AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0337680

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0337680 MtrunA17_Chr2g0325131 96.023 352 6 2 1 352 1 344 0.0 682
Msa0337680 MtrunA17_Chr4g0040891 62.824 347 92 14 1 330 1 327 2.41e-124 360
Msa0337680 MtrunA17_Chr2g0316551 53.870 323 116 10 1 302 1 311 3.03e-96 290
Msa0337680 MtrunA17_Chr8g0380611 35.094 265 145 8 44 294 73 324 4.45e-28 112
Msa0337680 MtrunA17_Chr5g0432701 48.936 94 48 0 198 291 158 251 2.58e-20 90.9
Msa0337680 MtrunA17_Chr4g0036071 48.454 97 50 0 197 293 358 454 4.93e-20 91.3
Msa0337680 MtrunA17_Chr2g0329551 44.737 114 58 2 197 310 219 327 3.18e-19 88.2
Msa0337680 MtrunA17_Chr3g0107491 46.237 93 50 0 199 291 177 269 1.63e-18 85.9
Msa0337680 MtrunA17_Chr6g0463571 46.000 100 54 0 197 296 149 248 6.30e-18 83.2
Msa0337680 MtrunA17_Chr3g0128781 45.745 94 51 0 198 291 107 200 3.34e-17 81.3
Msa0337680 MtrunA17_Chr1g0156051 48.980 98 46 2 198 293 386 481 1.40e-12 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0337680 AT3G58120.1 59.130 345 109 10 1 330 1 328 3.36e-120 350
Msa0337680 AT2G42380.2 57.101 345 107 8 1 330 1 319 5.09e-114 334
Msa0337680 AT2G42380.1 53.623 345 108 9 1 330 1 308 1.65e-102 304
Msa0337680 AT3G58120.2 58.020 293 96 8 1 278 1 281 5.38e-100 297
Msa0337680 AT2G42380.5 56.701 291 87 7 1 278 1 265 8.32e-93 278
Msa0337680 AT2G42380.3 49.206 252 89 7 1 239 1 226 1.40e-61 197
Msa0337680 AT2G42380.4 49.206 252 89 7 1 239 1 226 1.40e-61 197
Msa0337680 AT1G58110.1 30.448 335 161 11 2 294 20 324 2.40e-27 111
Msa0337680 AT1G58110.2 30.448 335 161 11 2 294 20 324 2.40e-27 111
Msa0337680 AT1G43700.1 46.491 114 56 1 185 293 175 288 7.34e-21 92.4
Msa0337680 AT4G38900.2 48.421 95 49 0 197 291 392 486 7.00e-20 91.3
Msa0337680 AT4G38900.3 48.421 95 49 0 197 291 392 486 7.00e-20 91.3
Msa0337680 AT5G04840.1 29.276 304 163 8 2 296 5 265 3.01e-19 87.4
Msa0337680 AT2G40620.1 46.316 95 51 0 197 291 146 240 4.58e-19 87.8
Msa0337680 AT1G06850.1 48.387 93 48 0 199 291 148 240 1.61e-18 85.9
Msa0337680 AT1G06070.1 46.316 95 51 0 197 291 208 302 1.70e-18 86.7
Msa0337680 AT2G21230.4 36.486 148 89 2 197 340 368 514 3.19e-18 86.3
Msa0337680 AT2G21230.1 36.486 148 89 2 197 340 368 514 3.19e-18 86.3
Msa0337680 AT2G31370.5 31.174 247 143 5 62 291 57 293 7.04e-18 84.3
Msa0337680 AT2G31370.2 31.174 247 143 5 62 291 57 293 7.54e-18 84.3
Msa0337680 AT2G31370.4 31.174 247 143 5 62 291 57 293 7.54e-18 84.3
Msa0337680 AT2G31370.1 31.174 247 143 5 62 291 57 293 7.54e-18 84.3
Msa0337680 AT2G31370.7 31.174 247 143 5 62 291 57 293 7.54e-18 84.3
Msa0337680 AT2G31370.3 31.174 247 143 5 62 291 57 293 7.54e-18 84.3
Msa0337680 AT2G31370.6 31.174 247 143 5 62 291 57 293 7.54e-18 84.3
Msa0337680 AT4G38900.1 45.545 101 49 1 197 291 392 492 1.09e-17 84.7
Msa0337680 AT1G35490.2 28.226 248 133 7 46 291 54 258 7.98e-17 80.5
Msa0337680 AT1G35490.1 28.226 248 133 7 46 291 54 258 7.98e-17 80.5
Msa0337680 AT5G04840.2 29.371 286 150 8 2 278 5 247 5.23e-16 77.0
Msa0337680 AT2G21230.3 34.416 154 90 3 197 340 368 520 6.75e-16 79.3
Msa0337680 AT2G21230.2 46.341 82 44 0 197 278 368 449 3.89e-14 73.6
Msa0337680 AT5G07160.1 41.237 97 57 0 195 291 44 140 2.92e-12 64.3
Msa0337680 AT1G06850.2 68.889 45 14 0 198 242 147 191 5.89e-11 62.4

