AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0476160

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0476160 MtrunA17_Chr3g0145951 99.119 227 2 0 1 227 1 227 1.98e-158 437
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0476160 AT1G16210.1 73.009 226 58 1 1 223 1 226 1.16e-106 307

Find 97 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCGAGTGTATTTAAAATT+TGG 0.164169 3_3:+79931056 None:intergenic
CGCCTCTTCCTCCCTCTTCT+TGG 0.214378 3_3:+79932026 None:intergenic
AAGACAACTTCAAAGATTAT+TGG 0.247121 3_3:+79931107 None:intergenic
CGTTTCCGCGGCGCTCTTGC+GGG 0.269884 3_3:+79932125 None:intergenic
CGCCGCGGAAACGGAGAAAA+AGG 0.310222 3_3:-79932115 Msa0476160:CDS
AGGACATGATTTGGAAGCTT+TGG 0.331210 3_3:-79931477 Msa0476160:CDS
GCATCTTTAATTCACCTAGT+AGG 0.334052 3_3:+79932190 None:intergenic
ACCCTGTTTGCCTTCTTGTC+AGG 0.352573 3_3:+79931913 None:intergenic
GGTTTCTCTTGCTTCAACTT+AGG 0.359376 3_3:+79931523 None:intergenic
CACAGTACAAGGACATGATT+TGG 0.361884 3_3:-79931486 Msa0476160:CDS
CGCCTCTCTGGATGCCTGTC+GGG 0.367827 3_3:+79931664 None:intergenic
CGCCAAGAAGAGGGAGGAAG+AGG 0.386559 3_3:-79932028 Msa0476160:CDS
CCGCAAGAGCGCCGCGGAAA+CGG 0.388342 3_3:-79932124 Msa0476160:CDS
GAAGCCTTGAGGCGCCTCTC+TGG 0.388885 3_3:+79931652 None:intergenic
GAGTGAGTGACTTGATTAAG+AGG 0.390157 3_3:+79931436 None:intergenic
CCTAGTAGGGTTTCGCTCGC+TGG 0.400470 3_3:+79932204 None:intergenic
GCCTCCGTCACCTTCGGTAC+AGG 0.404250 3_3:+79931883 None:intergenic
GGTGCGTCGATTGGCGGAGC+TGG 0.407431 3_3:-79931968 Msa0476160:CDS
CAAAGATTATTGGTTGTAGT+AGG 0.408363 3_3:+79931117 None:intergenic
CCGTTTCCGCGGCGCTCTTG+CGG 0.414275 3_3:+79932124 None:intergenic
CATCTTTAATTCACCTAGTA+GGG 0.414363 3_3:+79932191 None:intergenic
AGCCTGACAAGAAGGCAAAC+AGG 0.415814 3_3:-79931915 Msa0476160:CDS
AAAGATGGGTGTGAATTCTA+AGG 0.416000 3_3:-79932163 Msa0476160:CDS
GGAGGAAGAGGCGGATAAGA+AGG 0.418038 3_3:-79932016 Msa0476160:CDS
CTTGCGGGCCCTCGCCGCCT+CGG 0.418040 3_3:+79932140 None:intergenic
GGATAAGAAGGCGGAGGCGG+CGG 0.424210 3_3:-79932004 Msa0476160:CDS
GAGAGAAGGAGGAGCAGTAC+TGG 0.439791 3_3:-79932080 Msa0476160:CDS
ACTTGATTAAGAGGATTGTC+AGG 0.442090 3_3:+79931445 None:intergenic
GCAAGCTATGGCACAGAAGA+AGG 0.443447 3_3:-79931833 Msa0476160:CDS
GGCGGATAAGAAGGCGGAGG+CGG 0.454208 3_3:-79932007 Msa0476160:CDS
GAAGAGGACTGCTGGGGAGG+AGG 0.460637 3_3:-79931797 Msa0476160:CDS
TTCTAAGGCCGAGGCGGCGA+GGG 0.465736 3_3:-79932148 Msa0476160:CDS
GAAGCGAAGAAGAGGACTGC+TGG 0.465817 3_3:-79931805 Msa0476160:CDS
GCGCCTCAAGGCTTCCTTCA+AGG 0.472754 3_3:-79931644 Msa0476160:intron
AGCGGAGGTGCGTCGATTGG+CGG 0.475781 3_3:-79931974 Msa0476160:CDS
AGCTCCGCCTCCGTCACCTT+CGG 0.476514 3_3:+79931877 None:intergenic
AAAACAGATACATACCTTGA+AGG 0.481347 3_3:+79931630 None:intergenic
GCGCCTCTCTGGATGCCTGT+CGG 0.482921 3_3:+79931663 None:intergenic
TACTGTGTGTATGTAAGCCC+TGG 0.485764 3_3:+79931502 None:intergenic
GGAGGCGGCGGCACGACGAG+CGG 0.491424 3_3:-79931992 Msa0476160:CDS
AAGCGAAGAAGAGGACTGCT+GGG 0.493135 3_3:-79931804 Msa0476160:CDS
GATGGCTTCGTCAAGAGTGC+TGG 0.493876 3_3:+79931717 None:intergenic
CGGAGGCGGAGCTGAGGAAG+CGG 0.494922 3_3:-79931867 Msa0476160:CDS
CCAGCGAGCGAAACCCTACT+AGG 0.505208 3_3:-79932204 Msa0476160:five_prime_UTR
GCTGAGGAAGCGGCGAGAGG+AGG 0.505549 3_3:-79931857 Msa0476160:CDS
