AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0352070

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0352070 MtrunA17_Chr3g0087291 92.832 572 17 2 1 562 1 558 0.0 1027
Msa0352070 MtrunA17_Chr4g0010511 68.590 624 134 8 1 565 1 621 0.0 788
Msa0352070 MtrunA17_Chr4g0070551 36.235 425 255 7 145 560 200 617 7.76e-85 276
Msa0352070 MtrunA17_Chr5g0426591 37.528 453 236 12 142 563 78 514 7.86e-84 271
Msa0352070 MtrunA17_Chr3g0129981 36.591 440 246 11 142 560 84 511 3.86e-81 263
Msa0352070 MtrunA17_Chr4g0008451 34.713 435 262 9 139 561 198 622 2.61e-78 259
Msa0352070 MtrunA17_Chr5g0448581 36.321 424 251 9 146 562 90 501 8.88e-78 256
Msa0352070 MtrunA17_Chr3g0132371 35.255 451 237 10 146 564 115 542 9.13e-76 250
Msa0352070 MtrunA17_Chr4g0056061 37.984 387 226 8 180 560 111 489 1.17e-75 249
Msa0352070 MtrunA17_Chr6g0455571 37.968 374 218 6 180 543 118 487 2.54e-75 248
Msa0352070 MtrunA17_Chr5g0399311 35.165 455 279 9 112 560 61 505 5.38e-75 247
Msa0352070 MtrunA17_Chr3g0143821 35.345 464 265 15 117 562 48 494 6.93e-75 248
Msa0352070 MtrunA17_Chr5g0436321 36.410 390 236 6 180 560 164 550 1.42e-73 244
Msa0352070 MtrunA17_Chr4g0042711 36.203 395 233 9 180 560 100 489 6.04e-72 239
Msa0352070 MtrunA17_Chr5g0412251 34.244 476 252 11 130 560 42 501 1.47e-71 238
Msa0352070 MtrunA17_Chr4g0061781 36.898 374 220 7 180 542 119 487 2.11e-70 235
Msa0352070 MtrunA17_Chr4g0043911 33.557 447 263 11 145 568 124 559 5.68e-70 235
Msa0352070 MtrunA17_Chr2g0307971 36.413 368 220 6 180 537 118 481 2.93e-69 232
Msa0352070 MtrunA17_Chr5g0417871 35.264 397 238 8 180 562 88 479 5.64e-68 228
Msa0352070 MtrunA17_Chr1g0160781 33.562 438 261 10 145 563 123 549 9.94e-67 226
Msa0352070 MtrunA17_Chr3g0132951 32.759 464 243 9 145 560 73 515 3.93e-66 224
Msa0352070 MtrunA17_Chr5g0424241 33.578 408 244 6 180 564 160 563 7.16e-66 225
Msa0352070 MtrunA17_Chr1g0204121 30.093 432 281 9 146 567 61 481 1.72e-65 222
Msa0352070 MtrunA17_Chr3g0123521 32.883 444 267 12 140 560 112 547 2.93e-65 222
Msa0352070 MtrunA17_Chr3g0077321 32.558 430 265 9 148 560 123 544 5.52e-63 216
Msa0352070 MtrunA17_Chr1g0204141 28.440 436 286 11 146 567 61 484 5.08e-55 194
Msa0352070 MtrunA17_Chr2g0284071 29.185 466 273 15 144 560 183 640 8.23e-47 174
Msa0352070 MtrunA17_Chr4g0075311 30.162 431 245 12 180 560 167 591 9.61e-46 170
Msa0352070 MtrunA17_Chr4g0003561 27.083 384 254 11 180 560 78 438 2.24e-33 132
Msa0352070 MtrunA17_Chr7g0220381 30.000 290 188 6 180 454 101 390 4.77e-31 128
Msa0352070 MtrunA17_Chr1g0146501 29.932 294 191 6 180 458 93 386 4.71e-29 122
Msa0352070 MtrunA17_Chr3g0127811 28.037 321 208 8 152 458 35 346 5.41e-27 114
Msa0352070 MtrunA17_Chr4g0002361 25.581 344 195 13 180 505 89 389 1.51e-20 94.4
Msa0352070 MtrunA17_Chr2g0298881 24.619 394 227 16 145 513 5 353 1.69e-19 90.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0352070 AT2G44500.1 68.271 561 155 6 11 562 17 563 0.0 758
Msa0352070 AT3G07900.1 61.728 567 205 7 10 566 14 578 0.0 682
Msa0352070 AT5G63390.1 61.779 416 158 1 146 560 133 548 0.0 559
Msa0352070 AT2G44500.2 64.604 404 120 6 11 405 17 406 9.03e-171 491
Msa0352070 AT5G35570.1 35.831 427 258 7 145 562 223 642 8.17e-80 264
Msa0352070 AT1G22460.1 35.253 434 258 5 146 563 134 560 1.55e-78 258
Msa0352070 AT5G64600.1 36.054 441 245 10 144 561 89 515 2.21e-78 256
Msa0352070 AT1G20550.1 35.211 426 263 9 141 562 75 491 2.63e-76 252
Msa0352070 AT5G01100.1 34.346 428 261 8 146 561 190 609 3.93e-76 253
Msa0352070 AT3G54100.1 34.107 431 256 9 146 561 195 612 2.83e-75 251
Msa0352070 AT1G76270.1 34.803 431 258 8 141 562 72 488 3.93e-74 247
Msa0352070 AT4G24530.1 34.395 471 240 11 138 560 67 516 1.43e-72 241
Msa0352070 AT2G37980.1 33.568 426 265 8 146 561 199 616 2.57e-72 243
Msa0352070 AT2G03280.1 37.297 370 222 5 181 542 92 459 1.94e-71 237
Msa0352070 AT5G15740.1 36.461 373 223 6 180 542 109 477 4.25e-71 237
Msa0352070 AT5G65470.1 33.544 474 256 10 132 561 45 503 8.55e-71 236
Msa0352070 AT4G38390.1 36.997 373 214 6 179 540 125 487 7.25e-70 235
Msa0352070 AT1G14020.1 35.550 391 239 6 181 560 95 483 1.06e-68 230
Msa0352070 AT1G14970.2 32.444 450 276 8 137 568 1 440 1.71e-68 228
Msa0352070 AT3G02250.1 35.849 371 228 5 180 542 109 477 1.84e-68 230
Msa0352070 AT1G14970.1 32.511 446 273 8 141 568 125 560 1.06e-66 227
Msa0352070 AT4G16650.2 39.175 388 220 10 180 560 125 503 6.44e-66 224
Msa0352070 AT4G16650.1 39.175 388 220 10 180 560 125 503 6.44e-66 224
Msa0352070 AT2G03280.2 34.761 397 222 6 181 542 92 486 8.86e-66 223
Msa0352070 AT1G14970.3 32.933 416 259 6 170 568 2 414 1.70e-65 219
Msa0352070 AT3G26370.1 34.422 398 241 9 180 560 161 555 1.04e-64 221
Msa0352070 AT1G38065.1 33.738 412 230 10 180 560 39 438 6.56e-63 214
Msa0352070 AT1G38065.2 33.738 412 230 10 180 560 29 428 6.91e-63 214
Msa0352070 AT1G35510.1 31.435 439 275 8 146 567 134 563 2.15e-62 215
