AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0380410

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0380410 MtrunA17_Chr3g0145951 99.119 227 2 0 1 227 1 227 1.98e-158 437
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0380410 AT1G16210.1 73.009 226 58 1 1 223 1 226 1.16e-106 307

Find 97 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCGAGTGTATTTAAAATT+TGG 0.164169 3_1:-82063597 None:intergenic
CGCCTCTTCCTCCCTCTTCT+TGG 0.214378 3_1:-82062626 None:intergenic
AAGACAAGTTCAAAGATTAT+TGG 0.240090 3_1:-82063546 None:intergenic
CGTTTCCGCGGCGCTCTTGC+GGG 0.269884 3_1:-82062527 None:intergenic
CGCCGCGGAAACGGAGAAAA+AGG 0.310222 3_1:+82062537 Msa0380410:CDS
AGGACATGATTTGGAAGCTT+TGG 0.331210 3_1:+82063176 Msa0380410:CDS
GCATCTTTAATTCACCTAGT+AGG 0.334052 3_1:-82062462 None:intergenic
ACCCTGTTTGCCTTCTTGTC+AGG 0.352573 3_1:-82062739 None:intergenic
GGTTTCTCTTGCTTCAACTT+AGG 0.359376 3_1:-82063130 None:intergenic
CACAGTACAAGGACATGATT+TGG 0.361884 3_1:+82063167 Msa0380410:CDS
CGCCTCTCTGGATGCCTGTC+GGG 0.367827 3_1:-82062988 None:intergenic
CAAAGATTATTGGTTGTAGT+AGG 0.367989 3_1:-82063536 None:intergenic
CGCCAAGAAGAGGGAGGAAG+AGG 0.386559 3_1:+82062624 Msa0380410:CDS
CCGCAAGAGCGCCGCGGAAA+CGG 0.388342 3_1:+82062528 Msa0380410:CDS
GAAGCCTTGAGGCGCCTCTC+TGG 0.388885 3_1:-82063000 None:intergenic
GAGTGAGTGACTTGATTAAG+AGG 0.390157 3_1:-82063217 None:intergenic
CCTAGTAGGGTTTCGCTCGC+TGG 0.400470 3_1:-82062448 None:intergenic
GCCTCCGTCACCTTCGGTAC+AGG 0.404250 3_1:-82062769 None:intergenic
GGTGCGTCGATTGGCGGAGC+TGG 0.407431 3_1:+82062684 Msa0380410:CDS
CCGTTTCCGCGGCGCTCTTG+CGG 0.414275 3_1:-82062528 None:intergenic
CATCTTTAATTCACCTAGTA+GGG 0.414363 3_1:-82062461 None:intergenic
AGCCTGACAAGAAGGCAAAC+AGG 0.415814 3_1:+82062737 Msa0380410:CDS
AAAGATGGGTGTGAATTCTA+AGG 0.416000 3_1:+82062489 Msa0380410:CDS
GGAGGAAGAGGCGGATAAGA+AGG 0.418038 3_1:+82062636 Msa0380410:CDS
CTTGCGGGCCCTCGCCGCCT+CGG 0.418040 3_1:-82062512 None:intergenic
GGATAAGAAGGCGGAGGCGG+CGG 0.424210 3_1:+82062648 Msa0380410:CDS
GAGAGAAGGAGGAGCAGTAC+TGG 0.439791 3_1:+82062572 Msa0380410:CDS
ACTTGATTAAGAGGATTGTC+AGG 0.442090 3_1:-82063208 None:intergenic
GCAAGCTATGGCACAGAAGA+AGG 0.443447 3_1:+82062819 Msa0380410:CDS
GGCGGATAAGAAGGCGGAGG+CGG 0.454208 3_1:+82062645 Msa0380410:CDS
GAAGAGGACTGCTGGGGAGG+AGG 0.460637 3_1:+82062855 Msa0380410:CDS
TTCTAAGGCCGAGGCGGCGA+GGG 0.465736 3_1:+82062504 Msa0380410:CDS
GAAGCGAAGAAGAGGACTGC+TGG 0.465817 3_1:+82062847 Msa0380410:CDS
GCGCCTCAAGGCTTCCTTCA+AGG 0.472754 3_1:+82063008 Msa0380410:CDS
AGCGGAGGTGCGTCGATTGG+CGG 0.475781 3_1:+82062678 Msa0380410:CDS
AGCTCCGCCTCCGTCACCTT+CGG 0.476514 3_1:-82062775 None:intergenic
AAAACAGATACATACCTTGA+AGG 0.481347 3_1:-82063022 None:intergenic
GCGCCTCTCTGGATGCCTGT+CGG 0.482921 3_1:-82062989 None:intergenic
TACTGTGTGTATGTAAGCCC+TGG 0.485764 3_1:-82063151 None:intergenic
GGAGGCGGCGGCACGACGAG+CGG 0.491424 3_1:+82062660 Msa0380410:CDS
AAGCGAAGAAGAGGACTGCT+GGG 0.493135 3_1:+82062848 Msa0380410:CDS
GATGGCTTCGTCAAGAGTGC+TGG 0.493876 3_1:-82062935 None:intergenic
CGGAGGCGGAGCTGAGGAAG+CGG 0.494922 3_1:+82062785 Msa0380410:CDS
CCAGCGAGCGAAACCCTACT+AGG 0.505208 3_1:+82062448 Msa0380410:five_prime_UTR