Find 77 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACATTCCCTTGTGTGGTTTC+TGG 0.097414 2_4:-57772468 Msa0337680:five_prime_UTR
GAAATATACATATTTATTAA+AGG 0.205907 2_4:-57772446 Msa0337680:five_prime_UTR
GGTGACATGTTTGGGATCTT+AGG 0.259620 2_4:+57772210 None:intergenic
GTTTGTAGTGACAATGTTAT+TGG 0.263094 2_4:+57772131 None:intergenic
TTTGAGGTCTTGGATTTAAT+TGG 0.274873 2_4:-57768638 Msa0337680:three_prime_UTR
GCATTAGAATCTTGGCAAAT+AGG 0.275046 2_4:-57771220 Msa0337680:intron
CCTCAAATTTGTTGTTGTTA+AGG 0.275676 2_4:+57772295 None:intergenic
GAGAAGAAGGAAAATGATTT+AGG 0.296863 2_4:-57771787 Msa0337680:CDS
GCCAACTTGGTGACATGTTT+GGG 0.333815 2_4:+57772202 None:intergenic
TTATTAAAGGGTTGTGTTAG+AGG 0.345247 2_4:-57772433 Msa0337680:five_prime_UTR
CATTGGCTTTCTACCTCATC+AGG 0.351950 2_4:+57771721 None:intergenic
AAATATACATATTTATTAAA+GGG 0.356758 2_4:-57772445 Msa0337680:five_prime_UTR
AAATTGCGCTGTATATATAA+TGG 0.357460 2_4:-57768530 Msa0337680:three_prime_UTR
GGTCTTGGATTTAATTGGTT+TGG 0.375520 2_4:-57768633 Msa0337680:three_prime_UTR
GCGTAGTGTAACTTCATTGC+AGG 0.377738 2_4:-57771144 Msa0337680:intron
GGCCAACTTGGTGACATGTT+TGG 0.381627 2_4:+57772201 None:intergenic
GACAATGTTATTGGTTGAAA+TGG 0.385822 2_4:+57772140 None:intergenic
TCTGTTTGAGAATCACATAT+TGG 0.388915 2_4:+57769658 None:intergenic
CCATCTTCTTGAGGTTCTGA+TGG 0.391631 2_4:+57769722 None:intergenic
GAAGAATAAACAAGAGAAGA+AGG 0.396694 2_4:-57771800 Msa0337680:CDS
ACCAATGCTTGATGATTGTC+GGG 0.399558 2_4:-57771998 Msa0337680:CDS
AAGAAGATGGAAAATGCTAA+TGG 0.406779 2_4:-57769709 Msa0337680:CDS
CCTTAACAACAACAAATTTG+AGG 0.410672 2_4:-57772295 Msa0337680:five_prime_UTR
ATCAGAATCAGAACCCTTCA+TGG 0.433073 2_4:-57772104 Msa0337680:CDS
ACCACACAAGGGAATGTTAG+AGG 0.439664 2_4:+57772474 None:intergenic
GGAGATAGAAAGATTAAGAC+AGG 0.441141 2_4:-57769752 Msa0337680:CDS
GCATTGTTGTTGTTACTTTG+AGG 0.444336 2_4:+57771679 None:intergenic
TACAAAACTATATAACAAAC+AGG 0.456508 2_4:+57772260 None:intergenic
CCCTCTAACATTCCCTTGTG+TGG 0.457112 2_4:-57772475 Msa0337680:five_prime_UTR
CACCAATGCTTGATGATTGT+CGG 0.462711 2_4:-57771999 Msa0337680:CDS
ATTAACATACAATGTATGAA+TGG 0.472844 2_4:+57772384 None:intergenic
AATGTGGAGTGTGATGGATA+TGG 0.473304 2_4:-57768687 Msa0337680:three_prime_UTR
CATCAGTGGCATCAAAAGAA+AGG 0.482392 2_4:-57768911 Msa0337680:CDS
TTCTGATCTGAAGGACTCGA+AGG 0.485897 2_4:+57771850 None:intergenic
GGTAGAAAGCCAATGCAAAC+AGG 0.488493 2_4:-57771713 Msa0337680:CDS
CCATCAGAACCTCAAGAAGA+TGG 0.490491 2_4:-57769722 Msa0337680:CDS
TTAACATACAATGTATGAAT+GGG 0.503238 2_4:+57772385 None:intergenic
ATGTTTAGTGATGAAATATC+TGG 0.505923 2_4:-57771910 Msa0337680:CDS
TGAAAAGCTTGTCTTGTGCA+AGG 0.507196 2_4:+57770860 None:intergenic
TTATTGGTTGAAATGGATGT+TGG 0.515770 2_4:+57772147 None:intergenic
TTGACAAAATTCTGATCTGA+AGG 0.516864 2_4:+57771841 None:intergenic
CCCCGACAATCATCAAGCAT+TGG 0.530465 2_4:+57771997 None:intergenic
TTTAGGAGAGATGAAGAAAG+AGG 0.538946 2_4:-57771770 Msa0337680:CDS
AGGAACTCATCCACCCATGA+AGG 0.539506 2_4:+57772090 None:intergenic
GAAAAGCTTGTCTTGTGCAA+GGG 0.539760 2_4:+57770861 None:intergenic
ATATGGATAAAATGAAAGAA+AGG 0.562865 2_4:-57768670 Msa0337680:three_prime_UTR
TCATCAAGAGGCACTGAAGA+GGG 0.563124 2_4:-57769773 Msa0337680:CDS
CTGATCCAAAGAGAGTCAAA+AGG 0.572655 2_4:-57771633 Msa0337680:intron
