Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0380190 | AES97217.1 | 52.874 | 87 | 18 | 2 | 5 | 91 | 3 | 66 | 2.09e-17 | 84.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0380190 | G7JY58 | 52.874 | 87 | 18 | 2 | 5 | 91 | 3 | 66 | 9.97e-18 | 84.3 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0017510 | Msa0380190 | 0.808534 | 2.954838e-50 | -8.615850e-47 |
| Msa0142220 | Msa0380190 | 0.802793 | 4.739427e-49 | -8.615850e-47 |
| Msa0157290 | Msa0380190 | 0.808109 | 3.639101e-50 | -8.615850e-47 |
| Msa0188830 | Msa0380190 | 0.812697 | 3.722126e-51 | -8.615850e-47 |
| Msa0380190 | Msa0380200 | 0.807581 | 4.713578e-50 | -8.615850e-47 |
| Msa0380190 | Msa0657970 | 0.804754 | 1.856087e-49 | -8.615850e-47 |
| Msa0380190 | Msa0811080 | 0.810793 | 9.662008e-51 | -8.615850e-47 |
| Msa0380190 | Msa0848060 | 0.816089 | 6.618772e-52 | -8.615850e-47 |
| Msa0380190 | Msa0880140 | 0.803239 | 3.833847e-49 | -8.615850e-47 |
| Msa0380190 | Msa0885280 | 0.808314 | 3.291578e-50 | -8.615850e-47 |
| Msa0380190 | Msa1000050 | 0.803842 | 2.873452e-49 | -8.615850e-47 |
| Msa0380190 | Msa1024450 | 0.992242 | 3.947865e-192 | -8.615850e-47 |
| Msa0380190 | Msa1136090 | 0.809544 | 1.795203e-50 | -8.615850e-47 |
| Msa0380190 | Msa1160670 | 0.819258 | 1.276293e-52 | -8.615850e-47 |
| Msa0380190 | Msa1171910 | 0.800300 | 1.537771e-48 | -8.615850e-47 |
| Msa0380190 | Msa1392260 | 0.811425 | 7.046138e-51 | -8.615850e-47 |
| Msa0380190 | Msa1429900 | 0.802730 | 4.884281e-49 | -8.615850e-47 |
| Msa0300570 | Msa0380190 | 0.800840 | 1.193060e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 8 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTTTGCTACTGCTATAAA+TGG | 0.198718 | 3_1:+81854194 | Msa0380190:CDS |
| ATAATGAATGTGAGTGTTAA+AGG | 0.210597 | 3_1:+81853805 | None:intergenic |
| ATAGCAGTAATAACAACTTA+TGG | 0.399494 | 3_1:-81854165 | None:intergenic |
| GCTCCTTTATGATTTGATAC+AGG | 0.399634 | 3_1:-81854143 | None:intergenic |
| ATATTGATATACATCAACTT+TGG | 0.459206 | 3_1:+81853899 | Msa0380190:CDS |
| AAACCTGTATCAAATCATAA+AGG | 0.464881 | 3_1:+81854140 | Msa0380190:CDS |
| GCTTTGCTACTGCTATAAAT+GGG | 0.548900 | 3_1:+81854195 | Msa0380190:CDS |
| TATACATCAACTTTGGCTTG+AGG | 0.606702 | 3_1:+81853906 | Msa0380190:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAATCATAATATAGAATA+GGG | - | chr3_1:81854105-81854124 | None:intergenic | 10.0% |
| !! | AAAAAATCATAATATAGAAT+AGG | - | chr3_1:81854106-81854125 | None:intergenic | 10.0% |
| !! | ATATTGATATACATCAACTT+TGG | + | chr3_1:81853899-81853918 | Msa0380190:CDS | 20.0% |
| !!! | TGATTTTTTCTAAATTTGCT+AGG | + | chr3_1:81854117-81854136 | Msa0380190:CDS | 20.0% |
| ! | TTAATGCTAAAATAGCATCA+AGG | - | chr3_1:81853983-81854002 | None:intergenic | 25.0% |
| ! | AAACCTGTATCAAATCATAA+AGG | + | chr3_1:81854140-81854159 | Msa0380190:CDS | 25.0% |
| ! | ATAGCAGTAATAACAACTTA+TGG | - | chr3_1:81854168-81854187 | None:intergenic | 25.0% |
| !!! | AAGTTTTTTCAATCAGATTC+TGG | + | chr3_1:81854230-81854249 | Msa0380190:CDS | 25.0% |
| !!! | AGTTTTTTCAATCAGATTCT+GGG | + | chr3_1:81854231-81854250 | Msa0380190:CDS | 25.0% |
| !! | TATACATCAACTTTGGCTTG+AGG | + | chr3_1:81853906-81853925 | Msa0380190:CDS | 35.0% |
| !! | CTTGAGGCAGATTTTTGTTT+AGG | + | chr3_1:81853922-81853941 | Msa0380190:CDS | 35.0% |
| GCTCCTTTATGATTTGATAC+AGG | - | chr3_1:81854146-81854165 | None:intergenic | 35.0% | |
| TGCTTTGCTACTGCTATAAA+TGG | + | chr3_1:81854194-81854213 | Msa0380190:CDS | 35.0% | |
| GCTTTGCTACTGCTATAAAT+GGG | + | chr3_1:81854195-81854214 | Msa0380190:CDS | 35.0% | |
| !! | ATTCTGGGTCATTTTCTTCA+TGG | + | chr3_1:81854246-81854265 | Msa0380190:CDS | 35.0% |
| !! | GAGGCAGATTTTTGTTTAGG+TGG | + | chr3_1:81853925-81853944 | Msa0380190:CDS | 40.0% |
| CATTAATCAACCTGCACGAG+TGG | + | chr3_1:81853997-81854016 | Msa0380190:intron | 45.0% | |
| GCAAAAGCAGCCACTCGTGC+AGG | - | chr3_1:81854010-81854029 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_1 | gene | 81853808 | 81854271 | 81853808 | ID=Msa0380190;Name=Msa0380190 |
| chr3_1 | mRNA | 81853808 | 81854271 | 81853808 | ID=Msa0380190-mRNA-1;Parent=Msa0380190;Name=Msa0380190-mRNA-1;_AED=0.46;_eAED=0.50;_QI=0|0|0|1|0|0|2|0|112 |
| chr3_1 | exon | 81853808 | 81853946 | 81853808 | ID=Msa0380190-mRNA-1:exon:20825;Parent=Msa0380190-mRNA-1 |
| chr3_1 | exon | 81854072 | 81854271 | 81854072 | ID=Msa0380190-mRNA-1:exon:20826;Parent=Msa0380190-mRNA-1 |
| chr3_1 | CDS | 81853808 | 81853946 | 81853808 | ID=Msa0380190-mRNA-1:cds;Parent=Msa0380190-mRNA-1 |
| chr3_1 | CDS | 81854072 | 81854271 | 81854072 | ID=Msa0380190-mRNA-1:cds;Parent=Msa0380190-mRNA-1 |
| Gene Sequence |
| Protein sequence |