GCTGAGGAAGCGGCGAGAGG+AGG 0.505549 3_1:+82062795 Msa0380410:CDS
TCCCTCTTCTTGGCGGCGCG+TGG 0.507668 3_1:-82062616 None:intergenic
CTCTTCCTCCCTCTTCTTGG+CGG 0.507806 3_1:-82062623 None:intergenic
AGATTATTGGTTGTAGTAGG+AGG 0.510390 3_1:-82063533 None:intergenic
GCCACGCGCCGCCAAGAAGA+GGG 0.513629 3_1:+82062615 Msa0380410:CDS
AGCCACGCGCCGCCAAGAAG+AGG 0.516989 3_1:+82062614 Msa0380410:CDS
GCGAGAGGAGGAGCAAGCTA+TGG 0.521774 3_1:+82062807 Msa0380410:CDS
ACGAGCGGAGGTGCGTCGAT+TGG 0.522062 3_1:+82062675 Msa0380410:CDS
GGCGGCGGCACGACGAGCGG+AGG 0.533711 3_1:+82062663 Msa0380410:CDS
ACTTAATCATGAATTTGAGA+TGG 0.534693 3_1:-82063384 None:intergenic
CCTCCGTCACCTTCGGTACA+GGG 0.541244 3_1:-82062768 None:intergenic
GGAGCTGAGGAAGCGGCGAG+AGG 0.552884 3_1:+82062792 Msa0380410:CDS
GCCTGACAAGAAGGCAAACA+GGG 0.555319 3_1:+82062738 Msa0380410:CDS
GCTTACATACACACAGTACA+AGG 0.555636 3_1:+82063156 Msa0380410:CDS
CAAGAAGAGGGAGGAAGAGG+CGG 0.555830 3_1:+82062627 Msa0380410:CDS
GGGTGTGAATTCTAAGGCCG+AGG 0.555835 3_1:+82062495 Msa0380410:CDS
GAAGAAGAGGACTGCTGGGG+AGG 0.559520 3_1:+82062852 Msa0380410:CDS
AGGTGACGGAGGCGGAGCTG+AGG 0.562961 3_1:+82062779 Msa0380410:CDS
AGAGGCGGATAAGAAGGCGG+AGG 0.563473 3_1:+82062642 Msa0380410:CDS
TGTACCGAAGGTGACGGAGG+CGG 0.565468 3_1:+82062771 Msa0380410:CDS
AGGCGGAGGAAGCGAAGAAG+AGG 0.566562 3_1:+82062839 Msa0380410:CDS
GATTCAATAAAGTCATGGCT+TGG 0.566810 3_1:+82063339 Msa0380410:three_prime_UTR
CTCTCTGGATGCCTGTCGGG+AGG 0.568049 3_1:-82062985 None:intergenic
GCGTCGATTGGCGGAGCTGG+AGG 0.569855 3_1:+82062687 Msa0380410:CDS
GCATCCAGAGAGGCGCCTCA+AGG 0.574178 3_1:+82062996 Msa0380410:CDS
ATCGATACTCATCTGAGCGA+TGG 0.576376 3_1:-82062953 None:intergenic
AGCAGCAACTGCTGAAGCAG+AGG 0.579802 3_1:+82062408 Msa0380410:five_prime_UTR
ATTCTAAGGCCGAGGCGGCG+AGG 0.588501 3_1:+82062503 Msa0380410:CDS
TCGGGAGACTCGAGAGAAGG+AGG 0.591271 3_1:+82062561 Msa0380410:CDS
TCAATAAAGTCATGGCTTGG+CGG 0.593024 3_1:+82063342 Msa0380410:three_prime_UTR
ATAACAACTTACCTCCCGAC+AGG 0.598312 3_1:+82062974 Msa0380410:CDS
ACGCGCCGCCAAGAAGAGGG+AGG 0.598349 3_1:+82062618 Msa0380410:CDS
GAGGGCCCGCAAGAGCGCCG+CGG 0.599591 3_1:+82062522 Msa0380410:CDS
GGAGCTGGAGGAGAAAGAGC+TGG 0.599937 3_1:+82062699 Msa0380410:CDS
AAGTTGAAGCAAGAGAAACC+AGG 0.604189 3_1:+82063133 Msa0380410:CDS
GGATCGGGAGACTCGAGAGA+AGG 0.606508 3_1:+82062558 Msa0380410:CDS
CCCTGTACCGAAGGTGACGG+AGG 0.608676 3_1:+82062768 Msa0380410:CDS
TATGGCACAGAAGAAGGCGG+AGG 0.610578 3_1:+82062825 Msa0380410:CDS
ATGATGATTCAATAAAGTCA+TGG 0.612785 3_1:+82063334 Msa0380410:three_prime_UTR
GGAGGAGGAGTACGAGAGAG+TGG 0.614209 3_1:+82062870 Msa0380410:CDS
GGTGTCTATCCCTGTACCGA+AGG 0.615935 3_1:+82062759 Msa0380410:CDS
TTATTGAATCATCATTCACT+CGG 0.617967 3_1:-82063326 None:intergenic
GGAAGAGGCGGATAAGAAGG+CGG 0.620199 3_1:+82062639 Msa0380410:CDS
TATCCCTGTACCGAAGGTGA+CGG 0.630098 3_1:+82062765 Msa0380410:CDS
CTCCCGACAGGCATCCAGAG+AGG 0.637354 3_1:+82062986 Msa0380410:CDS
GGAGGAGCAGTACTGGCGAG+AGG 0.649799 3_1:+82062579 Msa0380410:CDS
TCATATACTACTGCTTACAG+AGG 0.659085 3_1:-82063441 None:intergenic
TGTGAATTCTAAGGCCGAGG+CGG 0.660597 3_1:+82062498 Msa0380410:CDS
AGTTGAAGCAAGAGAAACCA+GGG 0.661223 3_1:+82063134 Msa0380410:CDS