Msa0352070 AT2G01480.1 32.723 437 266 8 141 559 124 550 3.29e-62 215
Msa0352070 AT1G38131.1 33.738 412 230 10 180 560 158 557 1.20e-61 214
Msa0352070 AT1G04910.1 29.907 428 273 11 146 560 61 474 1.18e-60 209
Msa0352070 AT2G01480.2 33.165 395 241 6 141 519 124 511 2.05e-57 201
Msa0352070 AT1G62330.1 29.958 474 261 15 146 563 194 652 1.47e-55 198
Msa0352070 AT1G11990.1 30.664 437 276 11 144 561 161 589 1.04e-52 189
Msa0352070 AT1G11990.2 28.270 474 276 11 144 561 146 611 2.55e-47 175
Msa0352070 AT1G29200.4 31.567 453 253 14 156 560 50 493 1.77e-46 170
Msa0352070 AT1G29200.1 31.567 453 253 14 156 560 50 493 1.77e-46 170
Msa0352070 AT1G29200.2 31.567 453 253 14 156 560 166 609 6.36e-46 171
Msa0352070 AT1G29200.3 32.084 427 234 13 182 560 1 419 1.47e-45 166
Msa0352070 AT3G30300.2 32.990 291 178 6 180 454 103 392 9.64e-35 139
Msa0352070 AT3G30300.1 32.990 291 178 6 180 454 103 392 9.64e-35 139
Msa0352070 AT1G52630.1 24.122 427 281 14 143 560 43 435 2.24e-30 124
Msa0352070 AT3G26370.2 35.165 182 104 4 180 348 161 341 8.83e-30 121
Msa0352070 AT3G03810.2 31.525 295 185 7 180 458 92 385 1.19e-29 124
Msa0352070 AT3G03810.1 31.525 295 185 7 180 458 92 385 1.19e-29 124
Msa0352070 AT1G52630.2 24.138 377 246 13 193 560 2 347 1.17e-25 108

Find 145 sgRNAs with CRISPR-Local

Find 249 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAAATTCAAGTGTTATTT+TGG 0.098612 3_1:+19773600 Msa0352070:three_prime_UTR
TATCTTATGGTTGTTGTTTC+TGG 0.112650 3_1:+19770761 Msa0352070:CDS
CTGTTTCTTGTTGGTTTCTT+TGG 0.136955 3_1:+19770389 Msa0352070:CDS
ACATCTAATCACCTTTATTT+TGG 0.173467 3_1:+19773774 Msa0352070:three_prime_UTR
TTAGGAAACTCTTGGATTAA+TGG 0.176133 3_1:-19772973 None:intergenic
AGTGAAGAATTTAGAAAATA+AGG 0.209967 3_1:-19773374 None:intergenic
TGCTTTGATTGTAGTGAATT+TGG 0.215852 3_1:+19771221 Msa0352070:intron
GCCATCTCATGGAGGAAATA+TGG 0.218103 3_1:+19773110 Msa0352070:CDS
GTTGTTATTGCTAGGATTCT+TGG 0.223923 3_1:+19770821 Msa0352070:CDS
TAACTTCTTTGCATTGTTCT+TGG 0.233140 3_1:-19770234 None:intergenic
TCAACACTTATAATTGTTAT+TGG 0.237204 3_1:+19773838 Msa0352070:three_prime_UTR
TCAACCTAACTAAGTATTAA+TGG 0.239891 3_1:-19773958 None:intergenic
CAGAATACTAAGACCAAGTT+TGG 0.250420 3_1:-19770510 None:intergenic
TAGGATTCTTGGTGCTTCTT+TGG 0.263675 3_1:+19770832 Msa0352070:CDS
TTTGCAAGTCAATGTTATTT+GGG 0.282289 3_1:+19770865 Msa0352070:CDS
CTAACACAAGATTCTGGTTT+AGG 0.285401 3_1:+19773556 Msa0352070:three_prime_UTR
TATAAACCTTGTTTGAGTTT+TGG 0.288062 3_1:+19770689 Msa0352070:CDS
CAAAAGGGTCTAGTGAGTTT+TGG 0.293647 3_1:+19770645 Msa0352070:CDS
AAATATGGGCCGTGCAATTC+AGG 0.296659 3_1:+19773125 Msa0352070:CDS
TTCTTTGGTATGTTCAAGTT+TGG 0.303269 3_1:+19770404 Msa0352070:CDS
TAAGAATGGAAAAGGATGTT+TGG 0.316903 3_1:+19772374 Msa0352070:CDS
CAGGGTCATAGGGCATTTAC+TGG 0.325806 3_1:+19773311 Msa0352070:intron
TTGGAAGAAACCAAATGGTT+TGG 0.327696 3_1:+19770664 Msa0352070:CDS
AAGCACTTCAATTTCCTTCC+AGG 0.337343 3_1:+19773652 Msa0352070:three_prime_UTR
TACACACTAACACAAGATTC+TGG 0.337783 3_1:+19773550 Msa0352070:three_prime_UTR
CACATAAGAATCTCTCTATA+TGG 0.338807 3_1:+19770131 None:intergenic
ACTCCCATTAATACTTAGTT+AGG 0.340251 3_1:+19773954 Msa0352070:three_prime_UTR
TGGAAGAAACCAAATGGTTT+GGG 0.343688 3_1:+19770665 Msa0352070:CDS
CTCAGAGACTATGTAATCTA+TGG 0.350975 3_1:-19773070 None:intergenic
TTTCTTGCTCTGTTTCTTGT+TGG 0.361638 3_1:+19770380 Msa0352070:CDS
AAGAATGGAAAAGGATGTTT+GGG 0.365024 3_1:+19772375 Msa0352070:CDS
TGTCGGTGCTTCAGGGTCAT+AGG 0.368740 3_1:+19773300 Msa0352070:intron
TGAGAACTTCTGAAGATTTG+TGG 0.370747 3_1:-19770465 None:intergenic
GGAAGCATGTGTCTCTTGTT+TGG 0.373927 3_1:-19773353 None:intergenic
TCACAAGAAAATCAACACTA+TGG 0.387562 3_1:+19770357 Msa0352070:CDS
CCTTGCTCTTCATTTAAGAA+TGG 0.389315 3_1:+19772360 Msa0352070:CDS
CAACCTAACTAAGTATTAAT+GGG 0.392952 3_1:-19773957 None:intergenic
CTTCTAATTGAAGACATGTT+TGG 0.394301 3_1:+19773716 Msa0352070:three_prime_UTR
GAGAATTACAACCATTTGTA+AGG 0.396343 3_1:+19773028 Msa0352070:CDS
GGTTTATATCCCAAACCATT+TGG 0.399353 3_1:-19770674 None:intergenic
TGTAAGGAAGGCTTCTCTAA+TGG 0.399798 3_1:+19773044 Msa0352070:CDS
ATAACATTGACTTGCAAAAC+AGG 0.403737 3_1:-19770860 None:intergenic
TTTATACAAAGTGAATGTAT+AGG 0.415577 3_1:+19774030 Msa0352070:three_prime_UTR
CTTGTCATTATATGTGTTGA+TGG 0.416358 3_1:-19771311 None:intergenic
GTGAGGACAGGTTGTCTCCC+TGG 0.417494 3_1:+19772397 Msa0352070:CDS
GAACTGCATCAAGACTCATT+AGG 0.418878 3_1:+19773428 Msa0352070:CDS
ACACGTAGTCACCAAAATAA+AGG 0.427153 3_1:-19773785 None:intergenic
CATGTGCAACTTCAAGCAAA+AGG 0.428252 3_1:+19770629 Msa0352070:CDS
AACTGCATCAAGACTCATTA+GGG 0.430097 3_1:+19773429 Msa0352070:CDS
TTGTTACCAAGTTCCTCAAC+TGG 0.432339 3_1:-19772304 None:intergenic
TTTGAGGAAATGGGGTGTCA+GGG 0.436312 3_1:-19770433 None:intergenic
TCTCTGAAGAAGAGTTCAAC+AGG 0.437881 3_1:+19773396 Msa0352070:CDS
AGAGAAGCCTTCCTTACAAA+TGG 0.441636 3_1:-19773039 None:intergenic