TCCCTCTTCTTGGCGGCGCG+TGG 0.507668 3_3:+79932036 None:intergenic
CTCTTCCTCCCTCTTCTTGG+CGG 0.507806 3_3:+79932029 None:intergenic
AGATTATTGGTTGTAGTAGG+AGG 0.510390 3_3:+79931120 None:intergenic
GCCACGCGCCGCCAAGAAGA+GGG 0.513629 3_3:-79932037 Msa0476160:CDS
AGCCACGCGCCGCCAAGAAG+AGG 0.516989 3_3:-79932038 Msa0476160:CDS
GCGAGAGGAGGAGCAAGCTA+TGG 0.521774 3_3:-79931845 Msa0476160:CDS
ACGAGCGGAGGTGCGTCGAT+TGG 0.522062 3_3:-79931977 Msa0476160:CDS
GGCGGCGGCACGACGAGCGG+AGG 0.533711 3_3:-79931989 Msa0476160:CDS
ACTTAATCATGAATTTGAGA+TGG 0.534693 3_3:+79931269 None:intergenic
CCTCCGTCACCTTCGGTACA+GGG 0.541244 3_3:+79931884 None:intergenic
GGAGCTGAGGAAGCGGCGAG+AGG 0.552884 3_3:-79931860 Msa0476160:CDS
GCCTGACAAGAAGGCAAACA+GGG 0.555319 3_3:-79931914 Msa0476160:CDS
GCTTACATACACACAGTACA+AGG 0.555636 3_3:-79931497 Msa0476160:CDS
CAAGAAGAGGGAGGAAGAGG+CGG 0.555830 3_3:-79932025 Msa0476160:CDS
GGGTGTGAATTCTAAGGCCG+AGG 0.555835 3_3:-79932157 Msa0476160:CDS
GAAGAAGAGGACTGCTGGGG+AGG 0.559520 3_3:-79931800 Msa0476160:CDS
AGGTGACGGAGGCGGAGCTG+AGG 0.562961 3_3:-79931873 Msa0476160:CDS
AGAGGCGGATAAGAAGGCGG+AGG 0.563473 3_3:-79932010 Msa0476160:CDS
TGTACCGAAGGTGACGGAGG+CGG 0.565468 3_3:-79931881 Msa0476160:CDS
AGGCGGAGGAAGCGAAGAAG+AGG 0.566562 3_3:-79931813 Msa0476160:CDS
GATTCAATAAAGTCATGGCT+TGG 0.566810 3_3:-79931314 Msa0476160:three_prime_UTR
CTCTCTGGATGCCTGTCGGG+AGG 0.568049 3_3:+79931667 None:intergenic
GCGTCGATTGGCGGAGCTGG+AGG 0.569855 3_3:-79931965 Msa0476160:CDS
GCATCCAGAGAGGCGCCTCA+AGG 0.574178 3_3:-79931656 Msa0476160:CDS
ATCGATACTCATCTGAGCGA+TGG 0.576376 3_3:+79931699 None:intergenic
AGCAGCAACTGCTGAAGCAG+AGG 0.579802 3_3:-79932244 Msa0476160:five_prime_UTR
ATTCTAAGGCCGAGGCGGCG+AGG 0.588501 3_3:-79932149 Msa0476160:CDS
TCGGGAGACTCGAGAGAAGG+AGG 0.591271 3_3:-79932091 Msa0476160:CDS
TCAATAAAGTCATGGCTTGG+CGG 0.593024 3_3:-79931311 Msa0476160:three_prime_UTR
ATAACAACTTACCTCCCGAC+AGG 0.598312 3_3:-79931678 Msa0476160:CDS
ACGCGCCGCCAAGAAGAGGG+AGG 0.598349 3_3:-79932034 Msa0476160:CDS
GAGGGCCCGCAAGAGCGCCG+CGG 0.599591 3_3:-79932130 Msa0476160:CDS
GGAGCTGGAGGAGAAAGAGC+TGG 0.599937 3_3:-79931953 Msa0476160:CDS
AAGTTGAAGCAAGAGAAACC+AGG 0.604189 3_3:-79931520 Msa0476160:CDS
GGATCGGGAGACTCGAGAGA+AGG 0.606508 3_3:-79932094 Msa0476160:CDS
CCCTGTACCGAAGGTGACGG+AGG 0.608676 3_3:-79931884 Msa0476160:CDS
TATGGCACAGAAGAAGGCGG+AGG 0.610578 3_3:-79931827 Msa0476160:CDS
ATGATGATTCAATAAAGTCA+TGG 0.612785 3_3:-79931319 Msa0476160:three_prime_UTR
GGAGGAGGAGTACGAGAGAG+TGG 0.614209 3_3:-79931782 Msa0476160:CDS
GGTGTCTATCCCTGTACCGA+AGG 0.615935 3_3:-79931893 Msa0476160:CDS
TTATTGAATCATCATTCACT+CGG 0.617967 3_3:+79931327 None:intergenic
GGAAGAGGCGGATAAGAAGG+CGG 0.620199 3_3:-79932013 Msa0476160:CDS
TATCCCTGTACCGAAGGTGA+CGG 0.630098 3_3:-79931887 Msa0476160:CDS
CTCCCGACAGGCATCCAGAG+AGG 0.637354 3_3:-79931666 Msa0476160:CDS
GGAGGAGCAGTACTGGCGAG+AGG 0.649799 3_3:-79932073 Msa0476160:CDS
TCATATACTACTGCTTACAG+AGG 0.659085 3_3:+79931212 None:intergenic
TGTGAATTCTAAGGCCGAGG+CGG 0.660597 3_3:-79932154 Msa0476160:CDS
AGTTGAAGCAAGAGAAACCA+GGG 0.661223 3_3:-79931519 Msa0476160:CDS
CATATACTACTGCTTACAGA+GGG 0.665172 3_3:+79931213 None:intergenic
AGCGAAGAAGAGGACTGCTG+GGG 0.681467 3_3:-79931803 Msa0476160:CDS
ATACCTTGAAGGAAGCCTTG+AGG 0.684866 3_3:+79931641 None:intergenic