CATATACTACTGCTTACAGA+GGG 0.665172 3_1:-82063440 None:intergenic
AGCGAAGAAGAGGACTGCTG+GGG 0.681467 3_1:+82062849 Msa0380410:CDS
ATACCTTGAAGGAAGCCTTG+AGG 0.684866 3_1:-82063011 None:intergenic
AGCTATGGCACAGAAGAAGG+CGG 0.695332 3_1:+82062822 Msa0380410:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATAACAAAAATAAAAAGAT+AGG - chr3_1:82063271-82063290 None:intergenic 15.0%
!! GATAACAAAAATAAAAAGAT+AGG - chr3_1:82063271-82063290 None:intergenic 15.0%
!!! TATCGAGTGTATTTAAAATT+TGG - chr3_1:82063600-82063619 None:intergenic 20.0%
!!! TATCGAGTGTATTTAAAATT+TGG - chr3_1:82063600-82063619 None:intergenic 20.0%
! ATAAAAAGATAGGCTAAAAG+TGG - chr3_1:82063261-82063280 None:intergenic 25.0%
! TTATTGAATCATCATTCACT+CGG - chr3_1:82063329-82063348 None:intergenic 25.0%
! ATGATGATTCAATAAAGTCA+TGG + chr3_1:82063334-82063353 Msa0380410:three_prime_UTR 25.0%
! ACTTAATCATGAATTTGAGA+TGG - chr3_1:82063387-82063406 None:intergenic 25.0%
!!! ACAAACATTGTTTGTTTTCA+TGG + chr3_1:82063493-82063512 Msa0380410:three_prime_UTR 25.0%
! AAGACAAGTTCAAAGATTAT+TGG - chr3_1:82063549-82063568 None:intergenic 25.0%
! ATAAAAAGATAGGCTAAAAG+TGG - chr3_1:82063261-82063280 None:intergenic 25.0%
! TTATTGAATCATCATTCACT+CGG - chr3_1:82063329-82063348 None:intergenic 25.0%
! ATGATGATTCAATAAAGTCA+TGG + chr3_1:82063334-82063353 Msa0380410:three_prime_UTR 25.0%
! ACTTAATCATGAATTTGAGA+TGG - chr3_1:82063387-82063406 None:intergenic 25.0%
!!! ACAAACATTGTTTGTTTTCA+TGG + chr3_1:82063493-82063512 Msa0380410:three_prime_UTR 25.0%
! AAGACAAGTTCAAAGATTAT+TGG - chr3_1:82063549-82063568 None:intergenic 25.0%
CATCTTTAATTCACCTAGTA+GGG - chr3_1:82062464-82062483 None:intergenic 30.0%
ATTAAAGATGCCCAAAAAGA+TGG + chr3_1:82062474-82062493 Msa0380410:exon 30.0%
TTAAAGATGCCCAAAAAGAT+GGG + chr3_1:82062475-82062494 Msa0380410:exon 30.0%
AAAACAGATACATACCTTGA+AGG - chr3_1:82063025-82063044 None:intergenic 30.0%
CATTCATTCATTCATTCATC+AGG + chr3_1:82063087-82063106 Msa0380410:intron 30.0%
!! CTAAAAGTGGATTATAAAGG+AGG - chr3_1:82063248-82063267 None:intergenic 30.0%
!! AGGCTAAAAGTGGATTATAA+AGG - chr3_1:82063251-82063270 None:intergenic 30.0%
! CAAAGATTATTGGTTGTAGT+AGG - chr3_1:82063539-82063558 None:intergenic 30.0%
CATCTTTAATTCACCTAGTA+GGG - chr3_1:82062464-82062483 None:intergenic 30.0%
ATTAAAGATGCCCAAAAAGA+TGG + chr3_1:82062474-82062493 Msa0380410:exon 30.0%
TTAAAGATGCCCAAAAAGAT+GGG + chr3_1:82062475-82062494 Msa0380410:exon 30.0%
AAAACAGATACATACCTTGA+AGG - chr3_1:82063025-82063044 None:intergenic 30.0%
CATTCATTCATTCATTCATC+AGG + chr3_1:82063087-82063106 Msa0380410:intron 30.0%
!! CTAAAAGTGGATTATAAAGG+AGG - chr3_1:82063248-82063267 None:intergenic 30.0%
!! AGGCTAAAAGTGGATTATAA+AGG - chr3_1:82063251-82063270 None:intergenic 30.0%
! CAAAGATTATTGGTTGTAGT+AGG - chr3_1:82063539-82063558 None:intergenic 30.0%
GCATCTTTAATTCACCTAGT+AGG - chr3_1:82062465-82062484 None:intergenic 35.0%
! GAATTCACACCCATCTTTTT+GGG - chr3_1:82062487-82062506 None:intergenic 35.0%
! AGAATTCACACCCATCTTTT+TGG - chr3_1:82062488-82062507 None:intergenic 35.0%
AAAGATGGGTGTGAATTCTA+AGG + chr3_1:82062489-82062508 Msa0380410:CDS 35.0%
GATTAAAAAGCCTGACAAGA+AGG + chr3_1:82062729-82062748 Msa0380410:CDS 35.0%