GGATAGAAGAAAGTATCTTA+TGG 0.445638 3_1:+19770748 Msa0352070:CDS
ACAAGAACAAGAACAGGAAC+AGG 0.454012 3_1:+19770577 Msa0352070:CDS
TGAATGTTATCGAAGTTATG+AGG 0.455620 3_1:+19772536 Msa0352070:CDS
CTTGTGTTAGTGTGTAAATC+AGG 0.456028 3_1:-19773543 None:intergenic
GCTGAGCCAGTTGAGGAACT+TGG 0.456348 3_1:+19772298 Msa0352070:CDS
TCTTCATTTAAGAATGGAAA+AGG 0.458460 3_1:+19772366 Msa0352070:CDS
ATATCTTGCACGGATCCAAC+CGG 0.459748 3_1:-19771369 None:intergenic
TGAATTTCTTGTTCAGTGGT+AGG 0.463487 3_1:-19773587 None:intergenic
GCACTCAGGGACAGGATACT+TGG 0.464829 3_1:-19773492 None:intergenic
CTTATGGTTGTTGTTTCTGG+TGG 0.465215 3_1:+19770764 Msa0352070:CDS
TTCATTATTATGAATACTTG+TGG 0.467258 3_1:+19774088 Msa0352070:three_prime_UTR
TGGGCTGGAGGGAAACCATT+GGG 0.467264 3_1:+19772931 Msa0352070:CDS
ACGTGTCACTGTCGTGTGTT+TGG 0.467810 3_1:+19773866 Msa0352070:three_prime_UTR
AAGGATGTTTGGGTGAGGAC+AGG 0.469149 3_1:+19772385 Msa0352070:CDS
AAGGAAGATCTTGCCTTGCC+AGG 0.480516 3_1:+19773006 Msa0352070:CDS
TTCTGAAGATTTGTGGAGCA+TGG 0.482167 3_1:-19770458 None:intergenic
ATTACAACCATTTGTAAGGA+AGG 0.482613 3_1:+19773032 Msa0352070:CDS
CAAAGACCAGAGCTCTTGAC+TGG 0.483694 3_1:+19772463 Msa0352070:CDS
ATAAGTGTTGATGCCGGTCT+CGG 0.484933 3_1:-19773826 None:intergenic
TTTGGAACAAGAACAAGAAC+AGG 0.487399 3_1:+19770571 Msa0352070:CDS
ACAAATGGTTGTAATTCTCC+TGG 0.488364 3_1:-19773024 None:intergenic
GATCTCCCAGTCAAGAGCTC+TGG 0.488930 3_1:-19772469 None:intergenic
TTATGGTTGTTGTTTCTGGT+GGG 0.490217 3_1:+19770765 Msa0352070:CDS
CCATCTCATGGAGGAAATAT+GGG 0.491947 3_1:+19773111 Msa0352070:CDS
GTCGGTGCTTCAGGGTCATA+GGG 0.514514 3_1:+19773301 Msa0352070:intron
ATGTTATTTGGGGTGATGAA+AGG 0.517325 3_1:+19770876 Msa0352070:CDS
AGCACTTCAATTTCCTTCCA+GGG 0.518471 3_1:+19773653 Msa0352070:three_prime_UTR
GAATTTCTTGTTCAGTGGTA+GGG 0.519084 3_1:-19773586 None:intergenic
TTGGGCTGGAGGGAAACCAT+TGG 0.519413 3_1:+19772930 Msa0352070:CDS
ATGTGCAACTTCAAGCAAAA+GGG 0.525666 3_1:+19770630 Msa0352070:CDS
TCACATTCTCTGAAACTCAA+TGG 0.529041 3_1:+19770205 Msa0352070:exon
CACTTCATGCTACTCCCGGT+TGG 0.529254 3_1:+19771354 Msa0352070:CDS
ATTGAGTTTATGATTTGTGA+TGG 0.529898 3_1:-19770272 None:intergenic
TTTAGGTTACTGAAAGCTCT+AGG 0.530891 3_1:+19772883 Msa0352070:intron
CAGGAACAGGAACAAGAAAG+AGG 0.531355 3_1:+19770590 Msa0352070:CDS
AGTGGAGGCCTTCCTTCCAC+TGG 0.535806 3_1:-19771335 None:intergenic
ATGAAGCATTGACACAAATA+CGG 0.535823 3_1:-19773892 None:intergenic
TTGCACATGCACTCAGGGAC+AGG 0.536508 3_1:-19773500 None:intergenic
CTTGGACTCGAGGCTTTCAA+AGG 0.536650 3_1:+19771890 Msa0352070:CDS
TTAATAACCTCGTCGTACTC+AGG 0.537386 3_1:-19772427 None:intergenic
TTATAGGAGACAAAGTGAAA+AGG 0.548920 3_1:+19770718 Msa0352070:CDS
TCTTCAGAAGCTTCGTTGCA+AGG 0.550451 3_1:+19771926 Msa0352070:CDS
AGAGTTCAACAGGATTATCA+AGG 0.552469 3_1:+19773406 Msa0352070:CDS
CCTCCACTTCATGCTACTCC+CGG 0.552569 3_1:+19771350 Msa0352070:CDS
ATTAATGGGAGTACACCTGC+AGG 0.553786 3_1:-19773943 None:intergenic
CGAAGCTTCTGAAGATCAGA+AGG 0.555068 3_1:-19771918 None:intergenic
TTGATGCTGTTGTTATTGCT+AGG 0.555554 3_1:+19770813 Msa0352070:CDS
CTCTTCTGAGATATCTTGCA+CGG 0.560668 3_1:-19771379 None:intergenic
ATAACTTCGATAACATTCAA+AGG 0.562246 3_1:-19772532 None:intergenic
TGGAAAAGGATGTTTGGGTG+AGG 0.564526 3_1:+19772380 Msa0352070:CDS
CCCATATTTCCTCCATGAGA+TGG 0.564743 3_1:-19773111 None:intergenic
TCATCATTGCACATGCACTC+AGG 0.566648 3_1:-19773506 None:intergenic
CTTAAATGAAGAGCAAGGTA+AGG 0.569568 3_1:-19772355 None:intergenic
GAAATTGAAGTGCTTCCAAA+GGG 0.572256 3_1:-19773644 None:intergenic
TGACAAGACCAGTGGAAGGA+AGG 0.573045 3_1:+19771327 Msa0352070:CDS
TCATGTCTAGTGTCCGAGAC+CGG 0.575431 3_1:+19773813 Msa0352070:three_prime_UTR
AAGATTTGCTGAGCCAGTTG+AGG 0.575476 3_1:+19772291 Msa0352070:CDS
TACCGGTGTCATGTCCGTCT+CGG 0.579238 3_1:-19773742 None:intergenic
GGAAATTGAAGTGCTTCCAA+AGG 0.583777 3_1:-19773645 None:intergenic
CATCATTGCACATGCACTCA+GGG 0.585058 3_1:-19773505 None:intergenic
GCAGAAAGAATGAAAAGCAA+AGG 0.585126 3_1:+19772331 Msa0352070:CDS
CACTTGAATTTCTTGTTCAG+TGG 0.585738 3_1:-19773591 None:intergenic
CCATTCTTAAATGAAGAGCA+AGG 0.589706 3_1:-19772360 None:intergenic
CCACTCTCACATCATTTGCA+AGG 0.590073 3_1:-19771277 None:intergenic
CATTAGGGCAGCCAGAGCTA+AGG 0.591137 3_1:+19773444 Msa0352070:CDS
TAGAAGTGTCGATGCTACAT+AGG 0.593043 3_1:-19773699 None:intergenic
GGGATGTATGTGTAAAAGAA+TGG 0.596514 3_1:-19773922 None:intergenic
TGGTTGTAATTCTCCTGGCA+AGG 0.596530 3_1:-19773019 None:intergenic
CAACCGGGAGTAGCATGAAG+TGG 0.597704 3_1:-19771353 None:intergenic
TCGTACTCAGGACTTAGACC+AGG 0.598775 3_1:-19772415 None:intergenic
ACAATTATAAGTGTTGATGC+CGG 0.603765 3_1:-19773832 None:intergenic
GCACTTCAATTTCCTTCCAG+GGG 0.606283 3_1:+19773654 Msa0352070:three_prime_UTR
ACAATCATACCTGAATTGCA+CGG 0.606633 3_1:-19773134 None:intergenic
TTAGATGTAATAAAATGTAC+CGG 0.612293 3_1:-19773759 None:intergenic
TATCTTGCACGGATCCAACC+GGG 0.613488 3_1:-19771368 None:intergenic