AGCTATGGCACAGAAGAAGG+CGG 0.695332 3_3:-79931830 Msa0476160:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATAACAAAAATAAAAAGAT+AGG + chr3_3:79931912-79931931 None:intergenic 15.0%
!! GATAACAAAAATAAAAAGAT+AGG + chr3_3:79931912-79931931 None:intergenic 15.0%
!!! TATCGAGTGTATTTAAAATT+TGG + chr3_3:79932241-79932260 None:intergenic 20.0%
!!! TATCGAGTGTATTTAAAATT+TGG + chr3_3:79932241-79932260 None:intergenic 20.0%
! AATTCATTCATTCATTCATC+AGG - chr3_3:79931728-79931747 Msa0476160:CDS 25.0%
! ATAAAAAGATAGGCTAAAAG+TGG + chr3_3:79931902-79931921 None:intergenic 25.0%
! TTATTGAATCATCATTCACT+CGG + chr3_3:79931970-79931989 None:intergenic 25.0%
! ATGATGATTCAATAAAGTCA+TGG - chr3_3:79931975-79931994 Msa0476160:CDS 25.0%
! ACTTAATCATGAATTTGAGA+TGG + chr3_3:79932028-79932047 None:intergenic 25.0%
!!! ACAAACATTGTTTGTTTTCA+TGG - chr3_3:79932134-79932153 Msa0476160:CDS 25.0%
! AAGACAACTTCAAAGATTAT+TGG + chr3_3:79932190-79932209 None:intergenic 25.0%
! AATTCATTCATTCATTCATC+AGG - chr3_3:79931728-79931747 Msa0476160:CDS 25.0%
! ATAAAAAGATAGGCTAAAAG+TGG + chr3_3:79931902-79931921 None:intergenic 25.0%
! TTATTGAATCATCATTCACT+CGG + chr3_3:79931970-79931989 None:intergenic 25.0%
! ATGATGATTCAATAAAGTCA+TGG - chr3_3:79931975-79931994 Msa0476160:CDS 25.0%
! ACTTAATCATGAATTTGAGA+TGG + chr3_3:79932028-79932047 None:intergenic 25.0%
!!! ACAAACATTGTTTGTTTTCA+TGG - chr3_3:79932134-79932153 Msa0476160:CDS 25.0%
! AAGACAACTTCAAAGATTAT+TGG + chr3_3:79932190-79932209 None:intergenic 25.0%
CATCTTTAATTCACCTAGTA+GGG + chr3_3:79931106-79931125 None:intergenic 30.0%
ATTAAAGATGCCCAAAAAGA+TGG - chr3_3:79931116-79931135 Msa0476160:three_prime_UTR 30.0%
TTAAAGATGCCCAAAAAGAT+GGG - chr3_3:79931117-79931136 Msa0476160:three_prime_UTR 30.0%
AAAACAGATACATACCTTGA+AGG + chr3_3:79931667-79931686 None:intergenic 30.0%
!! CTAAAAGTGGATTATAAAGG+AGG + chr3_3:79931889-79931908 None:intergenic 30.0%
!! AGGCTAAAAGTGGATTATAA+AGG + chr3_3:79931892-79931911 None:intergenic 30.0%
! CAAAGATTATTGGTTGTAGT+AGG + chr3_3:79932180-79932199 None:intergenic 30.0%
CATCTTTAATTCACCTAGTA+GGG + chr3_3:79931106-79931125 None:intergenic 30.0%
ATTAAAGATGCCCAAAAAGA+TGG - chr3_3:79931116-79931135 Msa0476160:three_prime_UTR 30.0%
TTAAAGATGCCCAAAAAGAT+GGG - chr3_3:79931117-79931136 Msa0476160:three_prime_UTR 30.0%
AAAACAGATACATACCTTGA+AGG + chr3_3:79931667-79931686 None:intergenic 30.0%
!! CTAAAAGTGGATTATAAAGG+AGG + chr3_3:79931889-79931908 None:intergenic 30.0%
!! AGGCTAAAAGTGGATTATAA+AGG + chr3_3:79931892-79931911 None:intergenic 30.0%
! CAAAGATTATTGGTTGTAGT+AGG + chr3_3:79932180-79932199 None:intergenic 30.0%
GCATCTTTAATTCACCTAGT+AGG + chr3_3:79931107-79931126 None:intergenic 35.0%
! GAATTCACACCCATCTTTTT+GGG + chr3_3:79931129-79931148 None:intergenic 35.0%
! AGAATTCACACCCATCTTTT+TGG + chr3_3:79931130-79931149 None:intergenic 35.0%
AAAGATGGGTGTGAATTCTA+AGG - chr3_3:79931131-79931150 Msa0476160:three_prime_UTR 35.0%
GATTAAAAAGCCTGACAAGA+AGG - chr3_3:79931371-79931390 Msa0476160:intron 35.0%
ACTTGATTAAGAGGATTGTC+AGG + chr3_3:79931852-79931871 None:intergenic 35.0%
GATTCAATAAAGTCATGGCT+TGG - chr3_3:79931980-79931999 Msa0476160:CDS 35.0%
CATATACTACTGCTTACAGA+GGG + chr3_3:79932084-79932103 None:intergenic 35.0%
TCATATACTACTGCTTACAG+AGG + chr3_3:79932085-79932104 None:intergenic 35.0%