ATCAGTGGCATCAAAAGAAA+GGG 0.574757 2_4:-57768910 Msa0337680:CDS
TTCGATGAAGACAACGTTGG+TGG 0.575587 2_4:+57771874 None:intergenic
TCCCAAACATGTCACCAAGT+TGG 0.580152 2_4:-57772203 Msa0337680:CDS
GGGAAACACATGTGCATCAG+TGG 0.585339 2_4:-57768925 Msa0337680:CDS
AGATATTTCATCACTAAACA+TGG 0.586033 2_4:+57771912 None:intergenic
AAAACAAAGACAATGGCTTG+TGG 0.588542 2_4:-57768710 Msa0337680:intron
GACATGTTTGGGATCTTAGG+AGG 0.588767 2_4:+57772213 None:intergenic
CTCATCAAGAGGCACTGAAG+AGG 0.590175 2_4:-57769774 Msa0337680:CDS
TTGTGGAATGTGGAGTGTGA+TGG 0.590221 2_4:-57768693 Msa0337680:three_prime_UTR
CAAGGCGAGGTGCTCATCGA+AGG 0.594155 2_4:-57772053 Msa0337680:CDS
CCACACAAGGGAATGTTAGA+GGG 0.594577 2_4:+57772475 None:intergenic
TTTACATGCAGCTCATCAAG+AGG 0.601727 2_4:-57769785 Msa0337680:intron
GAATCAGAACCCTTCATGGG+TGG 0.607660 2_4:-57772100 Msa0337680:CDS
ATTGAAATATGAACCTGATG+AGG 0.608223 2_4:-57771734 Msa0337680:CDS
TCAGAATCAGAACCCTTCAT+GGG 0.609308 2_4:-57772103 Msa0337680:CDS
TCTGATCTGAAGGACTCGAA+GGG 0.610267 2_4:+57771851 None:intergenic
ATGAGCACCTCGCCTTGCCG+AGG 0.614435 2_4:+57772059 None:intergenic
AGACAATGGCTTGTGGAATG+TGG 0.619824 2_4:-57768703 Msa0337680:three_prime_UTR
GGAACTCATCCACCCATGAA+GGG 0.626852 2_4:+57772091 None:intergenic
AGTGGCATCAAAAGAAAGGG+TGG 0.627047 2_4:-57768907 Msa0337680:CDS
CAAATAGGCAATCAGCACAA+AGG 0.647617 2_4:-57771205 Msa0337680:CDS
GCGAGGTGCTCATCGAAGGT+CGG 0.657525 2_4:-57772049 Msa0337680:CDS
CAGCACAAAGGTCACGTGTG+AGG 0.661809 2_4:-57771193 Msa0337680:CDS
GCCTTGCCGAGGAAAAGTCG+AGG 0.688307 2_4:+57772070 None:intergenic
GATTGTCGGGGTAAACACGA+TGG 0.708511 2_4:-57771985 Msa0337680:CDS
GGGTTCGATGAAGACAACGT+TGG 0.721008 2_4:+57771871 None:intergenic
ATATTTCCAGAAACCACACA+AGG 0.726763 2_4:+57772462 None:intergenic
CCAATGCTTGATGATTGTCG+GGG 0.745183 2_4:-57771997 Msa0337680:CDS
TATTTCCAGAAACCACACAA+GGG 0.750776 2_4:+57772463 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTATAAAAAAAAAATGTTAA+AGG + chr2_4:57768526-57768545 None:intergenic 10.0%
!!! CTTTAACATTTTTTTTTTAT+AGG - chr2_4:57768524-57768543 Msa0337680:three_prime_UTR 10.0%
!!! TTTTGTTTTTTTAATTAACT+TGG - chr2_4:57768651-57768670 Msa0337680:three_prime_UTR 10.0%
!! GAAATATACATATTTATTAA+AGG - chr2_4:57768710-57768729 Msa0337680:intron 10.0%
!!! GTTTTATTAATAATAATTAC+TGG - chr2_4:57771237-57771256 Msa0337680:intron 10.0%
!! AGATAAAAATTAAAACATGT+AGG - chr2_4:57769573-57769592 Msa0337680:intron 15.0%
!! ATGAAATGACAAAATAAAAA+TGG - chr2_4:57770451-57770470 Msa0337680:intron 15.0%
!! AGAATTAATATCAATATTGA+AGG - chr2_4:57770902-57770921 Msa0337680:CDS 15.0%
!! ATATTTGAAAAATACTTAGT+TGG + chr2_4:57771103-57771122 None:intergenic 15.0%
!! TTTAAATCTGATAAGTAAAA+AGG + chr2_4:57771159-57771178 None:intergenic 15.0%
!! AATTAATGAAAAACAATGAT+TGG + chr2_4:57771263-57771282 None:intergenic 15.0%
!! CTAAATAGTATTAACTATTA+AGG + chr2_4:57771295-57771314 None:intergenic 15.0%
!!! ATTGCAGAATTTTTTTTATA+AGG - chr2_4:57771646-57771665 Msa0337680:CDS 15.0%
!! TCTATATAGAAAAAATTGAA+TGG - chr2_4:57772321-57772340 Msa0337680:five_prime_UTR 15.0%
!! TTAACATACAATGTATGAAT+GGG + chr2_4:57768774-57768793 None:intergenic 20.0%
!! ATTAACATACAATGTATGAA+TGG + chr2_4:57768775-57768794 None:intergenic 20.0%
!! TACAAAACTATATAACAAAC+AGG + chr2_4:57768899-57768918 None:intergenic 20.0%