ACTTGATTAAGAGGATTGTC+AGG - chr3_1:82063211-82063230 None:intergenic 35.0%
GATTCAATAAAGTCATGGCT+TGG + chr3_1:82063339-82063358 Msa0380410:three_prime_UTR 35.0%
CATATACTACTGCTTACAGA+GGG - chr3_1:82063443-82063462 None:intergenic 35.0%
TCATATACTACTGCTTACAG+AGG - chr3_1:82063444-82063463 None:intergenic 35.0%
! AGATTATTGGTTGTAGTAGG+AGG - chr3_1:82063536-82063555 None:intergenic 35.0%
GCATCTTTAATTCACCTAGT+AGG - chr3_1:82062465-82062484 None:intergenic 35.0%
! GAATTCACACCCATCTTTTT+GGG - chr3_1:82062487-82062506 None:intergenic 35.0%
! AGAATTCACACCCATCTTTT+TGG - chr3_1:82062488-82062507 None:intergenic 35.0%
AAAGATGGGTGTGAATTCTA+AGG + chr3_1:82062489-82062508 Msa0380410:CDS 35.0%
GATTAAAAAGCCTGACAAGA+AGG + chr3_1:82062729-82062748 Msa0380410:CDS 35.0%
ACTTGATTAAGAGGATTGTC+AGG - chr3_1:82063211-82063230 None:intergenic 35.0%
GATTCAATAAAGTCATGGCT+TGG + chr3_1:82063339-82063358 Msa0380410:three_prime_UTR 35.0%
CATATACTACTGCTTACAGA+GGG - chr3_1:82063443-82063462 None:intergenic 35.0%
TCATATACTACTGCTTACAG+AGG - chr3_1:82063444-82063463 None:intergenic 35.0%
! AGATTATTGGTTGTAGTAGG+AGG - chr3_1:82063536-82063555 None:intergenic 35.0%
GGTTTCTCTTGCTTCAACTT+AGG - chr3_1:82063133-82063152 None:intergenic 40.0%
AAGTTGAAGCAAGAGAAACC+AGG + chr3_1:82063133-82063152 Msa0380410:CDS 40.0%
AGTTGAAGCAAGAGAAACCA+GGG + chr3_1:82063134-82063153 Msa0380410:CDS 40.0%
GCTTACATACACACAGTACA+AGG + chr3_1:82063156-82063175 Msa0380410:CDS 40.0%
CACAGTACAAGGACATGATT+TGG + chr3_1:82063167-82063186 Msa0380410:CDS 40.0%
! AGGACATGATTTGGAAGCTT+TGG + chr3_1:82063176-82063195 Msa0380410:CDS 40.0%
GAGTGAGTGACTTGATTAAG+AGG - chr3_1:82063220-82063239 None:intergenic 40.0%
! TCAATAAAGTCATGGCTTGG+CGG + chr3_1:82063342-82063361 Msa0380410:three_prime_UTR 40.0%
GGTTTCTCTTGCTTCAACTT+AGG - chr3_1:82063133-82063152 None:intergenic 40.0%
AAGTTGAAGCAAGAGAAACC+AGG + chr3_1:82063133-82063152 Msa0380410:CDS 40.0%
AGTTGAAGCAAGAGAAACCA+GGG + chr3_1:82063134-82063153 Msa0380410:CDS 40.0%
GCTTACATACACACAGTACA+AGG + chr3_1:82063156-82063175 Msa0380410:CDS 40.0%
CACAGTACAAGGACATGATT+TGG + chr3_1:82063167-82063186 Msa0380410:CDS 40.0%
! AGGACATGATTTGGAAGCTT+TGG + chr3_1:82063176-82063195 Msa0380410:CDS 40.0%
GAGTGAGTGACTTGATTAAG+AGG - chr3_1:82063220-82063239 None:intergenic 40.0%
! TCAATAAAGTCATGGCTTGG+CGG + chr3_1:82063342-82063361 Msa0380410:three_prime_UTR 40.0%
! ATCCTTTTTCTCCGTTTCCG+CGG - chr3_1:82062542-82062561 None:intergenic 45.0%
CGGAAACGGAGAAAAAGGAT+CGG + chr3_1:82062542-82062561 Msa0380410:CDS 45.0%
GGAAACGGAGAAAAAGGATC+GGG + chr3_1:82062543-82062562 Msa0380410:CDS 45.0%
ATCGATACTCATCTGAGCGA+TGG - chr3_1:82062956-82062975 None:intergenic 45.0%
ATAACAACTTACCTCCCGAC+AGG + chr3_1:82062974-82062993 Msa0380410:CDS 45.0%
ATACCTTGAAGGAAGCCTTG+AGG - chr3_1:82063014-82063033 None:intergenic 45.0%
TACTGTGTGTATGTAAGCCC+TGG - chr3_1:82063154-82063173 None:intergenic 45.0%
! ATCCTTTTTCTCCGTTTCCG+CGG - chr3_1:82062542-82062561 None:intergenic 45.0%
CGGAAACGGAGAAAAAGGAT+CGG + chr3_1:82062542-82062561 Msa0380410:CDS 45.0%
GGAAACGGAGAAAAAGGATC+GGG + chr3_1:82062543-82062562 Msa0380410:CDS 45.0%
ATCGATACTCATCTGAGCGA+TGG - chr3_1:82062956-82062975 None:intergenic 45.0%