CACTTCAATTTCCTTCCAGG+GGG 0.614929 3_1:+19773655 Msa0352070:three_prime_UTR
GACATGTTTGGTGTCCGAGA+CGG 0.620081 3_1:+19773728 Msa0352070:three_prime_UTR
ACTATGCAGTTCAATAGAGA+AGG 0.620483 3_1:+19771849 Msa0352070:intron
AAAGACCAGAGCTCTTGACT+GGG 0.628650 3_1:+19772464 Msa0352070:CDS
TGTTAGTGTGTAAATCAGGA+TGG 0.637167 3_1:-19773539 None:intergenic
CCTTGCAAATGATGTGAGAG+TGG 0.638538 3_1:+19771277 Msa0352070:CDS
CGTACTCAGGACTTAGACCA+GGG 0.645275 3_1:-19772414 None:intergenic
TCATTACCAAAACTCAAACA+AGG 0.655909 3_1:-19770695 None:intergenic
CCGGGAGTAGCATGAAGTGG+AGG 0.662229 3_1:-19771350 None:intergenic
TTGCAAGTCAATGTTATTTG+GGG 0.663997 3_1:+19770866 Msa0352070:CDS
GTCCGAGACGGACATGACAC+CGG 0.665091 3_1:+19773740 Msa0352070:three_prime_UTR
ACATATAATGACAAGACCAG+TGG 0.677823 3_1:+19771319 Msa0352070:CDS
ATAATGACAAGACCAGTGGA+AGG 0.679368 3_1:+19771323 Msa0352070:CDS
TTAATGGGAGTACACCTGCA+GGG 0.695520 3_1:-19773942 None:intergenic
CTGGTGGGATGAATCAACAG+AGG 0.721624 3_1:+19770780 Msa0352070:CDS
GCTGGAGGGAAACCATTGGG+TGG 0.722830 3_1:+19772934 Msa0352070:CDS
TCTAAGTCCTGAGTACGACG+AGG 0.769271 3_1:+19772420 Msa0352070:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCATATATAATGAGTAAAA+GGG - chr3_1:19771818-19771837 None:intergenic 15.0%
!! TATCATATATAATGAGTAAA+AGG - chr3_1:19771819-19771838 None:intergenic 15.0%
!!! TGTTTTTCTCAAATTATTAT+AGG + chr3_1:19772726-19772745 Msa0352070:intron 15.0%
!! AAAAGATGCAAAAATTTATT+GGG + chr3_1:19772912-19772931 Msa0352070:CDS 15.0%
!!! ACATTTTAGAAAATTATTAC+TGG + chr3_1:19773234-19773253 Msa0352070:intron 15.0%
!! ATGTAATTGTATGACTTTAA+TGG + chr3_1:19771164-19771183 Msa0352070:intron 20.0%
!! TCTCAAATTATTGTTAATCT+CGG - chr3_1:19771545-19771564 None:intergenic 20.0%
!! TTGATTGTAACAATATGTTT+CGG + chr3_1:19771576-19771595 Msa0352070:intron 20.0%
!!! AAAGTTTCTTCTTTTCTTTA+TGG + chr3_1:19771955-19771974 Msa0352070:intron 20.0%
!!! ATGTTTTTGGTTTCATATTT+AGG + chr3_1:19772866-19772885 Msa0352070:intron 20.0%
!!! CAATAAATTTTTGCATCTTT+TGG - chr3_1:19772913-19772932 None:intergenic 20.0%
!! CAAAAGATGCAAAAATTTAT+TGG + chr3_1:19772911-19772930 Msa0352070:CDS 20.0%
!!! GTTTCCTAATTTTTACAATA+AGG + chr3_1:19772987-19773006 Msa0352070:CDS 20.0%
!! TCTTCCTTATTGTAAAAATT+AGG - chr3_1:19772994-19773013 None:intergenic 20.0%
!! AGTGAAGAATTTAGAAAATA+AGG - chr3_1:19773377-19773396 None:intergenic 20.0%
!!! AAGAAATTCAAGTGTTATTT+TGG + chr3_1:19773600-19773619 Msa0352070:three_prime_UTR 20.0%
!! TTAGATGTAATAAAATGTAC+CGG - chr3_1:19773762-19773781 None:intergenic 20.0%
!! TCAACACTTATAATTGTTAT+TGG + chr3_1:19773838-19773857 Msa0352070:three_prime_UTR 20.0%
!! TTTATACAAAGTGAATGTAT+AGG + chr3_1:19774030-19774049 Msa0352070:three_prime_UTR 20.0%
!! TTCATTATTATGAATACTTG+TGG + chr3_1:19774088-19774107 Msa0352070:three_prime_UTR 20.0%
!! ATTGAGTTTATGATTTGTGA+TGG - chr3_1:19770275-19770294 None:intergenic 25.0%
!!! TATAAACCTTGTTTGAGTTT+TGG + chr3_1:19770689-19770708 Msa0352070:CDS 25.0%
!!! TGAGTTTTGGTAATGATTAT+AGG + chr3_1:19770702-19770721 Msa0352070:CDS 25.0%
!! TTTTGCAAGTCAATGTTATT+TGG + chr3_1:19770864-19770883 Msa0352070:CDS 25.0%
!! TTTGCAAGTCAATGTTATTT+GGG + chr3_1:19770865-19770884 Msa0352070:CDS 25.0%
! TTGTCTGAAAATGACATATA+TGG - chr3_1:19771491-19771510 None:intergenic 25.0%
!!! ATATATGTCATTTTCAGACA+AGG + chr3_1:19771490-19771509 Msa0352070:intron 25.0%
! ACCGAAATTAATATAATCAG+AGG + chr3_1:19771672-19771691 Msa0352070:intron 25.0%
! GCCTCTGATTATATTAATTT+CGG - chr3_1:19771676-19771695 None:intergenic 25.0%
! TGCATAATATTCATCTTGTT+TGG + chr3_1:19771758-19771777 Msa0352070:intron 25.0%
!!! GATTTTGAGAAATTGAGTTA+GGG - chr3_1:19772069-19772088 None:intergenic 25.0%
! CTAACTCAATTTCTCAAAAT+CGG + chr3_1:19772068-19772087 Msa0352070:intron 25.0%
! TCTTCATTTAAGAATGGAAA+AGG + chr3_1:19772366-19772385 Msa0352070:CDS 25.0%
! ATAACTTCGATAACATTCAA+AGG - chr3_1:19772535-19772554 None:intergenic 25.0%
! ATAAGTGACCTATTGTAAAA+GGG - chr3_1:19772624-19772643 None:intergenic 25.0%
!! GATTTCATGATAGATGTTTT+TGG + chr3_1:19772853-19772872 Msa0352070:intron 25.0%
! TGTAAAAATTAGGAAACTCT+TGG - chr3_1:19772984-19773003 None:intergenic 25.0%
!!! AATTCAAGTGTTATTTTGGA+TGG + chr3_1:19773604-19773623 Msa0352070:three_prime_UTR 25.0%
!!! ACATCTAATCACCTTTATTT+TGG + chr3_1:19773774-19773793 Msa0352070:three_prime_UTR 25.0%
! CAACCTAACTAAGTATTAAT+GGG - chr3_1:19773960-19773979 None:intergenic 25.0%
! TCAACCTAACTAAGTATTAA+TGG - chr3_1:19773961-19773980 None:intergenic 25.0%
TAACTTCTTTGCATTGTTCT+TGG - chr3_1:19770237-19770256 None:intergenic 30.0%
!! GTAGTTTTTGATGACTTCTT+TGG - chr3_1:19770332-19770351 None:intergenic 30.0%
!!! TGTTGATTTTCTTGTGAAGA+TGG - chr3_1:19770354-19770373 None:intergenic 30.0%
TCACAAGAAAATCAACACTA+TGG + chr3_1:19770357-19770376 Msa0352070:CDS 30.0%
! TTCTTTGGTATGTTCAAGTT+TGG + chr3_1:19770404-19770423 Msa0352070:CDS 30.0%