!!! CATAATATTTTTTTGGTGTT+TGG + chr2_4:57768989-57769008 None:intergenic 20.0%
!!! ATGTTGGCATAATATTTTTT+TGG + chr2_4:57768996-57769015 None:intergenic 20.0%
!! AATTGAAATATTGTTGATCT+TGG - chr2_4:57769820-57769839 Msa0337680:intron 20.0%
!! AAATTTAATAAGAGTGAAAC+AGG - chr2_4:57770548-57770567 Msa0337680:intron 20.0%
!! TATATTAACTACTTTGTGAT+GGG + chr2_4:57770664-57770683 None:intergenic 20.0%
!! AAGTATTTGTACATTCTATT+TGG + chr2_4:57771076-57771095 None:intergenic 20.0%
!!! GTAAAATATCCATTTTGAAA+AGG - chr2_4:57772050-57772069 Msa0337680:CDS 20.0%
!!! ATTTAGTTTCCTTTTCAAAA+TGG + chr2_4:57772062-57772081 None:intergenic 20.0%
!! ATATAGAAAAAATTGAATGG+AGG - chr2_4:57772324-57772343 Msa0337680:five_prime_UTR 20.0%
!!! TTTATCTTTTTCAATCCATT+TGG - chr2_4:57772422-57772441 Msa0337680:five_prime_UTR 20.0%
!! ATATGGATAAAATGAAAGAA+AGG - chr2_4:57772486-57772505 Msa0337680:five_prime_UTR 20.0%
! AGATATTTCATCACTAAACA+TGG + chr2_4:57769247-57769266 None:intergenic 25.0%
! ATGTTTAGTGATGAAATATC+TGG - chr2_4:57769246-57769265 Msa0337680:intron 25.0%
! ACTAAAAAATTGTGTCATGT+GGG + chr2_4:57769714-57769733 None:intergenic 25.0%
! TACTAAAAAATTGTGTCATG+TGG + chr2_4:57769715-57769734 None:intergenic 25.0%
!!! GTTTTTTTGCATTAGAATCT+TGG - chr2_4:57769928-57769947 Msa0337680:intron 25.0%
! ATTTGCAGTACTATAATATC+TGG - chr2_4:57770082-57770101 Msa0337680:intron 25.0%
! ATATATATTTGGACAGTAGA+AGG + chr2_4:57770173-57770192 None:intergenic 25.0%
! ACTGTCCAAATATATATTGA+AGG - chr2_4:57770176-57770195 Msa0337680:intron 25.0%
! TTCAGCCTTCAATATATATT+TGG + chr2_4:57770184-57770203 None:intergenic 25.0%
! ATATTAACTACTTTGTGATG+GGG + chr2_4:57770663-57770682 None:intergenic 25.0%
! GTATATTAACTACTTTGTGA+TGG + chr2_4:57770665-57770684 None:intergenic 25.0%
!!! CTCTTGAAATTTTGATACAT+AGG + chr2_4:57770765-57770784 None:intergenic 25.0%
! GTTAGTCAAAATAGTTTCTT+AGG - chr2_4:57770803-57770822 Msa0337680:intron 25.0%
!! AAGGAAAATGAGAGATTTTA+AGG - chr2_4:57771665-57771684 Msa0337680:CDS 25.0%
!!! AAATGAGAGATTTTAAGGAA+AGG - chr2_4:57771670-57771689 Msa0337680:CDS 25.0%
!!! AATGAGAGATTTTAAGGAAA+GGG - chr2_4:57771671-57771690 Msa0337680:CDS 25.0%
! AAAATAGCGAACTTAAAACT+TGG - chr2_4:57771800-57771819 Msa0337680:CDS 25.0%
!!! TGTGTTTTGCTTTAAAAGAA+AGG - chr2_4:57772177-57772196 Msa0337680:CDS 25.0%
!!! GTGTTTTGCTTTAAAAGAAA+GGG - chr2_4:57772178-57772197 Msa0337680:CDS 25.0%
!!! ATTGTCTTTGTTTTTCCAAA+TGG + chr2_4:57772440-57772459 None:intergenic 25.0%
! ATTTGGAAAAACAAAGACAA+TGG - chr2_4:57772439-57772458 Msa0337680:five_prime_UTR 25.0%
! AAATTGCGCTGTATATATAA+TGG - chr2_4:57772626-57772645 Msa0337680:five_prime_UTR 25.0%
TTATTAAAGGGTTGTGTTAG+AGG - chr2_4:57768723-57768742 Msa0337680:intron 30.0%
!! CCTCAAATTTGTTGTTGTTA+AGG + chr2_4:57768864-57768883 None:intergenic 30.0%
CCTTAACAACAACAAATTTG+AGG - chr2_4:57768861-57768880 Msa0337680:intron 30.0%
! TTTTGTACATCTCTCTCAAA+TGG - chr2_4:57768912-57768931 Msa0337680:CDS 30.0%
!!! TTTTGGTGTTTGGAAAAATC+TGG + chr2_4:57768979-57768998 None:intergenic 30.0%
! TTATTGGTTGAAATGGATGT+TGG + chr2_4:57769012-57769031 None:intergenic 30.0%
! GACAATGTTATTGGTTGAAA+TGG + chr2_4:57769019-57769038 None:intergenic 30.0%
GTTTGTAGTGACAATGTTAT+TGG + chr2_4:57769028-57769047 None:intergenic 30.0%
TTGACAAAATTCTGATCTGA+AGG + chr2_4:57769318-57769337 None:intergenic 30.0%