! AGATTATTGGTTGTAGTAGG+AGG + chr3_3:79932177-79932196 None:intergenic 35.0%
GCATCTTTAATTCACCTAGT+AGG + chr3_3:79931107-79931126 None:intergenic 35.0%
! GAATTCACACCCATCTTTTT+GGG + chr3_3:79931129-79931148 None:intergenic 35.0%
! AGAATTCACACCCATCTTTT+TGG + chr3_3:79931130-79931149 None:intergenic 35.0%
AAAGATGGGTGTGAATTCTA+AGG - chr3_3:79931131-79931150 Msa0476160:three_prime_UTR 35.0%
GATTAAAAAGCCTGACAAGA+AGG - chr3_3:79931371-79931390 Msa0476160:intron 35.0%
ACTTGATTAAGAGGATTGTC+AGG + chr3_3:79931852-79931871 None:intergenic 35.0%
GATTCAATAAAGTCATGGCT+TGG - chr3_3:79931980-79931999 Msa0476160:CDS 35.0%
CATATACTACTGCTTACAGA+GGG + chr3_3:79932084-79932103 None:intergenic 35.0%
TCATATACTACTGCTTACAG+AGG + chr3_3:79932085-79932104 None:intergenic 35.0%
! AGATTATTGGTTGTAGTAGG+AGG + chr3_3:79932177-79932196 None:intergenic 35.0%
GGTTTCTCTTGCTTCAACTT+AGG + chr3_3:79931774-79931793 None:intergenic 40.0%
AAGTTGAAGCAAGAGAAACC+AGG - chr3_3:79931774-79931793 Msa0476160:CDS 40.0%
AGTTGAAGCAAGAGAAACCA+GGG - chr3_3:79931775-79931794 Msa0476160:CDS 40.0%
GCTTACATACACACAGTACA+AGG - chr3_3:79931797-79931816 Msa0476160:CDS 40.0%
CACAGTACAAGGACATGATT+TGG - chr3_3:79931808-79931827 Msa0476160:CDS 40.0%
! AGGACATGATTTGGAAGCTT+TGG - chr3_3:79931817-79931836 Msa0476160:CDS 40.0%
GAGTGAGTGACTTGATTAAG+AGG + chr3_3:79931861-79931880 None:intergenic 40.0%
! TCAATAAAGTCATGGCTTGG+CGG - chr3_3:79931983-79932002 Msa0476160:CDS 40.0%
GGTTTCTCTTGCTTCAACTT+AGG + chr3_3:79931774-79931793 None:intergenic 40.0%
AAGTTGAAGCAAGAGAAACC+AGG - chr3_3:79931774-79931793 Msa0476160:CDS 40.0%
AGTTGAAGCAAGAGAAACCA+GGG - chr3_3:79931775-79931794 Msa0476160:CDS 40.0%
GCTTACATACACACAGTACA+AGG - chr3_3:79931797-79931816 Msa0476160:CDS 40.0%
CACAGTACAAGGACATGATT+TGG - chr3_3:79931808-79931827 Msa0476160:CDS 40.0%
! AGGACATGATTTGGAAGCTT+TGG - chr3_3:79931817-79931836 Msa0476160:CDS 40.0%
GAGTGAGTGACTTGATTAAG+AGG + chr3_3:79931861-79931880 None:intergenic 40.0%
! TCAATAAAGTCATGGCTTGG+CGG - chr3_3:79931983-79932002 Msa0476160:CDS 40.0%
! ATCCTTTTTCTCCGTTTCCG+CGG + chr3_3:79931184-79931203 None:intergenic 45.0%
CGGAAACGGAGAAAAAGGAT+CGG - chr3_3:79931184-79931203 Msa0476160:three_prime_UTR 45.0%
GGAAACGGAGAAAAAGGATC+GGG - chr3_3:79931185-79931204 Msa0476160:three_prime_UTR 45.0%
ATCGATACTCATCTGAGCGA+TGG + chr3_3:79931598-79931617 None:intergenic 45.0%
ATAACAACTTACCTCCCGAC+AGG - chr3_3:79931616-79931635 Msa0476160:intron 45.0%
ATACCTTGAAGGAAGCCTTG+AGG + chr3_3:79931656-79931675 None:intergenic 45.0%
TACTGTGTGTATGTAAGCCC+TGG + chr3_3:79931795-79931814 None:intergenic 45.0%
! ATCCTTTTTCTCCGTTTCCG+CGG + chr3_3:79931184-79931203 None:intergenic 45.0%
CGGAAACGGAGAAAAAGGAT+CGG - chr3_3:79931184-79931203 Msa0476160:three_prime_UTR 45.0%
GGAAACGGAGAAAAAGGATC+GGG - chr3_3:79931185-79931204 Msa0476160:three_prime_UTR 45.0%
ATCGATACTCATCTGAGCGA+TGG + chr3_3:79931598-79931617 None:intergenic 45.0%
ATAACAACTTACCTCCCGAC+AGG - chr3_3:79931616-79931635 Msa0476160:intron 45.0%
ATACCTTGAAGGAAGCCTTG+AGG + chr3_3:79931656-79931675 None:intergenic 45.0%
TACTGTGTGTATGTAAGCCC+TGG + chr3_3:79931795-79931814 None:intergenic 45.0%
!! TGTGAATTCTAAGGCCGAGG+CGG - chr3_3:79931140-79931159 Msa0476160:three_prime_UTR 50.0%