! CATCATTCATTTTCCAAACT+TGG + chr3_1:19770497-19770516 Msa0352070:CDS 30.0%
GTAGCAAAAACTCATCATTA+TGG - chr3_1:19770544-19770563 None:intergenic 30.0%
! AATGATGAGTTTTTGCTACA+TGG + chr3_1:19770545-19770564 Msa0352070:CDS 30.0%
TCATTACCAAAACTCAAACA+AGG - chr3_1:19770698-19770717 None:intergenic 30.0%
TTATAGGAGACAAAGTGAAA+AGG + chr3_1:19770718-19770737 Msa0352070:CDS 30.0%
!! ACAAAGTGAAAAGGTTTTGA+AGG + chr3_1:19770727-19770746 Msa0352070:CDS 30.0%
!! GGATAGAAGAAAGTATCTTA+TGG + chr3_1:19770748-19770767 Msa0352070:CDS 30.0%
!! TATCTTATGGTTGTTGTTTC+TGG + chr3_1:19770761-19770780 Msa0352070:CDS 30.0%
!! ATAACATTGACTTGCAAAAC+AGG - chr3_1:19770863-19770882 None:intergenic 30.0%
! TTGCAAGTCAATGTTATTTG+GGG + chr3_1:19770866-19770885 Msa0352070:CDS 30.0%
! AATGTTTCTGTTCATGTAAG+TGG + chr3_1:19771109-19771128 Msa0352070:intron 30.0%
TGCTTTGATTGTAGTGAATT+TGG + chr3_1:19771221-19771240 Msa0352070:intron 30.0%
CTTGTCATTATATGTGTTGA+TGG - chr3_1:19771314-19771333 None:intergenic 30.0%
!! AAGAATAGTTCACTGCTTTT+TGG + chr3_1:19771403-19771422 Msa0352070:intron 30.0%
ATTCCACAAATGTAGATTGT+AGG - chr3_1:19771464-19771483 None:intergenic 30.0%
! CATCTTGTTTGGAAAATGTA+AGG + chr3_1:19771769-19771788 Msa0352070:intron 30.0%
! ATGCTATTTGTCAAAATACG+AGG + chr3_1:19771999-19772018 Msa0352070:intron 30.0%
!! CGATTTTGAGAAATTGAGTT+AGG - chr3_1:19772070-19772089 None:intergenic 30.0%
! AGTCTTTGTAAGTATTTTCG+TGG - chr3_1:19772193-19772212 None:intergenic 30.0%
! AAGTTGTCGTCTAAATTTTC+AGG + chr3_1:19772252-19772271 Msa0352070:intron 30.0%
TAAGAATGGAAAAGGATGTT+TGG + chr3_1:19772374-19772393 Msa0352070:CDS 30.0%
AAGAATGGAAAAGGATGTTT+GGG + chr3_1:19772375-19772394 Msa0352070:CDS 30.0%
GACTTACCATCAAAGAAAAA+TGG + chr3_1:19772498-19772517 Msa0352070:CDS 30.0%
TGAATGTTATCGAAGTTATG+AGG + chr3_1:19772536-19772555 Msa0352070:CDS 30.0%
TTGTAAAAGGGCTACTAAAT+AGG - chr3_1:19772612-19772631 None:intergenic 30.0%
! TTTAGTAGCCCTTTTACAAT+AGG + chr3_1:19772613-19772632 Msa0352070:intron 30.0%
CATAAGTGACCTATTGTAAA+AGG - chr3_1:19772625-19772644 None:intergenic 30.0%
CTATATTCTAATAGCAGACT+TGG - chr3_1:19772806-19772825 None:intergenic 30.0%
TTAGGAAACTCTTGGATTAA+TGG - chr3_1:19772976-19772995 None:intergenic 30.0%
GAGAATTACAACCATTTGTA+AGG + chr3_1:19773028-19773047 Msa0352070:CDS 30.0%
ATTACAACCATTTGTAAGGA+AGG + chr3_1:19773032-19773051 Msa0352070:CDS 30.0%
AGCTAAGGACTAACAAAAAT+GGG + chr3_1:19773459-19773478 Msa0352070:CDS 30.0%
CTTCTAATTGAAGACATGTT+TGG + chr3_1:19773716-19773735 Msa0352070:three_prime_UTR 30.0%
!! ACAATTATAAGTGTTGATGC+CGG - chr3_1:19773835-19773854 None:intergenic 30.0%
!! ATGAAGCATTGACACAAATA+CGG - chr3_1:19773895-19773914 None:intergenic 30.0%
ACTCCCATTAATACTTAGTT+AGG + chr3_1:19773954-19773973 Msa0352070:three_prime_UTR 30.0%
!! TCACATTCTCTGAAACTCAA+TGG + chr3_1:19770205-19770224 Msa0352070:exon 35.0%
! TTTCTTGCTCTGTTTCTTGT+TGG + chr3_1:19770380-19770399 Msa0352070:CDS 35.0%
!! CTGTTTCTTGTTGGTTTCTT+TGG + chr3_1:19770389-19770408 Msa0352070:CDS 35.0%
!! AGCATGGTTTTTGAGGAAAT+GGG - chr3_1:19770445-19770464 None:intergenic 35.0%
! TGAGAACTTCTGAAGATTTG+TGG - chr3_1:19770468-19770487 None:intergenic 35.0%
CAGAATACTAAGACCAAGTT+TGG - chr3_1:19770513-19770532 None:intergenic 35.0%
! GTTTTTGCTACATGGTAGTT+TGG + chr3_1:19770553-19770572 Msa0352070:CDS 35.0%
! TTTGGAACAAGAACAAGAAC+AGG + chr3_1:19770571-19770590 Msa0352070:CDS 35.0%
! ACAAGAAAGAGGACAGTTTT+TGG + chr3_1:19770601-19770620 Msa0352070:CDS 35.0%
ATGTGCAACTTCAAGCAAAA+GGG + chr3_1:19770630-19770649 Msa0352070:CDS 35.0%
!!! GAGTTTTGGAAGAAACCAAA+TGG + chr3_1:19770659-19770678 Msa0352070:CDS 35.0%
TTGGAAGAAACCAAATGGTT+TGG + chr3_1:19770664-19770683 Msa0352070:CDS 35.0%
TGGAAGAAACCAAATGGTTT+GGG + chr3_1:19770665-19770684 Msa0352070:CDS 35.0%
GGTTTATATCCCAAACCATT+TGG - chr3_1:19770677-19770696 None:intergenic 35.0%
!! TTATGGTTGTTGTTTCTGGT+GGG + chr3_1:19770765-19770784 Msa0352070:CDS 35.0%
! TTGATGCTGTTGTTATTGCT+AGG + chr3_1:19770813-19770832 Msa0352070:CDS 35.0%
! GTTGTTATTGCTAGGATTCT+TGG + chr3_1:19770821-19770840 Msa0352070:CDS 35.0%
! ATGTTATTTGGGGTGATGAA+AGG + chr3_1:19770876-19770895 Msa0352070:CDS 35.0%
ACATATAATGACAAGACCAG+TGG + chr3_1:19771319-19771338 Msa0352070:CDS 35.0%
GGACTCAAAATTATTCACAC+AGG - chr3_1:19771443-19771462 None:intergenic 35.0%
AGTCCTACAATCTACATTTG+TGG + chr3_1:19771458-19771477 Msa0352070:intron 35.0%
! TCATTTTCAGACAAGGTCAT+CGG + chr3_1:19771497-19771516 Msa0352070:intron 35.0%
! ATCTGAAGTGTTCGTTACAT+AGG + chr3_1:19771634-19771653 Msa0352070:intron 35.0%
CAACAAATTGGTAGGTACAA+GGG - chr3_1:19771701-19771720 None:intergenic 35.0%
AACAATTGCCTGCAACAAAT+TGG - chr3_1:19771713-19771732 None:intergenic 35.0%
TTGAACTGCATAGTACAACA+AGG - chr3_1:19771843-19771862 None:intergenic 35.0%
ACTATGCAGTTCAATAGAGA+AGG + chr3_1:19771849-19771868 Msa0352070:intron 35.0%
TGCGTTCGTGAAAATAAGTT+AGG + chr3_1:19772043-19772062 Msa0352070:intron 35.0%