GAAGAATAAACAAGAGAAGA+AGG - chr2_4:57769356-57769375 Msa0337680:intron 30.0%
GAGAAGAAGGAAAATGATTT+AGG - chr2_4:57769369-57769388 Msa0337680:intron 30.0%
ATTGAAATATGAACCTGATG+AGG - chr2_4:57769422-57769441 Msa0337680:intron 30.0%
!! AATTACCTTTTGACTCTCTT+TGG + chr2_4:57769531-57769550 None:intergenic 30.0%
CAGTGCTATATATAGCTTAT+TGG + chr2_4:57770487-57770506 None:intergenic 30.0%
TATTAACTACTTTGTGATGG+GGG + chr2_4:57770662-57770681 None:intergenic 30.0%
ATTATTGTGCTTACTCGTTA+GGG - chr2_4:57770925-57770944 Msa0337680:CDS 30.0%
TACTATTTAGACACATAGCT+AGG - chr2_4:57771305-57771324 Msa0337680:intron 30.0%
ACTATTTAGACACATAGCTA+GGG - chr2_4:57771306-57771325 Msa0337680:intron 30.0%
AAGAAGATGGAAAATGCTAA+TGG - chr2_4:57771447-57771466 Msa0337680:intron 30.0%
TCTGTTTGAGAATCACATAT+TGG + chr2_4:57771501-57771520 None:intergenic 30.0%
AAGGAAAGGGAAAATAAATG+AGG - chr2_4:57771684-57771703 Msa0337680:CDS 30.0%
AGGAAAGGGAAAATAAATGA+GGG - chr2_4:57771685-57771704 Msa0337680:CDS 30.0%
ATTGTTTAATCTTACTCGCA+CGG - chr2_4:57771823-57771842 Msa0337680:CDS 30.0%
TAGATATGACTGACAACATA+TGG - chr2_4:57771848-57771867 Msa0337680:CDS 30.0%
ATAATCGACTGTAAATTCAG+CGG + chr2_4:57771977-57771996 None:intergenic 30.0%
!!! ACTTGTTTTTATGCCACATT+TGG + chr2_4:57772030-57772049 None:intergenic 30.0%
TAGTACTACTAGTACAAGTA+GGG - chr2_4:57772121-57772140 Msa0337680:CDS 30.0%
! TTTGAGGTCTTGGATTTAAT+TGG - chr2_4:57772518-57772537 Msa0337680:five_prime_UTR 30.0%
TATTTCCAGAAACCACACAA+GGG + chr2_4:57768696-57768715 None:intergenic 35.0%
ATATTTCCAGAAACCACACA+AGG + chr2_4:57768697-57768716 None:intergenic 35.0%
TTTAGGAGAGATGAAGAAAG+AGG - chr2_4:57769386-57769405 Msa0337680:intron 35.0%
! GTGAATTTTCCTGTTTGCAT+TGG + chr2_4:57769455-57769474 None:intergenic 35.0%
!! GCATTGTTGTTGTTACTTTG+AGG + chr2_4:57769480-57769499 None:intergenic 35.0%
GAATCAGTTGCAAAACTCAA+AGG + chr2_4:57769906-57769925 None:intergenic 35.0%
!! TTGAGTTTTGCAACTGATTC+TGG - chr2_4:57769906-57769925 Msa0337680:intron 35.0%
GCATTAGAATCTTGGCAAAT+AGG - chr2_4:57769936-57769955 Msa0337680:intron 35.0%
! ATCTCCAAGAGTTGCATTTT+TGG - chr2_4:57770215-57770234 Msa0337680:intron 35.0%
TGATCCAAAAATGCAACTCT+TGG + chr2_4:57770222-57770241 None:intergenic 35.0%
! CAAGACAAGCTTTTCAAAGA+TGG - chr2_4:57770303-57770322 Msa0337680:intron 35.0%
!! ATTTCATGCTTGACTTGTCA+TGG + chr2_4:57770438-57770457 None:intergenic 35.0%
AGTCTACTAACATTTGAGCT+CGG - chr2_4:57770691-57770710 Msa0337680:intron 35.0%
GATTATTGTGCTTACTCGTT+AGG - chr2_4:57770924-57770943 Msa0337680:CDS 35.0%
TATGTTTGTGTGCGTTCAAA+TGG + chr2_4:57770968-57770987 None:intergenic 35.0%
AAAAGGAGCTAACTACTTTG+AGG + chr2_4:57771142-57771161 None:intergenic 35.0%
GGAGATAGAAAGATTAAGAC+AGG - chr2_4:57771404-57771423 Msa0337680:intron 35.0%
! TAGCATTTTCCATCTTCTTG+AGG + chr2_4:57771446-57771465 None:intergenic 35.0%
!!! ACATATTGGCTTTTGTGATG+GGG + chr2_4:57771487-57771506 None:intergenic 35.0%
!!! CACATATTGGCTTTTGTGAT+GGG + chr2_4:57771488-57771507 None:intergenic 35.0%
!!! TCACATATTGGCTTTTGTGA+TGG + chr2_4:57771489-57771508 None:intergenic 35.0%
CACACGGTTACAATTACATA+AGG + chr2_4:57771736-57771755 None:intergenic 35.0%
AATAGAACATGAGTTGCATG+CGG + chr2_4:57771888-57771907 None:intergenic 35.0%
AGACCGTTCGATTAATCTAA+CGG - chr2_4:57771997-57772016 Msa0337680:CDS 35.0%
