Msa1443220:CDS
GGCGGCAGGTCAGATAACTC+CGG 0.648034 tig0030384:-35313 Msa1443220:CDS
TCGGAATAAGATAACGGCGT+CGG 0.648078 tig0030384:+35384 None:intergenic
CACTCAAAGACGTGGCAATG+TGG 0.656986 tig0030384:+33284 None:intergenic
TCATATATACATATATAGAG+AGG 0.657329 tig0030384:-33488 Msa1443220:three_prime_UTR
ACAAACGACGCAAATCCCGC+CGG 0.670719 tig0030384:+35294 None:intergenic
ATAACCAATCCAATAACCCA+CGG 0.672738 tig0030384:+35204 None:intergenic
ATGATTTGTCTCGAAGAACA+TGG 0.674713 tig0030384:-33459 Msa1443220:three_prime_UTR
TTACCGGGAGAATCTTTACG+TGG 0.685734 tig0030384:+35111 None:intergenic
GCTCCTCCCACTCAAAGACG+TGG 0.694399 tig0030384:+33276 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGAGATAAATTTATATGTA+TGG - tig0030384:34130-34149 Msa1443220:three_prime_UTR 15.0%
!!! GTTTATCTTCTTTAAAATTA+AGG - tig0030384:34401-34420 Msa1443220:CDS 15.0%
!! CATATTACATGAAATAATTT+CGG + tig0030384:35686-35705 None:intergenic 15.0%
!!! TGCTTTTTTAGAATTTTATA+TGG - tig0030384:35751-35770 Msa1443220:five_prime_UTR 15.0%
!!! TTTTTAGAATTTTATATGGT+AGG - tig0030384:35755-35774 Msa1443220:five_prime_UTR 15.0%
!! GTTAATGAAAAAGAAAAACT+GGG + tig0030384:33302-33321 None:intergenic 20.0%
!! TTATGTTTCTTAGTTAGTTA+TGG - tig0030384:34067-34086 Msa1443220:three_prime_UTR 20.0%
!! TATGTTTCTTAGTTAGTTAT+GGG - tig0030384:34068-34087 Msa1443220:three_prime_UTR 20.0%
!! AAGAAGATAAACATGTATAT+CGG + tig0030384:34393-34412 None:intergenic 20.0%
!!! ATCTTCTTTAAAATTAAGGT+TGG - tig0030384:34405-34424 Msa1443220:CDS 20.0%
!!! ATTGTAGCTTTCATTTTAAA+AGG - tig0030384:34641-34660 Msa1443220:intron 20.0%
!!! TTGTAGCTTTCATTTTAAAA+GGG - tig0030384:34642-34661 Msa1443220:intron 20.0%
!!! TTTTAAAAGGGATATTCATA+TGG - tig0030384:34654-34673 Msa1443220:intron 20.0%
!!! CATACAACAAATTAGTTTTT+TGG + tig0030384:34788-34807 None:intergenic 20.0%
!! ATATAAGTTACCATAATTTG+TGG - tig0030384:35375-35394 Msa1443220:CDS 20.0%
!!! TATAAGTTACCATAATTTGT+GGG - tig0030384:35376-35395 Msa1443220:CDS 20.0%
!! AACAGATATCAATAAAACAA+AGG + tig0030384:35494-35513 None:intergenic 20.0%
!! ATCTGTAAATTGCTAATTAT+AGG - tig0030384:35519-35538 Msa1443220:five_prime_UTR 20.0%
!! ATATGTATATATGACATGTT+TGG + tig0030384:35572-35591 None:intergenic 20.0%
!! TCATATATACATATATAGAG+AGG - tig0030384:35578-35597 Msa1443220:intron 20.0%
!! GATGTTATTATTGTTATAAC+AGG - tig0030384:35707-35726 Msa1443220:five_prime_UTR 20.0%