ATAACAACTTACCTCCCGAC+AGG + chr3_1:82062974-82062993 Msa0380410:CDS 45.0%
ATACCTTGAAGGAAGCCTTG+AGG - chr3_1:82063014-82063033 None:intergenic 45.0%
TACTGTGTGTATGTAAGCCC+TGG - chr3_1:82063154-82063173 None:intergenic 45.0%
!! TGTGAATTCTAAGGCCGAGG+CGG + chr3_1:82062498-82062517 Msa0380410:CDS 50.0%
AGCCTGACAAGAAGGCAAAC+AGG + chr3_1:82062737-82062756 Msa0380410:CDS 50.0%
GCCTGACAAGAAGGCAAACA+GGG + chr3_1:82062738-82062757 Msa0380410:CDS 50.0%
ACCCTGTTTGCCTTCTTGTC+AGG - chr3_1:82062742-82062761 None:intergenic 50.0%
TATCCCTGTACCGAAGGTGA+CGG + chr3_1:82062765-82062784 Msa0380410:CDS 50.0%
GCAAGCTATGGCACAGAAGA+AGG + chr3_1:82062819-82062838 Msa0380410:CDS 50.0%
AGCTATGGCACAGAAGAAGG+CGG + chr3_1:82062822-82062841 Msa0380410:CDS 50.0%
AAGCGAAGAAGAGGACTGCT+GGG + chr3_1:82062848-82062867 Msa0380410:CDS 50.0%
!! TGTGAATTCTAAGGCCGAGG+CGG + chr3_1:82062498-82062517 Msa0380410:CDS 50.0%
AGCCTGACAAGAAGGCAAAC+AGG + chr3_1:82062737-82062756 Msa0380410:CDS 50.0%
GCCTGACAAGAAGGCAAACA+GGG + chr3_1:82062738-82062757 Msa0380410:CDS 50.0%
ACCCTGTTTGCCTTCTTGTC+AGG - chr3_1:82062742-82062761 None:intergenic 50.0%
TATCCCTGTACCGAAGGTGA+CGG + chr3_1:82062765-82062784 Msa0380410:CDS 50.0%
GCAAGCTATGGCACAGAAGA+AGG + chr3_1:82062819-82062838 Msa0380410:CDS 50.0%
AGCTATGGCACAGAAGAAGG+CGG + chr3_1:82062822-82062841 Msa0380410:CDS 50.0%
AAGCGAAGAAGAGGACTGCT+GGG + chr3_1:82062848-82062867 Msa0380410:CDS 50.0%
AGCAGCAACTGCTGAAGCAG+AGG + chr3_1:82062408-82062427 Msa0380410:five_prime_UTR 55.0%
!! GGGTGTGAATTCTAAGGCCG+AGG + chr3_1:82062495-82062514 Msa0380410:CDS 55.0%
GAGAGAAGGAGGAGCAGTAC+TGG + chr3_1:82062572-82062591 Msa0380410:CDS 55.0%
CTCTTCCTCCCTCTTCTTGG+CGG - chr3_1:82062626-82062645 None:intergenic 55.0%
CAAGAAGAGGGAGGAAGAGG+CGG + chr3_1:82062627-82062646 Msa0380410:CDS 55.0%
GGAGGAAGAGGCGGATAAGA+AGG + chr3_1:82062636-82062655 Msa0380410:CDS 55.0%
GGAAGAGGCGGATAAGAAGG+CGG + chr3_1:82062639-82062658 Msa0380410:CDS 55.0%
GGTGTCTATCCCTGTACCGA+AGG + chr3_1:82062759-82062778 Msa0380410:CDS 55.0%
TATGGCACAGAAGAAGGCGG+AGG + chr3_1:82062825-82062844 Msa0380410:CDS 55.0%
GAAGCGAAGAAGAGGACTGC+TGG + chr3_1:82062847-82062866 Msa0380410:CDS 55.0%
AGCGAAGAAGAGGACTGCTG+GGG + chr3_1:82062849-82062868 Msa0380410:CDS 55.0%
! GATGGCTTCGTCAAGAGTGC+TGG - chr3_1:82062938-82062957 None:intergenic 55.0%
AGCAGCAACTGCTGAAGCAG+AGG + chr3_1:82062408-82062427 Msa0380410:five_prime_UTR 55.0%
!! GGGTGTGAATTCTAAGGCCG+AGG + chr3_1:82062495-82062514 Msa0380410:CDS 55.0%
GAGAGAAGGAGGAGCAGTAC+TGG + chr3_1:82062572-82062591 Msa0380410:CDS 55.0%
CTCTTCCTCCCTCTTCTTGG+CGG - chr3_1:82062626-82062645 None:intergenic 55.0%
CAAGAAGAGGGAGGAAGAGG+CGG + chr3_1:82062627-82062646 Msa0380410:CDS 55.0%
GGAGGAAGAGGCGGATAAGA+AGG + chr3_1:82062636-82062655 Msa0380410:CDS 55.0%
GGAAGAGGCGGATAAGAAGG+CGG + chr3_1:82062639-82062658 Msa0380410:CDS 55.0%
GGTGTCTATCCCTGTACCGA+AGG + chr3_1:82062759-82062778 Msa0380410:CDS 55.0%
TATGGCACAGAAGAAGGCGG+AGG + chr3_1:82062825-82062844 Msa0380410:CDS 55.0%
GAAGCGAAGAAGAGGACTGC+TGG + chr3_1:82062847-82062866 Msa0380410:CDS 55.0%
AGCGAAGAAGAGGACTGCTG+GGG + chr3_1:82062849-82062868 Msa0380410:CDS 55.0%
! GATGGCTTCGTCAAGAGTGC+TGG - chr3_1:82062938-82062957 None:intergenic 55.0%