AGACCGTTAGATTAATCGAA+CGG + chr2_4:57772003-57772022 None:intergenic 35.0%
TTTGAAGAGTCACACGTTAT+TGG - chr2_4:57772086-57772105 Msa0337680:CDS 35.0%
TTGAAGAGTCACACGTTATT+GGG - chr2_4:57772087-57772106 Msa0337680:CDS 35.0%
GTAGTACTACTAGTACAAGT+AGG - chr2_4:57772120-57772139 Msa0337680:CDS 35.0%
ATCAGTGGCATCAAAAGAAA+GGG - chr2_4:57772246-57772265 Msa0337680:exon 35.0%
AAAACAAAGACAATGGCTTG+TGG - chr2_4:57772446-57772465 Msa0337680:five_prime_UTR 35.0%
GGTCTTGGATTTAATTGGTT+TGG - chr2_4:57772523-57772542 Msa0337680:five_prime_UTR 35.0%
TTGGAAAAATCTGGCCAACT+TGG + chr2_4:57768970-57768989 None:intergenic 40.0%
ATCAGAATCAGAACCCTTCA+TGG - chr2_4:57769052-57769071 Msa0337680:intron 40.0%
TCAGAATCAGAACCCTTCAT+GGG - chr2_4:57769053-57769072 Msa0337680:intron 40.0%
! CACCAATGCTTGATGATTGT+CGG - chr2_4:57769157-57769176 Msa0337680:intron 40.0%
! ACCAATGCTTGATGATTGTC+GGG - chr2_4:57769158-57769177 Msa0337680:intron 40.0%
CTGATCCAAAGAGAGTCAAA+AGG - chr2_4:57769523-57769542 Msa0337680:intron 40.0%
CAAATAGGCAATCAGCACAA+AGG - chr2_4:57769951-57769970 Msa0337680:intron 40.0%
GAAAAGCTTGTCTTGTGCAA+GGG + chr2_4:57770298-57770317 None:intergenic 40.0%
TGAAAAGCTTGTCTTGTGCA+AGG + chr2_4:57770299-57770318 None:intergenic 40.0%
GTGAAAGAATATGTACCCTG+TGG - chr2_4:57770401-57770420 Msa0337680:intron 40.0%
! TGACTTGTCATGGTCTCATT+AGG + chr2_4:57770428-57770447 None:intergenic 40.0%
GCACTGTAGTTACTTGAAGT+TGG - chr2_4:57770501-57770520 Msa0337680:intron 40.0%
GTTGGTCAAGTATTCACTAG+TGG - chr2_4:57770519-57770538 Msa0337680:intron 40.0%
TCTGTGCAGTCTCATAACTA+TGG + chr2_4:57770575-57770594 None:intergenic 40.0%
GGGCACATGATCACATAAAT+AGG + chr2_4:57770642-57770661 None:intergenic 40.0%
GTCTACTAACATTTGAGCTC+GGG - chr2_4:57770692-57770711 Msa0337680:intron 40.0%
TTTACATGCAGCTCATCAAG+AGG - chr2_4:57771371-57771390 Msa0337680:intron 40.0%
!! TTTTGTGATGGGGATGGTAA+GGG + chr2_4:57771477-57771496 None:intergenic 40.0%
GACTGACAACATATGGTTAG+AGG - chr2_4:57771855-57771874 Msa0337680:CDS 40.0%
TAACGGTCTACAACCAAATG+TGG - chr2_4:57772014-57772033 Msa0337680:CDS 40.0%
CCAATCATGCAAACTTTGAG+TGG + chr2_4:57772205-57772224 None:intergenic 40.0%
CCACTCAAAGTTTGCATGAT+TGG - chr2_4:57772202-57772221 Msa0337680:CDS 40.0%
AGTTTGCATGATTGGAATGC+AGG - chr2_4:57772210-57772229 Msa0337680:CDS 40.0%
GTTTGCATGATTGGAATGCA+GGG - chr2_4:57772211-57772230 Msa0337680:CDS 40.0%
CATCAGTGGCATCAAAAGAA+AGG - chr2_4:57772245-57772264 Msa0337680:exon 40.0%
!! AATGTGGAGTGTGATGGATA+TGG - chr2_4:57772469-57772488 Msa0337680:five_prime_UTR 40.0%
!! AGAAAGGTGTGTCAGTTTTG+AGG - chr2_4:57772502-57772521 Msa0337680:five_prime_UTR 40.0%
CCACACAAGGGAATGTTAGA+GGG + chr2_4:57768684-57768703 None:intergenic 45.0%
ACCACACAAGGGAATGTTAG+AGG + chr2_4:57768685-57768704 None:intergenic 45.0%
ACATTCCCTTGTGTGGTTTC+TGG - chr2_4:57768688-57768707 Msa0337680:three_prime_UTR 45.0%
! GACATGTTTGGGATCTTAGG+AGG + chr2_4:57768946-57768965 None:intergenic 45.0%
! GGTGACATGTTTGGGATCTT+AGG + chr2_4:57768949-57768968 None:intergenic 45.0%
TCCCAAACATGTCACCAAGT+TGG - chr2_4:57768953-57768972 Msa0337680:intron 45.0%
! GCCAACTTGGTGACATGTTT+GGG + chr2_4:57768957-57768976 None:intergenic 45.0%
! TGAGTTCCTCGACTTTTCCT+CGG - chr2_4:57769080-57769099 Msa0337680:intron 45.0%