!!! AACAAGAAATAACTATTTCT+GGG + tig0030384:35837-35856 None:intergenic 20.0%
!! AAACAAGAAATAACTATTTC+TGG + tig0030384:35838-35857 None:intergenic 20.0%
!!! TAGTTATTTCTTGTTTAGAA+AGG - tig0030384:35842-35861 Msa1443220:intron 20.0%
! GGATAATTGGTATTAGAAAT+GGG + tig0030384:33266-33285 None:intergenic 25.0%
! TGGATAATTGGTATTAGAAA+TGG + tig0030384:33267-33286 None:intergenic 25.0%
! GGTTAATGAAAAAGAAAAAC+TGG + tig0030384:33303-33322 None:intergenic 25.0%
! AAGAAGAAAGAAGAAGAAAT+TGG + tig0030384:33463-33482 None:intergenic 25.0%
!! CTCATCATTTTCTTGATTTT+TGG + tig0030384:33580-33599 None:intergenic 25.0%
!! TGTTTCTTTTCTTTCTGTTT+TGG - tig0030384:33906-33925 Msa1443220:three_prime_UTR 25.0%
!! GTTTCTTTTCTTTCTGTTTT+GGG - tig0030384:33907-33926 Msa1443220:three_prime_UTR 25.0%
! ATAGATTATGATTTCTTACC+GGG + tig0030384:33973-33992 None:intergenic 25.0%
! CATAGATTATGATTTCTTAC+CGG + tig0030384:33974-33993 None:intergenic 25.0%
! ATGTTTCTTAGTTAGTTATG+GGG - tig0030384:34069-34088 Msa1443220:three_prime_UTR 25.0%
!! ATTTAAAACGATAACACACA+GGG + tig0030384:34223-34242 None:intergenic 25.0%
!! AATTTAAAACGATAACACAC+AGG + tig0030384:34224-34243 None:intergenic 25.0%
! CAGATAATTGTAGTTAATTG+CGG - tig0030384:34271-34290 Msa1443220:intron 25.0%
!! GAATTTTTCTGTAATCAGAA+AGG - tig0030384:34548-34567 Msa1443220:intron 25.0%
!! TTTTATCCGTTCATGATTTT+AGG - tig0030384:34616-34635 Msa1443220:intron 25.0%
! CAATAACCTAAAATCATGAA+CGG + tig0030384:34625-34644 None:intergenic 25.0%
! GTCTAGGTACATATTATATT+GGG - tig0030384:34699-34718 Msa1443220:intron 25.0%
! ATATTATATTGGGAGTAAGT+GGG - tig0030384:34709-34728 Msa1443220:intron 25.0%
!!! TAGTTTTTTGGTATTCATCA+AGG + tig0030384:34776-34795 None:intergenic 25.0%
!!! TTTATTTTGACTTGAATGTG+TGG - tig0030384:35135-35154 Msa1443220:CDS 25.0%
! AAATTGTTATGAAGAGATGA+GGG - tig0030384:35239-35258 Msa1443220:CDS 25.0%
! CGCTTTGTTGAATATTGAAA+TGG + tig0030384:33417-33436 None:intergenic 30.0%
AAGAAGAAGAAATTGGATAG+AGG + tig0030384:33456-33475 None:intergenic 30.0%
!! CATAATCTATGTTGCTTTCT+TGG - tig0030384:33983-34002 Msa1443220:three_prime_UTR 30.0%
TTTCTTGGTTCAAATATTGC+TGG - tig0030384:33998-34017 Msa1443220:three_prime_UTR 30.0%
! CAAATATTGCTGGTTAGAAT+TGG - tig0030384:34008-34027 Msa1443220:three_prime_UTR 30.0%
ACATTTAAGAGTAGTAGTAG+TGG - tig0030384:34170-34189 Msa1443220:three_prime_UTR 30.0%
TGAAGAGACATCAAATACAT+AGG - tig0030384:34315-34334 Msa1443220:intron 30.0%