AGCCTGACAAGAAGGCAAAC+AGG - chr3_3:79931379-79931398 Msa0476160:intron 50.0%
GCCTGACAAGAAGGCAAACA+GGG - chr3_3:79931380-79931399 Msa0476160:intron 50.0%
ACCCTGTTTGCCTTCTTGTC+AGG + chr3_3:79931384-79931403 None:intergenic 50.0%
TATCCCTGTACCGAAGGTGA+CGG - chr3_3:79931407-79931426 Msa0476160:intron 50.0%
GCAAGCTATGGCACAGAAGA+AGG - chr3_3:79931461-79931480 Msa0476160:CDS 50.0%
AGCTATGGCACAGAAGAAGG+CGG - chr3_3:79931464-79931483 Msa0476160:CDS 50.0%
AAGCGAAGAAGAGGACTGCT+GGG - chr3_3:79931490-79931509 Msa0476160:CDS 50.0%
!! TGTGAATTCTAAGGCCGAGG+CGG - chr3_3:79931140-79931159 Msa0476160:three_prime_UTR 50.0%
AGCCTGACAAGAAGGCAAAC+AGG - chr3_3:79931379-79931398 Msa0476160:intron 50.0%
GCCTGACAAGAAGGCAAACA+GGG - chr3_3:79931380-79931399 Msa0476160:intron 50.0%
ACCCTGTTTGCCTTCTTGTC+AGG + chr3_3:79931384-79931403 None:intergenic 50.0%
TATCCCTGTACCGAAGGTGA+CGG - chr3_3:79931407-79931426 Msa0476160:intron 50.0%
GCAAGCTATGGCACAGAAGA+AGG - chr3_3:79931461-79931480 Msa0476160:CDS 50.0%
AGCTATGGCACAGAAGAAGG+CGG - chr3_3:79931464-79931483 Msa0476160:CDS 50.0%
AAGCGAAGAAGAGGACTGCT+GGG - chr3_3:79931490-79931509 Msa0476160:CDS 50.0%
AGCAGCAACTGCTGAAGCAG+AGG - chr3_3:79931050-79931069 Msa0476160:three_prime_UTR 55.0%
!! GGGTGTGAATTCTAAGGCCG+AGG - chr3_3:79931137-79931156 Msa0476160:three_prime_UTR 55.0%
GAGAGAAGGAGGAGCAGTAC+TGG - chr3_3:79931214-79931233 Msa0476160:three_prime_UTR 55.0%
CTCTTCCTCCCTCTTCTTGG+CGG + chr3_3:79931268-79931287 None:intergenic 55.0%
CAAGAAGAGGGAGGAAGAGG+CGG - chr3_3:79931269-79931288 Msa0476160:three_prime_UTR 55.0%
GGAGGAAGAGGCGGATAAGA+AGG - chr3_3:79931278-79931297 Msa0476160:three_prime_UTR 55.0%
GGAAGAGGCGGATAAGAAGG+CGG - chr3_3:79931281-79931300 Msa0476160:three_prime_UTR 55.0%
GGTGTCTATCCCTGTACCGA+AGG - chr3_3:79931401-79931420 Msa0476160:intron 55.0%
TATGGCACAGAAGAAGGCGG+AGG - chr3_3:79931467-79931486 Msa0476160:CDS 55.0%
GAAGCGAAGAAGAGGACTGC+TGG - chr3_3:79931489-79931508 Msa0476160:CDS 55.0%
AGCGAAGAAGAGGACTGCTG+GGG - chr3_3:79931491-79931510 Msa0476160:CDS 55.0%
! GATGGCTTCGTCAAGAGTGC+TGG + chr3_3:79931580-79931599 None:intergenic 55.0%
AGCAGCAACTGCTGAAGCAG+AGG - chr3_3:79931050-79931069 Msa0476160:three_prime_UTR 55.0%
!! GGGTGTGAATTCTAAGGCCG+AGG - chr3_3:79931137-79931156 Msa0476160:three_prime_UTR 55.0%
GAGAGAAGGAGGAGCAGTAC+TGG - chr3_3:79931214-79931233 Msa0476160:three_prime_UTR 55.0%
CTCTTCCTCCCTCTTCTTGG+CGG + chr3_3:79931268-79931287 None:intergenic 55.0%
CAAGAAGAGGGAGGAAGAGG+CGG - chr3_3:79931269-79931288 Msa0476160:three_prime_UTR 55.0%
GGAGGAAGAGGCGGATAAGA+AGG - chr3_3:79931278-79931297 Msa0476160:three_prime_UTR 55.0%
GGAAGAGGCGGATAAGAAGG+CGG - chr3_3:79931281-79931300 Msa0476160:three_prime_UTR 55.0%
GGTGTCTATCCCTGTACCGA+AGG - chr3_3:79931401-79931420 Msa0476160:intron 55.0%
TATGGCACAGAAGAAGGCGG+AGG - chr3_3:79931467-79931486 Msa0476160:CDS 55.0%
GAAGCGAAGAAGAGGACTGC+TGG - chr3_3:79931489-79931508 Msa0476160:CDS 55.0%
AGCGAAGAAGAGGACTGCTG+GGG - chr3_3:79931491-79931510 Msa0476160:CDS 55.0%
! GATGGCTTCGTCAAGAGTGC+TGG + chr3_3:79931580-79931599 None:intergenic 55.0%
CCTAGTAGGGTTTCGCTCGC+TGG + chr3_3:79931093-79931112 None:intergenic 60.0%
CCAGCGAGCGAAACCCTACT+AGG - chr3_3:79931090-79931109 Msa0476160:three_prime_UTR 60.0%
CGCCGCGGAAACGGAGAAAA+AGG - chr3_3:79931179-79931198 Msa0476160:three_prime_UTR 60.0%
