Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0375440 | Msa0375460 | 0.806453 | 8.165806e-50 | -8.615850e-47 |
| Msa0375450 | Msa0375460 | 0.805036 | 1.620407e-49 | -8.615850e-47 |
| Msa0375460 | Msa0375470 | 0.956194 | 5.232098e-114 | -8.615850e-47 |
| Msa0375460 | Msa0375480 | 0.806706 | 7.222857e-50 | -8.615850e-47 |
| Msa0375460 | Msa0391340 | 0.928969 | 1.359515e-92 | -8.615850e-47 |
| Msa0375460 | Msa0391350 | 0.921050 | 5.879730e-88 | -8.615850e-47 |
| Msa0375460 | Msa0391360 | 0.808396 | 3.162251e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find 122 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAATTGTGAAGAGAGAAAT+AGG | 0.224146 | 3_1:-75230108 | None:intergenic |
| TCTAAGACACCGGATCTATT+TGG | 0.254975 | 3_1:+75230237 | Msa0375460:CDS |
| ATGGGGAGTAGGAGTATTAC+TGG | 0.335430 | 3_1:+75231221 | Msa0375460:CDS |
| GGGAAGATGATCTAAGTCTA+AGG | 0.342188 | 3_1:-75230150 | None:intergenic |
| ATCTTCTAAGAGCTGCAATA+TGG | 0.364246 | 3_1:+75230202 | Msa0375460:CDS |
| GTCTAAGGGTGTGTTTGGTT+TGG | 0.384467 | 3_1:-75230135 | None:intergenic |
| ATGTCTCATGTCTGCCTCAC+TGG | 0.394407 | 3_1:+75230174 | Msa0375460:CDS |
| TCTAAGTCTAAGGGTGTGTT+TGG | 0.395921 | 3_1:-75230140 | None:intergenic |
| AAATAGATCCGGTGTCTTAG+AGG | 0.427682 | 3_1:-75230235 | None:intergenic |
| TTTAGGTAAGTATGGGGAGT+AGG | 0.443906 | 3_1:+75231210 | Msa0375460:intron |
| TCCTTTCAAGTGCTACTACA+AGG | 0.488545 | 3_1:+75230281 | Msa0375460:CDS |
| ACCTTGTAGTAGCACTTGAA+AGG | 0.540154 | 3_1:-75230282 | None:intergenic |
| TCACCGCCACCAAATAGATC+CGG | 0.557945 | 3_1:-75230246 | None:intergenic |
| ACGAGCTTCCTCTAAGACAC+CGG | 0.592995 | 3_1:+75230227 | Msa0375460:CDS |
| AAGACACCGGATCTATTTGG+TGG | 0.595439 | 3_1:+75230240 | Msa0375460:CDS |
| GGAAGATGATCTAAGTCTAA+GGG | 0.613582 | 3_1:-75230149 | None:intergenic |
| GTGAGGCAGACATGAGACAT+GGG | 0.618587 | 3_1:-75230171 | None:intergenic |
| ACACCGGATCTATTTGGTGG+CGG | 0.626807 | 3_1:+75230243 | Msa0375460:CDS |
| TGGGGAGTAGGAGTATTACT+GGG | 0.634686 | 3_1:+75231222 | Msa0375460:CDS |
| AGTGAGGCAGACATGAGACA+TGG | 0.648452 | 3_1:-75230172 | None:intergenic |
| TGAGGCAGACATGAGACATG+GGG | 0.699030 | 3_1:-75230170 | None:intergenic |
| TTAGAAGATGTGCACCAGTG+AGG | 0.702752 | 3_1:-75230188 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTAACATTTAAAAAAAGTC+AGG | - | chr3_1:75230866-75230885 | None:intergenic | 15.0% |
| !! | ATTGAAAATATTATACTACA+GGG | - | chr3_1:75231047-75231066 | None:intergenic | 15.0% |
| !! | ATTAACATTTAAAAAAAGTC+AGG | - | chr3_1:75230866-75230885 | None:intergenic | 15.0% |
| !! | ATTGAAAATATTATACTACA+GGG | - | chr3_1:75231047-75231066 | None:intergenic | 15.0% |
| !!! | AAAAACATGTAAAAGCATAA+TGG | - | chr3_1:75230341-75230360 | None:intergenic | 20.0% |
| !! | ATAACTTAATATGCATTGAA+TGG | + | chr3_1:75230720-75230739 | Msa0375460:intron | 20.0% |