AGACATCAAATACATAGGAT+GGG - tig0030384:34320-34339 Msa1443220:intron 30.0%
AAATTATACACTTTCTGTGG+TGG + tig0030384:34475-34494 None:intergenic 30.0%
TGCAAATTATACACTTTCTG+TGG + tig0030384:34478-34497 None:intergenic 30.0%
GGTCTAGGTACATATTATAT+TGG - tig0030384:34698-34717 Msa1443220:intron 30.0%
CATATTATATTGGGAGTAAG+TGG - tig0030384:34708-34727 Msa1443220:intron 30.0%
TATTATATTGGGAGTAAGTG+GGG - tig0030384:34710-34729 Msa1443220:intron 30.0%
!!! TGCTTTTTTCAGTCAAAGAT+TGG - tig0030384:34806-34825 Msa1443220:intron 30.0%
!!! GCTTTTTTCAGTCAAAGATT+GGG - tig0030384:34807-34826 Msa1443220:intron 30.0%
! AAAATTGCAACTGTTGTTTG+TGG - tig0030384:34879-34898 Msa1443220:intron 30.0%
TGTGGAGTTTGACATTATTA+TGG - tig0030384:34897-34916 Msa1443220:intron 30.0%
TTGATTCATAACCCAGATAA+GGG - tig0030384:35012-35031 Msa1443220:intron 30.0%
GCATGTCTCTAATAAGATAT+TGG - tig0030384:35092-35111 Msa1443220:intron 30.0%
CAAAATAAACAGCAGAAATG+AGG + tig0030384:35124-35143 None:intergenic 30.0%
ATAGCATCATTCTGTTATGT+TGG - tig0030384:35170-35189 Msa1443220:CDS 30.0%
AAAAATCATGTAATGGTGTC+AGG + tig0030384:35204-35223 None:intergenic 30.0%
CAACAGCAAAAATCATGTAA+TGG + tig0030384:35211-35230 None:intergenic 30.0%
GAAATTGTTATGAAGAGATG+AGG - tig0030384:35238-35257 Msa1443220:CDS 30.0%
TGCTTCAAATATGAGTTATG+TGG + tig0030384:35332-35351 None:intergenic 30.0%
! ACATAACTCATATTTGAAGC+AGG - tig0030384:35331-35350 Msa1443220:CDS 30.0%
!! TTCTAAAGCACTAAGAATTG+AGG - tig0030384:35435-35454 Msa1443220:CDS 30.0%
TGTTTGGAAATGAGGAAAAA+AGG + tig0030384:35556-35575 None:intergenic 30.0%
ATATGACATGTTTGGAAATG+AGG + tig0030384:35564-35583 None:intergenic 30.0%
!! TGTTATAACAGGTATTGATG+TGG - tig0030384:35718-35737 Msa1443220:five_prime_UTR 30.0%
TTACGTGAGGAATGGATAAT+TGG + tig0030384:33279-33298 None:intergenic 35.0%
!! GAAAAAGTGTTTGACAAGAG+GGG + tig0030384:33333-33352 None:intergenic 35.0%
!! GGAAAAAGTGTTTGACAAGA+GGG + tig0030384:33334-33353 None:intergenic 35.0%
AGATTGAAAGAGAGGGAAAA+GGG + tig0030384:33364-33383 None:intergenic 35.0%
AAGATTGAAAGAGAGGGAAA+AGG + tig0030384:33365-33384 None:intergenic 35.0%
AAGACGAAGATTGAAAGAGA+GGG + tig0030384:33371-33390 None:intergenic 35.0%
AAAGACGAAGATTGAAAGAG+AGG + tig0030384:33372-33391 None:intergenic 35.0%
GATCGATCGGAATAAGATAA+CGG + tig0030384:33691-33710 None:intergenic 35.0%
ATAACCAATCCAATAACCCA+CGG + tig0030384:33865-33884 None:intergenic 35.0%