TCGTATAAGACCATATCATC+AGG + chr3_1:19772135-19772154 Msa0352070:intron 35.0%
TTTCACAATCTGCAATCACT+TGG - chr3_1:19772234-19772253 None:intergenic 35.0%
GCAGAAAGAATGAAAAGCAA+AGG + chr3_1:19772331-19772350 Msa0352070:CDS 35.0%
CTTAAATGAAGAGCAAGGTA+AGG - chr3_1:19772358-19772377 None:intergenic 35.0%
CCATTCTTAAATGAAGAGCA+AGG - chr3_1:19772363-19772382 None:intergenic 35.0%
CCTTGCTCTTCATTTAAGAA+TGG + chr3_1:19772360-19772379 Msa0352070:CDS 35.0%
TACCATCAAAGAAAAATGGC+TGG + chr3_1:19772502-19772521 Msa0352070:CDS 35.0%
! TTTAGGTTACTGAAAGCTCT+AGG + chr3_1:19772883-19772902 Msa0352070:intron 35.0%
GATGCAAAAATTTATTGGGC+TGG + chr3_1:19772916-19772935 Msa0352070:CDS 35.0%
! CAAAAATTTATTGGGCTGGA+GGG + chr3_1:19772920-19772939 Msa0352070:CDS 35.0%
ACAAATGGTTGTAATTCTCC+TGG - chr3_1:19773027-19773046 None:intergenic 35.0%
CTCAGAGACTATGTAATCTA+TGG - chr3_1:19773073-19773092 None:intergenic 35.0%
ACAATCATACCTGAATTGCA+CGG - chr3_1:19773137-19773156 None:intergenic 35.0%
TATGATTGTCAAGTTCGACA+AGG + chr3_1:19773148-19773167 Msa0352070:intron 35.0%
ATGATTGTCAAGTTCGACAA+GGG + chr3_1:19773149-19773168 Msa0352070:intron 35.0%
AGAGTTCAACAGGATTATCA+AGG + chr3_1:19773406-19773425 Msa0352070:CDS 35.0%
AACTGCATCAAGACTCATTA+GGG + chr3_1:19773429-19773448 Msa0352070:CDS 35.0%
! TTTTTGTTAGTCCTTAGCTC+TGG - chr3_1:19773458-19773477 None:intergenic 35.0%
GAGCTAAGGACTAACAAAAA+TGG + chr3_1:19773458-19773477 Msa0352070:CDS 35.0%
TGTTAGTGTGTAAATCAGGA+TGG - chr3_1:19773542-19773561 None:intergenic 35.0%
CTTGTGTTAGTGTGTAAATC+AGG - chr3_1:19773546-19773565 None:intergenic 35.0%
TACACACTAACACAAGATTC+TGG + chr3_1:19773550-19773569 Msa0352070:three_prime_UTR 35.0%
!! CTAACACAAGATTCTGGTTT+AGG + chr3_1:19773556-19773575 Msa0352070:three_prime_UTR 35.0%
GAATTTCTTGTTCAGTGGTA+GGG - chr3_1:19773589-19773608 None:intergenic 35.0%
TGAATTTCTTGTTCAGTGGT+AGG - chr3_1:19773590-19773609 None:intergenic 35.0%
CACTTGAATTTCTTGTTCAG+TGG - chr3_1:19773594-19773613 None:intergenic 35.0%
CTTCCAAAGGGATCAAAAAA+TGG - chr3_1:19773635-19773654 None:intergenic 35.0%
! GAAATTGAAGTGCTTCCAAA+GGG - chr3_1:19773647-19773666 None:intergenic 35.0%
ACACGTAGTCACCAAAATAA+AGG - chr3_1:19773788-19773807 None:intergenic 35.0%
GGGATGTATGTGTAAAAGAA+TGG - chr3_1:19773925-19773944 None:intergenic 35.0%
!! GCATGGTTTTTGAGGAAATG+GGG - chr3_1:19770444-19770463 None:intergenic 40.0%
!! GAGCATGGTTTTTGAGGAAA+TGG - chr3_1:19770446-19770465 None:intergenic 40.0%
! TTTGTGGAGCATGGTTTTTG+AGG - chr3_1:19770452-19770471 None:intergenic 40.0%
! TTCTGAAGATTTGTGGAGCA+TGG - chr3_1:19770461-19770480 None:intergenic 40.0%
ACAAGAACAAGAACAGGAAC+AGG + chr3_1:19770577-19770596 Msa0352070:CDS 40.0%
CATGTGCAACTTCAAGCAAA+AGG + chr3_1:19770629-19770648 Msa0352070:CDS 40.0%
! CAAAAGGGTCTAGTGAGTTT+TGG + chr3_1:19770645-19770664 Msa0352070:CDS 40.0%
!! CTTATGGTTGTTGTTTCTGG+TGG + chr3_1:19770764-19770783 Msa0352070:CDS 40.0%
!! TAGGATTCTTGGTGCTTCTT+TGG + chr3_1:19770832-19770851 Msa0352070:CDS 40.0%
! GCACTGATACTTCACATTGA+AGG + chr3_1:19770974-19770993 Msa0352070:intron 40.0%
!! TGGTGGTGTCTAACATGTTT+TGG + chr3_1:19771003-19771022 Msa0352070:intron 40.0%
ATAATGACAAGACCAGTGGA+AGG + chr3_1:19771323-19771342 Msa0352070:CDS 40.0%
!! CTCTTCTGAGATATCTTGCA+CGG - chr3_1:19771382-19771401 None:intergenic 40.0%
CTTCAGATTGAAGACACGTT+TGG - chr3_1:19771622-19771641 None:intergenic 40.0%
GCAACAAATTGGTAGGTACA+AGG - chr3_1:19771702-19771721 None:intergenic 40.0%
!! TGTACCTACCAATTTGTTGC+AGG + chr3_1:19771702-19771721 Msa0352070:intron 40.0%
ATTGCCTGCAACAAATTGGT+AGG - chr3_1:19771709-19771728 None:intergenic 40.0%
!!! GAAGGTGTTTTGCTTTTACG+TGG + chr3_1:19771867-19771886 Msa0352070:CDS 40.0%
!! TGTTTTGCTTTTACGTGGCT+TGG + chr3_1:19771872-19771891 Msa0352070:CDS 40.0%
TCTCACATCACCTGATGATA+TGG - chr3_1:19772148-19772167 None:intergenic 40.0%
! ATTTTCGTGGCTTTTCTTCG+CGG - chr3_1:19772180-19772199 None:intergenic 40.0%
TTGTTACCAAGTTCCTCAAC+TGG - chr3_1:19772307-19772326 None:intergenic 40.0%
TTAATAACCTCGTCGTACTC+AGG - chr3_1:19772430-19772449 None:intergenic 40.0%
!!! GTCCAGCCATTTTTCTTTGA+TGG - chr3_1:19772507-19772526 None:intergenic 40.0%
TTGGACATGAAGCACAAACA+CGG - chr3_1:19772787-19772806 None:intergenic 40.0%
! GCAAAAATTTATTGGGCTGG+AGG + chr3_1:19772919-19772938 Msa0352070:CDS 40.0%
! AAAGCTTCTTTTCCACCCAA+TGG - chr3_1:19772949-19772968 None:intergenic 40.0%
AGAGAAGCCTTCCTTACAAA+TGG - chr3_1:19773042-19773061 None:intergenic 40.0%
TGTAAGGAAGGCTTCTCTAA+TGG + chr3_1:19773044-19773063 Msa0352070:CDS 40.0%
! GATGTTTTCATGCCATCTCA+TGG + chr3_1:19773099-19773118 Msa0352070:CDS 40.0%
CCATCTCATGGAGGAAATAT+GGG + chr3_1:19773111-19773130 Msa0352070:CDS 40.0%
AGATACGTACTAAGACGAGT+TGG - chr3_1:19773188-19773207 None:intergenic 40.0%
GTCTGATGTTTGTGTCTTGT+CGG + chr3_1:19773283-19773302 Msa0352070:intron 40.0%
! TCTCTGAAGAAGAGTTCAAC+AGG + chr3_1:19773396-19773415 Msa0352070:CDS 40.0%