| !! | ATAACTTAATATGCATTGAA+TGG | + | chr3_1:75230720-75230739 | Msa0375460:intron | 20.0% |
| !! | GATTGAAAATATTATACTAC+AGG | - | chr3_1:75231048-75231067 | None:intergenic | 20.0% |
| !!! | AGTTTTAGTTTTTATGTATG+TGG | + | chr3_1:75231073-75231092 | Msa0375460:intron | 20.0% |
| !!! | AAAAACATGTAAAAGCATAA+TGG | - | chr3_1:75230341-75230360 | None:intergenic | 20.0% |
| !! | ATAACTTAATATGCATTGAA+TGG | + | chr3_1:75230720-75230739 | Msa0375460:intron | 20.0% |
| !! | ATAACTTAATATGCATTGAA+TGG | + | chr3_1:75230720-75230739 | Msa0375460:intron | 20.0% |
| !! | GATTGAAAATATTATACTAC+AGG | - | chr3_1:75231048-75231067 | None:intergenic | 20.0% |
| !!! | AGTTTTAGTTTTTATGTATG+TGG | + | chr3_1:75231073-75231092 | Msa0375460:intron | 20.0% |
| ! | TTATTAACATTGAAACAGTC+GGG | - | chr3_1:75230704-75230723 | None:intergenic | 25.0% |
| ! | GTTATTAACATTGAAACAGT+CGG | - | chr3_1:75230705-75230724 | None:intergenic | 25.0% |
| ! | AAGAAAACAAAATGTGTAGA+TGG | - | chr3_1:75231192-75231211 | None:intergenic | 25.0% |
| !!! | CTACACATTTTGTTTTCTTT+AGG | + | chr3_1:75231193-75231212 | Msa0375460:intron | 25.0% |
| !! | TTGTTTTCTTTAGGTAAGTA+TGG | + | chr3_1:75231202-75231221 | Msa0375460:intron | 25.0% |
| !! | TGTTTTCTTTAGGTAAGTAT+GGG | + | chr3_1:75231203-75231222 | Msa0375460:intron | 25.0% |
| ! | TTATTAACATTGAAACAGTC+GGG | - | chr3_1:75230704-75230723 | None:intergenic | 25.0% |
| ! | GTTATTAACATTGAAACAGT+CGG | - | chr3_1:75230705-75230724 | None:intergenic | 25.0% |
| ! | AAGAAAACAAAATGTGTAGA+TGG | - | chr3_1:75231192-75231211 | None:intergenic | 25.0% |
| !!! | CTACACATTTTGTTTTCTTT+AGG | + | chr3_1:75231193-75231212 | Msa0375460:intron | 25.0% |
| !! | TTGTTTTCTTTAGGTAAGTA+TGG | + | chr3_1:75231202-75231221 | Msa0375460:intron | 25.0% |
| !! | TGTTTTCTTTAGGTAAGTAT+GGG | + | chr3_1:75231203-75231222 | Msa0375460:intron | 25.0% |
| ATTGATGAACTTCACTAACA+AGG | - | chr3_1:75230642-75230661 | None:intergenic | 30.0% | |
| CTTAATATGCATTGAATGGT+AGG | + | chr3_1:75230724-75230743 | Msa0375460:intron | 30.0% | |
| CAAGCTAAAAGAACTTTCTA+TGG | + | chr3_1:75230774-75230793 | Msa0375460:intron | 30.0% | |
| CAACTGTCTACTAACAAATT+TGG | + | chr3_1:75230807-75230826 | Msa0375460:intron | 30.0% | |
| CTTAATATGCATTGAATGGT+TGG | + | chr3_1:75230724-75230743 | Msa0375460:intron | 30.0% | |
| ATGCAACAATTGTCTACTAA+TGG | + | chr3_1:75230967-75230986 | Msa0375460:intron | 30.0% | |
| ! | GTTTTCTTTAGGTAAGTATG+GGG | + | chr3_1:75231204-75231223 | Msa0375460:intron | 30.0% |
| ATTGATGAACTTCACTAACA+AGG | - | chr3_1:75230642-75230661 | None:intergenic | 30.0% | |
| CTTAATATGCATTGAATGGT+AGG | + | chr3_1:75230724-75230743 | Msa0375460:intron | 30.0% | |
| CAAGCTAAAAGAACTTTCTA+TGG | + | chr3_1:75230774-75230793 | Msa0375460:intron | 30.0% | |
| CAACTGTCTACTAACAAATT+TGG | + | chr3_1:75230807-75230826 | Msa0375460:intron | 30.0% | |