! GTTATGGTGTTTTATGTTGC+TGG - tig0030384:33880-33899 Msa1443220:three_prime_UTR 35.0%
CTGCATTAACAATGATCACT+TGG + tig0030384:34254-34273 None:intergenic 35.0%
ATGTCTCTTCAACCACATTA+CGG + tig0030384:34306-34325 None:intergenic 35.0%
GAGACATCAAATACATAGGA+TGG - tig0030384:34319-34338 Msa1443220:intron 35.0%
! TAATTTGCAACACCCATATC+AGG - tig0030384:34489-34508 Msa1443220:intron 35.0%
AATTTGCAACACCCATATCA+GGG - tig0030384:34490-34509 Msa1443220:intron 35.0%
ATCTAAGCTATCCCTGATAT+GGG + tig0030384:34504-34523 None:intergenic 35.0%
TATCTAAGCTATCCCTGATA+TGG + tig0030384:34505-34524 None:intergenic 35.0%
AGTAAGTGGGGTTAAGTTTA+TGG - tig0030384:34722-34741 Msa1443220:intron 35.0%
GTAAGTGGGGTTAAGTTTAT+GGG - tig0030384:34723-34742 Msa1443220:intron 35.0%
TGAATCAAGGACAAACTAAC+TGG + tig0030384:35000-35019 None:intergenic 35.0%
CTTATCTGGGTTATGAATCA+AGG + tig0030384:35013-35032 None:intergenic 35.0%
CTTGATTCATAACCCAGATA+AGG - tig0030384:35011-35030 Msa1443220:intron 35.0%
! TTTTACCTTGACCCTTATCT+GGG + tig0030384:35026-35045 None:intergenic 35.0%
ATGAAGAGATGAGGGAAATA+TGG - tig0030384:35247-35266 Msa1443220:CDS 35.0%
GATAACAAAAGAAAGAAGCC+AGG + tig0030384:35472-35491 None:intergenic 35.0%
! ATGATTTGTCTCGAAGAACA+TGG - tig0030384:35607-35626 Msa1443220:intron 35.0%
GTACTGGATCCAACAAATTT+CGG - tig0030384:35632-35651 Msa1443220:intron 35.0%
!! AACTATTTCTGGGAACAAAG+TGG + tig0030384:35827-35846 None:intergenic 35.0%
CATCTCATGTCAATGCTTCA+AGG - tig0030384:33209-33228 Msa1443220:three_prime_UTR 40.0%
AAGAAAAACTGGGTTACGTG+AGG + tig0030384:33292-33311 None:intergenic 40.0%
!! GGGAAAAAGTGTTTGACAAG+AGG + tig0030384:33335-33354 None:intergenic 40.0%
! GAGAGGGAAAAGGGTATAAA+GGG + tig0030384:33355-33374 None:intergenic 40.0%
! AGAGAGGGAAAAGGGTATAA+AGG + tig0030384:33356-33375 None:intergenic 40.0%
! GATCGGTTACTTTCGTGATT+GGG - tig0030384:33827-33846 Msa1443220:three_prime_UTR 40.0%
!! GTGGGTTATTGGATTGGTTA+TGG - tig0030384:33864-33883 Msa1443220:three_prime_UTR 40.0%
TTGTAGTTAATTGCGGCTGA+TGG - tig0030384:34278-34297 Msa1443220:intron 40.0%
TGTAGTTAATTGCGGCTGAT+GGG - tig0030384:34279-34298 Msa1443220:intron 40.0%
TTGAGAATTCCATGCTTGAC+AGG - tig0030384:34677-34696 Msa1443220:intron 40.0%
ATGGTGAACTGATTGATGCA+TGG - tig0030384:34916-34935 Msa1443220:intron 40.0%
TGGTGAACTGATTGATGCAT+GGG - tig0030384:34917-34936 Msa1443220:intron 40.0%