GGATCGGGAGACTCGAGAGA+AGG - chr3_3:79931200-79931219 Msa0476160:three_prime_UTR 60.0%
TCGGGAGACTCGAGAGAAGG+AGG - chr3_3:79931203-79931222 Msa0476160:three_prime_UTR 60.0%
CGCCAAGAAGAGGGAGGAAG+AGG - chr3_3:79931266-79931285 Msa0476160:three_prime_UTR 60.0%
CGCCTCTTCCTCCCTCTTCT+TGG + chr3_3:79931271-79931290 None:intergenic 60.0%
AGAGGCGGATAAGAAGGCGG+AGG - chr3_3:79931284-79931303 Msa0476160:three_prime_UTR 60.0%
GGAGCTGGAGGAGAAAGAGC+TGG - chr3_3:79931341-79931360 Msa0476160:intron 60.0%
CCTCCGTCACCTTCGGTACA+GGG + chr3_3:79931413-79931432 None:intergenic 60.0%
!! TGTACCGAAGGTGACGGAGG+CGG - chr3_3:79931413-79931432 Msa0476160:intron 60.0%
GCGAGAGGAGGAGCAAGCTA+TGG - chr3_3:79931449-79931468 Msa0476160:CDS 60.0%
AGGCGGAGGAAGCGAAGAAG+AGG - chr3_3:79931481-79931500 Msa0476160:CDS 60.0%
GAAGAAGAGGACTGCTGGGG+AGG - chr3_3:79931494-79931513 Msa0476160:CDS 60.0%
! GGAGGAGGAGTACGAGAGAG+TGG - chr3_3:79931512-79931531 Msa0476160:CDS 60.0%
GCGCCTCAAGGCTTCCTTCA+AGG - chr3_3:79931650-79931669 Msa0476160:CDS 60.0%
CCTAGTAGGGTTTCGCTCGC+TGG + chr3_3:79931093-79931112 None:intergenic 60.0%
CCAGCGAGCGAAACCCTACT+AGG - chr3_3:79931090-79931109 Msa0476160:three_prime_UTR 60.0%
CGCCGCGGAAACGGAGAAAA+AGG - chr3_3:79931179-79931198 Msa0476160:three_prime_UTR 60.0%
GGATCGGGAGACTCGAGAGA+AGG - chr3_3:79931200-79931219 Msa0476160:three_prime_UTR 60.0%
TCGGGAGACTCGAGAGAAGG+AGG - chr3_3:79931203-79931222 Msa0476160:three_prime_UTR 60.0%
CGCCAAGAAGAGGGAGGAAG+AGG - chr3_3:79931266-79931285 Msa0476160:three_prime_UTR 60.0%
CGCCTCTTCCTCCCTCTTCT+TGG + chr3_3:79931271-79931290 None:intergenic 60.0%
AGAGGCGGATAAGAAGGCGG+AGG - chr3_3:79931284-79931303 Msa0476160:three_prime_UTR 60.0%
GGAGCTGGAGGAGAAAGAGC+TGG - chr3_3:79931341-79931360 Msa0476160:intron 60.0%
CCTCCGTCACCTTCGGTACA+GGG + chr3_3:79931413-79931432 None:intergenic 60.0%
!! TGTACCGAAGGTGACGGAGG+CGG - chr3_3:79931413-79931432 Msa0476160:intron 60.0%
GCGAGAGGAGGAGCAAGCTA+TGG - chr3_3:79931449-79931468 Msa0476160:CDS 60.0%
AGGCGGAGGAAGCGAAGAAG+AGG - chr3_3:79931481-79931500 Msa0476160:CDS 60.0%
GAAGAAGAGGACTGCTGGGG+AGG - chr3_3:79931494-79931513 Msa0476160:CDS 60.0%
! GGAGGAGGAGTACGAGAGAG+TGG - chr3_3:79931512-79931531 Msa0476160:CDS 60.0%
GCGCCTCAAGGCTTCCTTCA+AGG - chr3_3:79931650-79931669 Msa0476160:CDS 60.0%
!! ATTCTAAGGCCGAGGCGGCG+AGG - chr3_3:79931145-79931164 Msa0476160:three_prime_UTR 65.0%
!! TTCTAAGGCCGAGGCGGCGA+GGG - chr3_3:79931146-79931165 Msa0476160:three_prime_UTR 65.0%
!! GGAGGAGCAGTACTGGCGAG+AGG - chr3_3:79931221-79931240 Msa0476160:three_prime_UTR 65.0%
GGCGGATAAGAAGGCGGAGG+CGG - chr3_3:79931287-79931306 Msa0476160:three_prime_UTR 65.0%
GGATAAGAAGGCGGAGGCGG+CGG - chr3_3:79931290-79931309 Msa0476160:three_prime_UTR 65.0%
! ACGAGCGGAGGTGCGTCGAT+TGG - chr3_3:79931317-79931336 Msa0476160:three_prime_UTR 65.0%
!! AGCGGAGGTGCGTCGATTGG+CGG - chr3_3:79931320-79931339 Msa0476160:three_prime_UTR 65.0%
! CCCTGTACCGAAGGTGACGG+AGG - chr3_3:79931410-79931429 Msa0476160:intron 65.0%
GCCTCCGTCACCTTCGGTAC+AGG + chr3_3:79931414-79931433 None:intergenic 65.0%
AGCTCCGCCTCCGTCACCTT+CGG + chr3_3:79931420-79931439 None:intergenic 65.0%
GAAGAGGACTGCTGGGGAGG+AGG - chr3_3:79931497-79931516 Msa0476160:CDS 65.0%
CTCTCTGGATGCCTGTCGGG+AGG + chr3_3:79931630-79931649 None:intergenic 65.0%