| CTTAATATGCATTGAATGGT+TGG | + | chr3_1:75230724-75230743 | Msa0375460:intron | 30.0% | |
| ATGCAACAATTGTCTACTAA+TGG | + | chr3_1:75230967-75230986 | Msa0375460:intron | 30.0% | |
| ! | GTTTTCTTTAGGTAAGTATG+GGG | + | chr3_1:75231204-75231223 | Msa0375460:intron | 30.0% |
| GGAAGATGATCTAAGTCTAA+GGG | - | chr3_1:75230152-75230171 | None:intergenic | 35.0% | |
| ! | ATCTTCTAAGAGCTGCAATA+TGG | + | chr3_1:75230202-75230221 | Msa0375460:CDS | 35.0% |
| AGAAGTAAAGAACAGATCTG+AGG | - | chr3_1:75230312-75230331 | None:intergenic | 35.0% | |
| GAAAATGATGAACAGAACGA+AGG | - | chr3_1:75230368-75230387 | None:intergenic | 35.0% | |
| !! | TAGTTTTGCTGTGTTTTTCC+TGG | + | chr3_1:75230447-75230466 | Msa0375460:intron | 35.0% |
| ATTTGAGCTCAAACATTTCC+AGG | - | chr3_1:75230468-75230487 | None:intergenic | 35.0% | |
| TAGTAGTAGTAGTAGTAGTC+TGG | + | chr3_1:75230603-75230622 | Msa0375460:intron | 35.0% | |
| CTGTCTACTAACAAATTTGG+TGG | + | chr3_1:75230810-75230829 | Msa0375460:intron | 35.0% | |
| !!! | TATTTTAGCTTGAATCGAGC+AGG | - | chr3_1:75230930-75230949 | None:intergenic | 35.0% |
| ! | TGTGCAATTCTTATTTAGCC+TGG | + | chr3_1:75231113-75231132 | Msa0375460:intron | 35.0% |
| ATGGCATGTTCTTAGATCTT+GGG | - | chr3_1:75231173-75231192 | None:intergenic | 35.0% | |
| GGAAGATGATCTAAGTCTAA+GGG | - | chr3_1:75230152-75230171 | None:intergenic | 35.0% | |
| ! | ATCTTCTAAGAGCTGCAATA+TGG | + | chr3_1:75230202-75230221 | Msa0375460:CDS | 35.0% |
| AGAAGTAAAGAACAGATCTG+AGG | - | chr3_1:75230312-75230331 | None:intergenic | 35.0% | |
| GAAAATGATGAACAGAACGA+AGG | - | chr3_1:75230368-75230387 | None:intergenic | 35.0% | |
| !! | TAGTTTTGCTGTGTTTTTCC+TGG | + | chr3_1:75230447-75230466 | Msa0375460:intron | 35.0% |
| ATTTGAGCTCAAACATTTCC+AGG | - | chr3_1:75230468-75230487 | None:intergenic | 35.0% | |
| TAGTAGTAGTAGTAGTAGTC+TGG | + | chr3_1:75230603-75230622 | Msa0375460:intron | 35.0% | |
| CTGTCTACTAACAAATTTGG+TGG | + | chr3_1:75230810-75230829 | Msa0375460:intron | 35.0% | |
| !!! | TATTTTAGCTTGAATCGAGC+AGG | - | chr3_1:75230930-75230949 | None:intergenic | 35.0% |
| ! | TGTGCAATTCTTATTTAGCC+TGG | + | chr3_1:75231113-75231132 | Msa0375460:intron | 35.0% |
| ATGGCATGTTCTTAGATCTT+GGG | - | chr3_1:75231173-75231192 | None:intergenic | 35.0% | |
| ! | TCTAAGTCTAAGGGTGTGTT+TGG | - | chr3_1:75230143-75230162 | None:intergenic | 40.0% |
| GGGAAGATGATCTAAGTCTA+AGG | - | chr3_1:75230153-75230172 | None:intergenic | 40.0% | |
| AAATAGATCCGGTGTCTTAG+AGG | - | chr3_1:75230238-75230257 | None:intergenic | 40.0% | |
| ! | TCTAAGACACCGGATCTATT+TGG | + | chr3_1:75230237-75230256 | Msa0375460:CDS | 40.0% |
| ! | TCCTTTCAAGTGCTACTACA+AGG | + | chr3_1:75230281-75230300 | Msa0375460:CDS | 40.0% |
| ACCTTGTAGTAGCACTTGAA+AGG | - | chr3_1:75230285-75230304 | None:intergenic | 40.0% | |