CTAGAATTTGGCTACTCTCT+AGG + tig0030384:34967-34986 None:intergenic 40.0%
! CTTTTACCTTGACCCTTATC+TGG + tig0030384:35027-35046 None:intergenic 40.0%
AGACATGCTTCAATGAGTTG+AGG + tig0030384:35080-35099 None:intergenic 40.0%
!!! TGATTTTTGCTGTTGCAGAG+TGG - tig0030384:35216-35235 Msa1443220:CDS 40.0%
! CATATTTGAAGCAGGAATCG+TGG - tig0030384:35339-35358 Msa1443220:CDS 40.0%
! GTTACCATAATTTGTGGGCA+TGG - tig0030384:35381-35400 Msa1443220:CDS 40.0%
AACAAAAGAAAGAAGCCAGG+CGG + tig0030384:35469-35488 None:intergenic 40.0%
CCATCTGATCCGAAATTTGT+TGG + tig0030384:35644-35663 None:intergenic 40.0%
CCAACAAATTTCGGATCAGA+TGG - tig0030384:35641-35660 Msa1443220:intron 40.0%
! TTTCTGGGAACAAAGTGGTT+TGG + tig0030384:35822-35841 None:intergenic 40.0%
AAACTGGGTTACGTGAGGAA+TGG + tig0030384:33287-33306 None:intergenic 45.0%
! TTTGACAAGAGGGGTTTGCT+TGG + tig0030384:33324-33343 None:intergenic 45.0%
! TCGGAATAAGATAACGGCGT+CGG + tig0030384:33685-33704 None:intergenic 45.0%
GGATTTGCGTCGTTTGTTCT+CGG - tig0030384:33778-33797 Msa1443220:three_prime_UTR 45.0%
GATTTGCGTCGTTTGTTCTC+GGG - tig0030384:33779-33798 Msa1443220:three_prime_UTR 45.0%
GTCGTTTGTTCTCGGGATTT+CGG - tig0030384:33786-33805 Msa1443220:three_prime_UTR 45.0%
! GGATCGGTTACTTTCGTGAT+TGG - tig0030384:33826-33845 Msa1443220:three_prime_UTR 45.0%
TTGGGTTTATGTTGATGCCG+TGG - tig0030384:33845-33864 Msa1443220:three_prime_UTR 45.0%
TGGGTTTATGTTGATGCCGT+GGG - tig0030384:33846-33865 Msa1443220:three_prime_UTR 45.0%
! ATGTTGATGCCGTGGGTTAT+TGG - tig0030384:33853-33872 Msa1443220:three_prime_UTR 45.0%
TGGCGAAGCAAAGCAAAGAA+TGG + tig0030384:33938-33957 None:intergenic 45.0%
TTACCGGGAGAATCTTTACG+TGG + tig0030384:33958-33977 None:intergenic 45.0%
GTAGTTAATTGCGGCTGATG+GGG - tig0030384:34280-34299 Msa1443220:intron 45.0%
ATTCCATGCTTGACAGGTCT+AGG - tig0030384:34683-34702 Msa1443220:intron 45.0%
!! AGCCAAATTCTAGCCTTGCT+AGG - tig0030384:34974-34993 Msa1443220:intron 45.0%
! ACAAACTAACTGGCCTAGCA+AGG + tig0030384:34990-35009 None:intergenic 45.0%
CATAACCCAGATAAGGGTCA+AGG - tig0030384:35018-35037 Msa1443220:intron 45.0%
GTTCTATAGAGTGCATGAGC+AGG + tig0030384:35301-35320 None:intergenic 45.0%
GAAGCCATGCCCACAAATTA+TGG + tig0030384:35388-35407 None:intergenic 45.0%
ACAAAAGAAAGAAGCCAGGC+GGG + tig0030384:35468-35487 None:intergenic 45.0%
CTCGAAGAACATGGATGTAC+TGG - tig0030384:35616-35635 Msa1443220:intron 45.0%