CTCCCGACAGGCATCCAGAG+AGG - chr3_3:79931628-79931647 Msa0476160:intron 65.0%
CGCCTCTCTGGATGCCTGTC+GGG + chr3_3:79931633-79931652 None:intergenic 65.0%
GCGCCTCTCTGGATGCCTGT+CGG + chr3_3:79931634-79931653 None:intergenic 65.0%
!! GCATCCAGAGAGGCGCCTCA+AGG - chr3_3:79931638-79931657 Msa0476160:intron 65.0%
! GAAGCCTTGAGGCGCCTCTC+TGG + chr3_3:79931645-79931664 None:intergenic 65.0%
!! ATTCTAAGGCCGAGGCGGCG+AGG - chr3_3:79931145-79931164 Msa0476160:three_prime_UTR 65.0%
!! TTCTAAGGCCGAGGCGGCGA+GGG - chr3_3:79931146-79931165 Msa0476160:three_prime_UTR 65.0%
!! GGAGGAGCAGTACTGGCGAG+AGG - chr3_3:79931221-79931240 Msa0476160:three_prime_UTR 65.0%
GGCGGATAAGAAGGCGGAGG+CGG - chr3_3:79931287-79931306 Msa0476160:three_prime_UTR 65.0%
GGATAAGAAGGCGGAGGCGG+CGG - chr3_3:79931290-79931309 Msa0476160:three_prime_UTR 65.0%
! ACGAGCGGAGGTGCGTCGAT+TGG - chr3_3:79931317-79931336 Msa0476160:three_prime_UTR 65.0%
!! AGCGGAGGTGCGTCGATTGG+CGG - chr3_3:79931320-79931339 Msa0476160:three_prime_UTR 65.0%
! CCCTGTACCGAAGGTGACGG+AGG - chr3_3:79931410-79931429 Msa0476160:intron 65.0%
GCCTCCGTCACCTTCGGTAC+AGG + chr3_3:79931414-79931433 None:intergenic 65.0%
AGCTCCGCCTCCGTCACCTT+CGG + chr3_3:79931420-79931439 None:intergenic 65.0%
GAAGAGGACTGCTGGGGAGG+AGG - chr3_3:79931497-79931516 Msa0476160:CDS 65.0%
CTCTCTGGATGCCTGTCGGG+AGG + chr3_3:79931630-79931649 None:intergenic 65.0%
CTCCCGACAGGCATCCAGAG+AGG - chr3_3:79931628-79931647 Msa0476160:intron 65.0%
CGCCTCTCTGGATGCCTGTC+GGG + chr3_3:79931633-79931652 None:intergenic 65.0%
GCGCCTCTCTGGATGCCTGT+CGG + chr3_3:79931634-79931653 None:intergenic 65.0%
!! GCATCCAGAGAGGCGCCTCA+AGG - chr3_3:79931638-79931657 Msa0476160:intron 65.0%
! GAAGCCTTGAGGCGCCTCTC+TGG + chr3_3:79931645-79931664 None:intergenic 65.0%
CGTTTCCGCGGCGCTCTTGC+GGG + chr3_3:79931172-79931191 None:intergenic 70.0%
CCGTTTCCGCGGCGCTCTTG+CGG + chr3_3:79931173-79931192 None:intergenic 70.0%
!! CCGCAAGAGCGCCGCGGAAA+CGG - chr3_3:79931170-79931189 Msa0476160:three_prime_UTR 70.0%
AGCCACGCGCCGCCAAGAAG+AGG - chr3_3:79931256-79931275 Msa0476160:three_prime_UTR 70.0%
GCCACGCGCCGCCAAGAAGA+GGG - chr3_3:79931257-79931276 Msa0476160:three_prime_UTR 70.0%
TCCCTCTTCTTGGCGGCGCG+TGG + chr3_3:79931261-79931280 None:intergenic 70.0%
ACGCGCCGCCAAGAAGAGGG+AGG - chr3_3:79931260-79931279 Msa0476160:three_prime_UTR 70.0%
!! GGTGCGTCGATTGGCGGAGC+TGG - chr3_3:79931326-79931345 Msa0476160:exon 70.0%
!! GCGTCGATTGGCGGAGCTGG+AGG - chr3_3:79931329-79931348 Msa0476160:exon 70.0%
!! AGGTGACGGAGGCGGAGCTG+AGG - chr3_3:79931421-79931440 Msa0476160:intron 70.0%
CGGAGGCGGAGCTGAGGAAG+CGG - chr3_3:79931427-79931446 Msa0476160:intron 70.0%
GGAGCTGAGGAAGCGGCGAG+AGG - chr3_3:79931434-79931453 Msa0476160:intron 70.0%
GCTGAGGAAGCGGCGAGAGG+AGG - chr3_3:79931437-79931456 Msa0476160:intron 70.0%
CGTTTCCGCGGCGCTCTTGC+GGG + chr3_3:79931172-79931191 None:intergenic 70.0%
CCGTTTCCGCGGCGCTCTTG+CGG + chr3_3:79931173-79931192 None:intergenic 70.0%
!! CCGCAAGAGCGCCGCGGAAA+CGG - chr3_3:79931170-79931189 Msa0476160:three_prime_UTR 70.0%
AGCCACGCGCCGCCAAGAAG+AGG - chr3_3:79931256-79931275 Msa0476160:three_prime_UTR 70.0%
GCCACGCGCCGCCAAGAAGA+GGG - chr3_3:79931257-79931276 Msa0476160:three_prime_UTR 70.0%
TCCCTCTTCTTGGCGGCGCG+TGG + chr3_3:79931261-79931280 None:intergenic 70.0%