| CTTCACTAACAAGGATTGCA+GGG | - | chr3_1:75230633-75230652 | None:intergenic | 40.0% | |
| ACTTCACTAACAAGGATTGC+AGG | - | chr3_1:75230634-75230653 | None:intergenic | 40.0% | |
| GGAGAAGAATGGAAGCTAAA+TGG | - | chr3_1:75230683-75230702 | None:intergenic | 40.0% | |
| TATGCATTGAATGGTTGGAG+TGG | + | chr3_1:75230893-75230912 | Msa0375460:intron | 40.0% | |
| TATTATACTACAGGGTGTGC+TGG | - | chr3_1:75231039-75231058 | None:intergenic | 40.0% | |
| ACCTAACAGACAATATGACC+AGG | - | chr3_1:75231134-75231153 | None:intergenic | 40.0% | |
| GATGGCATGTTCTTAGATCT+TGG | - | chr3_1:75231174-75231193 | None:intergenic | 40.0% | |
| TTTAGGTAAGTATGGGGAGT+AGG | + | chr3_1:75231210-75231229 | Msa0375460:intron | 40.0% | |
| ! | TCTAAGTCTAAGGGTGTGTT+TGG | - | chr3_1:75230143-75230162 | None:intergenic | 40.0% |
| GGGAAGATGATCTAAGTCTA+AGG | - | chr3_1:75230153-75230172 | None:intergenic | 40.0% | |
| AAATAGATCCGGTGTCTTAG+AGG | - | chr3_1:75230238-75230257 | None:intergenic | 40.0% | |
| ! | TCTAAGACACCGGATCTATT+TGG | + | chr3_1:75230237-75230256 | Msa0375460:CDS | 40.0% |
| ! | TCCTTTCAAGTGCTACTACA+AGG | + | chr3_1:75230281-75230300 | Msa0375460:CDS | 40.0% |
| ACCTTGTAGTAGCACTTGAA+AGG | - | chr3_1:75230285-75230304 | None:intergenic | 40.0% | |
| CTTCACTAACAAGGATTGCA+GGG | - | chr3_1:75230633-75230652 | None:intergenic | 40.0% | |
| ACTTCACTAACAAGGATTGC+AGG | - | chr3_1:75230634-75230653 | None:intergenic | 40.0% | |
| GGAGAAGAATGGAAGCTAAA+TGG | - | chr3_1:75230683-75230702 | None:intergenic | 40.0% | |
| TATGCATTGAATGGTTGGAG+TGG | + | chr3_1:75230893-75230912 | Msa0375460:intron | 40.0% | |
| TATTATACTACAGGGTGTGC+TGG | - | chr3_1:75231039-75231058 | None:intergenic | 40.0% | |
| ACCTAACAGACAATATGACC+AGG | - | chr3_1:75231134-75231153 | None:intergenic | 40.0% | |
| GATGGCATGTTCTTAGATCT+TGG | - | chr3_1:75231174-75231193 | None:intergenic | 40.0% | |
| TTTAGGTAAGTATGGGGAGT+AGG | + | chr3_1:75231210-75231229 | Msa0375460:intron | 40.0% | |
| ! | GTCTAAGGGTGTGTTTGGTT+TGG | - | chr3_1:75230138-75230157 | None:intergenic | 45.0% |
| TTAGAAGATGTGCACCAGTG+AGG | - | chr3_1:75230191-75230210 | None:intergenic | 45.0% | |
| AAGACACCGGATCTATTTGG+TGG | + | chr3_1:75230240-75230259 | Msa0375460:CDS | 45.0% | |
| TGAAACAGTCGGGAGAAGAA+TGG | - | chr3_1:75230694-75230713 | None:intergenic | 45.0% | |
| GCCTGGTCATATTGTCTGTT+AGG | + | chr3_1:75231130-75231149 | Msa0375460:intron | 45.0% | |
| !! | ATGGGGAGTAGGAGTATTAC+TGG | + | chr3_1:75231221-75231240 | Msa0375460:CDS | 45.0% |
| !! | TGGGGAGTAGGAGTATTACT+GGG | + | chr3_1:75231222-75231241 | Msa0375460:CDS | 45.0% |
| ! | GTCTAAGGGTGTGTTTGGTT+TGG | - | chr3_1:75230138-75230157 | None:intergenic | 45.0% |
| TTAGAAGATGTGCACCAGTG+AGG | - | chr3_1:75230191-75230210 | None:intergenic | 45.0% | |
| AAGACACCGGATCTATTTGG+TGG | + | chr3_1:75230240-75230259 | Msa0375460:CDS | 45.0% | |