CCTAGTAGGGTTTCGCTCGC+TGG - chr3_1:82062451-82062470 None:intergenic 60.0%
CCAGCGAGCGAAACCCTACT+AGG + chr3_1:82062448-82062467 Msa0380410:five_prime_UTR 60.0%
CGCCGCGGAAACGGAGAAAA+AGG + chr3_1:82062537-82062556 Msa0380410:CDS 60.0%
GGATCGGGAGACTCGAGAGA+AGG + chr3_1:82062558-82062577 Msa0380410:CDS 60.0%
TCGGGAGACTCGAGAGAAGG+AGG + chr3_1:82062561-82062580 Msa0380410:CDS 60.0%
CGCCAAGAAGAGGGAGGAAG+AGG + chr3_1:82062624-82062643 Msa0380410:CDS 60.0%
CGCCTCTTCCTCCCTCTTCT+TGG - chr3_1:82062629-82062648 None:intergenic 60.0%
AGAGGCGGATAAGAAGGCGG+AGG + chr3_1:82062642-82062661 Msa0380410:CDS 60.0%
GGAGCTGGAGGAGAAAGAGC+TGG + chr3_1:82062699-82062718 Msa0380410:CDS 60.0%
CCTCCGTCACCTTCGGTACA+GGG - chr3_1:82062771-82062790 None:intergenic 60.0%
!! TGTACCGAAGGTGACGGAGG+CGG + chr3_1:82062771-82062790 Msa0380410:CDS 60.0%
GCGAGAGGAGGAGCAAGCTA+TGG + chr3_1:82062807-82062826 Msa0380410:CDS 60.0%
AGGCGGAGGAAGCGAAGAAG+AGG + chr3_1:82062839-82062858 Msa0380410:CDS 60.0%
GAAGAAGAGGACTGCTGGGG+AGG + chr3_1:82062852-82062871 Msa0380410:CDS 60.0%
! GGAGGAGGAGTACGAGAGAG+TGG + chr3_1:82062870-82062889 Msa0380410:CDS 60.0%
GCGCCTCAAGGCTTCCTTCA+AGG + chr3_1:82063008-82063027 Msa0380410:CDS 60.0%
CCTAGTAGGGTTTCGCTCGC+TGG - chr3_1:82062451-82062470 None:intergenic 60.0%
CCAGCGAGCGAAACCCTACT+AGG + chr3_1:82062448-82062467 Msa0380410:five_prime_UTR 60.0%
CGCCGCGGAAACGGAGAAAA+AGG + chr3_1:82062537-82062556 Msa0380410:CDS 60.0%
GGATCGGGAGACTCGAGAGA+AGG + chr3_1:82062558-82062577 Msa0380410:CDS 60.0%
TCGGGAGACTCGAGAGAAGG+AGG + chr3_1:82062561-82062580 Msa0380410:CDS 60.0%
CGCCAAGAAGAGGGAGGAAG+AGG + chr3_1:82062624-82062643 Msa0380410:CDS 60.0%
CGCCTCTTCCTCCCTCTTCT+TGG - chr3_1:82062629-82062648 None:intergenic 60.0%
AGAGGCGGATAAGAAGGCGG+AGG + chr3_1:82062642-82062661 Msa0380410:CDS 60.0%
GGAGCTGGAGGAGAAAGAGC+TGG + chr3_1:82062699-82062718 Msa0380410:CDS 60.0%
CCTCCGTCACCTTCGGTACA+GGG - chr3_1:82062771-82062790 None:intergenic 60.0%
!! TGTACCGAAGGTGACGGAGG+CGG + chr3_1:82062771-82062790 Msa0380410:CDS 60.0%
GCGAGAGGAGGAGCAAGCTA+TGG + chr3_1:82062807-82062826 Msa0380410:CDS 60.0%
AGGCGGAGGAAGCGAAGAAG+AGG + chr3_1:82062839-82062858 Msa0380410:CDS 60.0%
GAAGAAGAGGACTGCTGGGG+AGG + chr3_1:82062852-82062871 Msa0380410:CDS 60.0%
! GGAGGAGGAGTACGAGAGAG+TGG + chr3_1:82062870-82062889 Msa0380410:CDS 60.0%
GCGCCTCAAGGCTTCCTTCA+AGG + chr3_1:82063008-82063027 Msa0380410:CDS 60.0%
!! ATTCTAAGGCCGAGGCGGCG+AGG + chr3_1:82062503-82062522 Msa0380410:CDS 65.0%
!! TTCTAAGGCCGAGGCGGCGA+GGG + chr3_1:82062504-82062523 Msa0380410:CDS 65.0%
!! GGAGGAGCAGTACTGGCGAG+AGG + chr3_1:82062579-82062598 Msa0380410:CDS 65.0%
GGCGGATAAGAAGGCGGAGG+CGG + chr3_1:82062645-82062664 Msa0380410:CDS 65.0%
GGATAAGAAGGCGGAGGCGG+CGG + chr3_1:82062648-82062667 Msa0380410:CDS 65.0%
! ACGAGCGGAGGTGCGTCGAT+TGG + chr3_1:82062675-82062694 Msa0380410:CDS 65.0%
!! AGCGGAGGTGCGTCGATTGG+CGG + chr3_1:82062678-82062697 Msa0380410:CDS 65.0%
! CCCTGTACCGAAGGTGACGG+AGG + chr3_1:82062768-82062787 Msa0380410:CDS 65.0%
GCCTCCGTCACCTTCGGTAC+AGG - chr3_1:82062772-82062791 None:intergenic 65.0%
AGCTCCGCCTCCGTCACCTT+CGG - chr3_1:82062778-82062797 None:intergenic 65.0%
