Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1024450 | AES97217.1 | 52.874 | 87 | 18 | 2 | 5 | 91 | 3 | 66 | 2.09e-17 | 84.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1024450 | G7JY58 | 52.874 | 87 | 18 | 2 | 5 | 91 | 3 | 66 | 9.97e-18 | 84.3 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0017510 | Msa1024450 | 0.804938 | 1.698393e-49 | -8.615850e-47 |
| Msa0657970 | Msa1024450 | 0.809024 | 2.320951e-50 | -8.615850e-47 |
| Msa0766630 | Msa1024450 | 0.812515 | 4.078713e-51 | -8.615850e-47 |
| Msa0811080 | Msa1024450 | 0.807975 | 3.887531e-50 | -8.615850e-47 |
| Msa0142220 | Msa1024450 | 0.808950 | 2.407369e-50 | -8.615850e-47 |
| Msa0153910 | Msa1024450 | 0.800237 | 1.583948e-48 | -8.615850e-47 |
| Msa0157290 | Msa1024450 | 0.804549 | 2.047613e-49 | -8.615850e-47 |
| Msa0188830 | Msa1024450 | 0.818305 | 2.100558e-52 | -8.615850e-47 |
| Msa0231000 | Msa1024450 | 0.801833 | 7.471179e-49 | -8.615850e-47 |
| Msa0380190 | Msa1024450 | 0.992242 | 3.947865e-192 | -8.615850e-47 |
| Msa0380200 | Msa1024450 | 0.806981 | 6.317802e-50 | -8.615850e-47 |
| Msa0432300 | Msa1024450 | 0.803327 | 3.676306e-49 | -8.615850e-47 |
| Msa1000050 | Msa1024450 | 0.807446 | 5.036085e-50 | -8.615850e-47 |
| Msa1014540 | Msa1024450 | 0.802265 | 6.090297e-49 | -8.615850e-47 |
| Msa1024450 | Msa1136090 | 0.807607 | 4.654747e-50 | -8.615850e-47 |
| Msa1024450 | Msa1160670 | 0.816955 | 4.233611e-52 | -8.615850e-47 |
| Msa1024450 | Msa1162550 | -0.801539 | 8.585402e-49 | -8.615850e-47 |
| Msa1024450 | Msa1171910 | 0.800473 | 1.417926e-48 | -8.615850e-47 |
| Msa1024450 | Msa1249490 | 0.806431 | 8.252790e-50 | -8.615850e-47 |
| Msa1024450 | Msa1392260 | 0.816857 | 4.454710e-52 | -8.615850e-47 |
| Msa1024450 | Msa1429900 | 0.809628 | 1.722537e-50 | -8.615850e-47 |
| Msa0309020 | Msa1024450 | 0.801550 | 8.539232e-49 | -8.615850e-47 |
| Msa0345970 | Msa1024450 | 0.801325 | 9.497583e-49 | -8.615850e-47 |
| Msa0848060 | Msa1024450 | 0.815495 | 8.979650e-52 | -8.615850e-47 |
| Msa0885280 | Msa1024450 | 0.807186 | 5.715594e-50 | -8.615850e-47 |
| Msa0907260 | Msa1024450 | 0.801957 | 7.045292e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 8 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTTTGCTACTGCTATAAA+TGG | 0.198718 | 7_1:-53782848 | Msa1024450:CDS |
| ATAATGAATGTGAGTGTTAA+AGG | 0.210597 | 7_1:-53783237 | Msa1024450:CDS |
| ATAGCAGTAATAACAACTTA+TGG | 0.399494 | 7_1:+53782877 | None:intergenic |
| GCTCCTTTATGATTTGATAC+AGG | 0.399634 | 7_1:+53782899 | None:intergenic |
| ATATTGATATACATCAACTT+TGG | 0.459206 | 7_1:-53783143 | Msa1024450:CDS |