| TGAAACAGTCGGGAGAAGAA+TGG | - | chr3_1:75230694-75230713 | None:intergenic | 45.0% | |
| GCCTGGTCATATTGTCTGTT+AGG | + | chr3_1:75231130-75231149 | Msa0375460:intron | 45.0% | |
| !! | ATGGGGAGTAGGAGTATTAC+TGG | + | chr3_1:75231221-75231240 | Msa0375460:CDS | 45.0% |
| !! | TGGGGAGTAGGAGTATTACT+GGG | + | chr3_1:75231222-75231241 | Msa0375460:CDS | 45.0% |
| TGAGGCAGACATGAGACATG+GGG | - | chr3_1:75230173-75230192 | None:intergenic | 50.0% | |
| GTGAGGCAGACATGAGACAT+GGG | - | chr3_1:75230174-75230193 | None:intergenic | 50.0% | |
| AGTGAGGCAGACATGAGACA+TGG | - | chr3_1:75230175-75230194 | None:intergenic | 50.0% | |
| ATGTCTCATGTCTGCCTCAC+TGG | + | chr3_1:75230174-75230193 | Msa0375460:CDS | 50.0% | |
| !! | ACGAGCTTCCTCTAAGACAC+CGG | + | chr3_1:75230227-75230246 | Msa0375460:CDS | 50.0% |
| ! | ACACCGGATCTATTTGGTGG+CGG | + | chr3_1:75230243-75230262 | Msa0375460:CDS | 50.0% |
| TCACCGCCACCAAATAGATC+CGG | - | chr3_1:75230249-75230268 | None:intergenic | 50.0% | |
| ATACTACAGGGTGTGCTGGA+GGG | - | chr3_1:75231035-75231054 | None:intergenic | 50.0% | |
| TATACTACAGGGTGTGCTGG+AGG | - | chr3_1:75231036-75231055 | None:intergenic | 50.0% | |
| TGAGGCAGACATGAGACATG+GGG | - | chr3_1:75230173-75230192 | None:intergenic | 50.0% | |
| GTGAGGCAGACATGAGACAT+GGG | - | chr3_1:75230174-75230193 | None:intergenic | 50.0% | |
| AGTGAGGCAGACATGAGACA+TGG | - | chr3_1:75230175-75230194 | None:intergenic | 50.0% | |
| ATGTCTCATGTCTGCCTCAC+TGG | + | chr3_1:75230174-75230193 | Msa0375460:CDS | 50.0% | |
| !! | ACGAGCTTCCTCTAAGACAC+CGG | + | chr3_1:75230227-75230246 | Msa0375460:CDS | 50.0% |
| ! | ACACCGGATCTATTTGGTGG+CGG | + | chr3_1:75230243-75230262 | Msa0375460:CDS | 50.0% |
| TCACCGCCACCAAATAGATC+CGG | - | chr3_1:75230249-75230268 | None:intergenic | 50.0% | |
| ATACTACAGGGTGTGCTGGA+GGG | - | chr3_1:75231035-75231054 | None:intergenic | 50.0% | |
| TATACTACAGGGTGTGCTGG+AGG | - | chr3_1:75231036-75231055 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_1 | gene | 75230111 | 75231256 | 75230111 | ID=Msa0375460;Name=Msa0375460 |
| chr3_1 | mRNA | 75230111 | 75231256 | 75230111 | ID=Msa0375460-mRNA-1;Parent=Msa0375460;Name=Msa0375460-mRNA-1;_AED=0.52;_eAED=0.52;_QI=63|0|0.5|0.5|1|1|2|0|56 |
| chr3_1 | exon | 75230111 | 75230302 | 75230111 | ID=Msa0375460-mRNA-1:exon:17599;Parent=Msa0375460-mRNA-1 |
| chr3_1 | exon | 75231215 | 75231256 | 75231215 | ID=Msa0375460-mRNA-1:exon:17600;Parent=Msa0375460-mRNA-1 |
| chr3_1 | five_prime_UTR | 75230111 | 75230173 | 75230111 | ID=Msa0375460-mRNA-1:five_prime_utr;Parent=Msa0375460-mRNA-1 |
| chr3_1 | CDS | 75230174 | 75230302 | 75230174 | ID=Msa0375460-mRNA-1:cds;Parent=Msa0375460-mRNA-1 |
| chr3_1 | CDS | 75231215 | 75231256 | 75231215 | ID=Msa0375460-mRNA-1:cds;Parent=Msa0375460-mRNA-1 |
| Gene Sequence |
| Protein sequence |