! CCAATGCTTGATGATTGTCG+GGG - chr2_4:57769159-57769178 Msa0337680:intron 45.0%
TTCGATGAAGACAACGTTGG+TGG + chr2_4:57769285-57769304 None:intergenic 45.0%
TCTGATCTGAAGGACTCGAA+GGG + chr2_4:57769308-57769327 None:intergenic 45.0%
TTCTGATCTGAAGGACTCGA+AGG + chr2_4:57769309-57769328 None:intergenic 45.0%
!! CATTGGCTTTCTACCTCATC+AGG + chr2_4:57769438-57769457 None:intergenic 45.0%
GGTAGAAAGCCAATGCAAAC+AGG - chr2_4:57769443-57769462 Msa0337680:intron 45.0%
GCGTAGTGTAACTTCATTGC+AGG - chr2_4:57770012-57770031 Msa0337680:intron 45.0%
ATGGTCTCATTAGGACCACA+GGG + chr2_4:57770419-57770438 None:intergenic 45.0%
!! TCATCAAGAGGCACTGAAGA+GGG - chr2_4:57771383-57771402 Msa0337680:intron 45.0%
! CCATCTTCTTGAGGTTCTGA+TGG + chr2_4:57771437-57771456 None:intergenic 45.0%
CCATCAGAACCTCAAGAAGA+TGG - chr2_4:57771434-57771453 Msa0337680:intron 45.0%
!! CTTTTGTGATGGGGATGGTA+AGG + chr2_4:57771478-57771497 None:intergenic 45.0%
!!! ATTGGCTTTTGTGATGGGGA+TGG + chr2_4:57771483-57771502 None:intergenic 45.0%
TAACCGTGTGCATAGTCAGT+CGG - chr2_4:57771746-57771765 Msa0337680:CDS 45.0%
AAGAGTCACACGTTATTGGG+TGG - chr2_4:57772090-57772109 Msa0337680:CDS 45.0%
AGTGGCATCAAAAGAAAGGG+TGG - chr2_4:57772249-57772268 Msa0337680:five_prime_UTR 45.0%
! AGACAATGGCTTGTGGAATG+TGG - chr2_4:57772453-57772472 Msa0337680:five_prime_UTR 45.0%
TTGTGGAATGTGGAGTGTGA+TGG - chr2_4:57772463-57772482 Msa0337680:five_prime_UTR 45.0%
!!! GTGTGTCAGTTTTGAGGTCT+TGG - chr2_4:57772508-57772527 Msa0337680:five_prime_UTR 45.0%
!! TATAAAAAAAAAATGTTAAA+GGG + chr2_4:57768525-57768544 None:intergenic 5.0%
!!! ATTTTACTAAATTTTATTTA+GGG + chr2_4:57768561-57768580 None:intergenic 5.0%
!!! AATTTTACTAAATTTTATTT+AGG + chr2_4:57768562-57768581 None:intergenic 5.0%
!! AAATATACATATTTATTAAA+GGG - chr2_4:57768711-57768730 Msa0337680:intron 5.0%
CCCTCTAACATTCCCTTGTG+TGG - chr2_4:57768681-57768700 Msa0337680:three_prime_UTR 50.0%
! GGCCAACTTGGTGACATGTT+TGG + chr2_4:57768958-57768977 None:intergenic 50.0%
GAATCAGAACCCTTCATGGG+TGG - chr2_4:57769056-57769075 Msa0337680:intron 50.0%
GGAACTCATCCACCCATGAA+GGG + chr2_4:57769068-57769087 None:intergenic 50.0%
AGGAACTCATCCACCCATGA+AGG + chr2_4:57769069-57769088 None:intergenic 50.0%
CCCCGACAATCATCAAGCAT+TGG + chr2_4:57769162-57769181 None:intergenic 50.0%
GATTGTCGGGGTAAACACGA+TGG - chr2_4:57769171-57769190 Msa0337680:intron 50.0%
GGGTTCGATGAAGACAACGT+TGG + chr2_4:57769288-57769307 None:intergenic 50.0%
CATGGTCTCATTAGGACCAC+AGG + chr2_4:57770420-57770439 None:intergenic 50.0%
!! GGAGCTAACTACTTTGAGGC+AGG + chr2_4:57771138-57771157 None:intergenic 50.0%
!! CTCATCAAGAGGCACTGAAG+AGG - chr2_4:57771382-57771401 Msa0337680:intron 50.0%
TGACCGACTGACTATGCACA+CGG + chr2_4:57771752-57771771 None:intergenic 50.0%
CATAGTCAGTCGGTCACATC+TGG - chr2_4:57771756-57771775 Msa0337680:CDS 50.0%
TCACACGTTATTGGGTGGTG+AGG - chr2_4:57772095-57772114 Msa0337680:CDS 50.0%
GGGAAACACATGTGCATCAG+TGG - chr2_4:57772231-57772250 Msa0337680:exon 50.0%
! TCCTCGACTTTTCCTCGGCA+AGG - chr2_4:57769085-57769104 Msa0337680:intron 55.0%
CAGCACAAAGGTCACGTGTG+AGG - chr2_4:57769963-57769982 Msa0337680:intron 55.0%
GCCTTGCCGAGGAAAAGTCG+AGG + chr2_4:57769089-57769108 None:intergenic 60.0%
! GACTTTTCCTCGGCAAGGCG+AGG - chr2_4:57769090-57769109 Msa0337680:intron 60.0%
! CAAGGCGAGGTGCTCATCGA+AGG - chr2_4:57769103-57769122 Msa0337680:intron 60.0%