ACGCGCCGCCAAGAAGAGGG+AGG - chr3_3:79931260-79931279 Msa0476160:three_prime_UTR 70.0%
!! GGTGCGTCGATTGGCGGAGC+TGG - chr3_3:79931326-79931345 Msa0476160:exon 70.0%
!! GCGTCGATTGGCGGAGCTGG+AGG - chr3_3:79931329-79931348 Msa0476160:exon 70.0%
!! AGGTGACGGAGGCGGAGCTG+AGG - chr3_3:79931421-79931440 Msa0476160:intron 70.0%
CGGAGGCGGAGCTGAGGAAG+CGG - chr3_3:79931427-79931446 Msa0476160:intron 70.0%
GGAGCTGAGGAAGCGGCGAG+AGG - chr3_3:79931434-79931453 Msa0476160:intron 70.0%
GCTGAGGAAGCGGCGAGAGG+AGG - chr3_3:79931437-79931456 Msa0476160:intron 70.0%
! CTTGCGGGCCCTCGCCGCCT+CGG + chr3_3:79931157-79931176 None:intergenic 80.0%
!!! GAGGGCCCGCAAGAGCGCCG+CGG - chr3_3:79931164-79931183 Msa0476160:three_prime_UTR 80.0%
! GGAGGCGGCGGCACGACGAG+CGG - chr3_3:79931302-79931321 Msa0476160:three_prime_UTR 80.0%
! CTTGCGGGCCCTCGCCGCCT+CGG + chr3_3:79931157-79931176 None:intergenic 80.0%
!!! GAGGGCCCGCAAGAGCGCCG+CGG - chr3_3:79931164-79931183 Msa0476160:three_prime_UTR 80.0%
! GGAGGCGGCGGCACGACGAG+CGG - chr3_3:79931302-79931321 Msa0476160:three_prime_UTR 80.0%
! GGCGGCGGCACGACGAGCGG+AGG - chr3_3:79931305-79931324 Msa0476160:three_prime_UTR 85.0%
! GGCGGCGGCACGACGAGCGG+AGG - chr3_3:79931305-79931324 Msa0476160:three_prime_UTR 85.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 79931031 79932285 79931031 ID=Msa0476160;Name=Msa0476160
chr3_3 mRNA 79931031 79932285 79931031 ID=Msa0476160-mRNA-1;Parent=Msa0476160;Name=Msa0476160-mRNA-1;_AED=0.05;_eAED=0.05;_QI=92|1|1|1|1|1|3|311|227
chr3_3 exon 79931031 79931356 79931031 ID=Msa0476160-mRNA-1:exon:21752;Parent=Msa0476160-mRNA-1
chr3_3 exon 79931447 79931566 79931447 ID=Msa0476160-mRNA-1:exon:21751;Parent=Msa0476160-mRNA-1
chr3_3 exon 79931645 79932285 79931645 ID=Msa0476160-mRNA-1:exon:21750;Parent=Msa0476160-mRNA-1
chr3_3 five_prime_UTR 79932194 79932285 79932194 ID=Msa0476160-mRNA-1:five_prime_utr;Parent=Msa0476160-mRNA-1
chr3_3 CDS 79931645 79932193 79931645 ID=Msa0476160-mRNA-1:cds;Parent=Msa0476160-mRNA-1
chr3_3 CDS 79931447 79931566 79931447 ID=Msa0476160-mRNA-1:cds;Parent=Msa0476160-mRNA-1
chr3_3 CDS 79931342 79931356 79931342 ID=Msa0476160-mRNA-1:cds;Parent=Msa0476160-mRNA-1
chr3_3 three_prime_UTR 79931031 79931341 79931031 ID=Msa0476160-mRNA-1:three_prime_utr;Parent=Msa0476160-mRNA-1
Gene Sequence

>Msa0476160

ATGCCCAAAAAGATGGGTGTGAATTCTAAGGCCGAGGCGGCGAGGGCCCGCAAGAGCGCCGCGGAAACGGAGAAAAAGGATCGGGAGACTCGAGAGAAGGAGGAGCAGTACTGGCGAGAGGCAGAGAGTTCAAAGCCACGCGCCGCCAAGAAGAGGGAGGAAGAGGCGGATAAGAAGGCGGAGGCGGCGGCACGACGAGCGGAGGTGCGTCGATTGGCGGAGCTGGAGGAGAAAGAGCTGGAGAAGATGATTAAAAAGCCTGACAAGAAGGCAAACAGGGTGTCTATCCCTGTACCGAAGGTGACGGAGGCGGAGCTGAGGAAGCGGCGAGAGGAGGAGCAAGCTATGGCACAGAAGAAGGCGGAGGAAGCGAAGAAGAGGACTGCTGGGGAGGAGGAGTACGAGAGAGTGGTGCTTGTGTCGAATACGAATCGTGATGATTCAATTATCGAAGCCAGCACTCTTGACGAAGCCATCGCTCAGATGAGTATCGATAACAACTTACCTCCCGACAGGCATCCAGAGAGGCGCCTCAAGGCTTCCTTCAAGGCATTTGAAGAAGCTGAACTACCTAAGTTGAAGCAAGAGAAACCAGGGCTTACATACACACAGTACAAGGACATGATTTGGAAGCTTTGGAAGAAATCTCCTGACAATCCTCTTAATCAAATTGCTGCCGAGTGA
Protein sequence

>Msa0476160

MPKKMGVNSKAEAARARKSAAETEKKDRETREKEEQYWREAESSKPRAAKKREEEADKKAEAAARRAEVRRLAELEEKELEKMIKKPDKKANRVSIPVPKVTEAELRKRREEEQAMAQKKAEEAKKRTAGEEEYERVVLVSNTNRDDSIIEASTLDEAIAQMSIDNNLPPDRHPERRLKASFKAFEEAELPKLKQEKPGLTYTQYKDMIWKLWKKSPDNPLNQIAAE*