GAACTGCATCAAGACTCATT+AGG + chr3_1:19773428-19773447 Msa0352070:CDS 40.0%
!!! GCTCCATTTTTTGATCCCTT+TGG + chr3_1:19773629-19773648 Msa0352070:three_prime_UTR 40.0%
! GGAAATTGAAGTGCTTCCAA+AGG - chr3_1:19773648-19773667 None:intergenic 40.0%
AAGCACTTCAATTTCCTTCC+AGG + chr3_1:19773652-19773671 Msa0352070:three_prime_UTR 40.0%
AGCACTTCAATTTCCTTCCA+GGG + chr3_1:19773653-19773672 Msa0352070:three_prime_UTR 40.0%
!! TAGAAGTGTCGATGCTACAT+AGG - chr3_1:19773702-19773721 None:intergenic 40.0%
! TTTTACACATACATCCCTGC+AGG + chr3_1:19773928-19773947 Msa0352070:three_prime_UTR 40.0%
! TTTGAGGAAATGGGGTGTCA+GGG - chr3_1:19770436-19770455 None:intergenic 45.0%
!! TTTTGAGGAAATGGGGTGTC+AGG - chr3_1:19770437-19770456 None:intergenic 45.0%
CAGGAACAGGAACAAGAAAG+AGG + chr3_1:19770590-19770609 Msa0352070:CDS 45.0%
! CTTCACATTGAAGGCGTGTT+TGG + chr3_1:19770983-19771002 Msa0352070:intron 45.0%
CCACTCTCACATCATTTGCA+AGG - chr3_1:19771280-19771299 None:intergenic 45.0%
CCTTGCAAATGATGTGAGAG+TGG + chr3_1:19771277-19771296 Msa0352070:CDS 45.0%
ATATCTTGCACGGATCCAAC+CGG - chr3_1:19771372-19771391 None:intergenic 45.0%
! CGAAGCTTCTGAAGATCAGA+AGG - chr3_1:19771921-19771940 None:intergenic 45.0%
TCTTCAGAAGCTTCGTTGCA+AGG + chr3_1:19771926-19771945 Msa0352070:CDS 45.0%
!! AAGATTTGCTGAGCCAGTTG+AGG + chr3_1:19772291-19772310 Msa0352070:CDS 45.0%
TGGAAAAGGATGTTTGGGTG+AGG + chr3_1:19772380-19772399 Msa0352070:CDS 45.0%
AAAGACCAGAGCTCTTGACT+GGG + chr3_1:19772464-19772483 Msa0352070:CDS 45.0%
TGGTTGTAATTCTCCTGGCA+AGG - chr3_1:19773022-19773041 None:intergenic 45.0%
! GTTTTCATGCCATCTCATGG+AGG + chr3_1:19773102-19773121 Msa0352070:CDS 45.0%
GCCATCTCATGGAGGAAATA+TGG + chr3_1:19773110-19773129 Msa0352070:CDS 45.0%
CCCATATTTCCTCCATGAGA+TGG - chr3_1:19773114-19773133 None:intergenic 45.0%
AAATATGGGCCGTGCAATTC+AGG + chr3_1:19773125-19773144 Msa0352070:CDS 45.0%
! GGAAGCATGTGTCTCTTGTT+TGG - chr3_1:19773356-19773375 None:intergenic 45.0%
CATCATTGCACATGCACTCA+GGG - chr3_1:19773508-19773527 None:intergenic 45.0%
TCATCATTGCACATGCACTC+AGG - chr3_1:19773509-19773528 None:intergenic 45.0%
GCACTTCAATTTCCTTCCAG+GGG + chr3_1:19773654-19773673 Msa0352070:three_prime_UTR 45.0%
CACTTCAATTTCCTTCCAGG+GGG + chr3_1:19773655-19773674 Msa0352070:three_prime_UTR 45.0%
TCTTATAGTTTCCCCCTGGA+AGG - chr3_1:19773669-19773688 None:intergenic 45.0%
GAACTCTTATAGTTTCCCCC+TGG - chr3_1:19773673-19773692 None:intergenic 45.0%
!! ATAAGTGTTGATGCCGGTCT+CGG - chr3_1:19773829-19773848 None:intergenic 45.0%
! TTAATGGGAGTACACCTGCA+GGG - chr3_1:19773945-19773964 None:intergenic 45.0%
! ATTAATGGGAGTACACCTGC+AGG - chr3_1:19773946-19773965 None:intergenic 45.0%
CTGGTGGGATGAATCAACAG+AGG + chr3_1:19770780-19770799 Msa0352070:CDS 50.0%
! CACATTGAAGGCGTGTTTGG+TGG + chr3_1:19770986-19771005 Msa0352070:intron 50.0%
TGACAAGACCAGTGGAAGGA+AGG + chr3_1:19771327-19771346 Msa0352070:CDS 50.0%
TATCTTGCACGGATCCAACC+GGG - chr3_1:19771371-19771390 None:intergenic 50.0%
!! TTTTACGTGGCTTGGACTCG+AGG + chr3_1:19771880-19771899 Msa0352070:CDS 50.0%
CTTGGACTCGAGGCTTTCAA+AGG + chr3_1:19771890-19771909 Msa0352070:CDS 50.0%
AAGGATGTTTGGGTGAGGAC+AGG + chr3_1:19772385-19772404 Msa0352070:CDS 50.0%
CGTACTCAGGACTTAGACCA+GGG - chr3_1:19772417-19772436 None:intergenic 50.0%
TCGTACTCAGGACTTAGACC+AGG - chr3_1:19772418-19772437 None:intergenic 50.0%
TCTAAGTCCTGAGTACGACG+AGG + chr3_1:19772420-19772439 Msa0352070:CDS 50.0%
CAAAGACCAGAGCTCTTGAC+TGG + chr3_1:19772463-19772482 Msa0352070:CDS 50.0%
!! TCACTTATGCAGCACCGATG+TGG + chr3_1:19772636-19772655 Msa0352070:intron 50.0%
!! CACTTATGCAGCACCGATGT+GGG + chr3_1:19772637-19772656 Msa0352070:intron 50.0%
CTCATGATGACCACCCACAT+CGG - chr3_1:19772653-19772672 None:intergenic 50.0%
CACGGACACATGACATGACA+TGG - chr3_1:19772769-19772788 None:intergenic 50.0%
AAGGAAGATCTTGCCTTGCC+AGG + chr3_1:19773006-19773025 Msa0352070:CDS 50.0%
! TTGTGTCTTGTCGGTGCTTC+AGG + chr3_1:19773292-19773311 Msa0352070:intron 50.0%
! TGTGTCTTGTCGGTGCTTCA+GGG + chr3_1:19773293-19773312 Msa0352070:intron 50.0%
! CAGGGTCATAGGGCATTTAC+TGG + chr3_1:19773311-19773330 Msa0352070:intron 50.0%
!! GACATGTTTGGTGTCCGAGA+CGG + chr3_1:19773728-19773747 Msa0352070:three_prime_UTR 50.0%
TCATGTCTAGTGTCCGAGAC+CGG + chr3_1:19773813-19773832 Msa0352070:three_prime_UTR 50.0%
! ACGTGTCACTGTCGTGTGTT+TGG + chr3_1:19773866-19773885 Msa0352070:three_prime_UTR 50.0%
CCTCCACTTCATGCTACTCC+CGG + chr3_1:19771350-19771369 Msa0352070:CDS 55.0%
CAACCGGGAGTAGCATGAAG+TGG - chr3_1:19771356-19771375 None:intergenic 55.0%
CACTTCATGCTACTCCCGGT+TGG + chr3_1:19771354-19771373 Msa0352070:CDS 55.0%
GCTGAGCCAGTTGAGGAACT+TGG + chr3_1:19772298-19772317 Msa0352070:CDS 55.0%
GATCTCCCAGTCAAGAGCTC+TGG - chr3_1:19772472-19772491 None:intergenic 55.0%
!! TTATGCAGCACCGATGTGGG+TGG + chr3_1:19772640-19772659 Msa0352070:intron 55.0%
! TTGGGCTGGAGGGAAACCAT+TGG + chr3_1:19772930-19772949 Msa0352070:CDS 55.0%