ACATTGCCACGTCTTTGAGT+GGG - tig0030384:35784-35803 Msa1443220:five_prime_UTR 45.0%
! TGGTTGACGACGACGTTTTG+AGG + tig0030384:33397-33416 None:intergenic 50.0%
GTTTGTTCTCGGGATTTCGG+TGG - tig0030384:33789-33808 Msa1443220:three_prime_UTR 50.0%
! GGCTTTGATGCTGTGTGGAT+CGG - tig0030384:33810-33829 Msa1443220:three_prime_UTR 50.0%
! GATGCCGTGGGTTATTGGAT+TGG - tig0030384:33858-33877 Msa1443220:three_prime_UTR 50.0%
TCGCCACGTAAAGATTCTCC+CGG - tig0030384:33952-33971 Msa1443220:three_prime_UTR 50.0%
GTACCTAGACCTGTCAAGCA+TGG + tig0030384:34689-34708 None:intergenic 50.0%
GGCCTAGCAAGGCTAGAATT+TGG + tig0030384:34979-34998 None:intergenic 50.0%
! GGCGTTGAGCAGTACGATAT+TGG - tig0030384:35268-35287 Msa1443220:CDS 50.0%
CACTCAAAGACGTGGCAATG+TGG + tig0030384:35785-35804 None:intergenic 50.0%
CACATTGCCACGTCTTTGAG+TGG - tig0030384:35783-35802 Msa1443220:five_prime_UTR 50.0%
! CTCAACACCGACGGATTCAC+CGG + tig0030384:33724-33743 None:intergenic 55.0%
GACGAACGTCTCAACACCGA+CGG + tig0030384:33733-33752 None:intergenic 55.0%
!! GTCGGTGTTGAGACGTTCGT+CGG - tig0030384:33732-33751 Msa1443220:three_prime_UTR 55.0%
ACAAACGACGCAAATCCCGC+CGG + tig0030384:33775-33794 None:intergenic 55.0%
!! TCGGTGGCTTTGATGCTGTG+TGG - tig0030384:33805-33824 Msa1443220:three_prime_UTR 55.0%
TTGCCACGTCTTTGAGTGGG+AGG - tig0030384:35787-35806 Msa1443220:five_prime_UTR 55.0%
GTCTTTGAGTGGGAGGAGCT+GGG - tig0030384:35794-35813 Msa1443220:five_prime_UTR 55.0%
CGGCACGATCGGAGATCGAT+CGG + tig0030384:33704-33723 None:intergenic 60.0%
CGATCGATCTCCGATCGTGC+CGG - tig0030384:33702-33721 Msa1443220:three_prime_UTR 60.0%
! GACGGATTCACCGGCACGAT+CGG + tig0030384:33715-33734 None:intergenic 60.0%
! GATCGTGCCGGTGAATCCGT+CGG - tig0030384:33714-33733 Msa1443220:three_prime_UTR 60.0%
! GGTGTTGAGACGTTCGTCGG+CGG - tig0030384:33735-33754 Msa1443220:three_prime_UTR 60.0%
GGCGGCAGGTCAGATAACTC+CGG - tig0030384:33753-33772 Msa1443220:three_prime_UTR 60.0%
GGCAGGTCAGATAACTCCGG+CGG - tig0030384:33756-33775 Msa1443220:three_prime_UTR 60.0%
GCAGGTCAGATAACTCCGGC+GGG - tig0030384:33757-33776 Msa1443220:three_prime_UTR 60.0%
ATTGAGGAGTCGTTCCCGCC+TGG - tig0030384:35451-35470 Msa1443220:CDS 60.0%
GCTCCTCCCACTCAAAGACG+TGG + tig0030384:35793-35812 None:intergenic 60.0%
CGTCTTTGAGTGGGAGGAGC+TGG - tig0030384:35793-35812 Msa1443220:five_prime_UTR 60.0%
TTGAGACGTTCGTCGGCGGC+AGG - tig0030384:33739-33758 Msa1443220:three_prime_UTR 65.0%