GAAGAGGACTGCTGGGGAGG+AGG + chr3_1:82062855-82062874 Msa0380410:CDS 65.0%
CTCTCTGGATGCCTGTCGGG+AGG - chr3_1:82062988-82063007 None:intergenic 65.0%
CTCCCGACAGGCATCCAGAG+AGG + chr3_1:82062986-82063005 Msa0380410:CDS 65.0%
CGCCTCTCTGGATGCCTGTC+GGG - chr3_1:82062991-82063010 None:intergenic 65.0%
GCGCCTCTCTGGATGCCTGT+CGG - chr3_1:82062992-82063011 None:intergenic 65.0%
!! GCATCCAGAGAGGCGCCTCA+AGG + chr3_1:82062996-82063015 Msa0380410:CDS 65.0%
! GAAGCCTTGAGGCGCCTCTC+TGG - chr3_1:82063003-82063022 None:intergenic 65.0%
!! ATTCTAAGGCCGAGGCGGCG+AGG + chr3_1:82062503-82062522 Msa0380410:CDS 65.0%
!! TTCTAAGGCCGAGGCGGCGA+GGG + chr3_1:82062504-82062523 Msa0380410:CDS 65.0%
!! GGAGGAGCAGTACTGGCGAG+AGG + chr3_1:82062579-82062598 Msa0380410:CDS 65.0%
GGCGGATAAGAAGGCGGAGG+CGG + chr3_1:82062645-82062664 Msa0380410:CDS 65.0%
GGATAAGAAGGCGGAGGCGG+CGG + chr3_1:82062648-82062667 Msa0380410:CDS 65.0%
! ACGAGCGGAGGTGCGTCGAT+TGG + chr3_1:82062675-82062694 Msa0380410:CDS 65.0%
!! AGCGGAGGTGCGTCGATTGG+CGG + chr3_1:82062678-82062697 Msa0380410:CDS 65.0%
! CCCTGTACCGAAGGTGACGG+AGG + chr3_1:82062768-82062787 Msa0380410:CDS 65.0%
GCCTCCGTCACCTTCGGTAC+AGG - chr3_1:82062772-82062791 None:intergenic 65.0%
AGCTCCGCCTCCGTCACCTT+CGG - chr3_1:82062778-82062797 None:intergenic 65.0%
GAAGAGGACTGCTGGGGAGG+AGG + chr3_1:82062855-82062874 Msa0380410:CDS 65.0%
CTCTCTGGATGCCTGTCGGG+AGG - chr3_1:82062988-82063007 None:intergenic 65.0%
CTCCCGACAGGCATCCAGAG+AGG + chr3_1:82062986-82063005 Msa0380410:CDS 65.0%
CGCCTCTCTGGATGCCTGTC+GGG - chr3_1:82062991-82063010 None:intergenic 65.0%
GCGCCTCTCTGGATGCCTGT+CGG - chr3_1:82062992-82063011 None:intergenic 65.0%
!! GCATCCAGAGAGGCGCCTCA+AGG + chr3_1:82062996-82063015 Msa0380410:CDS 65.0%
! GAAGCCTTGAGGCGCCTCTC+TGG - chr3_1:82063003-82063022 None:intergenic 65.0%
CGTTTCCGCGGCGCTCTTGC+GGG - chr3_1:82062530-82062549 None:intergenic 70.0%
CCGTTTCCGCGGCGCTCTTG+CGG - chr3_1:82062531-82062550 None:intergenic 70.0%
!! CCGCAAGAGCGCCGCGGAAA+CGG + chr3_1:82062528-82062547 Msa0380410:CDS 70.0%
AGCCACGCGCCGCCAAGAAG+AGG + chr3_1:82062614-82062633 Msa0380410:CDS 70.0%
GCCACGCGCCGCCAAGAAGA+GGG + chr3_1:82062615-82062634 Msa0380410:CDS 70.0%
TCCCTCTTCTTGGCGGCGCG+TGG - chr3_1:82062619-82062638 None:intergenic 70.0%
ACGCGCCGCCAAGAAGAGGG+AGG + chr3_1:82062618-82062637 Msa0380410:CDS 70.0%
!! GGTGCGTCGATTGGCGGAGC+TGG + chr3_1:82062684-82062703 Msa0380410:CDS 70.0%
!! GCGTCGATTGGCGGAGCTGG+AGG + chr3_1:82062687-82062706 Msa0380410:CDS 70.0%
!! AGGTGACGGAGGCGGAGCTG+AGG + chr3_1:82062779-82062798 Msa0380410:CDS 70.0%
CGGAGGCGGAGCTGAGGAAG+CGG + chr3_1:82062785-82062804 Msa0380410:CDS 70.0%
GGAGCTGAGGAAGCGGCGAG+AGG + chr3_1:82062792-82062811 Msa0380410:CDS 70.0%
GCTGAGGAAGCGGCGAGAGG+AGG + chr3_1:82062795-82062814 Msa0380410:CDS 70.0%
CGTTTCCGCGGCGCTCTTGC+GGG - chr3_1:82062530-82062549 None:intergenic 70.0%
CCGTTTCCGCGGCGCTCTTG+CGG - chr3_1:82062531-82062550 None:intergenic 70.0%
!! CCGCAAGAGCGCCGCGGAAA+CGG + chr3_1:82062528-82062547 Msa0380410:CDS 70.0%
AGCCACGCGCCGCCAAGAAG+AGG + chr3_1:82062614-82062633 Msa0380410:CDS 70.0%
GCCACGCGCCGCCAAGAAGA+GGG + chr3_1:82062615-82062634 Msa0380410:CDS 70.0%
TCCCTCTTCTTGGCGGCGCG+TGG - chr3_1:82062619-82062638 None:intergenic 70.0%