! TGGGCTGGAGGGAAACCATT+GGG + chr3_1:19772931-19772950 Msa0352070:CDS 55.0%
! TGTCGGTGCTTCAGGGTCAT+AGG + chr3_1:19773300-19773319 Msa0352070:intron 55.0%
! GTCGGTGCTTCAGGGTCATA+GGG + chr3_1:19773301-19773320 Msa0352070:intron 55.0%
CATTAGGGCAGCCAGAGCTA+AGG + chr3_1:19773444-19773463 Msa0352070:CDS 55.0%
GCACTCAGGGACAGGATACT+TGG - chr3_1:19773495-19773514 None:intergenic 55.0%
TTGCACATGCACTCAGGGAC+AGG - chr3_1:19773503-19773522 None:intergenic 55.0%
!! TACCGGTGTCATGTCCGTCT+CGG - chr3_1:19773745-19773764 None:intergenic 55.0%
AGTGGAGGCCTTCCTTCCAC+TGG - chr3_1:19771338-19771357 None:intergenic 60.0%
CCGGGAGTAGCATGAAGTGG+AGG - chr3_1:19771353-19771372 None:intergenic 60.0%
GTGAGGACAGGTTGTCTCCC+TGG + chr3_1:19772397-19772416 Msa0352070:CDS 60.0%
GCTGGAGGGAAACCATTGGG+TGG + chr3_1:19772934-19772953 Msa0352070:CDS 60.0%
GTCCGAGACGGACATGACAC+CGG + chr3_1:19773740-19773759 Msa0352070:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 19770144 19774142 19770144 ID=Msa0352070;Name=Msa0352070
chr3_1 mRNA 19770144 19774142 19770144 ID=Msa0352070-mRNA-1;Parent=Msa0352070;Name=Msa0352070-mRNA-1;_AED=0.27;_eAED=0.27;_QI=80|1|1|1|1|1|6|595|568
chr3_1 exon 19770144 19770897 19770144 ID=Msa0352070-mRNA-1:exon:4638;Parent=Msa0352070-mRNA-1
chr3_1 exon 19771235 19771400 19771235 ID=Msa0352070-mRNA-1:exon:4639;Parent=Msa0352070-mRNA-1
chr3_1 exon 19771858 19771947 19771858 ID=Msa0352070-mRNA-1:exon:4640;Parent=Msa0352070-mRNA-1
chr3_1 exon 19772274 19772557 19772274 ID=Msa0352070-mRNA-1:exon:4641;Parent=Msa0352070-mRNA-1
chr3_1 exon 19772888 19773146 19772888 ID=Msa0352070-mRNA-1:exon:4642;Parent=Msa0352070-mRNA-1
chr3_1 exon 19773314 19774142 19773314 ID=Msa0352070-mRNA-1:exon:4643;Parent=Msa0352070-mRNA-1
chr3_1 five_prime_UTR 19770144 19770223 19770144 ID=Msa0352070-mRNA-1:five_prime_utr;Parent=Msa0352070-mRNA-1
chr3_1 CDS 19770224 19770897 19770224 ID=Msa0352070-mRNA-1:cds;Parent=Msa0352070-mRNA-1
chr3_1 CDS 19771235 19771400 19771235 ID=Msa0352070-mRNA-1:cds;Parent=Msa0352070-mRNA-1
chr3_1 CDS 19771858 19771947 19771858 ID=Msa0352070-mRNA-1:cds;Parent=Msa0352070-mRNA-1
chr3_1 CDS 19772274 19772557 19772274 ID=Msa0352070-mRNA-1:cds;Parent=Msa0352070-mRNA-1
chr3_1 CDS 19772888 19773146 19772888 ID=Msa0352070-mRNA-1:cds;Parent=Msa0352070-mRNA-1
chr3_1 CDS 19773314 19773547 19773314 ID=Msa0352070-mRNA-1:cds;Parent=Msa0352070-mRNA-1
chr3_1 three_prime_UTR 19773548 19774142 19773548 ID=Msa0352070-mRNA-1:three_prime_utr;Parent=Msa0352070-mRNA-1
Gene Sequence

>Msa0352070

ATGGCAAAATCCAAGAACAATGCAAAGAAGTTATCATACATATCAGTTCCATCACAAATCATAAACTCAATATCATCTTCTTCTCTTCAATCTCTCATTGATTCTCCAAAGAAGTCATCAAAAACTACCATCTTCACAAGAAAATCAACACTATGGTTTCTTGCTCTGTTTCTTGTTGGTTTCTTTGGTATGTTCAAGTTTGGTTTCAACCCTGACACCCCATTTCCTCAAAAACCATGCTCCACAAATCTTCAGAAGTTCTCAATATCAAATCATCATTCATTTTCCAAACTTGGTCTTAGTATTCTGAAAAATGACCATAATGATGAGTTTTTGCTACATGGTAGTTTGGAACAAGAACAAGAACAGGAACAGGAACAAGAAAGAGGACAGTTTTTGGTATCTCATGTGCAACTTCAAGCAAAAGGGTCTAGTGAGTTTTGGAAGAAACCAAATGGTTTGGGATATAAACCTTGTTTGAGTTTTGGTAATGATTATAGGAGACAAAGTGAAAAGGTTTTGAAGGATAGAAGAAAGTATCTTATGGTTGTTGTTTCTGGTGGGATGAATCAACAGAGGAATCAGATTGTTGATGCTGTTGTTATTGCTAGGATTCTTGGTGCTTCTTTGGTTGTTCCTGTTTTGCAAGTCAATGTTATTTGGGGTGATGAAAGTGAATTTGGTGACATATTTGATCTAGAACACTTCAAAAGAGTCCTTGCAAATGATGTGAGAGTGGTTTCAGCATTGCCATCAACACATATAATGACAAGACCAGTGGAAGGAAGGCCTCCACTTCATGCTACTCCCGGTTGGATCCGTGCAAGATATCTCAGAAGATTCAATAGAGAAGGTGTTTTGCTTTTACGTGGCTTGGACTCGAGGCTTTCAAAGGATCTTCCTTCTGATCTTCAGAAGCTTCGTTGCAAGGTAGCTTTTAATGCATTAAGATTTGCTGAGCCAGTTGAGGAACTTGGTAACAAAATTGCAGAAAGAATGAAAAGCAAAGGACCTTACCTTGCTCTTCATTTAAGAATGGAAAAGGATGTTTGGGTGAGGACAGGTTGTCTCCCTGGTCTAAGTCCTGAGTACGACGAGGTTATTAACAATGAGAGAATACAAAGACCAGAGCTCTTGACTGGGAGATCAAACATGACTTACCATCAAAGAAAAATGGCTGGACTTTGTCCTTTGAATGTTATCGAAGTTATGAGGTTACTGAAAGCTCTAGGAGCTCCAAAAGATGCAAAAATTTATTGGGCTGGAGGGAAACCATTGGGTGGAAAAGAAGCTTTACTTCCATTAATCCAAGAGTTTCCTAATTTTTACAATAAGGAAGATCTTGCCTTGCCAGGAGAATTACAACCATTTGTAAGGAAGGCTTCTCTAATGGCTGCCATAGATTACATAGTCTCTGAGAAGAGTGATGTTTTCATGCCATCTCATGGAGGAAATATGGGCCGTGCAATTCAGGGTCATAGGGCATTTACTGGACACAAGAAGTATATAACTCCAAACAAGAGACACATGCTTCCTTATTTTCTAAATTCTTCACTCTCTGAAGAAGAGTTCAACAGGATTATCAAGGAACTGCATCAAGACTCATTAGGGCAGCCAGAGCTAAGGACTAACAAAAATGGGAGAGATGTTACCAAGTATCCTGTCCCTGAGTGCATGTGCAATGATGACTCAAATGCTCCATCCTGA
Protein sequence

>Msa0352070

MAKSKNNAKKLSYISVPSQIINSISSSSLQSLIDSPKKSSKTTIFTRKSTLWFLALFLVGFFGMFKFGFNPDTPFPQKPCSTNLQKFSISNHHSFSKLGLSILKNDHNDEFLLHGSLEQEQEQEQEQERGQFLVSHVQLQAKGSSEFWKKPNGLGYKPCLSFGNDYRRQSEKVLKDRRKYLMVVVSGGMNQQRNQIVDAVVIARILGASLVVPVLQVNVIWGDESEFGDIFDLEHFKRVLANDVRVVSALPSTHIMTRPVEGRPPLHATPGWIRARYLRRFNREGVLLLRGLDSRLSKDLPSDLQKLRCKVAFNALRFAEPVEELGNKIAERMKSKGPYLALHLRMEKDVWVRTGCLPGLSPEYDEVINNERIQRPELLTGRSNMTYHQRKMAGLCPLNVIEVMRLLKALGAPKDAKIYWAGGKPLGGKEALLPLIQEFPNFYNKEDLALPGELQPFVRKASLMAAIDYIVSEKSDVFMPSHGGNMGRAIQGHRAFTGHKKYITPNKRHMLPYFLNSSLSEEEFNRIIKELHQDSLGQPELRTNKNGRDVTKYPVPECMCNDDSNAPS*