CGGCTGATGGGGCCGTAATG+TGG - tig0030384:34291-34310 Msa1443220:intron 65.0%
Chromosome Type Strat End Strand Name
tig0030384 gene 33193 35895 33193 ID=Msa1443220;Name=Msa1443220
tig0030384 mRNA 33193 35895 33193 ID=Msa1443220-mRNA-1;Parent=Msa1443220;Name=Msa1443220-mRNA-1;_AED=0.12;_eAED=0.12;_QI=283|0.6|0.83|0.83|0.4|0.16|6|971|157
tig0030384 exon 33193 33547 33193 ID=Msa1443220-mRNA-1:exon:13797;Parent=Msa1443220-mRNA-1
tig0030384 exon 33630 34270 33630 ID=Msa1443220-mRNA-1:exon:13796;Parent=Msa1443220-mRNA-1
tig0030384 exon 34384 34450 34384 ID=Msa1443220-mRNA-1:exon:13795;Parent=Msa1443220-mRNA-1
tig0030384 exon 35115 35551 35115 ID=Msa1443220-mRNA-1:exon:13794;Parent=Msa1443220-mRNA-1
tig0030384 exon 35645 35834 35645 ID=Msa1443220-mRNA-1:exon:13793;Parent=Msa1443220-mRNA-1
tig0030384 exon 35858 35895 35858 ID=Msa1443220-mRNA-1:exon:13792;Parent=Msa1443220-mRNA-1
tig0030384 five_prime_UTR 35858 35895 35858 ID=Msa1443220-mRNA-1:five_prime_utr;Parent=Msa1443220-mRNA-1
tig0030384 five_prime_UTR 35645 35834 35645 ID=Msa1443220-mRNA-1:five_prime_utr;Parent=Msa1443220-mRNA-1
tig0030384 five_prime_UTR 35497 35551 35497 ID=Msa1443220-mRNA-1:five_prime_utr;Parent=Msa1443220-mRNA-1
tig0030384 CDS 35115 35496 35115 ID=Msa1443220-mRNA-1:cds;Parent=Msa1443220-mRNA-1
tig0030384 CDS 34384 34450 34384 ID=Msa1443220-mRNA-1:cds;Parent=Msa1443220-mRNA-1
tig0030384 CDS 34246 34270 34246 ID=Msa1443220-mRNA-1:cds;Parent=Msa1443220-mRNA-1
tig0030384 three_prime_UTR 33630 34245 33630 ID=Msa1443220-mRNA-1:three_prime_utr;Parent=Msa1443220-mRNA-1
tig0030384 three_prime_UTR 33193 33547 33193 ID=Msa1443220-mRNA-1:three_prime_utr;Parent=Msa1443220-mRNA-1
Gene Sequence

>Msa1443220

ATGATGAGAAGAAATCAAGATCAAGAACAACAATCAAAGATGATTTGCGAATTATCATCTCTACTTTTCAATCTTCTTCTACAATCTTCACCGACGCCGTTATCTTATTCCGATCGATCTCCGATCGTGCCGGTGAATCCGTCGGTGTTGAGACGTTCGTCGGCGGCAGGTCAGATAACTCCGGCGGGATTTGCGTCGTTTGTTCTCGGGATTTCGGTGGCTTTGATGCTGTGTGGATCGGTTACTTTCGTGATTGGGTTTATGTTGATGCCGTGGGTTATTGGATTGGTTATGGTGTTTTATGTTGCTGGTGTTGTTTCTTTTCTTTCTGTTTTGGGTCGCTCCATTCTTTGCTTTGCTTCGCCACGTAAAGATTCTCCCGGTTATTGTAGCTTTCATTTTAAAAGGGATATTCATATGGTTGAGAATTCCATGCTTGACAGGTCTAGTCAAAGATTGGGTCTGCAATATTAG
Protein sequence

>Msa1443220

MMRRNQDQEQQSKMICELSSLLFNLLLQSSPTPLSYSDRSPIVPVNPSVLRRSSAAGQITPAGFASFVLGISVALMLCGSVTFVIGFMLMPWVIGLVMVFYVAGVVSFLSVLGRSILCFASPRKDSPGYCSFHFKRDIHMVENSMLDRSSQRLGLQY*