! GCGAGGTGCTCATCGAAGGT+CGG - chr2_4:57769107-57769126 Msa0337680:intron 60.0%
ATGAGCACCTCGCCTTGCCG+AGG + chr2_4:57769100-57769119 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 57768522 57772656 57768522 ID=Msa0337680;Name=Msa0337680
chr2_4 mRNA 57768522 57772656 57768522 ID=Msa0337680-mRNA-1;Parent=Msa0337680;Name=Msa0337680-mRNA-1;_AED=0.07;_eAED=0.07;_QI=409|1|1|1|0.8|0.66|6|203|352
chr2_4 exon 57768522 57768723 57768522 ID=Msa0337680-mRNA-1:exon:17621;Parent=Msa0337680-mRNA-1
chr2_4 exon 57768877 57768946 57768877 ID=Msa0337680-mRNA-1:exon:17620;Parent=Msa0337680-mRNA-1
chr2_4 exon 57769639 57769796 57769639 ID=Msa0337680-mRNA-1:exon:17619;Parent=Msa0337680-mRNA-1
chr2_4 exon 57770854 57770980 57770854 ID=Msa0337680-mRNA-1:exon:17618;Parent=Msa0337680-mRNA-1
chr2_4 exon 57771145 57771235 57771145 ID=Msa0337680-mRNA-1:exon:17617;Parent=Msa0337680-mRNA-1
chr2_4 exon 57771634 57772656 57771634 ID=Msa0337680-mRNA-1:exon:17616;Parent=Msa0337680-mRNA-1
chr2_4 five_prime_UTR 57772248 57772656 57772248 ID=Msa0337680-mRNA-1:five_prime_utr;Parent=Msa0337680-mRNA-1
chr2_4 CDS 57771634 57772247 57771634 ID=Msa0337680-mRNA-1:cds;Parent=Msa0337680-mRNA-1
chr2_4 CDS 57771145 57771235 57771145 ID=Msa0337680-mRNA-1:cds;Parent=Msa0337680-mRNA-1
chr2_4 CDS 57770854 57770980 57770854 ID=Msa0337680-mRNA-1:cds;Parent=Msa0337680-mRNA-1
chr2_4 CDS 57769639 57769796 57769639 ID=Msa0337680-mRNA-1:cds;Parent=Msa0337680-mRNA-1
chr2_4 CDS 57768878 57768946 57768878 ID=Msa0337680-mRNA-1:cds;Parent=Msa0337680-mRNA-1
chr2_4 three_prime_UTR 57768877 57768877 57768877 ID=Msa0337680-mRNA-1:three_prime_utr;Parent=Msa0337680-mRNA-1
chr2_4 three_prime_UTR 57768522 57768723 57768522 ID=Msa0337680-mRNA-1:three_prime_utr;Parent=Msa0337680-mRNA-1
Gene Sequence

>Msa0337680

ATGGCACAATTACCTCCTAAGATCCCAAACATGTCACCAAGTTGGCCAGATTTTTCCAAACACCAAAAAAATATTATGCCAACATCCATTTCAACCAATAACATTGTCACTACAAACAATTATCAGAATCAGAACCCTTCATGGGTGGATGAGTTCCTCGACTTTTCCTCGGCAAGGCGAGGTGCTCATCGAAGGTCGGTCAGCGATTCAGTCACATTCTTAGAAACACCAATGCTTGATGATTGTCGGGGTAAACACGATGGTAACAACAATGACAACGAGTTCGACAAGTTTGATGATGAACAATTTTTGTCCATGTTTAGTGATGAAATATCTGGTGTCAATAACATGCCACCAACGTTGTCTTCATCGAACCCTTCGAGTCCTTCAGATCAGAATTTTGTCAATGATGATAATGAGAAAGAGAAGAATAAACAAGAGAAGAAGGAAAATGATTTAGGAGAGATGAAGAAAGAGGAAGATGAGAAGCAATTGAAATATGAACCTGATGAGGTAGAAAGCCAATGCAAACAGGAAAATTCACAGCCTCAAAGTAACAACAACAATGCAACTTCTTCAAATGATAGAATTACTGATCCAAAGAGAGTCAAAAGAATCTTGGCAAATAGGCAATCAGCACAAAGGTCACGTGTGAGGAAGCTGCAATACATATCAGAGCTTGAGCGTAGTGTAACTTCATTGCAGGCTGAAGTTTCAGTGCTATCTCCAAGAGTTGCATTTTTGGATCATCAACGATTGCTTCTAAATGTTGACAACAGTGCTCTCAAGCAAAGAATTGCAGCCCTTGCACAAGACAAGCTTTTCAAAGATGCTCATCAAGAGGCACTGAAGAGGGAGATAGAAAGATTAAGACAGGTATATCACCATCAGAACCTCAAGAAGATGGAAAATGCTAATGGATCACCCTTACCATCCCCATCACAAAAGCCAATATGTGATTCTCAAACAGAAAGCGAACAACTACTCAATGGAAACACATGTGCATCAGTGGCATCAAAAGAAAGGGTGGAAGAAACAGTTGTGTCTGCTTTAACATGA
Protein sequence

>Msa0337680

MAQLPPKIPNMSPSWPDFSKHQKNIMPTSISTNNIVTTNNYQNQNPSWVDEFLDFSSARRGAHRRSVSDSVTFLETPMLDDCRGKHDGNNNDNEFDKFDDEQFLSMFSDEISGVNNMPPTLSSSNPSSPSDQNFVNDDNEKEKNKQEKKENDLGEMKKEEDEKQLKYEPDEVESQCKQENSQPQSNNNNATSSNDRITDPKRVKRILANRQSAQRSRVRKLQYISELERSVTSLQAEVSVLSPRVAFLDHQRLLLNVDNSALKQRIAALAQDKLFKDAHQEALKREIERLRQVYHHQNLKKMENANGSPLPSPSQKPICDSQTESEQLLNGNTCASVASKERVEETVVSALT*