| AAACCTGTATCAAATCATAA+AGG | 0.464881 | 7_1:-53782902 | Msa1024450:CDS |
| GCTTTGCTACTGCTATAAAT+GGG | 0.548900 | 7_1:-53782847 | Msa1024450:CDS |
| TATACATCAACTTTGGCTTG+AGG | 0.606702 | 7_1:-53783136 | Msa1024450:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAATCATAATATAGAATA+GGG | + | chr7_1:53783090-53783109 | None:intergenic | 10.0% |
| !! | AAAAAATCATAATATAGAAT+AGG | + | chr7_1:53783091-53783110 | None:intergenic | 10.0% |
| !! | ATATTGATATACATCAACTT+TGG | - | chr7_1:53782884-53782903 | Msa1024450:CDS | 20.0% |
| !!! | TGATTTTTTCTAAATTTGCT+AGG | - | chr7_1:53783102-53783121 | Msa1024450:intron | 20.0% |
| ! | TTAATGCTAAAATAGCATCA+AGG | + | chr7_1:53782968-53782987 | None:intergenic | 25.0% |
| ! | AAACCTGTATCAAATCATAA+AGG | - | chr7_1:53783125-53783144 | Msa1024450:CDS | 25.0% |
| ! | ATAGCAGTAATAACAACTTA+TGG | + | chr7_1:53783153-53783172 | None:intergenic | 25.0% |
| !!! | AAGTTTTTTCAATCAGATTC+TGG | - | chr7_1:53783215-53783234 | Msa1024450:CDS | 25.0% |
| !!! | AGTTTTTTCAATCAGATTCT+GGG | - | chr7_1:53783216-53783235 | Msa1024450:CDS | 25.0% |
| !! | TATACATCAACTTTGGCTTG+AGG | - | chr7_1:53782891-53782910 | Msa1024450:CDS | 35.0% |
| !! | CTTGAGGCAGATTTTTGTTT+AGG | - | chr7_1:53782907-53782926 | Msa1024450:CDS | 35.0% |
| GCTCCTTTATGATTTGATAC+AGG | + | chr7_1:53783131-53783150 | None:intergenic | 35.0% | |
| TGCTTTGCTACTGCTATAAA+TGG | - | chr7_1:53783179-53783198 | Msa1024450:CDS | 35.0% | |
| GCTTTGCTACTGCTATAAAT+GGG | - | chr7_1:53783180-53783199 | Msa1024450:CDS | 35.0% | |
| !! | ATTCTGGGTCATTTTCTTCA+TGG | - | chr7_1:53783231-53783250 | Msa1024450:CDS | 35.0% |
| !! | GAGGCAGATTTTTGTTTAGG+TGG | - | chr7_1:53782910-53782929 | Msa1024450:CDS | 40.0% |
| CATTAATCAACCTGCACGAG+TGG | - | chr7_1:53782982-53783001 | Msa1024450:intron | 45.0% | |
| GCAAAAGCAGCCACTCGTGC+AGG | + | chr7_1:53782995-53783014 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_1 | gene | 53782793 | 53783256 | 53782793 | ID=Msa1024450;Name=Msa1024450 |
| chr7_1 | mRNA | 53782793 | 53783256 | 53782793 | ID=Msa1024450-mRNA-1;Parent=Msa1024450;Name=Msa1024450-mRNA-1;_AED=0.46;_eAED=0.49;_QI=0|0|0|1|0|0|2|0|112 |
| chr7_1 | exon | 53783118 | 53783256 | 53783118 | ID=Msa1024450-mRNA-1:exon:10641;Parent=Msa1024450-mRNA-1 |
| chr7_1 | exon | 53782793 | 53782992 | 53782793 | ID=Msa1024450-mRNA-1:exon:10640;Parent=Msa1024450-mRNA-1 |
| chr7_1 | CDS | 53783118 | 53783256 | 53783118 | ID=Msa1024450-mRNA-1:cds;Parent=Msa1024450-mRNA-1 |
| chr7_1 | CDS | 53782793 | 53782992 | 53782793 | ID=Msa1024450-mRNA-1:cds;Parent=Msa1024450-mRNA-1 |
| Gene Sequence |
| Protein sequence |