ACGCGCCGCCAAGAAGAGGG+AGG + chr3_1:82062618-82062637 Msa0380410:CDS 70.0%
!! GGTGCGTCGATTGGCGGAGC+TGG + chr3_1:82062684-82062703 Msa0380410:CDS 70.0%
!! GCGTCGATTGGCGGAGCTGG+AGG + chr3_1:82062687-82062706 Msa0380410:CDS 70.0%
!! AGGTGACGGAGGCGGAGCTG+AGG + chr3_1:82062779-82062798 Msa0380410:CDS 70.0%
CGGAGGCGGAGCTGAGGAAG+CGG + chr3_1:82062785-82062804 Msa0380410:CDS 70.0%
GGAGCTGAGGAAGCGGCGAG+AGG + chr3_1:82062792-82062811 Msa0380410:CDS 70.0%
GCTGAGGAAGCGGCGAGAGG+AGG + chr3_1:82062795-82062814 Msa0380410:CDS 70.0%
! CTTGCGGGCCCTCGCCGCCT+CGG - chr3_1:82062515-82062534 None:intergenic 80.0%
!!! GAGGGCCCGCAAGAGCGCCG+CGG + chr3_1:82062522-82062541 Msa0380410:CDS 80.0%
! GGAGGCGGCGGCACGACGAG+CGG + chr3_1:82062660-82062679 Msa0380410:CDS 80.0%
! CTTGCGGGCCCTCGCCGCCT+CGG - chr3_1:82062515-82062534 None:intergenic 80.0%
!!! GAGGGCCCGCAAGAGCGCCG+CGG + chr3_1:82062522-82062541 Msa0380410:CDS 80.0%
! GGAGGCGGCGGCACGACGAG+CGG + chr3_1:82062660-82062679 Msa0380410:CDS 80.0%
! GGCGGCGGCACGACGAGCGG+AGG + chr3_1:82062663-82062682 Msa0380410:CDS 85.0%
! GGCGGCGGCACGACGAGCGG+AGG + chr3_1:82062663-82062682 Msa0380410:CDS 85.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 82062389 82063644 82062389 ID=Msa0380410;Name=Msa0380410
chr3_1 mRNA 82062389 82063644 82062389 ID=Msa0380410-mRNA-1;Parent=Msa0380410;Name=Msa0380410-mRNA-1;_AED=0.02;_eAED=0.02;_QI=92|1|1|1|1|1|3|311|227
chr3_1 exon 82062389 82063029 82062389 ID=Msa0380410-mRNA-1:exon:20863;Parent=Msa0380410-mRNA-1
chr3_1 exon 82063109 82063228 82063109 ID=Msa0380410-mRNA-1:exon:20864;Parent=Msa0380410-mRNA-1
chr3_1 exon 82063319 82063644 82063319 ID=Msa0380410-mRNA-1:exon:20865;Parent=Msa0380410-mRNA-1
chr3_1 five_prime_UTR 82062389 82062480 82062389 ID=Msa0380410-mRNA-1:five_prime_utr;Parent=Msa0380410-mRNA-1
chr3_1 CDS 82062481 82063029 82062481 ID=Msa0380410-mRNA-1:cds;Parent=Msa0380410-mRNA-1
chr3_1 CDS 82063109 82063228 82063109 ID=Msa0380410-mRNA-1:cds;Parent=Msa0380410-mRNA-1
chr3_1 CDS 82063319 82063333 82063319 ID=Msa0380410-mRNA-1:cds;Parent=Msa0380410-mRNA-1
chr3_1 three_prime_UTR 82063334 82063644 82063334 ID=Msa0380410-mRNA-1:three_prime_utr;Parent=Msa0380410-mRNA-1
Gene Sequence

>Msa0380410

ATGCCCAAAAAGATGGGTGTGAATTCTAAGGCCGAGGCGGCGAGGGCCCGCAAGAGCGCCGCGGAAACGGAGAAAAAGGATCGGGAGACTCGAGAGAAGGAGGAGCAGTACTGGCGAGAGGCAGAGAGTTCAAAGCCACGCGCCGCCAAGAAGAGGGAGGAAGAGGCGGATAAGAAGGCGGAGGCGGCGGCACGACGAGCGGAGGTGCGTCGATTGGCGGAGCTGGAGGAGAAAGAGCTGGAGAAGATGATTAAAAAGCCTGACAAGAAGGCAAACAGGGTGTCTATCCCTGTACCGAAGGTGACGGAGGCGGAGCTGAGGAAGCGGCGAGAGGAGGAGCAAGCTATGGCACAGAAGAAGGCGGAGGAAGCGAAGAAGAGGACTGCTGGGGAGGAGGAGTACGAGAGAGTGGTGCTTGTGTCGAATACGAATCGTGATGATTCAATTATCGAAGCCAGCACTCTTGACGAAGCCATCGCTCAGATGAGTATCGATAACAACTTACCTCCCGACAGGCATCCAGAGAGGCGCCTCAAGGCTTCCTTCAAGGCATTTGAAGAAGCTGAACTACCTAAGTTGAAGCAAGAGAAACCAGGGCTTACATACACACAGTACAAGGACATGATTTGGAAGCTTTGGAAGAAATCTCCTGACAATCCTCTTAATCAAATTGCTGCCGAGTGA
Protein sequence

>Msa0380410

MPKKMGVNSKAEAARARKSAAETEKKDRETREKEEQYWREAESSKPRAAKKREEEADKKAEAAARRAEVRRLAELEEKELEKMIKKPDKKANRVSIPVPKVTEAELRKRREEEQAMAQKKAEEAKKRTAGEEEYERVVLVSNTNRDDSIIEASTLDEAIAQMSIDNNLPPDRHPERRLKASFKAFEEAELPKLKQEKPGLTYTQYKDMIWKLWKKSPDNPLNQIAAE*